KR20120060650A - Composition comprising a purified distillate of rice husk showing anti-bacterial and anti-wrinkle activity - Google Patents

Abstract

PURPOSE: A composition containing rice husk distilled/purified liquid is provided to activate anti-oxidation and to suppress collagenase and elastase activation. CONSTITUTION: A cosmetic composition contains rice husk distilled/purified liquid as an active ingredient with antiaging, antioxidation, and antibacterial activity. The liquid is primary distilled/purified liquid, secondary distilled liquid, or tertiary distilled/purified liquid. A method for preparing the liquid comprises: a step of carbonizing rice husk at 200°C-800°C for 10-200 minutes; a step of condensing smoke discharged during carbonization to obtain crude rice bran distilled liquid; a step of decompressing the distilled liquid and distilling at 20°C-90°C to prepare a primary distilled liquid; a step of adding 1-3% of activated charcoal to the distilled liquid; and a step of heating, stirring and purifying.

Description

Composition comprising a purified distillate of rice husk showing anti-bacterial and anti-wrinkle activity

The present invention relates to a skin external pharmaceutical composition and a cosmetic composition containing chaff distilled distillate purified liquid showing anti-aging, antioxidant and antibacterial activity as an active ingredient.

[Document 1] Kang Dae Gap, New Functional Natural Products Exploration and Utilization Technology for Agricultural Products, Development of Ceramic Raw Materials and Industrial Materials using Silicon Silicon of Rice, Korea Atomic Energy Research Institute

[Ref. 2] Lee, Kye-Won and 1 others, Korean Pharmaceutical Society, Vol. 8, No. 2 2008: 87 ~ 93.

[3] Jung Il-sun, 3 others, Journal of Life Science, Vol. 17, No. 1, 2007: 105 ~ 109.

[History 4] Ji Heung-sook, Master's Thesis, Chung-Ang University: 2005.

[Document 5] Kim, Sung-Pil et al., J. Appl. biol. Chem, 52 (4): 174-179

[Document 6] Lee, Kyung-Min et al., Korean Journal of Biotechnology and Bioengineering, 19 (5), 2004: 381 ~ 384.

[Document 7] Jong-Soo Kim, Kyungpook National University Thesis: 2007.

[Reference 8] Choi Seung-Hyun, Chosun University Thesis: 2003.

[Document 9] Cho, Kwang-Yeon, Korea Research Institute of Chemical Technology, 2001

[Document 10] Korean Patent Registration No. 10-931529

Rice husks are the husks of rice, the largest by-product of rice processing, accounting for 20% of the weight of rice. Domestic rice husk production is estimated at 5,961,693 tons of rice in 2007, and about 1.2% tons of rice husk are produced annually, about 20%. Conventionally, it was produced in a small rice mill processing plant and limited in its utilization method, but now a large-scale milling plant is planned to be installed by 2010, and rice grain by-product chaff will be concentrated in the rice mill juksan complex. The efficient use of this rice hull is very important.

Chaff has the proper characteristics as an energy source, but is currently classified as a waste. Chaff is known to be inadequate for compost and livestock feed due to its low internal corrosion and low digestion efficiency because it is heavily coated with silicon. The main components of these rice hulls are 27-30% of soluble carbohydrates, 35-46% crude fiber, 13-21% crude ash, 2-3% crude protein, and 0.3-0.8% crude fat. It is known (Kang Dae Gap, new functional natural material search and utilization technology for agricultural products, development of ceramic raw materials and industrial materials using silicon bodies of rice, Korea Atomic Energy Research Institute).

As chaff has such characteristics, it is classified as a waste because it has no use and proper treatment, and it is one of the important things to make the chaff a reasonable use and use of waste.

Therefore, the present inventors have patented the cosmetic composition using the anti-allergic activity by preparing chaff herb distillate using the chaff as a material (Korean Patent Registration No. 10-931529), and further purified the chaff distillate To determine the new efficacy using their anti-aging, antioxidant and antimicrobial effect.

Accordingly, the present inventors prepared a distilled purified liquid obtained by further purifying chaff herb distillate from the chaff as a material, and then measured anti-aging and electron donating activities such as collagenase inhibitory activity, Elastase inhibitory activity, etc. The present invention was completed by confirming antioxidant and antimicrobial activities such as SOD-like activity measurement and Xanthine oxidase inhibitory activity.

In order to achieve the above object, the present invention provides a skin external pharmaceutical composition containing chaff distilled distillate purified liquid showing an anti-aging, antioxidant and antibacterial activity as an active ingredient.

In addition, the present invention provides a cosmetic composition containing as an active ingredient chaff herb distillation tablets showing anti-aging, antioxidant and antibacterial activity.

Rice husk distillate purified liquid of the present invention includes a "primary distilled purified liquid", "secondary distilled purified liquid" or "tertiary distilled purified liquid".

Chaff herb distillation purification liquid of the present invention, "secondary distillation purification liquid" or "tertiary distillation purification liquid" can be prepared as follows.

As defined herein, “rice husk distillate purified liquid” refers to a chaff, preferably from 200 ° C. to 800 ° C., preferably from 300 ° C. to 600 ° C., for 10 minutes to 200 minutes, preferably 40 minutes to 120 minutes. Carbonized using Daewon GSI Co., Ltd. carbonization product (DCH-600) and condensing the smoke discharged during the carbonization process to obtain crude chaff herb distillate, decompression of the crude chaff herb distillate 20 ℃ ~ A first step of distilling at a low temperature of 90 ° C., preferably 40 ° C. to 70 ° C., to obtain a primary distillate of rice hulls; A second step of adding 1% to 3% of activated carbon to the rice hull distillate, followed by heating and stirring to obtain a secondary distillation purified liquid after filtration of a filter press; An oxide, preferably an oxide such as hydrogen peroxide, is added to the secondary distillation purification liquid obtained in the second step, 1% to 5% (v / v), followed by heating and stirring to obtain a third distillation purification liquid. The rice hull distillate purified liquid obtained through the process including three steps can be obtained.

The present invention provides a skin external pharmaceutical composition containing as an active ingredient chaff herb distilled herbal vinegar showing anti-aging, antioxidant and antibacterial activity.

In addition, the present invention provides a cosmetic composition containing as an active ingredient chaff distilled herbal vinegar showing anti-aging, antioxidant and antibacterial activity.

As defined herein, the group consisting of "Chinese herb distillate herbal liquor", as defined herein, "Chef herb first distillate", "Chugar herb second distillate", or "Chuly herb third distillate" At least one herbal extract selected from the group consisting of perilla leaf, mugwort, gujippong and Chinese quince extract in at least one chaff herb distillate, preferably chaff herb third distillate, preferably perilla leaf and mugwort One or more herbal extracts selected from the group consisting of, more preferably chaff herb distillation purified or herbal extracts are 1: 0.001-0.5 (v / w), preferably 1: 0.001-0.1 (v / w), herbal herb Middle leaf or mugwort vinegar is characterized in that the chaff herb distilled herbal vinegar obtained by mixing 1: 0.001-0.5 (v / w), preferably 1: 0.001-0.1 (v / w).

Hereinafter, the present invention will be described in detail.

For example, the rice hull distillate as defined herein may be used as a charcoal, preferably for 10 to 200 minutes, preferably 40 to 120 minutes, at 200 ° C. to 800 ° C., preferably at 300 ° C. to 600 ° C. Preferably, carbonization is performed using Daewon GSI Co., Ltd. carbonization product (DCH-600) and condensation of the smoke discharged during the carbonization treatment to obtain crude chaff herb distillate, and then the crude chaff herb distillate is decompressed to 20 ° C. A first step of distilling at a low temperature of ˜90 ° C., preferably 40 ° C. to 70 ° C., to obtain a primary distillate of rice hulls; A second step of adding 1% to 3% of activated carbon to the rice hull distillate, followed by heating and stirring to obtain a secondary distillation purified liquid after filtration of a filter press; After the addition of 1% to 5% (v / v) of an oxide, preferably hydrogen peroxide, to the secondary distillation purification liquid obtained in the second step, heating and stirring to obtain a third distillation purification liquid A rice hull distillation purified liquid can be obtained by a manufacturing process comprising the same.

In addition, if necessary, one or more herbal extracts selected from the group consisting of oleander, mugwort, gujippong and Chinese quince extract in the distillation purified liquid obtained in the first, second or third stages, preferably One or more herbal extracts, chaff herb distillates or herbal extracts selected from the group consisting of mugwort vinegar are formulated at a compounding ratio of 1: 0.001-0.5 (v / w), preferably 1: 0.001-0.1 (v / w). The herbaceous or mugwort vinegar in the herbal vinegar is 1: 0.001-0.5 (v / w), preferably 1: 0.001-0.1 (v / w) through the step of adding the chaff herb distilled herbal vinegar of the present invention Can be obtained.

The present inventors confirmed the antioxidant activity assay and collagenase and Elastase activity inhibitory effect on the chaff distilled purified liquid and chaff herb distilled herbal herbal liquors obtained by the above-mentioned method, and the external skin pharmaceutical composition and cosmetic composition exhibiting antioxidant and antimicrobial activity. It was found to be useful for the provision of.

The present invention provides an external skin pharmaceutical composition showing the anti-aging, antioxidant and antimicrobial activity containing the composition obtained by the above production method as an active ingredient.

The skin external pharmaceutical composition exhibiting anti-aging, antioxidant and antimicrobial activity of the present invention comprises 1 to 50% by weight of the composition, based on the total weight of the composition.

The external dermal pharmaceutical composition comprising the composition of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical composition comprising the composition according to the present invention may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, external preparations such as syrups, aerosols, and sterile injectable solutions, respectively, according to conventional methods. It provides a skin external pharmaceutical composition, preferably creams, gels, patches, sprays, ointments, warnings, lotions, linen, pasta or cataplasma. Carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, oligosaccharides, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition showing the antioxidant and antimicrobial activity containing the composition of the present invention is a skin external preparation formulation that can be applied to the skin as a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma It may be prepared and used as a pharmaceutical composition in the form of the external preparation of the skin, but is not limited thereto.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the preferred effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. Administration may be administered once a day or may be divided several times. The dose is not intended to limit the scope of the invention in any way.

The composition of the present invention can be used in various ways, such as cosmetics, face wash and shampoo for medicinal effects on the skin of the present invention. Examples of the product to which the present composition may be added include various cosmetic compositions such as skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, and moisturizing cream. , Hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

The composition of the present invention comprises 0.001 to 90% by weight, preferably 0.001 to 70% by weight, most preferably 0.001 to 50% by weight of the extract relative to the total weight of the cosmetic composition.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention.

Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

Examples of the ester-based oils and fats include tri2-ethylhexaneglyceryl, 2-ethylhexanoate cetyl, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate and butyl stearate Ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristyl, isosteyl myristin, isostearyl palmitate, isostearyl palmitate, octylate acid octylate, isostearic acid isetyl, diethyl sebacate, adipic acid didi Isopropyl, isoalkyl neopentanoate, tri (capryl, capric acid) glyceryl, tri2-ethylhexanoic acid trimethylolpropane, triisostearic acid trimethylolpropane, tetra2-ethylhexanoic acid pentaerythritol, Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristin, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, cesinooleate, Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2- Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid) propylene glycol, dicapryl Acid propylene glycol, dipentane neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitate glyceryl, triisostearic acid glyceryl neopentane dodecyl, Isostearyl octanoate, octyl isononate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Octyl stearate, polyglycerol oleic acid ester, polyglycerin isostearate ester, triisoceyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, cetyl lactate, octyl lactate, triethyl citrate , Acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, diethyl ethyl succinate, diisobutyl adipicate, diisopropyl sebacate, seba Dioctyl Citrate, Cholesterol Stearate, Cholesterol Isostearate, Cholesterol Hydroxystearate, Cholesterol Oleate, Dihydrocholesteryl Oleate, Pitsteryl Isotearate, Pysterol Oleate, 12 -Steloylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-stealoyl Ester system, such as hydroxystearic acid isostearyl, etc. are mentioned.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengal, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

As alcohol, higher alcohols, such as ethyl alcohol, are mentioned.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More Preferably from 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the chaff distillate as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments and fragrances Conventional adjuvants and carriers are included.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

As described above, the composition containing the tertiary purified chaff herb solution of the present invention exhibits anti-oxidant activity and inhibits collagenase and elastase activity, and thus may be used as an external skin pharmaceutical composition and cosmetic composition showing anti-aging, antioxidant and antimicrobial activity. Can be.

1 is a view showing the production process of chaff vinegar and herbal vinegar by the method;
Figure 2 is a diagram showing the DPPH radical inhibitory activity of chaff herb for skin aging prevention,
Figure 3 is a diagram showing the measurement of FRAP antioxidant activity using four kinds of vinegar,
Figure 4 is a diagram showing the measurement of DPPH antioxidant activity using four kinds of vinegar,
5 is a diagram showing the SOD-like activity of chaff herb solution for the prevention of skin aging,
6 is a diagram showing the Xanthine oxidase inhibitory activity of chaff herb for the prevention of skin aging,
7 is a diagram showing the collagenase inhibitory activity of chaff herb solution for the prevention of skin aging,
8 is a diagram showing the Elastase inhibitory activity of chaff herb for the prevention of skin aging,
9 is a strain with high infection rate in atopic dermatitis ( Staphylococcus Aureus ) shows the antimicrobial effect by paper disk method.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Example 1 Preparation of Chaff Vinegar Solution

Rice husk distillate, as defined herein, was prepared using a carbonizer (DCH-600, Daewon GSI), and condensed smoke emitted during carbonization of rice husk (domestic), crude rice husk distillate (hereinafter referred to as “RH After obtaining 1000 l of the crude chaff herb distillate under reduced pressure and distilling at a low temperature of 50 ° C. to obtain 900 l of chaff herb primary distillate (hereinafter referred to as “RH-1”) to obtain activated carbon 1%. To 3% was added, followed by heating and stirring to obtain 850 L of a secondary purified rice hull vinegar (hereinafter referred to as "RH-2") after filtration with a filter press to add 1% to 5% of oxide, followed by heating and Stirring yielded 700 L of tertiary purified rice hull liquor (hereinafter referred to as "RH-3") (see Figure 1).

Example 2. Preparation of Herbal Supernatant

After washing and drying 5 g of Korean ginseng leaf (domestic) and 5 g of mugwort (domestic), and then 100 ml of rice husk herb solution were added at 100 ° C. for 24 hours at the temperature of 85 ° C. 90 ml of mugwort water extract (hereinafter referred to as “SS-WA”) was obtained (see FIG. 1).

Experimental Example 1. Antioxidant Activity Assay

Experiments were carried out for the antioxidant activity assay of the samples obtained in the above examples.

1-1. DPPH radical scavenging activity

DPPH (1,1-diphenyl-2-picrylhydrazyl) is itself a very stable free radical, a purple compound with characteristic light absorption at 517 nm. DPPH is very safe in organic solvents such as alcohol, and because it is discolored by proton-radical scavenger during antioxidant mechanism, antioxidant activity can be easily observed with the naked eye. That is, the activity assay of the sample capable of scavenging free radicals was performed using the DPPH radical scavenging activity method, which is relatively simple and rapid and widely used. That is, add 200 μL of sample and 800 μL of DPPH solution in which DPPH is dissolved in ethanol at a concentration of 100 μM, mix, and leave it in the dark at room temperature for 30 minutes, and then absorbance at 517 nm with UV spectrophotometer (UV-1800, Shimadzu CO., Kyoto, Japan). Measured. And DPPH radical scavenging activity ratio (% inhibition) was calculated as shown in Equation 1 below.

As a positive control, BHA (Butylated hydroxyanisole), which is known to have an antioxidant effect, was used to compare the antioxidant effects of rice hull. Both rice hull extracts showed DPPH radical scavenging activity in a concentration-dependent manner and showed similar inhibitory activity when compared with the positive control group. These results suggest that chaff herb itself has a high antioxidant activity and will be of great help in preventing aging of free radicals that commonly occur in skin (see FIG. 2).

1-2.Ferric ion reducing antioxidant power (FRAP)

FRAP assay was used in the experiment by changing the method of Benzie et al. Acetate buffer (pH 3.6, 23mM) is prepared using C ₂ H ₃ NaO ₂ and acetic acid (C ₂ H ₄ O ₂ ). Since 10mM TPTZ solution was made using 40mM HCl and TPTZ (2,4,6-tripyridyl-s-triazine). The cocktail solution for the experiment was acetate buffer (pH 3.6, 23 mM), 10 mM TPTZ (2,4,6-tripyridyl-s-triazine) and 20 mM FeCl _{3 ?} 6H ₂ O was mixed at a ratio of 10: 1: 1, and then maintained at 37 ° C. until the experiment. 30μL of the sample solution was put into the Cubit cell and then mixed with the cocktail solution 1mL. After standing in the dark for 30 minutes, the absorbance was measured at 593 nm using a Victor3 1420 multiabel counter (PerkinElmer Inc., Boston, MA, USA). Reducing power was expressed as the ferrous sulphate equivalent value (μM) obtained from the simple regression equation of absorbance according to the concentration of ferrous sulphate measured at each experiment.

As a result of the experiment, the purified chaff extract was found to have a relatively low activity compared to other samples as in the DPPH experiment. The vinegar vinegar and mugwort vinegar were found to have a relatively high activity compared to the purified chaff vinegar. All three solutions showed similar high activity as in DPPH (see FIG. 3).

1-3. DPPH Antioxidant Activity Verification

DPPH antioxidant activity was measured using four kinds of vinegar (tablet vinegar vinegar, purified vinegar vinegar, mugwort vinegar solution). DPPH activity is the ability to share electrons, depending on the characteristics of the sample, depending on the degree of sharing of electrons.

The reducing power of the sample was measured by the electron donating effect on 1,1-diphenyl-2-picryl hydrazyl (DPPH) of each sample. That is, 0.5 ml of 0.4mM DPPH solution was added to 1 ml of each sample prepared for each concentration, and the reaction solution was reacted for 30 minutes at 37 ° C. after vortex mixing for 10 seconds, and then the absorbance was measured at 517 nm using a microplate reader.

As a result of the experiment, it was found that the purified chaff extract had the lowest DPPH antioxidant activity compared to the other three samples. It was thought that the chaff herb which had been purified was reduced in activity, whereas the chaff herb showed high antioxidant activity.

It was found that the activity was increased depending on the concentration of the sample, and the activity was about 2-18% according to the concentration of 1-10 μl / well. Purified herb solution showed high activity of 24% for 1μl / well, 71% for 3μl / well, and 82% for 10μl / well It was. The mugwort liquor showed the same activity as the purified liquor, and the activity was increased according to the concentration. The activity was 27% at 1μl / well, 61% at 3μl / well, and 82% at 10μl / well. Root leaf vinegar showed 20% activity at 1 μl / well, 64% at 3 μl / well, and 79% at 10 μl / well (see FIG. 4).

1-4. SOD - like  Active measurement

To 0.2 mL of each sample, 3 ml of Tris-HCl buffer (50 mM tris [hydroxymethyl] aminomethane + 10 mM EDTA) and 0.2 ml of 7.2 mM pyrogallol were added to 0.2 mL of each sample, which was left at 25 ° C for 10 minutes and the reaction was stopped with 1 ml of 1N HCl. Absorbance at 420nm was measured using a microplate reader.

As a result of the experiment, the chaff herb all showed SOD-like activity scavenging effect in a concentration-dependent manner, at 100 ml / ml showed an inhibitory activity close to 100% (see Fig. 5).

1-5. Xanthine oxidase  Measurement of inhibitory activity

0.1 ml of sample solution and 0.2 ml of 0.1 M potassium phosphate buffer at pH 7.5 were added to 0.2 ml of substrate solution dissolved in 2 mM xanthine, 0.2 unit / ml xanthine oxidase 0.1 ml was added and reacted at 37 ° C. for 5 minutes, followed by 1 N HCl 1 ml. After the reaction was terminated, the absorbance of the uric acid generated in the reaction solution at 292nm was measured. Xanthin oxidase inhibitory activity was shown by the absorbance decrease rate of the sample solution addition group and no addition group.

As a result of the experiment, Xanthine oxidase is an enzyme that produces superoxide radical in the process of producing uric acid using xanthine as a substrate. Inhibition of superoxide anion by enzymes of xanthine / xanthine oxidase is shown by superoxide anion scavenging and xanthine oxidase enzyme inhibition. BHA was used as a positive control to compare the superoxide radical scavenging effect of chaff extract. As a result, the chaff herb showed a superoxide radical scavenging effect in a concentration-dependent manner, and showed a similar inhibitory activity compared to the positive control group, showing an excellent superoxide radical scavenging effect (see FIG. 6).

Experimental Example  2. Determination of skin anti-aging activity

In order to measure the skin anti-aging activity of the sample obtained in the above example, the experiment was conducted by applying the method of the following literature (Lee Kye-won et al., Korea Pharmaceutical Association, Vol. 8 No. 2 2008: 87 ~ 93.; , 3 others, Journal of Life Science, Vol. 17, No. 1, 2007: 105 ~ 109.; Ji Heung-sook, Chung-Ang University (Thesis): 2005.; Sungpil Kim et al., 1 J.Appl. (4): 174-179).

2-1. Collagenase  Measurement of inhibitory activity

In order to confirm the skin aging inhibitory effect was measured collagenase inhibitory activity as follows. In other words, 4 mM CaCl ₂ was added to 0.1 M Tris-HCI buffer (pH 7.5), and 0.25 ml of substrate solution containing 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg (0.3 mg / ml) and sample solution 0.1 0.15ml of collagenase (0.2mg / ml) was added to the mixture, and the mixture was left at room temperature for 20 minutes, and then 0.5ml of 6% citric acid was added to stop the reaction. Then, 1.5ml of ethylacetate was added to measure the absorbance at 320nm. It was. Collagenase inhibitory activity was shown by the absorbance reduction rate of the sample solution added and the no added solution.

As a result of the experiment, the chaff herb showed a collagenase inhibitory activity of 40 ~ 50% at 50ml / ml concentration, 100% collagenase inhibitory activity at 100ml / ml concentration. In addition, the inhibitory activity was high in a concentration-dependent manner. In particular, it showed a high inhibitory activity close to epigallocatechin gallate (EGCG), a single substance derived from green tea extract, which was used as a control group. Chaff extract was found to have a high inhibitory rate of collagenase in a concentration-dependent manner, and is expected to improve the wrinkles of the skin by preventing degradation of collagen (see FIG. 7).

2-2. Elastase  Measurement of inhibitory activity

To determine the effect of improving skin wrinkles, porcine pancreas elastase inhibitory activity was measured using N -succinyl- (L-Ala) ₃ - p -nitoanilide as a substrate and the production of p -nitoanilide for 30 minutes at 37 ℃. Each test solution was prepared to a certain concentration, 0.1ml each was added to the test tube, and elastase, pancreatic solution, and Type I: From Porcine Pancreas (0.6units / ml) dissolved in 50mM Tris-HCl buffer (pH 8.6) were added. N- succinyl- (L-Ala) ₃ - p -nitoanilide (1mg / ml) dissolved in 50mM Tris-HCl buffer (pH 8.6) was added as a substrate and reacted for 30 minutes using a microplate reader. Measured. Elastase inhibitory activity was expressed as the absorbance decrease rate of the sample solution added and the no added solution.

As a result of elastase inhibitory activity using chaff extract, mugwort vinegar showed 75% inhibitory activity at the concentration of 100ml / ml and secondary purified vinegar showed 50% inhibitory activity. At low concentrations, no inhibitory activity was observed, indicating no concentration-dependent inhibitory activity. It showed a high inhibitory activity at a concentration of 100ml / ml, it can be seen that the elastase inhibitory activity in the vinegar vinegar solution from the above results (see Fig. 8).

Experimental Example  3. against antibacterial data

In order to measure the antimicrobial activity of the sample obtained in the above example, the experiment was conducted by applying the method of the following literature (Kyungmin Lee et al., Korea Biotechnology Journal, 19 (5), 2004: 381 ~ 384 .; Kyungpook National University Thesis: 2007.; Choi Seung-hyun, Chosun University Thesis: 2003.; Cho Kwang-yeon, Korea Research Institute of Chemical Technology, 2001).

Staphylococcus aureus, Inoculated in a liquid medium 2 days before the start of the experiment using the strain was incubated in a 37 ℃ incubator. After diluting to 1/100, 0.5μl was taken and applied to LB agar growth medium. Samples were added to an 8 mm paper dish at 40 μl each and placed on the prepared agar plate and incubated for 2 days to measure the growth inhibition region of the bacteria around the paper disk.

Strain sample Clear zone (mm)
Staphylococcus aureus C. water 0 1.the amount of 2 2. Second Chaff Vinegar 5 3. Third Chaff Vinegar 5 4. Leafy Chaff Vinegar 3 5. Wormwood Chaff Vinegar 6

As a result, the antimicrobial activity of the secondary and tertiary tablets of rice husk extract was high, and the antimicrobial activity of mugwort was higher. However, it was confirmed that anti-microbial activity was low in the case of A.

An example of the formulation of a cosmetic composition comprising the purified chaff extract of the present invention will be described, but the present invention is not intended to limit the present invention but is only intended to be described in detail.

Manufacturing example  1. flexible lotion ( skin Manufacturing

Table 1 shows an example of the preparation of the flexible skin lotion (skin) in the cosmetic composition containing purified chaff.

[Table 1]

Manufacturing example  2. Preparation of nutrient lotion (lotion)

Table 2 shows an example of preparing nutritious longevity (lotion) in cosmetics containing purified chaff.

TABLE 2

Manufacturing example  3. Preparation of nutrition cream

Table 3 shows an example of the preparation of nutrition cream in cosmetics containing purified chaff.

[Table 3]

Manufacturing example  4. Preparation of Essence

Table 4 shows an example of the preparation of the essence in the cosmetic composition containing purified chaff vinegar solution.

[Table 4]

Manufacturing example  5. Oil painting  Manufacture of foundation

Table 5 shows an example of the preparation of an emulsified foundation in cosmetics containing purified chaff.

TABLE 5

Manufacturing example  6. Preparation of Hydrophilic Ointment

TABLE 6

Claims (12)

Cosmetic composition containing chaff distilled distillate purified liquid showing anti-aging, antioxidant and antibacterial activity as an active ingredient. The method of claim 1,
The chaff herb distillation purification liquid is a "primary distillation purification liquid", "secondary distillation purification liquid" or "third distillation purification liquid" cosmetic composition. The method of claim 2,
The chaff distilled distillate is carbonized with chaff at 200 ° C. to 800 ° C. for 10 to 200 minutes using a carbonizer and condenses the smoke discharged during the carbonization to obtain crude chaff distillate, and A first step of distilling crude chaff herb distillate under reduced pressure and distilling it at a low temperature of 20 ° C. to 90 ° C. to obtain a primary distillate of chaff herb; A second step of adding 1% to 3% of activated carbon to the rice hull distillate, followed by heating and stirring to obtain a secondary distillation purified liquid after filtration of a filter press; After the addition of the oxide to the second distillation purification liquid obtained in the second step, the cosmetic composition is chaff herb distillation purification liquid obtained through a process comprising a third step of heating and stirring to obtain a third distillation purification liquid. A cosmetic composition containing as an active ingredient chaff herb distilled herbal extract showing anti-aging, antioxidant and antibacterial activity. The method of claim 4, wherein
The chaff herb distillate herbal liquor is one or more chaff herb distillate selected from the group consisting of "Chuju herb first distillate", "Curdough herb second distillate" A cosmetic composition, which is chaff herb distilled herbal herbal liquor obtained by distilling one or more herbal extracts selected from the group consisting of barley leaf, mugwort, gujippong and Chinese quince extract. The method according to claim 1 or 4,
The cosmetics include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam Cosmetic composition, characterized in that the formulation of any one selected from the group consisting of cleansing lotion, cleansing cream, body lotion and body cleanser. An external skin pharmaceutical composition containing chaff distilled distillate purified liquid showing anti-aging, antioxidant and antibacterial activity as an active ingredient. The method of claim 7, wherein
The chaff herb distillation purification liquid is a "primary distillation purification liquid", "secondary distillation purification liquid" or "tertiary distillation purification liquid". A pharmaceutical composition comprising, as an active ingredient, chaff herb distilled herbal extract showing anti-aging, antioxidant and antibacterial activity. The method of claim 9,
The chaff herb distillate herbal liquor is one or more rice hull distillation selected from the group consisting of "Churd herb first distillate", "Curdough herb second distillate" or "Churd herb third distillate" of claim 8 A pharmaceutical composition, which is a chaff herb distilled herbal herb obtained by distilling one or more herbal extracts selected from the group consisting of barley leaf, mugwort, gujippong and Chinese quince extract. The method according to claim 7 or 9,
The composition is an external skin pharmaceutical composition of cream, gel, patch, spray, ointment, warning, lotion, linen, or pasta. Carbonize the chaff at 200 ° C. to 800 ° C. for 10 to 200 minutes using a carbonizer and condense the smoke discharged during the carbonization process to obtain crude chaff herb distillate, and then depressurize the crude chaff herb distillate A first step of distilling at a low temperature of 20 ° C. to 90 ° C. to obtain a primary distillate of rice hulls; A second step of adding 1% to 3% of activated carbon to the rice hull distillate, followed by heating and stirring to obtain a secondary distillation purified liquid after filtration of a filter press; The method according to claim 1, further comprising adding a 1% to 5% (v / v) oxide to the secondary distillation purification liquid obtained in the second step, and then heating and stirring to obtain a tertiary distillation purification liquid. A manufacturing method for preparing the chaff distilled purified liquid.

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