CN110882195A - Collagen moisturizing and anti-wrinkle composition and preparation method thereof - Google Patents

Abstract

The invention belongs to the field of cosmetics, and particularly relates to a collagen moisturizing and anti-wrinkle composition and a preparation method thereof. The collagen moisturizing and anti-wrinkle composition provided by the invention comprises the following components in percentage by weight: 1.21-7.46% of humectant A, 1-4.43% of humectant B, 0.3-0.9% of preservative, 0.2-1% of antiallergic agent, 0.05-0.13% of thickening agent, 0.04-0.2% of pH regulator, 1-5% of 1, 2-hexanediol and 80.88-96.2% of water.

Description

Collagen moisturizing and anti-wrinkle composition and preparation method thereof

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a collagen moisturizing and anti-wrinkle composition and a preparation method thereof.

Background

Along with the improvement of the living quality of people and the pursuit of people for beauty, the cosmetic varieties are more and more, and the original deep nourishing care product of the facial mask becomes a daily product for skin care of people. The principle of the facial mask is that the facial mask is covered on the face for a short time, so that the external air and pollution are temporarily isolated, the skin temperature is increased, pores of the skin are expanded, the secretion and metabolism of sweat glands are promoted, the oxygen content of the skin is increased, the skin is favorable for removing products of epidermal cell metabolism and accumulated grease substances, and water and nutritional or functional substances in the facial mask permeate into the horny layer of the skin epidermis to soften the skin and make the skin naturally bright and elastic.

At present, commercially available masks are various in types and countless in types, but can be mainly divided into cleaning masks, moisturizing masks, soothing masks, firming masks, regenerating masks, whitening masks, moisturizing masks and anti-wrinkle masks according to different effects.

The Chinese patent application CN108451856A discloses a moisturizing mask and a preparation method thereof, the moisturizing mask utilizes iodopropynyl butyl carbamate and methylisothiazolinone to avoid breeding of bacteria in the mask, and has good effects of resisting allergy, diminishing inflammation, whitening, resisting oxidation, repairing skin, delaying senescence and the like.

Chinese patent application CN108245466A discloses a moisture-preserving anti-wrinkle mask, the mask liquid of the mask consists of an emulsifier, a humectant, a preservative, skin oil, a pH regulator and an anti-wrinkle repair substance, and although the mask has good short-time water replenishing and moisture-preserving effects, the long-acting moisture-preserving effect of the mask is general, the mask can only keep skin moisture within 2-3h and can not lock the moisture for a long time.

The problems that water replenishing, moisture retaining, wrinkle resisting and freckle removing can not be achieved simultaneously, toxic ingredients can be added in the preparation process of a mask liquid, and long-acting moisture retaining can not be achieved after the mask is used in the using process of the existing mask are to be solved urgently.

Disclosure of Invention

In order to solve the defects of the prior art, the invention provides a collagen moisturizing and anti-wrinkle composition and a preparation method thereof. The collagen moisturizing and anti-wrinkle composition provided by the invention adopts the mixture ratio of macromolecular sodium hyaluronate, glycerol, propylene glycol, erythritol and recombinant collagen as a moisturizing agent to perform long-acting moisturizing, and meanwhile, the preservative adopts the mixture ratio of jasminon and litchi leaf extract, and the antiallergic agent adopts the cooperation of folic acid fermentation product and melissa extract, so that the mask liquid has good long-acting moisturizing, antiseptic and antiallergic effects, and meanwhile, the mask liquid also has good effects of removing freckles, removing wrinkles and repairing skin.

The technical scheme of the invention is as follows:

the collagen moisturizing and anti-wrinkle composition is characterized by comprising the following components in percentage by weight:

1.21 to 7.46 percent of humectant A, 1 to 4.43 percent of humectant B, 0.3 to 0.9 percent of preservative, 0.2 to 1 percent of antiallergic agent, 0.05 to 0.13 percent of thickening agent, 0.04 to 0.2 percent of pH regulator, 1 to 5 percent of 1, 2-hexanediol and 80.88 to 96.2 percent of water;

the humectant A comprises the following components in parts by weight:

1-9 parts of macromolecular sodium hyaluronate, 600 parts of glycerol and 50-350 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

20-80 parts of erythritol and 50-400 parts of recombinant collagen.

Further, the collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%;

the humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate, 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

Furthermore, the preservative is composed of the jasminolone and the litchi leaf extract according to the weight ratio of 5-9: 1.

Further, the preservative is composed of the jasminolone and the litchi leaf extract according to the weight ratio of 7: 1.

Further, the antiallergic agent is composed of a folic acid fermentation product and a melissa extract according to a weight ratio of 2-4: 1.

Further, the antiallergic agent is composed of a folic acid fermentation product and a melissa extract according to a weight ratio of 3: 1.

Further, the thickening agent is carbomer 941; the pH regulator is triethanolamine.

The invention also provides application of the collagen moisturizing and anti-wrinkle composition in preparation of cold compress facial masks and moisturizing creams.

A preparation method of a collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1, 2-hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder for 12-24 h;

s2, adding macromolecular sodium hyaluronate, glycerol, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank filled with the carbomer 941 and 1,2 hexanediol soaked in the step S1, stirring, heating to 70-90 ℃, keeping the temperature for 30-50min, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 40-50 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5-10min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C prepared in the step S4 into the component B prepared in the step S3, stirring for 5min at a constant speed to obtain a mixed solution C with the pH value of 4.5-6.5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

The invention adopts the following raw material selective purchasing instructions:

the molecular weight of the macromolecular sodium hyaluronate is from 100 to 170 million Da, a breathable film can be formed on the skin epidermis to make the skin smooth and moist, and can block the invasion of external bacteria, dust and ultraviolet rays and protect the skin from being damaged; in short, the film-forming property is better, namely the water retention capacity is better.

The Xinxian ketone (p-hydroxyacetophenone) has the capabilities of resisting oxidation and stimulation, stabilizing emulsion and preventing broad-spectrum corrosion. The facial mask has good water solubility, overcomes the defect that a plurality of preservatives or preservative substitute products are not easy to add, has the effects of resisting stimulation and inhibiting inflammatory factors, and is a preservative substitute product because the preservative can be replaced in the facial mask.

The recombinant collagen is composed of monomers coded by a six-tandem repeat DNA sequence (EF376007), has the characteristics of a collagen domain of human type III collagen, is prepared by fermenting, inducing expression, performing eleven-pass separation and purification processes, filtering, sterilizing and freeze-drying in a GMP workshop by using genetic engineering bacteria constructed by a molecular biological method to obtain an active recombinant human collagen product, and has the following main characteristics:

1. the purity is high: single substance with purity as high as more than 99.5 percent; 2. the safety is high: the collagen has the same structure with human collagen, no animal-derived impurities, no allergy, no stimulation, no immune reaction and no virus hidden danger; 3. the molecular weight is definite: produced by engineering bacteria according to designed gene expression, and the molecular weight is 55.0 kDa; 4. the biocompatibility is good: has the characteristic of promoting the growth of cells, etc.

The folic acid fermentation product is purchased from Shanghai Qian-Chemicals Co., Ltd, is a Korea Duomei folic acid fermentation product, realizes the stability of folic acid for the soybean fermentation extracts polyglutamic acid and folic acid through a biological fermentation technology, has the functions of glutamic acid and folic acid, is easy to dissolve in water, has obvious effects of resisting allergy, repairing skin and removing wrinkles, and also has the effects of moisturizing and resisting aging.

The litchi leaf extract is obtained from Sasa veitchii Biotechnology GmbH, and is extracted from leaves of litchi of Sapindaceae by sun-drying and decocting, and has effects of removing dampness and removing toxic substance.

Melissa extract is obtained from Xian Tian biological technology corporation, and is the extract of pollen of Melissa, belonging to perennial herb of Labiatae, and has effects of tranquilizing mind, promoting digestion, relieving stress and tranquilizing mind.

Compared with the prior art, the invention has the following beneficial effects:

(1) the collagen moisturizing and anti-wrinkle composition provided by the invention adopts the moisturizing agent prepared by proportioning the macromolecular sodium hyaluronate, the medical glycerin, the propylene glycol, the erythritol and the recombinant collagen, so that the collagen moisturizing and anti-wrinkle composition has the effects of moisturizing, and can form a layer of protective film on the surface layer of skin to isolate pollutants, so that the effects of cleaning skin and keeping moisture for a long time are achieved, and meanwhile, the components are mutually proportioned, so that the collagen moisturizing and anti-wrinkle composition has a good whitening and repairing function, and the skin cell repair can be promoted;

(2) the collagen moisturizing and anti-wrinkle composition preservative adopts the mixture ratio of the jasminolone and the litchi leaf extract, meanwhile, the addition of the 1, 2-hexanediol not only preserves moisture, but also has a preservative effect, the dosage of the preservative is reduced, the toxicity is reduced, the preservative effect of the preservative is not weakened, the adopted litchi leaf extract has the effects of sterilizing, diminishing inflammation and preventing rot, and the rapid recovery of skin injury parts can be promoted while the preservative effect is prevented;

(3) the collagen moisturizing and anti-wrinkle composition disclosed by the invention adopts the folic acid fermentation product and the melissa extract to be matched for use, so that the collagen moisturizing and anti-wrinkle composition has a good anti-allergic effect, is suitable for any skin type, and the melissa extract is added, so that the fragrance is increased, and the effects of soothing nerves and promoting sleep are achieved;

(4) the humectant, the preservative, the antiallergic agent, the thickener and the pH regulator in the collagen moisturizing and anti-wrinkle composition are mutually coordinated, and the collagen moisturizing and anti-wrinkle composition has good effects of long-acting moisturizing, freckle removing, anti-wrinkle and skin repair.

Detailed Description

The present invention will be described in further detail with reference to the following examples. It should not be understood that the scope of the above-described subject matter of the present invention is limited to the following examples.

Example 1 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

1.21% of humectant A, 1% of humectant B, 0.3% of preservative, 0.2% of antiallergic agent, 0.05% of thickening agent, 0.04% of pH regulator, 1% of 1, 2-hexanediol and 96.2% of water.

The humectant A comprises the following components in parts by weight:

1 part of macromolecular sodium hyaluronate (with the molecular weight of 100 ten thousand Da), 100 parts of glycerol and 50 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

20 parts of erythritol and 50 parts of recombinant collagen.

The preservative consists of the jasminonone and the litchi leaf extract according to the weight ratio of 5: 1.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 2: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1, 2-hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate (molecular weight of 100 ten thousand Da), medical glycerin, jasminodone, litchi leaf extract and propylene glycol into an emulsifying cylinder containing carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying cylinder reaches 80 ℃, then opening an emulsifying cylinder cooling system, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 4.5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Example 2 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

7.46 percent of humectant A, 4.43 percent of humectant B, 0.9 percent of preservative, 1 percent of antiallergic agent, 0.13 percent of thickening agent, 0.2 percent of pH regulator, 5 percent of 1, 2-hexanediol and 80.88 percent of water.

The humectant A comprises the following components in parts by weight:

19 parts of macromolecular sodium hyaluronate (with the molecular weight of 170 ten thousand Da), 600 parts of glycerol and 350 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

80 parts of erythritol and 400 parts of recombinant collagen.

The preservative consists of jasminonone and litchi leaf extract according to the weight ratio of 9: 1.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 4: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6.5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Example 3 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A4.29%, humectant B3.1%, antiseptic 0.9%, antiallergic agent 1%, thickener 0.13%, pH regulator 0.2%, 1, 2-hexanediol 3.5% and water 86.88%.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 140 ten thousand Da), 200 parts of glycerol and 210 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

45 parts of erythritol and 250 parts of recombinant collagen.

The preservative consists of jasminonone and litchi leaf extract according to the weight ratio of 6: 1.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 4: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C prepared in the step S4 into the component B prepared in the step S3, stirring for 5min at a constant speed to obtain a mixed solution C with the pH value of 5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Example 4 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 120 ten thousand Da), 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

The preservative consists of jasminonone and litchi leaf extract according to the weight ratio of 7: 1.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 3: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 1 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%.

The humectant A comprises the following components in parts by weight:

305 parts of glycerol and 200 parts of propylene glycol;

the humectant B is recombinant collagen.

The preservative consists of jasminonone and litchi leaf extract according to the weight ratio of 7: 1.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 3: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding glycerol, jasminodone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, keeping the temperature for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding the bee pollen extract, the folic acid fermentation product and the recombinant collagen into the component A obtained in the step S2, stirring for 5min at a constant speed to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 1 is substantially the same as example 4, with the main difference that comparative example 1 replaces the macromolecular sodium hyaluronate with the same weight component of glycerol and the same weight component of recombinant collagen instead of erythritol.

Comparative example 2 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 120 ten thousand Da), 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

The preservative consists of the jasminonone and the litchi leaf extract according to the weight ratio of 1: 1.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 3: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 2 is substantially the same as example 4 except that the weight ratio of the preservative jasmone and the litchi leaf extract of comparative example 2 is changed from 7:1 to 1: 1.

Comparative example 3 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 120 ten thousand Da), 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

The antiseptic is menthone.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 3: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 2 is substantially the same as example 4 except that the preservative of comparative example 2 is jasmone.

Comparative example 4 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 120 ten thousand Da), 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

The preservative is litchi leaf extract.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 3: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 2 is essentially the same as example 4, except that the preservative of comparative example 2 is litchi leaf extract.

Comparative example 5 preparation of collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 120 ten thousand Da), 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

The preservative consists of jasminonone and litchi leaf extract according to the weight ratio of 7: 1.

The antiallergic agent is folic acid fermentation product.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin, jasminorange ketone, litchi leaf extract and propylene glycol into an emulsifying tank filled with the step S1 of soaking 941 carbomer and 1,2 hexanediol, heating to 80 ℃ at a stirring speed of 25r/min, preserving heat for 20min when the temperature of the emulsifying tank reaches 80 ℃, then opening a cooling system of the emulsifying tank, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, a folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed unchanged, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring for 5min at a constant speed to obtain a mixed solution C having a pH of 6;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 3 is substantially the same as example 4 except that the antiallergic agent of comparative example 3 is a single-component folic acid fermentation product.

Comparative example 6 collagen moisturizing and anti-wrinkle composition

The collagen moisturizing and anti-wrinkle composition comprises the following components in percentage by weight:

4.29 percent of humectant A, 3.1 percent of humectant B, 12.5 percent of preservative, 1 percent of antiallergic agent, 0.13 percent of thickening agent, 0.2 percent of pH regulator, 3.5 percent of 1, 2-hexanediol and 86.88 percent of water.

The humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate (with the molecular weight of 140 ten thousand Da), 200 parts of glycerol and 210 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

45 parts of erythritol and 250 parts of recombinant collagen.

The antiallergic agent consists of folic acid fermentation product and melissa extract according to the weight ratio of 4: 1.

The thickening agent is carbomer 941; the pH regulator is triethanolamine.

The preparation method of the collagen moisturizing and anti-wrinkle composition comprises the following steps:

s1, adding carbomer 941 and 1,2 hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder with the capacity of 100kg for 24 hours;

s2, adding macromolecular sodium hyaluronate, medical glycerin and propylene glycol into an emulsifying cylinder containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, heating to 80 ℃ at a stirring speed of 25r/min, preserving the temperature for 20min when the temperature of the emulsifying cylinder reaches 80 ℃, then opening a cooling system of the emulsifying cylinder, keeping the stirring speed unchanged, and cooling to 45 ℃ to obtain a component A;

s3, sequentially adding erythritol, melissa extract, folic acid fermentation product and recombinant collagen into the component A obtained in the step S2, keeping the speed constant, and stirring for 5min to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C prepared in the step S4 into the component B prepared in the step S3, stirring for 5min at a constant speed to obtain a mixed solution C with the pH value of 5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.

Comparative example 6 is essentially the same as example 3, except that the preservative is 1,2 ethylene glycol.

Test example I, skin irritation test

1. Test materials: collagen moisturizing anti-wrinkle compositions prepared in examples 1 to 4 and comparative examples 1 to 5.

2. Test subjects: 90 female volunteers were selected, aged 25-50 years.

3. The test method comprises the following steps: the collagen moisturizing and anti-wrinkle compositions obtained in examples 1 to 4 and comparative examples 1 to 5 were applied to the face of the subject, and the results were observed after 15 min.

4. And (3) test results: the observation shows that no irritant reaction such as local redness and eruption occurs in 90 subjects, which shows that the collagen moisturizing anti-wrinkle composition is safe and non-irritant and has no adverse reaction.

Test example two, moisture retention anti-wrinkle energy efficiency test

1. Test materials: the collagen moisturizing and anti-wrinkle compositions prepared in examples 1 to 4 and comparative examples 1 to 5.

2. The test method comprises the following steps: the relative moisturizing rate, the skin water content, the heavy metal content and the wrinkle relieving degree of the tested collagen moisturizing and wrinkle resisting composition are respectively tested.

3. The experimental results are shown in table 1, wherein the content of heavy metal is as follows: mercury is less than or equal to 1mg/kg, arsenic is less than or equal to 10mg/kg, and lead is less than or equal to 40 mg/kg.

Table 1 moisturizing and wrinkle-resistant energy efficiency test results

As can be seen from table 1, the collagen moisturizing and anti-wrinkle compositions prepared in examples 1 to 4 have higher relative moisturizing rate, skin moisture content, lower heavy metal content, and better anti-oxidation and facial wrinkle soothing properties than the collagen moisturizing and anti-wrinkle compositions prepared in comparative examples 1 to 5, wherein the collagen moisturizing and anti-wrinkle composition prepared in example 4 has the best combination properties, and is the best embodiment of the invention.

The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (10)

1. The collagen moisturizing and anti-wrinkle composition is characterized by comprising the following components in percentage by weight:

1.21 to 7.46 percent of humectant A, 1 to 4.43 percent of humectant B, 0.05 to 0.13 percent of thickening agent, 0.04 to 0.2 percent of pH regulator, 1 to 5 percent of 1, 2-hexanediol and the balance of water;

the humectant A comprises the following components in parts by weight:

1-9 parts of macromolecular sodium hyaluronate, 600 parts of glycerol and 50-350 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

20-80 parts of erythritol and 50-400 parts of recombinant collagen.

2. The collagen moisturizing and anti-wrinkle composition according to claim 1, which comprises the following components in percentage by weight:

humectant A5.05%, humectant B3.6%, antiseptic 0.4%, antiallergic agent 0.5%, thickener 0.12%, pH regulator 0.12%, 1, 2-hexanediol 2% and water 88.21%;

the humectant A comprises the following components in parts by weight:

5 parts of macromolecular sodium hyaluronate, 300 parts of glycerol and 200 parts of propylene glycol;

the humectant B comprises the following components in parts by weight:

60 parts of erythritol and 300 parts of recombinant collagen.

3. The collagen moisturizing and anti-wrinkle composition according to claim 1 or 2, further comprising 0.3% to 0.9% of a preservative, wherein the preservative is composed of jasmone and litchi leaf extract in a weight ratio of 5-9: 1.

4. The collagen moisturizing and anti-wrinkle composition according to claim 3, wherein the preservative is composed of jasmone and litchi leaf extract in a weight ratio of 7: 1.

5. The collagen moisturizing and anti-wrinkle composition according to claim 1 or 2, further comprising 0.2% -1% of an anti-allergic agent, wherein the anti-allergic agent is composed of a folic acid fermentation product and a melissa extract in a weight ratio of 2-4: 1.

6. The collagen moisturizing and anti-wrinkle composition according to claim 5, wherein the antiallergic agent is composed of a folic acid fermentation product and a bee pollen extract in a weight ratio of 3: 1.

7. The collagen moisturizing anti-wrinkle composition according to claim 1 or 2, wherein the thickener is carbomer 941; the pH regulator is triethanolamine.

8. Use of the collagen moisturizing and anti-wrinkle composition as claimed in any one of claims 1 to 7 in preparation of a cold pack mask, a moisturizing cream.

9. The method for preparing the collagen moisturizing and anti-wrinkle composition according to any one of claims 1 to 7, comprising the steps of:

s1, adding carbomer 941 and 1, 2-hexanediol into water with the total water consumption of 95-99%, and soaking in an emulsifying cylinder for 12-24 h;

s2, adding the humectant A and the preservative into an emulsifying cylinder containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, stirring, heating to 70-90 ℃, keeping the temperature for 30-50min, then opening a cooling system of the emulsifying cylinder, keeping the stirring speed unchanged, and cooling to 40-50 ℃ to obtain a component A;

s3, sequentially adding the humectant B and the antiallergic agent into the component A obtained in the step S2, stirring for 5-10min at a constant speed to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C obtained in step S4 to the component B obtained in step S3, stirring at a constant speed to obtain a mixed solution C having a pH of 4.5 to 6.5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, filtering the qualified mask liquid through a filter screen, and sealing and storing to obtain the mask liquid.

10. The method for preparing the collagen moisturizing and anti-wrinkle composition according to claim 9, comprising the steps of:

s1, adding carbomer 941 and 1, 2-hexanediol into water with the total water consumption of 99%, and soaking in an emulsifying cylinder for 12-24 h;

s2, adding the humectant A and the preservative into an emulsifying cylinder containing the carbomer 941 and 1,2 hexanediol soaked in the step S1, stirring, heating to 70-90 ℃, keeping the temperature for 30-50min, then opening a cooling system of the emulsifying cylinder, keeping the stirring speed unchanged, and cooling to 40-50 ℃ to obtain a component A;

s3, sequentially adding the humectant B and the antiallergic agent into the component A obtained in the step S2, stirring for 5-10min at a constant speed to obtain a component B;

s4, dissolving the pH regulator triethanolamine in water according to the proportion to obtain a component C;

s5, adding the component C prepared in the step S4 into the component B prepared in the step S3, stirring for 5min at a constant speed to obtain a mixed solution C with the pH value of 4.5-6.5;

s6, uniformly stirring the mixed solution C prepared in the step S5, closing stirring to obtain a mask solution, sampling, detecting and reserving a sample;

and S7, passing the qualified facial mask liquid through a 200-mesh filter screen, and then sealing and storing to obtain the facial mask liquid.