KR20130051543A - Manufacturing method of composition of cosmetic using plant extract from codonopsis lanceolata and the composition of cosmetic - Google Patents

Abstract

PURPOSE: A method for preparing a cosmetic composition for improving the skin condition using extracts of mulberry leaves, stems, root barks, and fruits is provided to improve skin whitening, moisturizing, and anti-wrinkling effects. CONSTITUTION: A method for preparing a cosmetic composition for improving skin condition using mulberry leaves, stems, root barks, and fruits comprises: a step of drying and pulverizing the mulberry leaves, stems, root barks, and fruits; a step of inoculating 5-20wt% of lactic acid bacteria to 80-95wt% of pulverized mulberry, and fermenting at 28-37 deg. C in 73-75% humidity for 6-8 days; a step of maturing at 18-22 deg. C for 5-8 days for preparing the fermented mulberry; a step of drying the fermented mulberry; a step of adding the fermented mulberry to a solvent, and extracting at 65-75 deg. C for 6 hours to prepare an extract; and a step of adding the extract to an emulsifier base.

Description

Manufacturing method of cosmetic composition using the extract of the leaves, stems, root bark, berries of the mulberry and cosmetic composition prepared therefrom {Manufacturing method of composition of cosmetic using plant extract from codonopsis lanceolata and the composition of cosmetic}

The present invention relates to a method for preparing a cosmetic composition using extracts of leaves (upper leaves), stems (upper limbs), root bark (upper skin), fruit (audio) of mulberry and cosmetic compositions prepared therefrom.

Mulberry is a genus of rosewood mulberry family. Most of the species are native to Asia. It is genetically close to Broussonetia. Mulberry is a fast-growing tree when young, but grows slowly, reaching 10-15 m in height. Fruits ripen, dark purple to black, can be eaten, and some fruits are very sweet. Root bark is called baekbaekpi, fruit is mulberry (mugi), leaves are upper leaves, branches are called upper limbs.

Sangbaikpi (Cortex Mori: mulberry tree root bark) is the root bark of Morus bombycis koidzumi, Morus alba Linne or other similar plants belonging to the Moraceae family.

Audi is a mulberry fruit, which contains glucose, fructose, calcium, iron, potassium, magnesium, zinc, vitamins A, B1 and C.

Audi contains four to five times more iron than other fruits, two times more calcium than strawberries, two times more potassium to apples, and more magnesium and zinc.

Neohesperidine is a powerful antioxidant that is known to prevent skin lipids and oxidation, strengthening skin tissues and increasing skin elasticity. AHA9 alpha hydroxy acid regulates abnormal skin angles to maintain clean skin at all times. There is a function to let it.

A substance that enhances or inhibits the function of a living body in living life, and is a substance that corrects abnormal conditions caused by a lack or excessive secretion of a substance involved in function regulation in a living body. This can be defined. The creation of bioactive substances is of great importance for a better and healthy life of the living body. The creation of new bioactive substances can be obtained from natural products, such as plants and animals, or can be extracted and purified from microorganisms and metabolites of animal and plant cell lines, or by chemical synthesis.

Currently, mulberry leaves are part of the mulberry tree is used for slumber, and the fruit of the mulberry is sold for some food. On the other hand, the present inventors prepared the extract by using the whole parts of the mulberry tree, baekbaekpi (root bark), Audi (fruit), upper leaf (leaves), upper limbs (branch), so that the extracted extract can be used in cosmetics This has led to the completion of the present invention in order to be able to create a lot of added value in the rural economy.

The present invention is to prepare a cosmetic composition using the extract obtained from the leaves, stems, roots, berries of the mulberry, confirming that the mulberry can be used as a cosmetic material, excellent physiological activity effect, whitening, moisturizing, wrinkle improvement, etc. Providing a cosmetic composition excellent in the skin improvement effect of the skin improvement cosmetic composition manufacturing method using the extract of leaves, stems, roots, berries of mulberry trees that can create a lot of added value to the rural economy and the cosmetic composition prepared therefrom What is intended is for the purpose of the invention.

In order to achieve the above object,

The present invention is inoculated with lactic acid bacteria 5 ~ 20wt% to 80 ~ 95wt% of mulberry pulverized after drying the leaves, stems, root bark, fruit of mulberry 6 ~ 28 ~ 37 ℃, humidity 73 ~ 75% conditions Fermentation for 8 days,

To obtain a mulberry fermented product by aging the fermented mulberry crushed product at 18 ~ 22 ℃ for 5 to 8 days,

Drying the obtained mulberry fermented product in a dryer;

Adding the dried mulberry fermented product to a solvent, adding 7 to 13% of the total weight of the solvent to extract the extract for 6 hours at 65 to 75 ° C. to prepare an extract;

The extract is added to the cosmetic emulsion base, the skin using the extract of leaves, stems, root bark, fruit of mulberry tree comprising the step of preparing a cosmetic by adding 4 ~ 10wt% to the total weight of the cosmetic emulsion base A method for producing an improved cosmetic composition and a cosmetic composition prepared therefrom are the main technical configurations.

In addition, the lactic acid bacteria is Leuconostoc mesenteroides KCCM 35471, characterized in that the solvent is water or ethanol.

The cosmetic composition according to the present invention has the advantage of providing cosmetics with excellent skin improvement effects such as whitening, moisturizing, wrinkle improvement, etc. by using the extract contained in the leaves, stems, roots, berries of mulberry.

Hereinafter, the skin improvement cosmetic composition manufacturing process using the extract of leaves, stems, root bark, fruit of the mulberry tree according to the present invention.

Fermentation and Extraction of Mulberry Leaves, Stems, Root Barks, Berries

As described above, the extract of the leaves, stems, root bark, and fruit of the mulberry is a mulberry pulverized pulverized leaf (upper leaf), stem (upper limb), root bark (allium skin), fruit (audio), which are the ingredients of the mulberry tree. After inoculating 5 ~ 20wt% lactic acid bacteria solution for 80 ~ 95wt%, after 6 to 8 days of fermentation under the conditions of 28 ~ 37 ℃, humidity 73 ~ 75% is prepared through the extraction process.

When the amount of the mulberry pulverized product is less than 80wt% or more than 95wt%, the fermentation by lactic acid bacteria may not be sufficient, so the functionality of the skin-improving cosmetics may fall, so the content of mulberry crushed products is within the range of 80 to 95wt%. It is preferable to limit.

The lactic acid bacterium is Leuconostoc mesenteroides (Leuconostoc mesenteroides KCCM 35471), when the amount is less than 5wt% fermentation is not made smoothly, the functionality of the moisturizing cosmetics may be lowered, if lactic acid bacteria exceed 20wt% Since the amount of use is meaningless because the excess no longer affects the fermentation, it is preferable to limit the amount of lactic acid bacteria inoculation within the range of 5 to 20wt%.

And, as the fermentation conditions, if less than 28 ℃, or more than 37 ℃ when the activity of the lactic acid bacteria is not properly made, there is a problem that the fermentation is not made smoothly, the humidity is less than 73%, or exceeds 75% Even if the fermentation may not be made properly, it is preferable to maintain the conditions of temperature and humidity of 28 ~ 37 ℃, humidity 73 ~ 75% conditions.

In addition, if the fermentation period is less than 6 days, it is too early to finish fermentation because it is not yet fully fermented, and if it exceeds 8 days, the fermentation period is already sufficient. Since it is meaningless to maintain, the fermentation period is preferably maintained within the range of 6 days to 8 days.

As such, the fermented product is subjected to the extraction process after drying in a dryer.

More specifically, we will look at the process of obtaining the leaves, stems, root bark, fruit extract of mulberry.

Manufacture of Mulberry Leaves, Stems, Root Bark, Fruit Extract

Leaves, stems, roots, and berries of each part of mulberry tree (provided by Hongseong Mulberry Land, a farming method) are dried and pulverized. Fermented product is obtained by fermentation for 5 days at 36 ° C. and 70% humidity, and then aged at 20 ° C. for 7 days after inoculation.

Next, the fermented product was dried in a drier, and the fermented product was contained in 10% of water or ethanol solvent and extracted at 70 ° C. for 6 hours to obtain an extract.

In this case, in the case of extraction with water, 10 times of water was added to the fermented product, and hot water was extracted at 70 ° C. for 6 hours, followed by filtration with filter paper to obtain a hot water extract.

In the case of extraction using ethanol, 10 times of ethanol was added to the fermented product, followed by ethanol extraction at 70 ° C. for 6 hours, followed by filtration with filter paper to obtain an ethanol extract.

Evaluation of safety and effectiveness of extract

Experiments were conducted to evaluate the safety and effectiveness of extracts using hot water extracts. The test period was from April 6 to 13, 2011, and 17 men and women in their 20s were selected.

In order to evaluate the safety and efficacy of the extract, CK MPA 5 was used, the sediment was measured using Sebumeter (SM815), the moisture was measured by Corneometer (CM825), and the water evaporation was measured by Tewameter (TM300) and melanin pigment. Mexameter (MX18) was used for measurement, and skin elasticity measurement was performed using Reviscometer (RVM600).

Measurements were averaged after 3 measurements, and the general skin type of the subjects was analyzed based on the measured data. The measurement results are shown in Table 1.

Skin measurement result No. name gender age location Oil content Moisture content Melanin levels skin
pH Skin moisture
Evaporation One
this**
south
21
T-zone 49 56.40 176.50 6.68 8.80 U-zone 150 44.00 278.50 4.87 15.50 2
Yoon **
south
21
T-zone 225 47.90 215.00 6.25 12.90 U-zone 225 42.00 244.00 5.95 41.55 3
Kim**
south
23
T-zone 220 36.30 166.50 6.60 12.60 U-zone 76 51.90 241.00 5.90 15.90 4
foil**
south
23
T-zone 190 52.25 222.50 5.50 9.10 U-zone 171 53.10 236.50 4.40 18.55 5
this**
south
26
T-zone 204 43.15 174.50 5.52 14.55 U-zone 211 44.50 226.50 5.86 14.20 6
article**
south
26
T-zone 64 33.00 145.00 5.30 13.00 U-zone 239 39.00 245.00 5.25 9.50 7
Yoon **
female
20
T-zone 33 52.20 175.50 5.01 11.05 U-zone 152 50.70 283.00 4.80 8.80 8
God**
female
20
T-zone 22 66.00 131.00 5.58 9.20 U-zone 77 63.50 200.50 5.20 6.70 9
Kim**
female
20
T-zone 26 73.50 122.50 6.54 11.80 U-zone 59 71.15 190.00 5.23 9.50 10
this**
female
21
T-zone 75 59.05 154.00 5.90 14.60 U-zone 100 49.00 281.00 5.90 8.80 11
article**
female
21
T-zone 48 46.40 121.00 6.72 13.60 U-zone 95 146.50 146.50 6.30 10.90 12
Kim**
female
21
T-zone 35 57.45 126.50 6.80 10.30 U-zone 44 54.70 161.00 6.30 11.50 13
Choi **
female
22
T-zone 3 43.05 148.50 5.90 11.45 U-zone 23 40.00 215.00 5.80 28.20 14
book**
female
22
T-zone 32 26.00 135.00 5.80 14.00 U-zone 75 39.00 255.00 5.25 8.40 15
I'm**
female
20
T-zone 24 39.53 113.00 5.42 13.90 U-zone 45 51.83 202.66 5.20 14.86 16
Choi **
female
20
T-zone 26 43.76 160.66 5.08 8.10 U-zone 52 39.20 196.33 5.10 11.16 17
book**
female
20
T-zone 14 28.03 118.00 5.97 10.40 U-zone 171 35.23 192.66 5.86 13.60

Cosmetic formulation  Application evaluation

Epidermal hot water extract (A), hot leaf hot water extract (B), hot water hot water extract (C), Audi hot water extract (D) was added to the cosmetic emulsion base to test the skin improvement effect when applying the cosmetic formulation. Cosmetic formulations used for formulation evaluation are shown in Table 2 below.

Cosmetic formulation Raw material name Control
(Comparative Example) A
(Example) B
(Example) C
(Example) D
(Example)


Paid




Butlyparaben 0.15 0.15 0.15 0.15 0.15 Ethylhexyl Methoxycinnamate 0.20 0.20 0.20 0.20 0.20 Cetyl Alchohol 0.80 0.80 0.80 0.80 0.80 Glyceryl Stearate, PEG-100 Stearate 2.00 2.00 2.00 2.00 2.00 Sorbitan Stearate 1.00 1.00 1.00 1.00 1.00 Meadowfoam Seed oil 2.00 2.00 2.00 2.00 2.00 Vitamine E Acetate 0.10 0.10 0.10 0.10 0.10 Cyclopentasiloxane 2.00 2.00 2.00 2.00 2.00 Triethylhexanoin 4.00 4.00 4.00 4.00 4.00 Dimethicone200F 1.00 1.00 1.00 1.00 1.00


Awards

Methylparaben 0.15 0.15 0.15 0.15 0.15 Triethanolamine 0.10 0.10 0.10 0.10 0.10 Glycerin 2.00 2.00 2.00 2.00 2.00 EDTA 2Na (Ethylenediaminetetraacetic Acid, disodium Salt) 0.01 0.01 0.01 0.01 0.01 Propylene glycol 4.00 4.00 4.00 4.00 4.00 D.I.Water 73.20 68.2 68.2 68.2 68.2
Incrementer Carbomer 0.14 0.14 0.14 0.14 0.14 D.I.Water 7.00 7.00 7.00 7.00 7.00


extract Morus Extract Hot Water Extract - 5.00 - - - Upper lobe hydrothermal extract - - 5.00 - - Upper limb hydrothermal extract - - - 5.00 - Audi hydrothermal extract - - - - 5.00 Spices Jasmine Yu 0.15 0.15 0.15 0.15 0.15 Sum 100 100 100 100 100

Each product according to Table 2 was visually inspected in a 45 ° C. incubator for 30 days, and pH and viscosity changes were measured for 30 days at 25 ° C. at room temperature (Table 3 to Table 5).

45 ℃ incubator 1 week 2 weeks 3 weeks 4 weeks Control
(Comparative Example) clear clear clear clear A
(Example) clear clear clear clear B
(Example) clear clear clear clear C
(Example) clear clear clear clear D
(Example) clear clear clear clear

25 ℃ pH  Change constantly pH meter  Model and measurement method) pH 1 week 2 weeks 3 weeks 4 weeks Control
(Comparative Example) 6.55 6.61 6.65 6.50 A
(Example) 6.49 6.39 6.38 6.44 B
(Example) 6.33 6.29 6.24 6.32 C
(Example) 6.46 6.40 6.39 6.48 D
(Example) 6.71 6.65 6.63 6.72

Constant change of viscosity at 25 ℃ Brookfield LV Viscometer No .4 Spindle  6 rpm ) cP 1 week 2 weeks 3 weeks 4 weeks Control
(Comparative Example) 35000 31000 31500 31500 A
(Example) 18000 14000 18500 18500 B
(Example) 18000 16000 13500 14500 C
(Example) 14000 16000 18000 15500 D
(Example) 19000 22000 22000 22000

Skin application evaluation

As a skin irritation test, a skin safety evaluation test was performed on a cosmetic composition to which hot water extract was added. A single patch test was carried out, but 10 subjects were tested inside the upper arm.

Visual evaluation was performed two times 24 hours after the patch and 48 hours after the patch was applied to the inside of the extract 3mg upper arm (inside the arm) by using a patch test device (IQ Ultra).

Skin improvement evaluation

After applying 1 g of the non-coated spot, A ~ D product, and the control product, the skin condition change was measured for 3 hours, and the measurement results are shown in Tables 6 to 10.

Skin improvement result

A (Example) moisture Elasticity Melanin One 45 -16.67 -3.33 2 39.33 -1.66 -43.33 3 35.21 -34.34 -23.33 4 30.1 11.54 -66.66 5 6.33 -28.33 13.34 6 36.33 -22.66 -26.67 7 28 -9 -40 8 32.21 3.33 43.33 9 31 -34.33 -26.67 Sum 283.51 -132.12 -173.32 Average 31.501 -14.68 -19.2578

B (Example) moisture Elasticity Melanin One 44 -20 -3.34 2 41 -2 -33.34 3 35.12 -5 -56.67 4 25.44 37.34 5 15 -11.67 33.33 6 42.33 -31.33 -40 7 20.67 -24.67 -16.67 8 32.45 58.34 -6.66 9 33.66 -12 -26.67 Sum 289.67 -10.99 -150.02 Average 32.18556 -1.2211 -16.6689

C (Example) moisture Elasticity Melanin One 42.34 -14 -3.33 2 43.67 33.4 0 3 32.22 -41.33 -43.33 4 36.87 58 -6.67 5 14.67 49.73 10 6 41.67 -22.67 -6.33 7 18.34 -21.67 -10 8 36.24 32.33 0 9 30.1 -15.33 -30 Sum 296.12 58.46 -89.66 Average 32.9022 6.4956 -9.962

D (Example) moisture Elasticity Melanin One 40.33 -6.33 -23.33 2 18 -17.33 -6.67 3 18.45 -35.34 -43.34 4 20.55 -33.66 -16.67 5 -0.34 -48.67 63.33 6 41.33 -48.33 -19.67 7 4 -155 -20 8 15.22 101.66 -23.34 9 25.43 -29.67 -53.33 Sum 182.97 -272.67 -143.02 Average 20.33 -30.297 -15.8911

control moisture Elasticity Melanin One 21 -8.67 10 2 34 5.33 -16.67 3 22.47 11.33 -23.33 4 28.88 143.34 -16.66 5 13.33 -44 46.66 6 30.27 -9 -10 7 19.34 -42.67 -43.33 8 18.48 35 -20 9 25.42 8.67 6.67 Sum 213.19 99.33 -66.66 Average 23.68778 11.0367 -7.40667

As described above, the skin improvement cosmetic composition manufacturing method using the mulberry according to the present invention and the cosmetic composition prepared therefrom is reduced production cost by simplifying the process for producing the extract from the mulberry, and also extract the cosmetics When added, the skin improvement effect is increased, so the industrial applicability is large.

Claims (4)

After inoculating 5 ~ 20wt% of lactic acid bacteria to 80 ~ 95wt% of mulberry crushed after drying leaf, stem, root bark and fruit of mulberry, fermented for 6-8 days at 28 ~ 37 ℃, 73 ~ 75% humidity To do that,
To obtain a mulberry fermented product by aging the fermented mulberry crushed product at 18 ~ 22 ℃ for 5 to 8 days,
Drying the obtained mulberry fermented product in a dryer;
Adding the dried mulberry fermented product to a solvent, adding 7 to 13% of the total weight of the solvent to extract the extract for 6 hours at 65 to 75 ° C. to prepare an extract,
The extract of the mulberry leaves, stems, roots, berries of the mulberry tree, characterized in that it comprises the step of adding the extract to the cosmetic emulsion base, adding 4 ~ 10wt% to the total weight of the cosmetic emulsion base Skin improvement cosmetic composition manufacturing method using the extract.
The method according to claim 1,
Lactobacillus is a method for producing a skin improvement cosmetic composition using the extract of leaves, stems, roots, berries of mulberry, characterized in that Leukonostoc menteroides (Leuconostoc menteroids KCCM 35471).
The method according to claim 1,
Method for producing a skin improvement cosmetic composition using an extract of leaves, stems, roots, berries of mulberry, characterized in that the solvent is water or ethanol.
Skin improvement cosmetic composition manufacturing method using the extract of leaves, stems, roots, berries of mulberry, characterized in that it is prepared by the method of claim 1.