KR20080096162A - Cosmetic composition for whitening skin - Google Patents

Abstract

A composition for skin whitening is provided to improve skin aging, chloasma or fleck and to be used as cosmetic, food, drugs and soap composition. A composition for skin whitening includes an extract selected from the group consisting of ginseng extract and bamboo extract as an active ingredient. The skin whitening is generated by hindering melanogenesis. The ginseng extract is obtained by extracting ginseng in ethanol, water or a mixed solvent thereof. The bamboo extract is obtained by extracting the bamboo in ethanol, water or a mixed solvent thereof.

Description

Cosmetic composition for whitening skin {Cosmetic Composition for Whitening Skin}

The present invention relates to a composition for skin whitening, and more particularly to a composition for skin whitening comprising ginseng extract and / or bamboo extract having a melanin production inhibitory effect.

It is well known that skin whitening is directly related to melanin pigment. Melanin is made by tyrosine (tyrosine) is oxidized by tyrosinase (tyrosine) to dopa (DOPA, 3,4-dihydroxyphenylalanine), dopa is oxidized back to dopaquinone (dopaquinone), and then made through a non-enzymatic oxidation reaction.

Melanin protects sub-dermal skin organs by blocking UV rays, and protects the damage of proteins and genes in the skin caused by free radicals generated in the skin. It causes hyperpigmentation such as blemishes and freckles.

Therefore, inhibiting the production of melanin can bring the effect of skin whitening.

Inhibiting melanin production has the effect of skin whitening, para- methoxy phenol ( p- metoxyphenol), hydroquinone (hydroquinone), kojic acid (kojic acid), arbutin (albutin), vitamin C (ascorbic acid), Compounds such as glutathione and cysteine, and plant extracts such as lettuce extract, licorice extract, peony extract, cinnamon extract, brown root extract, Angelica extract, bark extract, halved extract, aloe extract and the like are known.

The present invention is also conceived in that ginseng extract and the like inhibit the synthesis of melanin.

An object of the present invention to provide a composition for skin whitening.

Other objects, specific embodiments, and the like of the present invention are presented below.

In one aspect, the present invention relates to a composition for skin whitening.

The present inventors, as confirmed in the following Examples and Experimental Examples, having the extract of ginseng and bamboo extracted with 70% ethanol and a mixture of the extract, the inhibitory effect of tyrosinase activity known to synthesize melanin from tyrosine and As a result of experiments on the effect of inhibiting the synthesis of melanin, which causes blemishes, etc., not only each extract has an effect of inhibiting tyrosinase activity and melanin synthesis, but also a mixture of each extract, in particular, tyrosinase activity and melanin synthesis. It was confirmed that the effect of suppressing the high.

The present invention is provided based on the above experimental results.

Therefore, in one aspect, the composition having a skin whitening effect of the present invention (hereinafter "skin whitening composition") is a ginseng extract and / or bamboo extract having an inhibitory effect of tyrosinase activity and / or inhibitory effect of melanin synthesis It is characterized by including.

In the present specification, including the claims, "skin whitening" is understood as the resulting phenomenon as the synthesis of melanin is inhibited.

This is because the ginseng extract and / or the bamboo extract included in the composition for skin whitening of the present invention as an active ingredient, as shown in the following Examples and Experimental Examples, has a synthetic inhibitory activity of melanin.

As a result of the suppression of the synthesis of melanin, the result is specifically a symptom resulting from the increase of melanin as the synthesis of melanin is inhibited (when the composition for skin whitening of the present invention is identified as a pharmaceutical composition, Disease can be identified or alleviated.

Since melanin is known to cause skin aging if it causes more than necessary and causes hyperpigmentation such as blemishes and freckles, the symptoms resulting from the increase of melanin may include blemishes, freckles, and skin aging. As shown in the following Examples and Experimental Examples of the present invention, as long as it is obvious that ginseng extract and / or bamboo extract included in the skin whitening composition of the present invention inhibits the synthesis of melanin, the above symptom is a symptom resulting from an increase in melanin. It should be understood that it includes all the symptoms leading to skin whitening as a result of improving or alleviating it.

Therefore, such a symptom includes not only the symptoms which have been revealed to the present but also the symptoms which will be revealed in the future, but is preferably understood to mean symptoms that are known so far, more preferably the symptoms as exemplified above, ie blemishes, freckles, skin aging It is understood to mean.

Also in the above and below, including the claims, the term "extract" refers to any subject to extract, ie ginseng or bamboo, from any extraction solvent such as methanol, distilled water, ethanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or It refers to what is obtained by extraction with these mixed solvents, Preferably it refers to what was obtained using alcohol, especially ethanol, water, or these mixed solvents as an extraction solvent.

"Ginseng" as defined above and below including claims, is defined as including all ginseng regardless of processing method, cultivation method and cultivation area. Ginseng is peeled from fresh ginseng immediately after harvest and white ginseng obtained by natural drying or peeling artificially (for example, heating, humidification, hot air drying, etc.) after peeling from fresh ginseng, depending on processing method. It is divided into red ginseng obtained by steaming and drying without peeling off. According to the cultivation method, it is divided into cultivated ginseng obtained by artificial cultivation in ginseng field, camphor ginseng obtained by spraying ginseng seed in the mountain, and wild ginseng grown in the mountain. Depending on the cultivation region, Korean ginseng produced in the Korean peninsula, Hwagi ginseng produced in North America, such as the United States and Canada, Jeonchisam produced in China, and bamboo rice ginseng produced in Japan are classified into the composition for skin whitening of the present invention. As ginseng extract included, extracts obtained from all these kinds of ginseng are used. It can be (including wild ginseng or ginseng recently the muscle of ginseng roots of a new form of induction by plant biotechnology wild ginseng adventitious root and adventitious roots).

It is also to be understood that the term "bamboo" of the above and below, including the claims, of any kind. Thus, regardless of whether it is bamboo belonging to Phyllostachys, bamboo belonging to Arundinaria, bamboo belonging to Sasa or bamboo belonging to Pseudosasa, If the plant (ie rice paddy family), all can be used in the method for producing the bamboo extract of the present invention.

On the other hand, when producing a ginseng extract or bamboo extract, the ginseng or bamboo extract to be extracted may be used to be chopped to a suitable size, or pulverized to a particle size of 1 to 2cm or pulverized to a particle size of 1cm or less.

As long as it is based on the following Examples and Experimental Examples, it is obvious that even if ginseng or bamboo extract to be extracted is used in any size at the time of extraction, the extracts will have a skin whitening effect.

The composition for skin whitening of the present invention may include, in addition to ginseng extract and / or bamboo extract, a compound or plant extract already known to have a skin whitening effect so as to enhance or enhance the skin whitening effect, such a compound or plant Extracts include para-methoxyphenol ( p- metoxyphenol), hydroquinone, kojic acid, arbutin, vitamin C (ascorbic acid), glutathione, cysteine, etc. Compounds, Morus Extract, Licorice Extract, Peony Extract, Cinnamon Extract, Root Extract, Angelica Extract, Barberry Extract, Half Extract, Aloe Extract, Mercaptosuccinic Acid, Mercaptodextran, Tefrenone, Dihydroxy-isoquinoline, India Methacin, 3-hydroxymanul, vitamin K, thiazolidone, kynurenine, lemon extract, cucumber extract, mulberry extract, licorice extract, rosemary chu Include water, acerola cherry extract, ginkgo extract, karop (carob) extract, geranium (geranium) extract, herbal extracts, such as, but not limited thereto.

The compound or plant extract as exemplified above may be included in the composition for skin whitening of the present invention together with ginseng extract and / or bamboo extract.

On the other hand, in the composition for skin whitening of the present invention, ginseng extract and / or bamboo extract may be included in any amount within a range that does not have toxicity to the human body depending on the degree of skin whitening required, specifically the composition 0.05 wt% to 99.99 wt% with respect to the total weight.

On the other hand, in the composition for skin whitening of the present invention, both ginseng extract and bamboo extract are preferably included. It is because the skin whitening effect is higher when both ginseng extract and bamboo extract are included as shown in the following experimental example.

In another aspect, the skin whitening composition of the present invention is characterized by comprising ginseng powder and / or bamboo powder.

In the following Examples and Experimental Examples of the present invention was confirmed that the ginseng extract and bamboo extract has a tyrosinase activity inhibitory effect and melanin synthesis inhibitory effect, ginseng powder and bamboo powder also inhibits tyrosinase activity and melanin synthesis It is obvious that it will have an effect.

In the composition for skin whitening of the present invention comprising ginseng powder and / or bamboo powder, the content of ginseng powder and / or bamboo powder as an active ingredient, its preferred embodiment and the like include ginseng extract and / or bamboo extract As described above in relation to the skin whitening composition is effective as it is.

The skin whitening composition of the present invention can be regarded as a cosmetic composition in a specific embodiment.

When the composition for skin whitening of the present invention is understood as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsion, lotion, cream (oil-in-water type, water-in-oil type, multiphase), solution, suspension (Anhydrous and aqueous), anhydrous compounds (oil and glycols), gels, masks, packs or powders, and the like.

The cosmetic composition for skin whitening of the present invention may include an acceptable carrier in cosmetic preparations in addition to active ingredients such as ginseng extract.

Here, "acceptable carrier in cosmetic preparation" means a compound or composition already known and used that may be included in a cosmetic preparation or a compound or composition which will be developed in the future and has no toxicity, instability or irritation more than the body can adapt to in contact with the skin. Say that.

The carrier may be included in the cosmetic composition for skin whitening of the present invention at about 1% by weight to about 99.99% by weight, preferably about 50% to about 99% by weight of the composition.

However, since the ratio is dependent on the form of the above-mentioned as the cosmetic composition of the present invention is prepared and its specific application site (face or hand) or its preferred application amount, the ratio is in any aspect of the present invention. It should not be understood to limit the scope.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

On the other hand, the skin lightening composition of the present invention can be regarded as a pharmaceutical composition in another specific embodiment.

When the composition for skin whitening of the present invention is identified as a pharmaceutical composition, the pharmacological effect may be understood as a treatment or prevention effect for a disease resulting from an increase in melanin pigment, and the skin whitening is for the treatment or prevention of the disease. It can be understood as a result of.

Here, the disease resulting from the increase of the melanin pigment is understood to include diseases which will be revealed in the future as well as diseases which have been discovered so far for the aforementioned reasons.

Nevertheless, the disease resulting from the increase of the melanin pigment preferably means a disease that has been discovered to date, and more preferably, a disease as exemplified above, among other known diseases, that is, blemishes, freckles, and skin aging.

The pharmaceutical compositions of the present invention may be administered via a variety of routes including oral, transdermal, subcutaneous, intravenous or intramuscular during clinical administration. In addition, the pharmaceutical composition of the present invention may be formulated in various formulations, and when formulated, it may be formulated using conventional diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants. . Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose, lactose and gelatin, etc. in ginseng extract. Mix is prepared. Lubricants may also be used in addition to simple excipients. Liquid preparations for oral administration include suspensions, solvents, emulsions, and syrups.In addition to the commonly used simple diluents, water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives, etc. May be included. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, Whitepsol, macrogol, tween 61, cacao butter, laurin butter, glycerol, gelatin and the like can be used.

The daily dosage of the pharmaceutical composition of the present invention is usually in the range of 0.1 to 150 mg / kg body weight, it may be administered once or divided into several times. However, since the actual dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage is in any aspect the scope of the present invention. It should not be understood as limiting.

The skin whitening composition of the present invention may be regarded as a food composition in another specific embodiment.

The food composition for skin whitening of the present invention may be prepared from gums, vitamin complexes, health supplements, special nutritional supplements, functional drinks, and the like.

In addition to the ginseng extract and the like, the food composition of the present invention, glucose, monosaccharides such as fructose, disaccharides such as maltose, sucrose, or polysaccharides such as dextrin, cyclodextrin may be added, xylitol, sorbitol, erythrate Sugar alcohols such as itol may also be added, sweeteners such as taurine, stevia extract and the like may also be added, and other food additives may be added.

The skin whitening composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition for skin whitening of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by including ginseng extract and the like on a soap base, and is prepared by including a skin moisturizer, an emulsifier, a hardener, and the like as an additive. Can be.

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion blocking agent, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally 99.9 to 50 wt% based on the total content of the soap composition. It may be added, the additive may be added in 1% to 20% by weight.

Here, the content of the ginseng extract and the like contained in the soap composition is effective as described above in the composition for skin whitening of the present invention.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to such examples and experimental examples.

<Example> Preparation of Ginseng Extract and Bamboo Extract

Example 1 Preparation of Ginseng Extract

The dried ginseng was sliced to have a particle size of 1 to 3 cm, and 100 g of the sliced ginseng was immersed in 3 L of 70% ethanol (a mixed solvent of ethanol and water), and extracted with stirring at room temperature for about 1 week. The extract was filtered with filter paper, and then the extraction solvent was removed from the filtered extract to finally prepare ginseng extract.

Example 2 Preparation of Bamboo Extract

The bamboo was chopped to a length of 1 to 5 cm, and then the chopped bamboo was immersed in water and extracted while heating and stirring at 90 ° C to 100 ° C for 48 hours. The extract was filtered with filter paper, and then the extraction solvent was removed from the filtered extract to finally prepare a bamboo extract.

Experimental Example Whitening effect experiment

Experimental Example 1 Tyrosinase Activity Inhibition Effect Experiment

The tyrosinase activity inhibitory effect of the extract obtained in each of the above examples and mixtures thereof was measured. Inhibition of tyrosinase activity was used by modifying the method of dopa oxidase. First, a mixture of the same amount of each extract obtained in <Examples 1 and 2> and the ginseng extract of <Example 1> and bamboo extract of <Example 2> was diluted in a buffer solution and prepared at a concentration of 50 µg / ml. It was. 40 μl of the prepared sample was mixed with 40 μl of 125 U / ml tyrosinase (Sigma) and 80 μl of 67 mM phosphate buffer (pH 6.8) in 25 mM L-dopa (3,4-dihydroxyphenylalamine). Into a test tube containing 40 μl and reacted at 37 ° C. for 60 minutes, the test tube was quenched to stop the reaction, and the absorbance of topachrome was measured at a wavelength of 475 nm with a spectrophotometer. As a control, a buffer solution was used instead of the extract of Example.

The activity inhibition rate of tyrosinase was calculated according to the following formula using the difference in absorbance and the results are shown in Table 1 below.

% Inhibition of activity of tyrosinase = 100- [absorbance of sample / absorbance of control X 100]

Inhibition rate of tyrosinase activity sample % Inhibition of tyrosinase activity Ginseng Extract of <Example 1> 56 Bamboo Extract of <Example 2> 32 Ginseng Extract of <Example 1> + Bamboo Extract of <Example 2> 82

Table 1 shows that each of the extracts of the above examples had inhibitory activity of tyrosinase, but in the case of the mixture of ginseng extract of <Example 1> and bamboo extract of <Example 2>, It has been shown to have inhibitory activity.

Experimental Example 2 Melanin Synthesis Inhibitory Effect Experiment

The inhibitory effect of melanin synthesis of the same amount of the mixture of each extract obtained in <Examples 1 and 2> and the ginseng extract of <Example 1> and the bamboo extract of <Example 2> was observed in B-16 melanoma cells ( B-16 mouse melanoma cells).

Melanosite was purchased from a mouse-derived B-16 melanoma cell line. Α-MSH was dissolved in 10% dimethyl sulfoxide (DMSO) and used as a 1 mM solution. Melanoma cell lines were inoculated in DMEM medium containing 4.5 g / L glucose, 10% serum and 1% antibiotics and incubated at 37 ° C. in a 75 cm 2 T-flask. After 24 hours of incubation under 5% CO 2 conditions, cells were treated with 0.05% Trypsin containing 0.02% EDTA, inoculated in a 75 cm 2 T-flask and incubated for 6 hours. Wherein the cell number is 1 ㅧ 10 6 cells / flask. Each sample was diluted with DMEM medium so that the final concentration was 50 μg / ml, and the cells were incubated at 37 ° C. for 5 days with treatment with an appropriate concentration of α-MSH. After incubation, the cells were treated with 0.05% trypsin containing 0.02% EDTA to separate cells, and then centrifuged for 5 minutes to collect cell pellets. The recovered pellets were transferred to an effendrof tube, centrifuged at 10,000 rpm for 10 minutes, and the supernatant was removed. The pellet from which the supernatant was removed was dried at 60 ° C. After drying, 200 µl of 1N NaOH was added to the pellet to dissolve melanin at 80 ° C. The dissolved melanin was diluted with PBS in an appropriate amount, and absorbance was measured at 490 nm with an ELISA reader (UV max, MOLECULAR DEVICES, USA), and the inhibition rate of melanin synthesis of each extract was expressed as a percentage (%) according to the following formula. . The control group is the experimental group which did not process any sample.

Melanin synthesis inhibition rate (%) = 100- [absorbance of sample / absorbance of control X 100]

Inhibition of Synthesis of Melanin sample Melanin synthesis inhibition rate (%) Ginseng Extract of <Example 1> 47 Bamboo Extract of <Example 2> 35 Ginseng Extract of <Example 1> + Bamboo Extract of <Example 2> 83

<Table 2> is similar to <Table 1>, but the extracts of each of the examples have the activity of inhibiting melanin synthesis, but in the case of the mixture of ginseng extract of <Example 1> and bamboo extract of <Example 2> In particular, it has been shown to have a high melanin synthesis inhibitory activity.

As described above, according to the present invention can provide a composition for skin whitening containing ginseng extract and bamboo extract.

Claims (12)

Skin whitening composition comprising one or more extracts selected from the group consisting of ginseng extract and bamboo extract as an active ingredient. The method of claim 1, The skin whitening composition is a result of skin melanin synthesis is a result of being inhibited. The method of claim 1, The skin whitening composition for skin whitening, characterized in that the skin aging, blemishes or freckle relief or improvement. The method of claim 1, The ginseng extract is a composition for skin whitening, which is obtained by extracting ginseng with ethanol, water, or a mixed solvent thereof. The method of claim 1, The bamboo extract is a skin whitening composition, characterized in that obtained by extracting bamboo with ethanol, water, or a mixed solvent thereof. The method of claim 1, The composition is a skin whitening composition comprising both ginseng extract and bamboo extract. A composition for skin whitening comprising at least one powder selected from the group consisting of ginseng powder and bamboo powder as an active ingredient. The method of claim 7, wherein The composition is a skin whitening composition comprising both ginseng powder and bamboo powder. The method according to any one of claims 1 to 8, The composition is a composition for skin whitening, characterized in that the cosmetic composition. The method according to any one of claims 1 to 8, The composition is a composition for skin whitening, characterized in that the soap composition. The method according to any one of claims 1 to 8, The composition is a composition for skin whitening, characterized in that the pharmaceutical composition. The method according to any one of claims 1 to 8, The composition is a composition for skin whitening, characterized in that the food composition.