KR20120063267A - Composition for antiaging comprising the extract of decaspermum fruticosum as an active ingredient - Google Patents

Abstract

PURPOSE: A composition containing Decaspermum fruticosum extract is provided to suppress DPPH free radical generation and to ensure excellent antioxidation. CONSTITUTION: A pharmaceutical composition for anti-aging contains Decaspermum fruticosum extract as an active ingredient. The extract is prepared using water, low alcohol of C1-C4, or a mixture thereof. A health food composition for anti-aging contains the extract as an active ingredient. A cosmetic composition for anti-aging contains the extract as an active ingredient.

Description

Composition for antiaging comprising the extract of Decaspermum fruticosum as an active ingredient

The present invention is Decaspermum fruticosum ) relates to an anti-aging composition containing the extract as an active ingredient.

Free radicals that cause aging and disease in the body are formed by biological reactions during normal metabolic processes in the human body, and are known to cause various diseases by causing harmful toxicity to cells and tissues (Folin O & Denis W, 1912, J Biol Chem 12: 239-249). The physiological action of inhibiting these harmful free radicals is similar to that of an electron donating ability and a superoxide dismutase which inhibits oxidation by donating electrons to oxidative free radicals. It converts superoxide anion radicals into normal oxygen.

Many bioactive ingredients with strong antioxidant effects contribute greatly to the prevention of ulcers and spasms caused by oxidative stress, control of gastric juice secretion and diarrhea, prevention of diabetes, prevention of cancer, heart disease and degenerative diseases. Azuma K et al., 1999, J Agric Food Chem 47: 3963-3966, Ham SS et al., 1997, J Food Sci Nutr 2: 155-161. In recent years, as interest in health has increased, a resource containing a large amount of functional substances, such as anti-cancer, anti-allergy, anti-obesity, antioxidant, and antibacterial effects, from various plant resources is selected, and thus, medicines, food additives, or cosmetics are used as raw materials. Is being developed worldwide (Shin DB, et al., 2006, Food Sci Biotechnol., 15: 357-362, Park et al., 2004, Korean J Nutr, 37: 364-372, Kim). et al., 2006, Kor J Food Sci. Technol. 38: 799-804, Ali KA et al., 2005, Food Chem., 89: 27-36).

Superoxide dismutase, an antioxidant, breaks down free radicals, or superoxide radicals, released during the respiration of cells into hydrogen peroxide and oxygen. When hydrogen peroxide reacts with metal ions such as iron or copper ions, it generates active oxygen called stronger hydroxy radicals. The antioxidants catalase and glutathione peroxidase, which remove hydrogen peroxide before it reacts with metal ions, decompose hydrogen peroxide into water and carbon dioxide. If an antioxidant that removes hydrogen peroxide does not completely remove 5% of hydrogen peroxide, it reacts with metal ions in the body and turns into hydroxy radicals, causing our bodies to age and die. Frequent exposure to radiation or ultraviolet light converts hydrogen peroxide into hydroxy radicals to promote aging, and there is a method of taking antioxidants to delay this.

Superoxide radicals (Superoxide radical, O ^{2 -)} itself is reactive is relatively weak, but easily is converted to H ₂ O ₂ As a result, generation of hydroxyl radicals strong reactivity, or age trick oxides (nitric oxide, NO) and the reaction the reactivity is strongest peroxy nitrite ^- causes, such as the generated by nitrification of the oxide, the protein tyrosine SH- group (group) (nitration), lipid peroxidation, DNA damage (peroxy nitrite, ONOO).

Decasperm fruiticosum is a short shrub, with leaves facing each other. Flowers are pale pink, fruits are purple, and have many seeds. Native to Burma and native to Australia and Pacific Islands, it grows in volcanic soils. It has been reported that cosmetics, including many plant extracts, in addition to decasperm fructosum, can improve skin by giving shine and elasticity to dry skin (Ohara, Mitsuharu & Hori, Michimasa, JP 2001220312). It has also been reported that the young leaves and flower ear extracts of the plant blacken the hair and help it grow, but the antioxidant effect, whitening and wrinkle improvement have not been studied.

Antioxidants are widely distributed in copper and plants. Antioxidants such as phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium in fruits and vegetables can delay or prevent the oxidation of fats, and cancer, cardiovascular diseases, etc. By preventing or delaying it plays an important role in preventing aging. Tocopherols and vitamin C, which are natural antioxidants present in the plant kingdom, are widely used in food, medicine, and cosmetics. The rancidity of fats and oils or foods containing oils and fats is mainly caused by the combination with oxygen in the air. To prevent this, many synthetic or natural antioxidants have been developed. Synthetic antioxidants include Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate. Synthetic antioxidants are widely used in commercial foods because of their excellent antioxidant power and low price, but their usage is legally regulated due to concerns about stability, such as human side effects. Natural antioxidants, such as tocopherol, are safe for the human body, but have low disadvantages in preventing oxidative chain reactions and are expensive. Therefore, in recent years, researches are being actively conducted to develop safer and more natural antioxidants.

Accordingly, the inventors of the present invention, while screening plants excellent in antioxidant effects, Decaspermum fruity comsum ( Decaspermum fruticosum ) by confirming that the extract exhibits a strong antioxidant activity, the present invention was completed by revealing that it can be usefully used as an active ingredient of the anti-aging composition.

An object of the present invention to provide an anti-aging pharmaceutical composition containing an extract of decasperm fruiticumsum as an active ingredient.

Another object of the present invention to provide an anti-aging health food composition containing the extract of decasperm fruiticumsum as an active ingredient.

Still another object of the present invention is to provide an anti-aging cosmetic composition containing an extract of decasperm fruiticumsum as an active ingredient.

In order to achieve the above object, the present invention provides an anti-aging pharmaceutical composition containing an extract of decasperm fruiticumsum as an active ingredient.

The present invention also provides an anti-aging health food composition containing the extract of decasperm fruiticumsum as an active ingredient.

Furthermore, the present invention provides an anti-aging cosmetic composition containing an extract of decasperm fruiticumsum as an active ingredient.

The extract extracted from the decasperm fruiticumsum of the present invention shows an inhibitory effect of DPPH free radical production, ABTS free radical production and superoxide radical inhibition effect in a concentration-dependent manner to confirm that it has an excellent antioxidant effect, anti-aging drug It can be usefully used as a raw material for health food or cosmetics.

1 is a measurement result for ABTS scavenging activity of the decasperm fruity comsum extract according to an embodiment of the present invention.
2 is a measurement result of the superoxide dismutase (SOD) activity of the decasperm fruity comsum extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides an anti-aging pharmaceutical composition containing decasperm fruiticum extract as an active ingredient.

In another aspect, the present invention provides a pharmaceutical composition for antioxidant containing decasperm fruiticum extract as an active ingredient.

In the pharmaceutical composition according to the present invention, the extract is preferably used to extract the decasperm fruiticum dry dried water, C ₁ ~ C ₄ lower alcohol or a mixed solvent thereof.

The decasperm fruiticum used in the present invention may use all of its roots, stems, and leaf parts, but may preferably use stem parts.

In addition, it is preferable to use methanol or ethanol as the lower alcohol of C ₁ to C ₄ , and more preferably to use methanol.

Hereinafter, the method of extracting the decasperm fruiticum extract according to the present invention will be described in detail.

The decasperm fruiticum extract can be prepared by a preparation method comprising the following steps:

Extracting by adding an extraction solvent to decasperm fruiticum (step 1);

Cooling the extract of step 1 and filtering (step 2); And

Concentrating the filtered extract of step 1 under reduced pressure and drying (step 3).

In the above method, the decasperm fruiticum of step 1 may be used without limitation, such as cultivated or commercially available, and all of its white, stem, and leaf parts may be used, but preferably, stem parts may be used. have.

The extraction solvent of step 1 is preferably water, C ₁ ~ C ₄ lower alcohol or a mixture thereof, the lower alcohol is preferably used ethanol or methanol.

As extraction methods, conventional methods in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction, can be used, and the extraction is preferably performed once to five times by hot water extraction, and three times to extract repeatedly. More preferably, but is not limited thereto.

The extraction solvent may be added 1 to 10 times the weight of the dried decasperm fructosumum, it is preferable to add 2-3 times.

Extraction temperature is preferably 20 to 100 ℃ but is not limited thereto. In addition, the extraction time is preferably 10 to 48 hours, preferably 15 to 30 hours, but is not limited thereto.

In the above method, the vacuum concentration concentrator of step 3 may use a vacuum vacuum concentrator or vacuum rotary evaporator, and the drying may be vacuum drying, vacuum drying, boiling drying, spray drying or freeze drying.

In order to confirm the antioxidant effect of the decasperm fruiticumsum extract, the present inventors investigated the effect of DPPH free radical scavenging on 1,1-diphenyl-2-picryl-hydrazyl (1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma) method showed that decasperm fruity cosum extract extracts 24.3 ± 5.09, 36.9 ± 5.38, 58.4 ± 6.24, 70.7 ± 8.79, 79.2 at concentrations of 2.5, 5, 10, 20, 40 and 80 μg / ml, respectively. It was confirmed to exhibit significant DPPH free radical scavenging effects of ± 9.76 and 88.7 ± 4.18% (see Table 1).

In addition, the present inventors measured the ABTS free radical scavenging effect according to various concentrations of decasperm fruity comosum extract, in order to confirm the antioxidant effect of the decasperm fruity comosum extract, each decasperm fruity como extract 2.5, It was confirmed that they exhibit significant ABTS free radical scavenging effects of 12, 28, 40, 70, 97 and 99% at 5, 10, 20, 40 and 80 μg / ml concentrations (see FIG. 2).

In addition, the present inventors measured the superoxide radical scavenging effect of each concentration of decasperm fruity comsum extract by using a SOD kit, in order to confirm the SOD (Superoxide Dismutase) activity of the decasperm fruity comum extract, decasperm Fruiticcosum extracts were found to exhibit significant vesicular oxide radical scavenging effects of 16, 34, 48, 59 and 68% at 2.5, 5, 10, 20 and 40 μg / ml concentrations (see FIG. 3).

Therefore, the decasperm fruiticum extract of the present invention exhibits a DPPH free radical scavenging effect and an ABTS free radical scavenging effect in a concentration-dependent manner for antioxidant, and has a significant superoxide radical scavenging effect, thus having an excellent antioxidant effect, thus preventing the aging of the pharmaceutical composition. It can be usefully used as an active ingredient of a health food composition and a cosmetic composition.

When the composition of the present invention is used in medicine, it may contain at least one active ingredient exhibiting the same or similar function in addition to the decasperm fruiticum extract.

The composition of the present invention may be administered in various oral and parenteral dosage forms during actual clinical administration, and when formulated, diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc., which are commonly used, may be used. Are prepared using. Solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, which solid preparations contain at least one excipient such as starch, calcium carbonate, gelatin, etc. Mix and quiet. Liquid preparations for oral administration include suspensions, solutions, emulsions, and syrups, and may include various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, and lyophilized preparations. As the non-aqueous solvent and suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used.

Dosage varies depending on the weight, age, sex, health condition, diet, time of administration, method of administration, rate of excretion and severity of the patient. The daily dosage of the decasperm fruiticum extract according to the present invention is about 0.1 to 100 mg / kg, preferably 5 to 50 mg / kg, more preferably administered once to several times a day.

The present invention also provides an anti-aging health food composition containing the decasperm fruiticum extract as an active ingredient.

Furthermore, the present invention provides a health food composition for antioxidant containing decasperm fruiticum extract as an active ingredient.

In the health food composition according to the present invention, the extract may be used by the extraction method described above.

The composition according to the present invention can be added to the dietary supplements such as decasperm fruity comsum extract for foods, beverages for the purpose of antioxidant activity.

There is no particular limitation on the kind of the food. Examples of the foods to which the above substances can be added include dairy products including dairy products, meat, sausage, bread, biscuits, rice cakes, chocolate, candies, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, Beverages, alcoholic beverages and vitamin complexes, dairy products, and dairy products, all of which include health functional foods in a conventional sense.

The decasperm fruiticum extract of the present invention can be added as it is to food or used with other foods or food ingredients, and can be suitably used according to conventional methods. The amount of the active ingredient to be mixed can be suitably determined according to its use purpose (for prevention or improvement). In general, the amount of the extract in the health food can be added to 0.1 to 90 parts by weight of the total food weight. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

The health functional beverage composition of the present invention has no particular limitation on the other ingredients other than the above-mentioned extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally from about 1 to 20 g, preferably from about 5 to 12 g per 100 compositions of the present invention.

In addition to the above, the decasperm fruiticum extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors such as flavoring agents, coloring and neutralizing agents (cheese, chocolate, etc.), pectic acid and its Salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like. In addition, the decasperm fruiticum extract of the present invention may contain fruit flesh for the production of natural fruit juice and fruit juice beverage and vegetable beverage.

These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of decasperm fruiticum extract.

The present invention also provides a cosmetic composition for preventing aging containing decasperm fruiticum extract as an active ingredient.

Furthermore, the present invention provides a cosmetic composition for antioxidant containing decasperm fruiticum extract as an active ingredient.

In the cosmetic composition according to the present invention, the extract may be used by the above extraction method.

The decasperm fruiticum extract according to the present invention can be used in an antioxidant cosmetic composition, wherein the antioxidant cosmetics include, but are not limited to, lotions, ointments, gels, creams, patches or sprays. In the preparation of the antioxidant cosmetics containing the decasperm fruity comsum extract of the present invention, the skin decasperm fruity extract of the present invention is usually contained in the composition 1 to 15 parts by weight, preferably 2 or 10 parts by weight may be added. In addition to the decasperm fruiticum extract of the present invention, the skin external preparation of the present invention contains fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, and fragrances. For surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients commonly used in external preparations. The ingredients may also be introduced in amounts generally used in the field of dermatology.

Hereinafter, an Example demonstrates this invention.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

< Example  1> Decaspermium Fruiticosum  Preparation of Extract

20 mg of decasperm fruity comsum methanol extract was purchased from the Overseas Biomaterials Herb Center in Korea Research Institute of Bioscience and Biotechnology.

< Experimental Example  1> Decaspermium Fruiticosum  Extract DPPH  freedom Radical  Scavenging activity measurement

Free radical scavenging activity of 1,1-diphenyl-2-picryl-hydrazyl (1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma) with respect to the decasperm fruiticumsum extract obtained in Example 1 It was measured by the method. To measure the free radical scavenging activity, 90 μl of 0.15 mM DPPH solution dissolved in ethanol was placed in a 96-well plate, and the extracts were dispensed at concentrations of 2.5, 5 10, 20, 40, and 80 μg / ml, respectively. After left to stand, the absorbance was measured at 517 nm with a microplate meter. The free radical scavenging activity (%) is calculated by the following Equation 1 and is shown in Table 1 below. At this time, the group treated with DMSO was used as a negative control group, and the group treated with BHA, a synthetic antioxidant, was used as a positive control group.

(In Equation 1,

A: absorbance of the negative control group not treated with the extract of the present invention

B: The absorbance of the experimental group treated with the extract of the present invention.)

Extract (μg / mL) Free radical scavenging ability (%) Decasperium Fruiticosum Extract 2.5 24.3 ± 5.09 Decasperium Fruiticosum Extract 5 36.9 ± 5.38 Decasperium Fruiticosum Extract 10 58.4 ± 6.24 Decasperium Fruiticosum Extract 20 70.7 ± 8.79 Decasperium Fruiticosum Extract 40 79.2 ± 9.76 Decasperium Fruiticosum Extract 80 88.7 ± 4.18 BHA 2.25 15.9 ± 0.63 BHA 4.5 31.5 ± 0.63 BHA 9 53.0 ± 0.97 BHA 18 68.9 ± 2.15 BHA 36 80.7 ± 1.22

As shown in Table 1, as a result of DPPH radical scavenging activity, when the decasperm fruity comsum extract of the present invention was dispensed at a concentration of 2.5 μg / ml to 80 μg / ml, DPPH radical scavenging activity was concentration-dependently 19.21 to 92.88. Excellent activity of% was shown. In the case of positive control group BHA, DPPH radical scavenging activity also showed a concentration-dependent activity of 15.27 to 81.92% when dispensed at a concentration of 2.25 μg / ml to 36 μg / ml.

Therefore, it was confirmed that the decasperm fruiticum extract of the present invention effectively exhibited free radical scavenging activity equal to or higher than that of BHA, a positive control group.

< Experimental Example  2> Decaspermium Fruiticosum  Extract ABTS  freedom Radical  Scavenging activity measurement

ABTS radical scavenging activity was mixed with ABTS and potassium persulfate to a final concentration of 7 mM and 2.45 mM, respectively, and left in the dark for 12 hours to form ABTS radicals. It was diluted with distilled water so that the absorbance was 0.7 at 734 nm, and the plate was dispensed into 96-well plates, and the samples were dispensed at concentrations of 2.5, 5, 10, 20, 40, and 80 μg / ml, and then in the dark at room temperature. The reaction was carried out for 7 minutes. Absorbance was measured using a microplate meter at 734 nm. At this time, the group treated with DMSO was used as a negative control group, and the group treated with BHA, a synthetic antioxidant, was used as a positive control group.

As a result, as shown in FIG. 2, it was confirmed that decasperm fruiticum extract had ABTS radical scavenging activity in a concentration-dependent manner.

< Experimental Example  3> Decaspermium Fruiticosum  Extract SOD  Active measurement

In order to confirm the antioxidant activity of the extract obtained in Example 1, the scavenging activity of the superoxide radical acting as a precursor of other harmful active oxygen species was measured. The superoxide radical scavenging activity can be searched by measuring the activity of superoxide dismutase (SOD), an enzyme that eliminates superoxide by dismutating the superoxide. In the present invention, the SOD assay kit of Dojindo Was used. Specifically, a sample of each concentration is dispensed into a 96-well plate, and the water-soluble WST-1 (2- (4-indophenyl) -3- (4-nitrophenyl) -5- (2,4) reacts with a superoxide radical. -Dysulfophenyl) -2H-tetrazolium) was added, the SOD enzyme solution was added and reacted at 37 ° C. for 20 minutes, and the absorbance of the reaction solution was measured at 450 nm using a microplate meter. The superoxide radical scavenging activity of each sample was calculated by reducing reduction of WST relative to the control group, and the concentration of the sample for 50% scavenging of the superoxide radical was set to IC ₅₀ . At this time, the group treated with DMSO was used as a negative control group, and the group treated with BHA, a synthetic antioxidant, was used as a positive control group.

As a result, as shown in Figure 2 decasperm fruiticum extract was found to exhibit a superoxide radical scavenging ability in a concentration-dependent manner. In addition, the IC ₅₀ value was 12.832 ± 0.087 for the decasperm fruiticum extract and 60.519 ± 0.066 for the positive control BHA, indicating that the activity of the extract was better.

Therefore, the composition containing the decasperm fruity comsum extract according to the present invention as an active ingredient exhibits excellent radical scavenging activity and can be usefully used in medicine, health food, cosmetics, and the like.

< Formulation example  1> Preparation of Pharmaceutical Formulations

1. Preparation of tablets

Tablets containing the decasperm fruiticum extract of the present invention as an active ingredient were prepared by the following method.

The compounds of the above examples were sieved and mixed with lactose, starch and pregelatinized corn starch, then a suitable volume of purified water was added and granulated into a powder. The granules were dried and then mixed with magnesium stearate and compressed to obtain tablets.

The components of the tablet are as follows.

Decasperm Fruiticosum Extract 5.0 mg

Lactose BP 150.0 mg

Starch BP 30.0 mg

Pregelatinized Corn Starch BP 15.0 mg

1.0 mg magnesium stearate

2. Preparation of Capsule

Capsules containing the decasperm fruity comsum extract of the present invention as an active ingredient were prepared by the following method.

The compound of the above example was mixed with an excipient and magnesium stearate. The resulting mixture was filled into gelatin capsules to obtain capsules.

The components of the capsule are as follows.

Decasperm Fruiticosum Extract 5.0 mg

Starch 1500 100.0 mg

Magnesium Stearate BP 1.0 mg

3. Preparation of Injection

Injection solution containing the decasperm fruiticum extract of the present invention as an active ingredient was prepared by the following method.

Dissolve the decasperm fruiticum extract in an appropriate volume of sodium chloride BP for injection, adjust the pH of the resulting solution to pH 3.5 with dilute hydrochloric acid BP, and then adjust the volume with sodium chloride BP for injection and sufficiently Mixed. The solution was filled into a 5 ml Type I ampoule made of transparent glass, and the glass was dissolved and enclosed under an upper grid of air, followed by autoclaving at 120 ° C. for at least 15 minutes to obtain an injection solution.

The components of the injection solution are as follows.

Decasperm Fruiticum Extract 100 ㎍ / ml

Dilute hydrochloric acid BP until pH 3.5

Injectable sodium chloride BP up to 1 ml

< Formulation example  2> Decaspermium Fruiticosum  Preparation of Functional Foods Containing Extracts

The present inventors prepared the food containing the decasperm fruiticum extract as an active ingredient as follows.

1. Manufacture of beverage

522 mg of honey

Chioctosanamide 5 mg

Nicotinamide 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Decasperm Fruiticosum Extract 0.48? 1.28 mg

200 ml of water

A beverage was prepared using the above-mentioned composition and content by a conventional method.

2. Of chewing gum  Produce

Gum base 20%

Sugar 76.36? 76.76%

Decasperm fruiticum extract 0.24? 0.64%

1% fruit flavor

Water 2%

Chewing gum was prepared using conventional methods using the above composition and content.

3. Manufacture of Candy

50 sugar 60%

Starch syrup 39.26? 49.66%

Decasperm fruiticum extract 0.24? 0.64%

Orange flavor 0.1%

The composition and the content of the candy were prepared using a conventional method.

4. Biscuit  Produce

Force Class 1 88 kg

Gravity First Class 76.4 ㎏

16.5 kg per white

2.5 kg of salt

2.7 kg of glucose

Palm shortening 40.5 kg

5.3 kg of ammo

Medium kg 0.6 kg

0.55 kg sodium bisulfite

Rice flour 5.0 kg

Vitamin B1 0.003 kg

0.003 kg of vitamin B2

Milk Flavor 0.16 ㎏

71.1 kg of water

Whole milk powder 4 kg

Substitute powder 1 kg

0.1 kg of calcium phosphate

Spray salt 1 kg

25 kg of spray oil

Decasperm Fruiticosum Extract 0.2? 0.5 kg

Biscuits were prepared using conventional methods with the above composition and content.

5. Manufacture of Ice Cream

Milkfat 10.0%

Non-fat solids 10.8%

Sugar 12.0%

Starch syrup 3.0%

Emulsifying stabilizer (span) 0.5%

Spices (Strawberries) 0.15%

Water 63.31? 62.91%

Decasperm fruiticum extract 0.24? 0.64%

Ice cream was prepared using conventional methods using the above composition and content.

6. Chocolate  Produce

Sugar 34.36? 34.76%

Cocoa Butter 34%

Cocoa Mass 15%

Cocoa Powder 15%

Lecithin 0.5%

0.5% vanilla

Decasperm fruiticum extract 0.24? 0.64%

With the above composition and content, chocolate was prepared using conventional methods.

< Formulation example  3> Preparation of external preparation for skin

1. Preparation of Cream

Cetostearyl alcohol 2.8 parts by weight

2.6 parts by weight of beeswax

Stearic acid 1.4 parts by weight

2 parts by weight of glycerol phosphate monostearate

Fiji-100 Stearate 1 part by weight

Sesquioleate sorbitan 1.4 parts by weight

4 parts by weight of jojoba oil

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

2 parts by weight of macadamia oil

Tocopherol acetate 0.2 parts by weight

0.4 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methyl paraben 0.05 parts by weight

Glycerin 6 parts by weight

0.2 parts by weight of d-pandenol

4.6 parts by weight of decasperm fruiticum extract

0.2 parts by weight of triethanolamine

pt 41891 0.2 parts by weight

p-H2O 46.05 parts by weight

2. Preparation of Lotion

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

Allantoin 0.1 part by weight

Decasperm fruiticum extract 3.5 parts by weight

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

p-H20 48.3 parts by weight

3. Manufacture of Shampoo

The shampoo composition of the present invention was prepared using the following formulation.

Sodium Lauret-7-Sulfate 16 parts by weight

Lauroyl diethanolamide 2.0 parts by weight

Decasperm fruiticum extract 0.5 part by weight

DI water 81.5 parts by weight

4. Preparation of Rinse

The rinse composition of the present invention was prepared using the following formulation.

Bariku Art 638 2.0 parts by weight

Lauroyl diethanolamide 2.0 parts by weight

0.5 parts by weight of silicone oil (molecular weight of 10,000)

Decasperm fruiticum extract 0.5 part by weight

DI water 95.0 parts by weight

5. Preparation of Hair Oil

The oil composition for hair of the present invention was prepared using the following formulation.

40.0 parts by weight of octamethyl cyclotetrasiloxane

Decasperm Fruiticosum Extract 5.0 parts by weight

55.0 parts by weight of anhydrous ethanol

6. Manufacture of hair spray

The hair spray composition of the present invention was prepared as follows.

Decasperm Fruiticosum Extract 5.0 parts by weight

95.0 parts by weight of ethyl alcohol

Claims (9)

Anti-aging pharmaceutical composition containing decasperm fruiticum extract as an active ingredient.
The anti-aging pharmaceutical composition according to claim 1, wherein the decasperm fruiticum extract is extracted using water, a lower alcohol of C ₁ to C ₄ or a mixture thereof as a solvent.
3. The anti-aging pharmaceutical composition according to claim 2, wherein the lower alcohol is methanol or ethanol.
Anti-aging health food composition containing decasperm fruiticum extract as an active ingredient.
The anti-aging health food composition according to claim 4, wherein the decasperm fruiticum extract is extracted using water, a lower alcohol of C ₁ to C ₄ or a mixture thereof as a solvent.
6. The anti-aging health food composition according to claim 5, wherein the lower alcohol is methanol or ethanol.
Anti-aging cosmetic composition containing decasperm fruiticum extract as an active ingredient.
The anti-aging cosmetic composition according to claim 7, wherein the decasperm fruiticum extract is extracted using water, a lower alcohol of C ₁ to C ₄ or a mixture thereof as a solvent.
The anti-aging cosmetic composition according to claim 8, wherein the lower alcohol is methanol or ethanol.

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