KR101186925B1 - Skin Whitening Composition Using a Mulberry Extract - Google Patents

Abstract

The present invention discloses a skin lightening composition using an audi extract and a yulmu seed extract having tyrosinase inhibitory activity and melanin production inhibitory activity.

Audi, extract, pearl seed, whitening

Description

Skin Whitening Composition Using a Mulberry Extract

The present invention relates to a skin lightening composition using an Audi extract and Yulmu seed extract.

It is well known that skin whitening is directly related to melanin pigment.

Melanin protects sub-dermal skin organs by blocking UV rays, and protects the damage of proteins and genes in the skin caused by free radicals in the skin. It causes hyperpigmentation such as blemishes and freckles.

Melanin synthesis is achieved by the action of tyrosinase and tyrosinase related proteins present in the melanosomes. Specifically, tyrosine is oxidized by tyrosinase and is converted into dopa (DOPA, 3,4-dihydroxyphenylalanine), followed by oxidization of dopa oxidase (dopaoxidase) to dopaquinone. In addition, melanin is produced by the action of tyrosinase-related proteins TRP-1 (5,6-dihydroxy indole-2-carboxylic acid oxidase) and TRP-2 (dopachrome tautomerase).

Therefore, when the activity of tyrosinase is inhibited or the production of melanin is inhibited, a whitening effect can be expected.

Korean Patent Publication No. 2008-0105808 (the active ingredient is a phenolic compound isolated from the root), and Korean Patent No. 090535 (the active ingredient is a phenolic compound isolated from the wood vinegar) as a whitening effect and tyrosinase inhibitory activity And melanin production inhibitory activity is disclosed.

An object of the present invention relates to a skin whitening composition using an audi extract and a yulmu seed extract having a tyrosinase inhibitory activity and a melanin production inhibitory activity.

As the present invention is confirmed in the following Examples and Experimental Example, when the hydrothermal extract of the Audi hydrothermal extract and Yulmu seeds were treated to B16F10 melanoma cells, all of these extracts showed tyrosinase inhibitory activity and melanin production inhibitory activity. In addition, when these extracts were treated with B16F10 melanoma cells together, they were completed by confirming that tyrosinase inhibitory activity and melanin production inhibitory activity were remarkably high.

Therefore, the skin whitening agent composition of the present invention is characterized in that it comprises an Audi extract and Yulmu seed extract as an active ingredient.

In the present specification, "audi" means a mulberry fruit also known as loss, and according to the synonym of Bogam or Hwahan Encyclopaedia, it is known to be beneficial to diabetes and five intestines and to treat swelling and black hair.

In addition, in the present specification, "extract" refers to an extract obtained by extracting an extraction object with ethanol, methanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water or a mixed solvent thereof regardless of the extraction method and the extract thereof. It is understood as meaning including extracts fractionated with the solvents listed above. Regardless of the extraction method, as long as the extraction object is extracted through the step of immersing in the extraction solvent, it should be understood that the extraction method can be applied to any method such as cooling, refluxing, heating, and ultrasonic wave. Nevertheless, the extract is preferably obtained by extracting the object to be extracted with water, ethanol or a mixed solvent thereof, and is meant to include a concentrated liquid extract or solid extract from which the extraction solvent is removed.

In addition, in the present specification, "skin whitening" is understood as a result of the inhibition of the production of melanin, specifically, means the prevention, delayed expression or treatment of symptoms caused by the production of melanin, such as blemishes, freckles, skin aging, etc. .

In addition, in the present specification, "active ingredient" means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

Terms not specifically defined herein follow the Korean dictionary meaning or meaning commonly used in the art.

The skin whitening agent composition of the present invention may be used in any amount (effective amount) according to the use, formulation (cosmetic, soap, ointment, etc.), blending purpose, etc., as long as it can exhibit skin whitening activity of the extracts of the active extracts and the extract of Yulmu as its active ingredients. A typical effective amount will be determined within the range of 0.001% to 99.900% by weight based on the total weight of the composition. Here, the "effective amount" refers to the amount of the active ingredient that can induce a skin lightening effect. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

The skin lightening composition of the present invention may include other well-known compounds or plant extracts that are known to have a skin whitening effect so as to enhance or enhance the skin whitening effect, in addition to the extracts of the active ingredient and the extract of Yulmu seed, which are the active ingredients. In addition, it may include a compound or plant extract having a skin lightening effect to be revealed in the future.

Herein, the other compounds or plant extracts include mercaptosuccinic acid, mercaptodextran, tefrenone, dihydroxy-isoquinoline, indomethacin, 3-hydroxymanul, vitamin K, thiazolidone, kynurenine, and mulberry leaves. Extracts, mulberry stem extracts, mulberry root extracts, lemon extracts, cucumber extracts, licorice extracts, rosemary extracts, acerola cherry extracts, ginkgo extracts, carob extracts, geranium extracts, herbal extracts, etc. However, the present invention is not limited thereto.

Compounds or plant extracts as exemplified above may be included in the skin whitening agent composition of the present invention together with one or more of the active extracts and the mulberry seed extract.

In another aspect, the present invention relates to a composition for alleviating skin irritation.

The composition for alleviating skin irritation of the present invention is characterized in that it comprises an Audi extract and / or Yulmu seed extract as an active ingredient.

The following experimental example shows that skin irritation is alleviated when using a cosmetic pack containing an Audi extract and / or a yulmu seed extract compared to a cosmetic pack containing no extract.

In particular, when both the Audi extract and Yulmu seed extract are included, the skin irritation effect is high. Therefore, it is preferable that all of these extracts are included in the composition for alleviating skin irritation of the present invention.

 As used herein, "skin irritation relief" means skin itching, skin itching and / or erythema is suppressed or improved.

The skin whitening agent composition of the present invention and the composition for alleviating skin irritation (hereinafter "composition of the present invention") can be regarded as a cosmetic composition in a specific embodiment.

When the composition of the present invention is conceived as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and Aqueous), anhydrous products (oil and glycol based), gels, masks, packs or powders and the like.

The composition of the present invention may comprise an acceptable carrier in cosmetic preparations in addition to the Audi extract and the yulmu seed extract.

Herein, "acceptable carriers in cosmetic preparations" are compounds or compositions already known and used that may be included in cosmetic preparations, or compounds or compositions which will be developed in the future, which have no toxicity, instability or irritation more than the body can adapt to in contact with the skin. Say that.

The carrier may be included in the composition of the present invention from about 1% to about 99.99% by weight, preferably from about 50% to about 99% by weight of the composition.

However, since the ratio depends on the form of the above-described preparation of the cosmetic of the present invention and its specific application site (face or hand) or its preferred application amount, the ratio is in any aspect the scope of the present invention. It should not be understood as limiting.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, humectants, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents and perfumes.

The components of the alcohol, oil, surfactant, fatty acid, silicone oil, wetting agent, moisturizer, viscosifier, emulsion, stabilizer, sunscreen agent, coloring agent and fragrance which can be used as the carrier are already well known in the art, The substance / composition can be selected and used.

The composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by including an AUDI extract and Yulmu seed extract which are active ingredients on a soap base, and as an additive, a skin moisturizer, an emulsifier, a water softener, and the like. It can be prepared to include.

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti or the like may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion blocking agent, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally 99.9 to 50 wt% based on the total content of the soap composition. It may be added, the additive may be added in 1% to 20% by weight.

Meanwhile, the composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

When the composition of the present invention is identified as a pharmaceutical composition, the pharmacological effect may be understood as a therapeutic or prophylactic effect for a disease resulting from an increase in melanin, and the skin whitening is understood as a result of the treatment or prevention of the disease. Can be. As a disease resulting from the increase of the melanin means blemishes, freckles, skin aging and the like.

The pharmaceutical composition of the present invention includes oral formulations (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral forms, including pharmaceutically acceptable carriers, excipients, etc., in addition to the active extracts and extracts of the seed extract. Formulations (sterile injectable aqueous or oily suspensions), topical formulations (solutions, creams, ointments, gels, lotions, patches) and the like.

As used herein, "pharmaceutically acceptable" means not inhibiting the activity of the active ingredient, but having no toxicity above that the subject of application (prescription) is adaptable.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, solids Lubricants (e.g. stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (e.g. peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g. propylene glycol, glycerin, etc.), alginic acid, emulsifiers (e.g. TWEENS), wetting agents (e.g. Sodium lauryl sulfate), colorants, flavoring agents, tableting agents, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions and the like. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, when the pharmaceutical composition of the present invention is prepared with an aqueous suspending agent, suitable excipients are sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethylcellulose And suspending agents and dispersing agents such as sodium alginate and polyvinylpyrrolidone. Suitable excipients when prepared from injection solutions include Ringer's solution, isotonic sodium chloride, and the like.

The pharmaceutical compositions of the present invention may be administered orally or parenterally, but will usually be administered topically to the skin, usually in a topical formulation.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

As described above, according to the present invention, it is possible to provide a skin lightening composition using an Audi extract. In addition, the present invention can provide a composition for alleviating skin irritation. The composition of the present invention can be formulated into a drug such as cosmetics, soap or ointment.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Preparation of Audi Extract and Yulmu Seed Extract

Example 1 Preparation of Audi Extract

1 g of water was added to 50 g of Audi, and heated to a temperature of about 65 ° C. and extracted for 12 hours. After extraction, the resultant was filtered and the residue was extracted.

Example 2 Preparation of Yulmu Seed Extract

1L of water was added to 50 g of Yulmu seed powder, and heated to a temperature of about 65 ° C. and extracted for 12 hours. After extraction, the resultant was filtered and the residue was extracted and concentrated in dark and dark to obtain a solid Yulmu seed extract.

< Experimental Example > Whitening effect, atopic dermatitis improvement effect and moisturizing effect experiment

Experimental Example 1 Whitening Effect Cell Experiment

Experimental Example 1-1 B16F10 Melanoma Cell Culture

B16F10 melanoma cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS), 100 units / ml penicillin, and 100 units / ml streptomycin at 37 ° C. and maintained at 5% CO ₂ . Subculture was performed once.

Experimental Example 1-2 Cytotoxicity Evaluation

Toxicity to the B16F10 cells of the Example extract was evaluated using the MTT assay. B16F10 cells (2.0 × 10 ⁴ cells / ml) were dispensed into 24 wells and cultured for 18 hours, followed by further incubation for 24 hours by treating a sample (10 μg / ml) (the extract of each example above). 200 µl of the MTT solution prepared at a concentration of 5 mg / ml PBS was added thereto, followed by further 4 hours of incubation. The culture solution was removed, and 200 µl of DMSO was added to each well, and the absorbance of the formazan produced by MTT reduction was measured at 570 nm.

The results are shown in FIG. 1. Referring to Figure 1, it can be seen that the extracts of the above examples do not show any particular cytotoxicity (A: Yulmu seed extract, B: Audi extract).

Experimental Example 1-3 Tyrosinase Inhibitory Activity Experiment

After dispensing B16F10 cells (2.0 × 10 ⁴ cells / ml) into 24 wells, the sample (10 μg / ml) (the same amount of the extract of Example 1 or the extract of Example 1 and the extract of Example 2) Mixture) and incubated for 48 hours. Next, the cells of each well were collected, suspended in 100 µl of 10 mM PBS, the suspension was vortexed, and the supernatant obtained by centrifugation was used as an enzyme solution. Tyrosinase inhibitory activity experiment was carried out in 24 wells of the supernatant 40μL and its substrate L-DOPA (2mg / ㎖) 100μL, reacted for 1 hour at 37 ℃ and absorbance at 405nm using an ELISA reader Was measured. The activity of tyrosinase is shown in FIG. 2 as a percentage of the absorbance of the untreated group control.

Referring to Figure 2, it can be seen that the extracts of the above examples all inhibit the activity of tyrosinase. In particular, the activity of the mixture of the Audi extract and Yulmu seed extract is the highest (A: Yulmu seed extract, B: Audi extract).

Experimental Example 1-4 Melanin Production Inhibition Activity Experiment

After dispensing B16F10 cells (2.0 × 10 ⁴ cells / ml) into 24 wells, each extract (10 μg / ml) of the above Example was treated with α-MSH (50 nM), a melanin-forming stimulant, and cultured for 72 hours. It was. Cells were harvested and 1N NaOH was added to completely lyse the cells, and then absorbance was measured by ELISA at 405 nm.

The results are shown in FIG. 3 as a percentage of the control group (α-MSH treated group). Referring to FIG. 3, it can be seen that the extract of Example inhibits the production of melanin. Here, similar to the result of FIG. 2, the mixture of the Audi extract and the yulmu seed extract have the highest melanin production inhibitory activity. (A: Yulmu seed extract, B: Audi extract).

Experimental Example 2 Whitening Effect Clinical Experiment

For the clinical experiment of the whitening effect was prepared a cosmetic pack with the ingredients and contents as shown in Table 1 below.

[Table 1]

Ingredients and content of cosmetic pack (% by weight)

Hangul Name Production Example 1 Production Example 2 Purified water TO 100 Carbomer 0.2 Butylene Glycol 3.0 Sodium hyaluronate 0.2 Methylparaben 0.2 Bis-fig-18 methethyldimethylmethylsilane 0.5 Cetyl alcohol 0.5 Stearyl alcohol 3.0 Glyceryl stearate 0.5 Ceteares-25 0.5 Shea Butter 0.2 Example of an Audi Extract 3.0 3.0 Yulmu Seed Extract of Examples 3.0 - Spices 0.2 Anthocyanin 0.05 Ocher powder 20 Triethanolamine 0.2

Here, as a cosmetic pack of Comparative Example, a cosmetic pack containing purified water was used instead of the extract of Example.

For the whitening effect test, 20 women in their 20s and 20 women in their 30s were used twice a week for 4 weeks according to the usual method of use, and then a 5-point scale method (very good (5 points), Excellent (3 points), moderate (0 points), bad (-3 points), very bad (-5 points) was evaluated according to the results are shown in Tables 2 to 5 below. In the case of skin irritation, which is an evaluation item, it was examined whether the phenomenon of itching, skin itching or erythema of the skin was alleviated.

[Table 2]

Results of the whitening effect of <Production Example 1> (Women in 20s)

number Skin irritation Whitening effect Production Example 1 Comparative example Manufacturing example Comparative example One 3 3 3 3 2 5 3 5 3 3 5 5 5 5 4 5 5 5 5 5 5 5 3 3 6 5 3 5 3 7 3 3 5 3 8 3 3 3 0 9 5 0 5 3 10 5 3 5 5 11 3 5 5 5 12 5 0 3 0 13 5 5 5 3 14 5 3 5 3 15 5 5 3 0 16 5 3 5 3 17 5 5 3 3 18 3 5 5 3 19 5 0 5 5 20 5 3 3 3 Average 4.50 3.35 4.30 3.05

[Table 3]

Results of the whitening effect of <Production Example 1> (women in their 30s)

number Skin irritation Whitening effect Production Example 1 Comparative example Production Example 1 Comparative example One 5 5 5 0 2 3 0 3 3 3 3 3 5 3 4 3 3 5 0 5 5 5 3 3 6 3 3 5 3 7 5 0 5 3 8 5 5 3 3 9 3 3 3 3 10 5 5 3 3 11 3 3 5 5 12 3 5 3 3 13 5 5 5 3 14 5 3 5 0 15 3 3 3 0 16 5 3 5 3 17 5 5 5 3 18 3 3 5 5 19 0 0 5 5 20 5 3 3 3 Average 3.85 3.25 4.20 2.70

[Table 4]

Results of the whitening effect of <Production Example 2> (women in their 20s)

number Skin irritation Whitening effect Production Example 2 Comparative example Production Example 2 Comparative example One 3 3 3 3 2 3 3 5 3 3 3 3 3 5 4 5 5 3 3 5 3 3 5 3 6 3 3 3 0 7 5 3 3 3 8 3 3 3 0 9 3 5 5 3 10 5 3 3 5 11 5 0 3 3 12 3 0 3 3 13 3 3 3 3 14 3 3 5 3 15 5 3 5 3 16 5 5 3 3 17 3 3 0 0 18 3 3 5 0 19 3 3 3 3 20 3 0 3 3 Average 3.60 2.85 3.45 2.60

[Table 5]

Results of the whitening effect of <Production Example 2> (women in their 30s)

number Skin irritation Whitening effect Production Example 2 Comparative example Production Example 2 Comparative example One 3 3 3 3 2 3 3 3 3 3 5 5 5 3 4 3 3 3 0 5 5 3 3 0 6 3 3 3 3 7 5 0 5 5 8 3 3 3 3 9 3 0 5 3 10 5 3 5 3 11 3 5 3 3 12 3 3 3 0 13 3 3 5 3 14 3 3 3 5 15 3 0 3 3 16 3 3 3 3 17 3 3 0 0 18 3 3 3 0 19 3 3 3 3 20 3 3 3 3 Average 3.40 2.75 3.35 2.45

Referring to the above [Table 2] to [Table 5], it can be seen that the extract of the embodiment does not cause skin irritation and of course, has an effect of alleviating skin irritation. In addition, it can be seen that the whitening effect is also superior to the comparative example. In particular, in the case of a cosmetic pack containing both the Audi extract and Yulmu seed extract, it can be seen that the whitening effect and the skin irritation-reducing effect are remarkably superior to the cosmetic pack containing only the Audi extract.

1 is a result showing that the Audi extract and Yulmu seed extract does not have cytotoxicity. Data is shown as mean ± standard deviation of three experiments.

Figure 2 shows the results of the tyrosinase inhibitory activity of the Audi extract and / or Yulmu seed extract. Data is shown as mean ± standard deviation of three experiments.

Figure 3 is a result showing the melanin production inhibitory activity of the mulberry extract and / or yulmu seed extract. Data is shown as mean ± standard deviation of three experiments.

Claims (12)

Skin whitening cosmetic composition comprising the same amount of a mixture of water extract of Audi and water extract of Yulmu seed as an active ingredient. delete delete The method of claim 1, The composition is a skin whitening cosmetic composition, characterized in that it further comprises one or more of mulberry leaf extract, mulberry stem extract, mulberry root extract, 칡 extract. delete Skin whitening soap composition comprising the same amount of a mixture of water extract of Audi and water extract of Yulmu seed as an active ingredient. delete delete delete delete delete delete

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