KR20120072871A - Single nucleotide polymorphism (snp) markers associated with unsaturated fatty acid in pig and their methods for evaluation - Google Patents

Abstract

PURPOSE: A method for providing information for determining porcine unsaturated fatty acid content is provided to simply determine marbling score, oleic acid or linoleic acid content of pork and to early select and improve pigs of high quality. CONSTITUTION: A method for providing information for determining porcine unsaturated fatty acid content comprises: a step of isolating DNA from a pig; and a step of amplifying DNA using a prime pair for amplifying an amino acid sequence selected from sequence numbers 1-27 and 29-42 having an SNP selected among A↔C, A↔G, T↔C and T↔G in 61th base. The unsaturated fatty acid is oleic acid or linoleic acid. A method for providing information for determining pork of high quality comprises a step of isolating DNA from a pig and a step of amplifying DNA using the prime pair.

Description

Single nucleotide polymorphism (SNP) markers associated with unsaturated fatty acid in pig and their methods for evaluation}

The present invention relates to an information providing method for confirming the unsaturated fatty acid content of pigs, or a method for providing information for confirming the meat quality of pigs.

Korean pigs make up a very small share in the pig industry, but brand pork using pork is being produced in Jeju and Gangwon, and Korean pork is known for its high quality. However, in the domestic pig industry competition system, livestock farmers are avoiding production because their profitability is very low due to low breeding capacity and growth rate compared to the feed cost of conventional pigs.

Therefore, in order to develop Korean native pigs into competitive varieties with world-class high-quality pork such as Iberian pig, Spain, it is possible to use them for differentiated high-quality pork production by identifying the genetic characteristics of conventional pigs as well as systematic specification management. To date, there is a lack of research to identify the genetic characteristics of conventional pigs.

On the other hand, the conventional method of selecting pigs for producing high-quality pork is using a method based on phenotype related to fat traits. This is because fat affects the taste and texture of pork. However, ingesting a large amount of saturated fatty acids, which make up the fat of meat, is harmful to the health of consumers and causes negative perception of meat eating. Recently, research has been conducted to find genetic factors that regulate fatty acid composition (saturated fatty acids / unsaturated fatty acids) to produce high-quality beef. However, in Korea, where pork consumption is higher than beef, studies on the genetic factors of pigs are still insufficient.

Accordingly, the present inventors have recognized that fatty acid composition and content in pork have an important effect on fat metabolism and taste quality of pork, and have discovered a genetic site for controlling unsaturated fatty acid content in meat possessed by conventional pork, Crude fat content (IMF), intramuscular fat (MAR), meat cholesterol (CHOL) and fatty acid traits oleic acid (C18: 1) and linoleic acid (C18: 2) Quantitative trait loci (QTL) were identified and analyzed for candidate genes and genes that regulate them to develop biomarkers associated with their phenotypes, helping markers for the production of high-quality pigs. The molecular markers required for the selection (MAS) technique have been developed.

It is an object of the present invention to provide a method of providing information for determining the unsaturated fatty acid content of pigs.

Still another object of the present invention is to provide a method of providing information for identifying pork meat.

Still another object of the present invention is to provide a kit for identifying unsaturated fatty acid content or high quality of pork.

Still another object of the present invention is to provide a monobasic polymorphism (SNP) marker for identifying unsaturated fatty acid content or high meat content in swine.

One embodiment of the invention, the step of separating the DNA from the pig; And

Any one selected from the group consisting of SEQ ID NOs: 1 to 27 and 29 to 42 having any single base mutation selected from the group consisting of A↔C, A↔G, T↔C, and T↔G in SEQ ID NO: 61 It provides a method of providing information to determine the unsaturated fatty acid content of the pig, characterized in that for amplifying the separated DNA using a primer pair capable of amplifying the amino acid sequence of. The unsaturated fatty acid may be oleic acid or linoleic acid, but is not limited thereto.

Another embodiment of the invention, the step of separating the DNA from the pig; And

Any one selected from the group consisting of SEQ ID NOs: 1 to 27 and 29 to 42 having any single base mutation selected from the group consisting of A↔C, A↔G, T↔C, and T↔G in SEQ ID NO: 61 It provides a method of providing information identifying the pork meat, characterized in that it comprises the step of amplifying the separated DNA using a primer pair capable of amplifying the amino acid sequence of.

Another embodiment of the present invention, SEQ ID NO: 1 to 27 and 29 having any one base mutation selected from the group consisting of A↔C, A↔G, T↔C and T↔G in the base sequence 61st Provided is a kit for identifying unsaturated fatty acid content or high meat content in pigs comprising a primer pair capable of amplifying any one amino acid sequence selected from the group consisting of 42 to 42.

Another embodiment of the present invention, SEQ ID NO: 1 to 27 and 29 having any one base mutation selected from the group consisting of A↔C, A↔G, T↔C and T↔G in the base sequence 61st Provided is a monobasic polymorphism (SNP) marker for identifying unsaturated fatty acid content or high meat content in pigs, characterized in that it comprises any one amino acid sequence selected from the group consisting of from 42 to 42. The unsaturated fatty acid may be oleic acid or linoleic acid, but is not limited thereto.

Another embodiment of the invention, the step of separating the DNA from the pig; And

Amplifying the isolated DNA using a primer pair capable of amplifying the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30, the unsaturation of pigs Provide an informational method for determining fatty acid content. The unsaturated fatty acid may be oleic acid or linoleic acid, but is not limited thereto.

Another embodiment of the invention, the step of separating the DNA from the pig; And

Amplifying the separated DNA using a primer pair capable of amplifying the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30; Provides information on how to check meat quality.

Another embodiment of the present invention, for identifying the unsaturated fatty acid content or high quality of pigs comprising a primer pair capable of amplifying the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30th Provide the kit.

Another embodiment of the present invention, the monounsaturated polyunsaturated polyunsaturated fatty acid content of the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30 Provide (SNP) markers. The unsaturated fatty acid may be oleic acid or linoleic acid, but is not limited thereto.

As used herein, "high meat" is not limited thereto, but the content of oleic acid or linoleic acid, which is a type of unsaturated fatty acid, or high intramuscular fat, makes marbling of pork well, and is excellent in meat quality. It can be interpreted to mean that it is rich in taste and excellent in quality.

As used herein, the term “primer” refers to a single- which can serve as an initiation point for template-directed DNA synthesis under suitable conditions (ie, four different nucleoside triphosphates and polymerases) in a suitable buffer at a suitable temperature. Refers to stranded oligonucleotides. Suitable lengths of primers are typically 15-30 nucleotides, although varying with various factors, such as temperature and the use of the primer. Short primer molecules generally require lower temperatures to form hybrid complexes that are sufficiently stable with the template. The sequence of the primer does not need to have a sequence that is completely complementary to some sequences of the template, and it is sufficient to have sufficient complementarity within a range capable of hybridizing with the template to perform the primer-specific function. Therefore, the primer in the present invention does not need to have a sequence that is perfectly complementary to the nucleotide sequence of the template gene, and it is sufficient to have sufficient complementarity within a range capable of hybridizing to the gene sequence to act as a primer.

According to the present invention by the above-mentioned problem solving means, it is possible to determine the intramuscular fat degree, marbling, oleic acid, or linoleic acid of pork in a simple way, and based on this, it is possible to premature Korean pigs and improved pigs with excellent pork quality. It can be usefully used for selection and improvement.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

Example  1. Pig fat and Basic statistics  collection

Sixty Korean native pigs (KNP) and eight Yolkshire (YS) species were produced through full-sib at Samter Farm in Cheongwon-gun, Chungbuk, Korea to collect fatty traits. The crude fat content, intramuscular fat and cholesterol were measured using 349 F2 populations produced from 62 F1 offspring, and 237 unsaturated fatty acids were measured. The body fat of the F2 group was measured from the abdominal fat and subcutaneous fat, and the general component analysis such as cholesterol and intramuscular fat in the fillet. In addition, fatty acid traits were pulverized with 10 ml chloroform / methanol (2: 1) solution in a homogenizer (homogenize) of 200 mg of frozen fatty acid cross section (PT-MR3100, Polytron, switzerland). Then, after overnight, shaking was performed by adding 5 ml of 0.88% NaCl, followed by centrifugation, for further analysis of the chloroform fragment. After evaporation of the chloroform fragment, if nitrogen gas flows before 50 ° C., the fused sample is injected into a gas chromatograph machine and then a column injector and flame ionization detector with fused silica capillaries are maintained at 250 ° C. for the next procedure. Observations were made (every minute at 4 degrees gradually from the initial 50 degrees). Raised to 250 degrees. Ultra pure helium gas was used as a carrier and tridecanoic acid was used as an internal marker. As a method for determining the retention time, chromatogram correction was performed using a methyl ester mixture. Collected phenotypes and basal statistics are shown in Table 1 below.

characteristic Abbreviation Two observations Average Standard Deviation % Intramuscular Fat IMF 349 2.496 1.458 Intramuscular fat map (MAR) MAR 349 2.399 1.016 Cholesterol (CHOL) CHOL 349 142.026 84.385 % Myristic acid C14: 0 237 1.881 1.320 % Palmitic acid C16: 0 237 21.115 2.609 % Palmitoleic acid C16: 1 (n-9) 237 3.914 1.661 Stearic acid C18: 0 237 11.103 3.383 % Oleic acid C18: 1 (n-9) 237 31.970 5.402 % Linoleic acid C18: 2 (n-6) 237 12.899 6.396

The correlation between the collected fatty traits (IMF, CHOL, MAR) and unsaturated fatty acids (C18: 1, C18: 2) in fatty acid components is analyzed and shown in Table 2 below.

IMF CHOL MAR C18: 1 C18: 2 IMF One -0.384
(p <0.001) 0.622
(p <0.001) 0.288
(p <0.001) -0.230
(p <0.001) CHOL -0.384
(p <0.001) One -0.436
(p <0.001) -0.372
(p <0.001) -0.192
(p <0.005) MAR 0.622
(p <0.001) -0.436
(p <0.001) One 0.518
(p <0.001) -0.171
(p <0.01) C18: 1 0.288
(p <0.001) -0.372
(p <0.001) 0.518
(p <0.001) One -0.043
(p = 0.516) C18: 2 -0.230
(p <0.001) -0.192
(p <0.005) -0.171
(p <0.01) -0.043
(p = 0.516) One

As shown in Table 2, it was confirmed that when the content of intramuscular fat (MAR) increases, unsaturated fatty acid oleic acid (C18: 1) also increases (0.518). Intramuscular fat (MAR) and oleic acid (C18: 1) were positively correlated, and these two traits were the most important factors in producing high quality pork.

Example  2. Monobasic polymorphism ( SNP ) Analysis

DNA was isolated from the blood of the experimental population used in Example 1 to analyze SNP markers useful for determining meat quality (quality), such as muscle fat, cholesterol, marbling, oleic acid, or linoleic acid.

The genotypes of 1098 SNP markers distributed on the chromosome were analyzed using Illumina Porcine60K BeadChip to perform quantitative trait loci (QTL) related to fat and fatty acid traits on porcine chromosome 12. Among the 1098 SNP markers distributed on chromosome 12, markers with a large difference in genotype frequency between Korean native pigs and Yorkshire species (> 0.650) and high PIC (Polymorphic information contents) values of the markers The markers (> 0.325) of the final selection was made a chain map using a total of 33 SNP markers (see Table 3 below).

SSC Marker PIC ^a KNP vs. YS ^b cM bp ^c 12 ASGA0052791 0.372 0.738 0.0 2837957 12 ASGA0052835 0.369 0.788 0.3 2998823 12 H3GA0033256 0.372 0.771 0.6 3091941 12 M1GA0016062 0.373 0.771 0.9 3113162 12 H3GA0033394 0.350 0.713 6.1 4063048 12 MARC0025796 0.372 0.875 7.4 4329529 12 ASGA0053189 0.371 0.663 9.6 5914248 12 ASGA0053416 0.372 0.655 15.3 7772473 12 MARC0114829 0.364 0.679 15.7 7833935 12 MARC0020580 0.333 0.675 16.8 8307707 12 MARC0066314 0.354 0.762 21.5 9696755 12 H3GA0033784 0.373 0.652 22.4 9984578 12 ALGA0065527 0.361 0.650 24.2 10403555 12 DIAS0000091 0.350 0.775 26.7 10489480 12 MARC0071015 0.352 0.650 45.1 15877244 12 ASGA0054041 0.334 0.690 45.5 16112641 12 ALGA0065836 0.371 0.888 47.0 13908167 12 MARC0046077 0.374 0.750 49.9 17408164 12 DRGA0011754 0.370 0.833 52.4 19788635 12 ALGA0066299 0.374 0.713 53.5 20080072 12 ASGA0054460 0.371 0.700 55.9 20441504 12 ASGA0054485 0.371 0.881 56.7 20658103 12 ASGA0054522 0.364 0.750 59.4 20938787 12 ALGA0066582 0.359 0.825 65.6 22090783 12 ASGA0054746 0.363 0.775 66.3 22240687 12 ALGA0066610 0.374 0.667 66.8 22397509 12 ASGA0054801 0.369 0.675 67.9 23013143 12 DIAS0003408 0.326 0.702 69.2 23353861 12 H3GA0034642 0.374 0.726 74.5 25301666 12 ASGA0055015 0.354 0.738 75.1 25707462 12 ALGA0067055 0.371 0.788 80.6 27305775 12 ASGA0055300 0.375 0.717 89.7 28222408 12 MARC0016323 0.375 0.670 94.6 67065942

^a Polymorphic information content

^b The one allele frequency difference between Korean Native pig and Yorkshire

^c SNP marker Genomic map of IlluminaPorcine SNP60K BeadChip

A highly significant QTL region associated with oleic acid (C18: 1) was found at 52 cM (17408164-20080072 bp) between MARC0046077 marker and ALGA0066299 marker in chromosome 12 region (P> 0.01). Also, between 71 and 77 cM, that is, between DIAS0003408 and ALGA0067055 markers (23353861 to 27305775 bp), a highly significant QTL region related to crude fat (IMF), intramuscular fat (MAR) and linoleic acid (C18: 2) was found (P > 0.01) (see Table 4).

SSC Trait Locus (cM) F-value LOD Throshold Additive Dominance Imprinting 5% One% Estimate S.E. Estimate S.E. Estimate S.E. 12 IMF 71cM 9.790 ** 5.903 3.471 7.388 0.609 0.169 0.420 0.252 -0.723 0.181 12 CHOL 63 cM 8.170 ** 4.987 4.542 7.626 -31.459 7.994 -6.899 11.952 26.892 8.519 12 MAR 77 cM 14.270 ** 8.322 4.237 6.025 0.597 0.100 0.321 0.155 -0.292 0.115 12 C18: 1 52 cM 7.890 ** 4.830 4.347 6.277 1.641 0.408 1.580 0.581 -0.423 0.413 12 C18: 2 75 cM 5.330 ** 3.329 4.323 5.020 -1.130 0.299 -0.194 0.453 0.466 0.336

* p <0.05 for F-statistic threshold.

** p <0.01 for F-statistic threshold.

The 45 SNP markers present in the QTL regions related to crude fat (IMF), intramuscular fat (MAR), and linoleic acid (C18: 2), 23353861-27305775 bp, were significant QTL regions for oleic acid (C18: 1) content. 20 SNP markers within 17408164 ~ 20080072bp were observed. A total of 30 candidate genes were selected using www.ensembl.org/BLAST/BLAT for a total of 65 SNP markers present in the QTL region associated with the found fat and fatty acid components. The base sequences of candidate genes are shown in Table 5 below.

SEQ ID NO: SNP name Gene SSC Position SNP Flanking sequences One MARC0046077 MS12 12 17408164 [A / C] CTCCTCAGTTTGCAGACTGACCCAGGCTGGGGGGCAGCAGAGGGTGGGGAAACCCTCCCC [A / C] CCACCACCACCACACACACTGTGCTAAATCCTGGGTCCATCTCTGCTAGGAAAATGGCTT 2 DBWU0000835 ENSSSCG00000017625 12 17627829 [A / G] ACAGCGACTTGGAGAAACAGATGAAGAGAAGTTATTTGTGGAAGCAGGGAAGGCTCTATT [A / G] GCCGAAGGTGTCACTTTAAGACGAGTAGGAGAAGCAAGGACTC 3 H3GA0034221 ENSSSCG00000017624 12 17654890 [T / C] AAAAGCTGCCAATTCTTTCTTTGAGGCAGGCAGCCTGTGTGTGTGTGGCGGGGAGGGCAC [T / C] GGCAGAGCAGGGCCCACTCTGCATACACACAGAGGCAGAGTCCAGATGTCCAGTGTGCAACC 4 H3GA0034269 TUBD1 12 18873255 [T / C] AGAAAAAGACAGAAAGGCAAAAAAAAAAAAAAGAAAGAAAGAAAATGTCCCCAGACGTTG [T / C] CAAATGTTCCACGGCCAGTTACCACCAATCCCATTGAGAACCACCACCCTAGATAATGTC 5 ASGA0054400 GGNBP2 12 19752172 [T / C] TCACATCCTCACATATCCCAGTCAGGTTCATTACCACTGAGCTCCAAAGAAACAAAGAAA [T / C] AAAATTTAATTCATGTGCATGGAGATCCCATAGAAATGAGACCTAAAAAATGACCAAAGC 6 DRGA0011754 GGNBP2 12 19788635 [T / G] ACAAATGCAAGTTGAGTTGCATTTTATTCTTAACTTAGTACTTTCTGAGACAGAATGCCA [T / G] TATACTTATCCTTTAAACATCCCAGTTAAAGACTTGATATGACTGAATTGAAACTAAAAG 7 MARC0108602 AATF 12 19978010 [A / G] GCAATAGAAGCATAAGAGATAAGAGGACAACTGTTGGTGATAATAAGGATGGAAAACAGG [A / G] TTTAGCAACTGACGGAATGTATCAAGTAAGGGCACCAGCACGCCAGTGATCTCTGAAAAC 8 DRGA0011757 AATF 12 19997492 [A / G] GACTTAACATAGTTTGGGGTTTTGCCAGGGCAGTACAGGTAGGTATGTAATCCCTTATCT [A / G] CACTTCTGAAATTTAAAGAGGTCTGAAAATTCATAGTTATCTTGTTCGTTTGTTTTGGTA 9 ASGA0054436 AATF 12 20010666 [A / G] ACATTTAGAGTGCTACCTAATGAATAGTGTACATTTAAATAGCATGAGCCATAATTTGCA [A / G] AAAGCATGAAAGGAAATAAAAGCTTCAAGTGTCAGAATGGGATTCTGGGGCAGAGCAGCA 10 ALGA0066299 ACACA 12 20080072 [T / C] GGCACAGGGATCTGGGTCAACTAGGACGAAGTGGGGAAAAGGGTCTCCGACGCGTTCTGA [T / C] GACAGCTTCAGACGGGCAGAAGTTTAGGTTCAGTCTAAGAAAATGCCTGGCAGGTCCCTC 11 DIAS0003408 ENSSSCG00000017783 12 23353861 [A / G] GGAGGAACGAGACCAGCGCACGGCCGCTGAGAACCGGGAGAAGGAGCAAAACAAGAGGCT [A / G] CAGCGGCAGCTCCGAGACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAG 12 ASGA0054859 TAOK1 12 23716127 [T / G] TTCTTAGTCGGATTTGTTTCCACTGCACCATGACGGGAACTCCTTGAAGCTTATTTTAAA [T / G] CACTATCAGAAATAAATTTATTTGTACTCACAGGTATTCCTGATAATGAGAATATTTGTT 13 H3GA0034572 ANKRD13B 12 23932376 [T / C] AAGCGTGAAGGAACTTCAGGGGATCCAAAAGTGGCCCAGAGGGTGGGATAGGGCTGGTCA [T / C] GCCTCCTGTGCTCGGGCAGAACAACTTCGAGGAGCCAGTGGCACTGCAGGAGATGGACAC 14 ALGA0066706 SMG6 12 24142968 [T / C] AAGGAGAGTTAGGTTCCCTGGGTACGAAAGCAGCTAAACATGTTATCTACACAGCAAATA [T / C] GGGGGCACTTGTCAATCCAGACACTTTATACACATACATATAACCCAAATGGAATCCGTT 15 H3GA0034587 SMG6 12 24394985 [A / G] CCAGGAACTCCATATGCCACGGGGTAGCCAAAAAAAAAGGCAGTTATTTACCCTCAAGTA [A / G] TAATGGCAGCTCAGACCCAGGGCCGCCTCCAGTGAGAAAGACATCTCACTTCAGGAAATG 16 CASI0011743 SMG6 12 24416306 [A / G] GGTTTTACTCTAAGAGTTTTTACTCCAAAATAAAGATGGTACTCTCTTAGTACTTGTGCT [A / G] CACACCCACGGATGCATTCATTAGAGAAAAGCAGGACAGTCACAAAAGGCAACCACATTC 17 H3GA0034597 ENSSSCG00000017834 12 24823400 [A / G] AACCTCTTCCTCCAAGCACCGAGGCACCAAGACGTTGCCCATGACTGCCACTTAAAGTGG [A / G] CAAAATTAGTTTAGGCCCATTAAGATTTAGATCTTTATATGTGAATATGCAAAGCAATTT 18 H3GA0034642 OR3A1 12 25301666 [A / G] GCTCATGCGGCTGCTGTAAGTGAGGGGTCGGCAGATGGCCAAGAATCGGTCATAGGCCAT [A / G] GCTGTCAACAGGAAGCAGTCTACACCAACCAGGAGATGGAAGAAGAAAAGCTGTGTGAGG 19 ASGA0054989 ENSSSCG00000017860 12 25327915 [A / C] TTGGAGTTGAAACTTCCCAGATGCTATATGGAGAAATGCAGTATAGTCTTACTATCGAGA [A / C] CATCTACAGGCTGGTAGTCAGCAACTCTGGAGCTGGAGATGTCCTCGAATTCAGTTTCAT 20 M1GA0016889 ITGAE 12 25469736 [T / C] CCTCCTCCGCCCGAGCCTTCTGACCGTTACCCCTTCTCTCCCCGGGCCCTGCYGCCAGGC [T / C] GGGAAGTTCGACATCATCCCCACAATGACCACCATCGGCTCTGGCATTGGCATCTTCGGG 21 ASGA0055015 SLC13A5 12 25707462 [A / C] AAGAAAGTGACGAGGTGATGTGTGCACCTTTGTATCTTCAGCACACAAAACAAGGCTTAG [A / C] ACAGAAGAGCTCTAGGGACTTTGGGCAGGCGTTGGGGGAAAAGAATGAGCGCCCCTCTCAAG 22 M1GA0016908 SLC13A5 12 25721207 [T / G] CCACACGACTGCAGAAGACATCTGTCACACACCAACCCTGCCCCGTGTCCGGGCCCCACG [T / G] GAAAGGGAAGACGCTCTCACTGGTGCTTGGCTGCATAGTTTAGTTTTGATTGCAGTTCTT 23 H3GA0034675 KIAA0753 12 25778247 [A / G] TTCTGCCCGAGTGCTCTACGTCCTCCAGCAGCAGGTGCGGGATTCAGGGAGGGCCCCCCC [A / G] CCTTGTTTTTGTCTTCTCAGGGAATTCCTGTTTTCTTTCTGTCTCAGGGATGCCAGCACC 24 MARC0094795 KIAA0753 12 25802316 [A / C] ATTAGACATTAATCGCATTAACTGACCAGGGGAAAAAAGCATCATTTGGTGGCTATGCTT [A / C] TGTAAAACACTAAGCCATAGCACGCCTGGTCTGCAGAGCTACACGTAGGCTTTCAGCTTC 25 H3GA0034677 ENSSSCG00000017892 12 25856879 [A / G] GCTCCCAGGAGCAGGCCCAGGTCCACGCTGGCTTGCCCTCTCAGCGCTGGGGGCCAGGCC [A / G] TGAGTGTCTGGCTTTCTGGGCAGGCTCTGAAGGGTTATCTCCCTGCTTCTGTTCTCCAGT 26 ASGA0055028 ENSSSCG00000017892 12 25915182 [T / C] TGGACTGAGCCAGGACCTTGTCAGGACTCGACTGAGCTCTGACCTCAGCTAGGGCCCTTA [T / C] CCAACATTCAGTTCTCCCGGGAGACCCTGCAGACCAGTAGTCCTGGGGGGAGACGCTGCA 27 ASGA0055049 ASGR2 12 26106407 [A / G] TTTGTTTAATTTAATGCCAATTATACACATTTTGAAGAAGCCCTAGAAAAATTTCCAGCC [A / G] AGGACTGGGAAGCCTTGACCCAGCACAAGCTTAGAAGCAAAAATTGCTTGAAAGAGTAAT 28 MARC0014525 SLC16A13 12 26235647 [A / G] CCAGTGGAAGGAGGTKGACATGCAGGAAA [A / G] AGTTATCTGGGAAATGAGAATGGGGCAGCTAGCACCCGGGAGCACACCACCCCCTCTCAC 29 ALGA0066946 NEURL4 12 26381120 [T / C] CAGCGGCACCGACGACCCCGCCTTCGTCGAGGCAGCCAACACCTTGAAGTATTCGTTTTA [T / C] GGCCAGAGGAGGTGTCTTTCCTTCACGCCGTAGCTACGAAGTTGTGTGACCCGGGGCTGA 30 ASGA0055076 ATP1B2 12 26549245 [T / C] CCTTTGTTGGCTAGGCCTGATGATTCGTCCTAAGACTGAGAACCTTGATGTCATCGTCAA [T / C] GTCAGTGACACTGAAAGCTGGGACCAGCATGTTCAGAAGCTCAACAAGTTCTTAGAGCGT 31 ALGA0066960 WRAP53 12 26575981 [T / G] GGCTCACCTCTGCCACAGGTCTGCTCAGCTCAAGCCCAACACTCGCAAGATACAGGTCTA [T / G] CCAGGCTTCTCTCTCCTACTCCTAGGGCCCCTGATGGTTCCTGCATCCTGACCAATAGTG 32 M1GA0016958 TP53 12 26581252 [A / G] GTGAAAGCAGGGTGCCCGTTCCTGGGGTGGTCCTGAACCCCCGGGCCAATGGGCAGCTCT [A / G] TGAGATGGGAAGCTGCGGGCATGCGAGTTCTCTGTGCAACCTGCAAGGCACTCCTAGCCC 33 ASGA0055081 DNAH2 12 26595674 [A / G] AGTGGCTGGGGACACGTCTCTGCTTCCCAAAACCATATGGCAGCCGGTCTCCCAACGTTC [A / G] AAAGCTGGCAGAGTGCGTGTAGTTGGAGGCTCCAGCTCTGCTCAGGGCGGTGGTAAAAGG 34 ALGA0066969 GUCY2D 12 26671590 [A / G] CGGGCTGATCAGGGCAGTGTGGCAGCAGCCTGAAAGGCAGACCTGCAGCTGCTTTTATGT [A / G] GGGCTTGCCATGGGGGGCTTGGGACGCTCGGAGGGGAAAGAAGCTGCCTGGCTGGCTGCA 35 ALGA0066974 ALOX15B 12 26715540 [T / C] GGCTCAGGCCAACAGCCATCACAGAAGCCAGCTCCTCAGAAAGGAGCCACCTGGGGTTGA [T / C] AGATGCCAAAGTGTGAACATGGCTTTGACCCACAGCTGCAGGGGGGAGGAGCCCTGGGAG 36 ALGA0066986 NDEL1 12 26867891 [T / C] TCCCTTATACACAGTCTTGGTTAATTTTTATGCACTGTAAAACTGTTGCAAGGTTTTCTT [T / C] CTGAAAATGTGTAAGCTTGGTTATATTGTGTTTAAAATCCAGTGGGCAGATGTAAATTGT 37 H3GA0034753 NDEL1 12 26885737 [T / C] GTAAGAATTAAATGTTTTTGTGAGCTGGCCTTTTCTCAGGTGTTTAGGTTATATGGTAAC [T / C] GGATTGTATAGACCCACTTAAACACTAACCTGTTATTGCTGGAGTTGCATTCTCTGGTGG 38 ASGA0055114 MYH10 12 26934636 [A / C] TGCGTGGCGGGTTCTCAGTGCTCATTTACACGGGTGGCGACACTTATGCTCTAAGGTACA [A / C] TCTCAACAGCTAAAAATGATTCTGGAACAAACATTCAACTACCATGAATTCCCAGAAGCA 39 MARC0074335 MYH10 12 26957184 [A / G] CTTGTTAGATACTCTTCAGTGAGAGAAATACATTGTTTCTATGCTCTCATGTGCAGAAGA [A / G] ATCAGATTTTTCCTGTTCATTCAGAACTATATTTTA 40 ALGA0067003 MYH10 12 26997723 [T / C] TTTTTCTTACTTACTCTAAATACGAACCCTTGCGCCCCTAAAGTCTCCTGGCCTGAGCAA [T / C] GTCTTGAGAGCTCCACTTAGCACGTTTTTAGGGCTTTTCTGAACTAGATCACACATTGAA 41 DRGA0011818 NTN1 12 27199873 [A / G] TTGCGCCTCTGTAGTGACCTGAGCTGCTGCGGTCAGATTCTTAACCTGTTGCACCATAAC [A / G] GGAATTCCTTAGTTTGTTTTCTTTTAAAAAATGTATATGTGCTTACTTAGGAAGGTTTGG 42 ALGA0067055 NTN1 12 27305775 [A / G] GGGCTGCATTTAGTGGGAATGCTGATAAAGCACCGCCTTATGGCCAGAGGAAGCTAACCC [A / G] GGTAGCGATGGCATAGTACCAATGACCACAGAAGAGGAGAGATGTTGTCCCTCTAAGAAGGG

[A / B] in the above table means that base A has SNPs point-mutated to base B.

Candidate genes present in the QTL region (Nos. 1 to 10), 17408164 to 20080072 bp, which are significant for oleic acid (C18: 1). The most significant gene (No. 10) was ACACA (acetyl-coenzyme A carboxylase alpha) gene and the SNP sequence number was No. 10 (P <0.001) (see Table 6). ). Acetyl-Coenzyme A Carboxylase Alpha Enzyme is known to play a key role in the biosynthesis of long-chain fatty acids (FAs) in the conversion from acetyl-CoA to malonyl-CoA. Genes are being studied in pigs. In addition, candidate genes present in the QTL region 23353861 to 27305775 bp related to crude fat (IMF), intramuscular fat (MAR) and linoleic acid (C18: 2) were searched for a total of 23 candidate genes. As a result, the marker showing the most significant effect (P <0.0000000005) on intramuscular fat was ALGA0066986 (No. 36) in the NDEL1 gene, and this marker was very high in IMF (P <0.0001). . As can be seen from the results of this study, fat morphology is closely related to fatty acid composition, and genetically superior pigs are selected if the DNA markers presented in Table 6 are used in the selection index based on the existing phenotype. It is expected that the breeding and breeding will be possible, and the production of functional and high quality pork will be more accurate.

SNP name SSC Position
(bp)
AA AB BB Total beta_1 beta_1_sd beta_2 beta_2_sd Trait logp
P-value Function Gene MARC0046077 12 17408164 16 83 38 137 -1.459 0.534 1.575 0.663 C18: 1 2.528 <0.05 gene MS12 DBWU0000835 12 17627829 29 111 93 233 0.378 0.091 0.195 0.122 marbling 5.994 <0.0001 gene ENSSSCG00000017625 DBWU0000835 12 17627829 22 81 74 177 One 0.457 1.28 0.62 C18: 1 3.141 <0.005 gene ENSSSCG00000017625 H3GA0034221 12 17654890 38 127 68 233 -0.339 0.09 0.082 0.119 marbling 3.022 <0.005 gene ENSSSCG00000017624 H3GA0034269 12 18873255 48 76 91 215 -0.122 0.079 -0.401 0.128 marbling 3.058 <0.005 gene TUBD1 H3GA0034269 12 18873255 39 56 67 162 -0.596 0.383 -2.11 0.636 C18: 1 3.207 <0.01 gene TUBD1 ASGA0054400 12 19752172 77 110 47 234 -0.238 0.082 0.254 0.117 marbling 3.307 <0.01 gene GGNBP2 ASGA0054400 12 19752172 61 79 37 177 -1.109 0.391 1.398 0.575 C18: 1 3.471 <0.01 gene GGNBP2 ASGA0054400 12 19752172 61 80 38 179 0.718 0.286 -1.257 0.422 C18: 2 3.719 <0.01 gene GGNBP2 MARC0108602 12 19978010 15 84 78 177 0.761 0.529 1.615 0.669 C18: 1 3.382 <0.01 gene AATF DRGA0011757 12 19997492 78 83 15 176 -0.761 0.528 1.556 0.669 C18: 1 3.223 <0.01 gene AATF ASGA0054436 12 20010666 15 84 78 177 0.761 0.529 1.615 0.669 C18: 1 3.382 <0.01 gene AATF ALGA0066299 12 20080072 76 116 41 233 -0.21 0.086 0.184 0.12 marbling 2.2 <0.05 gene ACACA ALGA0066299 12 20080072 55 88 34 177 -1.359 0.403 1.5 0.564 C18: 1 4.516 <0.001 gene ACACA DIAS0003408 12 23353861 106 96 32 234 0.405 0.133 -0.035 0.19 IMF 2.39 <0.05 gene ENSSSCG00000017783 DIAS0003408 12 23353861 106 96 32 234 0.397 0.088 0.014 0.125 marbling 4.851 <0.001 gene ENSSSCG00000017783 DIAS0003408 12 23353861 79 73 27 179 -1.256 0.31 -0.344 0.449 C18: 2 3.501 <0.01 gene ENSSSCG00000017783 ASGA0054859 12 23716127 107 95 32 234 0.394 0.134 -0.002 0.191 IMF 2.16 <0.05 gene TAOK1 ASGA0054859 12 23716127 107 95 32 234 0.392 0.088 0.026 0.125 marbling 4.665 <0.001 gene TAOK1 ASGA0054859 12 23716127 79 73 27 179 -1.256 0.31 -0.344 0.449 C18: 2 3.501 <0.01 gene TAOK1 H3GA0034572 12 23932376 32 95 106 233 -0.405 0.133 -0.013 0.19 IMF 2.332 <0.05 gene ANKRD13B H3GA0034572 12 23932376 32 95 106 233 -0.397 0.088 0.021 0.125 marbling 4.798 <0.001 gene ANKRD13B H3GA0034572 12 23932376 27 73 79 179 1.256 0.31 -0.344 0.449 C18: 2 3.501 <0.01 gene ANKRD13B ALGA0066706 12 24142968 138 84 11 233 -0.626 0.135 -0.247 0.165 marbling 5.614 <0.0001 gene SMG6 H3GA0034587 12 24394985 110 106 18 234 -0.486 0.111 -0.284 0.14 marbling 4.033 <0.001 gene SMG6 CASI0011743 12 24416306 138 84 11 233 -0.626 0.135 -0.247 0.165 marbling 5.614 <0.0001 gene SMG6 H3GA0034597 12 24823400 40 92 44 176 -0.626 0.31 1.112 0.429 C18: 2 2.206 <0.05 gene ENSSSCG00000017834 H3GA0034642 12 25301666 32 104 88 224 -0.574 0.136 0.052 0.188 IMF 4.014 <0.001 gene OR3A1 H3GA0034642 12 25301666 32 104 88 224 -0.476 0.088 0.136 0.122 marbling 6.032 <0.00001 gene OR3A1 H3GA0034642 12 25301666 25 76 68 169 -1.278 0.45 -0.466 0.63 C18: 1 2.369 <0.05 gene OR3A1 H3GA0034642 12 25301666 25 78 68 171 1.164 0.324 0.224 0.451 C18: 2 3.355 <0.01 gene OR3A1 ASGA0054989 12 25327915 38 103 92 233 0.314 0.085 -0.087 0.122 marbling 2.877 <0.05 gene ENSSSCG00000017860 M1GA0016889 12 25469736 103 60 16 179 0.745 0.38 -0.577 0.527 C18: 2 2.139 <0.05 gene ITGAE ASGA0055015 12 25707462 50 110 73 233 -0.357 0.121 0.286 0.175 IMF 2.183 <0.05 gene SLC13A5 ASGA0055015 12 25707462 50 110 73 233 -0.374 0.08 -0.078 0.115 marbling 4.887 <0.001 gene SLC13A5 ASGA0055015 12 25707462 36 76 65 177 -0.932 0.393 -1.333 0.585 C18: 1 2.804 <0.05 gene SLC13A5 M1GA0016908 12 25721207 10 81 142 233 0.688 0.14 -0.253 0.169 marbling 6.673 <0.00001 gene SLC13A5 M1GA0016908 12 25721207 10 69 98 177 1.72 0.633 -0.39 0.782 C18: 1 2.184 <0.05 gene SLC13A5 H3GA0034675 12 25778247 49 111 74 234 -0.355 0.122 0.28 0.175 IMF 2.1 <0.05 gene KIAA0753 H3GA0034675 12 25778247 49 111 74 234 -0.387 0.08 -0.044 0.115 marbling 5.08 <0.0001 gene KIAA0753 H3GA0034675 12 25778247 35 76 66 177 -0.959 0.395 -1.325 0.586 C18: 1 2.897 <0.05 gene KIAA0753 MARC0094795 12 25802316 49 110 74 233 -0.355 0.122 0.3 0.175 IMF 2.178 <0.05 gene KIAA0753 MARC0094795 12 25802316 49 110 74 233 -0.387 0.08 -0.038 0.115 marbling 5.048 <0.0001 gene KIAA0753 MARC0094795 12 25802316 35 76 66 177 -0.959 0.395 -1.325 0.586 C18: 1 2.897 <0.05 gene KIAA0753 H3GA0034677 12 25856879 49 110 74 233 -0.355 0.122 0.3 0.175 IMF 2.178 <0.05 gene ENSSSCG00000017892 H3GA0034677 12 25856879 49 110 74 233 -0.387 0.08 -0.038 0.115 marbling 5.048 <0.0001 gene ENSSSCG00000017892 H3GA0034677 12 25856879 35 76 66 177 -0.959 0.395 -1.325 0.586 C18: 1 2.897 <0.05 gene ENSSSCG00000017892 ASGA0055028 12 25915182 28 88 118 234 -0.395 0.092 0.168 0.131 marbling 3.923 <0.01 gene ENSSSCG00000017892 ASGA0055028 12 25915182 22 68 89 179 1.131 0.335 -0.671 0.479 C18: 2 2.395 <0.05 gene ENSSSCG00000017892 ASGA0055049 12 26106407 68 109 56 233 0.39 0.119 -0.08 0.174 IMF 2.38 <0.05 gene ASGR2 ASGA0055049 12 26106407 68 109 56 233 0.427 0.077 -0.086 0.113 marbling 6.499 <0.00001 gene ASGR2 ASGA0055049 12 26106407 57 84 36 177 1.272 0.398 -1.261 0.57 C18: 1 3.678 <0.01 gene ASGR2 ASGA0055049 12 26106407 57 86 36 179 -1.093 0.298 -0.159 0.425 C18: 2 2.818 <0.05 gene ASGR2 MARC0014525 12 26235647 151 68 14 233 0.313 0.185 -0.237 0.245 IMF 2.053 <0.05 gene SLC16A13 MARC0014525 12 26235647 151 68 14 233 0.403 0.121 -0.152 0.16 marbling 5.438 <0.0001 gene SLC16A13 MARC0014525 12 26235647 112 54 11 177 1.085 0.6 -0.927 0.792 C18: 1 2.495 <0.05 gene SLC16A13 MARC0014525 12 26235647 114 54 11 179 -1.442 0.438 -0.073 0.579 C18: 2 3.503 <0.01 gene SLC16A13 ALGA0066946 12 26381120 14 108 104 226 -0.422 0.124 0.034 0.15 marbling 3.698 <0.01 gene NEURL4 ALGA0066946 12 26381120 11 78 81 170 -1.245 0.571 -0.421 0.699 C18: 1 2.341 <0.05 gene NEURL4 ALGA0066946 12 26381120 11 78 83 172 1.452 0.427 -0.516 0.523 C18: 2 2.86 <0.05 gene NEURL4 ASGA0055076 12 26549245 51 116 66 233 0.441 0.122 0.066 0.173 IMF 2.849 <0.05 gene ATP1B2 ASGA0055076 12 26549245 51 116 66 233 0.421 0.08 0.022 0.113 marbling 5.714 <0.0001 gene ATP1B2 ASGA0055076 12 26549245 42 90 45 177 1.325 0.408 -0.392 0.573 C18: 1 2.294 <0.05 gene ATP1B2 ASGA0055076 12 26549245 42 92 45 179 -1.144 0.3 -0.169 0.418 C18: 2 3.093 <0.005 gene ATP1B2 ALGA0066960 12 26575981 107 112 14 233 0.409 0.125 0.049 0.151 marbling 3.341 <0.01 gene WRAP53 ALGA0066960 12 26575981 86 82 11 179 -1.464 0.449 -0.471 0.546 C18: 2 2.743 <0.05 gene WRAP53 M1GA0016958 12 26581252 46 137 50 233 -0.205 0.091 0.267 0.119 marbling 2.077 <0.05 gene TP53 ASGA0055081 12 26595674 46 138 50 234 -0.205 0.091 0.26 0.119 marbling 2.03 <0.05 gene DNAH2 ALGA0066969 12 26671590 14 53 150 217 -0.284 0.175 -0.336 0.246 IMF 2.317 <0.05 gene GUCY2D ALGA0066969 12 26671590 14 53 150 217 -0.398 0.12 -0.273 0.168 marbling 6.227 <0.00001 gene GUCY2D ALGA0066969 12 26671590 11 43 112 166 -1.049 0.594 -1.002 0.825 C18: 1 2.303 <0.05 gene GUCY2D ALGA0066969 12 26671590 11 43 114 168 1.436 0.437 0.3 0.608 C18: 2 4.097 <0.001 gene GUCY2D ALGA0066974 12 26715540 14 68 151 233 -0.395 0.122 -0.105 0.161 marbling 4.65 <0.001 gene ALOX15B ALGA0066974 12 26715540 11 53 113 177 -1.044 0.603 -0.745 0.8 C18: 1 2.016 <0.05 gene ALOX15B ALGA0066974 12 26715540 11 53 115 179 1.427 0.439 -0.124 0.583 C18: 2 3.307 <0.01 gene ALOX15B ALGA0066986 12 26867891 23 105 105 233 0.558 0.148 0.206 0.193 IMF 5.239 <0.0001 gene NDEL1 ALGA0066986 12 26867891 23 105 105 233 0.453 0.092 0.408 0.121 marbling 12.892 <0.0000000005 gene NDEL1 ALGA0066986 12 26867891 21 71 85 177 1.485 0.449 1.097 0.627 C18: 1 4.765 <0.001 gene NDEL1 ALGA0066986 12 26867891 21 72 86 179 -1.094 0.334 -0.722 0.465 C18: 2 4.452 <0.001 gene NDEL1 H3GA0034753 12 26885737 36 123 74 233 -0.291 0.091 0.094 0.121 marbling 2.186 <0.05 gene NDEL1 ASGA0055114 12 26934636 132 83 19 234 0.349 0.109 0.041 0.146 marbling 2.875 <0.05 gene MYH10 ASGA0055114 12 26934636 97 66 16 179 -1.053 0.38 0.065 0.515 C18: 2 2.355 <0.05 gene MYH10 MARC0074335 12 26957184 19 82 132 233 -0.349 0.109 0.049 0.146 marbling 2.817 <0.05 gene MYH10 MARC0074335 12 26957184 16 66 97 179 1.053 0.38 0.065 0.515 C18: 2 2.355 <0.05 gene MYH10 ALGA0067003 12 26997723 74 124 36 234 0.291 0.091 0.087 0.121 marbling 2.19 <0.05 gene MYH10 DRGA0011818 12 27199873 17 110 92 219 -0.456 0.117 0.075 0.144 marbling 4.016 <0.001 gene NTN1 DRGA0011818 12 27199873 13 81 72 166 -1.287 0.584 -0.671 0.725 C18: 1 2.438 <0.05 gene NTN1 DRGA0011818 12 27199873 13 82 73 168 1.173 0.417 0.412 0.517 C18: 2 3.352 <0.01 gene NTN1 ALGA0067055 12 27305775 84 105 44 233 0.46 0.122 0.239 0.177 IMF 3.063 <0.005 gene NTN1 ALGA0067055 12 27305775 84 105 44 233 0.486 0.079 0.206 0.114 marbling 7.717 <0.000001 gene NTN1 ALGA0067055 12 27305775 67 76 34 177 1.279 0.4 -0.249 0.592 C18: 1 2.428 <0.05 gene NTN1 ALGA0067055 12 27305775 67 78 34 179 -1.164 0.292 0.199 0.428 C18: 2 3.69 <0.01 gene NTN1

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Single nucleotide polymorphism (SNP) markers associated with          unsaturated fatty acid in pig and their methods for evaluation <130> IPDB39803 <160> 42 <170> KopatentIn 2.0 <210> 1 <211> 121 <212> DNA <213> pig <400> 1 ctcctcagtt tgcagactga cccaggctgg ggggcagcag agggtgggga aaccctcccc 60 accaccacca ccacacacac tgtgctaaat cctgggtcca tctctgctag gaaaatggct 120 t 121 <210> 2 <211> 104 <212> DNA <213> pig <400> 2 acagcgactt ggagaaacag atgaagagaa gttatttgtg gaagcaggga aggctctatt 60 agccgaaggt gtcactttaa gacgagtagg agaagcaagg actc 104 <210> 3 <211> 121 <212> DNA <213> pig <400> 3 aaaagctgcc aattctttct ttgaggcagg cagcctgtgt gtgtgtggcg gggagggcac 60 tggcagagca gggcccactc tgcatacaca caggcagagt ccagatgtcc agtgtgcaac 120 c 121 <210> 4 <211> 121 <212> DNA <213> pig <400> 4 agaaaaagac agaaaggcaa aaaaaaaaaa aagaaagaaa gaaaatgtcc ccagacgttg 60 tcaaatgttc cacggccagt taccaccaat cccattgaga accaccaccc tagataatgt 120 c 121 <210> 5 <211> 121 <212> DNA <213> pig <400> 5 tcacatcctc acatatccca gtcaggttca ttaccactga gctccaaaga aacaaagaaa 60 taaaatttaa ttcatgtgca tggagatccc atagaaatga gacctaaaaa atgaccaaag 120 c 121 <210> 6 <211> 121 <212> DNA <213> pig <400> 6 acaaatgcaa gttgagttgc attttattct taacttagta ctttctgaga cagaatgcca 60 ttatacttat cctttaaaca tcccagttaa agacttgata tgactgaatt gaaactaaaa 120 g 121 <210> 7 <211> 121 <212> DNA <213> pig <400> 7 gcaatagaag cataagagat aagaggacaa ctgttggtga taataaggat ggaaaacagg 60 atttagcaac tgacggaatg tatcaagtaa gggcaccagc acgccagtga tctctgaaaa 120 c 121 <210> 8 <211> 121 <212> DNA <213> pig <400> 8 gacttaacat agtttggggt tttgccaggg cagtacaggt aggtatgtaa tcccttatct 60 acacttctga aatttaaaga ggtctgaaaa ttcatagtta tcttgttcgt ttgttttggt 120 a 121 <210> 9 <211> 121 <212> DNA <213> pig <400> 9 acatttagag tgctacctaa tgaatagtgt acatttaaat agcatgagcc ataatttgca 60 aaaagcatga aaggaaataa aagcttcaag tgtcagaatg ggattctggg gcagagcagc 120 a 121 <210> 10 <211> 121 <212> DNA <213> pig <400> 10 ggcacaggga tctgggtcaa ctaggacgaa gtggggaaaa gggtctccga cgcgttctga 60 tgacagcttc agacgggcag aagtttaggt tcagtctaag aaaatgcctg gcaggtccct 120 c 121 <210> 11 <211> 121 <212> DNA <213> pig <400> 11 ggaggaacga gaccagcgca cggccgctga gaaccgggag aaggagcaaa acaagaggct 60 acagcggcag ctccgagaca ccaaggagga gatgggcgag cttgccagga aggaggccga 120 g 121 <210> 12 <211> 121 <212> DNA <213> pig <400> 12 ttcttagtcg gatttgtttc cactgcacca tgacgggaac tccttgaagc ttattttaaa 60 tcactatcag aaataaattt atttgtactc acaggtattc ctgataatga gaatatttgt 120 t 121 <210> 13 <211> 121 <212> DNA <213> pig <400> 13 aagcgtgaag gaacttcagg ggatccaaaa gtggcccaga gggtgggata gggctggtca 60 tgcctcctgt gctcgggcag aacaacttcg aggagccagt ggcactgcag gagatggaca 120 c 121 <210> 14 <211> 121 <212> DNA <213> pig <400> 14 aaggagagtt aggttccctg ggtacgaaag cagctaaaca tgttatctac acagcaaata 60 tgggggcact tgtcaatcca gacactttat acacatacat ataacccaaa tggaatccgt 120 t 121 <210> 15 <211> 121 <212> DNA <213> pig <400> 15 ccaggaactc catatgccac ggggtagcca aaaaaaaagg cagttattta ccctcaagta 60 ataatggcag ctcagaccca gggccgcctc cagtgagaaa gacatctcac ttcaggaaat 120 g 121 <210> 16 <211> 121 <212> DNA <213> pig <400> 16 ggttttactc taagagtttt tactccaaaa taaagatggt actctcttag tacttgtgct 60 acacacccac ggatgcattc attagagaaa agcaggacag tcacaaaagg caaccacatt 120 c 121 <210> 17 <211> 121 <212> DNA <213> pig <400> 17 aacctcttcc tccaagcacc gaggcaccaa gacgttgccc atgactgcca cttaaagtgg 60 acaaaattag tttaggccca ttaagattta gatctttata tgtgaatatg caaagcaatt 120 t 121 <210> 18 <211> 121 <212> DNA <213> pig <400> 18 gctcatgcgg ctgctgtaag tgaggggtcg gcagatggcc aagaatcggt cataggccat 60 agctgtcaac aggaagcagt ctacaccaac caggagatgg aagaagaaaa gctgtgtgag 120 g 121 <210> 19 <211> 121 <212> DNA <213> pig <400> 19 ttggagttga aacttcccag atgctatatg gagaaatgca gtatagtctt actatcgaga 60 acatctacag gctggtagtc agcaactctg gagctggaga tgtcctcgaa ttcagtttca 120 t 121 <210> 20 <211> 121 <212> DNA <213> pig <400> 20 cctcctccgc ccgagccttc tgaccgttac cccttctctc cccgggccct gcygccaggc 60 tgggaagttc gacatcatcc ccacaatgac caccatcggc tctggcattg gcatcttcgg 120 g 121 <210> 21 <211> 121 <212> DNA <213> pig <400> 21 aagaaagtga cgaggtgatg tgtgcacctt tgtatcttca gcacacaaaa caaggcttag 60 aacagaagag ctcaggactt tgggcaggcg ttgggctaaa tgaatgagcg cccctctcaa 120 g 121 <210> 22 <211> 121 <212> DNA <213> pig <400> 22 ccacacgact gcagaagaca tctgtcacac accaaccctg ccccgtgtcc gggccccacg 60 tgaaagggaa gacgctctca ctggtgcttg gctgcatagt ttagttttga ttgcagttct 120 t 121 <210> 23 <211> 121 <212> DNA <213> pig <400> 23 ttctgcccga gtgctctacg tcctccagca gcaggtgcgg gattcaggga gggccccccc 60 accttgtttt tgtcttctca gggaattcct gttttctttc tgtctcaggg atgccagcac 120 c 121 <210> 24 <211> 121 <212> DNA <213> pig <400> 24 attagacatt aatcgcatta actgaccagg ggaaaaaagc atcatttggt ggctatgctt 60 atgtaaaaca ctaagccata gcacgcctgg tctgcagagc tacacgtagg ctttcagctt 120 c 121 <210> 25 <211> 121 <212> DNA <213> pig <400> 25 gctcccagga gcaggcccag gtccacgctg gcttgccctc tcagcgctgg gggccaggcc 60 atgagtgtct ggctttctgg gcaggctctg aagggttatc tccctgcttc tgttctccag 120 t 121 <210> 26 <211> 121 <212> DNA <213> pig <400> 26 tggactgagc caggaccttg tcaggactcg actgagctct gacctcagct agggccctta 60 tccaacattc agttctcccg ggagaccctg cagaccagta gtcctggggg gagacgctgc 120 a 121 <210> 27 <211> 121 <212> DNA <213> pig <400> 27 tttgtttaat ttaatgccaa ttatacacat tttgaagaag ccctagaaaa atttccagcc 60 aaggactggg aagccttgac ccagcacaag cttagaagca aaaattgctt gaaagagtaa 120 t 121 <210> 28 <211> 90 <212> DNA <213> pig <400> 28 ccagtggaag gaggtkgaca tgcaggaaaa agttatctgg gaaatgagaa tggggcagct 60 agcacccggg agcacaccac cccctctcac 90 <210> 29 <211> 121 <212> DNA <213> pig <400> 29 cagcggcacc gacgaccccg ccttcgtcga ggcagccaac accttgaagt attcgtttta 60 tggccagagg aggtgtcttt ccttcacgcc gtagctacga agttgtgtga cccggggctg 120 a 121 <210> 30 <211> 121 <212> DNA <213> pig <400> 30 cctttgttgg ctaggcctga tgattcgtcc taagactgag aaccttgatg tcatcgtcaa 60 tgtcagtgac actgaaagct gggaccagca tgttcagaag ctcaacaagt tcttagagcg 120 t 121 <210> 31 <211> 121 <212> DNA <213> pig <400> 31 ggctcacctc tgccacaggt ctgctcagct caagcccaac actcgcaaga tacaggtcta 60 tccaggcttc tctctcctac tcctagggcc cctgatggtt cctgcatcct gaccaatagt 120 g 121 <210> 32 <211> 121 <212> DNA <213> pig <400> 32 gtgaaagcag ggtgcccgtt cctggggtgg tcctgaaccc ccgggccaat gggcagctct 60 atgagatggg aagctgcggg catgcgagtt ctctgtgcaa cctgcaaggc actcctagcc 120 c 121 <210> 33 <211> 121 <212> DNA <213> pig <400> 33 agtggctggg gacacgtctc tgcttcccaa aaccatatgg cagccggtct cccaacgttc 60 aaaagctggc agagtgcgtg tagttggagg ctccagctct gctcagggcg gtggtaaaag 120 g 121 <210> 34 <211> 121 <212> DNA <213> pig <400> 34 cgggctgatc agggcagtgt ggcagcagcc tgaaaggcag acctgcagct gcttttatgt 60 agggcttgcc atggggggct tgggacgctc ggaggggaaa gaagctgcct ggctggctgc 120 a 121 <210> 35 <211> 121 <212> DNA <213> pig <400> 35 ggctcaggcc aacagccatc acagaagcca gctcctcaga aaggagccac ctggggttga 60 tagatgccaa agtgtgaaca tggctttgac ccacagctgc aggggggagg agccctggga 120 g 121 <210> 36 <211> 121 <212> DNA <213> pig <400> 36 tcccttatac acagtcttgg ttaattttta tgcactgtaa aactgttgca aggttttctt 60 tctgaaaatg tgtaagcttg gttatattgt gtttaaaatc cagtgggcag atgtaaattg 120 t 121 <210> 37 <211> 121 <212> DNA <213> pig <400> 37 gtaagaatta aatgtttttg tgagctggcc ttttctcagg tgtttaggtt atatggtaac 60 tggattgtat agacccactt aaacactaac ctgttattgc tggagttgca ttctctggtg 120 g 121 <210> 38 <211> 121 <212> DNA <213> pig <400> 38 tgcgtggcgg gttctcagtg ctcatttaca cgggtggcga cacttatgct ctaaggtaca 60 atctcaacag ctaaaaatga ttctggaaca aacattcaac taccatgaat tcccagaagc 120 a 121 <210> 39 <211> 97 <212> DNA <213> pig <400> 39 cttgttagat actcttcagt gagagaaata cattgtttct atgctctcat gtgcagaaga 60 aatcagattt ttcctgttca ttcagaacta tatttta 97 <210> 40 <211> 121 <212> DNA <213> pig <400> 40 tttttcttac ttactctaaa tacgaaccct tgcgccccta aagtctcctg gcctgagcaa 60 tgtcttgaga gctccactta gcacgttttt agggcttttc tgaactagat cacacattga 120 a 121 <210> 41 <211> 121 <212> DNA <213> pig <400> 41 ttgcgcctct gtagtgacct gagctgctgc ggtcagattc ttaacctgtt gcaccataac 60 aggaattcct tagtttgttt tcttttaaaa aatgtatatg tgcttactta ggaaggtttg 120 g 121 <210> 42 <211> 121 <212> DNA <213> pig <400> 42 gggctgcatt tagtgggaat gctgataaag caccgcctta tggccagagg aagctaaccc 60 aggtagcgat ggcatagtac caatgaccac agaagcagag atgttgtccc tctaagaagg 120 g 121

Claims (12)

Separating DNA from pigs; And
Any one selected from the group consisting of SEQ ID NOs: 1 to 27 and 29 to 42 having any single base mutation selected from the group consisting of A↔C, A↔G, T↔C, and T↔G in SEQ ID NO: 61 Amplifying the separated DNA using a primer pair capable of amplifying the amino acid sequence of, characterized in that it comprises the step of amplifying, the information providing method for determining the unsaturated fatty acid content of the pig.
The method of claim 1,
And the unsaturated fatty acid is oleic acid or linoleic acid.
Separating DNA from pigs; And
Any one selected from the group consisting of SEQ ID NOs: 1 to 27 and 29 to 42 having any single base mutation selected from the group consisting of A↔C, A↔G, T↔C, and T↔G in SEQ ID NO: 61 Amplifying the separated DNA using a primer pair capable of amplifying the amino acid sequence of the, characterized in that, comprising the steps of amplifying, the information providing method for identifying the pork meat.
Any one selected from the group consisting of SEQ ID NOs: 1 to 27 and 29 to 42 having any single base mutation selected from the group consisting of A↔C, A↔G, T↔C, and T↔G in SEQ ID NO: 61 Kit for identifying the unsaturated fatty acid content or meat quality of pigs comprising a primer pair capable of amplifying the amino acid sequence of.
Any one selected from the group consisting of SEQ ID NOs: 1 to 27 and 29 to 42 having any single base mutation selected from the group consisting of A↔C, A↔G, T↔C, and T↔G in SEQ ID NO: 61 Monobasic polymorphism (SNP) marker for identifying the unsaturated fatty acid content or meat quality of pigs, characterized in that it comprises the amino acid sequence of.
The method of claim 5,
Unsaturated fatty acid is monobasic polymorphism (SNP) marker for identifying the fatty acid content or meat quality of pigs, characterized in that oleic acid or linoleic acid.
Separating DNA from pigs; And
Amplifying the isolated DNA using a primer pair capable of amplifying the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30, the unsaturation of pigs Informational method for determining fatty acid content.
The method of claim 7, wherein
And the unsaturated fatty acid is oleic acid or linoleic acid.
Separating DNA from pigs; And
Amplifying the separated DNA using a primer pair capable of amplifying the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30; Information how to check the meat quality.
Kit for identifying the unsaturated fatty acid content or high quality of pigs comprising a primer pair capable of amplifying the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30.
Single nucleotide polymorphism (SNP) marker for unsaturated fatty acid content or meat quality of pigs, characterized in that it comprises the amino acid sequence of SEQ ID NO: 28 having a single base mutation of A↔G in the nucleotide sequence 30th.
The method of claim 11,
Unsaturated fatty acid is monobasic polymorphism (SNP) marker for identifying the fatty acid content or meat quality of pigs, characterized in that oleic acid or linoleic acid.