KR20200102061A - Composition for improving acne and trichophytia contating wheat germ extract - Google Patents

Abstract

The present invention relates to a composition for improving acne and trichophytia, containing wheat germ extract as an active ingredient. The wheat germ extract contained in the composition of the present invention has inhibitory activity against P. acne bacteria which are acne bacteria, and T. rubrum, T. mentagrophytes, M. canis, M. gypseum, and T. tonsurans which are trichophytia bacteria.

Description

A composition for improving acne and ringworm containing wheat germ extract as an active ingredient {COMPOSITION FOR IMPROVING ACNE AND TRICHOPHYTIA CONTATING WHEAT GERM EXTRACT}

The present invention relates to a composition for improving acne and ringworm containing wheat germ extract as an active ingredient.

In particular, the wheat germ extract contained in the composition of the present invention has inhibitory activity against P. acne bacteria, which are acne bacteria, and T. rubrum, T. mentagrophytes, M. canis, M. gypseum and T. tonsurans , which are acne bacteria. .

Wheat (Triticum vulgare), along with rice and barley, is one of the world's three major crops, an important food. In Korea, the annual consumption of wheat per capita is 34kg, which is the second largest after rice.

In addition, the annual output of wheat is 689.94 million tons, making it one of the grain crops with the largest cultivation area and production. In the case of Korea, in 2010, 345,000 tons of cultivation area of 5,000 ha are produced annually.

Wheat germ (wheat germ) accounts for 2-3% of wheat grains, and has 23 kinds of highly concentrated nutrients, providing important nutrients for wheat growth. However, wheat germ is industrially discarded during the milling process to produce wheat because it does not affect the waste and bitter taste of the product.

The applicant of the present invention is a company that has received the technology transfer of Korean Patent Registration No. 10-1281920 (method for producing wheat germ extract showing antibacterial efficacy), and is researching and developing functionality that can create high added value from wheat germ.

Wheat germ contains major components called methoxy-substituted benzoquinones such as 2,6-dimethoxy-p-benzoquinone (2,6-DMBQ). The functionality of this ingredient has been shown to have anticancer activity or whitening and anti-aging as a cosmetic raw material, but the functionality against acne and ringworm has not yet been revealed.

Accordingly, the present applicant came up with the present invention by studying the acne and ringworm functionality of wheat germ extract.

As a prior art document, Korean Patent Laid-Open No. 10-2018-0033438 describes skin soothing and inflammatory skin diseases improvement of wheat germ fermented products, and Korean Patent Publication No. 10-2010-0038621 discloses atopy of wheat germ oil. Matters concerning the improvement of sexual dermatitis, Republic of Korea Patent Publication No. 10-1929657 on the improvement of the skin swelling of fermented wheat germ, Republic of Korea Patent Publication No. 10-1929658, the matters on the improvement of skin whitening of fermented wheat germ Each of these is described.

Korean Patent Publication No. 10-1281920 Republic of Korea Patent Publication No. 10-2018-0033438 Republic of Korea Patent Publication No. 10-2010-0038621 Korean Patent Publication No. 10-1929657 Korean Patent Publication No. 10-1929658

An object to be solved of the present invention is to provide a composition containing wheat germ extract as an active ingredient for improving acne and ringworm symptoms.

The present invention is to solve the problem by providing a composition for improving acne and ringworm containing wheat germ extract as an active ingredient.

In the above, the wheat germ extract is characterized by having antibacterial activity against P. acne, T. rubrum, T. mentagrophytes, M. canis, M. gypseum, and T. tonsurans bacteria.

In the above, in order to improve acne, the wheat germ extract is contained 78-86 ug/mL,

In order to improve ringworm, wheat germ extract is characterized by containing more than 5 mg/mL.

The present invention is to solve the problem by providing a cosmetic composition for improving acne and ringworm containing wheat germ extract as an active ingredient.

The composition according to the present invention may be provided as a pharmaceutical composition for improving acne and ringworm, and each composition may also be provided as a cosmetic composition, and prepared as a health functional food using a food pharmaceutically acceptable treatment agent. can do.

In the case of a cosmetic using the composition according to the present invention, the content is 0.0001 to 10.0% by weight, preferably 0.0005 to 10.0% by weight, and most preferably 0.005 to 5.0% by weight, based on the total cosmetic composition. If the content is less than 0.0001% by weight, a clear effect of improving acne and ringworm cannot be expected, and when the content is more than 10.0% by weight, there is no significant increase in the effect due to the increase in content.

In order for the present invention to be used as a pharmaceutical composition, it can be prepared by a method known in the pharmaceutical field, and is mixed with itself or a pharmaceutically acceptable carrier, excipient, diluent, etc. to prepare powders, granules, tablets, and capsules. Or it may be prepared and used in a formulation such as an injection. In addition, they can be administered orally or parenterally.

The effective dosage of the composition according to the present invention may be appropriately selected depending on the degree of absorption of the active ingredient in the body, the rate of water activation and the rate of excretion, the age, sex and condition of the patient, and the severity of the disease to be treated.

The composition according to the present invention may be used in cosmetics or feed for livestock, and in each case may be prepared using treatments or additives allowed in cosmetics or feed.

Formulations such as pills, granules, beverages, tablets, and capsules may be prepared by using the composition according to the present invention, and in this case, a treatment agent may be added to prepare each formulation.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions, and include conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers.

The cosmetic according to the present invention may be prepared in any formulation conventionally prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. It is not limited thereto.

More specifically, it may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

The cosmetic composition of the present invention includes a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, sphingo lipids, polysaccharides, and peptides.

As water-soluble vitamins, any ones that can be blended in cosmetics may be used, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. And their salts (thiamine hydrochloride, sodium ascorbate, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) are also included in the water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme method or a chemical synthesis method.

The oil-soluble vitamin may be any one that can be blended into a cosmetic, but preferably, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-alpha tocopherol, D-alpha tocopherol, D-alpha tocopherol), etc. are mentioned. , Their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, DL-alphatocopherol acetate, DL-alpha tocopherol vitamin nicotinic acid E. DL-pantothenyl alcohol, D-pantotenyl alcohol, pantothenylethylethyl alcohol) Ether, etc.) are also included in the oil-soluble vitamin used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microorganism culture, an enzyme or a chemical synthesis method.

As sphingo lipids, any one may be used as long as it can be blended into a cosmetic, and ceramides, phytosphingosine, sphingoglycolipids and the like are preferably mentioned. Sphingo lipids are usually purified from mammals, fish, shellfish, yeast, plants, etc. by a conventional method, or can be obtained by chemical synthesis.

As the polymeric polysaccharide, any one can be used as long as it can be blended in cosmetics, but preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfuric acid, or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The polymeric peptide may be any one as long as it can be blended into a cosmetic, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are exemplified. The polymeric peptide can be purified and obtained by a conventional method such as a purification method from a culture medium of a microorganism, an enzyme method, or a chemical synthesis method, or it can be used after being purified from natural products such as dermis of pigs and cattle, silk fibers of silkworms.

In the cosmetic of the present invention, in addition to the above essential ingredients, other ingredients that are usually blended in cosmetics may be blended if necessary. Other ingredients that may be treated include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, Blood circulation accelerators, cooling agents, limiting agents, purified water, and the like.

In addition, the blending components that may be treated other than are not limited above, and any of the above components can be blended within a range that does not impair the object and effect of the present invention, but is preferably 0.01 to 5% by weight, more preferably, based on the total weight. It is blended in 0.01 to 3% by weight.

The composition according to the present invention has an improvement effect on acne and ringworm.

The composition according to the present invention has an inhibitory activity against P. acne bacteria, which are acne bacteria.

The composition according to the present invention has an inhibitory activity against T. rubrum, T. mentagrophytes, M. canis, M. gypseum, and T. tonsurans five kinds of tinea.

1 is a diagram showing the inhibitory efficacy of wheat germ extracts against Bacillus T. rubrum by concentration.

The terms or words used in the specification and claims should not be construed as being limited to their usual or dictionary meanings, and the inventor may appropriately define the concept of terms in order to describe his own invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention based on the principle that there is.

Accordingly, the embodiments described in the present specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all the technical spirit of the present invention, and thus various alternatives that can be substituted for them at the time of application It should be understood that there may be equivalents and variations.

Preparation Example 1. Preparation of wheat germ extract

Various extraction methods, such as a solvent extraction method, a steam distillation method, a carbon dioxide supercritical extraction method, a steam distillation method, a microwave process extraction method, and a percolation extraction method, may be used for the extraction method of wheat germ, and preferably, a solvent extraction method may be used. The extraction time may also vary.

Various solvents may be used as an extraction solvent. Extractable solvents include water, ethanol, methanol, fatty oil, glycerin, horse oil, flophyllene glycol, ether, chlorform, petroleum ether, hexane, benzene, methylene chloride, ethyl acetate, acetone, butanol, and isopropanol.

Depending on the design conditions, extraction may be concentrated after extraction, or may be dried and pulverized to be powdered and then extracted. In addition, wheat germ can be extracted immediately, dried and powdered. In addition, processes such as filtration and reflux extraction may be added. In addition, the concentration of the extraction solvent may be adjusted according to the type of solvent.

Various methods such as evaporation concentration and dry concentration may be used for the concentration method, and various drying methods such as a positive drying method, a negative drying method, a freeze drying method, and a hot air drying method may be used for the drying method.

Preparation Example 2. Preparation of wheat germ extract

After grinding wheat germ and adding 25 L of secondary distilled water to 100 g of ground wheat germ, stirring for 1 hour, 1 L of chloroform (CHCl3) solution was added to the solution, re-stirred for 30 minutes, and centrifuged. The solvent layer was recovered through. At this time, the chloroform layer was removed after centrifugation, and the remaining filtrate layer was again extracted with chloroform three times in the same manner. The chloroform layers were collected, washed with distilled water and concentrated at a maximum temperature of 40°C using a vacuum evaporator. The concentrated material (6.2 g) was finally redissolved in chloroform and filtered through a 0.45-µm PTFE filter.

Preparation Example 3. Preparation of wheat germ extract

After grinding wheat germ and adding 2.5 L of secondary distilled water to 100 g of ground wheat germ, stirring for 1 hour, 1 L of methylene chloride (CH2Cl2) solution was added to the solution, re-stirred for 30 minutes, and centrifuged. The solvent layer was recovered through separation. At this time, after centrifugation, the methylene chloride layer was removed, and the remaining filtrate layer was extracted with methylene chloride three times in the same manner. The methylene chloride layers were collected, washed with distilled water, and concentrated at a maximum temperature of 40° C. using a vacuum evaporator. The concentrated material (5.9g) was finally redissolved in methylene chloride and filtered through a 0.45-µm PTFE filter.

Preparation Example 4. Preparation of wheat germ extract

After grinding wheat germ and adding 2.5 L of secondary distilled water to 100 g of pulverized wheat germ, stirring for 1 hour, 1 L of ethyl acetate (CH3COOC2H5) solution was added to the solution, and re-stirred for 30 minutes and centrifuged. The solvent layer was recovered through separation. At this time, after centrifugation, the ethyl acetate layer was removed, and the remaining filtrate layer was extracted with ethyl acetate three times in the same manner. After the ethyl acetate layers were collected, washed with distilled water and concentrated at a maximum temperature of 40°C using a vacuum evaporator. The concentrated material (5.5g) was finally redissolved in ethyl acetate and filtered through a 0.45-µm PTFE filter.

Experimental Example 1. Effect of wheat germ extract to inhibit the growth of acne bacteria

The wheat germ extract samples prepared in Preparation Examples 2 to 4 were used to test the effect of inhibiting the growth of acne bacteria.

1. Experimental process

To evaluate the antimicrobial activity of wheat germ extract and indicator component (2,6-dimethoxy-1.4-benzoquinone, DMBQ) against acne bacteria, it was diluted to a concentration of 1.5 x 10 ⁸ CFU/ml after 48 hours pre-culture in RC liquid medium. 1% of the sample solution diluted with DMSO was added to one RC medium and cultured at 37° C. for 48 hours under anaerobic conditions to confirm the reduction rate (%) of P. acne bacteria.

2. Experiment result

division Preparation Example 2 Extract Preparation Example 3 Extract Preparation Example 4 Extract Index component MIC concentration 86 ug/mL 82ug/mL 78 ug/mL 8 ug/mL

Wheat germ extracts of Preparation Examples 2 to 4 were found to have a minimum inhibitory concentration (MIC) at a concentration of 86 ug/mL, 82 ug/mL, and 78 ug/mL, respectively, and the DMBQ derived as an indicator component was 8 ug/mL. MIC was confirmed at the concentration.

Experimental Example 2. Effect of wheat germ extract on inhibiting the growth of thyme

The wheat germ extract sample prepared in Preparation Example 4 was used to test the effect of the wheat germ extract on inhibiting thyme.

1. Experimental process

In order to evaluate the antibacterial activity of wheat germ extract against Trichomonas, 1% of the sample solution diluted with DMSO was added to the RC medium diluted to a concentration of 1.5 x 10 ⁸ CFU/ml after 48 hours pre-cultivation in RC liquid medium. Incubation under anaerobic conditions for 48 hours was performed to confirm the reduction rate (%) of T. rubrum, T. mentagrophytes, M. canis, M. gypseum, and T. tonsurans 5 species of Tinea bacteria .

2. Experiment result

T. rubrum T. mentagrophytes M. canis M. gypseum T. tonsurans CM (100 ppm) 25.05±0.01 19.82±0.04 12.69±0.01 14.49±0.09 12.39±0.06 CM (500 ppm) 36.89±0.35 25.48±0.37 16.48±0.22 17.67±0.01 13.91±0.06 CM (1000ppm) 42.13±0.01 29.56±0.13 18.71±0.04 23.21±0.27 15.09±0.10 CHCL3 10.65±0.06 9.39±0.01 10.42±0.01 13.08±0.03 12.84±0.01 Wheat germ extract 27.63±0.01 17.80±0.70 10.47±0.01 12.81±0.02 11.15±0.08

(CM: chloramphenicol, positive control, CHCL3: chloroform, negative control, inhibition zone test)

As a result of measuring the antimicrobial activity (disk method, mm) against wheat germ extract, significant inhibitory activity was found against 5 types of thyme. In particular, T. rubrum showed a high activity of 27.63±0.01 mm.

In addition, as a result of measuring MIC against T. rubrum , wheat germ showed high activity of 5.00 mg/mL.

Experimental Example 3. T. rubrum inhibition efficacy of wheat germ extract

1. Experimental process

It was examined the T. rubrum inhibitory effect of each concentration of wheat germ extract prepared in Preparation Example 4.

2. Experiment result

Referring to FIG. 1, it was confirmed that T. rubrum bacteria were killed within 12 hours at a concentration of 5.00 mg/mL or more.

Claims (4)

A composition for improving acne and ringworm containing wheat germ extract as an active ingredient.
The method according to claim 1,
The wheat germ extract P. acne, T. rubrum, T. mentagrophytes, M. canis, M. gypseum, a composition for improving acne and ringworm, characterized in that it has antibacterial activity against the bacteria T. tonsurans .
The method according to claim 1,
To improve acne, wheat germ extract contains 78-86 ug/mL,
In order to improve ringworm, a composition for improving acne and ringworm, characterized in that the wheat germ extract contains 5 mg/mL or more.
A cosmetic composition for improving acne and ringworm containing wheat germ extract as an active ingredient.

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