KR20110118760A - Compositions for promotion of collagen synthesis - Google Patents

Abstract

PURPOSE: An external use skin composition containing marine algae extract for promoting collagen generation is provided to improve anti-aging or skin moisturizing. CONSTITUTION: An external use skin composition for promoting collagen contains 0.001-20. wt% of Carpopeltis cornea extract as an active ingredient. The extract is isolated using water, distilled water, ethanol, or a mixture solvent thereof. The cosmetic composition is manufactured in the form of an emulsion, lotion, cream, solution, suspension, anhydrous product(oil and glycols), gel, mask, pack, or powder. The composition is used for anti-wrinkling and skin moisturizing.

Description

Composition for external application for skin to promote collagen production {Compositions for Promotion of Collagen synthesis}

The present invention relates to an external preparation composition for promoting collagen production. Specifically, the present invention relates to a skin external preparation composition for promoting collagen production using extracts of seaweeds such as little hats only.

Collagen is an insoluble crosslinked protein produced and secreted by fibroblasts of the skin and is present in extracellular epilepsy and accounts for 30% of the total weight of biological proteins.

Collagen is a major fibrous protein that forms skin, bones, cartilage, blood vessels, etc.The main functions are collagen's mechanical strength, resistance of connective tissue and tissue binding, support of cell adhesion, induction of cell division and differentiation. It is known ( Ann NY Acad Sci . 1990; 580: 8-16).

In particular, collagen is composed of more than 90% of the dermal layer, when collagen is destroyed by ultraviolet rays, stress, active oxygen, etc., the skin elasticity is reduced and skin wrinkles occur ( Arch Dermatol . 1989; 125 (8): 1150). In addition, collagen has its own water retention, so when collagen is destroyed, the skin becomes dry and rough ( J Ginseng Res 31 (2): 86-92, 2007; Toxicol Appl Pharmacol 195: 298-308, 2004).

Thus, collagen biosynthesis promoting material may be useful as a cosmetic raw material to prevent skin wrinkles and improve the dryness of the skin.

For this reason, efforts have been made to find collagen biosynthesis promoting substances. For example, Korean Patent Publication No. 2009-0110726 discloses an ultrafiltration permeate of aloe vera gel having collagen production promoting activity, and Korean Patent Laid-Open Publication No. 2006-0037245 discloses a milk-derived basic protein fraction or excitation having collagen production promoting activity. Discloses a basic peptide fraction treated with protein hydrolase.

The present invention also discloses a seaweed extract having collagen production promoting activity.

An object of the present invention is to provide an external composition for skin for promoting collagen production.

Other objects or specific aspects of the present invention will be presented below.

The inventors of the present invention, as shown in the experimental example below, small algae, spiny skeletal net, reddish skin, wrinkled reddish leaf, sesame gambling, worms, wrinkled hair, black skin, 톳, etc. Treatment with CCD-986SK was confirmed to promote the biosynthesis of type I collagen (type I collagen). Type I collagen is a collagen that has been reported to be closely associated with skin elasticity and skin moisturization ( J. Inv . Der. , 98; 248-254, 1992; Arch Dermatol 138: 1462-1470, 2002). The collagen biosynthesis promoting effect of the algae extract in the following experimental example is not due to the cell growth promoting effect.

The present invention was completed based on the results of these experiments, the external composition for promoting the collagen production of the present invention is a little cap, spiny skeletal net, red skin, wrinkle red leaf, sesame gambling, worms, wrinkle wrinkled hair, black skin It is characterized in that it comprises an extract of seaweed selected from the group consisting of flesh and 으로 as an active ingredient.

In the present specification, the meaning of "promoting collagen production" includes the meaning of "for improving skin wrinkles" and / or "for moisturizing skin," as described above, since the amount of collagen has a close correlation with skin wrinkles and skin moisturizing. I mean. Therefore, it is understood that the skin external preparation composition for improving skin wrinkles comprising the algae extract as an active ingredient and / or the external skin composition for moisturizing the skin comprising the algae extract as an active ingredient is also included in the composition for promoting collagen production of the present invention. .

In the present specification, "algae extract" refers to a crude obtained by extracting the algae with methanol, distilled water, ethanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or a mixed solvent thereof regardless of the extraction method. It is understood as meaning including the extract obtained by fractionating the extract and its crude extract with the solvents listed above. Regardless of the extraction method, as long as the extraction object is extracted through the step of immersing in the extraction solvent, the extraction method may be any method such as cooling, refluxing, heating, ultrasonic radiation, and the like. Nevertheless, the "algae extract" is preferably extracted with algae to be extracted with water, distilled water, ethanol or a mixed solvent thereof, and the extraction solvent is removed to obtain a solid extract, and the solid extract is extracted with an aqueous layer and an ethyl acetate layer. Refers to the ethyl acetate layer fraction obtained after fractionation. Such extracts or fractions may be liquid concentrates or solids from which the extraction or fraction solvents have been removed.

In addition, the term "active ingredient" as used herein means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

Other terms that are not specifically defined herein follow the Korean dictionary meaning or the meaning generally accepted in the art.

Meanwhile, the composition for promoting collagen biosynthesis of the present invention may include seaweed extract, which is an active ingredient, in any amount (effective amount) according to the use, formulation, and mixing purpose, so long as it can exhibit collagen biosynthesis promoting activity. It will be determined within the range of 0.001% to 20.000% by weight based on the total weight of the composition. Here, "effective amount" refers to the amount of the active ingredient that can promote the biosynthesis of collagen. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

The external preparation composition for promoting collagen biosynthesis of the present invention can be understood as a cosmetic composition in a specific embodiment.

When the composition of the present invention is conceived as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and Aqueous), anhydrous products (oil and glycol based), gels, masks, packs, powders and the like.

The composition of the present invention may include an acceptable carrier in a cosmetic preparation in addition to including the active ingredient.

Herein, "acceptable carrier in cosmetic preparation" means a compound or composition already known and used that can be included in cosmetic preparations or a compound or composition which will be developed in the future and does not have more toxicity than the body can adapt to when contacted with the skin.

The carrier may be included in the composition of the present invention from about 1% to about 99.99% by weight, preferably from about 50% to about 99% by weight of the composition.

However, it is understood that the ratios limit the scope of the present invention in any aspect because the ratios vary depending on the formulation of the cosmetics as described above and their specific application site (face or hand) or their preferred application amount. It should not be.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

The composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by including an active ingredient thereof in a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a hard water softener, and the like as an additive. .

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti or the like may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion sequestrant, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally added in an amount of 99.999 wt% to 50 wt% based on the total weight of the soap composition. The additive may be added in an amount of 1% to 20% by weight.

The composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier, excipient, and the like in addition to the active ingredient, and may be a topical formulation such as creams, lotions, ointments (semi-solid external preparations), micro-emulsions, gels, pastes, transdermal preparations. (TTS) (such as patches, bandages, etc.) and the like.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) is not as toxic as applicable, without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, Solid lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, emulsifiers (such as TWEENS), wetting agents (Such as sodium lauryl sulfate), colorants, flavors, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions, and the like. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, suspending agents and dispersants such as sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethyl cellulose, sodium alginate, polyvinylpyrrolidone, etc. Can be.

The pharmaceutical composition of the present invention may be administered orally or parenterally, preferably topically.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

As described above, according to the present invention, it is possible to provide an external preparation composition for promoting collagen production. Collagen production promoting composition of the present invention can be used for skin wrinkle improvement or skin moisturizing use, it can be commercialized in cosmetics, soap and the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Preparation of Seaweed Extracts

[Table 1] Nine kinds of seaweeds were immersed in 80% ethanol and extracted for 24 hours at room temperature, followed by removal of the extraction residue, and concentration of dark sea to remove the extraction solvent to obtain a solid extract. 3 g of the solid extract of each seaweed was added to 300 ml of distilled water and 300 ml of ethyl acetate, and extracted at room temperature for 72 hours. The ethyl acetate layer was concentrated under reduced pressure and lyophilized. Each sample was aliquoted and dissolved in DMSO.

Seaweeds Used for Extraction Control Sample number sample Sargassum nipponicum JBR243 Little hat Dictyopteris prolifera JBR267 Barbed bone horse Prionitis cornea JBR268 Red black pepper Callophyllis crispata JBR354 Wrinkled red leaves Grateloupia elliptica JBR510 Gambling Sargassum thunbergii JBR511 Worm Chondrus crispus JBR514 Wrinkled hair Carpopeltis affinis JBR519 Black meat Hizikia fusiformis JBR538 톳

< Experimental Example > Collagen biosynthesis promoting activity experiment and skin wrinkle improvement clinical trial

< Experimental Example  1> Collagen Biosynthesis Promoting Activity Experiment

<1> cell culture

Human fibroblasts (CCD-986SK, human fibroblasts) used in the experiment were purchased from the American Type Culture Collection (ATCC , Rockville, USA), 10% FBS (fetal bovine serum, Hyclone) and 1% Antibiotic-Antimycotic ( Cultured at 37 ° C., 5% CO ₂ conditions with DMEM (Dulbecco's Modified Eagle Medium.Hyclone) added with Gibco).

<2> Type I collagen synthesis promoting effect experiment

Human fibroblasts were dispensed into 96 wells at a concentration of 2 × 10 ⁴ cells / well, and then cultured for 24 hours. Discard the medium and diluted the sample with serum free medium and put in 50㎍ / ml concentration and incubated for 24 hours. Ascorbic acid used as a positive control was treated at a concentration of 200 μM. Cultures were collected and used for experiments, and collagen synthesis in cell cultures was measured using a procollagen type I peptide EIA kit (Takara Biomedical Co.). 100 μl of Anticody-POD conjugate solution was added to each well, and then 20 μl of sample and standard were added and incubated at 37 ° C. for 3 hours. After the reaction, the culture solution was removed from each well and washed four times with 400 μl of PBS (phosphate buffered saline). 100 μl of substrate solution was added and reacted at room temperature for 15 minutes. After stopping the reaction by adding 100 μl of stop solution (1N H ₂ SO ₄ ), the absorbance was measured at 450 nm with an ELISA reader. A standard concentration curve was prepared to check the amount of collagen.

<3> cell viability evaluation experiment

Human fibroblasts are dispensed into 96 wells at a concentration of 2 × 10 ⁴ cells / well and incubated for 24 hours under cell culture conditions. Discard the medium and diluted the sample with serum free medium to 50 ㎍ / ml concentration and incubated for 24 hours. Ascorbic acid used as a positive control was treated at a concentration of 200μμM. MTT solution (2mg / ml 3- (4,5-dimethylthiazol-2-yl) -2,5 diphenyl-2H-tetrazolium bromid solution) was added to each well of 50ul and incubated for 4 hours. Remove the culture solution, add DMSO (dimethyl sulfoxide) solution, shake for 10 minutes, and measure absorbance at 540 nm with ELISA reader.

<4> results

The type I collagen production promoting effect test results and the cell survival rate evaluation test results are shown in Table 2 below.

Collagen synthesis promoting effect and cell survival rate Sample (50µg / ml) Collagen synthesis promoting effect (%) Cell survival rate (%) Control 100.0 100 Sargassum nipponicum JBR243 Little hat 163.6 ± 5.6 ** 75.2 Dictyopteris prolifera JBR267 Barbed bone horse 136.0 ± 2.4 ** 109.2 Prionitis cornea JBR268 Red black pepper 119.0 ± 3.7 ** 56.2 Callophyllis crispata JBR354 Wrinkled red leaves 124.9 ± 5.2 ** 64.2 Grateloupia elliptica JBR510 Gambling 145.1 ± 6.1 ** 74.2 Sargassum thunbergii JBR511 Worm 143.2 ± 3.9 ** 128.1 Chondrus crispus JBR514 Wrinkled hair 115.1 ± 1.8 ** 105.3 Carpopeltis affinis JBR519 Black meat 127.6 ± 2.5 ** 107.9 Hizikia fusiformis JBR538 톳 148.7 ± 8.2 ** 81.7 Ascorbic acid (200 uM) 140.1 ± 3.2 ** 107.7  * p <0.05, ** p <0.01

The results in Table 2 show that all nine seaweed extracts have a collagen biosynthesis promoting effect. This collagen biosynthesis promoting effect can be seen that the effect of the cell proliferation, as can be seen in the cell viability of the [Table 1].

Statistical processing

One way ANOVA test was used to determine the effect, and Dunnett's post hoc test was used to compare with the control group.

Claims (4)

Skin external preparation cosmetic composition for promoting the production of collagen containing the skin extract as an active ingredient.
The method of claim 1,
The extract is extracted with water, distilled water, ethanol or a mixed solvent thereof, and the extract extracts a solid extract to obtain a solid extract, and the solid extract is fractionated into an aqueous layer and an ethyl acetate layer to obtain ethyl. Cosmetic composition for external skin for promoting collagen production, characterized in that the acetate layer fraction.
The method of claim 1,
The skin external preparation composition for promoting collagen production is a skin external preparation cosmetic composition for promoting collagen production, characterized in that it is used for skin wrinkle improvement or skin moisturizing use.
Skin external preparation pharmaceutical composition for promoting the production of collagen containing the skin extract as an active ingredient.