KR20110007299A - A growing method of bean sprouts containing polyphenols - Google Patents
A growing method of bean sprouts containing polyphenols Download PDFInfo
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- KR20110007299A KR20110007299A KR1020090064752A KR20090064752A KR20110007299A KR 20110007299 A KR20110007299 A KR 20110007299A KR 1020090064752 A KR1020090064752 A KR 1020090064752A KR 20090064752 A KR20090064752 A KR 20090064752A KR 20110007299 A KR20110007299 A KR 20110007299A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
Description
본 발명은 폴리페놀 성분이 함유한 콩나물의 재배방법에 관한 것으로, 상세하게는 참나무로부터 추출된 폴리페놀을 물과 함께 희석한 수용액을 콩이 발아하여 콩나물로 성장하는 과정 중에 충분히 살포하여 살균처리 및 충분한 영양원 공급될 수 있도록 성장을 촉진시킬 수 있는 폴리페놀이 함유한 콩나물의 재배방법에 관한 것이다.The present invention relates to a cultivation method of soybean sprouts containing a polyphenol component, and in particular, an aqueous solution of polyphenol extracted from oak with water is sprayed sufficiently during the process of soybean germination and growth into bean sprouts. The present invention relates to a cultivation method of bean sprouts containing polyphenols that can promote growth to provide a sufficient nutrient source.
일반적으로 콩나물은 콩을 물에 불려 발아시킨 다음 성장용기에 넣고 주기적으로 물을 공급하여 재배한다.In general, bean sprouts are grown by soaking soybeans in water, germinating them in growth containers, and periodically supplying water.
그런데, 이러한 일반적인 콩나물의 재배방법은 콩나물의 성장을 저해하는 균이 왕성하게 번식할 수 있는 환경을 제공함으로써, 성장과정에서 균에 의해 콩나물 머리 부분에 검은 반점이 생기거나, 뿌리 또는 줄기를 부패하게 하거나, 줄기가 멍들어서 성장을 억제함으로써 콩나물의 품질이 저하되고 수확량이 감소하는 문제점이 있다.However, such a general method of cultivating bean sprouts provides an environment in which bacteria that inhibit the growth of bean sprouts can proliferate, causing black spots on the head of the bean sprouts, or decaying roots or stems. Or, there is a problem in that the quality of the bean sprouts are lowered and the yield is reduced by bruising the stems to suppress the growth.
이러한 문제점을 개선하기 위해 종래에는 농약을 주로 이용하였으나, 재배된 콩나 물에 잔류된 농약이 인체에 유해하다는 문제점 때문에 최근에는 인체에 무해한 천연 살균제를 이용하여 생육하는 방법이 다양하게 제안되고 있다.In order to improve such a problem, conventionally, pesticides were mainly used, but recently, various methods of growing using natural fungicides harmless to the human body have been proposed because of the problem that the pesticides remaining in the cultivated beans or water are harmful to the human body.
또한, 종래에 콩나물은, 콩, 녹두, 팥 등의 콩류나 해바라기, 브로콜리의 씨앗을 하이포아염소산이나 고온의 물로 살균·팽윤시키고, 이것에 지하수를 하루에 수회씩 살수하여, 1주일정도 암소에서 발아·생육시킨 후에 소비자에게 제공하여 왔다. 이와 같은 콩나물은 원료로서의 콩의 상태에서는 비타민C가 거의 함유되어 있지 않으나, 발아하여 콩나물의 상태가 되면, 비타민C를 함유하게 되며, 그 밖에, 탄수화물, 단백질을 함유하여, 영양야채로서 널리 식용으로 제공되고 있다.In addition, conventionally, bean sprouts sterilize and swell beans, such as beans, green beans, and red beans, seeds of sunflower and broccoli with hypochlorous acid or high temperature water, and spray groundwater several times a day. After germination and growth, it has been provided to consumers. Such bean sprouts contain little vitamin C in the state of soybean as a raw material, but when sprouted and become a state of bean sprouts, they contain vitamin C. In addition, they contain carbohydrates and proteins, and are widely used as food for nutrition. Is being provided.
그러나, 대부분의 콩나물 제조공장에서 실시되고 있는 하이포아염소산에 의한 소독과 살균은 염소를 포함하기 때문에, 소비자뿐 아니라 생산자의 건강에 미치는 영향이나 공장내의 기기의 부식, 더욱이, 염소를 포함하는 처리수를 그대로 배출하고 있는 것 등, 머지않은 장래에 사회문제가 될 것이 예상된다. 또, 재배기간이 길기 때문에 부패하는 것이 많고, 또, 일일의 생산량이 늘어나지 않음에도 불구하고, 재배기간의 단축에 관한 연구에 진전이 없는 것이 실정이다. 그래서, 소비자나 제조업자의 건강에 안전한 살균방법과 재배기간을 단축시킬 수 있는 기술이 요구되고 있다.However, since the disinfection and sterilization by hypochlorous acid, which is carried out in most bean sprouts manufacturing plants, includes chlorine, the effect on the health of not only consumers but also the producers, the corrosion of the equipment in the plant, and the treated water containing chlorine. It is expected to become a social problem in the near future, such as the fact that it is produced as it is. In addition, because of the long growing period, there is a lot of corruption, and despite the fact that the daily production does not increase, there is no progress in the study on the shortening of the growing period. Therefore, there is a need for a method of sterilization that is safe for the health of consumers and manufacturers and a technology that can shorten the growing period.
또한, 콩류에 함유되는 건강증진성분의 상당한 부분은, 발아나 성장과정에서 소비되고, 게다가, 암소에서 재배하기 때문에, 새로운 물질의 합성이 적어서, 일반적인 녹색의 야채와 비교하여 영양가가 낮은 식품으로 여겨지고 있다. 그래서, 기상의 변화에 영향받지 않고, 일정하게 재배되며, 일상적인 식생활에 기여하고 있음에도 불구하고, 극히 낮은 값으로 판매되고 있다.In addition, a significant portion of the health-enhancing ingredients contained in legumes are consumed during germination and growth and, in addition, because they are grown in cows, the synthesis of new substances is less, making them less nutritious than conventional green vegetables. have. Therefore, despite being influenced by the change of the weather, being cultivated regularly and contributing to the daily diet, it is sold at an extremely low value.
이와 같은 사정을 감안하여 본 발명자들은, 콩나물에 소량밖에 함유되어 있지 않으나, 항산화 및 위암 등에 효과가 있다고 알려져 있는 폴리페놀과 비타민C 등의 영양성분이 다량 함유된 콩나물 재배방법에 대한 연구가 진행되고 있다.In view of such circumstances, the present inventors are researching a method for growing soybean sprouts containing a large amount of nutrients such as polyphenols and vitamin C, which are contained only in a small amount in bean sprouts, but are known to be effective in antioxidant and gastric cancer. have.
본 발명은 상기와 같은 요구사항을 해결하기 위하여 창안된 것으로서, 인체에 유익한 천연재료를 이용하여 콩나물의 살균 및 성장을 촉진시킬 수 있는 콩나물 재배방법을 제공하는데 그 목적이 있다.The present invention has been made to solve the above requirements, and an object of the present invention is to provide a bean sprout cultivation method that can promote the sterilization and growth of bean sprouts using natural materials beneficial to the human body.
또한, 동시에 콩나물의 재배 과정중 천연재료를 이용하여 필수 영양성분을 함유하도록 하는 또다른 목적이 있다.In addition, at the same time there is another purpose to contain the essential nutritional ingredients using natural ingredients during the cultivation process of bean sprouts.
이에 본 발명은 콩나물을 섭취하면서 폴리페놀 성분을 콩나물과 동시에 흡수할 수 있으며, 기타 다른 영양성분까지 섭취할 수 있도록 하는 것으로 콩을 발아하는 과정에서부터 콩이 성장하는 과정 중에 참나무에서 추출한 폴리페놀성분을 물과 함께 희석한 수용액을 살수하여 콩나물에 다량 폴리페놀 성분이 함유할 수 있도록 하는 폴리페놀 성분이 함유한 콩나물의 재배방법을 제공하는 것이다.Therefore, the present invention can absorb the polyphenols and bean sprouts at the same time while ingesting soybean sprouts, so that other nutrients can be ingested, so that the polyphenols extracted from the oak during the process of germinating beans and growing beans It is to provide a method of cultivating bean sprouts containing a polyphenol component so that a large amount of polyphenol components in the sprouts by sprinkling the aqueous solution diluted with water.
이상에서 살펴 본 바와 같은 본 발명에 따른 폴리페놀 성분이 함유한 콩나물의 재배방법에 의하면, 폴리페놀 수용액의 살균력에 의해 성장저해를 억제하며, 미량원소의 함유량이 높아지고 외관은 종래의 콩나물과 동일하면서도, 건강증진에 유용한 물질이 보다 많이 함유되어 있는 콩나물을 재배할 수 있는 것이고, 폴리페놀 수용액을 살수함에 따라 특성상 미립물질을 흡착하여 제거하는 성질에 의하여 콩에 잔류하는 농약이나 중금속 성분을 흡착하여 제거할 뿐만 아니라 발아에 유해한 미 생물은 흡착하여 제거하고 발아에 유익한 미생물은 활성화시킴에 따라 콩나물의 성장을 촉진시킬 수 있을 뿐 아니라 각종 비타민과 무기질 성분들이 다량 흡수되어 영양분이 고르게 함유된 고품질의 콩나물을 제공할 수 있으며, 참나무의 그윽한 향미(香味)가 콩나물 고유의 향미(香味)와 잘 어우러져 보다 맛있는 콩나물을 제조할 수 있는 것으로서 콩나물의 대외 경쟁력을 최대한 높여줄 수 있는 등의 이점이 있는 것이다.According to the cultivation method of the bean sprouts contained in the polyphenol component according to the present invention as described above, by inhibiting the growth inhibition by the sterilizing power of the polyphenol aqueous solution, the content of trace elements is high and the appearance is the same as the conventional sprouts It is possible to grow soybean sprouts containing more substances useful for health promotion and to adsorb and remove pesticides or heavy metal components remaining in soybeans by adsorbing and removing particulate matters by sprinkling aqueous polyphenol solution. In addition, by adsorbing and removing microorganisms harmful to germination and activating microorganisms that are beneficial to germination, they not only promote the growth of bean sprouts, but also absorb high amounts of various vitamins and minerals to provide high-quality bean sprouts containing nutrients evenly. It can be served, and the rich flavor of oak is soybean Blending well with the inherent flavor of the herbs (香 보다) to be able to produce more delicious bean sprouts, such as to increase the external competitiveness of the bean sprouts to the maximum.
상기한 목적을 달성하기 위한 본 발명에서 사용되는 참나무에서 추출한 폴리페놀 추출과정을 살펴보면 다음과 같다.Looking at the polyphenol extraction process extracted from the oak used in the present invention for achieving the above object is as follows.
밀폐된 용기 내에 참나무 목재 칩과 수산기(-OH)를 함유한 추출용매를 포화상태 하에서 전처리시간 30초 이상 12시간미만, 처리온도 50℃이상 400℃ 미만, 압력 1kgf/cm2이상 100kgf/cm2미만의 조건으로 전처리하고, 상기 전처리된 목재를 120℃에서 15분 이상 60분 미만으로 고온 추출 처리한 다음, 여과 및 분리정제를 수행하여 폴리페놀 성분을 함유한 추출액을 제조하는 일반적인 과정을 거친 참나무 폴리페놀 추출액을 제조하게 된다.Extraction solvent containing oak wood chips and hydroxyl group (-OH) in airtight container in a sealed container under saturation condition, pretreatment time 30 seconds or more and less than 12 hours, treatment temperature 50 ℃ or more and less than 400 ℃, pressure 1kgf / cm2 or more and less than 100kgf / cm2 Conditioned, oak polyphenols undergoing the general procedure of extracting the pretreated wood at 120 ° C. for at least 15 minutes at a high temperature of less than 60 minutes, followed by filtration and separation purification to produce extracts containing polyphenol components. An extract is prepared.
또한, 상기 참나무에서 추출한 폴리페놀 추출액 대신에 쑥의 건조분말을 물에 현탁시키고, 상기 현탁액을 효소처리하기 위해 펙틴분해효소인 4ppm의 펙티나아제를 첨가하여, 50~70℃에서 30~90분 동안 교반하면서 반응시킨 후 90~100℃에서 30~90분동안 열수추출하고, 이를 여과하여 액상성분인 폴리페놀 추출물을 수득하는 공정을 포함하는 쑥으로부터 폴리페놀 추출물을 제조하게 된다.In addition, instead of the polyphenol extract extracted from the oak, the dry powder of mugwort was suspended in water, and 4 ppm of pectinase, a pectinase, was added to enzymatically treat the suspension, and then 30 to 90 minutes at 50 to 70 ° C. After the reaction with stirring for a while, hot water extract for 30 to 90 minutes at 90 ~ 100 ℃, and to produce a polyphenol extract from the mugwort comprising the process of obtaining a liquid polyphenol extract by filtration.
또한, 상기 쑥 대신 생(生)솔잎을 스팀기에 넣어 50~80℃에서 30~90분 정도 찐 다음, 상기 찐 솔잎을 효소처리하기 위해 펙틴분해효소인 4ppm의 펙티나아제와 1.5%(w/v)의 홍고추추출물을 첨가하여, 50~70℃에서 30~90분동안 교반하면서 반응시킨 후 90~100℃에서 30~90분동안 열수추출하고, 이를 여과하여 액상성분인 폴리페놀 추출물을 수득하는 공정을 포함하는 생솔잎으로부터 폴리페놀 추출물을 제조하게 된다.In addition, put the raw pine needles in the steamer instead of the mugwort steamed for 30 to 90 minutes at 50 ~ 80 ℃, and then to the enzyme treatment of the steamed pine needles 4ppm pectinase and 1.5% (w / v) red pepper extract was added and reacted with stirring at 50-70 ° C. for 30-90 minutes, followed by hot water extraction at 90-100 ° C. for 30-90 minutes, followed by filtration to obtain a polyphenol extract as a liquid component. The polyphenol extract is prepared from raw pine needles comprising the process.
상기 펙티나아제와 함께 홍고추추출물을 처리한 경우에는, 폴리페놀의 추출수율이 더욱 증가되는 상승효과를 나타냄을 알 수 있으며, 항산화효과를 갖는 비타민 C가 가장 풍부한 홍고추추출물을 처리할 경우에는, 추출물에 존재하는 당의 산화가 방지되므로, 추출물의 갈변화가 억제되고, 환원당의 함량이 증가되는 결과를 초래할 뿐만 아니라, 폴리페놀의 추출수율이 증대되는 효과를 나타낼 수 있다. 이때, 홍고추추출물의 처리양은 특별히 이에 제한되지 않으나, 생솔잎에 대하여 1.0 내지 1.8%(w/v)의 양으로 처리함이 바람직함을 알 수 있었다. 1.0%(w/v)보다 적은 양으로 처리할 경우에는 홍고추추출물을 처리하지 않은 상태와 비교하여 환원당의 함량이 증가되지 않았고, 1.0 내지 1.8%(w/v)의 양으로 처리할 경우에는 처리양에 비례하여 환원당의 함량이 증가되었으며, 1.8%(w/v)보다 많은 양으로 처리할 경우에는 더 이상 환원당의 함량이 증가되지 않음을 알 수 있었다.When the red pepper extract is treated with the pectinase, it can be seen that the extraction yield of the polyphenol shows a synergistic effect. When the red pepper extract rich in vitamin C having the antioxidant effect is treated, the extract is extracted. Since the oxidation of the sugar present in the is prevented, the browning of the extract is suppressed, resulting in an increase in the content of the reducing sugar, as well as can increase the extraction yield of polyphenols. At this time, the treatment amount of red pepper extract is not particularly limited to this, it can be seen that it is preferable to treat in an amount of 1.0 to 1.8% (w / v) with respect to fresh pine needles. When the amount was less than 1.0% (w / v), the content of reducing sugar was not increased in comparison with the untreated red pepper extract. The content of reducing sugar was increased in proportion to the amount, and it was found that the content of reducing sugar was no longer increased when treated with an amount greater than 1.8% (w / v).
즉, 본 발명은 도 1의 공정 블럭도에서 보는 바와 같이 우선, 사용할 원료 콩을 하루 전에 미리 세척하고, 세척한 콩을 선별한 후 6~8℃ 저온상태에서 보관하여 표면의 수분을 제거하고, 콩 자체의 품도를 10℃ 이하로 유지케 한다. 이때 세척한 콩은 내부에 수분을 함유하게 되어 약간 말랑말랑한 상태가 된다.That is, the present invention, as shown in the process block diagram of Figure 1, first to wash the raw beans to be used in advance one day, and then to sort the washed beans stored at 6 ~ 8 ℃ low temperature to remove the surface moisture, The quality of the beans themselves is kept below 10 ℃. At this time, the washed soybeans will contain moisture in the inside will be slightly soft.
이렇게 세척, 선별 후 저온에서 보관한 콩을 1일차에 물과 참나무 폴리페놀추출액이 500:1의 비율로 희석한 폴리페놀수용액에 4~6시간 불리는 공정을 하는 제1단계와,After washing and sorting, the first step of performing a process called 4-6 hours in a polyphenol aqueous solution in which water and oak polyphenol extracts were diluted at a ratio of 500: 1 on day 1 of the beans stored at low temperature;
상기 제1단계 다음으로 콩의 물기를 제거하기 위해 소분한 후 2일차에 20~25℃의 재배실에서 16~18℃ 정도의 온도를 갖는 지하수를 4시간 간격으로 살수하는 제2단계와,A second step of spraying ground water having a temperature of about 16 to 18 ° C. in a planting room at 20 to 25 ° C. on a second day after subdividing so as to remove water from the beans after the first step;
상기 제2단계 다음으로 3~5일차에 물과 참나무 폴리페놀추출액이 500:1의 비율로 희석한 폴리페놀수용액을 1일 1~3회 살포하는 제3단계를 거쳐 콩나물을 재배하게 된다.Next to the second step, the sprouts are grown through the third step of spraying the polyphenol aqueous solution diluted with water and oak polyphenol extract at a ratio of 500: 1 on the 3rd to 5th day.
상기 제3단계 6일부터는 제2단계를 반복적으로 행하는 과정을 거쳐 날짜에 따른 어린콩나물에서 성장된 콩나물에 이르기까지 소비자의 성향에 따른 콩나물을 얻을 수 있게 된다.From the third step 6 days through the process of repeatedly performing the second step it is possible to obtain the bean sprouts according to the consumer's propensity from the young bean sprouts to the grown bean sprouts according to the date.
상기와 같이 원료 콩을 불리는 과정에서는 온도가 콩나물의 성장에 밀접한 관계가 있다. 콩 자체에 묻어 있는 세균 및 곰팡이가 콩나물 성장과정에 최대한 억제되도록 하기 위해서는 불리는 과정에서의 콩의 온도와 폴리페놀수용액의 불림시간을 준 수하여 콩나물 내에 폴리페놀 수율의 효과는 최대화될 수 있도록 한다.In the process called raw soybeans as described above, the temperature is closely related to the growth of bean sprouts. In order to ensure that the bacteria and fungi in the bean itself are suppressed as much as possible in the bean sprout growth process, the soybean temperature and soaking time of the polyphenol solution in the soaking process should be followed to maximize the effect of the yield of polyphenols in the bean sprouts.
성장과정 3~5일째에 폴리페놀수용액을 분무토록 함으로써 재배 후 성장을 저해하는 발병을 최소화시킬 수 있으며, 발병을 예방하게 됨과 동시에 유효한 폴리페놀 성분이 콩나물 내에 잔류할 수 있도록 극대화 하는 시기가 된다.By spraying the aqueous polyphenol solution on the 3rd to 5th days of the growth process, it is possible to minimize the onset of inhibiting the growth after cultivation, and to prevent the onset and maximize the effective polyphenol component to remain in the bean sprouts.
특히 본 발명은 콩에 지하수를 살수하기 전까지 폴리페놀수용액을을 1~3회 분무토록 함으로써 발아 콩나물에 대한 항균 효과 및 폴리페놀 수율함량에 최대화 시킬 수 있다.In particular, the present invention can be maximized in the antimicrobial effect and polyphenol yield content for germinating bean sprouts by spraying the polyphenol aqueous solution 1 to 3 times before the ground water is sprayed on soybeans.
또한, 상기 제1단계 다음으로 보리새싹분말이 포함된 발아배지(보리새싹분말+부직포) 상(上)에 불린 콩을 놓아 1~3.5cm의 발아콩을 재배하는 단계를 더 포함할 수 있으며, 발아시키는 과정에서 수분 살수를 위해 지하수를 사용하여 4시간 간격으로 살수하는 것이 바람직하다.In addition, after the first step, by placing the soybeans on the germination medium (barley sprout powder + non-woven fabric) containing the barley sprout powder may further comprise the step of cultivating germinated beans of 1 ~ 3.5cm, In the germination process, it is preferable to spray water at intervals of 4 hours using ground water for water spraying.
상기 보리새싹 속에 함유된 비타민 A, C, D, E, K와 Se(셀레늄)등 천연 항산화제가 다이옥신과 같은 해로운 환경 호르몬을 이기는 힘을 길러주는 SOD(활성산소 제재물질)을 가지고 있어 콩을 발아시키는 과정에서 상기 보리새싹에 함유된 영양성분을 흡착하여 콩나물 내에 이와 같은 성분이 충분히 포함하도록 하였다.Natural antioxidants such as vitamins A, C, D, E, K and Se (selenium) contained in the barley sprouts have SOD (Active Oxygen Sanctions) that develops the power to overcome harmful environmental hormones such as dioxin. In the process of adsorbing the nutrients contained in the barley sprout so as to sufficiently include such components in the bean sprouts.
발아하는 단계 후 과정은 상기 서술한 제2단계 이후 단계를 포함하는 것으로 순차적인 날짜를 제외한 발아 단계 다음을 계산하여 단계별 날일은 동일하게 적용하면 된다.The process after the germinating step includes the above-described second step and then the step after the germination step except for the sequential date is calculated by applying the same day by day.
이로 인해 본 발명에 따라 재배한 폴리페놀이 함유한 콩나물은 일반 콩나물이 지니고 있는 아스파라긴산, 카로틴, 티아민, 비타민 A를 함유할 뿐만 아니라 참나무 등의 폴리페놀 성분도 함유하여, 식자재로서 콩나물 섭취시 적당량의 폴리페놀 성분을 함께 섭취할 수 있도록 해준다. 여러가지 연구결과에 의하면, 폴리페놀 성분은 콜레스테롤 상승 억제효과, 혈압 상승 억제효과, 항산화효과를 가지므로, 본 발명에 따라 재배된 폴리페놀이 함유한 콩나물에서도 이와 같은 효과를 기대할 수 있을 것이다.For this reason, the bean sprouts containing the polyphenols grown according to the present invention not only contain aspartic acid, carotene, thiamine, vitamin A, which are contained in general bean sprouts, but also contain polyphenols such as oak. Allows you to eat with phenols. According to various studies, since the polyphenol component has cholesterol suppression effect, blood pressure increase inhibitory effect, and antioxidant effect, the same effect can be expected in bean sprouts containing polyphenols grown according to the present invention.
도 1은 본 발명에서 제공하는 폴리페놀이 함유한 콩나물 재배방법의 개략적인 공정 블럭도.1 is a schematic process block diagram of a method for growing bean sprouts containing polyphenols provided by the present invention.
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