KR102628359B1 - A cosmetic composition comprising a small liposome liquid crystal emulsion, and a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing comprising the same - Google Patents

Abstract

The present invention provides a cosmetic composition capable of continuous release of effective substances with high skin absorption rate and excellent moisturizing effect by applying a liquid crystal structure to small liposomes, and a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing containing the same.

Description

Small liposome liquid crystal emulsion cosmetic composition, and multifunctional cosmetic for whitening, wrinkle improvement and moisturizing comprising the same {A cosmetic composition comprising a small liposome liquid crystal emulsion, and a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing comprising the same}

The present invention relates to a small liposome liquid crystal emulsified cosmetic composition and a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing containing the same.

The skin is composed of three layers: the epidermis, dermis, and subcutaneous fat. The epidermis, which exists in the outermost layer, plays the most important role in skin aging and is therefore the subject of intensive research. The components that make up the stratum corneum, the outermost layer of the epidermis, are keratinocytes and skin lipids. Skin lipids are responsible for the skin's barrier function. This barrier function regulates cell division and differentiation to protect the skin from external harmful substances. , It can be said to be an important function that prevents the outflow of substances from the body and prevents evaporation of skin moisture.

Among skin lipids, those responsible for the skin's barrier function are mainly composed of cholesterol, ceramide, and free fatty acids. It is known that water-soluble active ingredients are very difficult to penetrate into the skin because the ingredients that make up the skin barrier are composed of lipids, and the skin surface is covered with a sebum film, which has lipophilic properties and forms a solid skin lipid barrier.

Accordingly, in order to increase the penetration of water-soluble active ingredients into the skin, methods of manufacturing cosmetic compositions by encapsulating active ingredients with liposomes, capsules, nanovesicles, etc. have been studied. However, these technologies simply aim to facilitate skin penetration by making the particles fine, but they retain water-soluble properties, so the problem of completely penetrating the lipophilic skin barrier has not been resolved.

Meanwhile, most existing cosmetics attempted to maintain skin elasticity and moisture by applying moisturizing factors or physiologically active substances to the skin. Because the particles of cosmetic compositions, which serve as delivery vehicles for effective substances, have a structure that is vulnerable to external environmental changes, there are limitations in maintaining useful moisture and active substances in the skin for a long time.

Since the liposome structure has a very good affinity with skin tissue and can protect moisture and active ingredients and release them in a sustained manner, liposomes are the most suitable structure to effectively penetrate the skin with moisture and effective bioactive ingredients in cosmetic compositions. This is a known fact, and cosmetics made by multiple emulsification of liposomes are already on the market.

Meanwhile, unlike liposomes as described above, liquid crystal emulsions are also known to have excellent affinity for biological cells, and are described as useful in cosmetic compositions due to maintaining structural stability and excellent moisturizing effect. Cosmetics based on technology are also commercially available.

However, since cosmetics made by emulsifying multiple liposomes are applied to the skin when used, the structure of the liposomes is destroyed before penetrating into the skin, causing the problem that the effect of the liposomes cannot be properly demonstrated.

Accordingly, it has a high skin absorption rate, suppresses the release of moisture and effective substances as much as possible, achieves excellent moisturizing effect and continuous release of effective substances, and at the same time promotes skin cell regeneration and growth, which can have wrinkle improvement and whitening effects. There was a need to develop an improved multi-functional cosmetic composition.

Republic of Korea Patent Registration No. 10-2373899

The present invention provides a cosmetic composition capable of continuous release of effective substances with high skin absorption rate and excellent moisturizing effect by applying a liquid crystal structure to small liposomes, and a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing containing the same.

According to one aspect of the present invention, a cosmetic composition containing an epidermal growth factor (EGF) component and an egg protein component, wherein the epidermal growth factor includes RH-oligopeptide-1, The egg protein component includes hydrolyzed egg protein, albumin extract, egg yolk extract, egg shell membrane extract, and lecithin, and provides a cosmetic composition for whitening, wrinkle improvement, and moisturizing that is prepared by emulsifying small liposome liquid crystals.

The epidermal growth factor component and egg protein component may be stabilized by being encapsulated in small liposomes and released continuously.

The skin absorption rate and absorption rate can be increased by the small liposome liquid crystal emulsification.

RH-Oligopeptide-1, a component of the epidermal growth factor, can stimulate skin cells and promote cell regeneration and growth.

The RH-oligopeptide-1 may be included in an amount of 0.0000001 to 0.0005% by weight based on the total weight of the cosmetic composition, the hydrolyzed egg protein may be included in an amount of 0.00001 to 0.05% by weight based on the total weight of the cosmetic composition, and the albumin extract may be included in an amount of 0.00001 to 0.005% by weight based on the total weight of the cosmetic composition. 0.05% by weight, egg yolk extract may be included at 0.00001 to 0.05% by weight, egg shell membrane extract may be included at 0.00001 to 0.05% by weight, and lecithin may be included at 0.00001 to 0.05% by weight.

The cosmetic composition may further include sorbitan stearate, cetaaryl alcohol, burylene glycol, niacinamide, glycol stearate SE, triethyl citrate, and betaine.

According to another aspect of the present invention, a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing in a serum or cream formulation containing the cosmetic composition is provided.

The cosmetic composition according to the present invention has a high skin absorption rate by applying a liquid crystal structure to small liposomes that are resistant to external environmental changes and can retain moisture and effective substances for a long time, and have excellent performance by suppressing the release of moisture and effective substances as much as possible. In addition to the moisturizing effect, the continuous release of effective substances can provide a long-lasting makeup effect.

In addition, the cosmetic composition according to the present invention has wrinkle improvement and whitening effects by promoting skin cell regeneration and growth due to the inclusion of epidermal growth factor components and egg protein components.

Hereinafter, a cosmetic composition according to specific embodiments of the invention will be described in more detail.

In the existing technology, the problem of cosmetic compositions having difficulty completely penetrating the lipophilic skin barrier has not been resolved, and when using cosmetics using multiple liposome emulsification, the structure of the liposomes is destroyed before penetrating into the skin, thereby demonstrating the effect of the liposomes properly. There was a problem that couldn't be resolved.

Accordingly, the present inventors applied liquid crystal emulsification technology and small liposome technology to increase the skin absorption rate by more than 100 times and stabilize effective substances such as epidermal growth factor ingredients, egg protein ingredients, and ceramide when applying a liquid crystal structure to small liposomes. The invention was completed after confirming through experiments that efficacy could be maximized by capturing and slowly releasing the product.

According to one aspect of the present invention, a cosmetic composition containing an epidermal growth factor (EGF) component and an egg protein component, wherein the epidermal growth factor includes RH-oligopeptide-1, The egg protein component includes hydrolyzed egg protein, albumin extract, egg yolk extract, egg shell membrane extract, and lecithin, and a cosmetic composition for whitening, wrinkle improvement, and moisturizing is provided, which is prepared by emulsifying small liposome liquid crystals.

Epidermal growth factor, one of the active ingredients of the present invention, is RH-oligopeptide-1, and RH-oligopeptide-1 is also called epidermal growth factor or cell regeneration factor and is a receptor on the surface of the skin. It combines with and promotes the growth of new cells. In general, in skin over the age of about 25, RH-oligopeptide-1 decreases, cell regeneration ability decreases, the stratum corneum thickens, and the aging phenomenon of the skin progresses. RH-oligopeptide-1, a component of the epidermal growth factor, can stimulate skin cells and promote cell regeneration and growth. In the present invention, the inclusion of RH-oligopeptide-1 produces effects such as stimulating skin cells, promoting growth, reducing skin elasticity, dullness, and relieving skin troubles.

And, the egg protein component, which is one of the active ingredients of the present invention, includes hydrolyzed egg protein, albumin extract, egg yolk extract, egg shell membrane extract, and lecithin. Sphingomyelin and lecithin components contain phospholipids, the main components of liquid crystal emulsion, and play a major role in the liquid crystal emulsification process.

These egg protein ingredients brighten the overall impression of the skin as the skin is revived by activating skin cells, maintaining fair skin, maintaining optimal moisture balance, and preventing spots, freckles, age spots, scars, and wounds. It is effective in regenerating marks, not only gives elasticity to the skin, but also makes the skin texture shiny.

Preferably, RH-oligopeptide-1 may be included in an amount of 0.0000001 to 0.0005% by weight based on the total weight of the cosmetic composition, but is not limited thereto.

Preferably, the hydrolyzed egg protein is 0.00001 to 0.05% by weight, the albumin extract is 0.00001 to 0.05% by weight, the egg yolk extract is 0.00001 to 0.05% by weight, the egg shell membrane extract is 0.00001 to 0.05% by weight, and lecithin. It may be included in an amount of 0.00001 to 0.05% by weight, but is not limited thereto.

The cosmetic composition may further include sorbitan stearate, cetaaryl alcohol, burylene glycol, niacinamide, glycol stearate SE, triethyl citrate, and betaine.

The liquid crystal emulsification base component used in the cosmetic composition of the present invention is explained as follows. The lecithin used in the present invention includes both lecithin obtained from soybeans, egg yolks, plant seeds, etc., and hydrogenated lecithin obtained by hydrogenating and saturated lecithin. Ceramides include sphingosine and its derivatives and cerebroside, which are synthetic, fermented, and naturally derived from yeast, animals, and plants.

The liquid crystal emulsification method has the advantages of a non-sticky, soft feel, excellent moisture transfer, long-lasting moisturizing, and deep penetration of effective substances, while the small liposome method is a process that increases skin absorption speed by more than 100 times, making it bio-friendly and multi-liquid crystal. The membrane capsule method can stably capture effective substances such as EGF, egg yolk, and ceramide, and has the advantage of slowly releasing the effective substances.

“Small liposomes” as used herein are colloidal particles in which particles composed of amphipathic molecules with a water-soluble head and an insoluble tail are combined and aligned, and have a double membrane structure similar to a cell membrane. Small liposomes can be expected to restore damaged skin barriers, strengthen skin barriers, and relieve skin irritation. Preferably, the average particle diameter of the small liposome is 1um. If the average particle diameter of the small liposome exceeds 1um, skin penetration and formulation stability may be reduced.

The cosmetic manufacturing method according to the present invention is as follows.

Among the above ingredients, purified water in which the thickener is dispersed and the oil phase are mixed at 70 to 90°C to create a gel phase, and the water phase is mixed at the same temperature, stirred slowly, and then stirred in the process of cooling to 30 to 50°C. A multi-liquid crystal liposome emulsified cosmetic composition can be prepared by adding cosmetic active ingredients, cooling to room temperature, and then defoaming.

For the oil phase, stearates, a liquid crystal forming emulsifier, and sorbitan stearate, etc. are used as an auxiliary emulsifier to strengthen the inside and outside of the liquid crystallized multi-phase liposome. In addition, higher alcohols, higher fatty acids, and fatty acid glycerides are used as reinforcements for liquid crystal liposomes. As a thickener, polyglyceryl methacrylate/propylene glycol mixture, xantangum, or carboxy vinyl polymer can be selected and used.

The water part uses at least one selected from butylene glycol, glycerin, and polyethylene glycol as a moisturizing agent, and macadamia seed oil, etc., is used as an agent to improve the feeling of use.

It should be noted that depending on the cooling time and stirring conditions, especially the stirring conditions after mixing of the water phase, the hardness, properties, and feeling of use of the cosmetic may vary, and the size of the particles when observed under a microscope may vary.

In the present invention, the initial mixture is slowly stirred at 40 to 60 rpm, but after mixing with the water phase, it is preferably stirred at 2500 to 4200 rpm for about 2 to 5 minutes when cooled to a temperature of around 40°C.

If the cooling temperature after mixing the water phase is too high, the particle distribution may become dense and the multi-phase structure may be broken, and if it is too low, many irregular emulsified particles will be generated, causing problems with emulsion stability.

In addition, if stirring is done at less than 2500 rpm, the particle size of the multi-liquid crystal liposomes becomes too large, which may cause cracks in the emulsified particles, and if stirring is done faster than 4200 rpm, the particle size becomes too small and does not have a multi-phase form, which is not good.

The manufacturing process is characterized by adding purified water containing a certain amount of thickener to the oil phase to create a gel phase. At this time, the conditions for preliminary emulsification of the oil phase to create the gel phase are important. If the gel phase is not sufficiently created with the liquid crystal base during the preliminary emulsification, this process This is undesirable because phase transition does not occur during emulsification and there is a risk of phase separation.

In addition, the mixing ratio of non-ionic surfactants such as glycol stearate SE and sorbitan stearate in the oil phase is an important variable in multi-liquid crystal liposome emulsification, so attention must be paid to the composition ratio. If the amount used exceeds 10% by weight of the total weight, it does not match the mixing ratio of the oil phase, so liquid crystal liposomes are formed very small, and if it is less than 0.5% by weight, the liquid crystal film may become thin and the emulsified particles may break.

In addition to the manufacturing method described above, as another method of emulsifying multiple liquid crystal liposomes, the overall composition is the same as the first method and the same proportions and ingredients, except that the purified water in the water phase, excluding the minimum amount of purified water that will dissolve the preservatives and moisturizers, is kept at room temperature. It is a way to put something in. That is, purified water in which the thickener is dispersed and the oil phase are mixed at 70 to 90°C to create a gel phase, and then the aqueous phase containing the thickener and 10 to 20% by weight of purified water is mixed at the same temperature and stirred, This is stirred while cooling to 30 to 50°C, cosmetic active ingredients and the remaining purified water at room temperature are added and stirred slowly, and then cooled to room temperature to 30°C and defoamed to prepare a multi-liquid liposome emulsified cosmetic composition. In this method, the role of each process or ingredient used, ingredient ratio, and process conditions are the same as the above method.

In the cosmetic composition of the present invention, the epidermal growth factor component and egg protein component are stabilized by being encapsulated in liquid crystal emulsified small liposomes and can be continuously released.

In addition, the skin absorption speed and absorption rate can be increased by the above-described small liposome liquid crystal emulsification.

According to another aspect of the present invention, a multifunctional cosmetic for whitening, wrinkle improvement and moisturizing in a serum or cream formulation containing the cosmetic composition is provided.

The ingredients included in the cosmetic composition of the present invention are active ingredients and include ingredients commonly used in cosmetic compositions in addition to the above-mentioned active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, conventional auxiliaries such as pigments and fragrances, And may include a carrier.

The multifunctional cosmetic of the present invention can be prepared in any formulation commonly prepared in the art, for example, serum, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant. - It can be formulated as containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc., but is not limited to this. In addition, it can be manufactured in the form of softening lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

When the formulation of the multifunctional cosmetic of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier ingredients. etc. can be used.

When the formulation of the multifunctional cosmetic of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, in the case of a spray, chlorochloroquine may be used as a carrier ingredient.

It may also contain propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the multifunctional cosmetic of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or sorbitan fatty acid ester can be used.

When the formulation of the multifunctional cosmetic of the present invention is a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, and a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. Agents such as microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the multifunctional cosmetic of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, and sarcosylate. Nate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

The cosmetic composition according to the present invention has a high skin absorption rate by applying a liquid crystal structure to small liposomes that are resistant to external environmental changes and can retain moisture and effective substances for a long time, and have excellent performance by suppressing the release of moisture and effective substances as much as possible. In addition to the moisturizing effect, the continuous release of effective substances can provide a long-lasting makeup effect. In addition, the inclusion of epidermal growth factor and egg protein promotes skin cell regeneration and growth, thereby improving wrinkles and whitening effects.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, these examples are only for illustrating the present invention, and the scope of the present invention should not be construed as being limited by these examples.

Example 1

Among the ingredients in Table 1 below, purified water in which the thickener is dispersed and the oil phase are mixed at 70 to 90°C to create a gel phase, and the aqueous phase is mixed at the same temperature, stirred slowly, and then stirred in the process of cooling to 30 to 50°C. , the active ingredient was added here, cooled to room temperature, and then defoamed to prepare a small liposome liquid crystal emulsified cosmetic composition. The above water part used one or more types selected from butylene glycol, glycerin, and polyethylene glycol as a moisturizing agent, and macadamia seed oil and the like were used as an agent to improve the feeling of use.

The initial mixture is stirred slowly at 40 to 60 rpm, but after mixing with the water phase, preferably when cooled to a temperature of around 40°C, it is stirred at 2500 to 4200 rpm for about 2 to 5 minutes using a homomixer. did. The remaining ingredients were added and mixed using a paddle mixer at a speed of 50 rpm, cooled to 30°C, and then defoamed to prepare a small liposome liquid crystal emulsified cosmetic.

ingredient % (w/w) Purified water To. 100 glycerin 10 Cetearyl alcohol 3 Butylene glycol 3 Coco-Caprylate/Caprate 2.5 Dipropylene glycol 2.5 Neopentyl Glycol Dicaprate 2.5 Caprylic/Capric Triglyceride 2.5 Cetyl Ethyl Hexanoate 2.5 Niacinamide 2.0000 Glycol Stearate SE 2 Triethyl citrate 2 Macadamia seed oil 2 Betaine 2 RH-Oligopeptide-1 0.0002 Hydrolyzed Egg Protein 0.02 Albumin extract 0.02 Egg yolk extract 0.02 Egg shell membrane extract 0.02 lecithin 0.02 Hydrogenated Lecithin 0.75 Stearic Acid 0.75 cetyl palmitate 0.75 Sorbitan Palmitate 0.75 Sorbitan Olivate 0.75 Sorbitan Stearate 0.75 Glyceryl Stearate SE 0.75 Polysorbate 20 0.50 Propanediol 0.50 carbomer 0.1 xanthan gum 0.08 Ceramide NP 0.08 Sodium Hyaluronate 0.08 Sodium Ascorbyl Phosphate 0.08 Adenosine 0.08 Tromethamine 0.08 Caprylic Hydroxamic Acid 0.08 caprylyl glycol 0.08 Ethylhexylglycerin 0.08 1,2-hexanediol One Disodium EDTA 0.05 Spices QS

Example 2

Among the ingredients in Table 2 below, purified water in which the thickener is dispersed and the oil phase are mixed at 70 to 90°C to create a gel phase, and the aqueous phase is mixed at the same temperature, stirred slowly, and then stirred in the process of cooling to 30 to 50°C. , the active ingredient was added here, cooled to room temperature, and then defoamed to prepare a small liposome liquid crystal emulsified cosmetic composition. The above water part used one or more types selected from butylene glycol, glycerin, and polyethylene glycol as a moisturizing agent.

The initial mixture is slowly stirred at 40 to 60 rpm, but after mixing with the water phase, preferably when cooled to a temperature of around 40°C, it is stirred at 2500 to 4200 rpm for about 2 to 5 minutes using a homomixer. do.

The remaining ingredients were added and mixed using a paddle mixer at a speed of 50 rpm, cooled to 30°C, and then defoamed to prepare a small liposome liquid crystal emulsified cosmetic.

ingredient % (w/w) Purified water To. 100 glycerin 10 Butylene glycol 5 Dipropylene glycol 5 Meadowfoam seed oil 1.5 Niacinamide 2 Triethyl citrate One Betaine One vinyl dimethicone One Cetearyl alcohol One Polypropylsilsesquioxane One RH-Oligopeptide-1 0.0002 Hydrolyzed egg protein 0.02 Albumin extract 0.02 Egg yolk extract 0.02 Egg shell membrane extract 0.02 lecithin 0.02 Hydrogenated Lecithin 0.75 octyldodecanol 0.02 Cetearyl olivate 0.2 Sorbitan Olivate 0.2 Glyceryl Glucoside 0.2 Polysorbate 20 0.2 Propanediol 0.2 Ammonium Acryloyl Dimethyl Taurate/VPicopolymer 0.2 Sodium acrylate copolymer 0.2 Sodium polyacrylate 0.08 Ceramide NP 0.1 Sodium Hyaluronate 0.1 Sodium Ascorbyl Phosphate 0.04 Adenosine 0.2 silica 0.08 Caprylic Hydroxamic Acid 0.08 caprylyl glycol 0.08 Ethylhexylglycerin 0.08 1,2-hexanediol One Disodium EDTA 0.05 Spices QS

Test Example 1: Moisture retention test

The small liposome cosmetic prepared in Example 1 (hereinafter referred to as ‘Example cosmetic’) and the cosmetic prepared as a comparative example (hereinafter referred to as ‘Comparative example cosmetic’ / Among the ingredients in Table 1, RH-oligopeptide-1 component , hydrolyzed egg protein, albumin extract, egg yolk extract, egg shell membrane extract, and lecithin, but prepared only by simple liposome emulsification) were measured as follows.

Fifteen women aged 25 to 45 were randomly recruited and a black test was performed. At this time, the measurement was repeated 5 times, and the average value of each individual's moisture retention ability over time was calculated and shown in Table 3.

0.2 cc of each sample was aliquoted using a syringe, applied tightly on a pre-marked grid, and a 10 cm area above the subject's wrist was measured, using Moisture Checker HAD-200.

Early (%) 1 hours 2 hours 3 hours 4 hours 5 hours Example cosmetics 72.1 70.4 67.1 64.4 63.5 63 Comparative example cosmetics 73.5 67.1 58.4 56.7 55.1 54.4

The average value of each individual's moisture retention ability over time is shown in Table 3, and it was found that the moisture retention ability of the example cosmetics according to the present invention was higher than that of the comparative example cosmetics.

Test Example 2: Wrinkle improvement effect test

The wrinkle improvement effect of the Example cosmetics and the Comparative Example cosmetics was evaluated through use tests.

Thirty-two women aged 30 to 45 were randomly recruited and were asked to use the example cosmetic product on one side of the face and the comparative example cosmetic product on the other side of the face. The wrinkle improvement effect after 7 weeks was examined by an expert's naked eye. The degree of wrinkle improvement was evaluated using the evaluation, and the wrinkle improvement effect results are shown in Table 4 below.

Great middle lowness Example cosmetics 16 10 6 Comparative example cosmetics 8 13 11

As shown in Table 4, the cosmetics of Examples of the present invention had an excellent wrinkle improvement effect compared to the cosmetics of Comparative Examples.

Test Example 3: Tyrosinase activity inhibition effect test (whitening effect)

The following experiment was conducted to determine the effect on inhibiting the activity of tyrosinase, an enzyme of tyrosine.

Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. The substrate, tyrosine, was dissolved in 0.05M sodium phosphate buffer solution (pH 6.9) to make a 0.1 mg/ml solution.

The Example cosmetics and Comparative Example cosmetics were used after being dissolved in a buffer solution adjusted to an appropriate concentration.

To determine the effect of inhibiting tyrosinase activity, 0.5 ml of sample tyrosine solution was placed in a test tube and reacted at 37°C for 10 minutes (the control group contained 0.5 ml of buffer solution). The test tube containing this reaction solution was rapidly cooled to stop the reaction, and the absorbance at a wavelength of 475 nm was measured using a spectrophotometer.

The effect of inhibiting the activity of tyrosinase at each concentration of the example cosmetics and the comparative example cosmetics was obtained using the formula below <Equation 1>, and the results are shown in Table 5 below.

[Equation 1]

Tyrosinase activity inhibition rate (%) = 100 - (absorbance at each concentration/absorbance of control × 100)

Concentration (㎍/ml) 10 20 50 100 200 Tyrosinase activity inhibition effect (%) Example cosmetics 8.7 12.1 27.7 31.3 52.9 Comparative example cosmetics 6.2 7.9 10.6 15.9 24.9

As shown in Table 5, the example cosmetics had an excellent tyrosinase activity inhibition effect rate, while the comparative example cosmetics showed a lower tyrosinase activity inhibition rate compared to the example cosmetics.

As shown in the results of the above experimental examples, the cosmetic composition according to the present invention was found to have a high skin absorption rate by applying a liquid crystal structure to a small liposome that is resistant to external environmental changes and can retain moisture and effective substances for a long time. , In addition, it was found that the inclusion of epidermal growth factor ingredients and egg protein ingredients promotes skin cell regeneration and growth, thereby improving wrinkles and whitening effects. Skin irritation did not appear in all subjects who applied the example cosmetics to their skin. didn't

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

A cosmetic composition containing an epidermal growth factor (EGF) component and an egg protein component,
The epidermal growth factor includes RH-oligopeptide-1,
The egg protein component includes hydrolyzed egg protein, albumin extract, egg yolk extract, egg shell membrane extract, and lecithin,
Small liposome liquid crystals are prepared by emulsifying, where:
The epidermal growth factor component and egg protein component are stabilized by being encapsulated in small liposomes and are continuously released.
The skin absorption speed and absorption rate are increased by the small liposome liquid crystal emulsification, and
RH-oligopeptide-1, an epidermal growth factor component, stimulates skin cells and promotes cell regeneration and growth.
wherein it further contains sorbitan stearate, cetaryl alcohol, burylene glycol, niacinamide, glycol stearate SE, triethyl citrate and betaine,
Cosmetic composition for whitening, wrinkle improvement and moisturizing.
According to paragraph 1,
The RH-oligopeptide-1 is contained in an amount of 0.0000001 to 0.0005% by weight based on the total weight of the cosmetic composition, a cosmetic composition for whitening, wrinkle improvement and moisturizing.
According to paragraph 1,
The hydrolyzed egg protein is 0.00001 to 0.05% by weight, albumin extract is 0.00001 to 0.05% by weight, egg yolk extract is 0.00001 to 0.05% by weight, egg shell membrane extract is 0.00001 to 0.05% by weight, and lecithin is 0.00001% based on the total weight of the cosmetic composition. A cosmetic composition for whitening, wrinkle improvement and moisturizing, contained in an amount of from 0.05% by weight.
delete A multifunctional cosmetic for whitening, wrinkle improvement and moisturizing in a serum or cream formulation containing the composition of any one of claims 1 to 3.