KR101587443B1 - Cosmetic composition for skin anti-aging and anti-wrinkle with the extract of Senna alexandrina and Tinnevellin glucoside - Google Patents
Cosmetic composition for skin anti-aging and anti-wrinkle with the extract of Senna alexandrina and Tinnevellin glucoside Download PDFInfo
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- KR101587443B1 KR101587443B1 KR1020150091441A KR20150091441A KR101587443B1 KR 101587443 B1 KR101587443 B1 KR 101587443B1 KR 1020150091441 A KR1020150091441 A KR 1020150091441A KR 20150091441 A KR20150091441 A KR 20150091441A KR 101587443 B1 KR101587443 B1 KR 101587443B1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The present invention relates to a cosmetic composition for preventing skin aging and improving skin wrinkles containing Senna alexandrina extract or tinnevellin glucoside as an active ingredient, wherein senna extract or tinebrene glucoside has excellent antioxidative effect, MMP- 1 inhibitory effect. Accordingly, a cosmetic composition containing senna alexandrina extract or tinnevellin glucoside as an active ingredient exhibits excellent skin aging prevention and skin wrinkle-reducing effects.
Description
The present invention relates to a senna (Senna alexandrina) extract, tine Dublin glucoside (tinnevellin glucoside) a cosmetic composition for preventing skin aging and wrinkle containing as an active ingredient.
Recently, as interest in appearance has increased, researches for delaying and preventing skin aging have been actively conducted. As the interest in wrinkles increases, the necessity of cosmetics which can be improved according to the acquired efforts is urgently needed to maintain and improve the elastic skin.
Skin is an important institution that protects the human body from the external environment and plays a variety of physiological functions such as barrier function, body temperature control, excretion, etc., which prevent the internal moisture and useful components from leaking out. However, in general, with age, these functions are gradually weakened and the aging of the skin progresses. Particularly, as the lipid composition and content of the lipid layer functioning as a skin barrier are rapidly changed, the function of the lipid layer deteriorates and the skin moisture content decreases, causing the skin to become dry, spots, freckles, pigmentation and various skin lesions.
The connective tissue involved in the wrinkling of the skin consists mainly of fiber components such as collagen fiber, reticular fiber and elastin fiber. Collagen and elastin in the dermis are important fiber structures that maintain the elasticity and elasticity of the skin by working closely with each other. On the other hand, MMP-1 is one kind of MMP (matrix metalloproteinase) and is a protease present in the skin. It is a protein important for intercellular morphogenesis, wound healing, tissue regeneration and reorganization. However, when exposed to ultraviolet light, MMP-1 is produced in the body more than usual, and collagen is broken after binding with collagen, causing skin aging. More specifically, the collagen proteins bind specifically to the hemopexin domain of the MMP-1 and resolve the tertiary structure of the collagen. The collagen is bound to the zinc ions of the MMP-1 catalytic domain After bonding, it is cut.
On the other hand, Senna ( Senna alexandrina Mill.) Is a kind of herbal medicine which grows in a part of India, Arabia and Africa and grows about 1 m. Leaves are 4 ~ 12 cm long, facing long oval, and leaves are flat. The flower is yellowish in March ~ April, and runs on the flower. The fruit is greenish in early summer with conifers, and becomes brown when dried.
Senna leaves are known to have pharmacological effects such as diet and digestion, hemorrhoids, jaundice, anemia and constipation, and the effect as a cosmetic composition is known to be effective for acne and inflammatory skin because of its excellent antibacterial activity. However, most of the existing studies using senna in this way are for herbal medicine treatment, and the effect as a cosmetic composition is insufficient. In addition, the effect of preventing the skin aging and improving wrinkles of the cosmetic composition containing senna extract has not been disclosed in the related art.
It is an object of the present invention to develop and provide a cosmetic composition containing Senna alexandrina extract, tinnevellin glucoside as an active ingredient, exhibiting excellent skin aging-preventing effect and excellent wrinkle-reducing effect.
In order to achieve the above object, the present invention provides a cosmetic composition for preventing skin aging, which comprises Senna alexandrina extract as an active ingredient. Senna extract exhibits excellent antioxidative and MMP-1 inhibitory effects, and is excellent in cell survival rate and stability, and thus is suitable for application to skin cosmetic compositions.
The senna extract may be extracted from any one or more of leaves, stems, and roots of senna, but is most preferably extracted from leaves.
Meanwhile, the Senna extract may be extracted with an extraction solvent selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, and butyl acetate, and one or more mixed solvents as an extraction solvent .
In addition, the Senna extract used in the present invention includes not only the extract obtained by the above-mentioned extraction method, but also the extract obtained through a conventional purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, Fractions are also included in the extract of the present invention.
In addition, the senna extract of the present invention can be produced in a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.
On the other hand, the senna extract is preferably contained in an amount of 0.00001 to 50.0% by weight based on the total weight of the cosmetic composition. If the amount is less than 0.00001% by weight, the effect of preventing skin aging and improving the wrinkles of the skin is insignificant. If the amount is more than 50.0% by weight, it affects the shape and stability of the product.
On the other hand, the present invention provides a cosmetic composition for preventing skin aging comprising tinnevellin glucoside as an active ingredient. Tinblin glucoside exerts excellent antioxidative and MMP-1 inhibitory effects, is excellent in cell survival rate and stability, and is suitable for application to skin cosmetic compositions. Tinneblin glucoside has a structure represented by the following general formula (1) and has not been disclosed in the prior art for a cosmetic composition which contains tinbrene glucoside as an active ingredient and exhibits excellent skin aging prevention and skin wrinkle improving effect.
[Chemical Formula 1]
The above-mentioned tineblinked glucoside may preferably be derived from Senna alexandrina .
On the other hand, the above-mentioned tinebrine glucoside is preferably contained in an amount of 0.00001 to 50.0% by weight based on the total weight of the cosmetic composition. If the amount is less than 0.00001% by weight, the effect of preventing skin aging and improving the wrinkles of the skin is insignificant. If the amount is more than 50.0% by weight, it affects the shape and stability of the product.
On the other hand, in the cosmetic composition of the present invention, the skin aging may preferably be caused by wrinkles.
Meanwhile, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, But are not limited to, water-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.
When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan, and the like.
In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.
When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.
When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.
In the present invention, the term " contains as an active ingredient "means that Senna extract or tinebulin glucoside is added to the composition to such an extent that it can exhibit skin aging-preventing effect and skin wrinkle-reducing effect from the cosmetic composition of the present invention.
According to the present invention, the Senna alexandrina extract of the present invention or tinnevellin glucoside exerts excellent antioxidative and MMP-1 inhibitory effects. Accordingly, senna (Senna alexandrina) cosmetic composition containing an extract or a tine Dublin glucoside (tinnevellin glucoside) as an active ingredient exerts an excellent anti-aging and skin wrinkle improvement effect.
1 is an HPLC chromatogram of Senna extract of the present invention.
FIG. 2 shows the results of confirming cell survival rate and MMP-1 inhibition rate of senna extract.
FIG. 3 shows the results of confirming the cell survival rate and MMP-1 inhibition rate of tinbrene glucoside.
Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.
[ Example One: Senna Preparation of extract]
The dried senna leaves were finely cut, and 1 kg of dry weight was added to the flask and extracted with 10 ℓ of 70% ethanol for 3 days. After extraction, the residue was filtered to remove 157.3 g of senna extract.
Thereafter, the components of the above-prepared Senna extract were analyzed using HPLC. Senna extract was dissolved in methanol and passed through a 0.45 ㎛ PVDF syringe filter (Whatman, Maidstone, England), and then the surface components were analyzed using an HPLC apparatus (Waters, USA). Acetonitrile (A) and water (B) were used for the HPLC grade and the mobile phase was 0 ~ 10 minutes for Senna extract, 10% A; 10 to 30 minutes, 10 to 100% A; 30 to 31 minutes, 100 to 10% A; And linear gradient was performed under the conditions of 31 to 40 minutes and 10%. The flow rate was 1.0 ml / min (acetonitrile, water) and the sample injection amount was 10 μl. At this time, the profile of the index component of the senna extract was represented by a chromatogram of 280 nm as a positive peak, and the result was as shown in Fig. 1 is an HPLC chromatogram of Senna extract of the present invention.
[ Example 2: Senna From the extract Thinabine glucoside ( tinnevellin glucoside ) detach]
In this Example, tinbrene glucoside was separated from Senna extract prepared in Example 1.
130 g of the senna extract prepared in Example 1 was suspended in purified water and then fractionated with n-hexane to obtain 5.04 g of n-hexane fraction. The water layer was further fractionated with methylene chloride To give 10.39 g of methylene chloride fraction. Thereafter, the aqueous layer was further fractionated with ethyl acetate to obtain 4.9 g of ethyl acetate fraction. The ethyl acetate fraction (2 g) was subjected to reverse phase silica gel column chromatography (MeOH: Water gradient) and divided into five fractions (SAE-1 to SAE5). 2.2 mg of tinnevellin glucoside was then isolated from SAE-2 using preparative HPLC and confirmed to remain at 16.3 min.
[ Experimental Example One: Senna The extract and Thinabine glucoside ( tinnevellin glucoside )of Radical scavenging ability (DPPH method) Evaluation of antioxidant use]
In this experiment, the antioxidant activity of the senna extract prepared in Example 1 and the tinbrene glucoside prepared in Example 2 was evaluated.
DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals, which are typical in antioxidant assays, have a maximum absorbance at 540 nm in an organic solvent. The antioxidants dissolve the DPPH radicals and lose their intrinsic color and turn yellow .
For the measurement of DPPH radical scavenging, the senna extract prepared in Example 1 and the tinbrene glucoside prepared in Example 2 were adjusted to final concentrations of 125 ppm, 250 ppm, 500 ppm, and 1000 ppm, respectively, . Each diluted sample and ethanol were placed in a 96-well plate, and DPPH radicals were added thereto, followed by reaction for 20 minutes. Thereafter, the absorbance at 540 nm was measured, and the radical scavenging effect was calculated using the following equation (1). Thereafter, a standard curve for each concentration was drawn to calculate the IC 50 (
As a result, Senna extract showed 50% inhibition of active oxygen species even at a low concentration of 191.5 ppm, indicating excellent antioxidative ability. Furthermore, it was confirmed that thinabine glucoside inhibited 50% of active oxygen species at a concentration of 1000 ppm or more.
[ Experimental Example 2: Senna The extract and Thinabine glucoside ( tinnevellin glucoside ) ≪ / RTI >
In this experiment, the effect of the senna extract prepared in Example 1 and the tinbrene glucoside prepared in Example 2 on the cell survival rate was examined.
Human dermal fibroblast neonatal (HDFn) was inoculated in a 96-well plate at a concentration of 1 × 10 4 cells / well and stabilized in a 37 ° C. incubator for 24 hours to stabilize. Senna extracts were treated with 6.25, 12.5, 25, 50, 100 ㎍ / ㎖, and thinabine glucoside was treated with 25, 50, 100, 200 ㎍ / ㎖. After 24 hours of treatment, 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) reagent was treated. Two hours after the treatment, the sample was removed and 150 mu l of DMSO was added to each well to dissolve the formed formazan, and the absorbance was measured at 540 nm. At this time, UVA 6 J / cm 2 And treated with 1 mM L-ascorbic acid as a positive control.
As a result of the measurement, senna extract and tinbrene glucoside showed excellent cell viability (FIGS. 2 and 3). FIG. 2 shows cell survival rate and MMP-1 inhibition rate of senna extract, and FIG. 3 shows cell survival rate and MMP-1 inhibition rate of tinbrene glucoside.
[ Experimental Example 3: Senna The extract and Thinabine glucoside ( tinnevellin glucoside )of MMP -1 inhibition rate]
In this Example, the inhibition of MMP-1 by the senna extract prepared in Example 1 and the tinnitus glucoside prepared in Example 2 was evaluated.
Human dermal fibroblast neonatal (HDFn) was inoculated into a 24-well plate at a concentration of 5 × 10 4 cells / well, and then stabilized in a 37 ° C. incubator for 24 hours. Then, after rinsing twice with PBS, UVA was irradiated with 6 J / cm 2 in the presence of PBS. Then, the cells were treated with senna extract (6.25, 12.5, 25, 50, 100 ㎍ / ㎖), thinabine glucoside (25, 50, 100, 200 ㎍ / ㎖) and positive control with the medium supplemented with 0.2% FBS Treated with 1 mM L-ascorbic acid. After 48 hours, the supernatant was measured at 450 nm using a human total MMP-1 ELISA kit (human total MMP-1 Cat. No. DY901, R & D systems). After the measurement, the inhibition rate of MMP-1 was measured using the following equation.
As a result of the measurement, it was confirmed that both senna extract and tinebrene glucoside inhibit MMP-1 in a concentration-dependent manner. Senna extract showed a superior MMP-1 inhibitory rate than that of the positive control, L-ascorbic acid, from a concentration of 12.5 占 퐂 / ml or more (Fig. 2), and tinbrene glucoside ) Than MMP-1 (FIG. 3). FIG. 2 shows cell survival rate and MMP-1 inhibition rate of senna extract, and FIG. 3 shows cell survival rate and MMP-1 inhibition rate of tinbrene glucoside.
[ Formulation Example 1 and 2: Senna The extract and Thinabine glucoside ( tinnevellin glucoside ) To prevent skin aging Cosmetics Composition Preparation]
In this formulation example In order to prepare cosmetic compositions each containing senna extract or tinebrene glucoside, the b) image recorded in Table 2 below was heated and stored at 70 캜. This was followed by preliminary emulsification, followed by uniform emulsification with a homomixer, followed by gradual cooling to produce a cream-type skin aging-resistant cosmetic composition, which was referred to as Formulation Example 1 and Formulation Example 2, respectively.
(Unit: parts by weight)
Claims (9)
The tinbrene glucoside may be,
A cosmetic composition for preventing skin aging characterized by Senna alexandrina .
The tinbrene glucoside may be,
A cosmetic composition for preventing skin aging characterized by containing 0.00001 to 50.0% by weight based on the total weight of the cosmetic composition.
Skin aging,
A cosmetic composition for preventing skin aging characterized by wrinkles.
In the cosmetic composition,
Characterized in that it is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays A cosmetic composition for preventing skin aging.
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| KR1020150091441A KR101587443B1 (en) | 2015-06-26 | 2015-06-26 | Cosmetic composition for skin anti-aging and anti-wrinkle with the extract of Senna alexandrina and Tinnevellin glucoside |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210006151A (en) | 2019-07-08 | 2021-01-18 | 주식회사 팜스킨 | A composition for blue light interception comprising Senna alexandrina extract |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002255733A (en) * | 2001-02-28 | 2002-09-11 | Alps Yakuhin Kogyo Kk | Useful material using sennoside extraction residue as active ingredient |
| KR20110027293A (en) * | 2009-09-10 | 2011-03-16 | 유한킴벌리 주식회사 | Cosmetic compound with excellent moisturizing and dermis strengthening effect |
| KR20120042281A (en) | 2010-10-25 | 2012-05-03 | 애경산업(주) | Hair cosmetic composition |
| KR101477891B1 (en) | 2013-02-20 | 2014-12-30 | 한울환경연구원(주) | A Composition of Platanus orientalis L extract for anti whitening, wrinkle, aging |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002255733A (en) * | 2001-02-28 | 2002-09-11 | Alps Yakuhin Kogyo Kk | Useful material using sennoside extraction residue as active ingredient |
| KR20110027293A (en) * | 2009-09-10 | 2011-03-16 | 유한킴벌리 주식회사 | Cosmetic compound with excellent moisturizing and dermis strengthening effect |
| KR20120042281A (en) | 2010-10-25 | 2012-05-03 | 애경산업(주) | Hair cosmetic composition |
| KR101477891B1 (en) | 2013-02-20 | 2014-12-30 | 한울환경연구원(주) | A Composition of Platanus orientalis L extract for anti whitening, wrinkle, aging |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210006151A (en) | 2019-07-08 | 2021-01-18 | 주식회사 팜스킨 | A composition for blue light interception comprising Senna alexandrina extract |
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