KR20120021353A - Composition of external application for using bamboo sprout - Google Patents

Abstract

PURPOSE: An external use skin composition using bamboo shoot extract is provided to improve whitening and skin moisturizing and to treat acne and atopic dermatitis. CONSTITUTION: An external use skin composition for skin whitening contains bamboo shoot powder or extract as an active ingredient. An external use skin composition for treating atopic dermatitis contains the bamboo shoot powder or extract as an active ingredient. The bamboo extract is prepared by dipping bamboo shoots in a mixture solvent of water and ethanol. The composition is a cosmetic, soap, or pharmaceutical composition.

Description

Composition for external application using bamboo shoots {Composition of External Application for Using Bamboo Sprout}

The present invention relates to a skin external preparation composition using bamboo shoots, and more particularly, to a skin external preparation composition using bamboo shoots having skin skin whitening activity, skin moisturizing activity, acne improving activity, and atopic dermatitis improving activity.

Bamboo shoots refer to the young shoots sprouting from the bamboo stalk, which has all the traits found in the grown bamboo.

Bamboo shoots such as kings, swabs, and bamboo shoots are used for food, but the quality of bamboo shoots is the best.

Bamboo shoots are known to be effective in hypertension, arteriosclerosis, etc., because they contain proteins, vitamins B and C, and minerals. They are also known to be effective in constipation.

According to the agreement, bamboo shoots are sweet and slightly cold, which makes the body's fluids run smoothly and promotes restorative recovery.In the Korean Herbal Paintings, bamboo shoots lower blood pressure, sunburn dermatitis and chronic bronchitis. It is said to be effective in treating insomnia.

The present invention discloses the skin whitening activity, skin moisturizing activity, acne improving activity, atopic dermatitis improving activity of bamboo shoots.

An object of the present invention is to provide an external composition for skin using bamboo shoots.

Other and specific objects of the present invention will be presented below.

The present invention, as identified in the Examples and Experimental Examples below, the bamboo powder with the 70% ethanol extract of bamboo shoots have a melanin production inhibitory activity in B16F10 melanoma cells, acne gyunin propynyl sludge arc Ness (Propionibacterium acnes ) has been antimicrobial activity, and through the actual clinical experiments confirmed that the skin whitening activity, skin moisturizing activity, acne improvement activity, atopic dermatitis improvement activity.

The present invention is provided based on these experimental results, and in one aspect relates to a skin external preparation composition for skin whitening comprising bamboo shoot powder or bamboo shoot extract as an active ingredient, and in another aspect, the bamboo shoot powder or bamboo shoot extract is an active ingredient. The present invention relates to a skin external preparation composition for moisturizing skin, and in another aspect, to an external preparation composition for improving acne comprising bamboo shoot powder or bamboo shoot extract as an active ingredient, and in another aspect, a bamboo shoot powder or bamboo shoot extract. It relates to a topical skin composition for atopic dermatitis improvement comprising as an active ingredient.

As used herein, the term "active ingredient" alone refers to a component that can exhibit the desired activity or exhibit itself with a carrier which is inactive.

In the present specification, the term "bamboo shoot extract" is obtained by extracting bamboo shoots to be extracted regardless of the extraction method with methanol, distilled water, ethanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or a mixed solvent thereof. It is understood as meaning including the crude extract and the extract obtained by fractionating the crude extract with the solvents listed above. Regardless of the extraction method, as long as the extraction object is extracted through the step of immersing in the extraction solvent, the extraction method may be any method such as cooling, refluxing, heating, ultrasonic radiation, and the like. Nevertheless, the "bamboo shoot extract" preferably refers to those obtained by extracting the extraction object with water, distilled water, ethanol or a mixed solvent thereof. Such extracts or fractions may be liquid concentrates or solids from which the extraction or fraction solvents have been removed.

Terms not specifically defined herein follow the Korean dictionary meaning or the meaning generally accepted in the art.

Meanwhile, the composition of the present invention (that is, the skin external preparation composition for skin whitening, the skin external preparation composition for skin moisturizing, the external preparation composition for improving acne and the external preparation composition for improving atopic dermatitis; the same as below) is the bamboo shoot powder or extract as an active ingredient thereof. May be included in any amount (effective amount) according to the use, formulation, formulation purpose, etc., as long as it can exhibit skin lightening activity, skin moisturizing activity, acne improving activity, atopic dermatitis improving activity, and the conventional effective amount is It will be determined in the range of 0.001% to 20.000% by weight based on weight. Here, the "effective amount" refers to the amount of active ingredients that can promote skin whitening activity, skin moisturizing activity, acne improving activity, atopic dermatitis improving activity. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

The composition of this invention can be grasped | ascertained as a cosmetic composition in a specific aspect.

When the composition of the present invention is conceived as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and Aqueous), anhydrous products (oil and glycol based), gels, masks, packs, powders and the like.

The composition of the present invention may include an acceptable carrier in a cosmetic preparation in addition to including the active ingredient.

Herein, "acceptable carrier in cosmetic preparation" means a compound or composition already known and used that can be included in cosmetic preparations or a compound or composition which will be developed in the future and does not have more toxicity than the body can adapt to when contacted with the skin.

The carrier may be included in the composition of the present invention from about 1% to about 99.99% by weight, preferably from about 50% to about 99% by weight of the composition.

However, it is understood that the ratios limit the scope of the present invention in any aspect because the ratios vary depending on the formulation of the cosmetics as described above and their specific application site (face or hand) or their preferred application amount. It should not be.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

The composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by including an active ingredient thereof in a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a hard water softener, and the like as an additive. .

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti or the like may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion sequestrant, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally added in an amount of 99.999 wt% to 50 wt% based on the total weight of the soap composition. The additive may be added in an amount of 1% to 20% by weight.

The composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier, excipient, and the like in addition to the active ingredient, and may be a topical formulation such as creams, lotions, ointments (semi-solid external preparations), micro-emulsions, gels, pastes, transdermal preparations. (TTS) (such as patches, bandages, etc.) and the like.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) is not as toxic as applicable, without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, Solid lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, emulsifiers (such as TWEENS), wetting agents (Such as sodium lauryl sulfate), colorants, flavors, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions, and the like. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, suspending agents and dispersants such as sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethyl cellulose, sodium alginate, polyvinylpyrrolidone, etc. Can be.

The pharmaceutical composition of the present invention may be administered orally or parenterally, preferably topically.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

As described above, according to the present invention, it is possible to provide a skin whitening composition for skin whitening using bamboo shoots, a skin external preparation composition for skin moisturizing, a skin external preparation composition for improving acne, and an external skin composition for improving atopic dermatitis.

Such a composition of the present invention may be commercialized as a drug such as cosmetics or soap, ointment, cream.

Hereinafter, the present invention will be described with reference to Examples, Comparative Examples, and Experimental Examples. However, the scope of the present invention is not limited to these examples, comparative examples and experimental examples.

< Example > Preparation of Bamboo Shoot Powder or Extract

Example 1 Preparation of Bamboo Shoot Powder

Bamboo shoots were dried and pulverized to prepare bamboo shoot powder (particle size).

Example 2 Preparation of Bamboo Shoot Extract

1 kg of crushed bamboo shoots were immersed in 70% ethanol and extracted with stirring for 24 hours. The extract was filtered through Whatman No. 2 filter paper and concentrated under reduced pressure to obtain a powdery bamboo shoot extract.

< Experimental Example > Skin whitening activity experiment, skin moisturizing activity experiment, acne improvement activity experiment and atopic dermatitis improvement activity experiment

Experimental Example 1 Whitening Activity Experiment

Experimental Example 1-1 Whitening Active Cell Experiment-Melanin Production Inhibitory Activity Experiment

After dispensing B16F10 cells (2.0 × 10 ⁴ cells / ml) into 24 wells, the bamboo shoot powder or extract (20 μg / ml) of the above example was treated with α-MSH (50 nM), a melanogenesis-stimulating agent, for 72 hours. Incubated for Cells were harvested and 1N NaOH was added to completely lyse the cells, and then absorbance was measured by ELISA at 405 nm.

The results are shown in Table 1 below as a percentage of the control group (α-MSH treated group).

Melanin production inhibition rate (%) division Melanin production inhibition rate (%) Control 100 Bamboo Shoot Powder of Examples 79.3 ± 2.82 * <Example 2> of the bamboo shoot extracts 63.4 ± 3.61 * * p <0.05,

Looking at the results of [Table 1], it can be seen that the bamboo shoot powder or extract of Example both have melanin production inhibitory activity.

Meanwhile, although not shown in the experimental results, the bamboo shoot powder or extract of the above example did not show any particular cytotoxicity.

Experimental Example 1-2 Whitening Effect Sensory Evaluation Experiment

Cosmetic composition containing bamboo shoot powder or extract for the sensory evaluation experiment of the whitening effect (3.0% by weight of bamboo shoot powder or extract of Example, 7.0% by weight of ethanol, 3.0% by weight of glycerin, 3.0% by weight of 1,3-butylglycol, polysol Bait20 0.2% by weight, polyoxyethylene alkyl ether 0.5% by weight, sodium hyaluronate 3.0% by weight, fragrance trace amount, preservative trace amount, the remaining amount of purified water; the cosmetic composition containing bamboo shoot powder below is the cosmetic composition of <Production Example 1> As a composition, the cosmetic composition containing the bamboo shoot extract is divided into the cosmetic composition of <Production Example 2>).

As a cosmetic composition of the comparative example, instead of the extract of the example, a cosmetic composition containing the amount of purified water was prepared and used.

Sensory evaluation experiment on the whitening effect of 30 women in their 20s and 30s as a group of experiments were used once or twice a day for 8 weeks in accordance with the usual method of use and then the 5-point scale method (very good (5 points) ), Excellent (4 points), moderate (3 points), poor (2 points), very bad (1 point).

 The results are shown as average values in [Table 2] below.

Whitening effect sensory evaluation results division result Preparation Example 1 3.92 Preparation Example 2 4.42 Comparative Example (No Bamboo Shoot Powder or Extract) 3.16

Through the sensory evaluation results of the [Table 2] it can be seen that the bamboo shoot powder or extract of the embodiment has a whitening activity.

Experimental Example 2 Clinical Experiment on Moisturizing Effect

The clinical experiment on the moisturizing effect is to use 30 women in their 20s and 30s as an experimental group of each group, the cosmetic composition prepared in <Experimental Example 1-1> once or twice a day for each group According to 8 weeks.

After 8 weeks, skin moisture loss (TEWL) (Tewameter TM300, C + K electronic GmbH. Germany) and skin moisture content (Corneometer CM825, C + K electronic GmbH. Germany) were measured and the results averaged. The standard deviations are shown in Tables 3 and 4 below.

In the above, TEWL is shown to be high as the skin is dry, and the skin moisture content is shown as low as the skin is dry.

TEWL division 0 parking Week 8 Example 1 18.92 ± 5.54 15.53 ± 3.63 * Example 2 18.27 ± 6.43 14.93 ± 4.76 * Comparative example 17.68 ± 5.48 18.17 ± 6.93 * p <0.05

Skin moisture content division 0 parking Week 8 Preparation Example 1 37.7 ± 4.83 39.2 ± 6.52 Production Example 3 37.4 ± 5.67 41.2 ± 5.91 * Comparative example 37.4 ± 6.54 37.5 ± 5.29 * p <0.05

Looking at the results of [Table 3] and [Table 4], when using the cosmetic composition containing bamboo shoot powder or extract of the Example showed a tendency to decrease TEWL and increase the skin moisture content. However, there was no statistical difference in the case of the lotion composition containing bamboo shoot powder.

Experimental Example 3 Experiments on Acne Improvement Activity

Experimental Example 3-1 Antimicrobial Activity Test against Acne Bacteria

Propionibacterium acnes , an acne bacterium, was incubated at 37 ° C. for 72 hours in a medium to which bamboo shoot powder or extract of Example was added at a constant concentration (0.001 to 0.5% (w / v)), and the growth of the acne bacterium The medium addition concentration of the bamboo shoot powder or extract of this Example of inhibition is shown in [Table 5] as Minimum Inhibitiry Concentration (hereinafter referred to as "MIC").

As the positive control group was used benzoyl peroxide known as acne treatment.

Minimum Development Concentration (MIC) division MIC Preparation Example 1 0.06 Production Example 2 0.03 Positive control group 0.02

The results of Table 5 show that bamboo shoot powder or extract of Example showed antibacterial activity against acne bacteria at 0.06% and 0.03%, respectively. The positive control group benzoyl peroxide showed antimicrobial activity at 0.02%.

<Experimental Example 3-2> Sensory evaluation experiment on the effect of improving acne

Sensory evaluation experiment for the acne improvement effect of the bamboo shoot powder or extract of the above embodiment was also performed using the cosmetic composition prepared in the <Experimental Example 2-1>.

Thirty females with acne symptoms among the women in their late teens to twenties ages were used in the experimental group of one group for facial treatment once or twice a day for eight weeks according to a normal use method for eight weeks.

The results are shown in [Table 6] below.

division Preparation Example 1 Production Example 2 Comparative example 4 Weeks + +++ ± 8 Weeks ++ +++ ± +: Slight improvement
++: good improvement
+++: Very good improvement
±: not improved nor deteriorated
_: Worse

The results in Table 6 show that the cosmetic composition containing bamboo shoot powder or extract has acne improving activity.

Experimental Example 3 Clinical Study of Atopic Dermatitis Improvement Activity

Five children suffering from atopic dermatitis were applied to the cosmetic composition prepared in <Experimental Example 2-1> as a test group of one group, twice a morning and evening for six weeks.

The atopy improvement effect after 6 weeks was evaluated by SCORAD index, and it is shown in following [Table 7].

SCORAD Index division Before application (average value) After application (average value) Preparation Example 1 68.43 46.93 Production Example 2 69.37 36.82 Comparative example 67.29 68.82

The results of Table 7 show that the bamboo shoot powder or extract of the above example has atopy improving activity.

Claims (8)

Skin external preparation composition for skin whitening comprising bamboo shoot powder or bamboo shoot extract as an active ingredient.
Skin external preparation composition for moisturizing skin containing bamboo shoot powder or bamboo shoot extract as an active ingredient.
Skin external preparation composition for acne improvement comprising bamboo shoot powder or bamboo shoot extract as an active ingredient.
Skin topical composition for improving atopic dermatitis comprising bamboo shoot powder or bamboo shoot extract as an active ingredient.
5. The method according to any one of claims 1 to 4,
The bamboo shoot extract is an external composition for skin, characterized in that obtained by dipping bamboo shoots with a solvent mixture of water and ethanol.
5. The method according to any one of claims 1 to 4,
The composition for external application for skin, characterized in that the cosmetic composition.
5. The method according to any one of claims 1 to 4,
The composition for external application for skin, characterized in that the soap composition.
5. The method according to any one of claims 1 to 4,
The composition for external application of skin, characterized in that the pharmaceutical composition.