KR20230150701A - A topical composition comprising prunus cerasus extract - Google Patents

Abstract

The present invention relates to a functional cosmetic composition containing tart cherry extract as an active ingredient. Specifically, the functional cosmetic composition containing tart cherry extract as an active ingredient includes a composition for improving or preventing acne using tart cherry extract as an active ingredient, a cosmetic composition for improving or preventing wrinkles using tart cherry extract as an active ingredient, or a tart cherry extract. It relates to a whitening composition containing active ingredients.
In particular, in relation to the acne improvement or prevention effect of the present invention, the tart cherry extract is an acne-causing bacteria , Propionibacterium acnes , Staphylococcus epidermis , and Staphylococcus aureus. aureus ), and has excellent anti-inflammatory and antioxidant activities. Since it is made from tart cherry, which is used for food and medicine, it has the advantage of not causing problems with resistance or safety.

Description

External composition containing tart cherry extract as an active ingredient {A TOPICAL COMPOSITION COMPRISING PRUNUS CERASUS EXTRACT}

The present invention relates to a composition for external use containing a plant extract, specifically tart cherry extract, as an active ingredient.

Human skin is the largest organ of the human body, accounting for 15% to 20% of body weight, and is the largest organ of the human body that protects various organs inside the skin from the external environment.

In addition, the skin performs various physiological roles, such as a barrier function that prevents moisture and oil-soluble components inside the body from leaking out of the skin, a function that maintains temperature through temperature control to regulate the body's health, an excretory function, and a respiratory function. I'm doing it.

Meanwhile, cosmetics, which in the past were thought to be products used only by women, are now used by people of all ages and genders as household necessities, not just tools for expressing beauty. As the industry develops, there is a demand for higher quality and diversification of cosmetics. As the number increases, interest in functional products that are expected to have special effects or efficacy is increasing.

Functional cosmetics currently recognized in Korea include whitening products that help whiten the skin, anti-wrinkle products that help improve wrinkles on the skin, and sunscreen products that help burn the skin beautifully or protect the skin from ultraviolet rays. , In addition, there are products for improving or relieving acne or atopic skin diseases.

In general, these functional cosmetics are interpreted as cosmetics that not only have cosmetic effects but also affect the structure or function of the body and have preventive, improving, and therapeutic effects. In particular, recently, as the proportion of functional cosmetics sales in total cosmetic sales has increased significantly, research is being actively conducted to find new effective ingredients for the human body and to use them efficiently on the skin.

Hormones or compound preparations have been mainly used as raw materials for these functional cosmetics, but these hormone preparations or compound preparations have problems with side effects, so in order to reduce these side effects, research has recently been conducted on natural preparations.

Preparations made from the above natural products have few side effects, are generally mild and long-lasting in their action, sometimes contain poorly soluble ingredients as auxiliary ingredients, and are known to be highly biodegradable and environmentally friendly. Examples of such natural product preparations include cosmetic compositions containing as active ingredients, such as yellow lotus root, red peony, turmeric, gardenia, Japanese knotweed extract, or salicylic acid, but cosmetics using tart cherry, specifically compositions for improving or preventing acne or preventing wrinkles. Alternatively, the improvement composition has not yet been disclosed.

KR 10-2198708B KR 10-0795225B KR 10-1507889B KR 10-2085422B

The present invention is intended to improve the problems of the prior art as described above. In particular, the present invention provides the active ingredients and optimal content of a cosmetic composition containing natural substances with few side effects while strengthening or imparting functionality to the skin. The purpose is to

In order to achieve the above object, the present invention provides a cosmetic composition for improving or preventing acne containing tart cherry ( Prunus cerasus ) extract as an active ingredient.

The tart cherry extract may preferably be extracted with water, an alcohol having 1 to 6 carbon atoms, or a mixture thereof as an extraction solvent, and more preferably, an aqueous solvent may be extracted with an extraction solvent.

Additionally, the tart cherry extract may have antibacterial activity against Propionibacterium acnes , Staphylococcus epidermis , and Staphylococcus aureus .

In addition, in order to achieve the above object, the present invention provides a cosmetic composition for improving or preventing wrinkles containing tart cherry ( Prunus cerasus ) extract as an active ingredient.

The tart cherry extract may be preferably extracted with water, an alcohol having 1 to 6 carbon atoms, or a mixture thereof as an extraction solvent, and more preferably with ethanol as an extraction solvent.

In order to achieve another object, the present invention provides a whitening cosmetic composition containing tart cherry ( Prunus cerasus ) extract as an active ingredient.

The tart cherry extract may be preferably extracted with water, an alcohol having 1 to 6 carbon atoms, or a mixture thereof as an extraction solvent, and more preferably with ethanol as an extraction solvent.

Unless otherwise specified herein, an active ingredient refers to an ingredient that provides an acne alleviating, improving or preventing effect in a cosmetic composition for improving or preventing acne, and in a cosmetic composition for improving or preventing wrinkles, it refers to an ingredient that prevents or prevents wrinkles. It refers to an ingredient that provides an improving effect, and in a whitening cosmetic composition, it refers to an ingredient that provides a whitening effect.

Unless otherwise specified herein, prevention refers to all actions that suppress the symptoms or delay the progression of a specific disease by administering the composition of the present invention.

Unless otherwise specified herein, treatment refers to any ameliorating action that improves or beneficially changes the symptoms of a specific disease by administering the composition of the present invention.

Unless otherwise specified herein, an individual refers to any animal, such as a human, monkey, dog, goat, pig, or rat, whose symptoms can be improved by administering the composition of the present invention to a specific disease, including gout. .

Unless otherwise specified herein, administration means providing a given composition of the present invention to an individual by any suitable method.

While the inventors of the present invention were researching materials having functional cosmetic raw materials derived from natural substances without problems with stability such as side effects or tolerance, they discovered that among various plants, tart cherry extract had an effect for improving or preventing acne, specifically treating acne. Not only does it have excellent antibacterial activity against the causative bacteria Propionibacterium acnes , but it also has excellent antibacterial activity against other skin flora, and unlike ordinary plant extracts, this antibacterial activity is better than alcohol or organic solvents such as ethanol. It was confirmed to be superior in water solvents, and it was also confirmed to have antioxidant and anti-inflammatory activities.

In addition, it was confirmed that the tart cherry extract has excellent elastase inhibitory activity and the ability to induce collagen synthesis in relation to the cause of wrinkles, and when ethanol is used as an extraction solvent compared to an aqueous solvent, the elastase inhibitory activity This was confirmed to be superior, and it was confirmed that the tart cherry extract inhibits the biosynthesis of melanin, a substance that causes skin pigmentation. When ethanol is used as an extraction solvent compared to an aqueous solvent, the effect of inhibiting melanin biosynthesis is greater. It was confirmed to be excellent.

The tart cherry extract, unlike the chemical ingredients used in existing acne improvement or prevention compositions, wrinkle improvement or prevention compositions, or whitening compositions, does not have safety or tolerance problems and does not cause formulation problems, so the tart cherry extract does not cause any problems with the formulation. It was confirmed that cherry extract can be used as an active ingredient in a composition for improving or preventing acne, a composition for improving or preventing wrinkles, or a composition for whitening, and based on this, the present invention was completed.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a composition for improving or preventing acne containing tart cherry ( Prunus cerasus ) extract as an active ingredient. The tart cherry extract may be a water extract using an aqueous solvent as an extraction solvent. The composition for improving or preventing acne can be used for pharmaceutical or cosmetic purposes.

In this aspect, the composition for improving or preventing acne may be a pharmaceutical composition for external use on the skin for improving or preventing acne or a cosmetic composition for improving or preventing acne.

The tart cherry extract is a water extract, for example, after drying tart cherries washed in running water, adding a water solvent, that is, water, to the dried tart cherries, pulverizing them using a grinder (homogenization), standing, and centrifuging. It may be prepared by performing a method of obtaining a supernatant.

The tart cherry (sour cherry, dwarf cherry, Prunus cerasus ) is a round or egg-shaped mature fruit of Shinyang cherry tree ( Prunus cerasus L.) belonging to the Prunus genus of the Rosaceae family, and is also called Shinyang cherry. It is called. The tart cherry is about 2.5 cm in diameter, ripens red in June to July, has a strong sour taste, and is a type of cherry that is currently widely used for food, and is also called sour cherry or pie cherry.

The tart cherry has recently been known to be good for health, and health functional foods and related products such as tart cherry juice are currently popular, but research on using it as a cosmetic material is insufficient.

In this specification, tart cherry extract is obtained by extracting tart cherry using a suitable solvent, and includes all forms of extract, diluted or concentrated extract, dried product obtained by drying the extract, or crude or purified products thereof. . Therefore, in a broad sense, the tart cherry extract of the present invention includes tart cherry processed products formulated so that tart cherry can be administered to animals, such as tart cherry powder.

The tart cherry extract can be obtained by extracting tart cherry with one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, and a subcritical or supercritical fluid, but is not limited thereto. The organic solvent having 1 to 6 carbon atoms includes alcohol, acetone, ether, benzene, chloroform, ethyl acetate, and methylene chloride. chloride), hexane, or cyclohexane.

Preferably, the extraction solvent may be water, an alcohol having 1 to 6 carbon atoms, or a mixture thereof extracted with an extraction solvent, and more preferably, it may be an aqueous solvent, that is, water.

The tart cherry extract can be prepared using conventional extraction methods in the industry, such as heat extraction, cold extraction, ultrasonic extraction, filtration, and reflux extraction. For example, tart cherry is mixed with a solvent using a homogenizer, crusher, or grinder. It can be obtained by pulverizing it, leaving it standing and centrifuging to obtain the supernatant, or extracting it under ultra-high pressure conditions of 100 MPa or more, preferably 100 MPa to 1000 MPa. In addition, if necessary, it can be prepared by additionally including filtration and concentration steps according to methods known in the art. The tart cherries can be purchased and used commercially, or those collected or cultivated in nature can be used.

In one embodiment of the present invention, the tart cherry extract is mixed with an extraction solvent at a weight ratio of 1:0.1 to 25, preferably 1:1 to 10, more preferably 1:1.5 to 3, and stored at room temperature. Or pulverize with a solvent using a homogenizer, grinder, or grinder at 5 to 70 ℃, or 10 to 30 ℃, leave for 5 to 50 hours, preferably 10 to 30 hours, and centrifuge to obtain the supernatant. The extract can be prepared by extracting and then concentrating under reduced pressure.

Meanwhile, in this specification, the term including as an active ingredient means including an amount sufficient to achieve the efficacy or activity of the tart cherry extract. As an example, the tart cherry extract can be used at a concentration of 0.5 to 1,000 mg/ml, preferably 10 to 500 mg/ml, and more preferably 20 to 100 mg/ml. Since the tart cherry extract is a natural product and has no side effects on the human body even when administered in excessive amounts, the upper quantitative limit of the tart cherry extract contained in the composition of the present invention can be selected and implemented by a person skilled in the art within an appropriate range.

In addition, the tart cherry extract may be a solvent extract extracted with an extraction solvent, a fraction obtained by adding a fractionating solvent to an extract prepared by extraction with an extraction solvent, or a purified product obtained by performing chromatography on the fraction.

The extraction solvent may be any one selected from the group consisting of water, organic solvents, or mixed solvents thereof that can be used for natural product extraction, preferably water, alcohols having 1 to 6 carbon atoms, and mixtures thereof. The organic solvent may be a straight-chain or branched alcohol with 1 to 6 carbon atoms such as methanol or ethanol, a polar solvent such as ethyl acetate or acetone, a nonpolar solvent such as nucleic acid or dichloromethane, a neutral solvent such as a ketone with 3 to 5 carbon atoms, or these. It may be a mixed solvent, preferably an alcohol having 1 to 6 carbon atoms, and more preferably ethanol.

The tart cherry extract of the present invention may be prepared according to a conventional plant extract manufacturing method. More specifically, the preparation of the tart cherry extract was performed by adding an extraction solvent, preferably an aqueous solvent or water, to the tart cherry and extracting it. The extraction method using the solvent may be chromatographic extraction, hot water extraction, cold needle extraction, warm needle extraction, pressure extraction, reflux extraction, or ultrasonic pulverization extraction, preferably chromatographic extraction.

In addition, the preparation of fractions of the tart cherry extract can be performed by adding a fractionating solvent to the extract prepared by the above extraction method, that is, crude extract or crude extract, and then obtaining fractions according to the polarity of the fractionating solvent.

The fractionation method by layer separation uses various chromatographies such as silica gel column chromatography, thin layer chromatography (TLC), and high performance liquid chromatography (HPLC). It can be done.

Additionally, after performing extraction, fractionation, or purification, the extract may be concentrated or the solvent may be removed by performing a reduced-pressure filtration process or additional concentration and/or freeze-drying. Therefore, the tart cherry extract in the present invention is used to include the dried product of the extract dried in a conventional method, the concentrate of the extract concentrated in the conventional method, and the diluted solution of the extract, dried product, or concentrate. The obtained tart cherry extract can be stored in a deep freezer until use.

Tart cherry extract, which has antibacterial activity against the acne-causing bacteria, antioxidant activity, and anti-inflammatory activity, can be used to alleviate, improve, or prevent acne, so it can be used to improve or prevent acne containing the tart cherry extract of the present invention as an active ingredient. The composition may be applied as a pharmaceutical composition for external application to the skin for improving or preventing acne or as a cosmetic composition for improving or preventing acne.

The tart cherry extract prepared by the production method of the present invention may preferably be a tart cherry water solvent extract or a tart cherry water extract. The tart cherry water extract is not only superior to the ethanol extract in terms of antibacterial effect against acne bacteria, but also uses water, which is the safest solvent, so there are no safety issues such as side effects or toxicity, and the extraction yield is excellent, so the acne Compositions for improvement or prevention have excellent marketability in many aspects.

In this respect, the present invention may be a pharmaceutical composition for external use on the skin for improving or preventing acne containing tart cherry extract as an active ingredient. The tart cherry extract may preferably be a water extract.

The pharmaceutical composition for external use on the skin for improving or preventing acne may further include appropriate carriers, excipients, and diluents commonly used in the preparation of pharmaceutical compositions.

The above skin external pharmaceutical compositions for improving or preventing acne can be formulated according to conventional methods, and are preferably formulated as creams, gels, patches, sprays, ointments, warning agents, lotions, liniment agents, pasta agents or catalyzers. It may be formulated as a plasma agent, but is not limited thereto.

Carriers, excipients, and diluents that may be included in the skin external pharmaceutical composition for improving or preventing acne include lactose, dextrose, sucrose, oligosaccharides, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, Gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When the pharmaceutical composition for external use on the skin for improving or preventing acne is formulated, commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, or surfactants may be used.

Preparations for parenteral administration of the skin external pharmaceutical composition for improving or preventing acne may include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories.

The non-aqueous solvent or suspension may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Additionally, witepsol, macrogol, tween 61, cacao, laurin, or glycerogeratin may be used as a base for the suppository.

The preferred dosage of the skin external pharmaceutical composition for improving or preventing acne varies depending on the patient's condition and weight, degree of disease, drug form, administration route and period, but can be appropriately selected by a person skilled in the art. As an example, the composition of the present invention can be administered at 0.000001 to 100 mg/kg (active ingredient/body weight) per day, preferably 0.001 to 10 mg/kg (active ingredient/body weight), based on the active ingredient. However, the above dosage does not limit the scope of the present invention in any way. The external pharmaceutical composition for improving or preventing acne may be administered once a day or in divided doses.

In addition, in another aspect, the present invention may be a cosmetic composition for improving or preventing acne containing tart cherry extract as an active ingredient. The tart cherry extract may preferably be a tart cherry water extract.

Tart cherry extract, which is an active ingredient in the cosmetic composition for improving or preventing acne, has excellent antibacterial activity against acne-causing bacteria, has excellent antioxidant activity and anti-inflammatory activity, and is recognized as safe, making cosmetics and facial cleansers effective in improving or preventing acne. It can be used in various ways, etc. Products to which the above active ingredients can be added include, for example, cosmetics such as various creams, lotions, skins, cleansing products, face washes, soaps, treatments, and serums.

The cosmetic composition for improving or preventing acne may further include one or more selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamins can maintain the effect of the present invention and can be included as long as they can be mixed in cosmetics. Specifically, vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide. , folic acid or vitamin C, or their salts (thiamine hydrochloride, sodium ascorbate, etc.) or their derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.). . The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic methods, or chemical synthesis.

The oil-soluble vitamins can maintain the effect of the present invention and can be included as long as they can be mixed in cosmetics. Specifically, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (D-alpha tocopherol, D-alpha tocopherol , D-alpha tocopherol), etc., and their derivatives (ascorbic acid palmitate, ascorbic acid stearate, ascorbic acid dipalmitate, alpha tocopherol acetic acid, alpha tocopherol nicotinic acid, vitamin E, D-pantothenyl alcohol, D- It may be pantothenyl alcohol, pantothenyl ethyl ether, etc.). The oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic methods, or chemical synthesis.

The polymer peptide can be anything that can maintain the effect of the present invention and can be mixed into cosmetics, and can be specifically collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, or keratin. The high molecular weight peptide can be obtained by a conventional method such as purification from a microbial culture medium, an enzyme method, or a chemical synthesis method, and then through a purification process, and is usually obtained from natural products such as dermis of pigs or cows, silk fiber of silkworms, etc. It can also be used by purifying from .

The high molecular weight polysaccharide can maintain the effect of the present invention and can be included as long as it can be mixed in cosmetics. Specifically, it can be hydroxyethylcellulose, xanthan gum, sodium hyaluronate, or chondroitin sulfate, and their salts (sodium salt, etc.), etc. The chondroitin sulfate or its salt can usually be purified and used from mammals or fish.

The sphingolipid can maintain the effect of the present invention and can be included as long as it can be mixed in cosmetics. Specifically, it can be ceramide, phytosphingosine, and sphingolipid. The sphingolipids can be purified by conventional methods from mammals, fish, shellfish, yeast, or plants, or obtained by chemical synthesis.

The seaweed extract can maintain the effect of the present invention and can be included as long as it can be mixed in cosmetics. Specifically, it may be brown algae extract, red algae extract, green algae extract, etc., and the seaweed extract includes color purified from the seaweed extract. It also includes ginan, alginic acid, sodium alginate, and potassium alginate. The seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition, the cosmetic composition of the present invention may further include other ingredients commonly mixed in cosmetics. More specifically, other ingredients mixed in the above-mentioned conventional cosmetics include fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, and pH adjusters. , alcohol, coloring, fragrance, blood circulation stimulant, cooling agent, antiperspirant, or purified water.

The fat component may specifically be ester-based fat, hydrocarbon-based fat, silicone-based fat, fluorine-based fat, animal fat, or vegetable fat.

Examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, and isostearic acid. Socetyl, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, sebacic acid. Diethyl, diisopropyl adipate, isoalkyl neopentanoate, tri(capryl, capric acid)glyceryl, tri-2-ethylhexanoate trimethylolpropane, trimethylolpropane triisostearate, tetra-2- Pentaelislitol ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, ricinooleic acid. Cetyl, isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate, cetostearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di(capryl, capric acid)propylene glycol, Propylene glycol dicaprylate, neopentyl glycol dicapric acid, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldode neopentanoate. Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, Polyglycerol isostearate ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate , trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, Cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 1,2-stealoyl hydroxystearic acid. It may be socetyl, stearyl 1,2-stealoylhydroxystearate, or isostearyl 1,2-stealoylhydroxystearate.

The hydrocarbon-based oil may be, for example, squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, or petroleum jelly.

Examples of the silicone-based oil include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxanemethylcetyloxysiloxane copolymer, dimethylsiloxanemethylstealoxysiloxane copolymer, It may be alkyl-modified silicone oil or amino-modified silicone oil.

The fluorine-based oil may be perfluoropolyether or the like.

The animal oil or vegetable oil includes avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, Cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil, candelier wax. , carnaba wax, liquid lanolin, or hydrogenated castor oil.

The surfactant may specifically be an anionic surfactant, a cationic surfactant, or an amphoteric surfactant.

The anionic surfactant is, for example, fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng. Salt, alkylamide phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt or perfluoroalkyl phosphate ester. It may be, etc.

The cationic surfactant is, for example, alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, and behenyl bromide. It may be trimethylammonium, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, or quaternary ammonium salt of a lanolin derivative.

The amphoteric surfactant is, for example, carboxy beta type, amide beta type, sulfo beta type, hydroxysulfo beta type, amide sulfo beta type, phosphobetain type, aminocarboxylic acid salt type, imidazoline derivative type, or amide type. It may be an amine-type amphoteric surfactant, etc.

The pigment may be an organic pigment, an inorganic pigment, or a complex pigment thereof.

The inorganic pigments include, for example, silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and oxide. It may be aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and complexes thereof.

The organic pigments include, for example, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinyl. It may be benzene styrene copolymer, silk powder, cellulose, CI pigment yellow, CI pigment orange, and complexes thereof.

The organic powder includes, for example, metal soap such as calcium stearate; Alkyl phosphate metal salts such as sodium zinc cetilate, zinc laurylate, and calcium laurylate; Acyl amino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amidesulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; It may be polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, tetrafluoroethylene, etc.

The cosmetic composition for improving or preventing acne can be manufactured in any formulation commonly manufactured in the industry, for example, emulsion, cream, lotion, pack, foundation, lotion, beauty essence, hair cosmetics, etc. .

Specifically, the cosmetic composition for improving or preventing acne includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, It may be formulated as any one formulation selected from the group consisting of nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser, but is not limited thereto.

When the formulation of the cosmetic composition for improving or preventing acne of the present invention is a paste, cream or gel, the carrier ingredients include animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, Talc or zinc oxide may be used.

When the formulation of the cosmetic composition for improving or preventing acne of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as the carrier ingredient, especially in the case of a spray. Additionally, it may contain a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition for improving or preventing acne of the present invention is a solution or emulsion, a solvent, solvating agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, and benzyl. Alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan can be used.

When the formulation of the cosmetic composition for improving or preventing acne of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. Suspending agents such as microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the cosmetic composition for improving or preventing acne of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, and methyl tau. Latex, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

Additionally, the present invention relates to a cosmetic composition for improving or preventing wrinkles containing tart cherry ( Prunus cerasus ) extract as an active ingredient. The tart cherry extract may be an ethanol extract using ethanol as an extraction solvent.

The tart cherry extract is an ethanol extract of tart cherry, for example, after drying tart cherry washed in running water, adding a water solvent, that is, water, to the dried tart cherry, grinding using a grinder (homogenization), standing, and centrifuging. It may be prepared by performing separation to remove the supernatant, adding ethanol to the obtained precipitate, leaving it to stand, and then performing centrifugation to obtain the supernatant.

The tart cherry (sour cherry, dwarf cherry, Prunus cerasus ) is a round or egg-shaped mature fruit of Shinyang cherry tree ( Prunus cerasus L.) belonging to the Prunus genus of the Rosaceae family, and is also called Shinyang cherry. It is called.

The tart cherry extract is obtained by extracting tart cherry using a suitable solvent, and includes all forms of extract, diluted or concentrated extract, dried product obtained by drying the extract, or crude or purified products thereof. Therefore, in a broad sense, the tart cherry extract of the present invention includes tart cherry processed products formulated so that tart cherry can be administered to animals, such as tart cherry powder.

The tart cherry extract can be obtained by extracting tart cherry with one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, and a subcritical or supercritical fluid, but is not limited thereto. The organic solvent having 1 to 6 carbon atoms includes alcohol, acetone, ether, benzene, chloroform, ethyl acetate, and methylene chloride. chloride), hexane, or cyclohexane.

The extraction solvent may preferably be water, an alcohol having 1 to 6 carbon atoms, or a mixture thereof, and more preferably ethanol.

The tart cherry extract can be prepared using conventional extraction methods in the industry, such as heat extraction, cold extraction, ultrasonic extraction, filtration, and reflux extraction. For example, tart cherry is mixed with a solvent using a homogenizer, crusher, or grinder. It can be obtained by pulverizing it, leaving it standing and centrifuging to obtain the supernatant, or extracting it under ultra-high pressure conditions of 100 MPa or more, preferably 100 MPa to 1000 MPa. In addition, if necessary, it can be prepared by additionally including filtration and concentration steps according to methods known in the art. The tart cherries can be purchased and used commercially, or those collected or cultivated in nature can be used.

In one embodiment of the present invention, the tart cherry extract is mixed with an extraction solvent at a weight ratio of 1:0.1 to 25, preferably 1:1 to 10, more preferably 1:1.5 to 3, and stored at room temperature. Or pulverize with a solvent using a homogenizer, grinder, or grinder at 5 to 70 ℃, or 10 to 30 ℃, leave for 5 to 50 hours, preferably 10 to 30 hours, and centrifuge to obtain the supernatant. The extract can be prepared by extracting and then concentrating under reduced pressure.

Meanwhile, in this specification, the term "included as an active ingredient" means included in an amount sufficient to achieve the efficacy or activity of the tart cherry extract. As an example, the tart cherry extract can be used at a concentration of 0.5 to 1,000 mg/ml, preferably 10 to 500 mg/ml, and more preferably 20 to 100 mg/ml. Since the tart cherry extract is a natural product and has no side effects on the human body even when administered in excessive amounts, the upper quantitative limit of the tart cherry extract contained in the composition of the present invention can be selected and implemented by a person skilled in the art within an appropriate range.

In addition, the tart cherry extract may be a solvent extract extracted with an extraction solvent, a fraction obtained by adding a fractionating solvent to an extract prepared by extraction with an extraction solvent, or a purified product obtained by performing chromatography on the fraction.

The extraction solvent may be any one selected from the group consisting of water, organic solvents, or mixed solvents thereof that can be used for natural product extraction, preferably water, alcohols having 1 to 6 carbon atoms, and mixtures thereof. The organic solvent may be a straight-chain or branched alcohol with 1 to 6 carbon atoms such as methanol or ethanol, a polar solvent such as ethyl acetate or acetone, a nonpolar solvent such as nucleic acid or dichloromethane, a neutral solvent such as a ketone with 3 to 5 carbon atoms, or these. It may be a mixed solvent, preferably an alcohol having 1 to 6 carbon atoms, and more preferably ethanol.

The tart cherry extract of the present invention may be prepared according to a conventional plant extract manufacturing method. More specifically, the preparation of the tart cherry extract was performed by adding an extraction solvent, preferably an aqueous solvent or water, to the tart cherry and extracting it. The extraction method using the solvent may be chromatographic extraction, hot water extraction, cold needle extraction, warm needle extraction, pressure extraction, reflux extraction, or ultrasonic pulverization extraction, preferably chromatographic extraction.

In addition, the preparation of fractions of the tart cherry extract can be performed by adding a fractionating solvent to the extract prepared by the above extraction method, that is, crude extract or crude extract, and then obtaining fractions according to the polarity of the fractionating solvent.

The fractionation method by layer separation uses various chromatographies such as silica gel column chromatography, thin layer chromatography (TLC), and high performance liquid chromatography (HPLC). It can be done.

Additionally, after performing extraction, fractionation, or purification, the extract may be concentrated or the solvent may be removed by performing a reduced-pressure filtration process or additional concentration and/or freeze-drying. Therefore, the tart cherry extract in the present invention is used to include the dried product of the extract dried in a conventional method, the concentrate of the extract concentrated in the conventional method, and the diluted solution of the extract, dried product, or concentrate. The obtained tart cherry extract can be stored in a deep freezer until use.

Since the tart cherry extract having the elastase inhibitory activity and the ability to synthesize collagen can be used to improve or prevent wrinkles, the composition for improving or preventing wrinkles containing the tart cherry extract of the present invention as an active ingredient is used to improve or prevent wrinkles. It can be applied as a preventive cosmetic composition.

The tart cherry extract prepared by the production method of the present invention may preferably be a tart cherry ethanol extract. The tart cherry ethanol extract is not only superior to water extract in terms of elastase inhibitory activity and collagen synthesis ability in terms of wrinkle improvement or prevention, but also uses ethanol, the safest solvent among organic solvents, so it has no side effects or toxicity. Since safety is not an issue, the composition for preventing wrinkles or wrinkles has excellent marketability in many aspects.

Tart cherry extract, which is an active ingredient in the cosmetic composition for improving or preventing wrinkles, has excellent wrinkle prevention or improvement activity and is recognized as safe, so it can be used in a variety of cosmetics and facial cleansers that have a wrinkle improvement or prevention effect. Products to which the above active ingredients can be added include, for example, cosmetics such as various creams, lotions, skins, cleansing products, face washes, soaps, treatments, and serums.

The cosmetic composition for improving or preventing wrinkles containing the tart cherry extract as an active ingredient may further include one or more selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingo lipids, and seaweed extract. .

In addition, the cosmetic composition for improving or preventing wrinkles containing the tart cherry extract as an active ingredient may further include other ingredients usually mixed in cosmetics. More specifically, other ingredients mixed in the above-mentioned conventional cosmetics include fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, and pH adjusters. , alcohol, coloring, fragrance, blood circulation stimulant, cooling agent, antiperspirant, or purified water.

Additionally, the present invention relates to a cosmetic composition for whitening containing tart cherry ( Prunus cerasus ) extract as an active ingredient. The tart cherry extract may be an ethanol extract using ethanol as an extraction solvent.

The tart cherry extract is an ethanol extract of tart cherry, for example, after drying tart cherry washed in running water, adding a water solvent, that is, water, to the dried tart cherry, grinding using a grinder (homogenization), standing, and centrifuging. It may be prepared by performing separation to remove the supernatant, adding ethanol to the obtained precipitate, leaving it to stand, and then performing centrifugation to obtain the supernatant.

The tart cherry (sour cherry, dwarf cherry, Prunus cerasus ) is a round or egg-shaped mature fruit of Shinyang cherry tree ( Prunus cerasus L.) belonging to the Prunus genus of the Rosaceae family, and is also called Shinyang cherry. It is called.

The tart cherry extract is obtained by extracting tart cherry using a suitable solvent, and includes all forms of extract, diluted or concentrated extract, dried product obtained by drying the extract, or crude or purified products thereof. Therefore, in a broad sense, the tart cherry extract of the present invention includes tart cherry processed products formulated so that tart cherry can be administered to animals, such as tart cherry powder.

The tart cherry extract can be obtained by extracting tart cherry with one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, and a subcritical or supercritical fluid, but is not limited thereto. The organic solvent having 1 to 6 carbon atoms includes alcohol, acetone, ether, benzene, chloroform, ethyl acetate, and methylene chloride. chloride), hexane, or cyclohexane.

The extraction solvent may preferably be water, an alcohol having 1 to 6 carbon atoms, or a mixture thereof, and more preferably ethanol.

The tart cherry extract can be prepared using conventional extraction methods in the industry, such as heat extraction, cold extraction, ultrasonic extraction, filtration, and reflux extraction. For example, tart cherry is mixed with a solvent using a homogenizer, crusher, or grinder. It can be obtained by pulverizing it, leaving it standing and centrifuging to obtain the supernatant, or extracting it under ultra-high pressure conditions of 100 MPa or more, preferably 100 MPa to 1000 MPa. In addition, if necessary, it can be prepared by additionally including filtration and concentration steps according to methods known in the art. The tart cherries can be purchased and used commercially, or those collected or cultivated in nature can be used.

In one embodiment of the present invention, the tart cherry extract is mixed with an extraction solvent at a weight ratio of 1:0.1 to 25, preferably 1:1 to 10, more preferably 1:1.5 to 3, and stored at room temperature. Or pulverize with a solvent using a homogenizer, grinder, or grinder at 5 to 70 ℃, or 10 to 30 ℃, leave for 5 to 50 hours, preferably 10 to 30 hours, and centrifuge to obtain the supernatant. The extract can be prepared by extracting and then concentrating under reduced pressure.

Meanwhile, in this specification, the term "included as an active ingredient" means included in an amount sufficient to achieve the efficacy or activity of the tart cherry extract. As an example, the tart cherry extract can be used at a concentration of 0.5 to 1,000 mg/ml, preferably 10 to 500 mg/ml, and more preferably 20 to 100 mg/ml. Since the tart cherry extract is a natural product and has no side effects on the human body even when administered in excessive amounts, the upper quantitative limit of the tart cherry extract contained in the composition of the present invention can be selected and implemented by a person skilled in the art within an appropriate range.

In addition, the tart cherry extract may be a solvent extract extracted with an extraction solvent, a fraction obtained by adding a fractionating solvent to an extract prepared by extraction with an extraction solvent, or a purified product obtained by performing chromatography on the fraction.

The extraction solvent may be any one selected from the group consisting of water, organic solvents, or mixed solvents thereof that can be used for natural product extraction, preferably water, alcohols having 1 to 6 carbon atoms, and mixtures thereof. The organic solvent may be a straight-chain or branched alcohol with 1 to 6 carbon atoms such as methanol or ethanol, a polar solvent such as ethyl acetate or acetone, a nonpolar solvent such as nucleic acid or dichloromethane, a neutral solvent such as a ketone with 3 to 5 carbon atoms, or these. It may be a mixed solvent, preferably an alcohol having 1 to 6 carbon atoms, and more preferably ethanol.

The tart cherry extract of the present invention may be prepared according to a conventional plant extract manufacturing method. More specifically, the preparation of the tart cherry extract was performed by adding an extraction solvent, preferably an aqueous solvent or water, to the tart cherry and extracting it. The extraction method using the solvent may be chromatographic extraction, hot water extraction, cold needle extraction, warm needle extraction, pressure extraction, reflux extraction, or ultrasonic pulverization extraction, preferably chromatographic extraction.

In addition, the preparation of fractions of the tart cherry extract can be performed by adding a fractionating solvent to the extract prepared by the above extraction method, that is, crude extract or crude extract, and then obtaining fractions according to the polarity of the fractionating solvent.

The fractionation method by layer separation uses various chromatographies such as silica gel column chromatography, thin layer chromatography (TLC), and high performance liquid chromatography (HPLC). It can be done.

Additionally, after performing extraction, fractionation, or purification, the extract may be concentrated or the solvent may be removed by performing a reduced-pressure filtration process or additional concentration and/or freeze-drying. Therefore, the tart cherry extract in the present invention is used to include the dried product of the extract dried in a conventional method, the concentrate of the extract concentrated in the conventional method, and the diluted solution of the extract, dried product, or concentrate. The obtained tart cherry extract can be stored in a deep freezer until use.

Since the tart cherry extract having the ability to inhibit melanin synthesis can be used for whitening, the whitening composition containing the tart cherry extract of the present invention as an active ingredient can be applied as a whitening cosmetic composition.

The tart cherry extract prepared by the production method of the present invention may preferably be a tart cherry ethanol extract. The tart cherry ethanol extract is not only superior to the water extract in terms of melanin synthesis inhibition ability in terms of whitening activity, but also uses ethanol, the safest solvent among organic solvents, so safety such as side effects or toxicity is not an issue. The whitening composition has excellent marketability in many aspects.

Tart cherry extract, which is an active ingredient in the cosmetic composition for whitening, has excellent melanin synthesis inhibition ability and is recognized as safe, so it can be used in a variety of cosmetics and facial cleansers with a whitening effect. Products to which the above active ingredients can be added include, for example, cosmetics such as various creams, lotions, skins, cleansing products, face washes, soaps, treatments, and serums.

The whitening cosmetic composition containing the tart cherry extract as an active ingredient may further include one or more selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingo lipids, and seaweed extract.

In addition, the whitening cosmetic composition containing the tart cherry extract as an active ingredient may further include other ingredients usually mixed in cosmetics. More specifically, other ingredients mixed in the above-mentioned conventional cosmetics include fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, and pH adjusters. , alcohol, coloring, fragrance, blood circulation stimulant, cooling agent, antiperspirant, or purified water.

As described above, the tart cherry extract, which is an active ingredient of the present invention, has excellent antibacterial activity against acne-causing bacteria in relation to the effect of improving or preventing acne, inhibits elastase activity and inhibits collagen in relation to the effect of preventing or improving wrinkles. It has excellent synthetic inducing properties and not only suppresses melanin production in relation to the whitening effect, but also uses tart cherry, which has been used for edible and medicinal purposes for a long time, and water, which is the safest solvent, or ethanol, which is recognized as the safest of organic solvents, so it has no side effects, etc. Because it does not cause problems, is recognized as safe, and has the advantage of being environmentally friendly, the composition of the present invention can be applied to alleviating, improving, or preventing skin acne, or preventing, improving, or whitening wrinkles.

Figures 1 to 3 confirm the acne alleviating, improving or preventing effect of tart cherry extract according to an embodiment of the present invention, depending on the extraction solvent and amount used. In Figures 1 to 3, TCE is the ethanol extract of tart cherry. , and TCW refers to the water extract of tart cherry, that is, water extract.
Figure 1 shows the antioxidant effect according to the amount of tart cherry extract used and the extraction solvent according to an embodiment of the present invention, where the horizontal axis represents the content of the extract, and the vertical axis is a graph showing the results of an experiment on radical removal ability. .
Figure 2 shows the antibacterial effect on acne-related harmful bacteria according to the extraction solvent of tart cherry extract according to an embodiment of the present invention, where A shows antibacterial activity against Propionibacterium acnes . B indicates antibacterial activity against Staphylococcus aureus , and C indicates antibacterial activity against Staphylococcus epidermis . In B and C, AMP refers to the ampicillin treatment group (AMP), which is a positive control group.
Figure 3 shows the anti-inflammatory effect according to the amount and extraction solvent of tart cherry extract according to an embodiment of the present invention, the horizontal axis shows the content of the extract, and the vertical axis is a graph showing the experimental results for NO scavenging ability. .
Figure 4 shows the whitening effect of tart cherry extract according to an embodiment of the present invention according to the extraction solvent and amount used. In Figure 4, TCE refers to the ethanol extract of tart cherry, and TCW refers to the water solvent of tart cherry. In other words, it means a water extract, the horizontal axis shows the content of the extract, and the vertical axis is a graph showing the results of an experiment on the degree of melanin production.
Figures 5 and 6 show the wrinkle improvement or prevention effect of tart cherry extract according to an embodiment of the present invention, depending on the extraction solvent and amount used. In Figures 5 and 6, TCE refers to the ethanol extract of tart cherry. And TCW refers to the water extract of tart cherry, that is, water extract.
Figure 5 shows the elastase inhibitory activity according to the amount of tart cherry extract used and the extraction solvent according to an embodiment of the present invention, where the horizontal axis represents the content of the extract and the vertical axis represents the elastase inhibitory activity. This is a graph showing the experimental results.
Figure 6 shows the anti-inflammatory activity according to the amount of tart cherry extract used and the extraction solvent according to an embodiment of the present invention. The horizontal axis represents the content of the extract, and the vertical axis represents the degree of collagen (Type 1 procollagen) production. This is a graph showing the experimental results.
Figure 7 shows the cytotoxicity of tart cherry extract according to an embodiment of the present invention, confirmed according to the extraction solvent and amount used. In Figure 7, TCE refers to the ethanol extract of tart cherry, and TCW refers to the water solvent of tart cherry. In other words, it means water extract, the horizontal axis shows the content of the extract, and the vertical axis is a graph showing the experimental results of cell viability.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

All experimental results were repeated at least three times and expressed as an average value. Comparison and statistical analysis with each group were performed using Student's test. Statistical significance was verified when each confidence interval p -value < 0.05 was considered significant. It was determined that there was.

[Example. [Confirmation of functional cosmetic effects of tart cherry extract]

Manufacturing example.

The tart cherry extract used in this experiment was prepared as follows.

The tart cherry ( Prunus cerasus ) used in this study was purchased as a dried product on the market, and 2.4 L (w/v%) of distilled water was added to 1,200 g of tart cherry, stirred and pulverized by homogenization, and left to stand for 18 hours. . Afterwards, centrifugation (5,000 g Х 30 min) was performed, and the supernatant was concentrated using a vacuum concentrator (Rotary Evaporator, EYELA N-1000, Japan) and then evaporated with Waterman NO 2 (Adventec, Roshi Kaisha Ltd., Japan). ) It was filtered through filter paper and freeze-dried, and the sample was then named Tart-cherry water extracts (TCW). After standing for 18 hours, 1.4 L (w/v) of ethanol (EtOH) was added to 700 g of the centrifuged sediment, left at low temperature at 4°C for 24 hours, and then centrifugation and concentration were repeated. , the concentrated sample was freeze-dried and used in each step of the experiment, named tart-cherry ethanol extracts (TCE).

In addition, the strains used to measure activity against skin flora were obtained from the KCTC (Korean Collection for Type cultures) microbial strain bank of the Korea Research Institute of Bioscience and Biotechnology. Gram positive bacterial strains included B. subtilis PM125, M. luteus , S. aureus , S. epidermis , and Prophonibacterium as acne bacteria. Antibacterial activity was investigated against acnes (KCTC 3314). Gram negative bacterial strains include E. coli D31, E. aerogense , K. pneumonia , P. aerginosa , S. typhium , and V. parrhemoyticus , and antibiotic resistant strains include Methicillin Resistance Staphyloccus aureus (MRSA) CCARM 3561, 3115, and 3089. The microbes were purchased from the antibiotic-resistant strain bank CCARM (Culture Collection of Antimicrobial Resistance Microbes), and anti-fungal activity was measured against Candida albicance .

Example 1. Measurement of antioxidant activity of tart cherry extract

The antioxidant activity of tart cherry extract was measured by measuring the radical scavenging ability using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Specifically, 150 μL of a 0.2 mM DPPH solution dissolved in EtOH and 50 μL of tart cherry extract of each concentration were placed in a 96 well plate and reacted at room temperature, specifically 15°C to 20°C for 15 minutes, followed by ELISA Multiscan Reader (Thermo scientific, Finland). ) was measured at 517 nm. Radical scavenging activity (RSA, %) was expressed by calculating the absorbance reduction rate of the sample-added group and the non-added group using the following calculation formula 1, and the activity of L-Ascorbic acid (Sigma-Aldrich, USA) was compared as a standard sample ( Bondet et al ., 1997), and the results are shown in Figure 1.

[Calculation Formula 1]

Radical Scavenging Activity(RAS, %) = [1 - (Sample)/Control]Absorbance Х 100

As shown in Figure 1, the antioxidant activity of the tart cherry extract for the DPPH method was compared with the activity of 96.3% at a concentration of 100 μg/mL using strong L-Ascorbic acid as a standard sample, and the concentration of the sample was 31.25 and 62.5. , at 125, 250, and 500 μg/mL, TCE showed high activity of 49.6%, 62.9%, 77.8%, 79.7%, and 85.6%, and TCW also showed high activity of 39%, 59%, 76.8%, 85.7%, and 88.4%. It showed activity in a concentration-dependent manner.

Example 2. Measurement of antibacterial activity of tart cherry extract against skin flora

The antibacterial activity of tart cherry extract against skin flora was tested using the DISC method and the MIC method. Specifically, looking at the DISC method, culture was performed at a concentration of mid-logarithmic phase (OD ₅₇₀ = 0.1, 5 Х 10 ⁷ CFU/mL) in sterilized Tryptic soy broth (TSB, Merck & Co. Inc., Germany) medium. Afterwards, 200 μL of bacterial culture was plated on sterilized TSA medium. Afterwards, 100 μL of the sample was absorbed into the paper disc (8 mm diameter, Advantec No. 2, Toyo Roshi Kaisha Ltd., Japan) at a concentration of 500 μg/disc, the solvent was sufficiently dried, and the sample was placed in an incubator at 37°C. After culturing in an incubator for 18 hours, activity was measured by the size of the clear zone created around the disc (Lehrer et al., 1991).

Minimal Inhibition Concentrations (MIC) measurement method demonstrated antibacterial activity using the broth dilution method. Specifically, skin flora and microorganisms were adjusted to mid-logarithmic phase (OD ₅₇₀ = 0.1, 1 Х 10 ⁵ CFU/mL) in Muller-Hinton broth (Merck & Co. Inc., Germany) and then cultured in a 96 well plate. 100 μL of the bacterial culture Muller-Hinton broth and 100 μL of the sample solution diluted by concentration were sequentially added and cultured in an incubator at 37°C for 24 hours, using an ELISA Multiscan Reader (Thermo scientific, Finland). Measured at 570 nm. RCM broth (Reinforced Clostridial Medium, Merk, Germany) was used as a culture medium for acne bacteria, and was subcultured at 4-week intervals before use. 2 weeks before the experiment, 10 mL of broth was inoculated with 100 μL of microbial culture at 4-day intervals. This increased activity. P. acnes , an anaerobic bacterium, was placed in an anaerobic jar containing Gas Pak (Anaerocult ^® , Merk Darmastadt, Germany) and cultured in an incubator at 37°C.

Anti-fungal activity was measured using the liquid growth assay method, and Candida albicance KCTC7965 was cultured at 30°C for 48 hours using Potato dextrose broth (PDB, Merck & Co. Inc., Germany). The concentration of the fungus was diluted to mid-logarithmic phase (OD ₆₃₀ = 0.1, 1 Х 10 ⁶ CFU/mL), mixed with 100 μL of the bacterial culture medium and 100 μL of the tart cherry extract sample in a 96 well plate, and then incubated at 30°C. After culturing for 48 hours, it was measured at 630 nm using an ELISA Multiscan Reader (Thermo scientific, Finland). Ampicillin (Sigma-Aldrich, USA) was used as a positive control for comparison.

The anti-acne bacteria, antibacterial and antifungal activities are shown in Figure 2 and Table 1 below.

As shown in Figure 2, the antibacterial activity of the tart cherry extract against skin flora was expressed in terms of the size of the clear zone (mm) using the DISC diffusion method, and the size of the clear zone against skin flora was determined by the activity. Tart cherry thermal water extract (TCW) showed higher activity than ethanol extract (TCE).

Specifically, the activity against the acne strain Propionibacterimun acne ( Cutibacterium acne ) showed a TCW of 18 mm, TCE showed no activity, and Ampicillin (Sigma-Aldrich, USA), a positive control, showed activity against other samples. Since it was difficult to accurately measure activity due to its influence, only TCW and TCE were measured.

The activity against Staphyloccocus aureus had a TCW of 16 mm, and TCE, which is also an EtOH extract, did not show a clear zone. Staphyloccocus aureus , a skin flora, causes food poisoning worldwide and has strong resistance, so it is widely distributed in nature, such as in the air and soil. It is a microorganism that also exists on the skin of healthy people and animals. In particular, Staphylococal enterotoxin is the cause of disease. This happens.

Ampicillin showed activity against Staphyloccus epidermis , another skin flora, with an activity of 24 mm and TCW of 4 mm. Staphylococcus epidermidis is reported to be a pathogen that maintains the appropriate pH of the skin to defend against external bacteria, particularly worsens primary skin diseases and acne symptoms, inhibits blood cell aggregation and phagocytosis, and destroys the immune system.

In relation to the antibacterial activity against skin flora, it was confirmed that the aqueous tart cherry extract using water rather than ethanol as the solvent had the best activity against P. acne , a major cause of acne.

The results of checking the Minimal Inhibitory Concentration (MIC) of the tart cherry extract and comparing it with Ampicillin, a positive control, are shown in Table 1 below.

As shown in Table 1, the activity against Gram positive bacteria was greater than that against Gram negative bacteria, and although the concentrations of TCE and TCW activities were the same, Stphyloccus sp. In this case, TCE showed activity at a concentration of 250 μg/mL, and TCW, a hot water extract, showed activity at 125 μg/mL. In addition, it showed activity in P. acne, an acne-causing strain, at a concentration of 62.5 μg/mL, yielding the same results compared to the DISC method.

Staphylococcus aureus occurs secondary to various skin diseases and easily causes infections in the skin or soft tissues. It is often infected in patients with atopic dermatitis and is reported to be the causative bacterium for bacterial skin diseases. In particular, 77% of bacterial skin diseases were found in Methicillin Resistance Staphylococcus aureus (MRSA) stains, an antibiotic-resistant strain, and this study aims to investigate and report its activity against MRSA. Looking at the activity, the activity was confirmed at 250 μg/mL for MRSA CCARM3089, and >500 μg/mL for both MRSA CCARM3115 and MRSA CCARM3561 strains. As a result, the tart cherry extract showed greater antibacterial activity against skin flora than antibiotic-resistant strains and showed the greatest tendency to inhibit bacterial growth against P. acne, so the tart cherry extract, especially the tart cherry water solvent, showed the greatest tendency to inhibit bacterial growth against P. acne. The extract was confirmed to have sufficient potential as a cosmetic raw material to improve acne symptoms.

Activity against Gram negative bacteria was shown at a concentration of 200 to 500 μg/mL. E. coli D31 and Pseudomonas areoginosa showed a tendency to inhibit both TCE and TCW at a concentration of 250 μg/mL, while Enterobacter aerogense , Klebsiella pneumoniae , Salmonella typhium , and Vibroo parahemolyticus showed activity at a concentration of 500 μg/mL. .

From the above results, it was confirmed that tart cherry extract exhibits better activity against Gram positive bacteria than Gram negative bacteria, and anti-fungal activity was measured against Candida albicsnse . As a result, it was confirmed that it did not have antifungal activity.

Example 3. Measurement of anti-inflammatory activity of tart cherry extract

Nitrate was measured to confirm the anti-inflammatory activity of the tart cherry extract in addition to its antibacterial activity against skin flora, and the activity to inhibit nitic oxide formation was measured using a nitrite assay.

Specifically, NO ₂ concentration, which is used as an indicator of Nitrite Oxide (NO) synthesis, was measured using the RAW264.7 Macrophage cell line that induced an immune response with LPS, and was measured 24 hours after inoculation with a cell concentration of 5 Х10 ⁵ cells/well. After culturing for a while, the samples were processed, and the culture medium cultured for 24 hours was used for measurement.

NO activity in the cell culture was tested using Griess reagent (1% sulfonic acid, 0.1% N-1-naphthyl ethylenediamine dihydrochloride, 2.5% phosphoric acid). 100 μL of the cell culture was mixed with an equal amount of Griess reagent and reacted for 10 minutes at room temperature. Then, it was measured at 546 nm using an ELISA Multiscan Reader (Thermo scientific, Finland). In all experiments, fresh culture medium was used as a blank. After inducing an inflammatory response with the LPS, the results of confirming the anti-inflammatory response by measuring NO are shown in Figure 3.

As shown in Figure 3, the Negative control, which did not induce an LPS response as one of the comparison groups in which the sample was not treated, was confirmed to have a Nitrate production amount of 13.3 μM, and the Positive control, which induced an inflammatory response with LPS, was 38.4 μM. As a result of treating sample concentrations of 62.5, 125, 250, and 500 μg/mL, TCE and TCW were found to be 23.4, 22.3, 22.1, and 19 μM for TCE, and 24.5, 23.7, 22.1, and 20.1 for TCW. It was expressed in μM, and the results showed that it inhibited nitrate production in a concentration-dependent manner.

Example 4. Confirmation of whitening activity of tart cherry extract

Changes in skin color due to melanin cause hyperpigmentation such as spots, freckles, age spots, and spots. α-MSH, a hormone that induces skin pigmentation, is secreted from keratinocytes, and adjacent melanocytes are present in the cell membrane. They recognize α-MSH through the melanocortin-1 receptor (MC1R), a receptor that binds to them.

This combination suggests that the most important intracellular factor in melanin synthesis is a transcription factor called Microphthalmia-associated transcription factor (MITF), which is responsible for increasing the expression of tyrosinase, which catalyzes the final melanin production process. Tyrosinase with increased expression begins melanin synthesis in earnest.

In this respect, the whitening activity of the tart cherry extract was confirmed by measuring the inhibitory effect on melanin biosynthesis within cells.

Specifically, to confirm the inhibitory effect on melanin biosynthesis, cells were distributed at 1 Х 10 ⁵ cells/well in a 6-well plate and cultured for 24 hours, then samples were treated at different concentrations, and after 1 hour, 200 nM of α-MSH was added. (Sigma-Aldrich, USA) was treated with each cell and cultured for 72 hours. The cultured cells were washed with PBS, treated with 1N NaOH solution containing 10% DMSO, reacted at 60°C for 1 hour, and then absorbance was measured at 490 nm using an ELISA Multiscan Reader (Thermo scientific, Finland). The results are shown in Figure 4.

As shown in Figure 4, as a result of examining the melanin biosynthesis rate of tart cherry extract targeting B16F10 mouse melanoma cells, the concentration of Arbutin, a standard sample, was found to be 48% at a concentration of 500 ㎍/mL, and TCE and TCW is 95.2%, 94.6%, 84%, and 74.1% for TCE, and 98.2%, 94%, 90%, and 84% for TCW, according to sample concentrations of 62.5, 125, 250, and 500, respectively. Both extracts were confirmed to inhibit melanin synthesis in a concentration-dependent manner, and TCE, an ethanol extract, was found to have a significantly superior ability to inhibit melanin biosynthesis compared to TCW, an aqueous solvent extract.

Example 5. Confirmation of wrinkle prevention ability of tart cherry extract

In general, skin epithelial cells are known to be major components related to skin elasticity and wrinkles, along with collagen, elastin, and hyaluronic acid, which are major components that account for about 70 to 80% of the dry weight. In addition, although the activity of MMP-1, one of the enzymes that synthesizes and decomposes Type 1 collagen in the skin, is balanced, the synthesis of Type 1 collagen decreases in skin tissue where skin aging occurs due to internal and external factors, and MMP-1 It is believed that the enzyme activity of 1 increases.

Specifically, the anti-wrinkle effect of tart cherry extract was confirmed by measuring the porcine elastase inhibitory activity and Type-1 procollagen synthesis effect.

Specifically, elastase inhibitory activity was determined by adding 20 μL of sample at each concentration to 20 μL of substrate solution containing 1.5 mM N-succinyl-(Ala)3-p-nitroanilide dissolved in 50 mM Tris-Cl buffer solution (pH 8.0). 20 μL of porcine pancreatic elastase (1,000 to 1,500 U/mL) was added thereto, reacted in an aqueous solution at 25°C for 10 minutes, and absorbance was measured at 410 nm using an ELISA Multiscan Reader (Thermo scientific, Finland). Urosolic acid (Sigma-Aldrich, USA) was used as a standard material for measurement.

The inhibitory activity of each sample was expressed as the concentration (μg/mL) required to decompose N-Succinyl-(Ala)3-p-nitroanilide into N-succinyl-(Ala)3 and p-nitroanilide. For color correction, Tris-HCl buffer solution was added instead of the buffer solution in which N-succinyl-(Ala)3-p-nitroanilide was dissolved, and a sample with the same concentration as the experimental group was added, and the results were confirmed through the following calculation equation 2. , the results are shown in Figure 5.

[Calculation Formula 2]

Elastase inhibition ratio (%) = [(Exp. - Blank) / Control] ⅹ 100

As shown in Figure 5, the concentration of Urosolic acid, which is a standard sample for porcine elastase inhibitory activity to confirm the wrinkle improvement effect of tart cherry extract, is 50 μg/mL, and the concentration of the sample is 62.5, 125, 250, and 500 μg/mL. As a result of measurement in mL, the porcine elastase activity was 17.8% for the standard sample, 6%, 12.9%, 13.8%, and 16.8% for TCE, and 9.2%, 10.4%, 10.9%, and 11.4% for TCW. It was confirmed that TCE, an ethanol extract, had significantly superior elastase inhibitory activity compared to TCW, an extract.

In addition, in the dermal layer of the skin, Type I collagen and Type III collagen are present in a ratio of 80 to 85% and 15 to 20%, respectively, and are produced through the following process. The chain of pro-alpha 1 and pro-alpha 2 expressed in dermal fibroblasts forms a triple helix structure and is made into procollagen, a precursor of collagen, which is secreted outside the cell and other pro-collagen. By combining with collagen, complete type I collagen was created.

In addition, it is known to regulate the amount of collagen by producing collagenase, a collagenase that is one of the matrix metalloproteinases (MMPs).

Collagen synthesis promoting efficacy to confirm the wrinkle prevention effect was confirmed by measuring Type 1 pro-collagen synthesis promoting activity. Specifically, CCD-986sk human dermal fibroblasts (fibroblasts) were inoculated into a 24 well plate at a concentration of 5 Х 10 ⁴ cells/well and cultured for 24 hours. After washing the cells three times with PBS and culturing them with serum-free culture medium, samples were processed after 24 hours, and the culture medium cultured for 72 hours was collected and used for measurement. The degree of collagen synthesis in the cell culture was measured by measuring the collagen content using a Type 1 pro-collagen C-peptide assay kit (Takara Bio Inc, Korea), and the results are shown in Figure 6.

As shown in FIG. 6, the negative control without sample treatment is 600 μM, and the concentration of the sample was tested at 250 μg/mL and 500 μg/mL. In the case of TCE, it was confirmed to be 720 nM and 725 nM. , in the case of TCW, it was confirmed to be 732.5 nM and 755 nM, and the tart cherry extract was confirmed to have collagen synthesis promoting activity.

Example 6. Confirmation of cytotoxic effect of tart cherry extract

To examine the safety of the tart cherry extract, cell survival was measured on CCD986-sk human dermal fibroblasts using the CCK-8 assay kit, and the results are shown in Figure 7.

As shown in Figure 7, Control without sample treatment is defined as a survival rate of 100%, and the concentrations of samples used in the experiment are 12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL. It was implemented. First, TCE showed survival rates of 99%, 98%, 98%, and 90% at each concentration, and TCW showed survival rates of 99%, 99%, 99%, and 95.9%, with both extracts having a slight toxicity of less than 10%. It was confirmed that all were safe.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

Claims (9)

A cosmetic composition for improving or preventing acne containing tart cherry ( Prunus cerasus ) extract as an active ingredient. According to paragraph 1,
The tart cherry extract is a cosmetic composition for improving or preventing acne, characterized in that the tart cherry extract is extracted with water, alcohol having 1 to 6 carbon atoms, or a mixture thereof with an extraction solvent. According to paragraph 2,
A cosmetic composition for improving or preventing acne, characterized in that the tart cherry extract is extracted from a water solvent with an extraction solvent. According to paragraph 1,
The tart cherry extract is a cosmetic for improving or preventing acne, characterized in that it has antibacterial activity against Propionibacterium acnes, Staphylococcus epidermis , and Staphylococcus aureus. Composition. According to paragraph 1,
The tart cherry extract is a cosmetic composition for improving or preventing acne, characterized in that it has anti-inflammatory activity and antioxidant activity. A cosmetic composition for improving or preventing wrinkles containing tart cherry ( Prunus cerasus ) extract as an active ingredient. According to clause 6,
A cosmetic composition for improving or preventing wrinkles, characterized in that the tart cherry extract is extracted with ethanol. A cosmetic composition for whitening containing tart cherry ( Prunus cerasus ) extract as an active ingredient. According to clause 8,
A cosmetic composition for whitening, characterized in that the tart cherry extract is extracted with ethanol.