KR20130117158A - Composition for antioxidant comprising extract of lagerstroemia ovalifolia - Google Patents
Composition for antioxidant comprising extract of lagerstroemia ovalifolia Download PDFInfo
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- KR20130117158A KR20130117158A KR1020120040007A KR20120040007A KR20130117158A KR 20130117158 A KR20130117158 A KR 20130117158A KR 1020120040007 A KR1020120040007 A KR 1020120040007A KR 20120040007 A KR20120040007 A KR 20120040007A KR 20130117158 A KR20130117158 A KR 20130117158A
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- extract
- antioxidant
- fraction
- composition
- obarifolia
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- 239000008158 vegetable oil Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- QUEDXNHFTDJVIY-UHFFFAOYSA-N γ-tocopherol Chemical class OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2200/00—Function of food ingredients
- A23V2200/02—Antioxidant
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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Abstract
Description
The present invention relates to an antioxidant composition comprising a Lastrostromia obarifolia extract, and more particularly, the present invention relates to an antioxidant composition comprising a Lastrostromia obarifolia extract or fractions thereof, health functional food for antioxidants and cosmetics for antioxidants It relates to a composition.
Free radicals that cause aging and disease in the human body, also called free radicals, are formed by biological reactions during normal metabolic processes in the human body, and are known to cause various diseases by causing oxidative stress on cells and tissues. To date, it is known that digestive diseases, diabetes, cancer, cardiovascular diseases, degenerative diseases and the like are caused by oxidative stress. In order to prevent this, as a physiological action for inhibiting the production of free radicals in a living body, a mechanism of action is provided by donating electrons to oxidative free radicals to inhibit oxidation or to convert superoxide anion radicals into normal oxygen. This is known.
The peroxide radical is known to be decomposed into hydrogen peroxide and water by an enzyme called superoxide dismutase or to react with nitric oxide to generate peroxy nitrite. The hydrogen peroxide combines with metal ions such as iron or copper ions to produce hydroxy radicals, which are powerful active oxygen, which are known to promote aging of cells. In addition, the peroxide is known to cause oxidation of SH-group, nitrification of protein tyrosine, lipid peroxidation, DNA damage and the like. Since the activity of superoxide dismutase is maintained at a high level in the body, the amount of hydrogen peroxide generated is maintained at a higher level than peroxide, but since hydrogen peroxide is decomposed into water and carbon dioxide by catalase or glutathione peroxidase, It is known that only about 5% of the level remains. However, since the remaining 5% hydrogen peroxide generates hydroxy radicals, these residual hydrogen peroxides have to be removed, and an antioxidant material is used for this purpose.
Antioxidants are widely distributed in nature, and mainly phenolic compounds derived from plants, flavonoids, tocopherols, vitamin C, selenium, etc. are used as antioxidants and are used as raw materials for food, medicine, and cosmetics. . However, since natural antioxidants derived from nature do not have a large antioxidant effect, they must be used in large quantities in order to obtain effectiveness using these antioxidants. there was.
In order to solve these problems of natural antioxidants, there is an active progress to develop chemically synthesized antioxidants. For example, chemically synthesized antioxidants such as Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate have been developed. These synthesized antioxidants have superior antioxidant effects than natural antioxidants. Therefore, it is recognized as a means to solve the problem of natural antioxidants. However, these synthesized antioxidants have a new problem of causing large and small side effects in the human body, and their use is limited.
Under these backgrounds, the present inventors have made intensive studies to develop an antioxidant substance which is derived from natural products and ensures safety while exhibiting excellent antioxidant effect. As a result, the extract derived from the natural product, Lastrostromia obarifolia, has an excellent antioxidant effect. It confirmed that it shows and completed this invention.
It is an object of the present invention to provide an antioxidant composition comprising a Rogerstromia obarifolia extract or a fraction thereof.
It is another object of the present invention to provide a dietary supplement for antioxidants comprising Rogersstromia obarifolia extract or fractions thereof.
Still another object of the present invention is to provide an antioxidant cosmetic composition comprising an extract of Lastrostromia obarifolia or a fraction thereof.
As one embodiment for achieving the above object, the present invention is Lagerstroemia ovalifolia ) provides an antioxidant composition comprising an extract or a fraction thereof.
As used herein, the term "Lagerstroemia ovalifolia" refers to a kind of evergreen shrub belonging to the genus Lagerstroemia native to India, Indonesia, Northern Australia, etc., but the specific bioactive substance or The effect is not yet identified. The inventors have found that the extracts derived from Rastrostromia obarifolia are free radicals derived from DPPH (1,1-diphenyl-2-picryl-hydrazyl) and ABTS (2,2'-azino-bis (3-ethylbenzthiazoline- 6-sulfonic acid)) by demonstrating the ability to eliminate free radicals derived from and peroxide radicals produced by SOD, the extract first demonstrated excellent antioxidant activity.
As used herein, the term "Lastrostromia obarifolia extract" refers to an extract obtained by extracting Lastrostromia obarifolia, for the purposes of the present invention, Lastrostromia obarifolia extract It may be a result obtained by extracting barifolia or a pulverized product thereof with water, C 1 to C 4 alcohols (methanol, ethanol, butanol, and the like) or a mixed solvent thereof, and preferably, Rogerstromia obarifolia Or it may be a result obtained by extracting the pulverized product with methanol, but is not limited thereto, and the result is an extract, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and a crude or purified thereof It may include any one or more. The extract may be obtained using conventional extraction methods in the art, such as ultrasonic extraction, filtration and reflux extraction, and the used Rogerstromia obarifolia may be purchased and used commercially, or collected or grown in nature. May be used, but is not particularly limited thereto.
The term "fraction" of the present invention means the result obtained by the fractionation method of separating a specific component or a specific group from a mixture comprising various components. For the purposes of the present invention, the fraction is preferably fractionated by the solvent fractionation method using an organic solvent such as DMSO, n-hexane, ethanol, ethyl acetate, butanol, a mixed solvent thereof, extracts of the Lastrostromia obarifolia It may be a result, and may include both a polar solvent fraction and a nonpolar solvent fraction, DMSO fraction, n-hexane fraction, ethanol fraction, ethyl acetate fraction, butanol fraction, mixed solvent fractions, etc. may be used, but It is not particularly limited to this.
The term "antioxidant" of the present invention includes, in a narrow range, the action of inhibiting, reducing or controlling the production or reaction of free radicals produced in the body, hydrogen peroxide or peroxide produced from the free radicals, and hydroxy radicals produced from the hydrogen peroxide. In a broad range, it means an action of inhibiting, reducing or controlling the generation of an oxidation reaction occurring in nature. For the purposes of the present invention, the antioxidant is understood as a narrow range of antioxidants, and may be understood as an action of inhibiting, reducing or controlling the production or reaction of free radicals or hydrogen peroxide, which occur mainly at the cellular level, but is not particularly limited thereto. Do not.
On the other hand, the composition of the present invention may further comprise at least one active ingredient exhibiting the same or similar function in addition to the extract of Lastrostromia obarifolia.
In addition, the composition of the present invention may be a pharmaceutical composition further comprising a pharmaceutically acceptable carrier. The composition comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose ( lactose), gelatin and the like can be mixed. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups. In addition to the commonly used simple diluents, water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like. The pharmaceutical composition may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations and suppositories It can have one formulation.
In addition, the route of administration of the composition of the present invention may be administered through any general route as long as it can reach the target tissue, and as desired, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration. , Oral administration, nasal administration, pulmonary administration, rectal administration, but is not limited thereto. In addition, the composition may be used alone or in combination with hormone therapy, drug therapy and biological response modifiers to exhibit an antioxidant effect.
In another aspect, the composition of the present invention may be administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount" of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is determined by the kind and severity of the subject, age, sex, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts. The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And single or multiple administrations. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by a person skilled in the art.
The composition of the present invention may be used alone or in combination with methods using surgery, hormonal therapy, drug therapy and biological response modifiers for the prevention or treatment of inflammatory diseases.
According to one embodiment of the present invention, the free radical scavenging activity of the sample obtained by dissolving methanol extract of Lagerstromia obarifolia in DMSO was measured. DPPH (1,1-diphenyl-2-picryl-hydrazyl) In addition to the concentration-dependent scavenging of free radicals derived from, the scavenging activity is dependent on free radicals derived from ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)). It was confirmed that it was shown (Fig. 1 and 2). In addition, it inhibited the activity-dependent concentration of SOD (superoxide dismutase enzyme) producing peroxide radicals, it was confirmed to exhibit an antioxidant effect superior to the synthetic antioxidant BHA (Fig. 3).
As another aspect, the present invention provides a dietary supplement for antioxidants comprising Rogersstromia obarifolia extract or fractions thereof.
The term "health functional food" of the present invention is the same term as food for special health use (Food for special health use, FoSHU), the food processed with high medical effect, medical treatment so that the bioregulatory function is effectively displayed in addition to nutrition In some cases, it may be used interchangeably with terms such as health food, functional food, health supplement food. For the purposes of the present invention, the health functional food may be prepared to obtain an antioxidant activity to obtain a useful effect in maintaining, improving or restoring the health of the individual ingesting the same, the product form thereof is preferably a tablet, capsule, powder It may be prepared in various forms such as granules, liquids, and rings, but is not particularly limited now.
When using the Rogerstromia obarifolia extract as a food additive, the extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment).
There is no particular limitation on the kind of the food. Examples of foods to which the Rogerstromia obarifolia extract can be added include meats, sausages, breads, chocolates, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice creams including ice cream, various soups and drinks , Teas, drinks, alcoholic beverages and vitamin complexes and the like, and includes all of the health foods in the conventional sense.
In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food of the present invention may contain natural fruit juice, fruit juice beverage and flesh for the production of vegetable beverages.
As another aspect, the present invention provides a cosmetic composition for antioxidant comprising a Lagerstromia obarifolia extract or a fraction thereof.
According to one preferred embodiment, the cosmetic composition of the present invention can be used for the production of functional cosmetics.
The term "cosmedical, cosmeceutical" of the present invention refers to a product having a professional function that has a physiologically active effect and an effect emphasized, unlike general cosmetics, A product that helps, a product that helps to improve wrinkles, a product that helps protect skin from burns or UV rays. Functional cosmetics for the purpose of the present invention refers to a product that adds an antioxidant effect to the skin cells from various functional cosmetics, preferably functional cosmetics for antioxidants comprising an extract or fractions of Lastrostromia obarifolia as an active ingredient This may be, but is not particularly limited thereto, and the content of the Rasstromia obarifolia extract or fractions thereof included in the antioxidant functional cosmetics is not particularly limited.
The functional cosmetics of the present invention can be prepared in any of the formulations commonly prepared in the art, preferably solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, etc. More specifically, flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, lotion, gel, essence, eye cream, cleansing It may be prepared in the form of a cream, cleansing foam, cleansing water, pack, ointment, stick, patch, spray or powder, but is not limited thereto.
The antioxidant composition of the present invention, including the extract derived from the natural product, Rogerstromia obarifolia, while ensuring safety, it is superior to the conventional natural antioxidants, and has an antioxidant effect equivalent to that of synthetic antioxidants. Therefore, the present invention may be widely used for the development of various products including antioxidants.
1 is a graph showing the scavenging activity of free radicals derived from DPPH according to the concentration of extract of Lastrostromia obarifolia, BHA means a group treated with BHA at 10 μg / ml.
FIG. 2 is a graph showing the scavenging activity of free radicals derived from ABTS according to the extract concentration of Rasstromia obarifolia, and BHA refers to a group treated with BHA at 10 µg / ml.
Figure 3 is a graph showing the SOD inhibitory activity according to the extract concentration of Lagerstromia obarifolia, BHA means a group treated with BHA 10 ㎍ / ㎖.
Figure 4 is a schematic diagram showing a process for preparing a solvent-specific fractions in the methanol extract of Lagerstromia obarifolia.
Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.
Preparation Example: Preparation of Rogerstromia obarifolia extract and fractions
Lagerstroemia ovalifolia ) extract, a methanol extract, was purchased from the Overseas Biomaterials Hub Center of Korea Research Institute of Bioscience and Biotechnology. The dried extract was dissolved in DMSO solvent at a concentration of 20 mg / ml and used for each experiment below (hereinafter referred to as "Lastrostromia obarifolia extract").
In addition, the fraction of Lastrostromia obarifolia was suspended by adding 10 ml of water to 98 mg of methanol extract, followed by shaking three times with the same amount of 100% hexane to obtain 14.4 mg of the hexane fraction. Then, the hexane fraction was removed, and the same amount of chloroform was added to the remaining water layer, which was then left to shake three times to obtain 17.2 mg of chloroform fraction. The same amount of ethyl acetate was added to the remaining water layer to give 51.3 mg of ethyl acetate fraction in the same manner. In addition, the same amount of butanol was added to the remaining water layer to obtain 5 mg of butanol fraction in the same manner. The remaining water layer was concentrated, but no water fraction was obtained (FIG. 4). Each fraction was dried and dissolved in DMSO solvent at a concentration of 20 mg / ml and used for the following experiment.
Experimental Example 1 Evaluation of DPPH Free Radical Scavenging Activity of Rasstromia obarifolia Extract
Free radical scavenging activity derived from DPPH (1,1-diphenyl-2-picryl-hydrazyl) was measured using a fraction of the methanol extract of Lagerstromia obarifolia.
Specifically, 90 μl of a solution in which DPPH was dissolved in ethanol at a concentration of 0.15 mM was dispensed into a 96-well plate, and 10, 20, 40, 80, or 160 μg of the Rogerstromia obarifolia extract solution prepared in Preparation Example, respectively. After diluting to a concentration of / ml and adding thereto, and reacting at room temperature for 20 minutes, the absorbance was measured at 517 nm. The measured absorbance was applied to Equation 1 below to calculate free radical scavenging activity, and compared with each other (FIG. 1). At this time, the experimental group treated with DMSO alone was used as a negative control group, and the group treated with BHA, a synthetic antioxidant, was used as a positive control group.
A; Absorbance of Negative Controls Not Treated with Extracts of the Present Invention
B; Absorbance of the experimental group treated with the extract of the present invention
1 is a graph showing the scavenging activity of free radicals derived from DPPH according to the extract concentration of Lagerstromia obarifolia. As shown in Figure 1, it was confirmed that the scavenging activity of free radicals was increased in proportion to the extract concentration of Lagerstromia obarifolia.
Experimental Example
2:
Rogers Tromia
Obaripolia
Extract
ABTS
Free radical
Scavenging activity assessment
Fractions of the methanol extract of Lagerstromia obarifolia were used to determine free radical scavenging activity derived from ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)).
Specifically, the final concentrations of ABTS and potassium persulfate were mixed to 7 mM and 2.45 mM, respectively, and reacted for 12 hours in the dark to form ABTS radicals. Distilled water was added to the reaction to dilute the absorbance to 0.7 at 734 nm, and the diluted reaction was dispensed into a 96 well plate. The extract solution prepared in Preparation Example was diluted to 10, 20, 40, 80 or 160 µg / ml, respectively, and added thereto, and then reacted for 7 minutes at room temperature in the dark, and then absorbance was measured at 734 nm. The measured absorbance was applied to Equation 1 to calculate free radical scavenging activity, and compared with each other (FIG. 2). At this time, the experimental group treated with DMSO alone was used as a negative control group, and the group treated with BHA, a synthetic antioxidant, was used as a positive control group. FIG. 2 is a graph showing the scavenging activity of free radicals derived from ABTS according to the extract concentration of Lagerstromia obarifolia. As shown in Figure 2, it was confirmed that the scavenging activity of free radicals was increased in proportion to the extract concentration of Lagerstromia obarifolia.
Experimental Example
3:
Diphterocarpus
Opportunity
Extract
SOD
activation
In order to confirm the antioxidant activity of the extract of the preparation, the scavenging activity of the superoxide radical acting as a precursor of other harmful active oxygen species was measured. The superoxide radical scavenging activity can be detected by measuring the activity of superoxide dismutase (SOD), an enzyme that eliminates superoxide by dismutating the superoxide. Was used. Specifically, a sample of each concentration (10, 20, 40, 80 or 160 µg / ml) was dispensed into a 96-well plate and water-soluble WST-1 (2- (4-indophenyl) -3- reacted with a superoxide radical. After (4-nitrophenyl) -5- (2,4-disulfophenyl) -2H-tetrazolium) was added, the SOD enzyme solution was added and reacted at 37 degrees for 20 minutes, and then the absorbance of the reaction solution was microplate. It measured at 450 nm with the measuring instrument. The superoxide radical scavenging activity of each sample was calculated as the degree of reduction of the reduction of WST relative to the control. At this time, the group treated with DMSO was used as a negative control, and the group treated with BHA as a synthetic antioxidant was used as a positive control.
As a result, as shown in Figure 3 it was confirmed that the superoxide radical scavenging ability of the Rogerstromia obarifolia extract concentration-dependent. The superoxide radical scavenging ability showed 34.79 ± 2.70% (BHA) and 47.92 ± 0.39% (extract), respectively, when BHA and extracts were treated at a concentration of 10 µg / ml, indicating that the extract of superstratum obarifolia It was confirmed that the radical scavenging activity showed significantly higher activity than the positive control group.
Experimental Example
4:
Rogers Tromia
Obaripolia
Fraction
Free radical
Scavenging activity assessment
In order to confirm the antioxidant activity of the fractions of the preparation, DPPH and ABTS free radical scavenging ability of each fraction was measured by the methods of Experimental Examples 1 and 2.
In addition, because Lastrostromia obarifolia is a natural product, natural antioxidants, vitamin E and vitamin C, were used as positive controls, and the samples were treated at a concentration of 40 μg / ml.
Hexane layer
Chloroform layer
Ethyl acetate layer
Butanol layer
Vitamin E
40
40
40
40
40
40
7.27 ± 1.00
9.22 ± 1.20
15.05 ± 0.70
30.36 ± 0.73
38.50 ± 2.20
57.55 ± 1.90
11.16 ± 0.09
18.34 ± 1.19
25.32 ± 0.45
38.20 ± 0.64
52.36 ± 6.64
100.10 ± 0.09
As a result, as shown in Table 1, the radical scavenging ability of Rogerstromia obarifolia methanol extract was 24.22 ± 1.05% (DPPH) and 34.07 ± 0.15% (ABTS), respectively, but the radical scavenging ability of the butanol layer fractionated therefrom was respectively 30.36 ± 0.73% (DPPH) and 38.20 ± 0.64% (ABTS) showed higher antioxidant activity.
Claims (6)
The extract is a composition that is extracted by using a lower stromymia obarifolia or a pulverized water, an alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.
The fraction is obtained by fractionating the extract with a solvent selected from the group consisting of DMSO, n-hexane, ethanol, ethyl acetate, butanol and mixed solvents thereof.
Antioxidant which is included in a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, lotion, gel, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, ointment, stick, patch, spray or powder Cosmetic composition for.
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| CN110477253A (en) * | 2019-08-20 | 2019-11-22 | 苏州科技大学 | Crape myrtle fruit Substance extraction process and application |
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| CN110477253A (en) * | 2019-08-20 | 2019-11-22 | 苏州科技大学 | Crape myrtle fruit Substance extraction process and application |
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