KR20130031691A

KR20130031691A - The cosmetic composition for promoting desquamation containing the extract of dictyophora echinovolvata

Info

Publication number: KR20130031691A
Application number: KR1020110095407A
Authority: KR
Inventors: 전민현; 서성남; 송요현
Original assignee: 바이오메디코스 주식회사
Priority date: 2011-09-21
Filing date: 2011-09-21
Publication date: 2013-03-29

Abstract

The present invention is a mushroom of Dictyophora It relates to a cosmetic composition for promoting keratin exfoliation containing an extract of echinovolvata as an active ingredient. The cosmetic composition of the present invention can be used to remove the dead skin, freckles and keratin and the like by promoting the exfoliation by using the manganese mushroom extract as an active ingredient, it can provide an excellent cosmetic composition and cream for exfoliation.

Description

The cosmetic composition for promoting desquamation containing the extract of Dictyophora echinovolvata}

The present invention relates to a cosmetic composition having a keratin exfoliation promoting effect.

The skin is like a membrane that surrounds the various organs in the body, covering the entire surface of the body, protecting it from various stimuli of the body, maintaining dynamic equilibrium with organs of the living body, and protecting organs. It is a kind. Generally, the skin is divided into three parts from the upper layer of skin: epidermis, dermis and subcutaneous fat layer.

Of these, the epidermis is subdivided into stratum corneum, granule layer, polar layer, and basal layer. Epidermal cells generated in basal layer rise up to granule layer over 14 days, and keratinahlin in the granule layer starts keratinization. As the body fluids in the cells have already escaped, the cells are flattened, and keratinization proceeds. The nucleus, cytoplasm and organelles of the cells are degenerated and replaced by keratinally, which is converted back into Eleidin and then into keratin, the last step. The life of the cell ends and is pushed up to the stratum corneum.

The stratum corneum consists of differentiated epidermal cells (skin cells) and the interstitial skin lipids that fill between them, preventing the body's substances from going out and protecting the body from external physical, chemical and biological stimuli. Do it. Keratinocytes are connected by a protein called desmosome, and as they rise up the stratum corneum, desmosomes are broken down, weakening the cohesion between keratinocytes and finally keratinocytes are separated from the skin. In normal skin, the stratum corneum consists of 15 to 20 layers, and it takes 15 to 20 days to separate completely.

However, as the skin ages, the epidermis, dermis, and subcutaneous tissue become thinner, the moisture content of the skin decreases drastically, and the skin loses its elasticity due to the change of the extracellular matrix (ECM) component that gives elasticity to the skin. Wrinkles may occur, and the separation of the stratum corneum will be later than normal, resulting in thickening of the stratum corneum (stratum corneum) and loss of skin vitality.

The stratum corneum stratification is mainly due to decreased skin moisturizing ability, decreased production and activity of desmosome degrading enzymes, decreased cell activity, and skin aging, UV exposure, and pollution. Artificially thinning the stratum corneum thickened by these internal and external factors increases the activity or regeneration of living cells under the stratum corneum, resulting in softening of the skin and the effects of wrinkle removal, acne outbreaks, and treatments. There is a lot of research to solve the keratin stratification to the extent that it does not. In particular, hyperkeratosis of the foot causes a lot of discomfort in everyday life by causing cracks in the sole or the heel, so the research to solve this situation is in progress.

To relieve keratinization, physically rub the skin or use chemical dermabrasion. In particular, chemical peels have additional effects such as fine wrinkles, rough skin relief and blemishes. Substances used for chemical peeling include trichloroacetic acid, phenol, and alpha hydroxy acid (AHA). However, the alpha hydroxy acid has a disadvantage of complaining of tingling due to low pH and causing irritation, and thus its use is limited.

Recently, mushrooms have been attracting attention as a new natural material in the cosmetic field, showing various effects in functional cosmetics. As an example, Dictyophora echinovolvata is a natural preservative that has efficacy and safety effects on skin inflammation inhibitory and skin stability (Chun, 2004, KR10-2004-84581A), and is used as a natural antibacterial agent (KR10-2006-). 3508A).

Dictyophora (SPP. ) Is a taxonomic bacterium (Gasteromycetidae). Phallales belongs to the Phallaceae family. The common name of this mushroom is called mantle mushroom in Korea. Recently, the bioactive substance was extracted from mantle mushrooms to reveal its structure, and it was found that ditrophorine could be used as a therapeutic agent for the treatment of central nerve regeneration and dementia disease by NGF (never growth factor). Several bioactive substances have been reported and reported to have antimicrobial properties against pathogenic microorganisms (Kawagishi et al. 1997). Moreover, as a pharmacological effect, it is mint. Interpolation. Psoriasis (建腦). Nominal. It is said that there is a painful action (止痛作用) (孫 .1991). Mango mushroom contains 20,2% of crude protein. Crude fat 2.6%. Not only does it contain 38.1% carbohydrates, but it also contains several amino acids, and manta mushrooms have high blood pressure. The treatment effect is very good for patients with high content of cholesterol. As the effects of manta mushrooms are known, the fields of use that have been limited to food or herbal ingredients are being extended to alternative medicine.

The present inventors have completed the present invention by finding a plant extract exhibiting excellent exfoliation effect during the study on the cosmetic composition showing a more efficient keratin effect.

Korean Patent Publication No. 2006-3508, Abstract and Claims Korean Patent Publication No. 2004-84581, Abstract and Claims

It is an object of the present invention to provide a cosmetic composition for promoting keratin exfoliation comprising the extract of manta mushroom as an active ingredient.

Still another object of the present invention is to provide a cosmetic composition for exfoliation power generation comprising the extract of manta mushroom as an active ingredient.

In order to achieve the above object, one embodiment of the present invention provides a cosmetic composition for promoting keratin exfoliation comprising a mantle mushroom extract as an active ingredient.

As used herein, the term "keratin tissue" refers to a keratin-containing layer disposed as the outermost protective sheath of a mammal, including but not limited to skin, hair, toenails, nails, epidermis, hooves and the like.

As used herein, the term "dermatologically acceptable" means that the compositions or components described are suitable for use in contact with human keratinous tissue without excessive toxicity, incompatibility, instability, allergic reactions, etc. .

As used herein, the term “safe and effective amount” is intended to significantly induce positive effects, preferably positive keratinous tissue appearance or tactile effects, including the effects disclosed herein, either independently or in combination. Is sufficient, but low enough to avoid serious side effects.

As used herein, the term "peel, peel and / or replace" means the removal of the upper layer of the stratum corneum (including the hard layer).

The extract can be prepared using conventional methods known in the art, i.e., under conventional temperature and pressure conditions, using conventional solvents. Extraction solvents that can be used in the preparation of the extracts of the mantle mushroom of the present invention include, for example, extraction solvents such as water, lower alcohols having ₁ to ₃ carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol. It can be used alone or in combination. A suitable amount of the extracting solvent is about 1 to 15 times the dry weight of the nettle mushroom extract, may be 5 to 12 times, and may be 10 times, but is not limited thereto. In addition, as the extraction method, heat extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used, and may be extracted and used once or repeatedly. Further, the extraction temperature is not particularly limited as long as the extraction temperature is such that the effective activity of the useful component of the extract of the mandarin mushroom is not removed.

The mushroom extract may be a mycelium or fruiting body extract alone, it may be an extract extracted by mixing the mycelium and fruiting body. The mycelium extract and fruiting body extract may be contained in a weight ratio of 1 to 10: 1 to 10, or a weight ratio of 2 to 8: 2 to 8, or a weight ratio of 4 to 6: 4 to 6. However, the content ratio may be mixed in various weight ratios according to the characteristics of the product.

In addition, one embodiment of the present invention provides a cosmetic composition for promoting keratin exfoliation further comprising keratin degrading enzyme in the extract of manta mushroom.

The mushroom extract and keratin degrading enzyme may be included in a weight ratio of 1 to 10: 0.1 to 1, but is not limited thereto. The content ratio may be mixed in various weight ratios according to the characteristics of the product.

In addition, one embodiment of the present invention provides a cosmetic for promoting keratin exfoliation containing a mushroom extract. The cosmetic composition according to the present invention may contain 0.1 to 20% by weight of the mixed extract in a total weight ratio, may contain 5 to 15% by weight.

The cosmetic composition according to the present invention may include, in addition to the extract, conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and flavorings, and may be prepared in any formulation commonly prepared in the art. Can be. Acceptable "carriers" in cosmetic preparations are compounds or compositions already known and used that may be included in cosmetic preparations, or compounds or compositions that will be developed in the future, that are not toxic, unstable or irritating beyond contact with the human body upon contact with the skin. Say. The carrier may be included in the cosmetic composition of the present invention in an amount from about 1% to about 99.99% by weight, preferably from about 90% to about 99.99% by weight of the composition. However, since the above ratio depends on the above-described formulation in which the cosmetic composition of the present invention is prepared, and on the specific application site (face, neck, etc.) thereof or the preferable application amount thereof, And should not be construed as limiting the scope of the invention.

For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, It is not limited. More specifically, it may be prepared in the form of a flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, hair tonic, shampoo, rinse, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component . In addition, the cream may be produced as a cream for exfoliation for power generation.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Castle cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition comprising the extract of the manure mushroom as an active ingredient is excellent in exfoliation effect, and is effective in removing blemishes such as blemishes, blotch, and freckles. In particular, when used in combination with keratin degrading enzymes can be usefully used as an exfoliating cosmetic for power generation.

Figure 1 is a graph of the exfoliation effect of each of the fruiting body extract and mycelium extract of the mantle mushroom.

Hereinafter, the present invention will be described in more detail based on the following Examples and Test Examples, but the present invention is not limited to the following Examples and Test Examples.

< Example 1> Mushrooms Preparation of extract

The mantle mushroom used in the present invention is a mantle mushroom artificially cultivated in the genus ASI 32001 among preserved strains of the applied microorganisms of the orchards. The liquid cultured mycelium was used as a sample obtained by incubating for 30 days in a 10-ml liquid seed culture bottle using a potato malt extract broth (PMB) medium, followed by filtration and lyophilization.

Preparation of ethanol extract from mantle mushrooms was lyophilized until the fruit body of the mantle mushroom was completely dried, finely crushed and immersed in ethanol at room temperature for 48 hours, filtered through two layers of filter paper (ø 110mm, Whatmam), and then Concentration under reduced pressure afforded an ethanol concentrate. The yield of ethanol extract was 27.4% in fruiting body. Yield was 7.5% mycelium

division Dry weight (g) Ethanol Extract (g) yield(%) mycelium 25.1 1.9 7.5 Fruiting body (mushroom) 29.9 8.2 27.4

< Experimental Example 1> skin keratin turnover measurement

In order to determine the extent of the skin keratin turnover promoting effect by the mushroom extract, the effect of keratin exfoliation was measured in 20 healthy adult men and women between 20-40 years old with respect to the extract of Example 1. Before applying the sample, the inner color of the upper arm of the test subject was measured with a chromameter (CR300, Minolta, Japan), and 10% by weight of dihydroxyacetone (DHA) was prepared using a hill top chamber (The Hill Top). ) Into about 0.3 ml and attached to the inner arm upper arm for 3 hours.

After 24 hours, the color of the brown-colored area was measured by DHA to compare the color difference with that before the sample application. Thereafter, while applying the sample of Example 1 twice daily, the degree of discoloration was measured by a chromameter every day, and the time taken to return to the original skin color was measured. Keratin peel rate was calculated by the following equation (1).

** T (Turnover Time): The time it takes for the old stratum corneum to be replaced by the new stratum corneum (in this case, the time it takes for the skin colored by DHA to fully revert).

Type of sample Turnover Time ( days ) Keratin peeling rate (%) Control group 18.6 - Alpha hydroxy acid (5% by weight) 15.2 18.14 Mycelium Extract (5% by weight) 12.7 31.66 Fruiting body extract (5% by weight) 13.5 29.14

*** Control: DHA No sample applied after staining with

As shown in Table 2, in the case of the manta mushroom extract, the turnover time, which is the time taken for the existing keratin layer to be replaced by the new keratin layer, is short, and the keratin peeling rate is 31.66% and 29.14%, respectively. Indicated. In particular, it was found that the mycelia extract is very excellent in the peeling effect.

In addition, the extract of the mantle mushroom showed a keratin exfoliation effect of about 18.14% in the case of 5 wt% alpha hydroxy acid (Lactic acid) which is a conventional keratin exfoliant, the extract used in the composition for promoting exfoliation of the present invention Silver showed 1.61 to 1.75 times better exfoliation effect than alpha hydroxy acid at the same concentration.

< Experimental Example 2> skin exfoliation effect

In order to measure the skin keratin improving effect of the mushroom extract of Example 1, 40 volunteers were surveyed after measuring the keratin improving effect.

Specifically, the composition containing 5% by weight of the manta mushroom fruiting body extract on the right foot for each 20 men and women 25 to 50 years of age. After the left sole, purified water was used for two weeks in the morning and evening. In case of remarkable effect on exfoliation effect, it was evaluated as 5 points with 5 points and no effect at all.

division Control (left) Experimental group (right) Exfoliation effect 5 One

As can be seen from the above experimental results, it was found that the cosmetic composition containing the extract of Manta mushroom as in the present invention is very excellent in exfoliation of the feet.

< Experimental Example 3> toxicity test

Cytotoxicity test of the mushroom extract of Example 1 was carried out. After 24 hours of inoculation of the cells into 96-well plates, the mushroom extract was treated at a constant concentration and cultured in an incubator at 37 ° C., 5% CO ₂ . After 48 hours of incubation, the medium was removed and 100 μl of MTT (3- (4,5-dimethythiazol-2-yl) -2,5-diphenyltetrazoliu, bromide) solution was added and reacted at 37 ° C. for 4 hours. After the reaction, the solution was removed, and 100 μl of DMSO was added to dissolve the stained cells, and then the absorbance was measured at a wavelength of 540 nm using an ELISA reader. Cell viability was calculated using Equation 2 below. As a result, the cells treated with the extract of 0.5 g / ml or 1 mg / ml of Example 1 showed cell viability of 98.2% to 98.9% when compared to the untreated group (Table 4).

Cytotoxicity of Extracts content Untreated group Mycelium Extract Fruiting body extract Cell survival rate (%) 0.5 mg / ml 100 98.2 98.9 1 mg / ml 100 96.4 97.1

The composition of the present invention can be prepared in the following formulations, the following formulations are merely illustrative of the present invention, whereby the content of the present invention is not limited.

< Formulation example 1> flexible lotion

Formulation example of the flexible lotion containing the mushroom extract was prepared as shown in Table 5.

ingredient Content (% by weight) Mesh Mushroom Extract 4.0 Polyoxyethylene Cured Castor Oil 0.4 Glycine 3.5 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example 2> nutrition lotion

Formulation example of a nourishing lotion containing the extract of Manchurian mushroom was prepared as shown in Table 6.

ingredient Content (% by weight) Mesh Mushroom Extract 4.0 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoine 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.2 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example 3> nutrition essence

Formulation example of a nutritious essence containing the extract of Manchurian mushroom was prepared as shown in Table 7.

ingredient Content (% by weight) Mesh Mushroom Extract 7.0 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyuronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl Cellulose 0.2 ethanol 3.0 Polyoxyethylene Cured Castor Oil 1.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example 4> nutrition cream

Formulation example of a nutritious cream containing a mushroom extract was prepared as shown in Table 8.

ingredient Content (% by weight) Mesh Mushroom Extract 10.0 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated Lecithin 1.0 Octyldodecanol 3.0 Trioctanoine 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example 5> Foot cream

Formulation example of the cream containing the extract of mango mushroom was prepared as shown in Table 9 below.

ingredient Content (% by weight) Mesh Mushroom Extract 12.0 Disodium ID (E.D.T.A-2na) 0.03 Glycerine 6.0 Urea 2.0 Acerola EXT 0.05 Rice Extract (Rice EXT) 0.05 Green Tea EXT 1.5 Propolis Extract (Porpolis EXT) 1.5 Phenoxy Ethanol 0.24 Carbopol # 940 (1%) 0.08 Cetearyl Alcohol (kalcol 6870) 2.0 Stearic acid 3.0 Micro wax 2.0 Glycerin Stearate KM105 0.8 Glycerin Stearate KM205 0.8 Methyl Paraben (D-M) 0.16 Propyl Paraben (D-P) 0.08 Srubitan sesquioleate (Arlacel # 83) 0.08 Polysorbate (Tween # 60) 0.8 Liquid Paraffin (L.P # 70) 8 Grape Seed Oil 2 Menthol 1.5 Dimethicone (D / C 200/100) 3.0 Tocopherol (Vit-E) 0.02 Beeswax 0.08 Keratinase 0.2 Triethanol Amine (T.E.A) 0.24 Purified water Balance

Claims

A cosmetic composition for promoting keratin exfoliation, which comprises a extract of manta mushroom as an active ingredient.

The cosmetic composition for promoting keratin exfoliation according to claim 1, wherein the extract of Manta mushroom is extracted with a solvent selected from the group consisting of water, C ₁ to C ₃ alcohols, and mixtures thereof.

The cosmetic composition for promoting exfoliation of keratin exfoliation according to claim 1, wherein the extract of the mandarin mushroom is a fruit extract of the mango mushroom.

The cosmetic composition for promoting keratin exfoliation according to claim 1, wherein the cosmetic composition is formulated in a formulation selected from the group consisting of cream, softening cream, converging cream, milk lotion, foundation, soap, and cleansing foam.

The cosmetic composition according to claim 4, wherein the cream is a foot cream for exfoliating feet.

The method of claim 5, wherein the extract of the manure mushroom is contained in the cream composition of the cream of 0.1 to 20% by weight.

The cosmetic composition for promoting keratin exfoliation according to claim 1, further comprising a keratin degrading enzyme.