KR20100042182A - A anti-aging cosmetic composition containing hydrolysis collagen peptide stabilized in nano-liposome and vitamin c - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a nanoliposome and vitamin C for preventing skin aging is provided to promote collagen synthesis in ECM(extra cellular matrix) and to prevent skin aging with enhanced elasticity. CONSTITUTION: A cosmetic composition for preventing skin aging contains nanoliposome and vitamin C. The nanoliposome is contained in 0.1-50.0 weight% based on total weight. The nanoliposome contains 0.1-20.0 weight% of collagen peptide. The average particle diameter of the nano liposome is 30-70 nm. The molecular weight of the collagen peptide is 50-1000. The cosmetic composition is used in the form of essence, lotion, cream, pack, gel, patch and spray.

Description

A anti-aging cosmetic composition containing Hydrolysis Collagen Peptide stabilized in nano-liposome and Vitamin C}

The present invention relates to a cosmetic composition for preventing skin aging containing nanoliposomes and vitamin C stabilized hydrolyzed collagen peptide, and more specifically, collagen is enzymatically treated to produce low molecular weight hydrolyzed collagen peptide and supply it to the skin. Improves skin's moisturizing ability and promotes collagen synthesis in ECM (Extra celluar matrix) to improve skin wrinkles and improve elasticity of skin to prevent skin aging. In addition, vitamin C is supplied transdermally to the stratum corneum, effectively preventing skin aging by synergistic effects such as skin gloss, skin color improvement, wrinkle reduction, skin elasticity increase. Furthermore, since the hydrolyzed collagen peptide of the present invention is contained by nanoliposomes, the present invention relates to a cosmetic composition for preventing skin aging, which greatly increases the skin penetration effect and maximizes the effect of the active ingredient, and also greatly improves the stability of the formulation.

Skin, the largest organ that makes up the human body, ages rapidly or is exposed to external stimuli, which damages the skin and decreases physiology. This aging of the skin inevitably occurs with age, but the factors causing skin aging due to various environmental factors are increasing, and the demand for quality of life is increasing due to improved living standards. Measures are urgently needed.

The most prominent change in the appearance of the skin accompanying aging is wrinkles. Originally, wrinkles caused by aging may be defined as bones on the surface of the skin due to the loss of collagen and elastic fibers in the dermis. Skin wrinkles are determined by collagen collagen fibers present in the dermal layer. Collagen molecules are produced in fibroblasts, secreted into the dermal layer, and then formed into collagen fibers by autoassociation ( Implant dentistry . 2002. 11 (3): 280 -285). Collagen fibers are involved in the skin's mechanical firmness, tissue adhesion, cell adhesion and cell differentiation in the dermal dermal layer ( Journal of the American Academy of Dermatology , 2001, 17 (4): 610-613). When collagen decreases in production or continuously accumulates due to internal and external factors, it causes wrinkles ( Clinics in geriatric medicine , 2001, 17 (4): 617-630).

In the past, when the synthesis of collagen in vivo becomes active, it is found that the dermal matrix component is increased, and thus it can be expected that the effects of wound healing, increased elasticity, wrinkle improvement, etc. have been used in many products. In particular, peptides for which skin regeneration has been found are known from French Patent No. 2,668,265, US Patent No. 4,665,054, WO 91/3488, WO 91/7431, and the like. However, these peptides have a disadvantage in that precipitation is formed and the stability of the product is greatly reduced.

Retinoic acid, TGF (transforming growth factor, carcinogenesis factor; Cardinale G. et al., Adv. Enzymol., 41, p. 425, 1974), collagen synthesis promoter Proteins (JP8-231370), betulinic acid (betulinic acid (JP8-208424)), chlorella extracts (JP9-40523, JP10-36283, fibroblast proliferation, etc.) are known. However, retinoic acid is unstable, and there is a limit to the amount of use due to stability problems such as irritation and redness when applying the skin. Chlorella extract and the like have little effect, so the collagen synthesis of the skin cannot be substantially expected to improve skin function. Therefore, there is an urgent need for the development of new collagen synthesis promoters which are safe for living bodies and are more effective than conventional collagen synthesis ability promoting substances.

The present inventors have diligently researched to develop an effective anti-aging cosmetic composition using collagen peptides. As a result, less than 1000 low molecular weight hydrolyzed collagen peptides have better skin absorption than conventional collagen (molecular weight of about 300,000). , It promotes collagen synthesis in ECM, improves skin moisturizing effect, and shows that synergistic effect of inhibiting skin wrinkle formation by vitamin C promotes collagen biosynthesis of triple helix structure It became. Furthermore, nanoliposomalized collagen peptides significantly increase the skin penetration effect, remain stable in cosmetic compositions, and relieve complex skin aging phenomena such as wrinkles, decreased elasticity and loss of moisturizing ability during skin application. It confirmed that there was an prevention effect, and completed this invention.

Accordingly, an object of the present invention to provide a cosmetic composition for preventing skin aging comprising nanoliposomes and vitamin C stabilized hydrolyzed collagen peptide as an active ingredient.

The present invention has been made to solve the above-mentioned problem and includes the following means.

Cosmetic composition for preventing skin aging according to an embodiment of the present invention is characterized in that it contains a nanoliposome and vitamin C stabilized hydrolyzed collagen peptide as an active ingredient.

The cosmetic composition for preventing skin aging according to another embodiment of the present invention is characterized in that the nanoliposomes stabilized with the hydrolyzed collagen peptide are contained in an amount of 0.1 to 50.0% by weight based on the total weight of the cosmetic composition.

According to another embodiment of the present invention, the average particle diameter of the nanoliposomes is characterized in that 30-70 nm.

According to another embodiment of the present invention, the hydrolyzed collagen peptide is characterized in that it contains 0.1 to 20.0% by weight based on the total weight of the nanoliposomes.

According to another embodiment of the present invention, the hydrolyzed collagen peptide has a molecular weight of 50 to 1000, characterized in that formed.

According to another embodiment of the present invention, the hydrolyzed collagen peptide is characterized by i) improving skin wrinkles and skin elasticity by promoting the synthesis of collagen or ii) moisturizing.

The anti-aging cosmetic composition according to another embodiment of the present invention is characterized by having a formulation of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

The cosmetic composition of the present invention improves the skin's moisturizing capacity and promotes collagen synthesis in the ECM (Extra celluar matrix) by enzymatically treating collagen, thereby lowering the molecular weight to produce hydrolyzed collagen peptides having a size of 50 to 1000 and supplying them to the skin. Prevents skin aging by improving wrinkles and enhancing skin elasticity. In addition, vitamin C promotes collagen synthesis in the skin and inhibits degradation, thereby synergistically improving skin wrinkles and improving skin elasticity, thereby effectively preventing skin aging. Furthermore, since the hydrolyzed collagen peptide of the present invention is enclosed by nanoliposomes, the effect of increasing the skin penetration is greatly increased, the effect of the active ingredient is maximized, and the stability of the formulation is also greatly improved.

Hereinafter, the present invention will be described in detail.

The cosmetic composition according to the present invention may include a nanoliposome and vitamin C stabilized hydrolyzed collagen peptide to effectively prevent skin aging by bringing synergistic effect to improve the moisturizing power, improve skin wrinkles and improve skin elasticity.

As used herein, the term “nanoliposomes” refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. Liposomes with encapsulated properties are used as carriers to encapsulate bioactive substances. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposomes is preferably formed at 30-70 nm for improved skin penetration and formulation stability.

The nanoliposomes of the present invention may be formed by a mixture comprising hydrolyzed collagen peptides, polyols, surfactants, phospholipids, fatty acids and water.

The hydrolyzed collagen peptide used in the present invention may be formed with a molecular weight of 50 to 1000 by treating proteolytic enzymes to collagen having a molecular weight of about 300,000, and preferably to easily penetrate the skin. To prepare the raw material of collagen by chopping the dried bovine bone, pork skin, and the like, water is mixed with the raw material of collagen at a ratio of 70:30 to 50:50, and then the pH of the solution of the raw material of collagen is 6.5 to After adjusting to 8.5, 0.1 to 1% by weight of proteolytic enzyme was added to the raw material of the collagen, and the treated collagen solution was filtered after the enzyme treatment at 25 to 50 ° C. to remove foreign substances, followed by vacuum concentration, sterilization and Drying can yield the hydrolyzed collagen peptide.

According to a preferred embodiment of the present invention, the hydrolyzed collagen peptide is 0.1-20.0% by weight, preferably 4.0-12.0% by weight relative to the total weight of nanoliposomes.

The polyol used in the preparation of the nanoliposome of the present invention is not particularly limited, preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isoprene glycol, pentylene glycol, erythritol, Ziitol and sorbitol, most preferably propylene glycol. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkyl phosphates, alkyl lactylates, dialkyl phosphates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidyl choline and 20% or less phosphadidylethanolamine. In the preparation of the nanoliposomes of the present invention, the amount of lecithin is 0.5 to 20% by weight, preferably 2 to 8% by weight based on the total weight of the nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of C _12-22 alkyl chains such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. It includes. The amount used is 0.05-3.0 wt% with respect to the total weight of nanoliposomes, preferably 0.1-1.0 wt%.

Water used for the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 5.0-40% by weight relative to the total weight of nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The nanoliposome system thus prepared has the advantage of maximizing skin penetration effect by dissolving various types of poorly soluble substances and stabilizing unstable substances.

Nanoliposomes stabilized with the hydrolyzed collagen peptide used in the present invention are suitably blended in an amount of 0.1 to 50.0% by weight, preferably 1.0 to 30.0% by weight, based on the total weight of the cosmetic composition. If the content of the nanoliposome stabilized hydrolyzed collagen peptide is less than 0.1% by weight, it shows little efficacy of the component. If it is 50% by weight or more, the increase in effect due to the increase of the content is insignificant, and the composition color is undesirably denatured. The chances are high.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to the hydrolyzed collagen peptide nanoliposomes as active ingredients, and include, for example, conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and perfumes, And carriers.

Vitamin C used in the present invention is known to have ultraviolet (UV) blocking effect, antioxidant effect, skin wrinkle improvement through promoting collagen formation, pigmentation improvement effect, immune system enhancement, etc. have.

The vitamin C is a water-soluble substance, there is a limit to the solubility in the organic environment, such as the skin rather than an aqueous solution. In addition, vitamin C is insoluble in commonly used organic solvents such as glycerin, propylene glycol, various fats, etc., and thus has a limit in transporting vitamin C to the skin. In other words, when vitamin C is not ionized, the pH must be 4.2 or less to easily pass through the skin barrier and maintain a non-ionic state upon absorption.

In addition, the amount of skin accumulation of vitamin C is known to be 20 to 40 times higher when used as an external preparation than oral administration. In the case of external skin preparations used for the purpose of improving the skin's anti-wrinkle effect, antioxidant effect, improving skin wrinkles by promoting collagen formation, improving pigmentation, and strengthening immune system, active substances pass through the stratum corneum of the skin to the epidermis. Because it needs to reach the cells that are present, the rate of transdermal absorption of the active substance should be high. In the case of substances similar to the lipophilic properties of the skin, the distribution coefficient to the skin is known to be good for percutaneous absorption. Vitamin C has a high hydrophilicity, so the distribution to the skin is low, so it is difficult to absorb the percutaneous skin.

As described above, vitamin C has various effects, but many studies have been conducted to improve the safety, stability, and low skin absorption.

Among them, to provide ascorbic acid in the form of collagen-binding peptides, Korean Patent No. 0459679 connects a succinoyl group with an ester bond to a hydroxyl group of carbon 5 or 6 of ascorbic acid, It describes the derivatization by imide bonding, ascorbic acid derivatives such as the above shows a superior efficacy than ascorbic acid, but has the disadvantage that the safety is not sustained.

Therefore, by using the nanoliposome system stabilized with hydrolyzed collagen peptides to improve the safety and stability of vitamin C as a cosmetic and to provide excellent skin penetration, it is possible to solve the skin aging phenomenon by stabilizing vitamin C and maximizing the skin penetration effect. have.

The cosmetic composition of the present invention preferably contains the vitamin C in an amount of 0.001 to 20.0% by weight based on the total cosmetic composition. When the cosmetic composition of the present invention contains less than 0.001 weight, the collagen synthesis promotion, collagen decomposition inhibition and skin aging prevention effect of the cosmetic composition is insignificant, and when added in excess of 20.0% by weight, the synergistic effect due to the over-injection is small. Not economical More preferably, the cosmetic composition of the present invention contains 0.01 to 10.0% by weight of vitamin C, even more preferably 0.1 to 5.0% by weight.

The cosmetic composition according to the present invention prevents skin aging through skin wrinkle improvement, skin elasticity improvement, and moisturizing improvement by promoting collagen synthesis. Such facts are specified in the following examples. Therefore, anti-aging of the skin, which is the use of the present invention, is interpreted to include the use of wrinkle improvement, moisturizing improvement and skin elasticity improvement.

The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the art, for example, softening longevity, astringent longevity, nourishing longevity, eye cream, nourishing cream, massage cream, cleansing cream, cleansing The foam, cleansing water, powder, essence, pack and the like is not particularly limited, and may be prepared according to the formulation or purpose of use of the cosmetic.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident. Compounding amount is shown by weight%.

Preparation Example 1 Preparation of Hydrolyzed Collagen Peptides

After preparing the raw material of collagen by chopping the refrigerated pork skin with a crusher, and mixing the water and the collagen uniformly in a weight ratio of 40: 60, and then adjusting the pH of the collagen solution to 7, the raw material of the collagen After adding 0.5% by weight of proteolytic enzyme and enzymatic treatment at 35 ℃ for 6 hours and measuring the viscosity of collagen, the molecular weight reached about 800. Then, the temperature was heated to 100 ℃ for 30 minutes to inactivate excess enzyme and then cooled to room temperature. Insoluble matters were removed using a 500 mesh sieve. The enzyme-treated collagen extract was centrifuged at 10000 rpm to separate only the supernatant in aqueous solution, filtered using a filter press filter, and then concentrated in vacuo to 40% concentration. The concentrated collagen was sterilized continuously for 0.8 seconds at 121 ° C. using a UHT TYPE sterilizer and then dried using a spray dryer to prepare hydrolyzed collagen peptide.

Preparation Example 2 Preparation of Nanoliposomes Containing Hydrolyzed Collagen

The nanoliposomes were added to phase A in the composition of Table 1 below, wetted uniformly, and then phase C was added and heated and mixed to 70-80 ° C. The solution was passed through a high-pressure homogenizer 2-4 times under conditions of 600-1000 bar and then cooled to obtain nanoliposomes that stabilized hydrolyzed collagen having a particle size of 30-70 nm in diameter.

Prize ingredient Content (% by weight) A Propylene glycol 40 B lecithin 5 cholesterol 2.5 Ceramide 2.5 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 C Hydrolyzed Collagen 10 Potassium hydroxide Quantity Purified water To 100

Preparation of Examples and Comparative Examples

To the cosmetic composition having a composition ratio of the following Table 2, Examples and Comparative Examples 1 to 4 containing the nanoliposomes and vitamin C stabilizing the hydrolyzed collagen were prepared in the following composition.

ingredient Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Content (% by weight) Content (% by weight) Content (% by weight) Content (% by weight) Content (% by weight) Nanoliposomes (Preparation Example 2) 20 20 - - Hydrolyzed Collagen - - - 2.0 - Vitamin C 2.0 - 2.0 - - A Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 Mikey-Crystalline 0.7 0.7 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 Tocopherol Acetate 0.2 0.2 0.2 0.2 0.2 Micromethicone 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 0.05 0.05 Purified water To 100 To 100 To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity Quantity Quantity

Experimental Example 1: Moisturizing Effect

In order to evaluate the moisturizing power of each of the cosmetic preparations prepared in Examples and Comparative Examples 1 to 4, the moisture retention ability and the water evaporation amount were measured as follows on the skin to which each cosmetic was applied.

Experimental Example 1-1: Measurement of Water Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples 1 to 4 was coated with a predetermined amount (0.03 g / 16 cm 2) in the lower half of 40 test subjects, and before application, application 1 The moisture content of the skin after hours and after 2 hours of application was measured, and normal skin without any treatment was used as a control. The measuring device used a moisture content measuring instrument (corneometer CM825, Conrage + Khazaka, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 3 below.

division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Control Skin electrical conductivity before application 48.2 48.1 48.2 48.3 48.1 48.2 Skin electrical conductivity 1 hour after application 91.4 86.7 74.9 80.2 68.3 48.4 Skin electrical conductivity 2 hours after application 83.2 75.4 61.2 69.4 55.1 48.3

Experimental Example 1-2: Measurement of Water Evaporation Amount

After applying tape stripping to the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 5-1, and then use a moisture evaporator (Tewameter TM300, Conrage + Khazaka). Co., Germany Federal Bureau), the water evaporation amount was measured 5 times at 1 minute intervals every 12 hours to obtain an average value. The results are shown in Table 4 below.

division Example (%) Comparative Example 1 (%) Comparative Example 2 (%) Comparative Example 3 (%) Comparative Example 4 (%) Control group (%) Moisture evaporation after 0 hours 100 100 100 100 100 100 Moisture evaporation after 12 hours 70.6 74.4 79.0 79.5 89.2 92.1 Moisture evaporation after 24 hours 50.5 55.1 60.3 61.5 74.9 85.1 Moisture evaporation after 36 hours 33.0 38.9 47.5 47.9 63.1 77.7 Moisture evaporation after 48 hours 21.1 24.6 27.9 29.4 51.5 71.0

As shown in the results of Experimental Examples 1-1 and 1-2, the cosmetics of the examples of the present invention have a higher electrical conductivity of the skin than the cosmetics of Comparative Examples 1 to 4, and exhibit a small amount of water loss. Therefore, it can be seen that the cosmetics containing the nanoliposomes and vitamin C in which the hydrolyzed collagen peptides are stabilized have a very good moisturizing effect.

Experimental Example 2: Skin Elasticity Effect

The skin elasticity enhancing effect of the cosmetic composition containing the nanoliposome and vitamin C stabilized hydrolyzed collagen peptide of the present invention was measured. Fifty healthy women (mean age 37 years) over 20 years old were divided into 5 groups under the conditions of temperature 24-26 ° C and humidity 75, compared with Example 1 in Group A, Comparative Example 1 in Group B, and in Group C. Example 2 was applied to the D group, Comparative Example 3, and the E group, the formulation of Comparative Example 4 was applied twice a day (morning and evening) for 12 weeks, mainly around the eyes, and then the skin elasticity tester (Cutometer MPA580, Conrage). + Skin elasticity was measured using Khazaka, Germany. The test results were described as R8 (R8 (12 weeks) -R8 (0 weeks)) values of the cutometer MPA580, where R8 values indicate the nature of the viscoelasticity, and the results are shown in Table 5 below.

Experimental Formulation Skin elasticity effect Example 0.88 Comparative Example 1 0.78 Comparative Example 2 0.71 Comparative Example 3 0.62 Comparative Example 4 0.30

As shown in the results of Table 5, it was confirmed that the skin elasticity of the examples containing the nanoliposomes and vitamin C stabilized hydrolyzed collagen peptides increased compared to the comparative example using each alone.

Experimental Example 3: Skin Penetration Effect

The skin permeation effect of the nanoliposomes and vitamin C containing the stabilized hydrolyzed collagen peptide of the present invention was measured.

Dermal Equivalent (DE) -human normal fibroblasts 1 X 10 ⁵ cells / mL in a gel structure similar to that of human connective tissue 3 mg / mL: 5 X DEM: 2: 2 sodium bicarbonate and 200 0.05 N sodium hydroxide containing mM Hepes buffer was inoculated in a medium mixed with 7: 2: 1 and incubated at 5CO ₂ , 37 ° C for 7 days. It was placed inside a plate separated from the inside and the outside by a membrane made of a 3 μm porous polycarbonate material, and epidermal Keratinocyte on the surface of Derma Equivalent (DE) where fibroblasts were cultured. ) Inoculated with 1 X 10 ⁵ cell / ㎖ cells and cultured for 7 days in the inner and outer K-SFM medium containing EGF and BPE. Subsequently, discard the medium inside the plate so that the air comes into contact with the surface of the cultured cells, and incubate for 2 weeks in a medium mixed with the same amount of K-SFM containing 10% FBS and DMEM without EGF. Artificial skin with epidermis and dermis was obtained. The experimental and comparative examples of the present invention were applied to the artificial skin tissue of the top layer and analyzed for collagen peptide content after 4 hours of incubation. The test results are shown in Table 6 below.

Amount of Hydrolyzed Collagen Peptides in Medium (μg / ml) Example Comparative Example 1 Comparative Example 3 43.7 39.5 3.9

Experimental Results As shown in Table 6, Example 1 containing the nanoliposomes stabilized hydrolyzed collagen peptide and the vitamin C of the present invention and Comparative Example 1 containing the nanoliposomes stabilized hydrolyzed collagen peptide is a hydrolyzed collagen peptide It was found that the skin penetration effect of 10 times or more was superior to that of Comparative Example 3 used as it is. This is because the average particle size of the nanoliposome of the present invention is very small to 30-70 nm, so it is easy to penetrate the skin, the increase of the skin penetration can maximize the efficacy of the active ingredient.

Experimental Example 4: Stability Test of the Formulation

The stability test for the nanoliposome and vitamin C-containing cosmetics stabilized hydrolyzed collagen peptide of the present invention was carried out by the following method.

To test the stability of the cosmetics, the Examples and Comparative Examples were stored in an opaque glass jar in a constant temperature bath at 45 ° C. for 12 weeks, and also in opaque glass in a fully shaded refrigerator kept at 4 ° C. After storing for 12 weeks in a container, the discoloration and odor, and the degree of separation was measured. The results are summarized in Table 7 below. Product discoloration, odor, and separation were evaluated by classifying into the following six grades: 0: no change; 1: very little separation (discoloration); 2: a little separation (discoloration); 3: A little severe separation (discoloration); 4: severely separated (discolored); And 5: very severe separation (discoloration).

division Formulation Stability Example Comparative Example 1 Comparative Example 3 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0.5 0 0.3 0 2.4 0 Deodorant 0.2 0 0.2 0 1.4 0 detach 0 0 0 0 1.6 0

As can be seen in Table 7, the Examples and Comparative Examples were stable at 45 ° C. with little discoloration, discoloration, or separation, and the collagen hydrolytic peptides were highly stabilized by nanoliposomes, and vitamin C was also in the formulation. It is present in a stable state. However, in Comparative Example 3, which was used directly in the formulation without stabilizing the hydrolyzed collagen peptide, separation and discoloration occurred at 45 ° C., so that not only the hydrolyzed collagen peptide was unstable, but also the hydrolyzed collagen peptide entered the formulation and the formulation itself became unstable. It can be seen.

Hereinafter, based on the results of the above experimental example, a cosmetic composition containing nanoliposomes and vitamin C stabilized hydrolyzed collagen peptide of the present invention is presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in Table 8 below, the flexible makeup was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Nanoliposomes 1.0 Vitamin C 0.5 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2. Nutrients (Milk Lotion)

As shown in Table 9, nutrient lotion was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Nanoliposomes 3.0 Vitamin C 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3. Nutrition Cream

Nutritional cream was prepared according to a conventional method as shown in Table 10 below.

Compounding ingredients Content (% by weight) Nanoliposomes 10.0 Vitamin C 2.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4 Massage Cream

Massage cream was prepared according to a conventional method as shown in Table 11 below.

Compounding ingredients Content (% by weight) Nanoliposomes 1.0 Vitamin C 0.5 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5 Pack

Packs were prepared according to conventional methods as shown in Table 12 below.

Compounding ingredients Content (% by weight) Nanoliposomes 2.0 Vitamin C 1.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (8)

A cosmetic composition for preventing skin aging, comprising nanoliposomes stabilized with hydrolyzed collagen peptide and vitamin C as an active ingredient. The cosmetic composition according to claim 1, wherein the nanoliposomes stabilized with the hydrolyzed collagen peptide are contained in an amount of 0.1 to 50.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 2, wherein the vitamin C is contained in an amount of 0.001 to 20.0 wt% based on the total weight of the composition. The cosmetic composition for preventing skin aging according to claim 2, wherein the hydrolyzed collagen peptide is contained in an amount of 0.1 to 20.0% by weight based on the total weight of the nanoliposomes. The cosmetic composition for preventing skin aging according to claim 2, wherein the average particle diameter of the nanoliposomes is 30-70 nm. The cosmetic composition for preventing skin aging according to claim 2, wherein the hydrolyzed collagen peptide has a molecular weight of 50 to 1000. According to claim 1, wherein the hydrolyzed collagen peptide is i) improve skin wrinkles and skin elasticity by promoting the synthesis of collagen, ii) improve the moisturizing ability, iii) improve the stability of the formulation, or iv) the combination of i) to iii) Cosmetic composition for preventing skin aging, characterized in that exhibits the effect of. The cosmetic composition for preventing skin aging according to claim 1, wherein the cosmetic composition has a formulation of a lotion, an essence, a lotion, a cream, a pack, a gel, an ointment, a patch, or a spray.