KR20130137336A - Bead composition for a filter of water softener or water generator for skin, and water softener or water generator for skin comprising thereof - Google Patents

Abstract

The present invention relates to a bead composition for filtering water softener or beauty water generator for skin comprising same, wherein herb medicine extract and marine algae extract are collected by hydrophilic polymer for said bead composition, and said bead composition is applied to a functional filter. The water softener or beauty water generator for skin equipped with the bead composition delivers soft water containing dissolved herb medicine and active composition of marine algae to skin, thereby ensuring effects of anti-inflammation, skin elasticity, wrinkle prevention, skin generation, skin trouble, skin moisturization that herb medicine and marine algae have and preventing dead skin cells.

Description

Bead composition for water filter or water softener with water softener or water softener

The present invention is a softener or skin beauty filter that has the effect of eluting the active ingredient for a long time to prevent skin keratin with anti-inflammatory effect, skin elasticity and wrinkle improvement effect, skin regeneration effect, skin trouble reduction, moisturizing and skin elasticity increase effect The present invention relates to a bead composition and a water softener or skin care machine having the same.

Hardness components contained in hard water, such as tap water, are a factor that adversely affects life, such as various skin problems, reduced washing efficiency, fiber wear, pipe corrosion, and scale. The water softener is used to remove hardness components such as calcium, magnesium and iron dissolved in water.

The water softener removes the hardness-induced components contained in the water through a filter composed of an ion exchange resin inside, and as a result, the soft water is supplied to the skin, thereby minimizing the destruction of the skin barrier and strengthening the skin barrier recovery, thereby minimizing moisture loss. Can be.

Recently, a method for providing high-quality soft water, such as a pre-treatment filter for removing various rust residues or suspended matters or a functional filter added with vitamins or various active ingredients, has been proposed before or after the ion exchange resin.

The pretreatment filter is used to remove residual chlorine contained in the tap water. The pretreatment filter is installed inside the water softener so that the tap water can be treated before the water passes through the ion exchange resin.

In the case of the functional filter, the applicant is equipped with a vitamin receptor on the upper part of the water softener so that the chlorine component contained in the raw water can be largely removed when the water-soluble vitamin is dissolved in the raw water through Korean Patent Registration No. 10-0844528. It has been suggested that chlorine oxidation and removal can be accelerated by the dissolved vitamins to improve the performance and extend the life of ion exchange resins.

In addition, the Republic of Korea Patent Publication No. 2011-0064847 is a 99.9% purity vitamin and peach branch, plum tree branch, mulberry branch, lump tree branch, willow branch prepared by mixing at least one It is mentioned that water softener filters are effective for skin diseases such as atopy and can reduce skin irritation.

In addition, the Republic of Korea Patent Publication No. 2011-0059517 proposes that the night extract or fractions thereof have anti-inflammatory activity and have a prophylactic and therapeutic effect on inflammatory diseases, in particular, atopy when used as a water softener.

Republic of Korea Patent Registration No. 10-0844528 Republic of Korea Patent Publication No. 2011-0064847 Republic of Korea Patent Publication No. 2011-0059517

Accordingly, the present inventors have screened various natural ingredients having active ingredients beneficial to the skin, and as a result, obtains extracts by fermenting herbal medicines and seaweeds, and the extracts have anti-inflammatory activity, wrinkle improvement effect, skin elasticity improvement and regeneration effect. Excellent, rat-based experiments have shown that it has the effect of relieving atopic dermatitis and alleviating skin problems.

In order to apply these extracts to a product such as softeners or skin care machines, it is collected as a hydrophilic polymer and manufactured in the form of beads and used as a filter of the softener. As a result, the active ingredient of the extract is gradually released over a long period of time. The present invention was completed by confirming that the water softener can be used every time.

Accordingly, the present invention is to provide a water softener filter bead composition and water softener or skin care device having the same to alleviate skin problems such as atopic dermatitis, have a skin regeneration effect, and can be used for a long time in a stabilized form. do.

In order to achieve the above object, the present invention provides a bead composition for water softener or skin beauty water filter, the herbal extract and seaweed extract is collected by a hydrophilic polymer.

In addition, the present invention provides a hollow cylindrical housing having an inlet connected to the raw water pipe and an outlet connected to the outlet pipe and a discharge port for discharging the concentrated water;

It is composed of an ion exchange resin filter is filled between the inlet and the outlet of the inside of the housing to soften the raw water introduced into the inlet,

A functional filter is disposed between the raw water pipe of the inlet and the ion exchange resin filter,

The water softener or skin care machine comprising the functional filter equipped with a bead composition for a water softener or skin care machine filter according to the present invention in the housing.

The mixed extract bead composition according to the present invention may be applied to a water softener or skin beauty water, and the treated water passing through the water softener or skin beauty water is effective against skin diseases such as atopic dermatitis, including the active ingredients of herbal medicine and seaweed By reducing the use of soft water can increase the skin disease treatment and skin cosmetic effects.

In particular, the bead composition is capable of 100% capture without loss of the active ingredient in the mixed extract, and when used in a water softener or skin care machine can be commercialized as a product that can be used for a long time as the active ingredient of the extract is gradually released.

1 is a flow chart showing a manufacturing process of the bead composition for the filter according to the present invention.
2 is the RRLC-MS / MS spectrum of fermented Soybean extract, unfermented Soybean extract, and fucosterol.
3 is a view showing the expression levels of Type-1 procollagen and MMP-1 of the mixed extract of ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and ginseng: 톳 extract in a weight ratio of 1: 9. .
Figure 4 is a graph showing the inhibition of the elastase activity of the mixed extract of ursolic acid, ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and ginseng: 톳 extract in a weight ratio of 1: 9.
Figure 5 is a graph showing the change in the growth rate of human keratinocytes of the ginseng extract of Preparation Example 1, the extract of Preparation Example 2, and the mixed extract of ginseng: 톳 extract in a weight ratio of 1: 9.
6 is a photograph showing a change in the trouble experiment using the atopic dermatitis induced animal model used in this experiment.
Figure 7 is a graph showing the number of scratches of the mouse when treated with a control, positive control (dexamethasone), the mixed extract according to the present invention.
Figure 8 (a) is a control, positive control (dexamethasone), a cross-sectional picture of the skin layer of the mouse when treated with the mixed extract according to the present invention, (b) is a graph showing the skin thickness.
Figure 9 (a) is a control, positive control (dexamethasone), a photograph showing the mast cells when treated with a mixed extract according to the present invention, (b) is a graph showing the results of measuring the number of mast cells.
10 is a graph showing the content of immunoglobulin E in serum when treated with a control, a positive control (dexamethasone), a mixed extract according to the present invention.
Figure 11 is a photograph showing the mixed extract beads prepared in Preparation Example 3.
12 is a photograph showing a mixed extract bead form according to the alginate content.
13 is a graph showing the change in absorbance of the eluate measured for 28 days.
Figure 14 is a TLC photograph showing the component analysis of soft water subjected to a continuous acceleration test, (a) ginseng extract, (b) shows the components related to the extract.
15 is a graph showing transdermal moisture loss using soft water produced by the control, F yarn mist, and beads according to the present invention.
Figure 16 is a graph showing the skin barrier recovery rate using soft water produced by the control, F yarn mist, and beads according to the present invention.

Hereinafter, the present invention will be described in more detail.

The filter composition according to the present invention includes herbal extracts and seaweed extracts, which are collected in a hydrophilic polymer and prepared in the form of beads (see FIG. 1).

Herbal medicine and algae used as raw materials select raw materials whose active ingredients have a beneficial effect on the skin, and use extracted extracts to maximize the active ingredients.

Herbal extracts can be used as long as it is known to have a beneficial effect on the skin, but ginseng, red ginseng, Angelica, Peony, peppermint, eoseongcho, three hundred seconds, sewage, Cheongung, licorice, earthenware, passer, Baekbongryeong, Baekgangjam, and their The one obtained from one herbal medicine selected from the group consisting of a combination is used, preferably ginseng.

For example, ginseng used as a herbal medicine raw material contains a large amount of active ingredients such as Rb1, Rc, Rd, and F2 in ginseng, and these ingredients have an anti-inflammatory effect, skin elasticity and wrinkle improvement, and also a skin regeneration effect. great.

In addition, the seaweed extract may be used that is used in the field of cosmetics or external preparations, one seaweed selected from the group consisting of 톳, Ecklonia cava, moss, green grass, hearing, seaweed, kelp, seaweed, gompi, hearing, and combinations thereof. It is possible to obtain from these, preferably using 톳.

Soybean contains rich nutritional ingredients such as organic minerals. Especially, Phlorotannin fractionation layer containing fucosterol in Soybean reduces skin trouble, increases moisturizing and skin elasticity, and exfoliates skin. It is effective to prevent.

These herbs and seaweeds are used in a dry extract state, but in order to achieve synergistic effect, the herbal extracts: seaweed extracts are mixed in a weight ratio of 1: 9-9: 1, preferably in a weight ratio of 7: 3-3: 7. . At this time, the content range of each extract may be changed depending on the type of herbal medicine and seaweed.

The medicinal herb and seaweed extract can be obtained by extraction using water or an organic solvent or a mixed solvent thereof. Preferably, the herbal medicine or algae pulverized by drying for a predetermined time is subjected to cold extraction, heat extraction, ultrasonic extraction, cooling extraction, and the like. According to the extraction method can be extracted by conventional extraction methods in the art.

At this time, the extraction method is not particularly limited, and may be extracted by room temperature or warming under conditions in which the active ingredient is not destroyed or minimized. However, the herbal medicine and seaweed extract according to the present invention can increase the amount of the active ingredient each component by using a fermentation extract obtained through the extraction after fermentation.

That is, in the case of ginseng to extract as much as possible the active ingredient of the minor saponin F2, in the case of 경우 also pulverized herbal medicine or algae before extraction so that the specific phlorotannin fractionation layer, for example, fucosterol as the active ingredient is extracted as much as possible It is obtained by fermenting the dried product obtained by using yeast mold, yeast or lactic acid bacteria to obtain a fermentation broth, and then extracting the fermentation components in the fermentation broth.

The fermentation process is distilled water in herbal or seaweed and basal medium (YM medium or MRS medium), Yeast mold, yeast or lactic acid bacteria are incubated for 24 to 120 hours while maintaining the conditions of inoculation (concentration of about 10 ^{5-10 7} cells / ml), pH 5-8, temperature 20-45 ℃. After centrifugation to remove the precipitate and yeast cells, yeast cells or lactic acid bacteria.

For fermentation, solvent is used as a base medium for distilled water, which is used to feed yeast mold, yeast or lactic acid bacteria.

The yeast fungus Aspergillus Niger ( Aspergillus) niger ) and Aspergillus arige ( Aspergillus) oryzae), Aspergillus terephthalate mouse (Aspergillus. terreus), Usami Aspergillus (Aspergillus. usamii), Aspergillus wenti (Aspergillus .wentii), Aspergillus fumigatus Fu (Aspergillus. fumigates) 1 of used paper ,

The yeast is Saccharomyces ( Saccharomyces) cerevisiae ), Saccharomyces boulardii ), Saccharomyces ellipsoids , and Schizosaccharomyces ellipsoids may be used.

In addition, Lactobacillus Lactobacillus ( Lactobacillus) acidophilus), Lactobacillus casei (Lactobacillus casei), Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus ), Lactobacillus rhamnosus ), Lactobacillus planta ( Lactobacillus plantarum ), Lactobacillus fermentum), Lactobacillus brevis (Lactobacillus brevis ), Lactobacillus 1 species is used from the group which consists of reuteri ) and Lactobacillus confusus .

These extracts are extracted after fermentation to increase the skin absorption due to the generation of new potent ingredients and low molecular weight of the active ingredient, which is more useful than the extracts.

Preferably, in order to simplify the fermentation process, after the liquid culture of the strain to prepare a coenzyme by protein precipitation method using an organic solvent such as ethanol, acetone, or ammonium sulphate (ammonium sulphate) and added to the pulverized herbal medicine or seaweed It can be processed.

Usable extraction methods can be prepared by methods such as hot water extraction, room temperature extraction, warm extraction, ultrasonic extraction, supercritical extraction commonly used in this field.

Herbal medicine and seaweed fermented extract of the present invention is prepared by known extraction methods known to those skilled in the art. For example, the herbal and seaweed fermented extracts of the present invention are used as fermentation broth obtained through fermentation, or after drying the fermentation broth.

The extraction solvent may include water, lower glycols having 1 to 6 carbon atoms, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, methylene chloride, chloroform, benzene, toluene, dimethylacetamide, N-methylpyrrolidone, and dimethyl sulfoxide. 1 type selected from the group consisting of a side, a xylene, benzene, n-butyl acetate, methylcyclohexane, dimethylcyclohexane, hexane, acetone, and a mixed solvent thereof is used. Preferably, organic solvents such as water, methanol, ethanol or acetone, ethyl acetate and chloroform and mixed solvents thereof are used to remove odors peculiar to herbal medicines and seaweeds.

The extraction solvent is used in a volume of 1 to 20 times the dry weight, and extracted by heating at 30 to 100 ° C. for 3 to 48 hours or by depositing at 5 to 30 ° C. for 1 to 5 days to extract the active ingredient. Obtained by concentration under reduced pressure concentrator.

In addition, it can be prepared using a known ultrasonic extraction method, etc., extracted for 1 to 12 hours with an ultrasonic extractor in the extraction solvent at room temperature. In this case, the time of the ultrasonic extraction may vary depending on the amount of the sample to be extracted, and may undergo a process of filtering or purifying the extraction result.

The extract obtained after extraction is prepared in powder form by hot air drying, spray drying or freeze drying.

In order to apply the herbal and seaweed extracts obtained through the fermentation extraction process as described above, the present invention collects these mixed extracts in the form of beads with hydrophilic polymers in order to be applied to water softeners and skin care filters.

The hydrophilic polymer collects 100% herbal medicine and seaweed extract, and allows the preparation of sustained-release beads so that the herbal medicine and seaweed extract components are melted at once when contacted with water to be slowly released by the hydrophilic polymer without being eluted.

Such hydrophilic polymers include cellulose derivatives of alginate, liposomes, gelatin, carboxy methyl cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose; Polysaccharides of hyaluronic acid, alginic acid, polysaccharides, heteropolysaccharides (pectin); Poloxamer, poloxamine, ethylene vinyl acetate, polyethylene glycol, dextran, polyvinyl pyrrolidone, chitosan, polyvinyl alcohol, polyvinylacetate, phosphatidylcholine (lectin), polylactic acid, polyhydroxybutyric acid, methacrylic acid One kind selected from the group consisting of a copolymer, polyethylene glycol-polyhydroxybutyric acid (PEG-PHB), polyvinylpyrrolidone-polyvinyl alcohol (PVP-PVA), and a combination thereof is preferably used. Alginate or liposomes are used.

In the embodiment of the present invention, alginate was used to enclose the herbal medicine and the seaweed extract. In other words, when the herbal medicine or seaweed extract is directly powdered or granulated, it is impossible to prepare it in such a way that it dissolves at the time of contact with water and is released slowly over a long period of time. Thus, in the case of collecting the alginate and preparing it in the form of beads as in the present invention, it is possible to obtain the effect of slowly releasing the contents over a long time without melting the contents at the time of contact with water.

Alginate is a major component of the cell walls of brown algae and algae, a type of polysaccharide. It has excellent biocompatibility and is a biodegradable molecule and has various physical properties, so it is widely used as a additive for medicine, cosmetics, and food. Not only is there no toxicity and no secondary pollution, it is less likely to be deteriorated by the reaction of microorganisms during storage and storage, and can be completely dissolved in water after a certain time.

Beads prepared using the hydrophilic polymer including the alginate have a spherical shape and do not adversely affect the water quality, and the bioactive substance can be kept in a stable state and slowly release the bioactive substance over a long period of time. It is effective to let.

At this time, the collection is not particularly limited in the present invention, a method known in the art may be used. Specifically, a hydrophilic polymer is swelled in a solvent to prepare a solution, and when the solution is dropped into the mixed extract solution of the herbal medicine extract and seaweed extract, beads are formed, and the formed beads are washed and dried to prepare mixed extract beads.

As the solvent required for the swelling of the hydrophilic polymer, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used, and a salt may be added if necessary. For example, when alginate is used as a hydrophilic polymer, calcium chloride is added together with water to prepare 0.3 M ion exchange solution.

The solvent used for dissolving the herbal medicine and seaweed extract may also be water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and if necessary, they are used by performing pretreatment. For example, in the case of seaweed extract, a unique fishy smell occurs. In the previous extraction process, pretreatment is performed by using an organic solvent such as ethanol, methanol or acetone, ethyl acetate, and chloroform and a mixed solvent thereof.

The content of the hydrophilic polymer and herbal medicine and seaweed extract used in the collection may vary depending on the capturing ability for the mixed extract of the hydrophilic polymer, preferably in the range of 10 to 20 parts by weight of the mixed extract based on 100 parts by weight of the hydrophilic polymer Used by If the content of the mixed extract is less than the above range, the effect of the active ingredient may not be sufficiently secured. If the content of the mixed extract exceeds the above range, bead production may be impossible beyond the capture limit of the hydrophilic polymer.

In particular, beads are prepared through a drop of a hydrophilic polymer solution in a mixed extract solution, wherein bead formation can be controlled according to the concentration of the hydrophilic polymer solution. In order to obtain spherical beads, the hydrophilic polymer solution is preferably prepared at a concentration of 1 to 2% (g / v). If the concentration of the hydrophilic polymer is less than the above range, the viscosity of the polymer solution is less viscous due to the swelling degree of the polymer solution, so that even if the beads are not formed or formed, it is difficult to be spherical. Therefore, it uses suitably within the said range.

At this time, if necessary, various additives that can be beneficial to the skin and control the properties of the beads can be used. The additive may be a physiologically active ingredient such as vitamins or moisturizers, emollients, preservatives, pH regulators, fatty acids, antioxidants, pigments, dyes, flavoring agents, stabilizers, etc., these additives are from ppm to 5% by weight in the total composition Use within the range.

Beads produced through the collection of hydrophilic polymers are dried after washing with distilled water, where drying may be performed by hot air or cold air drying. The drying conditions are not limited in the present invention, but since the shape of the beads may be changed after drying, there is almost no change in the shape of the beads, and the drying is carried out to a level that can be returned to its original shape when immersed in water. Preferably, drying is carried out for 30 to 1 hour so that the water content in the beads is about 60 to 90%.

The soft water filter beads according to the present invention manufactured through such a step have a uniform spherical shape with a diameter of 3 to 5 mm, as shown in FIG. To restore its appearance. In Figure 11, although the beads showed the apricot color, the color varies depending on the content ratio of the herbal extracts and seaweed extracts used, and the size of the beads can also be prepared by controlling the viscosity of the hydrophilic polymer solution.

The soft water filter beads have a sustained release characteristic of gradually releasing active ingredients in the herbal medicine and seaweed extract when contacted with water, and may be applied in various forms in a product.

The water softener includes a hollow cylindrical housing having an inlet connected to the raw water pipe, an outlet connected to the outlet pipe, and a discharge port through which the concentrated water is discharged;

It is composed of an ion exchange resin filter is filled between the inlet and the outlet of the inside of the housing to soften the raw water introduced into the inlet,

In the housing is placed a functional filter equipped with a bead composition for the filter according to the invention. The filter may be disposed both before and after the ion exchange resin. Preferably, the active ingredient related to skin care of the functional filter is gradually released over a long period of time, thereby reducing the effect of the extract. It is better to place it behind the ion exchange resin as it can be checked every time it is used.

If the functional filter is provided with a space for accommodating the inside of the filter is not significantly limited in its shape, and the flat cross-section is configured to have a circular shape so that the resistance is minimized when water flows into the water flows inside desirable.

The functional filter is applied in the form inserted in the ion exchange resin filter or manufactured separately and used in series with the ion exchange resin filter.

The functional water equipped with this functional filter is delivered to the skin by the contact of water and beads every time the water is dissolved in the active ingredients of the herbal medicine and algae in the bead, the anti-inflammatory effect of the skin of the herbal medicine and seaweed, skin elasticity and wrinkles In addition to improving skin, regenerating skin, reducing skin trouble, moisturizing and increasing skin elasticity, it is possible to prevent skin dead skin cells.

According to Experimental Example 6 of the present invention, even if 3 tons of water was used, the active ingredient contained in the beads was continuously released, and compared with the mist commercially available in Experimental Example 7, the amount of transdermal water loss was small and the skin barrier was almost the same level. It can be seen that the recovery. Therefore, the bead-filled functional filter can be commercialized by replacing only the beads after a certain time.

[Example]

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the present invention is not limited to these Examples and Experimental Examples.

Manufacturing example  1: Medicinal Herb Extract Preparation

Ginseng extract

10 kg of water was added to 1 kg of ginseng powder, and a commercially available Aspergillus niger- derived enzyme was added, followed by reaction at 55 ° C. for 24 hours. Subsequently, the reaction solution was heated to 90 ° C. to stop enzymatic activity, and 10 L of alcohol was added thereto, followed by extraction at 70 ° C. for 4 hours.

Subsequently, the extract was filtered, the filtrate was concentrated under reduced pressure to around 30 brix, and then lyophilized to obtain a powdered herbal extract.

Component analysis

The active ingredient of the prepared ginseng extract was measured by HPLC and shown in Table 1 below.

For HPLC analysis, a PDA HPLC instrument and an Eclipse XDB-C18 column (4.6 × 250 mm, ID 5 μm) were used, and the mobile phase was a mixed solvent of water / acetonitrile 75:25, 68:32, 45:55, 40: 60, 0: 100, 0: 100 (volume ratio), and extracts were collected at 5 minute intervals. At this time, the flow rate of the mobile phase was flowed at 1.2 ml / min, and the components of ginseng were analyzed at 203 nm using a UV detector.

number Retention time (minutes) ingredient %area Height One 31.875 Rb1 29.17 213310 2 32.735 Rc 13.86 99501 3 35.977 Rd 22.12 149010 4 39.618 F2 6.94 67605

Referring to Table 1, it can be seen that the ginseng extract contains the active ingredients of Rb1, Rc, Rd and F2.

Manufacturing example  2: Seaweed Extract Preparation

Top extract

1 10L of water was added to 1 kg of powder, and a commercially available Aspergillus niger- derived enzyme was added and mixed, followed by reaction at 60 ° C. for 24 hours. Subsequently, the reaction solution was heated to 100 ° C. for 1 hour to stop the enzyme activity, and 10 L of 95% alcohol was added thereto, followed by extraction at 30 ° C. for 24 hours.

Subsequently, the extract was filtered, the filtrate was concentrated under reduced pressure to around 30 brix and freeze-dried to obtain a powdery extract.

Component analysis

The obtained extract was analyzed by RRLC-MS / MS (Rapid resolution liquid chromatography-tandem mass spectrometry) using Agilent 6410B (Agilent Technologies, USA, Santa Clara CA) of Agilent Technologies. Indicated. At this time, the fermented non-fermented Soybean extract, which was not subjected to the fermentation process by enzymes, was analyzed together for comparison.

2 is the RRLC-MS / MS spectrum of fermented Soybean extract, unfermented Soybean extract, and fucosterol. Referring to Figure 2, it can be seen that by performing the fermentation process when obtaining the extract can effectively increase the content of fucosterol, the active ingredient of 톳.

Various In vitro and In In order to confirm the effect on the skin of the mixed extract of ginseng and 톳 presented in the present invention In vivo evaluation was performed and the results are shown below.

In vitro  Activity evaluation

Experimental Example  1: Mixed skin extracts, including skin inflammation trouble  Experiment

In this experiment, murine macrophage RAW 264.7 cells were distributed from Korean Cell Line Bank, Korea, and the experiment was performed. DMEM was used as a basal medium for cell growth of RAW 264.7. RAW 264.7 cells were cultured 2 × 10 ⁵ cells / ㎖ concentration of moist CO ₂ incubator were inoculated in T- flasks in _{(humidified CO 2 incubator) (5} % CO 2, 95% air) under the conditions of 37 ℃ 24 sigan . Then, after diluting the LPS at a concentration of 10 μg / ml in the medium without FBS and treating the cells for 24 hours, the cells were diluted with the drug at a concentration of 1 mg / ml in the medium without FBS. After 24 hours the cells were taken and used for the experiment.

The activity of the inducible nitric oxide synthase (iNOS) was measured by measuring the amount of nitric oxide (NO) produced. The amount of NO produced from Raw 264.7 cells was measured in the form of NO ₂ ^- in the cytoplasm using the Nitrate / Nitrite Colorimetric assay kit. It was quantified according to the instructions of the Cayman Chemical Company, the results obtained are shown in Table 2 below. At this time, as the experimental material was used a mixed extract of ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and ginseng: 톳 extract in a weight ratio of 1: 9. In addition, there was no cytotoxicity to RAW 264.7 cells at all experimental concentrations, and for comparison, a commercially available dexamethasone, a inflammatory drug, was used at a concentration of 1 ppm.

Sample Concentration (ppm) Generation (μM) LPS untreated group - 8.43 ± 0.40 LPS Inflammatory Group 0.1 43.53 ± 1.0 Dexamethasone 1 ppm 23.94 ± 1.1 Ginseng Extract of Preparation Example 1 0.1 ppm 23.94 ± 1.1 1.0 ppm 21.47 ± 0.52 10ppm 17.82 ± 1.51 50 ppm 15.33 ± 2.0 톳 extract of Preparation Example 2 0.1 ppm 16.59 ± 1.61 1.0 ppm 18.87 ± 1.85 10ppm 17.70 ± 0.30 50 ppm 16.22 ± 2.15 Ginseng / 톳 Mixed Extract 0.1 ppm 16.74 ± 0.12 1.0 ppm 13.40 ± 0.04 10ppm 12.33 ± 0.09 50 ppm 9.69 ± 0.54

Referring to Table 2, it was found that about 70% of NO extract was significantly inhibited in the case of the extract using a combination of ginseng and 톳 according to the present invention, compared to the case of treating LPS alone to cause inflammation. p <0.05).

Experimental Example  2: Experiment with Moisturizing and Skin Elasticity of Mixed Extracts

In order to examine the skin moisturizing and elasticity improvement effect, using the mixed extract of the ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and ginseng: 톳 extract in a weight ratio of 1: 9, (1) to The experiment of (3) was performed.

(1) fibroblasts ( fibroblast ) Test of proliferation effect

In order to determine the effect of the mixed extract on the skin wrinkles and elasticity, the cell proliferation rate (HDF-N proliferation rate) was confirmed after 48 hours using human-derived fibroblast cells, and the obtained results are shown in Table 3 below. It was.

Sample Concentration (/ / ml) Cell proliferation rate (%,
UV B not irradiated) Cell proliferation rate (%,
UV B irradiation) LPS untreated group - 100 69 Ginseng Extract of Preparation Example 1 0.01 ppm 100 75 0.1 ppm 94 86 1.0 ppm 103 97 10ppm 113 96 톳 extract of Preparation Example 2 0.01 ppm 98 68 0.1 ppm 101 78 1.0 ppm 107 94 10ppm 115 96 Ginseng / 톳 Mixed Extract 0.01 ppm 104 70 0.1 ppm 116 84 1.0 ppm 117 97 10ppm 127 106

Referring to Table 3, the ginseng / 삼 mixed extract according to the present invention can be seen that the cell proliferation rate of fibroblasts after 48 hours is high.

In addition, the control group was not treated after the UV B irradiation showed a tendency to decrease the cell growth rate compared to the group not irradiated with UV B, but the mixed extract according to the present invention showed a high cell growth rate at 10ppm . From these results, it can be seen that the active ingredient of the mixed extract according to the present invention has a protective effect of fibroblasts from UV B, and as a result, it can have a positive effect on skin wrinkle improvement and skin elasticity increase. .

(2) collagen biosynthesis effect and Collagenase  Activity inhibition assessment test

In order to examine the effects of the mixed extract on skin aging, UV B was irradiated to human fibroblast cells to confirm the effect on the expression levels of MMP-1 and Type-1 procollagen. 3 is shown.

3 is a view showing the expression levels of Type-1 procollagen and MMP-1 of the mixed extract of ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and ginseng: 톳 extract in a weight ratio of 1: 9. . In this case, as MMP-1 is increased and Type-1 procollagen expression is decreased, the degree of photoaging is increased.

Referring to FIG. 3, the expression of MMP-1 decreases when the mixture is used alone, and the expression of Type-1 procollagen is increased, and the mixed extract prevents photoaging of the skin and prevents damage to the skin. It was confirmed that there is an effect.

(3) Elastase  Activity inhibition assessment test

To determine the effect of the mixed extract on skin tissue elasticity, elastase was measured by measuring the amount of 4-nitroanilin at light wavelength 410 nm by a test based on the hydrolysis of the synthetic peptide substrate by elastase. Inhibition of the first activity was confirmed.

All were given a reaction time of 20 minutes, and tested using concentrations of 1.0, 10, and 100 ppm, with ursolic acid (100 ppm) as a positive control. After stopping the reaction with ice after the reaction is shown in Figure 4 the results obtained using a microplate reader.

Figure 4 is a graph showing the inhibition of the elastase activity of the mixed extract of ursolic acid, ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and a mixed extract in a weight ratio of 1: 9. Referring to FIG. 4, the mixed extract according to the present invention showed better activity than the case where each extract was used, and showed the effect of inhibiting elastase activity higher than ursolic acid at the same concentration (100 ppm). By increasing the elasticity of the skin was confirmed that can reduce the wrinkles.

Experimental Example 3 Experiment Including Regeneration of Mixed Extract and Prevention of Keratin

In order to determine the skin regeneration and the prevention of keratin preventive effect of the mixed extract according to the present invention, the cell proliferation rate of each extract was confirmed by MTT assay using human-derived keratinocytes (Keratinocyte).

Figure 5 is a graph showing the change in human keratinocyte proliferation rate of ginseng extract of Preparation Example 1, 톳 extract of Preparation Example 2, and mixed extract. Referring to Figure 5, the mixed extract according to the present invention can be seen that the activity is superior to the case of using each extract has a skin regeneration and preventing keratin.

In vivo  Activity evaluation

Experimental Example  4: Evaluation of Efficacy of Mixed Extract in Atopic Dermatitis-Induced Animal Models

In order to directly confirm the effect on the skin of the mixed extract according to the present invention, the experiment was performed using the Nc / Nga mouse model.

(1) dermatitis induction and sample processing

The experimental animals used in this experiment were 5 week-old NC / Nga mice, supplied from the central experimental animal, and adapted at the experimental animal breeding ground for 1 week, weighed (22 ± 3g), and 6-6 animals per group. Used for. Solid feed and water were ingested freely during the experimental period, and the temperature (21 ± 1 ℃) and humidity (50 ± 5%) of the feeding room were kept constant. One day before the start of the experiment, the entire back area from the bottom of the ear to the top of the tail was removed with a mouse hair removal clipper (JC-4005, JOAS Elec. CO., Korea). This animal experiment was conducted in accordance with the regulations of the Animal Experiment Ethics Committee with prior review by Kyung Hee University's Experimental Animal Ethics Committee.

(2) Induction of atopic dermatitis and sample treatment

24 hours later, 200 μl of 0.5% DNCB (Sigma 23732-9) solution (acetone: olive oil = 3: 1) was added for 2 weeks after immunization of the dorsal area of the mouse for atopic induction of Nc / Nga mice. It was applied to the back area, and from 3 weeks after the start of sample administration, 200 μl of 0.5% DNCB solution was applied to the back area three times a week to induce atopic dermatitis. After depilation of the mice and DNCB treatment for 2 weeks to induce atopy, water, dexamethasone (1 μg / kg), and mixed extracts (2 mg / kg), respectively, were started and treated daily for 4 weeks.

(3) sensory evaluation-visual lesion evaluation

The lesion site of the mouse in which the atopic dermatitis was induced was visually observed.

Figure 6 is a photograph showing the atopic dermatitis induced animal model used in this experiment. In this case, the normal group was a group that did not induce atopic dermatitis, and the control group means atopic dermatitis-induced group (distilled water treatment). For the comparison, the positive control group was treated with dexamethasone.

Referring to FIG. 6, at week 0, bleeding and abscess papules and bleeding in the entire dorsal area, severe dermatitis including bleeding and severe dermatitis, including swelling and bleeding in the auricle, are caused in humans. The skin lesions were very similar to those of atopic dermatitis.

At 2 weeks, the lesions were improved with moderate dermatitis with erythema and slight edema. At 4 weeks, the skin lesions of the mice using the mixed extract according to the present invention were improved to some extent compared to the negative control. You can see that it happened.

(4) scratching behavior ( Scratching Behavior ) evaluation

The number of scratches was measured immediately after inducing atopic dermatitis (0 weeks) for the control group, the dexamethasone treated group, and the mixed extract treated group. At this time, the scratching behavior of the hind legs was recorded as a scratching behavior, and a quick scratching behavior was also recorded once in a second. The obtained results are shown in FIG. 7.

Figure 7 is a control, positive control (dexamethasone), a graph showing the number of scratches of the mouse in the mixed extract treatment group according to the present invention. Referring to FIG. 7, the number of scratches of the mouse was greatly increased at 0 weeks immediately after inducing atopy, and decreased with time. After the application of dexamethasone and mixed extract at 2 weeks, the decrease was significantly decreased, and the mixed extract showed a more significant decrease tendency than dexamethasone.

(5) histological observation and skin thickness

In order to measure the thickness of the epidermal layer of the atopic induction / uninduced group, the skin tissue was observed by staining with Hematoxylin and Eosin (H & E).

Figure 8 (a) is a control, a positive control (dexamethasone), a cross-sectional picture of the skin layer of the mouse when treated with the mixed extract according to the present invention, (b) is a graph showing the skin thickness measured at this time.

Referring to Figure 8 (a), it can be seen that the thickness of the epidermal layer of the group treated with dexamethasone and the mixed extract is significantly reduced compared to the control.

Compared with the normal group having a thickness of 10.70 ± 1.09 μm, as shown in (b) of FIG. 8, the thickness of the epidermal layer was 29.94 ± 10.43 μm due to atopic induction in the control group, which was about three times or more thick, and a positive control group. In the case of the 24.81 ± 12.74 ㎛, the mixed extract treatment group was 16.81 ± 4.24 ㎛ thick thickness was greatly reduced, it was confirmed that the entire skin layer is stable and less cell infiltration.

(6) mast cells ( Mast cell Counting)

In skin tissues with symptoms of atopic dermatitis, mast cells are known to be present in a degranulated form from the dermal layer to the upper subcutaneous fat layer. In this experiment, the number of mast cells between the groups was compared by toluidan blue staining.

Figure 9 (a) is a control, positive control (dexamethasone), a photograph showing the mast cells treated with the mixed extract according to the present invention, (b) is a graph showing the results of measuring the number of mast cells.

Referring to Figure 9 (a), the control group caused by the induction of atopic dermatitis, the number of mast cells (arrow display area), the number is reduced when treated with dexamethasone and mixed extracts.

Referring to (b) of FIG. 9, which is specifically quantified, the number of mast cells in the normal group was measured to be 7.58 ± 5.72, and in the control group, 42.19 ± 18.58 because of the induction of atopy, about 5 times or more. In case of the dexamethasone treatment group, it was 29.41 ± 12.55, and in the mixed extract treatment group, it was 26.07 ± 9.06, which was reduced to 1/2 of the control group.

(7) immunity Globulin  E measurement

In order to measure the amount of immunoglobulin in serum used as an index for assessing allergic diseases such as atopic dermatitis, the amount of immunoglobulin E in serum was measured 4 weeks after atopic induction.

10 is a graph showing the content of immunoglobulin E in serum when treated with a control, a positive control (dexamethasone), a mixed extract according to the present invention. Referring to Figure 10, it can be seen that the content of immunoglobulin E is greatly reduced by the use of the mixed extract according to the present invention.

(8) Serum cytokine measurement

Atopic dermatitis is also linked to the inflammatory response, and inflammatory cytokine plays an important role in improving atopic disease. Therefore, the amount of IgE and IL-4 in mouse blood can be quantified using an ELISA kit. It is shown in Table 4 below.

TNF-a concentration IL-4 concentration Normal group 1.29 pg / ml 4.195 pg / ml Control group 5.365 pg / ml 5.545 pg / ml Positive control group 4.78 pg / ml 4.17 pg / ml Mixed extract 3.23 pg / ml 4.15 pg / ml

Referring to Table 4 above, the concentration of TNF-α was increased due to inflammation in the control group, but the concentration was significantly decreased when treated with dexamethasone and the mixed extract according to the present invention, which shows the same trend in the concentration of IL-4. , It can be seen that the use of the mixed extract according to the present invention enables the improvement of the atopic inflammatory response.

Experiments of Experimental Examples 1 to 4 confirmed the effects of the herbal medicine and seaweed extract according to the present invention, it was prepared bead form to apply to the water softener and skin beauty filter.

Manufacturing example  3: mixed extract Bead  Produce

The beads obtained by collecting the ginseng and 톳 extract prepared in Preparation Examples 1 and 2 as a hydrophilic polymer were prepared. At this time, alginate was used as the hydrophilic polymer.

0.45 g of ginseng and 4.05 g of ginseng were dissolved in 100 ml of water in a 500 ml beaker to prepare a mixed extract solution. Subsequently, 100 ml of calcium chloride aqueous solution having a concentration of 0.3 M was prepared, and an alginate solution containing 2% alginate was mixed thereto to prepare a carrier solution.

Spherical beads were molded while slowly dropping the carrier solution into the extract solution. After all the beads were molded and filtered, washed with distilled water, and then put in a mesh to remove excess moisture was used.

The final composition was to capture 4.5 g of seaweed extract and ginseng extract (4.5: 1 weight ratio) and 4.5 g of seaweed extract + ginseng extract into 30 g (weight) of alginate beads.

Figure 11 is a photograph showing the mixed extract beads prepared in Preparation Example 3. The manufactured beads have a spherical shape of 3 to 5 mm in diameter, and are elastic, so they contract and expand again when pressure is applied to restore the original shape.

Experimental Example  5: concentration of hydrophilic polymer solution Bead  Shape analysis

Beads were prepared by varying the amount of alginate used as the trapping material. Prepared in the same manner as in Preparation Example 3, wherein the concentration of the alginate solution was prepared by changing the concentration to 1%, 1.5% and 2%.

12 is a photograph showing a mixed extract bead form according to the alginate content. Referring to Figure 12, it can be seen that when the content of the alginate is too small or too large, the shape of the beads does not form a complete sphere, but is formed non-uniformly.

Experimental Example 6 : Mixed Extract Bead  Active ingredient dissolution analysis

(1) Check the dissolution

The mixed extract presented in the present invention was prepared in the form of beads and applied to a softener and skin care machine to check the dissolution degree of the active ingredient of the mixed extract.

After 60 g of beads were mixed with 200 ml of distilled water and mixed lightly, and after confirming the elution of the active ingredient using an absorbance meter, new distilled water was added and then measured for 28 days.

13 is a graph showing the change in absorbance of the eluate measured for 28 days. Referring to FIG. 13, the elution amount of the active ingredient was initially increased and gradually decreased, but after 4 weeks, the active ingredient was still eluted.

(2) Component Analysis Using Continuous Acceleration Test

It was confirmed that the active ingredient was eluted from the beads by the above (1), and the component analysis of the eluate was performed through this TLC analysis.

The mixed extract beads prepared in Preparation Example 3 were filled in a filter housing, and subjected to a continuous acceleration test under the condition of 10 LPM (10 L per minute), and 100 L of soft water before and after the time of 0.5t, 1.5t, 3t. Filtration was collected and concentrated.

Figure 14 is a TLC photograph showing the component analysis of soft water subjected to a continuous acceleration test, (a) ginseng extract, (b) shows the components related to the extract.

Referring to FIG. 14, there were F 2 components derived from ginseng extract and soft fucosterol component from the extract of soft ginseng, which still exist even when using a quantity of 3.0 tons.

Experimental Example  7: mixed extract Bead  Skin Barrier Recovery Characterization

Nude mice were used as skin moisturizing mouse models to measure changes in skin barrier recovery rates after treatment of distilled water (control), F company mist, and soft water (mixed extract beads of the present invention) after acute skin barrier injury. At this time, the skin of the mouse was acutely damaged using the tape stripping method, and then measured for 24 hours.

15 is a graph showing the transdermal water loss using the control group, F yarn mist, and soft water produced by the beads according to the present invention, Figure 16 is a graph showing the skin barrier recovery rate.

15 and 16, in the case of soft water produced by the beads according to the present invention can be seen to reduce the transdermal moisture loss more than 15%, increase the skin barrier recovery rate 10%, separate cosmetics such as mist It can be seen that the equivalent effect can be obtained without using.

Claims (22)

Bead composition for a water softener or skin beauty water filter, the herbal extract and seaweed extract is collected by a hydrophilic polymer. According to claim 1, The herbal extract is ginseng, red ginseng, Angelica, peony, peppermint, Eoseongcho, three hundred seconds, sewage, Cheongung, licorice, earthenware, pedestrians, Baekbongryeong, Baekgangjam, and combinations thereof Bead composition for a water softener or skin beauty hand filter, which is obtained from a herbal medicine. The water softener of claim 1, wherein the seaweed extract is obtained from one species of algae selected from the group consisting of 톳, Ecklonia cava, hatban, green, hearing, seaweed, kelp, seaweed, gompi, hearing, and combinations thereof. Or bead composition for skin beauty hand filter. The method of claim 1, wherein the hydrophilic polymer is alginate, liposomes, gelatin, carboxymethyl cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, cellulose derivative of hydroxypropyl methyl cellulose; Polysaccharides of hyaluronic acid, alginic acid, polysaccharides, heteropolysaccharides (pectin); Poloxamer, poloxamine, ethylene vinyl acetate, polyethylene glycol, dextran, polyvinyl pyrrolidone, chitosan, polyvinyl alcohol, polyvinylacetate, phosphatidylcholine (lectin), polylactic acid, polyhydroxybutyric acid, methacrylic acid A copolymer selected from the group consisting of polyethylene glycol-polyhydroxybutyric acid (PEG-PHB), polyvinylpyrrolidone-polyvinyl alcohol (PVP-PVA), and combinations thereof Bead composition for water softener or skin care filter. The bead composition of claim 1, wherein the bead composition comprises a mixed extract of the herbal medicine and seaweed in the range of 10 to 20 parts by weight based on 100 parts by weight of the hydrophilic polymer. The bead composition of claim 1, wherein the bead composition comprises a herbal extract: seaweed extract in a weight ratio of 1: 9 to 9: 1. According to claim 1, wherein the herbal medicine and seaweed extract
Ferment the herbal medicine or algae raw material with yeast mold, yeast or lactic acid bacteria to obtain fermentation broth.
Bead composition for a water softener or skin beauty hand filter, which is obtained by extracting the fermentation component in the fermentation broth. According to claim 7, wherein the yeast fungus Aspergillus niger ( Aspergillus niger ), Aspergillus duckling ( Aspergillus duck) with oryzae), Aspergillus terephthalate mouse (Aspergillus. terreus), Usami Aspergillus (Aspergillus. usamii), Aspergillus wenti (Aspergillus .wentii), Aspergillus fumigatus Fu (Aspergillus. fumigates), and combinations thereof A bead composition for a water softener or a skin beauty hand filter comprising one selected from the group consisting of. The method of claim 7, wherein the yeast is a Saccharomyces MRS three Levy cyano (Saccharomyces cerevisiae), saccharose in MRS bow radio (Saccharomyces boulardii ), Saccharomyces ellipsoids ), Schzosaccharomyces Ellipsoids ) and a bead composition for a water softener or skin beauty hand filter comprising at least one selected from the group consisting of these. According to claim 7, wherein the lactic acid bacteria Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus casei ( Lactobacillus casei ), Lactobacillus bulgaricus), Lactobacillus ramno Seuss (Lactobacillus rhamnosus), Lactobacillus Planta Room (Lactobacillus plantarum ), Lactobacillus fermentum), Lactobacillus brevis (Lactobacillus brevis ), Lactobacillus reuteri , Lactobacillus &lt; RTI ID = 0.0 &gt; confusus ) and a bead composition for a water softener or skin beauty hand filter comprising one selected from the group consisting of these. The method of claim 7, wherein the extraction solvent is water, lower C 1-6 glycol, lower C 1-4 alcohol, ethyl acetate, glycerin, methylene chloride, chloroform, benzene, toluene, dimethylacetamide, N-methylpi Water softener characterized by using one kind selected from the group consisting of rolidone, dimethyl sulfoxide, xylene, benzene, n-butyl acetate, methylcyclohexane, dimethylcyclohexane, hexane, acetone, and a mixed solvent thereof. Or bead composition for skin beauty hand filter. The bead composition of claim 7, wherein the extraction is performed through an extraction process of hot water extraction, room temperature extraction, warm extraction, ultrasonic extraction, or supercritical extraction. The method of claim 1, wherein the bead composition is prepared by swelling the hydrophilic polymer in a solvent to prepare a polymer solution, and extracting the extract solution obtained by dissolving the medicinal herb extract and seaweed extract in a solvent, respectively, by slowly dropping into the hydrophilic polymer solution. Bead composition for water softener or skin beauty hand filter. The bead composition of claim 13, wherein the hydrophilic polymer solution has a concentration of 1 to 2% (g / v). The bead composition of claim 1, wherein the bead composition has a water content of 60 to 90% in the bead. The bead composition of claim 1, wherein the bead composition has a diameter of 3 to 5 mm. The bead composition according to claim 1, wherein the bead composition collects the F2 component extracted from ginseng and the fucosterol extracted from ginseng with alginate. A hollow cylindrical housing having an inlet connected to the raw water pipe, an outlet connected to the outlet pipe, and a discharge port to discharge the concentrated water;
It is composed of an ion exchange resin filter is filled between the inlet and the outlet of the inside of the housing to soften the raw water introduced into the inlet,
A water softener or skin care machine comprising a functional filter in which the bead composition of claim 1 is mounted in the housing. 19. The water softener or skin care machine of claim 18, wherein the functional filter is disposed between the raw water pipe of the water inlet and the ion exchange resin filter. 19. The water softener or skin care machine of claim 18, wherein the functional filter is disposed between the ion exchange resin filter and the water outlet. 19. The water softener or skin care device of claim 18, wherein the functional filter is mounted in an ion exchange resin filter. The water softener or skin care machine of claim 18, wherein the functional filter is disposed separately from the ion exchange resin filter.

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