KR20120078245A - A preparation method for pse extract of prunella vulgaris having antioxidative effect and the cosmetic composition comprising the same - Google Patents

Abstract

PURPOSE: A composition containing Prunella vulgaris PSE extract is provided to ensure antioxidative and antibacterial effects. CONSTITUTION: A method for preparing Prunella vulgaris PSE(pressed solvent extract) extract with antioxidative and antibacterial effects comprises: a step of pulverizing Prunella vulgaris; a step of injecting Prunella vulgaris using low carbon number alcohol, acetone, chloroform, methylene chloride, hexane, 1,3-butylene glycol, polyethylene glycol, glycerol, propylene glycol, or mixture solvent thereof; a step of extracting at 10-100°C using a PSE extractor for 30 minutes to 5 hours; and a step of filtering the extract solution at 10-100°C. A cosmetic composition with antioxidative and antibacterial effects contains the Prunella vulgaris PSE extract as an active ingredient. The composition is manufactured in a form of a lotion, skin, nutrition lotion, nutrition cream, essence, pack, cleansing agent, soap, and treatment.

Description

A preparation method for PSE extract of Prunella vulgaris having antioxidative effect and the cosmetic composition comprising the same}

The present invention relates to a method for producing a Prune PSE extract having an antioxidant effect and a cosmetic composition containing the extract as an active ingredient.

[Reference 1] Kim SY, Ryu KS, Lee WC, Ku HO, Lee HS, Lee KR., 1999, Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice, Korean J Pharmacogn , 30, 123-129.

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Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D., 1993, Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphan elderly study. Lancet, 342, 1007-1011.

[4] Kim JS, Kang SS, Lee KS, Chang SY, Won DH., 2000, Quantitative determination of ursolic acid from Prunellae herba. Kor J Pharmacogn, 31, 416-420.

[5] Sandra J., 1963, Phytochemical studies of Prunella vulgaris and Prunella grandiflora-Ⅰ. Saponin and triterpene compounds, Dissertations Pharm, 15, 333-341.

Document 6 Wang ZJ, Zhao YY, Tu GZ, Hong SL, Chen YY., 1999, Studies on the chemical constituents from Prunella vulgaris, Acta Pharm Sin., 34, 679-681.

Lam TL, Lam ML, Au TK, Ip DTM, Ng TB, Fong WP, Wan DCC., 2000, A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs , Life Sci., 67, 2889-2896.

[Reference 8] Ryu SY, Oak MH, Yoon SK, Cho DI, Yoo GS, Kim TS, Kim KM., 2000, Anti-allergic and anti-inflammatory triterpenes from the herb of Prunella vulgaris, Planta Med., 66, 358 -360.

Liu F, Ng TB., 2000, Antioxidative and free radical scavenging activities of selected medicinal herbs, Life Sci., 66, 725-735.

Document 10 Hodges, G.R., M.J. Young, T. Paul, and K.U. Ingold, 2000, How should xanthine oxidase-generated superoxide yields be measured, Free Radical Biol. Med., 29, 434-441.

[11] Kye-Ho Shin, Il-Young Kwack, Sung-Won Lee, Kyung-Hee Suh, Sung-Joon Moon, Ih-Seop Chang, 2007, Effects of Poyols on Antimicrobial and Preservative Efficacy in Cosmetics, J. Soc . Cosmet., Scientists Korea, 33 (2), 111-115.

As the life expectancy of modern people is prolonged, the incidence of senile disease is increasing. Mostly, free radicals, free radicals, and free radicals generated during normal cell metabolism in vivo due to excessive intake of nutrients, smoking, stress and excessive drinking, etc. It is known to cause disease by causing lipid oxidation, protein degradation and the like. ¹⁾ The oxidation process of lipids ^is also developed by free radical reaction, which degrades the quality of cosmetics and foods and causes the loss of nutrients. Antioxidants have been used to suppress such reactions, and antioxidants have been widely used as synthetic antioxidants, butylated hydroxytoluene (BHT) and tertiary butylhydroquinone (TBHQ), to improve economics and product stability. However, as the possibility of carcinogenic synthetic antioxidants is raised, interest in natural antioxidants is increasing. ²⁾ Flavonoids, anthocyanins, carotenoids, vitamins and essential oils present in natural products exhibit antioxidant activity by removing free radicals. Furthermore, natural antioxidants contained in plants have been reported to be effective in preventing various cancers and aging-related diseases caused by free radicals, and studies on antioxidant activity of natural products are being actively conducted. ³⁾

Prunella vulgaris is a perennial herb that belongs to Labiatae and has a corolla with upwards, and has four branches in the calyx, and in Chinese medicine, it is called dried seaweed in the ground, and is called blue liver, diuresis, anti-inflammatory, antipyretic and it is known that the efficacy of such blood pressure. ^4). The vinegared starch contains ursolic acid ^{4 )} , caffeic acid, rosmarinic acid, carotenoids, tannins, organic acids, etc. ^{5 )} , and saponins and triterpenes (from flowers). triterpene), flavonoids, sterols (sterol) and the fatty acid, etc. the reported ^6). The bioactive surface human immunodeficiency virus type hot-water extract from a sentinel (human immunodeficiency virus type) -1 inhibitory ^{activity. 7),} has been reported anti-allergic activity ⁸⁾ and antioxidant activity. ^9).

Accordingly, the present inventors completed the present invention by checking the physiological activity such as antioxidant activity and antimicrobial effect by extracting the vinegar using PSE (Pressed solvent extract) extraction method instead of general solvent extraction.

In order to achieve the above object, the present invention is a Prunella ( Prunella) vulgaris ) Provides a cosmetic composition having an antioxidant and antimicrobial effect containing PSE extract as an active ingredient.

Hereinafter, the preparation method for obtaining the PSE extract of the present invention will be described in detail.

The extract, as defined herein, may be dried after pulverizing dried vinegar using a mill, and then lower alcohols having 1 to 4 carbon atoms including purified water, acetone, chloroform, methylene chloride, hexane, 1,3-butyrene glycol, Polyethylene glycol, glycerol, propylene glycol, or a mixed solvent thereof, preferably 1,3-butylene glycol, glycerol, propylene glycol, more preferably 1,3-butylene glycol solution to the weight ratio of the vinegar powder ( a first step of injecting so that w / w) is 1:20, preferably 1:15, and more preferably 1: 9; A second step of extracting the mixture for 30 minutes to 5 hours, preferably 2 to 4 hours using a PSE extractor at a temperature of 10 to 100 ° C., preferably 30 to 90 ° C .; The extracted solution provides a manufacturing method of obtaining a hachocho PSE extract having an anti-oxidant and antimicrobial effect, which comprises a third step of producing a filter at a temperature of 10 to 100 ℃, preferably 30 to 60 ℃.

The extract as defined herein is a lower alcohol having 1 to 4 carbon atoms including purified water, acetone, chloroform, methylene chloride, hexane, 1,3-butylene glycol, polyethylene glycol, glycerol, propylene glycol or a mixed solvent thereof, preferably Preferably 1,3-butylene glycol, glycerol, propylene glycol, more preferably extracts soluble in 1,3-butylene glycol solution.

The present invention is the Prunella ( Prunella) vulgaris ) extract is characterized by being extracted using a PSE (Pressed solvent extract) extraction method.

The present invention provides a cosmetic composition having an antioxidant and antimicrobial effect containing the extract obtained by the above production method as an active ingredient.

The PSE extract obtained by the extraction method was able to confirm that it showed a stronger antioxidant and antibacterial effect than the Hachocho extract prepared by the conventional extraction method.

The cosmetic composition of the extract may be used in an amount of 0.001 to 90% by weight, preferably 0.01 to 10% by weight based on the total weight of the composition.

In addition, the cosmetic composition comprising an extract of the present invention includes a lotion, skin, lotion, nutrient lotion, nourishing cream, massage cream, essence, pack, cleansing, face wash, soap, treatment, essence.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-alpha tocopherol, D-alpha tocopherol, D-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, DL-alphatocopherol acetate, DL-alpha tocopherolvitamin E. DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenylethyl nicotinic acid) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be cosmetically blended. Preferably, seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like, and calginane, arginic acid, sodium arginate and argin purified from these seaweed extracts. Potassium ginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Other components that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, limiting agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopenta lysitol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearate isostearic acid , Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauric lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, citric acid Triether, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate Dioctyl sebacate, stearic acid cholesterol, isostearic acid cholesterol, hydroxystearate cholesterol, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid phyteryl, phosphate oleate , 12-stealoyl hydroxystearate isocetyl, 12-stealoyl hydroxystearate stearic acid, 12-s Allo one hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

As silicone fats and oils, polymethine silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methyl steoxysiloxane copolymer, and alkyl modified silicone Oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based oil include perfluoropolyether and the like.

As animal or vegetable fats and oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil , Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum, Marker demia nut oil, Meadow home oil, Egg yolk oil, Ujima oil, Mink oil, Orange rape oil, Jojoba oil, Canderry wax, Carnabas wax And animal or plant fats and oils, such as liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As a water-soluble low molecular moisturizer, serine, glutamine, sorbitol, mannitol, pyrrolidone- carboxylate titanium, glycerin, propylene glycol, 1, 3- butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned. Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters. As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose , Water soluble chitin, chitosan, dextrin and the like. Examples of the fat-soluble polymers include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular silicone.

     Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Examples of nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE glycerin fatty acid esters, and POE alkyls. Ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE POP (polyoxyethylene polyoxypropylene) copolymer, POE POP alkyl ether, polyether modified silicone, lauric acid alkanolamide, alkylamine oxide And hydrogenated soybean phospholipids.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

Examples of cationic surfactants include alkyltriethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylmenzyl ammonium, behenyltrimethylammonium, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and composites thereof; Polyamide, Polyester, Polypropylene, Polystyrene, Polyurethane, Vinyl Resin, Urea Resin, Phenol Resin, Fluoro Resin, Silicon Resin, Acrylic Resin, Melamine Resin, Epoxy Resin, Polycarbonate Resin, Divinylbenzene Styrene Copolymer, Silk Organic pigments such as powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentel salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cyanic acid octyl, daparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, uro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4-methoxydibenzoylmethane, 2,4,6-trianilino-ρ- (carbo-2-ethylhexyl-1-oxy) -1,3, 5-triazine, 2- (2-hydroxy-5 -Methylphenyl) benzotriazole, etc. are mentioned.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More preferably, Is formulated at 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors and Carrier.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , Fatty acid esters of 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carriers such as water, ethanol or propylene glycol, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hagocho PSE extract of the present invention has a high antibacterial effect and antibacterial effect through the evaluation of epidermal fungi, it can be usefully used as a cosmetic composition having an antioxidant and antimicrobial effect.

1 is a diagram showing the SOD activity (%) of Prunella PSE extract,
Figure 2 is a diagram showing xanthine oxidase inhibition (%) of Prunella PSE extract,
Figure 3 is a diagram showing the electron donating ability (%) of Hachocho PSE extract.

Below, The invention is illustrated in detail by the following examples and experimental examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Example  One. Hagocho PSE  Preparation of extract

The vinegar samples were dried and used at OmniHub ( http://www.omniherb.com/ ). 1500 g of the dried sample was crushed into a powder of coarse particles using a grinder (Attron Mix DA337, Daesung Atron) to prepare a diluted 1,3-butylene glycol solution (No.107-88-0, Celanese). , USA) and the weight ratio (w / w) was injected 1: 9 and extracted for 3 hours using a PSE extractor (Auto Vacuum Paddle Mixer, Han Yang, Korea) at 85 ℃. The extracted solution was used as a material of Experimental Example to obtain 1800g of haeju PSE extract (hereinafter referred to as "PV-PSE") of the liquid type after 100μm filtration at 40 ~ 50 ℃.

Experimental Example  One. Electron donating activity  ( DPPH ) Measurement

In order to confirm the effect on the electron donating ability of the samples obtained in the above example, experiments were performed using the method of Blois (Blois MS.Antioxidant determination by the use of a stable free radical. Nature 1958; 26: 1199-1120.

40 μl of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution was added to 160 μl of each sample solution, stirred for 30 minutes, and the absorbance was measured at 517 nm. At this time, the diluting solvent of the sample was used as MeOH, DPPH radical scavenging ability was expressed as the absorbance reduction rate of the addition and no addition group of the natural product sample solution.

DPPH is purple in a stable state, but when it is reduced and the radical is removed, its color loss is lost. By using this, the free radical scavenging ability of the natural extract can be effectively measured by measuring the decrease in absorbance at 517 nm.

As a result of experiments at the concentration of 0.1 μg, 0.5 μg, 1 μg, 5 μg, 10 μg / ml of the Hagocho extract, it was shown in FIG. 1 and showed strong concentration of electron donating activity (see FIG. 1).

Experimental Example  2. SOD  Active effect measurement

In order to confirm the effect on the SOD activity of the samples obtained in the above Example was carried out using the nitroblue tetrazolium (NBT) reduction method as follows (Beauchamp C, Fridovich I: Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-287, 1971 ).

0.1 ml of each sample solution and 0.6 ml of 0.1 M potassium phosphate buffer (pH 7.5) were added, and 1 ml of xanthine (0.4 mM) and NBT (0.24 mM) was added to the substrate solution, and 1 ml of xanthine oxidase (0.049 U / ml) was added. After the reaction for 20 minutes at 1N HCl 1ml to terminate the reaction, the amount of superoxide anion radicals produced in the reaction solution was measured at 440nm using a microplate reader (Sunrise Remote, Tecan, Austria).

Superoxide dismutase (SOD), one of the antioxidant enzyme is very harmful superoxide anion radical (O ₂ ^-) in vivo with an enzyme to generate a reaction with the _{hydrogen peroxide (H 2 O 2)} , present in all the species which consume oxygen It is a representative free radical inhibitor which has a protective action against free radical disorder in vivo.

As a result of measuring the deep superoxide dismutase associated with the anti-aging effect of the cell aging, as shown in FIG. It was shown to have (see Figure 2).

Experimental Example  3. Xanthine oxidase  Activity Inhibition Effect Measurement

In order to confirm the inhibitory effect of the Xanthine oxidase activity of the samples obtained in the above example, the experiment was performed by applying the method of Stirpe and Cort (Stirpe, F. and Corte, ED: The regulation of rat liver xanthine oxidase. J. Biol. Chem., 244, 3855-3861, 1969).

Substrates and enzymes were prepared by dissolving the substrate 2mM xanthine and the enzyme 0.2U / ml xanthine oxidase in 0.1M potassium phosphate buffer (pH 7.5). After 0.2 ml of extract was added to 0.2 ml of 2 mM xanthine (substrate), 0.2 ml of 0.2 U / ml xanthine oxidase was added and reacted at 37 ° C. for 20 minutes and 1 ml of 1N HCl was added to stop the reaction. After the reaction solution was centrifuged, the absorbance was measured at 292 nm for the content of uric acid. At this time, the control group used 0.2 ml of 0.1 M potassium phosphate buffer (pH 7.5) instead of the extract, and the blank used 0.1 M potassium phosphate buffer (pH 7.5) instead of xanthine oxidase. Blanks were measured using 0.2 ml of 0.1 M potassium phosphate buffer (pH 7.5) instead of xanthine oxidase and extracts.

xanthine oxidase is in vivo purine (purine) the substrate with the enzyme involved in the metabolism xanthine or react with hypoxanthine generate uric acid (uric acid) and superoxide in an intermediate product of the reaction (O ₂ ^-) and hydrogen peroxide (H ₂ O ₂ Is an enzyme that produces ¹⁰⁾

As a result of measuring the xanthine oxidase, it appeared as shown in Figure 3, and when the various concentrations of 0.1 ㎍, 1 ㎍, 10 ㎍, 100 ㎍ / ml was applied, the higher the concentration of the vinegar extract showed a stronger effect (see Figure 3).

Experimental Example  4. Dermal fungus  Antimicrobial efficacy evaluation

In order to confirm the antibacterial effect of the dermal fungus of the samples obtained in the above Example was performed as follows using a paper disc method.

That is, each strain cultured on a plate medium (Staphylococcus aureus (Staphylococcus aureus, KCTC 1621) and Staphylococcus epi more misses (Staphylococcus epidermidi , KCTC 1917)), take about 1 platinum, and incubate for 18 to 24 hours in 10 ml of liquid medium, inoculate 0.1 ml of the bacterial solution in 10 ml of liquid medium, and incubate for 3 to 6 hours. Inoculated approximately 10 ⁷ cells and evenly spread with a sterile swab. Place sterilized filter paper discs (Tokyo, 8 mm, Japan) on a solid plate medium, absorb the samples by concentration to make 0.05ml / disc each, and incubate at 35 ℃ for 18 ~ 24 hours to clear zone (mm) ) The diameter of the bacterial growth inhibition region was measured.

Generally, Staphylococcus aureus and Staphylococcus epidermis, which are bacteria that can cause skin irritation among dermatophytes, epidermidis) was used to confirm the efficacy of the clear zone.

The experimental results, Staphylococcus epi more misses (Staphylococcus epidermidis ) showed no significant antimicrobial activity, but showed high antimicrobial activity in a concentration dependent manner in Staphylococcus aureus as shown in Table 1. 1,3-butylene glycol (BG) used as a solvent of the extract is widely used as a moisturizing ingredient and a solvent, and it is known to increase antibacterial and antiseptic properties at high concentrations. According to the results of measuring the antimicrobial activity of chemical preservatives and regression analysis on Staphylococcus aureus, 1,3-butylene glycol is reported to increase the antiseptic and antimicrobial activity by 1.8 ~ 8.4%. It can be explained that it is associated with showing a slight antimicrobial effect even in the control containing no extract (see Table 1). ¹¹⁾

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation example  1. Cream composition

The oil phase and the water phase are heated and mixed at 75 ° C., respectively, and then cooled to room temperature.

Formulation example  2. Massage cream  Composition

The oil phase and the water phase are each dissolved by heating at 75 ° C., and then cooled to room temperature.

Formulation example  3. Lotion Composition

The oil phase and the water phase are heated, mixed and emulsified at 75 ° C., respectively, and cooled to room temperature.

Formulation example  4. Skin Lotion Composition

The aqueous phase and the ethanol phase are each prepared, mixed and filtered.

Formulation example  5. Essence Composition

The aqueous phase and the ethanol phase are each prepared, mixed and filtered.

Formulation example  6. Pack Composition

The aqueous and ethanol phases are dispersed, dissolved and mixed, and then cooled to room temperature.

Formulation example  7. Cleansing Foam  Composition

The aqueous and oil phases are dispersed, dissolved and mixed, and then cooled and cooled to room temperature.

Claims (6)

After drying the dried vinegar using a grinder, a lower alcohol having 1 to 4 carbon atoms including purified water, acetone, chloroform, methylene chloride, hexane, 1,3-butylene glycol, polyethylene glycol, glycerol, and propylene glycol Or a first step of injecting a mixed solvent thereof such that the weight ratio (w / w) with the edible powder is 1:20; A second step of extracting the mixture for 30 minutes to 5 hours using a PSE extractor at a temperature of 10 to 100 ° C .; The extracted solution is a method for obtaining a hachocho PSE extract having an anti-oxidant and antimicrobial effect comprising a third step of the manufacturing process filtered at a temperature of 10 to 100 ℃. The method of claim 1,
Prunella obtained through the manufacturing method ( Prunella vulgaris ) Cosmetic composition having an antioxidant and antimicrobial effect containing PSE extract as an active ingredient. The method of claim 1,
The extract is a cosmetic composition soluble in lower alcohol, acetone, chloroform, methylene chloride, hexane, 1,3-butylene glycol, polyethylene glycol, glycerol, propylene glycol or a mixed solvent thereof having 1 to 4 carbon atoms including purified water. The method of claim 1,
Prunella vulgaris ) extract is a cosmetic composition characterized in that the extraction using PSE (Pressed solvent extract) extraction method. The method of claim 1,
The extract is a cosmetic composition, characterized in that contained in 0.001 to 90% by weight (%) relative to the total weight of the composition. The method of claim 1,
The composition is a cosmetic composition, characterized in that the formulation selected from the group consisting of lotion, skin, lotion, nutrition lotion, nourishing cream, massage cream, essence, pack, cleansing, face wash, soap, treatment, essence.

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