KR20110027293A - Cosmetic compound with excellent moisturizing and dermis strengthening effect - Google Patents

Abstract

PURPOSE: A cosmetic composition with effects of moisturizing and enhancing dermis is provided to enhance components of dermis and tissue and to prevent moisture loss. CONSTITUTION: A cosmetic composition contains 0.3-10 weight% of Adansonia Digitata extract, 0.03-10 weight% of Cassia Angustifolia extract, and Pisum Sativum extract as active ingredients. The cosmetic composition additionally contains 0.03-10 weight% of Helianthus Annuus extract as an active ingredient. The cosmetic composition is manufactured in the form of solution, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, wax foundation, or spray.

Description

Cosmetic composition with excellent moisturizing and dermis strengthening effect

The present invention relates to a cosmetic composition excellent in moisturizing and dermal reinforcing effect, by using a natural extract to strengthen the components and tissues of the dermis to inhibit moisture loss from the dermis and inhibit the evaporation of moisture in the epidermis, at the same time moisturizing effect It relates to a cosmetic composition that can provide.

The skin of the human body plays a variety of roles such as protecting the body from external physical, chemical or biological stimuli, and suppressing evaporation of moisture from inside the skin to maintain the homeostasis of the human body. The skin also protects the body from harmful ultraviolet rays from the sun and plays a role in regulating body temperature.

The skin is a sensory organ with a variety of functions that can communicate with the external environment and prevent invading pathogens. Furthermore, the condition and appearance of the skin play an important role as an indicator that can easily recognize the state of health. As such, the skin is indispensable for living as a part of the body that is directly affected by physical, chemical or biological injuries from various external factors, as well as having the ability to heal their functional and structural damage. However, the skin is damaged by various contaminants and stress. If the cell proliferation is not properly performed due to such damage, the skin is damaged more and more rapidly, the elasticity of the skin is rapidly reduced, moisture is lost and wrinkles are caused. Will be generated.

On the other hand, as the skin ages, the amount of collagen, mucopolysaccharide, hyaluronic acid, etc. in the matrix, which plays an important role in skin elasticity, decreases, and the amount of insoluble collagen increases. This makes it difficult for the dermis to keep the skin hydrated and the skin rough. In addition, the function of the sebaceous glands and sweat glands is also reduced, and the amount of sebum and sweat is gradually reduced. As a result, the sebaceous membrane is not properly formed, and the protection against skin hydration is lost. As a result, the skin becomes dry and translucency is lost, thereby reducing the elasticity of skin proteins and generating wrinkles. On the other hand, if the skin's moisturizing function does not function, it can cause skin elasticity. In other words, the loss of moisturizing skin is an important factor of skin aging.

Histological or biochemical studies of the role of the skin is important for the stratum corneum of the epidermis. Figure 1 schematically shows the cross-section of the skin, the stratum corneum is keratinocyte (keratinocyte) keratinocytes of the basal layer of the skin is transferred to the skin epidermal layer while inducing division and differentiation, nuclei are removed and keratin specific proteins accumulate Produces major lipid components of the epidermis. These final keratinocytes are called corneocytes, which form a structure that is uniformly arranged in flat scales between lipids. This structure creates a problem in which a series of structures is destroyed in atopy or aged skin, which fails to function as a stratum corneum, resulting in skin dryness and infection. The destruction of the stratum corneum may occur not only due to disease or aging, but also due to pollution or stress, which is commonly exposed to modern people. Moisture is recognized as a basic property of skin care products because it is easily induced even when excessive detergents are used.

The most commonly used moisturizing ingredients are polyhydric alcohols or polysaccharides, and the moisturizing effect of polyhydric alcohols and polysaccharides is that the hydroxyl groups thereof are hydrophilic and applied to the skin to absorb moisture from the outside of the skin It acts as a maintenance mechanism. However, these moisturizing ingredients have the disadvantage of not showing a sticky feeling and a continuous moisturizing effect, and in addition to affecting the formulation or stability when contained in a large amount in the product, expensive polysaccharides can reduce the price competitiveness.

On the other hand, the dermis located under the epidermal layer is composed of fibroblasts and extracellular matrix (ECM), and this structure provides the elasticity and flexibility of the skin to prevent wrinkles of the skin. The major components of the extracellular matrix consist of proteins such as collagen and elastin and glycosaminoglycans (GAG), a kind of polysaccharides. Glycosaminoclycans are known to act as supporters for the proper shape of tissues, as a signaling material that transmits signals between cells, and to specifically bind to receptors on the cell surface. It also functions as a water absorbent. One of the causes of aging skin dryness and loss of elasticity is the reduction of glycosaminoglycans.

However, conventional cosmetics have a limit in obtaining skin moisturizing effect and dermal strengthening effect, and when chemicals are added to improve the skin, there is a problem in that harmful side effects such as toxicity, skin allergy, skin trouble, etc. are generated.

One aspect of the present invention to provide a cosmetic composition comprising a natural extract that can protect the skin by maintaining the moisture in the epidermis by minimizing moisture loss by strengthening the components and structure of the dermis.

According to one aspect of the invention, there is provided a cosmetic composition comprising an baobab ( Adansonia Digitata ) extract, Cassia Angustifolia extract and pea extract ( Pisum Sativum ) as an active ingredient.

The cosmetic composition preferably further comprises a sunflower ( Helianthus Annuus ) extract as an active ingredient.

The baobab ( Adansonia Digitata ) extract is extracted from the leaves of the baobab, the Indian senna and pea extract is preferably extracted from the seed.

The sunflower ( Helianthus Annuus ) extract is preferably extracted from sunflower seeds.

The cosmetic composition is based on the total weight of the cosmetic composition 0.3% to 10% by weight of baobab ( Adansonia Digitata ) extract, 0.03% to 10% by weight of Cassia Angustifolia extract and 0.03% by weight of pea extract ( Pisum Sativum ) It is preferable to include% to 10% by weight as an active ingredient.

The cosmetic composition preferably comprises 0.1 wt% to 10 wt% of sunflower ( Helianthus Annuus ) extract based on the total weight of the cosmetic composition as an active ingredient.

The cosmetic composition is preferably formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray.

The cosmetic composition is formulated as a body emulsion, body moisturizer, hand cream, foot cream, sun block, facial foam cleanser or body wash. It is preferable.

The cosmetic composition of the present invention strengthens the components and tissues of the dermis to inhibit moisture loss from the dermis, inhibits evaporation of moisture in the epidermis, and may contain moisture, and may provide a moisturizing effect while simultaneously strengthening the dermis. .

According to the present invention, the moisturizing effect, and there is provided a cosmetic composition having a dermal strengthening effect, the cosmetic composition of the present invention baobab (Adansonia Digitata) extract, Indian senna tree (Cassia Angustifolia) effective to extract and pea (Pisum Sativum) extract It is included as an ingredient or sunflower ( Helianthus Annuus ) extract further comprises as an active ingredient.

Looking at the components included as an active ingredient in the composition of the present invention for each component.

Baobab tree ( Adansonia Digitata ) is distributed in tropical Africa and is called the tree of life from 5,000 to 6,000 years of age and has an excellent ability to store moisture for survival in dry climates. It has a strong bond with. Baobab tree extract used in the present invention is preferably extracted from the leaves of the baobabs, extracted and purified from the leaves of the baobabs to obtain a mucus component. The leaves of the baobabs are known to contain large amounts of minerals, vitamins, proteins, and flavonoids useful for the skin. The active ingredient, mucus, not only helps to moisturize, but also forms a protective film on the surface of the skin and has the property of improving flexibility and soft skin feel.

On the other hand, the baobab extract is preferably hydrolyzed, and when the beta-glucosidase-type glycolytic enzyme is treated in the baobab extract, a richer amount of mucus is obtained as well as its stability. You can increase it considerably.

Cassia Angustifolia is a legume shrub that grows in parts of India, Arabia and Africa. The indosenna tree extract used in the present invention is preferably extracted from the seed of the indosenna tree, and the seed extract of the indosenna tree is based on a vegetable heteropolysaccharide (heteropolysaccharide), similar to hyaluronic acid. It has a chain structure. This structural property is hydrophilic, helps to form a protective film, and when used as a skin care composition to moisturize and feel a soft feeling on the skin and induces the effect of improving skin luster.

Pisum Sativum extracts, especially extracts from peas, which are the seeds of peas, are the major plant peptide actives that inhibit the proteolytic enzymes elastinase and collagenase found in the dermis. It is known to have a function of, and also to show a radical scavenger. Such effects may not only protect and repair proteins of skin tissues, but may also provide anti-aging effects by giving skin flexibility.

Sunflower ( Hlianthus Annuus ), especially the active ingredient extracted from sunflower seeds is composed of lipids with properties similar to the lipid components in the skin. Representative ingredients include phytoceramide-6 and beta-sitosterol. It has properties similar to the lipids present in the skin barrier, which inhibits evaporation of water from the epidermis and can quickly repair broken skin barriers. The composition of the present invention further comprises a sunflower seed extract as described above is enhanced the moisturizing effect.

The extract of the present invention can be extracted by any suitable method known in the art, for example, after obtaining and washing the raw materials of each component, the volume of water, ethanol, methanol of about 1 to 30 times the weight of the raw materials , Solvents such as propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, propylene glycol, hydrobutylene glycol, hydrous propylene glycol, or hydrous glycerine are added to extract hot water, reflux circulation, dipping or pressure extraction. After extraction using the extraction method can be obtained by centrifugation, vacuum filtration, concentration and lyophilization, but is not limited to the above extraction method.

2 and 4, the cosmetic composition of the present invention promotes the synthesis of dermal structural proteins such as collagen, a major protein between dermal layer cells, and GAG, a major matrix proteoglycan of the dermal layer. Inhibition of the expression of the cell matrix protease (MMP-1), a major proteolytic enzyme that degrades the matrix, has the effect of strengthening the dermis.

The proteolytic enzyme (MMP-1) present in the skin serves to modify the dermal structure by decomposing elastin or collagen, a structural protein of the dermis, and the proteolytic enzyme of the epidermal layer differentiates, keratinizes, and moisturizes the epidermal keratinocytes. It affects your back. The promotion of the differentiation of keratinocytes of the epidermis, together with the promotion of the production of moisturizing factors of the skin, serves to prevent the evaporation of water from the epidermis, thereby maintaining a healthy epidermis. The protease is regulated activity affects tissue regeneration and growth, etc., the protease is responsible for precisely controlling the proteolytic ability by the presence of inhibitors in each cell. Inadequate control of protein resolution causes various problems on the skin, such as skin troubles associated with aging, decreased elasticity, ultraviolet rays and chemicals repeatedly exposed from the environment. .

In addition, the cosmetic composition of the present invention promotes the differentiation of keratinocytes as shown in Figures 5 and 6, Figure 5 shows the results of morphologically measured differentiation induction experiments of epidermal keratinocytes differentiated keratinocytes ( keratinocyte) has a flat shape compared to the undifferentiated case, and thus, undifferentiated keratinocytes have a depth and volume, so that the color around the cell is observed in the picture. In the case of FIG. 5 (f) treated with the composition of the present invention, since the nuclei are lost and have a flat morphology, they are flatly attached to the bottom, so that the surroundings of the cells are rarely observed in bright colors. Figure 6 shows the results of biochemical measurement of the differentiation-induced experiments of these epidermal keratinocytes. The amount of expression was observed using involuclin, which specifically binds to differentiated cells, wherein actin is a cytoskeletal protein possessing a certain amount of epidermal keratinocytes, and the expression of actin to be corrected with the same amount of protein in the experiment. The amount was quantified in the same experiment. Figure 7 shows the device for experimenting with the artificial skin moisturizing effect of the cosmetic composition of the present invention.

To this end, the cosmetic composition of the present invention is based on the total weight of the cosmetic composition of about 0.3% to about 10% by weight of baobab ( Adansonia Digitata ) extract, preferably about 0.03% to about 10% of Cassia Angustifolia extract % By weight and about 0.03% to about 10% by weight of Pisum Sativum extract as an active ingredient. Preferably the cosmetic composition further comprises about 0.1% to about 10% by weight of the sunflower ( Helianthus Annuus ) extract based on the total weight of the cosmetic composition as an active ingredient. It is difficult to expect the efficacy of the present invention when the components are included in amounts less than the lower limit of each described content, and if included in an amount exceeding the upper limit of each described content, problems may occur in the formulation of the cosmetic composition.

The cosmetic composition of the present invention may include the above-mentioned extracts as an active ingredient, and may appropriately mix the remaining ingredients other than the active ingredient according to various formulations.

Cosmetic compositions according to the invention can be prepared in any formulations conventionally prepared in the art, for example solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing It may be formulated as cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, the present invention is preferably prepared in leave-on formulations such as body emulsions, body moisturizers, hand creams, foot creams, sunblocks and the like. It may also be prepared as a rinse-off formulation, such as a Facial Foam Cleanser or a body wash.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Further, if necessary, phenoxyenthanol, hydroxyethylecellulose, carbomer, glycerin, sodium citrate, chlorphensin, potassium sorbate sorbate, methylparaben, octyldodecanol, coco-glycerides, butylparaben, tocopheryl acetate, etc., commonly used in the art. It may include a component of.

More specifically, the cosmetic composition of the present invention is about 1% by weight of baobab ( Adansonia Digitata ) extract and about 0.5% of Cassia Angustifolia extract based on the total weight of the cosmetic composition when formulated as a body emulsion. Weight percent, about 0.2 weight percent of Pisum Sativum extract, and about 0.3 weight percent of Helianthus Annuus extract;

When formulated as a body moisturizer, about 1% by weight of the Adansonia Digitata extract, about 0.5% by weight of Cassia Angustifolia extract, and pea extracts of Pisum Sativum based on the total weight of the cosmetic composition About 0.1% by weight, about 0.3% by weight of Helianthus Annuus extract;

When formulated as a hand cream, about 1% by weight of the extract of Adansonia Digitata , about 0.5% by weight of Cassia Angustifolia extract, about 0.1% by weight of Pisum Sativum extract, based on the total weight of the cosmetic composition, Helianthus Annuus extract comprises about 0.5% by weight;

When formulated as a foot cream, about 1% by weight of the Adansonia Digitata extract, about 0.5% by weight of Cassia Angustifolia extract, and about 0.1% of Pisum Sativum extract based on the total weight of the cosmetic composition Weight percent, about 0.1 weight percent sunflower ( Helianthus Annuus ) extract;

When formulated as a sunblock , about 0.3% by weight of baobab ( Adansonia Digitata ) extract, about 0.03% by weight of Cassia Angustifolia extract, about 0.03% by weight of Pisum Sativum extract, based on the total weight of the cosmetic composition, Helianthus Annuus extract comprises about 0.14% by weight;

When formulated as a Facial Foam Cleanser, about 0.3% by weight of the Adansonia Digitata extract, about 0.03% by weight of Cassia Angustifolia extract, and peas ( Piesum Sativum ) based on the total weight of the cosmetic composition About 0.03 weight% extract, about 0.14 weight% Helianthus Annuus extract;

When formulated as a body wash, about 0.3% by weight of the Adansonia Digitata extract, about 0.03% by weight of Cassia Angustifolia extract, and about the extract of Pisum Sativum based on the total weight of the cosmetic composition It is preferable to include 0.03% by weight and about 0.14% by weight of sunflower ( Helianthus Annuus ) extract.

Hereinafter, the present invention will be described in more detail with reference to specific examples, and the following examples are provided to more specifically describe the present invention, and the present invention is not limited by the following examples.

Example

1. Extraction of Active Ingredients

Baobab ( Adansonia Digitata ) extract was heated 20 kg of purified water to 70 ℃, and then crushed 2.0 kg Baobab leaves were added to the temperature again to 90-95 ℃ extracted for 1 hour. After cooling the extract to room temperature, it was hydrolyzed using beta-glucosidase form of glycolysis. Thereafter, the crude extract obtained therefrom was centrifuged at 5,000 g of centrifugal force to obtain a final extract as a supernatant. The hydrolysis process enables to extract a greater amount of mucus components from the leaves of the baobab and has the effect of increasing the stability of the extract.

Cassia Angustifolia extract is dried after seeding of Indian Senna tree, and then 1 kg of powdered powder is placed in 5 L of water and boiled for 5 hours using an extractor with a cooling condenser (90 ° C), followed by 400 mesh Filtration was carried out with a filter cloth and used after cooling to room temperature.

Pesum ( Pisum Sativum ) extract is dried powder of 1 kg of dried peas into 5 L of water and extracted using a extractor with a cooling condenser for one day at 60 ° C., filtered through a 400 mesh filter cloth and cooled to room temperature. Used.

Sunflower ( Helianthus Annuus ) extract was collected from the dried sunflower seeds and 1 kg of powdered powder was extracted for 24 hours at room temperature using 5 L octyldodecanol. Thereafter, the extract was used after filtering with a 400 mesh filter cloth.

2. Dermal Strengthening Experiments in Cells

(1) Determination of collagen

Baobab tree extract, indone senna tree extract, pea extract and sunflower extract, which are components of the cosmetic composition of the present invention, may be appropriately mixed in different ratios according to the final formulation of the cosmetic composition within the content range of the present invention, and thus each extract The compositions of the following can be prepared separately to facilitate mixing in various proportions in various cosmetic compositions.

Baobab (Adansonia Digitata) including a baobab (Adansonia Digitata) phenoxyethanol (phenoxyenthanol) 2% by weight based on the weight of the extraction composition, carbomer (carbomer) 0.3% by weight and the balance baobab (Adansonia Digitata) extract An extraction composition was prepared.

Cassia Angustifolia extract composition was dissolved by dissolving 2.5% by weight of Cassia Angustifolia extract, 2% by weight of phenoxyethanol, and 0.45% by weight of hydroxyethylecellulose in the remainder of purified water. Prepared.

42% by weight of glycerin, 1% by weight of sodium citrate, 0.3% by weight of chlorphensin, 0.5% by weight of potassium sorbate, based on the weight of the Pisum Sativum extract composition A Pisum Sativum extract composition was prepared containing 0,2% by weight of methylparaben and the remainder of the purified Pisum Sativum extract.

16 wt% coco-glycerides, 12 wt% sunflower seed extract, 0.05 wt% butylparaben, and 0.05 wt% tocopheryl acetate, based on the weight of the sunflower ( Helianthus Annuus ) seed extract composition An extract comprising a sunflower ( Helianthus Annuus ) seed extract composition comprising a) and a balance of octyldodecanol was prepared.

In the present experiment, three extract compositions comprising only 0.3 wt% of the indosenna tree extract composition, 1 wt% of the baobab tree extract composition, and 0.2 wt% of the pea extract composition, each of which consists of the balance of the cell culture medium, were used as an active ingredient. Each of the cosmetic composition of the present invention containing all of the above components as an active ingredient Fibroblasts derived from human dermis were treated for 2 days in cultured medium to grow to about 80% of the culture dish area, and the culture solution was collected.

In order to quantify collagen in the collected culture, each reaction was performed with Circol, a dyeing reagent that specifically binds to collagen. After sufficiently reacting the collagen and dyeing reagents, the collagen conjugate combined with the dyeing reagent is separated by centrifugation, and the dyeing solution bound to the collagen is dissolved. It is dissolved in 0.5M NaOH of alkaline solution and its absorbance is measured at 540 nm. It was.

The results are shown in FIG. 2, and the increased amount of collagen was quantified in the composition of the present invention as can be seen in FIG. 2. Based on these results, it can be seen that the cosmetic composition of the present invention significantly promotes the synthesis of collagen as compared with the case of treatment with each individual component only.

(2) Quantification of MMP-1

Fibroblasts derived from human dermis were placed in a culture dish and cultured to grow to about 80% of the culture dish area. Thereafter, as an active ingredient, three compositions comprising only 0.3% by weight of Indian Senna tree extract, 1% by weight of baobab tree extract, and 0.2% by weight of pea extract, the balance consisting of the remaining cell culture medium, were used as a control. Each cosmetic composition of the present invention containing all The cultures were harvested after 2 days of treatment.

An immunochemical assay was used to measure the concentration change of MMP-1 (Matrix metalloprotease), a fibroblast collagenase, the major proteolytic enzyme that degrades the collagen matrix. First, an antibody (anti-MMP-1, GE Healthcare) that specifically reacts with MMP-1 is reacted with a cell culture medium, and then a secondary antibody (peroxy purified from radish) that reacts with MMP-1 bound to the antibody. Recoloring reagent (TMB substrate, 3,3 ', 5,5'-tetramethylbenzindine / hydrogen peroxide solution, GE Healthcare) which reacts to Days-bound anti-rabbit antibody (GE Healthcare) again and reacts to the enzyme labeled on the secondary antibody The amount of the MMP-1 in the culture solution was measured to the extent of color reaction.

The experimental results are shown in FIG. 3, and as can be seen from FIG. 3, it can be seen that the composition of the present invention significantly inhibits MMP-1 expression as compared to the case of treatment with each individual component only.

(3) Determination of glycosaminolycans (GAG)

Fibroblasts derived from human dermis were placed in a culture dish and cultured to grow to about 80% of the culture dish area. Thereafter, as an active ingredient, three compositions comprising only 0.3% by weight of Indian Senna tree extract, 1% by weight of baobab tree extract, and 0.2% by weight of pea extract, the balance consisting of the remaining cell culture medium, were used as a control. After treating the cosmetic composition of the present invention containing all for two days, the culture solution was collected.

In order to quantify glycosaminoglycans of the collected culture, each reaction was performed with Blycan, a dyeing reagent that specifically binds to glycosaminoglycans. After sufficiently reacting the glycosamino lycan and the dyeing reagent, the glycosamino lycan conjugate combined with the dyeing reagent is separated by centrifugation and dissolving the dissolving reagent (dissociation reagent). It was dissolved in a component of the Blycan assay kit, Biocolor Ltd.) and its absorbance was measured and measured at 656 nm.

The results are shown in FIG. 4, and as can be seen in FIG. 4, an increased amount of lycosamino lycans (GAG) was quantified in the composition of the present invention. Based on these results, it can be seen that the cosmetic composition of the present invention significantly promotes the synthesis of lycosamino lycans compared with the case where the individual cosmetic components are treated only with each individual component.

As can be seen in the above experiments, the cosmetic composition of the present invention increases the production of collagen and GAG in the dermis and suppresses the expression of MMP-1, thereby helping to form a firm dermal structure and enhancing the function of the barrier membrane, thereby showing a dermal strengthening effect. As a result, the moisture inside the skin can be prevented from leaking out, the skin is strengthened, elasticity and flexibility can be given to protect the skin healthily.

3. Experiment of differentiation induction of epidermal keratinocytes

(1) Morphological Differentiation Measurement

Keratin cells derived from humans were cultured to grow about 90% of the culture area in the culture dish. Then, as an active ingredient, three compositions comprising only 0.5% by weight of Indian Senna tree extract (d), 1% by weight of Baobab tree extract (c), and 0.2% by weight (e) of pea extract, and consisting of the balance of the cell culture medium, and Untreated (a) as a negative control, 1.2 mM CaCl ₂ (b) was treated as a positive control to confirm that the cell differentiation is normally induced, the cosmetic composition of the present invention containing all of the above components as an active ingredient (f) ) 2 days after each treatment to determine the degree of differentiation of keratinocytes by observing the cell morphology and the results are shown in FIG. 5 (Eclipse TS100 microscope, Digital Sight camera Nikon).

Inducing differentiation of keratinocytes as can be seen in the cell morphology shown in FIG. 5 shows a differentiation-specific morphology of a flat shape, which cannot be confirmed when treated with each individual component only (c, d, e). While differentiation occurred, apparent differentiation was observed in the case of treatment with the composition of the present invention (f).

(2) biochemical differentiation measurement

To biochemically measure the differentiation of epidermal keratinocytes, the expression level of involucrin, which is a biomarker of keratinocyte differentiation, was confirmed by Western method and the results are shown in FIG. 6.

As can be seen in Figure 6 (a) and Figure 6 (b), when treated with the composition of the present invention as compared to the case of treatment with only individual components, it was possible to confirm the significant expression of involukrin, which is keratin The cells are markedly differentiated. At this time, actin is a cytoskeletal protein having a certain amount of epidermal keratinocytes, and the amount of actin expression was quantified in the same experiment to correct the same amount of protein in the experiment.

As can be seen from the above results, the cosmetic composition of the present invention promotes the differentiation of keratinocytes of the epidermis, which promotes the production of moisturizing factors of the skin and at the same time serves to prevent the evaporation of the skin from the epidermis and thus the healthy epidermis. Can be maintained.

On the other hand, the dual effect of the dermal strengthening effect and keratinocyte differentiation can bring simultaneously the benefits of improved moisturizing effect and elasticity and flexibility than the moisturizing to inhibit epidermal water evaporation.

4. Measurement of epidermal moisture evaporation in artificial skin

As shown in FIG. 7, the artificial skin (VitroSkin N-19, IMS inc) was cut to an appropriate size and fixed to completely cover the container containing a predetermined amount of purified water. On the other side of the artificial skin containing purified water, it contains only 0.5% by weight of Indian Senna extract, 1% by weight of Baobab tree extract, 0.2% by weight of Pea extract, and 0.3% by weight of sunflower extract, respectively. ethanol) was dissolved in a mixture of the remainder of the ratio of 7: 3 and four compositions and untreated spheres were used as a control, and after applying a certain amount of the cosmetic composition of the present invention containing all of the above components as an active ingredient, the temperature was 27 ℃, 50% humidity Water evaporation was induced at. After inducing water evaporation for 3 days, the weight of purified water remaining in the container was measured, and the degree of epidermal water evaporation was compared with the weight of purified water before the experiment, and the results are shown in FIG. 8.

As can be seen in Figure 8, when the composition of the present invention was applied as compared to the case of treatment with only individual components, it was confirmed that effectively inhibit the evaporation of water. The cosmetic composition of the present invention can exhibit excellent moisturizing effect by inhibiting moisture loss in the dermis and epidermis, thereby increasing elasticity and flexibility of the skin as well as moisturizing.

1 schematically shows the cross section of the skin.

Figure 2 shows the results of collagen quantification experiments to confirm the collagen synthesis capacity of the composition of the present invention.

Figure 3 shows the results of the MMP-1 quantitative experiment for confirming the ability to inhibit MMP-1 expression of the composition of the present invention.

Figure 4 shows the results of glycosaminoglycan (GAG) quantitative experiment to confirm the glycosaminoglycan (GAG) synthesis ability of the composition of the present invention.

Figure 5 shows the results of morphologically measured differentiation-induced experiments of epidermal keratinocytes.

Figure 6 shows the results of the biochemical measurement of the differentiation-induced experiment of epidermal keratinocytes.

Figure 7 shows the device for experimenting with the artificial skin moisturizing effect of the cosmetic composition of the present invention.

Figure 8 shows the results of experiments using the artificial skin moisturizing effect of the cosmetic composition of the present invention.

Claims (8)

Cosmetic composition comprising baobab ( Adansonia Digitata ) extract, Cassia Angustifolia extract and Pea ( Pisum Sativum ) extract as an active ingredient. The cosmetic composition of claim 1, wherein the cosmetic composition further comprises a sunflower ( Helianthus Annuus ) extract as an active ingredient. The cosmetic composition of claim 1, wherein the baobab ( Adansonia Digitata ) extract is extracted from the leaves, and the Indian senna and pea extracts are extracted from the seeds. The cosmetic composition according to claim 2, wherein the sunflower ( Helianthus Annuus ) extract is extracted from seeds. According to claim 1, wherein the cosmetic composition is based on the total weight of the cosmetic composition 0.3% by weight to 10% by weight of baobab ( Adansonia Digitata ) extract, 0.03% to 10% by weight of Cassia Angustifolia extract and pea ( Pisum Sativum ) Cosmetic composition comprising 0.03% to 10% by weight of the extract as an active ingredient. The cosmetic composition of claim 2, wherein the cosmetic composition comprises 0.1 wt% to 10 wt% of a sunflower ( Helianthus Annuus ) extract based on the total weight of the cosmetic composition as an active ingredient. 3. The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation Or a cosmetic composition formulated in a spray. 8. The cosmetic composition of claim 7, wherein the cosmetic composition is a body emulsion, a body moisturizer, a hand cream, a foot cream, a sun block, a facial foam cleanser or a body wash. cosmetic composition formulated into a wash).

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