KR20110081057A - Solid support comprising microorganisms capable of conducting parallel multiple mineralization immobilized thereon, catalyst column and method for producing solid medium for cultivating plants - Google Patents

Abstract

An object of the present invention is to provide a method for efficiently producing nitrate nitrogen, which is an inorganic fertilizer component, from an organic material without performing an operation using constant power such as aeration. It is another object of the present invention to provide a method for producing a plant-cultured solid medium, which is capable of directly cultivating a nutrient solution even when nutrient solution cultivation is carried out in a solid gypsum. Adding a microorganism group for carrying out a simultaneous doubling mineralization reaction in which a container is filled with a solid carrier having permeability; and an inorganic substance is added to the container so as to produce nitrate nitrogen, followed by addition of an organic substance, And the solid carrier is allowed to flow out of the solid carrier to allow the nitrate nitrogen to be produced in the effluent to be started to be formed, thereby fixing the microorganism group to be subjected to the simultaneous doubly mineralization reaction, A method for producing a solid support on which a group of microorganisms to be reacted is immobilized.

Description

TECHNICAL FIELD [0001] The present invention relates to a solid support, a catalyst column, and a method for producing solid medium for plant cultivation, in which a microorganism group for performing a parallel doubly-mineralization reaction is immobilized }

The present invention relates to a method for producing a solid carrier immobilized with a microorganism group which performs nitric acid nitrogen production by mineralizing organic matters and which performs a mineralization reaction.

The present invention also relates to a method for producing a catalyst column for producing nitrate nitrogen, which is an inorganic fertilizer component, from an organic material using the solid carrier. The present invention also relates to a method for producing a plant-cultured solid medium, wherein the solid carrier is a plant-grown solid medium.

Recently, from the viewpoint of establishing a recycling society, the movement of using organic fertilizer to refrain from the use of chemical fertilizer has become active worldwide.

However, in the case of 'nutrient solution cultivation' in which soil is not used, which is diffused in the production of vegetables such as tomatoes and flowers, direct addition of organic matter to the nutrient solution causes harmful intermediate decomposition products, The use of organic fertilizer in nutrient solution cultivation could not be considered. Therefore, only chemical fertilizer is still used in nutrient solution cultivation.

To utilize organic fertilizer in nutrient solution cultivation, it is necessary to make a technique to mineralize organic matter into a useful inorganic fertilizer component such as nitrate nitrogen. Conventionally, as a technique for mineralizing organic matter, there is a drainage treatment technique using a microorganism group (see, for example, Patent Document 1), but they involve a denitrification reaction in which the produced nitrate nitrogen is reduced and released as nitrogen gas As a result, the nitrate nitrogen is lost, which is not consistent with the purpose of producing inorganic fertilizer.

As a technology capable of efficiently recovering nitrate nitrogen (as nitrate ion) from an organic material and using it as an inorganic fertilizer component, Patent Document 2 and Non-Patent Document 1 described in Patent Document 1 have been proposed.

This technique is a highly reproducible method that can decompose organic nitrogen while suppressing denitrification and can recover nitrate ions as nitrate ions as inorganic fertilizer components with high efficiency. This has made it possible to "cultivate nutrient solution utilizing organic fertilizer" and "manufacture of inorganic fertilizer containing nitrate nitrogen with organic matter as a raw material" (see, for example, Non-Patent Documents 1 and 3 Reference).

The invention described in Patent Document 2 attracts attention as a technique for realizing nutrient solution cultivation using an organic fertilizer and also for producing an inorganic fertilizer component such as nitrate nitrogen using organic resources as a raw material. For this reason, farmers and plant factories interested in new nutrient cultivation techniques are attracting much attention from companies planning to recycle organic resources.

In addition, by using the parallel doubled mineralization method described in Patent Document 2, organic fertilizer can be directly added to the nutrient solution to produce nitrate nitrogen, and it becomes possible to perform "nutrient solution cultivation in hydroponics using organic fertilizer".

However, the method of producing a fertilizer containing an inorganic fertilizer component by the parallel doubling method described in Patent Document 2, the nitrification property in decomposition (ammonia-forming property) of organic matter and nitrate ion production (nitrifying property) The surface area of the wall surface is rate-controlled, and there is a problem that the time is taken too much in terms of the reaction rate of mineralizing the organic matter into nitrate nitrogen.

In addition, the cultivation and the reaction of the microorganism group require constant aeration (operation for always maintaining the aerobic conditions by aeration), and when the large-scale treatment is assumed, the possibility that the power cost becomes a problem . Therefore, there has been a demand for development of a method capable of producing nitrate nitrogen, which is an inorganic fertilizer component, from an organic material, in which the reaction rate is remarkably high (efficient) and can be carried out without requiring aeration or constant power .

However, when nutrient solution cultivation using a solid medium such as rock wool or the like, which is frequently used in tomato cultivation, or the like ("cultivation of a nutrient solution in a solid culture medium") is carried out, when the organic fertilizer is directly added to the nutrient solution, Decomposition does not proceed smoothly and is corrupted, so that there is a problem that nitrate nitrogen is hardly produced (see, for example, Non-Patent Document 2).

Thus, although the use of the parallel doubled inorganicization method described in Patent Document 2 has been studied, even in this case, if the organic fertilizer is directly added to the nutrient solution, the organic component is dissolved in the nutrient solution, There is a possibility that problems such as clogging may occur and decay of organic matter causing clogging in a solid medium may occur, and there is a problem that application is difficult in this state.

Therefore, in order to perform the solidification diameter by using the method described in Patent Document 2, unless the organic matter is fully mineralized in advance and made into an inorganic nutrient solution (a nutrient solution which does not contain an organic component as much as possible) I could not use it.

Therefore, in this case, the preparatory operation before cultivation is cumbersome and time consuming, so that development of a technology that makes it possible to utilize the organic fertilizer in a solid gaseous dispersion by a simpler method has been demanded.

In addition, although a conventional method of fixing microorganisms to a solid medium (see, for example, Patent Document 1) has been proposed, they are accompanied by a denitrification reaction and lose the fertilizer component (nitrate nitrogen) It could not be used as a solid medium for nutrient solution cultivation.

Patent Document 1: Japanese Patent Application Laid-Open No. 2001-300583

Patent Document 2: Japanese Patent Application Laid-Open No. 2007-119260

Non-Patent Document 1: "Nutrient Solution Cultivation of Organic Fertilizer", Agriculture and Horticulture, vol. 81, p. 753-764 (2006)

Non-Patent Document 2: " Quantitative Soil Sludge, Liquid Manure, and Badge Management " Hakuchi, p.119-155 (2005)

Non-Patent Document 3: "Production of inorganic fertilizer for export from food wastes and manure" Journal of Agricultural and Fisheries Technology, vol.31, p.44-46 (2008)

SUMMARY OF THE INVENTION It is an object of the present invention to provide a method for effectively producing nitrate nitrogen, which is an inorganic fertilizer component, from an organic matter without performing an operation using constant power such as aeration.

It is another object of the present invention to provide a method for producing a plant-cultured solid medium, which is capable of directly cultivating a nutrient solution even when nutrient solution cultivation is carried out in a solid gypsum.

The present inventors have used a microorganism group for carrying out a simultaneous doubling mineralization reaction in which organic matters are mineralized to produce nitrate nitrogen (nitrate nitrogen), which is immobilized on a solid carrier having air permeability and used as a carrier of a catalyst column It is possible to remarkably improve the reaction rate of the parallel doubly-charged inorganic reaction without performing any operation using constant power such as aeration and to find out that nitrate nitrogen as an inorganic fertilizer component can be efficiently produced from organic matter Respectively.

Further, the inventor of the present invention found that even when the solid carrier is used as a plant-cultivated solid medium, even when nutrient solution cultivation is carried out on a solid gypsum, the organic fertilizer can be directly added and the nutrient solution cultivation can be carried out Respectively.

The present invention has been completed on the basis of these findings.

That is, the invention of claim 1 is characterized in that a container is provided with a solid carrier having air permeability, and a microorganism group for carrying out a parallel-doubling mineralization reaction in which an organic substance is mineralized to produce nitrate nitrogen is added to the container; And thereafter adding water and allowing the solid carrier to flow out of the solid carrier to wash the solid carrier until the nitrate nitrogen starts to be produced in the effluent, thereby immobilizing the microorganism group that performs the parallel double mineralization reaction Wherein the microorganism group is subjected to a simultaneous doubly mineralization reaction.

The invention of claim 2 is a method for producing a solid carrier according to claim 1, wherein the container is a container having a drain hole, and the effluent is an effluent discharged from the drain hole.

According to a third aspect of the present invention, there is provided a method of manufacturing a solid carrier, comprising: adding a microorganism group that performs nitric acid nitrogen formation by mineralizing organic matter to perform a mineralization reaction; And thereafter adding water and allowing the solid carrier to flow out of the solid carrier to wash the solid carrier until the nitrate nitrogen starts to be produced in the effluent; Wherein the microorganism group is subjected to a simultaneous doubly mineralization reaction.

The invention according to claim 4 is characterized in that when nitrate nitrogen is not started to be produced in the effluent flowing out when the solid carrier is washed, then the organic matter is added, and then the mixture is allowed to stand, Wherein the treatment for washing the solid carrier is repeatedly carried out until nitrate nitrogen starts to be produced in the effluent flowing out during the washing.

The invention of claim 5 is the production method of a solid carrier according to any one of claims 1 to 4, wherein the above step is carried out until nitric acid ions of 50 mg / L or more start to be produced in the effluent.

The present invention according to claim 6 is characterized in that the solid carrier is selected from the group consisting of a rock solid, a vermiculite, a pearlite, a zeolite, a sand, a gumumato, a glass, a ceramic, a urethane, a nylon, a melamine resin, a wood chip, Wherein the solid carrier is at least one porous solid carrier selected from the group consisting of a charcoal, a cotton, a paper, a polyacrylamide gel, and agar.

According to a seventh aspect of the present invention, there is provided a method for producing a solid support, comprising: adding, immediately after adding the microorganism group, nitric acid nitrogen of 10 mg or more in nitrate ion conversion to 1 L of the solid carrier in the solid carrier; And removing nitrate nitrogen from the solid carrier, wherein the ammonia-forming reaction and the nitrifying reaction proceed without accumulation of the intermediate product in the solid carrier when the organic substance is added, A solid carrier production method according to any one of claims 1 to 6, wherein no denitrification reaction occurs.

The invention according to claim 8 is characterized in that the microorganism group that performs the parallel doubly mineralization reaction is a culture solution in which a microorganism group for performing a parallel doubly mineralization reaction is cultured, a dried cell of the microorganism obtained by drying the culture solution, , 1) selected from the group consisting of effluents, soil, tap water, lake water, spring water, well water, river water and seawater discharged from the solid carrier by adding water to the solid carrier immobilized with the microorganism group, Wherein the solid support is derived from a microorganism source comprising a microorganism group that performs ammonia formation and a microorganism group that performs nitrification property.

The invention of claim 9 is the process for producing a solid carrier according to any one of claims 1 to 8, wherein the amount of the organic substance is 0.1 to 10 g with respect to 1 L of the carrier.

The invention of claim 10 is the method for producing a solid carrier according to any one of claims 1 to 9, wherein the organic substance is a nitrogen-containing organic substance having a carbon / nitrogen content ratio C / N ratio of 19 or less.

The invention according to claim 11 is characterized in that the high nitrogen-containing organic material according to claim 10 is at least one selected from the group consisting of fish boiled soup, fish meal, oil cake, food waste, corn steep liquor, rice bran, soybean meal, And at least one member selected from the group consisting of animal fats and oils.

The invention of claim 12 is the use of the solid carrier described by the method according to any one of claims 1 to 11 as a carrier of a catalyst column for carrying out the simultaneous double- In the presence of a catalyst.

The invention of claim 13 is a fertilizer containing nitrate nitrogen which is an inorganic fertilizer component produced by using the catalyst column obtained by the method according to claim 12.

The invention of claim 14 is a method of cultivating a plant using the nitrate nitrogen-containing fertilizer according to claim 13.

The invention of claim 15 is a method for producing a plant-grown solid medium, characterized in that the solid carrier obtained by the method according to any one of claims 1 to 11 is used as a plant-cultured solid medium.

The invention of claim 16 is the plant-grown solid medium obtained by the invention of claim 15.

The invention of claim 17 is a cultivation method of a plant in which organic fertilizer is directly added and cultivation is carried out by using the plant-cultivated solid medium according to claim 16.

The invention of claim 18 is the method according to any one of claims 14 or 17, wherein the plant is a method for cultivating vegetable, vegetable, flower, tree, or fruit plant for harvesting vegetables, fruits of leafy vegetables.

The present invention makes it possible to provide a catalyst column capable of remarkably improving the reaction rate of the parallel double mineralization reaction and efficiently producing nitrate nitrogen as an inorganic fertilizer component from organic matter.

Thus, it is an object of the present invention to efficiently produce a fertilizer containing nitrate nitrogen, which is an inorganic fertilizer ingredient, from an organic material, without using an organic power source or an organic fertilizer as a raw material, .

Further, the present invention makes it possible to directly add organic fertilizer to conduct nutrient solution cultivation even in the case where the nutrient solution cultivation is carried out in a solid size gage.

The present invention relates to a method for producing a solid carrier immobilized with a microorganism group which performs nitric acid nitrogen production by mineralizing organic matters and which performs a mineralization reaction.

The present invention also relates to a method for producing a catalyst column for producing nitrate nitrogen, which is an inorganic fertilizer component, from an organic material using the solid carrier. The present invention also relates to a method for producing a plant-cultured solid medium, wherein the solid carrier is a plant-grown solid medium.

1 is an explanatory view showing an embodiment of a method for immobilizing a microorganism group with a solid carrier. 2 is an explanatory view showing an embodiment of a catalyst column for generating nitrate nitrogen as an inorganic fertilizer component from organic matter. 3 (a) and 3 (b) are photographic top views showing a state in which a solid carrier is filled in a container provided with a drain port.

In the present invention, the production of a solid carrier immobilized with a microorganism group for performing a parallel-doubling mineralization reaction is carried out by charging a container with a solid carrier having permeability, and adding thereto a biodegradable inorganic mineralization reaction And then washing the solid carrier by adding water to the solid carrier and adding water to the solid carrier to remove nitrate nitrogen from the solid carrier, It is done by doing.

Further, in the present invention, when a solid carrier is integrally molded so as to maintain its solid shape, a solid carrier immobilized with a microorganism group for performing a parallel doubling mineralization reaction without charging the solid carrier into the container is used It is also possible to manufacture.

That is, a solid support having a breathability, which is integrally formed so as to maintain a solid shape, is prepared by adding to a solid carrier having air permeability, a microorganism group that performs nitrate nitrogen generation by nitrifying organic substances, , And adding water and allowing the solid carrier to flow out from the solid carrier, the solid carrier is allowed to stand until the nitrate nitrogen starts to be produced in the effluent.

The first step in the production of the solid carrier immobilized with the microorganism group for carrying out the parallel double ionization reaction of the present invention is first a step (filling step) of filling the container with the breathable solid carrier.

In addition, the solid carrier may be stacked (specifically, a solid carrier having air permeability is piled up on a flat structure such as an acid stacked on one side), and water is directly added to the solid carrier to be discharged It is possible to carry out the microorganism group addition step as the next step without carrying out the filling step for filling the container by stacking it with an acid that does not collapse when the microorganism is added.

Further, even when the solid carrier is integrally molded so as to maintain the solid shape, it is also possible to directly carry out the microorganism group addition step as the next step without carrying out the filling step of filling the container.

In the present invention, the solid carrier for immobilizing the microorganism group may be any microorganism, an organic component, and any substance that can carry moisture, has permeability, and is porous. Particularly, it is preferable that the material has a large surface area in which microorganisms are immobilized (fixed) to the volume.

Here, "having air permeability" means that the environment of voids in the solid carrier and / or the solid carrier when the solid carrier is packed in a column (column-like container) or stacked up to form an acid, The environment of the voids in the solid support is in a state in which the nitrification property is easy to proceed by keeping the aerobic conditions and the vapor phase in which the denitrification reaction is unlikely to occur is secured.

Hereinafter, the entire environment within the solid carrier and the solid carrier may be expressed simply as " the solid carrier " or " the solid carrier as a whole ".

Specific examples of such a solid carrier include materials of rock minerals such as rock wool, vermiculite, pearlite, zeolite, sand, cannum, glass (specifically glass beads) and ceramics, urethane, nylon, melamine resin, poly Based materials such as woody chips (specifically cedar chips), rice straw, conception, charcoal (specifically, activated carbon), cotton, paper (specifically, filter paper) and agar Etc. may be used.

In the present invention, it is preferable to use a rock surface, a vermiculite, a pearlite, a urethane, a cedar chip, a rice straw, an impregnated or fertilizer paper, and more preferably a rock surface or urethane.

In addition, when general soil is used as the solid carrier, addition of organic matter and water makes the microbes more prone to overproduce, so that the air permeability deteriorates, denitrification reaction by the denitrifying bacteria that reproduce in the soil occurs, and finally nitrate nitrogen Is not obtained, which is not preferable.

The solid carrier integrally molded so as to maintain the solid shape may be a solid carrier formed by a cube shape, a sphere shape, a cylindrical shape, a rod shape, or the like and the entire carrier is integrally formed, Molded.

Concretely, it is possible to use the thus shaped rock surface, vermiculite, pearlite, zeolite, sand, cannum, glass (specifically glass bead), urethane, nylon, melamine resin, wood chips (specifically, cedar chips) And can be handled as a solid carrier by itself as a material such as paper, impregnated paper, charcoal (specifically, activated charcoal), cotton, paper (specifically, filter paper), polyacrylamide gel and agar, A material capable of being molded can be used. It is preferable to use a rock surface.

The size of the solid carrier integrally molded so as to maintain the solid shape is not particularly limited as long as it has a volume of 10 ml or more.

The container for filling the solid carrier is a container capable of filling the solid carrier. Any container capable of allowing water to flow out after adding water can be used.

Preferably, it may be a container having a drain hole, a structure capable of efficiently discharging water after adding water (a structure capable of being used as a column after filling the solid carrier). Specifically, it is possible to use a cup-shaped upper opening, a column-shaped lower opening, or a container having a mesh-like shape as a whole.

Further, even if the container is not a container having a drain port, it can be used as a container capable of performing an operation of tilting the container to perform an operation (decantation) for discharging water.

The filling of the solid carrier into the container is carried out in such a manner that voids (vapor phase) in the solid carrier and / or in the solid carrier are ensured so that the whole is not flooded by capillary phenomenon after the water is added and flowed out . (H) (unit: m) of the liquid surface due to the capillary phenomenon is given by the following formula I. Moreover, each symbol in the formula I is, T = surface tension (N / m), θ = contact angle, ρ = density of the liquid (㎏ / ㎥), g = gravitational acceleration ^{(m / s 2), r} = tube (Radius) (m).

When the space between the solid carriers and / or the solid carrier becomes narrow and h exceeds the packed height of the solid carrier, even if the drain is opened in the case of adding water, all the carrier becomes fresh and becomes anaerobic, There is a fear that it will not progress sufficiently.

Therefore, it is preferable that the voids in the solid support and / or the solid support ensure a size such that h does not exceed the charge height, and it is preferable that h is smaller than the charge height of the solid support, not.

Most preferably, the pores in the solid carrier and / or the solid carrier are coarse to ensure air permeability in a portion having a large pore size and to maintain the permeability in a portion having a narrow pore size, (Water-retaining property), it is preferable to secure both the air permeability and the water retention as the whole carrier.

If the voids in the solid support and / or the solid support are narrow and the h exceeds the fill height of the solid support, the wall surface of the container is made of a breathable material or structure (mesh shape) It is necessary to increase the concentration of dissolved oxygen in the water to be added, or to exchange the water to be added at a high frequency to make gas exchange.

In a specific embodiment, when filling the solid carrier into the container in consideration of the above, the solid carrier of 0.1 to 100000 ml, preferably 1 to 10000 ml, can be filled. Further, as the filling height, the solid carrier can be packed so as to be a layer of 0.1 to 100 cm.

In the above filling step, a step of adding a microorganism group for carrying out the simultaneous doubling mineralization reaction in which the solid carrier is filled in the container and then the inorganic matter is made mineralized to produce nitrate nitrogen is added (microorganism group addition step).

As described above, when the solid carrier is stacked without being filled in the container, or when the solid carrier is integrally formed so as to maintain the solid shape as the solid carrier, it is possible to directly carry out the above- It is also possible to carry out the addition process.

In the present invention, the term " parallel doubly-charged inorganic reaction " means a reaction in which nitrite nitrogen is produced by mineralizing organic matter, and nitrification (nitrification) from ammonia to nitrogen nitrate ) Are continuously performed in the same reaction system.

Specifically, in the decomposition of organic matter, the organic nitrogen contained in the organic matter is decomposed into ammonia nitrogen, and the ammonia nitrogen is subjected to an oxidation reaction (nitrification reaction) by nitrification property to generate nitrate nitrogen will be.

In the present invention, nitrate nitrate generated by inorganicizing organic matter, nitrate ion or nitrate, specifically nitrate ion.

In the present invention, the group of microorganisms that perform the parallel doubly mineralization reaction includes a group of microorganisms that perform ammonia formation and a group of microorganisms that perform nitrification.

Examples of the "microorganism group that performs the simultaneous doubling mineralization reaction" that can be used in the present invention include a microorganism group that performs ammonia formation and a microorganism group that performs nitrification property, and a microorganism necessary for mineralizing the added organic matter to produce nitrate nitrogen Group.

Examples of the microorganism group constituting the microorganism group include ammonia-forming bacteria such as protozoa, bacterium and filamentous bacteria, and the like; Examples of the nitrifying bacteria (nitrifying bacteria) include Nitrosomonas genus, Nitrosococcus genus, Nitrosospira genus (including Nitrosolobus genus, Nitrosovibrio genus), and nitrite oxidizing bacteria (or nitrite producing genus) of ammonia oxidizing bacteria Genus Nitrospira; And the like.

Specific examples of the source of the microorganism to be added in the present step include a culture medium in which a microorganism group for performing a parallel double exposure reaction is cultivated, dried cells of the microorganism in which the culture solution is dried, a solid carrier on which the microorganism group is immobilized, A distillate obtained by adding water to the immobilized solid carrier and flowing out from the solid carrier; and a material derived from natural sources such as soil, tap water, lake water, water, well water, river water and seawater.

Particularly, it is preferable to add water to a culture medium in which a microorganism group for carrying out the simultaneous doubly-mineralization reaction is cultivated, a dried cell of the microorganism in which the culture liquid is dried, a solid carrier immobilized with the microorganism group, It is preferable that the effluent discharged from the carrier contains a sufficient amount of the microorganism group constituting the microbial ecosystem necessary for proceeding the parallel doubly-charged mineralization reaction.

In addition, as the 'solid carrier immobilizing the microorganism group', any of the solid carriers immobilized on the microorganism group may be used, but it is preferable to use those prepared through the process of the present invention.

The denitrification reaction is a phenomenon in which nitrate nitrogen is reduced by nitrous oxide gas or nitrogen gas or the like by denitrification bacteria and nitrate nitrogen is lost, and nitrate nitrogen which becomes an oxygen source and an organic component which is an energy source of denitrifying bacteria It is a reaction that is easy to induce when coexisting.

In the solid carrier of the present invention, organic matters are added before water is added to remove the nitrate nitrogen in the carrier, and when the conditions that easily cause the denitrification reaction such as the coexistence of the organic component and nitrate nitrogen at a high concentration , The denitrification reaction tends to occur when the pores in the solid support and / or the solid support are narrow, the system becomes a fresh water state due to capillary phenomenon, and the conditions for the denitrifying bacteria to become active due to the anaerobic tank in the solid support are established.

In the present invention, when the denitrification reaction occurs in the solid carrier, nitrate nitrogen which is excellent as an inorganic fertilizer component is gasified and lost, which is not preferable. Therefore, in the present invention, in the case where i) nitrate nitrogen is produced in a high concentration in a solid carrier, water is added and the solid carrier is washed out by removing water from the solid carrier to remove nitrate nitrogen, or ii) Or iii) the voids in the solid support and / or the solid support become too narrow as a whole, so that the capillary phenomenon becomes strong, and the solid support as a whole becomes an anaerobic state as fresh water It should be noted that the

The addition of the microorganism source to the container filled with the solid carrier is carried out by adding a culture of a microorganism group that performs the parallel multiplication mineralization reaction when the microorganism group is to be immobilized in a short time. In addition, the microorganism source may be added in the form of a powder or a state in which the microorganism source is in a liquid state.

The amount of the microorganism source to be added is not particularly limited. However, the amount of the microorganism source to be added is 1 to 1000 ml in the case of the culture medium, 1 to 1000 mg in the case of the dried cells, 1000 ml is preferably added.

In this process, by using nitrate nitrogen as the source of the microorganism (or by using nitrate nitrogen in the solid carrier charged in the previous step), nitrate nitrogen is contained in the solid carrier , It is preferable to wash the solid carrier by adding water after the addition of the microorganism source and flowing out from the solid carrier.

Here, the "case where nitrate nitrogen is contained in the solid carrier" refers to a case where nitrate nitrogen is included in a concentration and distribution in which denitrification occurs when coexisting with organic matter.

That is, in the case where nitrate nitrogen is not contained in the solid carrier in the concentration and distribution where the denitrification reaction is caused to occur when the organic carrier coexists with the organic carrier, the nitrate nitrogen is not contained in the solid carrier If not ".

Specific operations of the " cleaning " include: i) when the solid carrier is filled in a container having a drain hole, water is added and the drainage from the solid carrier is discharged from the drain hole .

When the solid carrier is filled in a container having no drain port, water is added and then the container is tilted to flow out the effluent from the solid carrier ).

In addition, when iii) the solid carrier is stacked without being filled in the container, or when the solid carrier is integrally molded so as to maintain the solid shape, water is added and then the solid carrier By directly draining the effluent of the reactor.

After the cleaning, the solid carrier is in a state in which nitrate nitrogen is not contained in a concentration and distribution in which a large amount of nitrate nitrogen is removed, that is, when denitrification occurs in the presence of organic matter. In addition, excess water is also spilled out.

As the water used for washing after the addition of the microorganism source, pure water (distilled water, ion-exchanged water, reverse osmosis membrane water, etc.), well water, river water, lake water, tap water, seawater and the like can be used. In addition, water containing a high concentration of nitrate nitrogen (specifically, 50 mg NO ₃ / ml or more) is not preferable.

The amount of water to be used for the washing is preferably an amount sufficient to rinse the entire solid carrier, and is preferably 100 to 10,000 ml per 1 L.

Since the nitrate nitrogen contained in the solid carrier can be lowered by removing the nitrate nitrogen by carrying out the cleaning, the conditions for causing the denitrification (the coexistence of the organic component and nitrate nitrogen at a high concentration) are not established , The denitrification reaction can be suppressed.

Therefore, when the organic substance is added to the solid support, the ammonia-forming reaction and the nitrifying reaction proceed without accumulation of the intermediate product, and the denitrification reaction does not occur.

Further, in the case where nitrate nitrogen is not contained in the concentration and distribution at which denitrification occurs when the organic carrier coexists with the solid carrier after the addition of the microorganism source, denitrification is carried out without washing the solid carrier Since it is in a state that it does not happen, it is not necessary to perform cleaning specifically.

After adding the microorganism source in the microorganism group addition process, when the solid carrier is washed by adding an organic substance and then adding water and flowing out from the solid carrier, nitrate nitrogen starts to be produced in the effluent (Immobilization step) of immobilizing the group of microorganisms performing the parallel doubly mineralizing reaction is carried out.

By the immobilization step, a desired solid carrier in which a group of microorganisms capable of carrying out a parallel double ionization reaction is immobilized can be produced.

The present step is a step for immobilizing the microorganism group on the solid carrier by fixing, purifying, and propagating.

In this immobilization step, the addition of the organic substance is performed by directly adding the organic substance to the solid carrier. Further, the organic material may be added in a state of being in a liquid state or in a state of powder.

Any organic material such as organic fertilizer, organic residue such as food residue, plant residue, animal wastes or feces can be used. However, the C / N ratio of the content ratio of carbon and nitrogen is 24 or less, preferably 19 Or less is preferable in view of improving the recovery efficiency of nitrate nitrogen.

As the organic substance, it is preferable to include a large amount of protein, proteolytic substance, amino acid, and the like.

Specifically, there can be mentioned food residues such as boiled broth, corn steep liquor, oil cake, fish meal, soybean meal, yeast meal, sake, soju meal, rice bran, and food waste. In addition, these are wastes obtained in the food manufacturing process, and are preferable in that they do not contain toxic components. In addition, animal fats can be used, and organic materials including ammonia nitrogen can also be used. Further, the food itself such as soybean meal, distilled spirit (amino acid scum), milk, and milk powder can be used. Furthermore, plant residues such as plant tissues or organs that can not be used as edible parts can be used.

Among them, it is more preferable to use boiled broth, fish meal, oil cake, food waste, corn steep liquor, rice bran, soybean meal, plant residue, milk, milk powder and livestock.

Specifically, broth that boiled bonito is an example of boiled broth. Examples of the corn steep liquor include corn steep liquor (CSL: a corn steep liquor which is a by-product of the production of cornstarch). Examples of the starch include vegetable seed oil, cone oil, and the like. Examples of the plant residue include foliage produced in the treatment of leaves during the cultivation management of tomatoes and the like. Examples of the food garbage include a seaweed of fish, vegetable debris after cooking, a piece of cut meat and the like. Examples of the livestock include cattle dung and cattle dung.

Further, it is particularly preferable because it is easy to be immersed in the solid carrier because the broth that boils bonito and the konseptirizo are liquid.

The amount of the organic substance to be added may be 0.01 to 20 g (on a dry weight basis), preferably 0.1 to 1 g (on a dry weight basis) relative to 1 L of the solid carrier.

More specifically, when the organic matter is in a liquid state, 0.1 to 20 g (weight of liquid: 0.07 to 14 g in terms of dry weight) is used when boiled broth is boiled, 20 g (liquid weight: 0.05 to 10 g in terms of dry weight)).

When the amount of the organic substance to be added is larger than the predetermined amount, the amount of the organic substance to be loaded on the solid carrier may be exceeded, which is not preferable. When the addition amount of the organic substance is less than the predetermined amount, it is not preferable because the concentration of nitrate nitrogen is lowered for the purpose of recovering nitrate nitrogen as a fertilizer component.

Then, after the addition of the organic matter, the microorganism group is " settled " to fix, purify, and propagate.

The temperature at which the solution is allowed to stand is 10 to 42 占 폚, preferably 15 to 37 占 폚, which is a temperature suitable for the growth of ammonia-forming and nitrifying microorganisms. When the temperature is lower than 10 占 폚, the proliferation of the microorganism is delayed, and it takes time for immobilization, which is not preferable. When the temperature is higher than 37 占 폚, a part of the microorganisms necessary for progressing the parallel doubly mineralization reaction may be killed, which is not preferable.

The period of "standing" in this step is a period during which the nitrate nitrogen is generated in the effluent when the solid carrier is washed by adding water and flowing out from the solid carrier, that is, Until the microorganism group is determined to be immobilized (by immobilization, purification and proliferation) on the solid carrier.

Specifically, the term " stationary " in the present step includes a culture solution in which a microorganism group for performing a parallel multiplication reaction is cultured, a dried cell of the microorganism obtained by drying the culture, a solid support on which the microorganism group is immobilized, (About 8 to 24 hours), preferably 3 days or more, more preferably 5 days or more, more preferably 5 days or more, when water is added to the solid carrier immobilized with the microorganism group and the effluent or the like flowing out from the solid carrier is used as a microorganism source. Day, and most preferably 7 days or more.

When the microorganism origin is derived from a natural source, the microorganism requires about 5 days or more, preferably 7 days or more, more preferably 2 weeks or more to complete the process.

In addition, when the period of time in this step is shorter than the period until nitrate nitrogen starts to be produced, the compliance of the group of microorganisms (nitrifying bacteria) that perform nitrification is not sufficient and the generation of ammonia nitrogen It is not desirable because it becomes a state.

Next, after standing as described above, the solid carrier is " washed " by adding water and flowing out from the solid carrier.

Specific operations of the " cleaning " include: i) when the solid carrier is filled in a container having a drain hole, water is added and the drainage from the solid carrier is discharged from the drain hole .

When the solid carrier is filled in a container having no drain port, water is added and then the container is tilted to flow out the effluent from the solid carrier ).

In addition, when iii) the solid carrier is stacked without being filled in the container, or when the solid carrier is integrally molded so as to maintain the solid shape, water is added and then the solid carrier By directly draining the effluent of the reactor.

After the cleaning is performed, extra water is also spilled out.

After the washing, the environment in the solid carrier is set to an aerobic state (it may be tightly mixed), which is preferable in order to suppress the propagation of microorganisms (denitrifying bacteria) that perform the denitrification reaction.

As the water to be used for washing after the settling, pure water (distilled water, ion exchanged water, reverse osmosis membrane water, etc.), well water, river water, water lakes, tap water, seawater and the like can be used. In addition, water containing a high concentration of nitrate nitrogen (50 mg NO ₃ / ml or more) is undesirable.

The amount of water to be used for the washing is preferably 100 to 3000 ml per 1 L of the solid carrier.

Since the nitrate nitrogen produced in the solid carrier can be removed and the concentration of nitrate nitrogen contained in the solid carrier can be lowered by carrying out the cleaning, the conditions for causing the denitrification (the organic component and the nitrate nitrogen at a high concentration Coexistence condition) is not established, and the denitrification reaction can be suppressed.

Therefore, when the organic material is added to the solid carrier, the ammonia-forming reaction and the nitrifying reaction proceed without accumulating reaction products, and the denitrification reaction can be suppressed.

The determination of whether the microorganism group has been immobilized (by fixing, purifying, or multiplying) on the solid carrier can be performed by measuring the concentration of nitrate nitrogen in the effluent at the time of the washing.

That is, when the concentration of nitrate nitrogen in the effluent is measured, it is judged that the microorganism group is immobilized on the solid carrier when it is confirmed that the organic matter is mineralized in the effluent and nitrate nitrogen is started to be produced can do.

Preferably, when the production of nitrate ions of not less than 50 mg NO ₃ / L, more preferably not less than 200 mg NO ₃ / L is confirmed in the effluent, the microorganisms are classified into 'sufficient 'Is fixed.

The determination that the microorganism group is immobilized on the solid carrier in this manner leads to a remarkable improvement in the reaction rate (in particular, the nitrification ability) of the parallel double mineralization reaction in the solid carrier. As a result, the added organic matter is rapidly decomposed, and at the point in time when nitrate nitrogen starts to be produced, the organic component which is the energy source of the denitrifying bacteria (denitrifying bacteria) is lost, It becomes an environment where the denitrification reaction is difficult to occur.

Further, when it is not confirmed that the microorganism group is immobilized (or "sufficiently immobilized") on the solid carrier, it is preferable that "the organic substance is added, then the mixture is allowed to stand, and water is added to the solid carrier to flow out from the solid carrier, Solid carrier " is repeatedly performed until the above-mentioned criterion is satisfied.

That is, when nitrate nitrogen is not started to be produced in the effluent flowing out when the solid carrier is washed, the treatment is repeatedly carried out until nitrate nitrogen is produced in the effluent flowing out during the washing .

As described above, in the present immobilization step, when the microorganism source is added in the step of adding a microorganism, (1) 'the organic substance is added, and then water is added and the solid carrier is washed out by flowing out from the solid carrier (2) 'when the microorganism group is not confirmed to be immobilized on the solid carrier, (2) the organic substance is added to the solid carrier, and The solid carrier is washed by adding water and flowing out from the solid carrier by repeating " repeatedly " until nitrogen nitrate is produced in the effluent flowing out during the washing .

That is, this immobilization step can be performed by combining the operations of (1) and (2).

Further, in the operation (1), when the settling time is taken as long as the predetermined period described above, it is possible to complete the process only by performing the cleaning once after the addition of the organic material.

In order to monitor the production status of nitrate nitrogen contained in the effluent flowing out during the washing in this step, preferably, in order to check whether or not the microorganism group in the solid carrier is immobilized, 2 ") may be performed.

More specifically, in the case of performing the " repetition " operation as in (2), the production of nitrate nitrogen can be grasped in detail by repeating the operation once every 1 to 7 days, and more preferably, every day.

In addition, in order to suppress the propagation of the denitrification-causing microorganisms (denitrifying bacteria), it is desirable that the number of repetition operations of (2) is as few as possible. Repetition "operation as in the case (2) is frequently performed, the number of times of cleaning increases, so that the amount of water in the solid carrier becomes large, and the anaerobic condition tends to be established. Therefore, the activity of the group of microorganisms (nitrifying bacteria) that perform nitrification is suppressed, and the breeding of a group of microorganisms (denitrifying bacteria) that perform the denitrification reaction tends to occur.

The period of time required to complete all of the above processes, that is, the period of time required for confirming that the microorganism group has been immobilized or "sufficiently" immobilized on the solid carrier, can be determined by culturing a microorganism group When a microorganism is used as a microorganism source, a dried cell of the microorganism obtained by drying the culture liquid, a solid carrier immobilized with the microorganism group, a effluent leaking from the solid carrier by adding water to the solid carrier immobilized with the microorganism group , Overnight (about 8 to 24 hours), preferably 3 days or more, more preferably 5 days or more, and most preferably 7 days or more.

When the microorganism origin is derived from a natural source, the microorganism requires about 5 days or more, preferably 7 days or more, more preferably 2 weeks or more to complete the process.

In this way, a solid carrier immobilized with a group of microorganisms that undergo parallel doubly mineralization reaction can be produced.

By carrying out the above steps, the solid carrier immobilized (by fixing, purifying, and propagating) the microorganism group that performs the parallel doubly mineralization reaction is a carrier of a catalyst column for producing nitrate nitrogen, which is an inorganic fertilizer component from organic matter Can be used.

Further, in the case where the above-described process is performed using the container having the drain hole, it can be used as a catalyst column in a state of being filled in the container provided with the drain hole.

It is also possible to use a solid carrier in which the microorganism group is immobilized in a new separate container.

In addition, even in the case where the filling step is not carried out by using the solid carrier integrally molded so as to maintain the solid shape, the solid carrier on which the obtained microorganism group is immobilized can be cut and ground as required, To be used as a catalyst column.

As the catalyst column of the present invention, when the organic matter to be decomposed is 1 g per day, 100 ml or more of the catalyst column may be charged with the solid carrier immobilized thereon.

Further, in the catalyst column of the present invention, the solid support is packed such that a part of the pores in the support or the support is larger than a certain size so as to ensure both the air permeability and the water retention so that the solid support functions as a column support.

That is, the height h of the liquid surface due to the capillary phenomenon (in units m) (calculated by the formula I) does not exceed the filling height of the solid carrier (the inner diameter (radius) (m ) R) is secured at a portion thereof. Further, at the time of charging, the solid carrier may be charged so as to be uniform, but it is more preferable to charge the solid carrier to be compact.

In addition, when both the pores in the carrier or in the carrier are small, and therefore the rising height (h) of the liquid surface due to the capillary phenomenon exceeds the filling height of the carrier, when the water is added to the catalyst column, Which is anaerobic, and the denitrification reaction tends to be caused, which is not preferable.

By using the catalyst column of the present invention, a fertilizer containing nitrate nitrogen, which is an inorganic fertilizer component produced from organic matter, can be obtained.

As the fertilizer containing the nitrate nitrogen as the inorganic fertilizer component, the nitrate ion of 50 mg NO ₃ / L or more, preferably 200 mg NO ₃ / L or more is contained in the effluent from the catalyst column .

Further, when the catalyst column of the present invention is used, the amount of nitrate nitrogen as an inorganic fertilizer component per day from the organic matter is about 270 mg NO ₃ or more, about 60 mg in terms of nitrogen, N or more nitrate nitrogen is produced.

In addition, even when the solid carrier integrally molded so as to maintain the solid shape in which the microorganisms are immobilized is not filled in the container, water is directly added to the solid carrier to recover the effluent flowing out from the solid carrier , It is possible to obtain a fertilizer containing nitrate nitrogen which is an inorganic fertilizer component.

The amount of the organic substance to be added when preparing the fertilizer containing nitrate nitrogen as the inorganic fertilizer component is 0.01 to 20 g (1 L) per 1 L of the solid carrier on which the microorganism is immobilized Dry weight conversion), preferably 0.1 to 1 g (on a dry weight basis) may be added. Specifically, when the organic matter is in a liquid state, 0.1 to 20 g (liquid weight: 0.07 to 14 g in terms of dry weight) is used when boiled broth is boiled, and 0.1 to 20 g g (liquid weight: 0.05 to 10 g in terms of dry weight)).

Further, when the addition amount of the organic matter is larger than the predetermined amount, the nitrifying reaction can not be followed and the ammonia nitrogen concentration of the effluent is increased, which is not preferable. However, there is no problem when it is intended to produce a fertilizer having a high ammonia content (for example, fertilizer for soil cultivation).

The fertilizer containing nitrate nitrogen, which is an inorganic fertilizer component, can be used as a fertilizer for cultivation of all kinds of plants such as vegetables, fruits, flowers, trees, and plants.

Especially, leafy vegetables such as Cheonggyeongjae, Komatsunaen, lettuce, spinach, etc .; Tomatoes such as tomatoes for harvesting fruit; It can be preferably used for the cultivation of fruit trees. Especially, it can be suitably used for the cultivation of cheongcheongchae, filigree, which is a leaf vegetable.

The fertilizer containing nitrate nitrogen, which is the inorganic fertilizer component, can also be used for the cultivation of plants which are generally carried out, such as nutrient solution cultivation in hydroponics, nutrient solution cultivation in solid tubers, and cultivation using ordinary soil .

As described above, the catalyst column of the present invention can remarkably improve the reaction rate of the simultaneous double mineralization reaction, and can efficiently produce nitrate nitrogen as an inorganic fertilizer component from organic matter.

This is because a high catalyst surface area and high aerobic reaction conditions can be ensured unlike the simultaneous doubly mineralization reaction in the liquid state in the conventional method. That is, by using a solid carrier immobilized with a high density of microorganisms, the surface area of catalyzing the reaction becomes large, and water retention is ensured in the narrow pores of the carrier (due to capillary phenomenon: constant water retention is required for the growth of microorganisms) This is because the microorganisms that catalyze the simultaneous double mineralization reaction can be activated and the denitrification reaction can be suppressed by ensuring the air permeability (exhalation reaction condition).

Further, by using the catalyst column of the present invention, it is possible to produce a fertilizer containing nitrate nitrogen, which is an inorganic fertilizer component, from an organic matter without performing operation using aeration or constant power (by energy saving method) .

Particularly, it is advantageous when a large-scale production is carried out. Thus, in the treatment of organic wastes, which have been costly but have not produced valuable ones so far, the organic fertilizer containing organic fertilizer and organic fertilizer as raw materials, while suppressing operation cost and installation cost at low cost, It is possible to produce, manufacture and sell fertilizers containing nitrate nitrogen. This is a breakthrough in the waste treatment industry, which has not been profitable.

In the present invention, by carrying out the above-described steps, the solid carrier immobilized by the microorganism group that performs the parallel doubly mineralization reaction can be directly added with the organic fertilizer to perform the nutrient solution cultivation Quot; plant-grown solid medium ".

That is, by using the plant-cultured solid medium, it is possible to directly add the organic fertilizer even in the case of a solid germination scale to conduct the nutrient solution cultivation.

Further, the nutrient solution cultivation in the solid gypsum can be performed by repeating the operation of adding the organic fertilizer directly to the solid medium after irrigation.

The addition of the organic fertilizer is carried out by directly adding to the plant-grown solid medium. In addition, the fertilizer containing the organic matter may be added in a liquid state or in a powder state.

As the organic fertilizer which can be directly added and used in the case of culturing the plant-grown solid medium using the plant-cultivated solid medium, the above-described organic fertilizer can be used as fertilizer. Preferably, the organic fertilizer , That is, a broth or a corn steep liquor, or a solid or organic material, may be used.

The addition of the organic fertilizer is performed by directly adding the organic fertilizer to the plant-cultured solid medium. In addition, the fertilizer containing the organic matter may be added in a liquid state or in a powder state.

As the added amount of the organic fertilizer, 0.01 to 20 g (on a dry weight basis), preferably 0.1 to 1 g (on a dry weight basis) may be added to 1 L of the solid carrier on which the microorganism is immobilized. Specifically, when the organic fertilizer is in a liquid state, 0.1 to 20 g (liquid weight: 0.07 to 14 g in terms of dry weight) is used when boiled broth is boiled, and 0.1 to 20 g in terms of cone- To 20 g (liquid weight: 0.05 to 10 g in terms of dry weight)).

Further, when the addition amount of the organic fertilizer is more than the predetermined amount, the nitrifying reaction can not be followed and the ammonia nitrogen concentration of the effluent increases, which is not preferable. However, there is no problem as long as it does not cause a growth disorder at the time of cultivation.

When the solid carrier on which the microorganism group is immobilized is used as the plant-cultured solid medium, the solid carrier may be any of the solid carrier materials described above. Preferably, the solid carrier is selected from the group consisting of rock wool, vermiculite, perlite, , Urethane, and more preferably a rock surface.

The nutrient solution cultivation in which the fertilizer containing the organic substance is directly added using the plant-cultivated solid medium can be used for the cultivation of all plants such as vegetables, fruits, flowers, trees, fruit trees, and plants.

Especially, the cultivation of the plant, the leafy vegetables such as the cheongcheongchae, the suit, the lettuce, the herb, etc .; tomatoes, eggplant, bell pepper, melon, watermelon, strawberry etc. It can be suitably used for the cultivation of fruit trees. In addition, it can be particularly preferably used for the cultivation of cheongcheongchae and filigree, which are leafy vegetables.

Further, in the present invention, the solid carrier immobilized by the microorganism group performing the parallel doubly mineralization reaction obtained by the above-mentioned steps may be used as a microorganism material (microorganism source) which catalyzes the double- As shown in Fig.

Concretely, it can be used as a 'microbial source' of a microorganism group to be added in the charging step of the present invention and to be subjected to the simultaneous double-walled mineralization reaction. Furthermore, it can be used as a seed bacterium of a microorganism group optimized for 'simultaneous doubly mineralization reaction' in water.

Example

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

Example 1 (Examination of material of solid carrier 1)

(Manufactured by Wako Pure Chemical Industries, Ltd.)) and various solid carriers (manufactured by Grodan), vermiculite (manufactured by BISELITE Co., Ltd.), pearlite (manufactured by Komari Co., Ltd.), Kanumato It was examined whether or not a microorganism group for performing a doubly-mineralizing reaction could be immobilized and inorganic matter could be made mineralized to produce nitrate nitrogen.

First, 10 L of water was put into a Wagner pot (manufactured by Fujiwara Seisakusho), and 80 g of bark compost (Kiyomizu Kogyo Kogyo Cooperative), 8 g of boiled broth (a by-product of a bonito fish plant, ) (Hereinafter, the same) was added and aerated with an air pump to cultivate a group of microorganisms that were subjected to the simultaneous double exposure reaction.

Next, the bottom of the PET bottle for 500 ml was cut to obtain the upper half side, and the upper and lower sides were reversed while the lid was opened. A container in which the lid of the PET bottle became a drainage port was prepared, 100 ml of various solid supports were filled. 4 is a photographic top view showing a state in which various solid carriers are filled in a container provided with a drain port;

Next, 200 ml of the culture solution in which the microorganism group performing the parallel multiplication reaction was cultivated was inoculated as a microorganism source, 100 ml of distilled pure water was added, and the various solid carriers were washed out by draining from the drainage port.

Thereafter, 0.1 g (liquid weight) of boiled broth of boiled tuna was added as an organic substance, and the mixture was allowed to stand overnight at room temperature (about 25 DEG C) and then washed with 100 ml of distilled water until the number of days shown in Fig. 5 And repeated. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

The nitrate ion concentration was measured by measuring with RQ-flex (manufactured by Merck Co.) using Reflectoquant nitric acid test paper (manufactured by Merck) (hereinafter, nitrate ion concentration was measured in the same manner in this example). ).

In addition, as a control experiment (control), the microorganism source was not added to the container filled with the above various solid carriers. The results are shown in Fig.

As a result, the production of nitrate ions from the effluent was confirmed for any of the above various solid carriers. That is, it has been shown that the microorganism group is immobilized to any of the above-mentioned various solid carriers and catalyzes the parallel double ionization reaction. Further, in the control experiment in which the microbial source was not added, generation of nitrate ions was not confirmed.

In addition, all of these various solid carriers are made of a porous material having air permeability.

Further, in detail with respect to the results shown in Fig. 5, it was shown that nitric acid ions were produced in the effluent after 2 to 12 days from the addition of the microorganism source, at a concentration of 30 mg / L or more.

When the various solid carriers other than the Nanumato, that is, the urethane, the rocky surface, the pearlite and the vermiculite are used, the effluent after 6 to 7 days from the addition of the microorganism source is added to the effluent of about 50 mg / L or more, Nitric acid ions of not less than 70 mg / L were produced, and nitric acid ions of about 200 mg / L or more were produced particularly when urethane or rockface was used.

These results indicate that these materials are materials that are particularly well-ventilated and denitrification-prone to occur, and are capable of immobilizing a large number of microorganisms having a large surface area per volume, .

Therefore, it has been shown that even when any of the various solid supports is used, a catalyst column that produces nitrate nitrogen, which is an inorganic fertilizer component, can be produced from the added organic matter.

Example 2 (inoculation of dried cells)

It was verified that the microorganism was able to be used as a dried cell body (not a liquid) as a microbial source of a microorganism group that undergoes simultaneous doubly mineralization reaction.

The supernatant of the culture solution after the simultaneous doubly fortified mineralization reaction described in Example 1 was discarded and the biofilm (microbial community structure) formed on the wall was air-dried to obtain dried microbial cells.

Next, the bottom of the PET bottles for 500 ml is cut to obtain the upper half, and the upper and lower sides are reversed while the lid is opened. A container in which the lid of the PET bottle is a drain hole is prepared. 100 ml).

10 mg of the dried microbial cells of the microorganism group was added as a microorganism source, inoculated, 100 ml of distilled water was added, and the granular surface was washed out from the drainage port.

Thereafter, 0.1 g (liquid weight) of boiled broth was added as an organic substance, and the mixture was allowed to stand overnight at 37 占 폚 and then washed with distilled pure water (100 ml) until two days elapsed.

Thereafter, the mixture was allowed to stand at 37 캜 until 22 days had elapsed (24 days after the addition of the microorganism source was added) without adding the boiled broth and washing treatment, and 100 ml of distilled pure water . At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured. The results are shown in Fig.

As a result, it was shown that the microorganism group was immobilized on the rock-wedge-shaped granular surface even when the microorganism was inoculated using a dry cell-shaped material, thereby catalyzing the simultaneous double mineralization reaction.

From these results, it was found that the microorganism source to be inoculated may be in the form of dried cells.

Example 3 (Examination of material of solid carrier 2)

In addition to the above solid carrier materials, in addition, 15 g of solid carrier materials (5 g of rice straw (manufactured by Fujiwara Chemical Co., Ltd.), 2 g of impregnated material (manufactured by Go Merry Co., Ltd.), 10 g of cedar chips , 20 g of bamboo charcoal (manufactured by Sejudo Co., Ltd.), 10 g of cotton wool (manufactured by Isen Kogyo Co., Ltd.), 10 g of filter paper (Advantec Co., Ltd.), 1.25 g of polyacrylamide gel 100 g of 1.6% agar (manufactured by Wako Pure Chemical Industries, Ltd.), 50 g of zeolite (manufactured by Takamura), 50 g of sand (manufactured by Sudo Co., Ltd.), 50 g of ceramics A microorganism group for performing a parallel doubly mineralization reaction was immobilized to 60 g of bead (manufactured by Sudo Co., Ltd.), 6.3 g of nylon (manufactured by Go Merry Co., Ltd.) and 1 g of melamine resin (manufactured by Go Merry Co., And it was examined whether or not it could generate nitrogen gas.

The bottom of a PET bottle for 500 ml was cut to obtain the upper half portion and the upper and lower sides were reversed with the lid opened to prepare a container in which the lid of the PET bottle became a drainage port. (An amount corresponding to 20 to 100 ml).

To this was added 10 mg of dried cells of the microorganism group for carrying out the parallel doubly-charged mineralization reaction used in Example 2 as a source of microorganism, inoculated, 100 ml of distilled pure water was added, and the mixture was discharged from the drainage port, .

Thereafter, as the organic matter, 0.1 g (liquid weight) of boiled broth was added, the mixture was allowed to stand overnight at 37 占 폚, and then washed with 100 ml of distilled pure water until the number of days shown in Fig. 7 or 8 elapsed Respectively. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

As a result, it was confirmed that nitrate ions were generated from the effluent in any of the above 15 kinds of solid carriers. That is, it has been shown that the microorganism group is immobilized to any of the above-mentioned various solid carriers and catalyzes the parallel double ionization reaction.

Among them, nitric acid ions of about 40 mg / L or more are produced in the effluent after 8 days from the addition of the microorganism source, particularly when nylon, fertilized, fertilizer paper, cedar chips, sand, ceramics and zeolite are used , And nitrate ions of about 50 mg / L or more (especially about 60 mg / L or more) were produced when nylon, conception, fertilizer paper or cedar chips were used.

From these results, it has become clear that organic materials such as natural resins, chemical resins, minerals such as glass and sand, ceramics and the like can be used as the material of the solid carrier.

Example 4 (Examination of kinds of various organic substances 1)

Whether or not the organic matter could be made into a mineral by simultaneous double mineralization reaction and nitrate nitrogen could be produced even when an organic material other than the boiled broth was used as the organic material in Example 1 was examined.

500 ml bottles were cut to obtain the upper half of the flask, and the upper bottle and the lower bottle were inverted with the lid opened. A container in which the lid of the PET bottle was drained was prepared, and 70 g of vermiculite (250 ml in volume) .

To this was added 500 ml of a culture solution obtained by culturing a microorganism group for carrying out the parallel doubly mineralization reaction used in Example 1 as a microorganism source, inoculated, and 250 ml of distilled pure water was added thereto to flow out from the drainage port to wash the vermiculite.

Thereafter, 0.2 g of seed oil or 0.2 g (liquid weight) of corn starch (CSL: corn steep liquor as a by-product of the production of corn starch) was added as an organic substance and allowed to stand overnight at room temperature (about 25 캜) The treatment of washing with 250 ml of pure water was repeated until the number of days shown in Fig. 9 or 10 elapsed, respectively. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

In addition, as the control experiment (control), the microorganism source was not added to the vessel filled with the above-mentioned vermiculite. Fig. 9 shows the result of adding seed oil, and Fig. 10 shows the result of adding CSL.

As a result, nitrate ion production was confirmed from the effluent even when any of organic matter, oilseed oil or CSL was used. That is, it has been shown that the microorganism group is immobilized on the vermiculite to catalyze a parallel doubly mineralization reaction. Further, in the control experiment in which the microbial source was not added, generation of nitrate ions was not confirmed.

In particular, when the addition of the CSL-containing liquid was continued, it was shown that a stable concentration of nitrate ions was produced every day after 6 days from the addition of the microorganism source. The conversion efficiency of the organic nitrogen contained in the CSL to the nitric acid precursor was 98.6%, which is very high conversion efficiency.

Therefore, it has been shown that, when CSL is added as an organic substance, the effluent containing nitrate nitrogen at a high concentration can be recovered.

Example 5 (Examination of kinds of organic substances 2)

It has also been investigated whether organic substances other than the above-mentioned organic substances can be used to produce nitrate nitrogen by inorganic enrichment reaction.

500 ml bottles were cut to obtain the upper half of the bottle, and a container with a lid of a plastic bottle as a drainage port was prepared by reversing the upper and lower sides with the lid opened. 10 g (volume 50 ml) Lt; / RTI >

To this was added 10 mg of dried cells of the microorganism group for carrying out the parallel doubly-charged mineralization reaction used in Example 2 as a source of microorganism, inoculated, 100 ml of distilled water was added, and the granular surface was washed out by draining from the drain.

Then 0.1 g of food residue (food waste, nitrogen content 6%) was added as an organic substance, and the mixture was allowed to stand overnight at 37 占 폚 and then washed with 100 ml of distilled pure water Was repeated until the number of days shown in Fig. 11 elapsed. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured. The results are shown in Fig.

As a result, even when any of organic matter, fish meal (Doosho), cone oil (Doosho), and plant residue was used, the production of nitrate ions was confirmed from the effluent, and the effluent containing nitrate nitrogen could be recovered .

Example 6 (Examination of kinds of organic substances 3)

It has also been investigated whether organic substances other than the above-mentioned organic substances can be used to produce nitrate nitrogen by inorganic enrichment reaction.

500 ml bottles were cut to obtain the upper half of the bottle, and a container with a lid of a plastic bottle as a drainage port was prepared by reversing the upper and lower sides with the lid opened. 10 g (volume 50 ml) Lt; / RTI >

To this was added 10 mg of dried cells of the microorganism group for carrying out the parallel doubly-charged mineralization reaction used in Example 2 as a source of microorganism, inoculated, 100 ml of distilled water was added, and the granular surface was washed out by draining from the drain.

Thereafter, as organic substances, there were used organic substances such as rice bran (made by Tsukemoto Co., Ltd.), powdered milk (manufactured by Morinaga Kogyo Co., Ltd.), distilled spirits (Shimaya Co., Ltd.), soy sauce (made by Safe Tech International) 0.1 g (liquid weight in the case of milk) was added to the mixture at room temperature (about 25 占 폚), overnight at room temperature (about 25 占 폚) After that, the process of washing with pure distilled water (100 ml) was repeated until the number of days shown in Fig. 12 elapsed. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

As a result, even when any of the above organic materials was used, the production of nitrate ions was confirmed from the effluent, and it was shown that the effluent containing nitrate nitrogen could be recovered.

Of these, rice bran has high C / N ratio (content ratio of nitrogen to carbon) of 18.1, which is an organic resource that can not recover nitrate nitrogen in the conventional simultaneous double mineralization reaction in water, It was made clear that nitrate nitrogen could be formed by the addition of nitric acid by adding nitric acid.

This is considered to be the main reason why the reaction rate in the solid support was doubled as compared with the conventional double reaction in the presence of water. That is, it is considered that the solid carrier has a high air permeability and thus an aerobic condition is always maintained, and a microorganism group that catalyzes the reaction has a large surface area on which immobilized nitric acid groups are immobilized, thereby significantly accelerating the decomposition nitrification rate of organic components . Further, it is considered that ammonia is easily diffused into moisture contained in the carrier, while the diffusion of the organic component is difficult to occur in the solid carrier, and a region where the C / N ratio is lowered microscopically occurs.

Example 7 (Use of microorganism source derived from nature)

It was examined whether or not a microorganism group derived from the natural world contained in soil, seawater, tap water or the like could be used as an inoculation source of a microorganism immobilized on a solid carrier.

500 ml bottles were cut to obtain the upper half of the bottle, and a container with a lid of a plastic bottle as a drainage port was prepared by reversing the upper and lower sides with the lid opened. 10 g (volume 50 ml) Lt; / RTI >

100 ml of seawater, 100 ml of tap water, or 1 g of soil was added as a microorganism source, inoculated, 100 ml of distilled water was added, and the granular surface was washed out from the drain hole by washing.

Thereafter, as the organic matter, 100 mg of boiled broth was added, and the mixture was allowed to stand overnight at room temperature (about 25 캜) and then washed with 100 ml of distilled water. When the number of days shown in each of Figs. 13 or 14 . At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

Further, the distilled water was added to the container filled with the above-mentioned rock-face-side granular surface in place of the microorganism source, and this was used as a control experiment. Fig. 13 shows the result of inoculating seawater or tap water as a microorganism source, and Fig. 14 shows the result of inoculating the soil as a microorganism source.

As a result, nitrate ion production was confirmed from the effluent even when any of seawater, tap water, and soil was inoculated as a microorganism source. That is, it has been shown that the microorganism group derived from nature is immobilized on the rock face granular surface to catalyze the parallel doubly mineralization reaction. Further, in the control experiment in which pure water of distillation was added in place of the microorganism source, generation of nitrate ions was not confirmed.

From these results, it has been found that microorganisms capable of carrying out the parallel doubly mineralization reaction can be immobilized on a solid support by using a naturally occurring microorganism group contained in soil, seawater or tap water as an inoculation source.

However, it has been shown that immobilization of microorganisms contained in seawater or tap water is time-consuming as compared with the case of using the culture solution of the microorganism group prepared in Example 1. When the soil was inoculated, it was found that immobilization was possible relatively quickly.

Example 8 (Use of column effluent as a microbial source)

An effluent from a solid carrier on which a microorganism group that already catalyzes the co-multiplication reaction is immobilized (a effluent from a catalyst column packed with a solid carrier on which the microorganism group is immobilized) is immobilized on a separate solid carrier Experiments were conducted.

The bottom of the PET bottles for 500 ml was cut to obtain the upper half of the bottle. When the bottle was opened and the upper and lower sides were reversed, a container in which the lid of the PET bottle was drained was prepared. 20 g (volume 100 ml) Lt; / RTI >

100 ml of the effluent from the catalyst column packed with the rock-wool granular surface described in Example 1 was added as a microorganism source, inoculated, 100 ml of distilled water was added, and the granular surface was washed out by draining from the drain.

Thereafter, 0.1 g (liquid weight) of boiled broth of boiled tuna was added as an organic substance, and the mixture was allowed to stand overnight at room temperature (about 25 캜) and then washed with 100 ml of distilled water until the number of days shown in Fig. 15 And repeated. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

Further, as a control experiment (control), the microorganism source was not added to the container filled with the above-mentioned dark-side surface granular surface. The results are shown in Fig.

As a result, in the case of adding the "effluent from the catalyst column" filled with the rocky granular surface immobilized with the microorganism group described in Example 1 as a source of microorganisms, the concentration of nitrate ions produced in the effluent from the drainage port, After about 7 days, it showed a high value of about 220 mg / L. In the same manner as in the case of using the culture solution shown in Examples 1 and 2 as a microorganism source, the microorganism group .

Further, in the control experiment in which the microbial source was not added, generation of nitrate ions was not confirmed.

From these experimental results, it was found that it is possible to use the effluent from the catalyst column using the already solidified support as a microorganism source of the separate solid carrier.

Example 9 (Examination of immobilization in a dancer 1)

It was examined whether or not the above-mentioned microorganism group could be directly immobilized on a solid carrier integrally molded so as to maintain a solid shape, without using the column method (a method of filling a container having a drain hole into a solid carrier).

First, the cultivation medium was cultured in a hydroponic culture by using a culture solution after the simultaneous doubling mineralization reaction described in Example 1, and after cultivation, a culture broth of the microorganism not containing nitrate nitrogen was obtained.

Next, 500 ml of distilled pure water was added directly to a rocky face cube (manufactured by Grodan, 100 × 100 × 100 mm, volume: 1 L) integrally molded so as to maintain the solid shape, and excess water was flowed out from the rocky face cube, Respectively.

To this, 500 ml of the nitrate-nitrogen-free culture solution was added as a microorganism source and inoculated. Further, after addition of the microbial source, washing of the rocky face cube with distilled pure water was not performed.

Thereafter, 0.5 g of boiled broth was added as an organic substance, and the mixture was allowed to stand for 6 days at room temperature (about 25 ° C), and then washed with distilled pure water (500 ml). The liquid flowing out from the rocky cube was recovered and nitrate ion concentration was measured. The results are shown in Fig.

As a result, even when a rock surface (solid carrier) integrally molded so as to maintain a solid shape is used without using the column method (a method of filling a solid carrier with a drain port), the addition of the microorganism source Day, nitrate ions of about 50 mg / L were produced.

That is, when there is a porous structure carrying microorganisms, organic substances, and water in the solid carrier itself, it has been shown that the solid carrier can be integrally formed so as to maintain the solid shape, so that the microorganism can be immobilized without being charged into the vessel.

Further, it has been shown that when a microorganism source containing no nitrate ion is used, the microorganism group is immobilized on the solid carrier and the parallel doubly-charged mineralization reaction is catalyzed without washing the solid carrier (rockwool cube) with water after inoculation. Thus, when a microorganism source containing no nitrate ions was used, it was found that the operation of washing with water after addition of the microorganism source could be omitted.

In this case, it is not necessary to repeat the steps of setting and washing with water after the addition of the organic substance, and it is also shown that microorganisms can be purified and proliferated only by adding an organic substance and standing for several days, lost.

This result is considered to be because the nitrate nitrogen concentration in the solid carrier is low at the time of adding the organic matter, and the denitrification reaction is hardly caused.

Example 10 (Examination of immobilization in a dancer 2)

A solid carrier integrally molded so as to maintain a solid shape without using the column method (a method of filling a container having a drain hole into the solid carrier) was further investigated.

First, a rocky face cube (100 x 100 x 100 mm, volume: 1 L), a cotton (20 g, volume: 100 mL, manufactured by Isen Kogyo Co., Ltd.) and a melamine resin , 2 g: volume 50 mL) was prepared. In addition, the cotton and the melamine resin were put in a state of being tucked (see Fig. 17).

To this was added 10 mg of the dried cells of the microorganism group which was used in Example 2 to carry out the parallel doubly mineralization reaction as a microorganism source and inoculated. Further, in this embodiment, since the added amount of the microorganism source itself was very small (that is, the amount of nitrate nitrogen contained in the microorganism source was also very small), after the addition of the microorganism source, The solid carrier was not washed.

Thereafter, as an organic substance, 0.1 g of boiled broth was added, and the mixture was allowed to stand overnight at 37 캜 and then washed with distilled pure water (100 ml) until the number of days shown in Fig. 18 elapsed. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured. The results are shown in Fig.

As a result, it was shown that the production of nitrate ions was confirmed 6 days after the addition of the microorganism source, even when a cotton or melamine resin other than the rock surface was integrally molded so as to maintain the solid shape.

That is, it has been shown that if the solid carrier such as a resin is integrally molded so that the solid shape is maintained without filling the solid carrier in the container, it is possible to immobilize the microorganism group without any problem.

When the rock surface was used, nitrate ions at a concentration of about 240 mg / L were produced in the effluent after 8 days from the addition of the microorganism source. Particularly, as a material of the solid support for immobilizing the microorganism group, Preferred ones have been shown. This is considered to be because the rock surface is a material excellent in terms of air permeability and surface area where the microorganism group is immobilized.

Example 11 (Fixation of microorganism group by decantation)

In addition to the above column method (a method of filling a solid carrier with a container having a drain hole), it was examined whether the microorganism group could be immobilized on a solid carrier even when cleaning was carried out by decantation.

A plastic cup (200 ml capacity) was charged with 10 g (volume 50 ml) of the rock surface. To this was added 10 mg of the dried cells of the microorganism group which was used in Example 2 to carry out the parallel doubly mineralization reaction as a microorganism source and inoculated. Further, in this embodiment, since the amount of the added microorganism source itself was very small (that is, the amount of nitrate nitrogen contained in the microorganism source was very small), after the addition of the microorganism source, The solid carrier was not washed.

Thereafter, 0.1 g of boiled soup broth was added as an organic substance, and the mixture was allowed to stand at 37 占 폚 overnight, 100 ml of distilled water was added, and the mixture was allowed to stand for several minutes. Thereafter, Washing treatment by means of a washing machine) was repeated until the number of days shown in Fig. 19 elapsed. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

As a result, even when cleaning was carried out by decantation without using the above-mentioned column method (a method of filling a solid carrier with a drainage port), about 40 mg / L was taken 6 days after the addition of the microorganism source, It was shown that after 8 days about 75 mg / L nitrate ion was produced.

From this, it has been shown that the microorganisms can be immobilized on the solid carrier by washing with decantation even if they are not charged in the column (container having a drain hole).

Example 12 (Nutrient solution cultivation in which organic fertilizer was directly added to a solid base)

It has been examined whether a nutrient solution can be cultivated by directly adding a fertilizer containing an organic matter in a solid size range by using a solid support immobilized with a group of microorganisms performing a parallel doubly mineralizing reaction as a solid medium for plant growth.

500 ml of a culture solution obtained by culturing a microorganism group for carrying out the parallel doubly-charged mineralization reaction as used in Example 1 on a rock-bottom cube (solid carrier of Grodan Co., 100 × 100 × 100 mm: volume 1 L, 500 ml of tap water was added thereto, and water was drained from the rock surface cube to wash the rock surface cube. Further, 20 containers filled with this rock face were defined as one compartment.

Thereafter, 0.5 g (liquid weight) of boiled broth was added to each rocky cube as a liquid containing organic matter, and the mixture was allowed to stand overnight at room temperature (about 25 DEG C), washed with 500 ml of tap water Was repeated until 7 days passed from the inoculation of the microorganism group.

Next, after confirming that nitrate ions were produced in the effluent after 8 days from the inoculation of the microorganism group, the seeds were seeded in a rocky cube. After the seeding, the addition of the boiled broth and the washing treatment were stopped. Cultivation was conducted in a greenhouse (without heating) from December 6, 2007 to January 9, 2008.

After 10 days from sowing (after 18 days from the addition of the microorganism source), the operation of repeating the addition of the boiled soup and the washing treatment from the stage where the cotyledon was developed was resumed, and until the 34 days elapsed from sowing (Until 42 days had elapsed from the addition of the microorganism source) twice a week, followed by addition of soup boiled in bonito and washing with tap water. This one-compartment cultivation was referred to as the "inoculation · boiled broth" broth.

In addition, in the container filled with the rock-bottom cube, a compartment without the microorganism source was used as a control experiment of a 'non-inoculated · boiled broth' sphere.

Also, as a control experiment of the 'chemical fertilizer' sphere, the compartment in which the chemical fertilizer was added instead of the liquid containing the organic matter was added to the container filled with the rocky cube described above, without adding the microorganism source. The results are shown in Fig.

As a result, the cheongcheongchae of the 'inoculation · bonito broth' of the present invention showed good growth equivalent to that of the 'chemical fertilizer'.

In addition, in the "non-inoculated · boiled broth" soup (ie, a cultivar grown on a rocky cube containing soup added with bonito soup without inoculating a microorganism source), the growth of cheongchyeongchae was greatly delayed. This is thought to be due to the fact that the decomposition of the boiled broth was not proceeded only by the ammonia formation because the microorganism source was not inoculated, and the ammonia excess disorder occurred.

Example 13 (Growth experiment using catalyst column effluent)

100 ㎎ of bonito broth broth was mineralized as a microbial carrier on a rocky granular surface, and 100 ml of the effluent was used for growth experiments.

100 ㎎ of bonito broth was added to the catalyst column packed with the rock surface with the microbial groups immobilized thereon, which was obtained in Example 2, and the mixture was allowed to stand overnight at room temperature (about 25 캜) 100 ml was added and the effluent was recovered (treatment for producing nitric acid nitrogen as an inorganic fertilizer component from the organic material) was repeated for one day.

100 ml of the effluent obtained each day was used as a fertilizer, and the operation of providing the seedlings to the seedlings of 10 liters of seedlings was repeated every day (and at this time, the balance from the previous day was discarded). The seedlings were allowed to stand under the conditions of day and night at 25 캜, natural light.

As a control experiment, the operation of providing 100 ml of distilled water containing 100 mg of boiled broth was repeated every day. The results are shown in Fig.

As a result, even when a effluent containing nitrate nitrogen produced from organic matter is used as fertilizer by a catalyst column filled with a solid carrier (rock face granular surface) immobilized with a microorganism group performing a parallel doubly mineralizing reaction, And it was found that it grew. In the control experiment, the growth of the litter did not progress substantially, and the growth disorder considered to be an excess of ammonia disorder appeared.

Example 14 (Use of a solid carrier immobilized with a microorganism group as a microorganism source 1)

It has been investigated whether or not the solid carrier itself in which the microorganism group that has already undergone the simultaneous doubly-charged mineralization immobilization can be used as a microorganism source.

The bottom of the PET bottles for 500 ml was cut to obtain the upper half of the bottle, and the upper bottle and the lower bottle were reversed while the lid was opened to prepare a container in which the lid of the PET bottles was drained.

1 g of a rock face granule surface already obtained by immobilizing the microorganism group (which has reached to catalyze a parallel-doubling mineralization reaction) obtained in Example 1 was added as a microorganism source and inoculated (the volume of the final rock face granular surface was 50 ml), distilled water (100 ml) was added, and the granular phase was washed out from the drainage port.

Thereafter, as an organic substance, 0.1 g of boiled broth was added, and the mixture was allowed to stand overnight at 37 占 폚 and then washed with distilled pure water (100 ml) until the number of days shown in Fig. 22 elapsed. At the time of washing, the effluent after washing was recovered and nitrate ion concentration was measured.

As a result, it was found that nitrate ions were produced at a concentration of about 20 mg / L after 6 days and about 35 mg / L after 8 days when the rock surface was immobilized on the rock surface of the microorganism group obtained in Example 1 .

Therefore, it has been shown that the microorganism carrier immobilized with the microorganism group can be used as a microorganism source to an additional unused solid carrier.

Example 15 (Use of solid carrier immobilizing microorganism group as microbial source 2)

It has been investigated whether a solid carrier immobilized with a microorganism group that has already undergone the simultaneous doubly-charged mineralization reaction can be used as a microorganism source of a parallel doubly-mineralized reaction in water.

100 mL of distilled pure water was placed in an Erlenmeyer flask having a capacity of 500 mL and the soil was added as a microorganism source in Example 7 to prepare a rock surface granule having already been immobilized with the microorganism group immobilized 1 g of cotton was taken out and added as a microorganism source and inoculated.

As an organic matter, 0.1 g of boiled soup broth was added and cultured at a water temperature of 25 캜 until the number of days shown in Fig. 23 elapsed while maintaining the aerobic condition by shaking at 120 rpm to obtain a nitrate ion concentration The measurements were performed sequentially. The results are shown in Fig.

From these results, it has been shown that a solid support in which a group of microorganisms that have already undergone the simultaneous doubly mineralization reaction has been immobilized can be used as a microorganism source to carry out a parallel double ionization reaction in water.

The present invention enables application to inorganic fertilizer production technology using organic waste as a raw material. The waste recycling industry is expected to expand to a market size of 2.5 trillion yen in the future, and the present invention is the first technology to recycle the organic resources as inorganic fertilizer, and the industrial applicability is very large.

In the prior art, most of the energy is required for the aeration, so the power of the air pump or the like is required. In the present invention, by adopting the column system, it is possible to realize an energy- One point is also increasing its appeal.

Further, the present invention makes it possible to cultivate a nutrient solution using an organic fertilizer by a solid distribution system, and can be expected to be applied to agriculture and horticulture. Particularly, in the case of tomato nutrient solution cultivation, it is expected that the solid culture medium by the rocky surface is the main subject, and when it becomes possible to apply the nutrient solution cultivation utilizing the organic fertilizer, it will be spread to countries with high environmental consciousness such as the Netherlands as well as Japan. In particular, the tomato cultivation in the Netherlands is more than 1,000 ha, and the market size is large even if this part is replaced with the nutrient solution for organic fertilizer.

Brief Description of Drawings

1 is an explanatory diagram showing an embodiment of a method for immobilizing a microorganism group with a solid carrier.

Fig. 2 is an explanatory diagram showing an embodiment of a bioreactor optimized for a parallel-doubled mineralization reaction.

3 is a photographic top view showing a state in which a solid carrier is filled in a container having a drain hole;

FIG. 4 is a photographic top view showing a state where various solid carriers are filled in a container having a drain port. FIG.

5 is a graph showing the measurement results of nitrate ion concentration in Example 1. Fig.

6 is a graph showing the measurement results of nitrate ion concentration in Example 2. Fig.

7 is a graph showing the measurement results of nitrate ion concentration in Example 3. Fig.

8 is a graph showing the measurement results of nitrate ion concentration in Example 3. Fig.

9 is a graph showing the results of nitrate ion concentration measurement in the case of using a seed oil as an organic matter in Example 4. Fig.

10 is a graph showing the measurement results of nitrate ion concentration in the case of using CONSTITLEQUA (CSL) as an organic substance in Example 4. FIG.

11 is a graph showing the measurement results of nitrate ion concentration in Example 5. Fig.

12 is a graph showing the measurement results of nitrate ion concentration in Example 6. Fig.

13 is a graph showing the measurement results of the nitrate ion concentration when seawater and tap water are used as a source of microorganisms in Example 7. Fig.

14 is a graph showing the measurement results of the nitrate ion concentration when the soil is used as the microorganism source in Example 7. Fig.

15 is a graph showing the measurement results of nitrate ion concentration in Example 8. Fig.

16 is a graph showing the measurement results of the nitrate ion concentration in Example 9. Fig.

17 is a photographic top view showing various solid carriers in Example 10. Fig.

18 is a graph showing the measurement results of nitrate ion concentration in Example 10. Fig.

19 is a graph showing the measurement results of nitrate ion concentration in Example 11. Fig.

Fig. 20 is a photographic top view showing the growth state of the blueberry seedlings in Example 12. Fig.

Fig. 21 is a photographic top view showing the growth state of the suit litter in Example 13; Fig.

22 is a graph showing the measurement results of nitrate ion concentration in Example 14. Fig.

23 is a graph showing the measurement results of nitrate ion concentration in Example 15. Fig.

Claims (18)

Adding a microorganism group for carrying out a simultaneous doubling mineralization reaction in which a container is filled with a solid carrier having permeability; and an inorganic substance is added to the container so as to produce nitrate nitrogen, followed by addition of an organic substance, And the solid carrier is allowed to flow out of the solid carrier to allow the nitrate nitrogen to be produced in the effluent to be started to be formed, thereby fixing the microorganism group to be subjected to the simultaneous doubly mineralization reaction, A method for producing a solid carrier immobilized on a microorganism group to be reacted. The method according to claim 1,
Wherein the container is a container having a drain port and the effluent is an effluent discharged from the drain port. Adding a microorganism group that performs nitrate nitrogen formation to the nitrate nitrogen by mineralizing the organic matter, and then adding the organic matter to the solid carrier having the air permeability so as to maintain the solid shape; And the solid carrier is allowed to flow out from the solid carrier to thereby allow the microorganism group to be subjected to the parallel enrichment reaction to be immobilized when the solid carrier is washed, until the nitrate nitrogen starts to be produced in the effluent, A method for producing a solid carrier immobilized with a group of microorganisms that undergoes a doubly-mineralized reaction. 4. The method according to any one of claims 1 to 3,
The nitrate nitrogen is not started to be produced in the effluent flowing out when the solid carrier is washed; then, the organic substance is added, and then the mixture is allowed to stand, and water is added to flow out from the solid carrier, Is repeatedly carried out until the nitrate nitrogen starts to be produced in the effluent flowing out during the washing. 5. The method according to any one of claims 1 to 4,
And said step is carried out until nitric acid ions of 50 mg / L or more are generated in said effluent. 6. The method according to any one of claims 1 to 5,
Wherein the solid carrier is at least one selected from the group consisting of rock wool, vermiculite, pearlite, zeolite, sand, gumumato, glass, ceramic, urethane, nylon, melamine resin, wood chips, rice straw, , Polyacrylamide gel, and agar. The solid carrier according to claim 1, wherein the solid carrier is a porous solid carrier. 7. The method according to any one of claims 1 to 6,
By adding water to the solid carrier to wash the solid carrier to remove nitrate nitrogen, and adding the organic substance to the solid carrier by adding water to the solid carrier, Wherein the ammonia-forming reaction and the nitrifying reaction proceed in the solid carrier without accumulation of the intermediate product, and no denitrification reaction occurs. 8. The method according to any one of claims 1 to 7,
Wherein the microorganism group that performs the parallel doubly mineralization reaction is a culture solution obtained by culturing a microorganism group that performs a parallel doubly mineralization reaction, a dried cell of the microorganism obtained by drying the culture solution, a solid support on which the microorganism group is immobilized, Wherein the solid carrier is added with water to form an aqueous solution of at least one member selected from the group consisting of effluent discharged from the solid carrier, soil, tap water, lake water, springs, well water, And a microorganism group that performs a nitrification property. The method for producing a solid carrier according to claim 1, wherein the microorganism is a microorganism. 9. The method according to any one of claims 1 to 8,
Wherein the amount of the organic substance is 0.1 to 10 g with respect to 1 L of the carrier. 10. The method according to any one of claims 1 to 9,
Wherein the organic substance is a nitrogen-containing organic substance having a C / N ratio of not more than 19 in which the content ratio of carbon and nitrogen is not more than 19. The method according to claim 10, wherein the high nitrogen-containing organic matter is selected from the group consisting of boiled broth, fish meal, oil cake, food waste, corn steep liquor, rice bran, soybean meal, plant residue, milk, By weight of the solid carrier. 11. A catalyst for producing nitrate nitrogen as an inorganic fertilizer component from an organic substance, characterized by using the solid carrier obtained by the method according to any one of claims 1 to 11 as a carrier of a catalyst column for carrying out a bilateral double- ≪ / RTI > A fertilizer comprising nitrate nitrogen which is an inorganic fertilizer component produced using the catalyst column obtained by the method of claim 12. A method for cultivating a plant using the nitrate nitrogen-containing fertilizer according to claim 13. A process for producing a plant-grown solid medium, characterized in that the solid carrier obtained by the method according to any one of claims 1 to 11 is used as a plant-grown solid medium. A plant-grown solid medium obtained by the method of claim 15. A method of cultivating a plant in which organic fertilizer is directly added and cultivated by using the plant-cultured solid medium of claim 16. The method according to claim 14 or claim 17, wherein the plant is a vegetable, vegetable, flower, tree, or fruit plant for harvesting vegetables, fruits of leafy vegetables.

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