KR20100044578A - Cosmetic composition for skin anti-aging comprising oligopeptide mixture stabilized in nanoliposome contained in liquid crystal base - Google Patents

Abstract

PURPOSE: A cosmetic composition for skin anti-aging containing an oligo-peptide mixture stabilized on a nano liposome and a liquid crystal base is provided to stably inject the oligopeptide mixture for improving skin-moisture effect and anti-wrinkle effect. CONSTITUTION: A cosmetic composition for skin anti-aging is a mixture containing an oligo-peptide mixture stabilized on a nano liposome and a liquid crystal base produced using lecithin, ceramide, cholesterol or their derivatives. The oligo-peptide mixture is a mixture of hexapeptide, acetyl tetra peptide and pentapeptide. The content rate of the oligo-peptide mixture is 0.1~60.0wt% by the entire weight of the cosmetic composition. The oligo-peptide mixture is contained in 0.01~1wt% by the entire content of the nano liposome. The content rate of the liquid crystal base is 20~99.9wt%.

Description

Cosmetic composition for preventing skin aging containing oligopeptide mixture stabilized in nanoliposomes and natural liquid crystal emulsifying base {{Cosmetic Composition for Skin Anti-Aging Comprising Oligopeptide Mixture Stabilized in Nanoliposome Contained in Liquid Crystal Base}

The present invention is formulated with a mixture of three kinds of oligopeptides having excellent anti-aging effects on the natural liposomes in nanoliposomes to aid in penetration of the active ingredients into the natural liquid crystal emulsifying base, thereby providing excellent anti-aging effects and moisturizing by strengthening the skin barrier. The present invention relates to a cosmetic composition having enhanced effects and wrinkle improvement.

The skin is an organ that exists at the outermost part of the body. It is an important organ that has various functions and has a great influence on appearance and image.It is composed of various cells and unique structures, covering the outer surface of the body to prevent evaporation of moisture. It is the body that plays the role of the body's primary defense against the external environment, such as controlling the temperature, protecting the human body from external stimuli such as ultraviolet rays, and preventing invasion of harmful microorganisms.

The skin is composed of three layers of epidermis, dermis and subcutaneous fat. The outermost epidermis plays the most important role in terms of skin aging, and thus has been the subject of intensive research on skin beauty.

The components that make up the stratum corneum, the outermost layer of the epidermis, are keratinocytes and skin lipids. The skin lipids act as a barrier to the skin, which protects the skin from external harmful substances by controlling cell division and differentiation. It is an important function that prevents the leakage of substances in the body and prevents skin moisture evaporation.

Among the skin lipids, the lipids responsible for the barrier function of the skin are mainly composed of ceramides, cholesterol and free fatty acids, and they account for 40-65%, 10% and 25% of the stratum corneum lipids, respectively.

As such, the components constituting the skin barrier are composed of lipids, the skin surface is covered with a sebaceous membrane, has a lipophilic property, and constitutes a solid skin lipid barrier, so that it is known that the water-soluble effective ingredients are very difficult to penetrate the skin.

In the art, a method of preparing a cosmetic composition by encapsulating an active ingredient in liposomes, capsules, nano-vesicles, etc., in order to increase skin penetration of the water-soluble active ingredients is used. However, these techniques merely make the particles finer to facilitate skin penetration and retain water-soluble properties, so that the problem of completely penetrating the lipophilic skin barrier is not solved.

On the other hand, the recent research to improve the skin wrinkles by applying the peptide component to the cosmetics are actively progress. European Patent No. 1 180524A1 discloses an amino acid sequence excellent in wrinkle improvement of a peptide. In particular, 6 to 19 peptides among amino acid sequences have been found to have an excellent wrinkle improvement effect. Peptides currently used as cosmetic raw materials are acetyl hexapeptide-3 (acetyl glutamyl-glutamyl-methionyl-glutamyl-arginyl-arginylamine (acetyl-Glu-Glu-Met-Glu-Arg -Arg)). Representative.

The present inventors have diligently tried to overcome the problems of the prior art as a result, the cosmetic composition containing a natural liquid crystal emulsified base consisting of a mixture of oligopeptides stabilized in the nano liposomes and a composition similar to skin lipids, the skin anti-aging effect The present invention has been completed by finding excellence.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition for skin protection containing a mixture of oligopeptides stabilized in the nano liposomes and a natural liquid crystal emulsified base.

Another technical problem of the present invention is to provide a cosmetic method, characterized in that the cosmetic composition is applied to human skin.

Other technical objects and advantages of the present invention will become apparent from the following examples and claims.

In order to achieve the above technical problem, the present invention is a natural liquid crystal prepared by (a) a mixture of oligopeptides stabilized in nanoliposomes and (b) lecithin, ceramide, cholesterol or derivatives thereof and phytosterol or derivatives thereof Provided is a cosmetic composition for protecting a skin containing an emulsified base.

The present invention comprises (a) a mixture of oligopeptides stabilized in nanoliposomes and (b) a lecithin or derivative thereof, ceramide, cholesterol or derivatives thereof, and a natural liquid crystalline emulsified base prepared mainly from phytosterol or derivatives thereof. It relates to a cosmetic composition for preventing skin aging. The cosmetic composition of the present invention can maximize the efficacy by stably penetrating the oligopeptide mixture at a high concentration without stimulation, as well as excellent skin moisturizing effect and wrinkle improvement effect.

The present invention comprises (a) a mixture of oligopeptides stabilized in nanoliposomes and (b) a lecithin or derivative thereof, ceramide, cholesterol or derivatives thereof, and a natural liquid crystalline emulsified base prepared mainly from phytosterol or derivatives thereof. It relates to a cosmetic composition for preventing skin aging.

In the cosmetic composition of the present invention, the active ingredient exhibiting skin anti-aging effect is an oligopeptide mixture, which is a mixture of hexapeptide, pentapeptide and acetyltetrapeptide.

In the present invention, the oligopeptide mixture is included in the nanoliposomes and stabilized, and then, when contained in a natural liquid crystal emulsified base prepared with lecithin, ceramide, cholesterol or derivatives thereof, phytosterol or derivatives thereof as a main component, skin protection of the present invention is achieved. The effect can be maximized.

As an active ingredient of the present invention, the hexapeptide is a synthetic oligopeptide characterized by SEQ ID NO: 1 (Ser-Ile-Lys-Val-Ala-Val-OH), and a laminin that enhances the bond between the epidermis and the dermis forming the skin. By promoting the synthesis of -5 and collagen, it is excellent for skin wrinkle improvement and elasticity. In the embodiment of the present invention was used hexapeptide-10 provided by Lipotec (Lipotec).

Next, pentapeptide is a synthetic oligopeptide characterized by SEQ ID NO: 2 (Tyr-Ala-Gly-Phe-Leu-OH), and is a signal shearing substance, Enkephaline, which controls muscle movement by signal transduction. By suppressing the activity of the wrinkles formed by the facial expression is excellent. In the embodiment of the present invention was used pentapeptide-3 provided by Lipotech.

Next, acetyltetrapeptide is a synthetic oligopeptide characterized by SEQ ID NO: 3 (Acetyl-Ala-His-Ser-His-OH), which prevents the elasticity from falling down due to the accumulation of fat around the eye and the degradation of proteins. Very good at removing In the embodiment of the present invention was used acetyl tetrapeptide-5 provided by Lipotech, Spain.

The oligopeptides can be synthesized and commercially sold. In the present invention, it was purchased from Lipotech, Spain.

In the oligopeptide mixture of the present invention, the hexapeptide, pentapeptide and acetyltetrapeptide are mixed in a ratio of 0.0001-1.0: 0.0001-1.0: 0.0001-1.0, preferably in a ratio of 0.005-0.5: 0.005-0.5: 0.005-0.5 Most preferably in a 1: 1: 1 ratio.

As used herein, the term "nano liposome" refers to a spontaneous spontaneous bond and aligned colloidal particles of particles composed of amphiphilic molecules with water-soluble heads and insoluble tails, having a double-membrane structure similar to a cell membrane, and the composition containing the damaged skin. It can be expected to restore the barrier, strengthen the skin barrier, and alleviate skin irritation. According to a preferred embodiment of the present invention, the average particle diameter of the nano liposome is 300 nm, when the average particle diameter of the nano liposome exceeds 1000 nm, improvement of skin penetration and formulation stability among the technical effects to be achieved in the present invention The improvement is very weak.

The content of the oligopeptide mixture stabilized by inclusion in the (a) nanoliposome contained in the cosmetic composition of the present invention is 0.1-60.0 wt%, preferably 0.5-20.0 wt%, based on the total weight of the cosmetic composition. In addition, the oligopeptide mixture is included in 0.001 to 2.0% by weight, preferably 0.01 to 1.0% by weight relative to the total weight of the nano liposomes.

'Natural liquid crystal emulsified base' of the present invention is an oil-in-water (O / W) type mixture having a lamella structure, comprising (1) lecithin or derivatives thereof, (2) ceramide, (3) cholesterol or derivatives thereof, and (4) It contains phytosterol or a derivative thereof as a main component, and is contained in an amount of 20.0-99.9% by weight based on the total weight of the cosmetic composition.

Suitable contents of the natural liquid crystal emulsifying base of the present invention are (1) 0.1-25.0% by weight of lecithin and its derivatives, preferably 0.3-15.0% by weight, (2) 0.1-10.0% by weight of ceramide, based on the total weight of the cosmetic composition. Preferably 0.3-5.0% by weight, (3) 0.1-10.0% by weight of cholesterol or derivatives thereof, preferably 0.3-5.0% by weight, (4) 0.1-10.0% by weight of phytosterol or derivatives thereof, preferably 0.3 It is obtained by adding 5.0% by weight. In addition, higher fatty alcohol or higher fatty acid may be added to strengthen the liquid crystal structure.

Hereinafter, the components of the liquid crystal emulsified base used in the cosmetic of the present invention will be described in more detail. The lecithin used in the present invention includes both lecithin obtained from soybeans, egg yolks, plant seeds and the like and hydrogenated lecithin saturated by saturation thereof. Next, ceramides include sphingosine derived from nature such as synthetic, fermented, yeast and copper, and plants, and derivatives thereof and cerebromide. Cholesterol or its derivatives are made from esters of synthetic and natural cholesterol and cholesteryl alkylates such as cholesteryl stearate and cholesteryl isostearate made by esterification thereto and cholesteryl alkylates such as cholesterol. Reel glutamate, and the like. In addition, phytosterol or its derivatives include phytosterols derived from plants and phytosteryl alkylates such as phytosteryl stearate and phytosteryl isostearate and phytosteryl glutamate obtained by esterifying amino acids to phytosterols. Etc. Higher fatty alcohols are naturally occurring alcohols having 10-30 carbon atoms, and higher fatty acids are naturally occurring fatty acids having 8-30 carbon atoms.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. .

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular, in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention comprises (a) a mixture of oligopeptides stabilized by inclusion in nanoliposomes and (b) a natural liquid crystalline emulsified base prepared mainly from lecithin, ceramide, cholesterol or derivatives thereof, phytosterol or derivatives thereof. As a composition, it is very excellent in moisturizing effect and facial wrinkle improvement effect by strengthening skin barrier, and their skin absorption is very excellent.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1 Preparation of Nanoliposomes Containing Oligopeptide Mixture of the Present Invention

According to the composition of Table 1, to prepare a nano liposome to which the oligopeptide mixture is added as an active ingredient of the present invention. Oligopeptide mixtures used in the preparation of nano liposomes include hexapeptide-10 (trade name: SELILESIME, Lipotech Co.), pentapeptide-3 (trade name: LEUPHASYL, Lipotech Co.) and acetyltetrapeptide-5 (trade name: EYESERYL, Lipotech Co. ) Was used in a 1: 1: 1 mixture.

Preparation of Nanoliposomes (Content: wt%) Prize ingredient Content (% by weight) A Glyceryl Stearate - Cetearyl Alcohol - Polysorbate 60 - Sorbitan sesquioleate - Fiji-100 Stearate - Squalane - Caprylic / Capric Triglycerides - Beta-sitosterol 2.0 lecithin 3.0 Glycolipid 1.0 Hydrogenated phosphatidylcholine 2.0 B glycerin 5.0 Xanthan Gum 0.10 Incense, preservative Quantity Purified water Remaining amount C Oligopeptide Mixtures (1: 1: 1 mixture of hexapeptide-10, pentapeptide-3 and acetyltetrapeptide-5) 2.0

Warm phase A and phase B up to 80 ℃ to make a single solution, mix by adding phase C at 60 ℃, pass through high pressure emulsifier three times at 1000 bar, and then cool and nanoliposomes with an average particle of 300 nm. Was prepared.

Comparative Preparation Examples 1, 2 and 3: Preparation of Nanoliposomes Containing Caffeine

In order to measure the skin penetration of the nano-liposomes of the present invention, the composition of the nano-liposomes of the present invention in the composition of nanoliposomes (Comparative Preparation Example 1) and Table 1 containing caffeine instead of the oligopeptide mixture of C in Table 1 The liposome composition was changed to nanoliposomes containing the caffeine instead of the oligopeptide mixture (Comparative Example 2) and caffeine-containing compositions (Comparative Example 3).

Preparation of nanoliposomes containing caffeine (content: wt%) Prize ingredient Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 A Glyceryl Stearate - 2.0 - Cetearyl Alcohol - 2.0 - Polysorbate 60 - 1.2 - Sorbitan sesquioleate - 0.8 - Fiji-100 Stearate - 1.0 - Squalane - 10.0 - Caprylic / Capric Triglycerides - 10.0 - Beta-sitosterol 2.0 - - lecithin 3.0 - - Glycolipid 1.0 - - Hydrogenated phosphatidylcholine 2.0 2.0 - B glycerin 5.0 5.0 - Xanthan Gum 0.10 0.10 - Incense, preservative Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount C Caffeine 2.0 2.0 2.0

The A and B phases were heated to 80 ° C. to make a single solution, followed by mixing by adding C phase at 60 ° C., and then passing the high pressure emulsifier three times at 1000 bar to cool the nano liposomes. In Comparative Preparation Example 3, only the C phase was dissolved at room temperature to prepare a final product.

Comparative Preparation Examples 4, 5 and 6: Liposomes for comparing the nano liposome effect of the present invention

Comparative Preparation Example 4 having a nanoliposomal composition of the present invention without containing an oligopeptide mixture as an active ingredient for comparing the effects of the nanoliposome of the present invention, Comparative Preparation Example having a conventional liposome composition without containing an oligopeptide mixture Comparative Preparation Example 6 was prepared having a conventional liposome composition while including 5 and an oligopeptide mixture.

As a production method, Comparative Production Example 4 was carried out in the same manner as in Production Example 1. In Comparative Production Examples 5 and 6, the A and B phases were heated and mixed at 75 ° C., respectively, and emulsified.

Prize ingredient Comparative Production Example 4 Comparative Production Example 5 Comparative Production Example 6 A Glyceryl Stearate - 2.0 2.0 Cetearyl Alcohol - 2.0 2.0 Polysorbate 60 - 1.2 1.2 Sorbitan sesquioleate - 0.8 0.8 Fiji-100 Stearate - 1.0 1.0 Squalane - 10.0 10.0 Caprylic / Capric Triglycerides - 10.0 10.0 Beta-sitosterol 2.0 - - lecithin 3.0 - - Glycolipid 1.0 - - Hydrogenated phosphatidylcholine 2.0 2.0 2.0 B glycerin 5.0 5.0 5.0 Xanthan Gum 0.10 0.10 0.10 Incense, preservative Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount C Oligopeptide Mixtures - - 2.0

Preparation Example 2 Manufacturing Method of Natural Liquid Crystal Emulsifying Base

A natural liquid crystalline emulsified base composition consisting of the composition described in Table 4 below, similar to the lipid of the present invention, was prepared.

Prize ingredient Content (% by weight) A Hydrogenated Lecithin 8.0 cholesterol 2.0 Ceramide (03) 1.0 Cetostearyl alcohol 2.0 PEG-5 Soyasterol 2.0 Caprylic / Capric Triglycerides 15.0 ethanol 5.0 B glycerin 10.0 Purified water Remaining amount

The lipid phase (A phase) and the water phase (B phase) were each heated to 80 ° C to form a single solution, and then the aqueous phase was slowly added to the lipid phase and mixed, and then passed through a high pressure emulsifier three times at 1000 bar. Prepared.

Experimental Example 1: Measurement of penetration effect of active ingredient (caffeine permeation amount)

In order to test the penetration effect of the active ingredient of the cosmetic containing a nano liposome and a natural liquid crystal emulsified base of the present invention was carried out as follows.

The cosmetics containing the liposomes used in the experiment and the natural liquid crystal emulsifying base of Preparation Example 2 were prepared by gradually adding the B and C phases in phase A and uniformly mixing according to the composition of Table 5 below. Cosmetics were used.

Preparation of cosmetics (content: weight%) Prize ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 A Natural Liquid Emulsifying Base (Manufacturing Example 2) 80 80 80 B Incense, preservative Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount C Comparative Production Example 1 10 - - Comparative Production Example 2 - 10 - Comparative Production Example 3 - - 10

The skin penetration effect experiment is a vertical diffusion cell (Cell: Frenz Diffusion cell, Labfine, Korea) that measures the amount of material permeation from the epidermis to the liquid medium after fixing the skin of an animal or human to a holder. The skin penetration amount of caffeine contained in Comparative Examples 1, 2 and 3 was measured using. For experiments, 6-week-old male hairless mouse ventral skin was used, the skin area was 0.64 cm ² , the medium used was PBS (pH 7.4), and 0.50 g of Comparative Examples 1, 2, and 3 were applied at 37 ° C., respectively. After reacting for 2 hours, 150 μg of medium permeated through the skin was taken, and the content of caffeine permeated was measured using HPLC. At this time, in order to compare the effective ingredient penetration effect of Comparative Examples 1, 2 and 3 clearly, Comparative Preparation Examples 1, 2 and 3 were also tested together to determine the caffeine content. Based on this evaluation, the content of caffeine (permeated caffeine content (%)) penetrating the skin was calculated according to Equation 1 below, and the results are shown in Table 6 below.

Permeated Caffeine Content (%) product Permeated Caffeine Content (%) Comparative Example 1 Containing Nanoliposomes and Natural Liquid Emulsifying Base of the Present Invention 85% Comparative Example 2 Containing Conventional Liposomes and Natural Liquid Emulsifying Base 65% Comparative Example 3 Containing Natural Liquid Crystal Emulsion Base Only 38% Comparative Preparation Example 1 consisting of the nano liposome of the present invention 52% Comparative Preparation Example 2 consisting of conventional liposomes 41% Comparative Preparation Example 3 Containing No Liposomes and Natural Liquid Emulsifying Base 28%

As can be seen from the results of Table 6, Comparative Example 1, which is a cosmetic containing a nano liposome and a natural liquid crystal emulsified base of the present invention, as well as Comparative Preparation Example 1 or Comparative Preparation Example 2 consisting of the present invention or conventional liposomes, Compared with Comparative Example 2 containing both the conventional liposomes and the natural liquid crystal emulsified base of the present invention and Comparative Example 3 containing only the natural liquid crystal emulsified base, the 'permeated caffeine content' effect was excellent.

Preparation Example 3 Preparation of Cosmetic Composition Containing Oligopeptide Mixture

The cosmetics containing the nano liposome of Preparation Example 1 and the natural liquid crystal emulsifying base of Preparation Example 2 containing the oligopeptide mixture of the present invention were prepared as follows. At room temperature, phases B and C were slowly added and uniformly mixed to complete the final cosmetic product (Example 1). At this time, for comparison with the composition of the present invention Comparative Example 4 containing a nano liposome and a natural liquid crystal emulsification base of the present invention that does not contain an oligopeptide mixture, while containing a conventional liposome and a natural liquid crystal emulsion base Comparative Example 5 containing and Comparative Example 6 containing a conventional liposome and a natural liquid crystal emulsification base without including the oligopeptide mixture was prepared, the preparation method is emulsified after mixing the A, B phases at 75 ℃ warm The mixture was cooled to 40 ° C, and the C phase was added and mixed to obtain a final product.

Prize ingredient Example 1 Comparative Example 4 Comparative Example 5 Comparative Example 6 A Natural Liquid Emulsifying Base (Manufacturing Example 2) 80 80 80 80 B Incense, preservative Quantity Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount Remaining amount Preparation Example 1 10 - - - C Comparative Production Example 4 - 10 - - Comparative Production Example 5 - - 10 - Comparative Production Example 6 - - - 10

Experimental Example 2: Moisturizing effect of the cosmetic of the present invention

In order to evaluate the moisturizing power of the cosmetics prepared in Preparation Example 3 (Example 1 and Comparative Examples 4, 5, and 6), the moisture retention ability of the skin to which each cosmetic was applied was measured as follows.

Each of the cosmetics of Example 1 and Comparative Examples 4, 5, and 6 was divided into half of 36 test subjects' faces in half at a constant temperature and humidity room of 22 ° C and a relative humidity of 45%, and the product of Example 1 was compared to the other. The water content of 4 weeks after was measured, apply | coating the product of Examples 4, 5, and 6 (twice / day), respectively.

As a measuring device, a moisture content measuring instrument (Corneometer CM825, Courage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 8 below.

Water content Example 1 Comparative Example 4 Comparative Example 5 Comparative Example 6 0 Week 71.08 ± 9.34 69.71 ± 11.36 70.25 ± 6.31 70.87 ± 8.04 4 Week 76.78 ± 7.09 73.25 ± 5.58 74.38 ± 5.10 74.01 ± 6.34 4 Week-0Week 5.70 ± 7.07 3.54 ± 5.36 4.13 ± 6.23 3.14 ±± .86

As shown in the above results, the oligopeptide mixture of the present invention was contained in a nanoliposome and stabilized, and the cosmetic composition of Example 1 contained in the liquid crystal emulsified base was stabilized by inclusion of the oligopeptide mixture of the present invention in a conventional liposome. Then, Comparative Example 5, which contains it in the liquid crystal emulsified base, Comparative Example 4, which contains a nano liposome containing no peptide mixture in the liquid crystal emulsified base, it is included in the liquid crystal emulsified base by inclusion It was excellent in the moisture retention power compared with the comparative example 6 contained in the.

Experimental Example 3: Evaluation of the wrinkle improvement effect of the cosmetic of the present invention

Wrinkle improvement effect of each of the cosmetics (Example 1 and Comparative Examples 4, 5, 6) prepared in Preparation Example 3 was evaluated through a practical test.

For 36 women, the product of Example 1 was used on one side of the face and the products of Examples 4, 5, and 6 were used on the other side, respectively. The degree of improvement was evaluated. Wrinkle improvement effect results based on this evaluation are as shown in Table 9 below.

Wrinkle improvement effect product Great slightly none Effectiveness% Example 1 17 4 3 87.5 Comparative Example 4 5 8 11 54.2 Comparative Example 5 15 3 6 75% Comparative Example 6 3 7 14 41.6

As shown in the above results, the oligopeptide mixture of the present invention was contained in a nanoliposome and stabilized, and the cosmetic composition of Example 1 contained in the liquid crystal emulsified base was stabilized by inclusion of the oligopeptide mixture of the present invention in a conventional liposome. Then, Comparative Example 5, which contains it in the liquid crystal emulsified base, Comparative Example 4, which contains a nano liposome containing no peptide mixture in the liquid crystal emulsified base, it is included in the liquid crystal emulsified base by inclusion The wrinkle improvement effect was also excellent compared with the comparative example 6 contained in the.

To summarize the results of the above experimental example, the skin penetration of the active ingredient is better contained in the nano liposomes than the normal emulsified base, and the active ingredient (oligo peptide mixture when making a product using a natural liquid crystal emulsified base rather than the nano liposome alone ) Can be applied to the skin well, so that the effect of moisturizing, improving wrinkles, etc. can be better shown, and skin irritation was not observed in most of the subjects who applied the cosmetic to the skin.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

<110> COREANA COSMETICS CO., LTD. <120> Cosmetic Composition for Skin Anti-Aging Comprising Oligopeptide          Mixture Stabilized in Nanoliposome Contained in Liquid Crystal          Base <160> 3 <170> KopatentIn 1.71 <210> 1 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> hexapeptide-10 <220> <221> MOD_RES <222> (6) Val-OH {Hydroxylation of Valine end} <400> 1 Ser Ile Lys Val Ala Val   1 5 <210> 2 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> pentapeptide-3 <220> <221> MOD_RES <222> (5) <223> Leu-OH {Hydroxylation of Leucine end} <400> 2 Tyr Ala Gly Phe Leu   1 5 <210> 3 <211> 4 <212> PRT <213> Artificial Sequence <220> 223 acetyltetrapeptide-5 <220> <221> MOD_RES <222> (1) <223> Acytyl-Ala {Acetylation of Alanine end} <220> <221> MOD_RES <222> (4) <223> His-OH {Hydroxylation of Histidine end} <400> 3 Ala His Ser His   One  

Claims (7)

A cosmetic composition for preventing skin aging comprising (a) a mixture of oligopeptides stabilized by inclusion in nanoliposomes and (b) a natural liquid crystal emulsified base prepared mainly from lecithin, ceramide, cholesterol or derivatives thereof and phytosterol or derivatives thereof. According to claim 1, wherein the oligopeptide mixture is a skin composition for preventing skin aging, characterized in that the mixture of hexapeptide, penta peptide and acetyl tetrapeptide. According to claim 2, wherein the hexapeptide is a sequence described in SEQ ID NO: 1, the penta peptide is a sequence described in SEQ ID NO: 2, the acetyl tetrapeptide is a sequence described in SEQ ID NO: 3 Composition. The cosmetic composition for preventing skin aging according to claim 1, wherein the content of the oligopeptide mixture stabilized by inclusion in the (a) nanoliposome is 0.1-60.0 wt% based on the total weight of the cosmetic composition.      The cosmetic composition for preventing skin aging according to claim 4, wherein the oligopeptide mixture is contained in an amount of 0.01 -1.0 wt% based on the total weight of the nano liposomes. The cosmetic composition for preventing skin aging according to claim 1, wherein the content of the natural liquid crystal emulsifying base of (b) is 20.0-99.9% by weight based on the total weight of the cosmetic composition. A cosmetic method according to any one of claims 1 to 6, wherein the cosmetic composition of claim 1 is applied to human skin to achieve a skin aging improvement effect.