KR20100038621A - Cosmetic composition for improvement of atopic dermatitis, reinforcing skin barrier function - Google Patents

Abstract

PURPOSE: A cosmetic composition for treating atopic dermatitis is provided to improve skin barrier and strengthen skin moisturization. CONSTITUTION: A cosmetic composition for treating atopic dermatitis contains 0.01-20.0 weight% of wheat germ oil and 1.0-20.0 weight% of nanoliposomal ceramide. The ceramide is ceramide type 3. A polyol used in nanoliposome is propylene glycol, dipropylene glycol, 1,3-butylene glrycol, glycerin, methylpropanediol, isopreneglycol, pentylene glycol, erythritol, xylitol, or sorbitol. The amount of the polyol is 10-80 weight% based on total weight. The cosmetic composition is used in the form of lotion, essence, cream, pack, gel, ointment, patch, or spray.

Description

Cosmetic composition for improving atopic dermatitis by strengthening skin barrier function {reinforcing skin barrier function}

The present invention relates to a cosmetic composition for improving atopic dermatitis by enhancing skin barrier function, and more particularly, to strengthen skin intercellular lipid by a cosmetic composition containing ceramide stabilized with wheat germ oil and nanoliposomes. The present invention relates to a cosmetic composition having an excellent effect on improving atopic dermatitis by enhancing a function and enhancing skin moisturizing power by a decrease in hard epidermal moisture loss.

Atopy means "out of place" or "strange". In 1925, Coca was born with an allergic reaction to food and inhalable substances, causing skin eczema, asthma and high fever. This tendency has been used since the description of atopy.

Atopic Dermatitis is the most common chronic recurrent eczematous disease seen in more than 10% of children. It is a chronic skin disease that occurs frequently in children but can occur at any age. Atopic dermatitis is often perceived as a ill condition when a young child suffers from a short time and touches the ground, but only about 50% of the patients disappear before age 2, 25% continue into adolescence, and the remaining 25% are adults. It continues and suffers. Symptoms of atopic dermatitis are chronic, characterized by a persistent rash on the skin, which causes severe itching and scratches, creating a vicious cycle that becomes more itchy as the skin of the elephant becomes limp. When scratched with nails, secondary bacterial infections occur and become purulent, less resistant to infection, and are more likely to be associated with complications such as allergies, asthma and allergic rhinitis.

Several external environmental factors that are involved in exacerbation of atopic dermatitis are well known, but the cause and pathogenesis of the disease are not yet known. In general, patients with atopic dermatitis suffer from worsening water retention and loss of skin barrier function compared to the general population, and are further affected by infection with secondary bacterial infections. This causes skin dryness, pruritus, inflammation and erythema.

The stratum corneum of the skin, which plays a pivotal role in the function of the skin barrier, acts as a protective barrier against external stimuli and invasion of foreign substances. It maintains moisture in the skin through intercellular lipids and functions as a skin barrier. . The major components of intercellular lipids are ceramides (40-50%), cholesterol (20-25%) and free fatty acids (20-25%). Decreasing or increasing the concentration of any of the three major lipids results in loss of protective barrier function of the skin. Among them, ceramide is the most important lipid of intercellular lipid, and is well known as a component that prevents water evaporation and maintains water. Reduction of ceramide production due to genetic factors or aging reduces the protective barrier function of the stratum corneum, and therefore it is reported that various dermatological symptoms such as atopic dermatitis and psoriasis symptoms (Fulmer & Kramer, J.). Invest.Drm . , 86: 598-602 (1986); Tupker RA et al., Acta Derm.Vereereol . 70: 1-5 (1990)).

The inventors of the present invention effectively apply ceramide to the intercellular lipid to enhance the skin barrier function and reduce transdermal moisture loss when applying cosmetics containing the composition of the ceramide stabilized with nanoliposomes to atopic dermatitis in which the skin barrier function is significantly reduced. It is confirmed that the skin moisturizing ability can be improved, and when applied together with wheat germ oil, which is very rich in tocopherol and essential fatty acid components, it shows that the skin barrier function is much better through the balance of the intercellular lipid component ratio. The invention was completed.

Therefore, an object of the present invention contains ceramide and wheat germ oil stabilized with nanoliposomes to enhance intercellular lipids of the skin to improve skin barrier function, and to enhance skin moisturization by reducing transdermal moisture loss, atopic dermatitis It is to provide a cosmetic composition having a very excellent improvement effect.

In order to solve the above problems, the cosmetic composition according to the present invention may include the following means.

Cosmetic composition for improving atopic dermatitis by enhancing skin barrier function according to an embodiment of the present invention is characterized by containing wheat germ oil and ceramide in the form of nanoliposomes.

The content of the ceramide in the form of nano liposomes is characterized in that 0.001 to 10% by weight relative to the total weight of the cosmetic composition, the content of wheat germ oil is 0.01-20.0% by weight relative to the total weight of the composition do.

The ceramide is characterized in that 1.0-20.0% by weight based on the total weight of the nano liposomes.

According to another embodiment of the present invention, the cosmetic composition is characterized by (i) the recovery of the damaged skin barrier, (ii) strengthen the skin intercellular lipids, (i) enhance skin moisturizing power, (i) a combination thereof The cosmetic composition is characterized in that it is prepared in the form of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

The present invention contains a nano-liposomal form of ceramide and wheat germ oil to enhance intercellular lipids of the skin to improve the skin barrier function, strengthen the skin moisturizing power by reducing the amount of epidermal moisture loss, the effect of improving atopic dermatitis It provides an excellent cosmetic composition. When applying cosmetics containing nanoliposome-type ceramide to atopic dermatitis, which has a significant drop in skin barrier function, it effectively supplies ceramide to intercellular lipid and strengthens skin barrier function and enhances skin moisturizing ability by decreasing epidermal moisture loss. When applied together with wheat germ oil, which is very rich in tocopherols and essential fatty acids, it shows much better skin barrier function through the balance of the lipid ratio between cells.

Hereinafter, the present invention will be described in detail.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 30-70 nm. When the average particle diameter of the nanoliposomes is less than 20 nm or more than 70 nm, the supply of ceramide effective for skin intercellular lipids is very weak among the technical effects to be achieved in the present invention.

The nanoliposomes of the present invention may be prepared by a variety of mixtures, but are preferably prepared by a mixture comprising ceramides, polyols, oily ingredients, surfactants, phospholipids and water.

The amount of ceramide stabilized with the nanoliposomes of the present invention is 1.0-20.0% by weight, preferably 2.0-10.0% by weight, based on the total weight of nanoliposomes. Ceramide used in the present invention includes all types from Type 1 to Type 8, preferably ceramide Type 3.

The polyol used in the nanoliposome of the present invention is not particularly limited, and preferably, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xylitol and It is selected from the group consisting of sorbitol. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposomes of the present invention may be various oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil; Synthetic synthetic oils; Silicone oils such as dimethicone and cyclomethicone series; Animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine; Cerebroside; cholesterol; Cytosterol; Cholesteryl sulfate; Cytosteryl sulfate; C _10-40 fatty alcohol. The amount used is 1.0-30.0% by weight, preferably 3.0-20.0% by weight based on the total weight of nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidyl choline and 20% or less phosphadidylethanolamine. In the preparation of the nanoliposomes of the present invention, the amount of lecithin is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight, based on the total weight of the nanoliposomes.

Water used for the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 5.0-40% by weight relative to the total weight of nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

Wheat germ oil of the present invention may be extracted by mechanically compressing the malt of wheat, or may be produced using a supercritical carbon dioxide extraction method. Wheat germ oil is purified by removing solids, neutralizing, discoloring, and deodorizing. It contains high amounts of tocopherols and has high antioxidant power. In addition, vitamin A, B1, B2, B3, B6, D and essential fatty acids (vitamin F) In particular, wheat germ oil is known to be very effective for muscle fatigue, sunburn, scarring, blood circulation and ataxia.

Physical and chemical properties, chemical composition and essential fatty acid composition of wheat germ oil are as follows.

Physical / chemical properties

  Color: Amber ~ Dark Brown

  Smell: heavy

  Physical state (25 ℃): liquid

  Saponification value (mg KOH / g): 180 ~ 195

  Iodine Value (g I2 / 100): 115 ~ 140

  Specific gravity (20 ℃): 0.900 ~ 0.925

  Cloud point: <10 ℃

-Chemical composition

  Tocopherol: The highest of vegetable oils up to 2,600 ppm

  Free fatty acid: 6 ~ 10%

  Unchecked cargo: 2 to 5%

Fatty acid composition

  Myristic acid <0.1%, palmitic acid 11-16%, palmitoleic acid <1%, stearic acid 1-6%, oleic acid 12-39%, linoleic acid 30-57%, alpha linolenic acid 2-10% ,

  Arachidic acid <1%, eicosanic acid <0.5%, behenic acid <1%, erucin acid <0.3%

The active ingredient included in the cosmetic composition of the present invention is a nano-liposomal form of ceramide and wheat germ oil, and includes components commonly used in cosmetic compositions in addition to the above active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, and pigments. And conventional containing agents such as perfumes. The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, and may have, for example, a formulation such as supple cosmetics, astringent cosmetics, nutrient cosmetics, nutrition cream, massage cream, essence, pack, and the like. .

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited to these embodiments according to the gist of the present invention to those skilled in the art to which the present invention pertains. Will be self explanatory.

Preparation Example 1 Preparation of Ceramide in Nanoliposome Form

The ceramide in the form of nanoliposomes was added to phase A in the composition of Table 1 below, wetted uniformly, and then phase C was added and heated and mixed to 70-80 ° C. The solution was passed through a high pressure homogenizer 2-4 times under conditions of 600-1000 bar and cooled to obtain a ceramide in the form of nanoliposomes having a diameter of 30-70 nm.

Prize ingredient Content (% by weight) A glycerin 65 B Ceramide 10 cholesterol 2 Caprylic / Capric Triglycerides 5 lecithin 5 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 C Potassium hydroxide Quantity Purified water To 100

Preparation Example 2 Examples and Comparative Examples

The composition comprising the nano-liposomal form of ceramide and wheat germ oil (Example) and the composition comprising only the nano-liposomal form of ceramide (Comparative Example 1), the composition not containing both (Comparative Example 2) Prepared by the composition.

ingredient Example Comparative Example 1 Comparative Example 2 Content (% by weight) Content (% by weight) Content (% by weight) Ceramide in the form of nanoliposomes (Preparation Example 1) 2.0 4.0 - Wheat germ oil 2.0 - - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Microcrystalline Wax 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example 1: Test of resilience after skin barrier injury in hairless mice

Acetone was applied as a method of skin barrier damage. Acetone was added to the embryos of 8-12 week old hairless mice to increase transepidermal water loss (TEWL), and then the samples of Example 2 and Comparative Examples 1 and 2 were applied to an area of 4 cm ² . It was. Afterwards, the epidermal moisture loss was measured after 8 hours, and the recovery of skin barrier function was evaluated by evaluating the extent of TEWL reduction. TEWL was measured with Tewameter ™ 300, an evaporimeter from CK (Courage + Khazaka, Cologne, Germany). The results are shown in Table 3 below to reduce the TEWL based on the amount of epidermal moisture loss in the initial damaged barrier state (100%). At this time, the site | part to which no sample was apply | coated was used as a control.

sample 8 hours (% TEWL reduction) Example 77 Comparative Example 1 61 Comparative Example 2 49 Control 29

From the results of Table 3, the cosmetic composition of the embodiment comprising the nanoliposome form ceramide and wheat germ oil is recovered after skin barrier damage compared to Comparative Example 1 containing only the nanoliposome form ceramide and Comparative Example 2 which do not contain all of them. It was confirmed that the effect is very excellent.

Experimental Example 2: Evaluation of Water Retention Capacity and Water Evaporation Rate in Human Body Application

In order to evaluate the moisturizing power of each cosmetic composition prepared in Examples and Comparative Examples 1 and 2, the moisture retention capacity and the amount of water evaporation were measured as follows on the skin to which each cosmetic was applied.

Experimental Example 2-1: Measurement of Water Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic composition prepared in Examples and Comparative Examples 1 and 2 was coated with a predetermined amount (0.02 g / 4 cm 2) in the lower half of 20 test subjects, and before application, The moisture content of the skin after 1 hour of application, 2 hours after application and 4 hours after application was measured. The measuring device used a moisture content measuring instrument (corneometer CM825, Courage + Khazaka, Cologne, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 4 below.

division Example Comparative Example 1 Comparative Example 2 Skin conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 89 77 72 Skin conductivity after 2 hours of application 81 70 59 Skin electrical conductivity 4 hours after application 74 59 52

Experimental Example 2-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 2-1, and then apply CK (Courage + Khazaka, Cologne, Germany) Using the Tewameter TM 300, the evaporimeter of the company, the average value was determined by measuring the amount of water evaporation 5 times at 1 minute intervals every 12 hours. The results are shown in Table 5 below.

 division Example (%) Comparative Example 1 (%) Comparative Example 2 (%) Moisture evaporation after 0 hours 100.0 100.0 100.0 Moisture evaporation after 12 hours 62.1 70.5 85.1 Moisture evaporation after 24 hours 45.3 55.6 74.3 Moisture evaporation after 36 hours 42.6 49.3 64.1 Moisture evaporation after 48 hours 40.2 46.7 55.0

As shown in the results of Experimental Examples 2-1 and 2-2, the Example cosmetics containing the nanoliposomal form ceramide and wheat germ oil of the present invention compared to the cosmetics of Comparative Examples 1 and 2, the electrical conductivity of the skin Is high and shows low water loss. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example 3. Atopic dermatitis improvement effect

In order to confirm whether the cosmetics of the present invention exhibited an excellent atopic dermatitis improvement effect, three groups of 10 patients (16 men and 15 women) consisting of 30 patients composed of ages 2 to 15 years having symptoms of atopic dermatitis The cosmetics of Examples and Comparative Examples 1 and 2 were applied twice a day for 4 weeks and evaluated. The evaluation items were conducted by panel test through questionnaire, and the scale of panel test was 7 scale (-3: very worse, -2: worse, -1: slightly worse, 0: no change, 1: slightly improved, 2: improved) , 3: very improved), and the results are shown in Table 6.

After 2 weeks of use After 4 weeks of use Example 2.1 2.7 Comparative Example 1 1.6 2.2 Comparative Example 2 0.8 0.6

As shown in Table 6, it was found that the cosmetic composition of the embodiment containing the nanoliposome form of ceramide and wheat germ oil is very excellent in atopic dermatitis improvement and alleviation.

Hereinafter, based on the results of the experimental example, the present invention proposes to form a cosmetic composition for improving atopic dermatitis by enhancing the function of the skin barrier by containing the ceramide and wheat germ oil of the nanoliposome form of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in Table 7 below, the flexible makeup was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Nanoceramide in the form of nanoliposomes 1.0 Wheat germ oil 1.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2: Nutrients (Milk Lotion)

As shown in Table 8, nutritious longevity was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Ceramide in the form of nanoliposomes 2.0 Wheat germ oil 2.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3: Nutrition Cream

As shown in Table 9, nutrition cream was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Ceramide in the form of nanoliposomes 2.0 Wheat germ oil 5.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4: Massage Cream

Massage creams were prepared according to a conventional method as shown in Table 10 below.

Compounding ingredients Content (% by weight) Ceramide in the form of nanoliposomes 2.0 Wheat germ oil 10.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5: Pack

Packs were prepared according to conventional methods as shown in Table 11 below.

Compounding ingredients Content (% by weight) Ceramide in the form of nanoliposomes 1.0 Wheat germ oil 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (6)

Cosmetic composition for improving atopic dermatitis by enhancing the function of skin barrier by containing wheat germ oil and ceramide in the form of nanoliposomes. The cosmetic composition for improving atopic dermatitis according to claim 1, wherein the content of the ceramide in the form of nanoliposomes is 0.001 to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition for improving atopic dermatitis according to claim 1, wherein the amount of wheat germ oil is 0.01-20.0 wt% based on the total weight of the cosmetic composition. The cosmetic composition for improving atopic dermatitis according to claim 2, wherein the ceramide is 1.0-20.0 wt% based on the total weight of the nanoliposomes. 2. The cosmetic composition according to claim 1, wherein the cosmetic composition exhibits the action of (i) restoring damaged skin barrier, (ii) strengthening skin intercellular lipids, (i) enhancing skin moisturizing capacity, and (v) a combination thereof. Cosmetic composition for improving dermatitis. The cosmetic composition of claim 1, wherein the cosmetic composition is prepared in the form of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.