WO2012074172A1

WO2012074172A1 - Composition comprising a purified distillate of rice husk showing anti-bacterial and anti-wrinkle activity

Info

Publication number: WO2012074172A1
Application number: PCT/KR2011/002024
Authority: WO
Inventors: Young-Kyo Suh; Kil-Hwan Han; Joon Seok Byun; Jegyu Hwang; Suk-Kyung Kim; Sang-Han Lee
Original assignee: Daewon Global System Integration Co., Ltd.
Priority date: 2010-12-02
Filing date: 2011-03-24
Publication date: 2012-06-07
Also published as: KR20120060650A; KR101273435B1

Abstract

The present invention is related to a composition comprising a purified distillate of rice husk showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient and that the distillate shows potent anti anti-oxidative, anti-bacterial and anti-wrinkle activity through various experiments, therefore, it can be used as the effective and safe composition for treating and preventing skin aging.

Description

COMPOSITION COMPRISING A PURIFIED DISTILLATE OF RICE HUSK SHOWING ANTI-BACTERIAL AND ANTI-WRINKLE ACTIVITY

The present invention is related to a topical and cosmetic composition comprising a purified distillate of rice husk showing anti-oxidative, anti-bacterial and anti-wrinkle activity.

Skin aging is a complex biological phenomenon involving multifactor process due to the accumulation of altered molecular and structural modifications with time (Makrantonaki, E., and Zouboulis, C. C. (2007). Molecular mechanisms of skin aging: state of the art. Ann N Y Acad Sci 1119, 40-50.). Modifications of signaling pathway and accumulated damaged molecules, as well as incomplete repair are considered a hallmark of aging (Bertram, C., and Hass, R. (2008). Cellular responses to reactive oxygen species-induced DNA damage and aging. Biol Chem 389, 211-220). The skin aging is the body’s first line of defense that was damaged by oxidant and environmental elements, as: many of the properties of exposure on the integument being from an early stage on, and continue throughout the lifetime. The appearance of aged skin characteristically shows thin, dry, blemish, with some loss of skin-related cells property, especially in skin, damages can be generated by internal causes as well as by environmental factors. Most evident skin aging process is the progressive loss of skin tissue (Robert, L., Labat-Robert, J., and Robert, A. M. (2009). Physiology of skin aging. Pathol Biol (Paris) 57, 336-341).

In skin structure, morphological cutaneous tissue consists of two main layers: epidermis and dermis. The epidermis is composed of several cell layers such as keratinocytes and melanocytes. The initial epidermal response to environmental damage is a hyperproliferative repair response, with thickening of the epidermis, as well as loss of epidermis polarity. Among lots of functions, the epidermis acts as a protective layer against environmental injury, ultraviolet (UV), toxins, and microorganism.

Accordingly, the skin serves as a viable candidate for oxidative damage, and for the skin aging, the fibroblasts are particularly vulnerable to oxidative damage.

Rice husk is a by-product formed from rice processing with a proportion ratio of 20% based on the weight of rice. Although it had been regarded as an good energy source once, it has been classified into waste materials because of the appearance of better energy sources and its limited properties to be used as compost or a feedstuff such as low digestive efficiency or corrosiveness due to the compactly coated husk structure comprising abundant silicon component. There has been reported that it contains 27-30% carbohydrate, 35-46% crude fiber, 13-21% crude ash mainly consisting of silicon component, 2-3% crude protein and 0.3-0.8% crude lipids.

Accordingly, there have been efforts to develop new appropriate uses of rice husk which had been discarded since it has been regarded as a waste material till now.

However, there have been no disclosures or suggestions on the novel cosmetic use of a purified distillate of rice husk in the treatment and prevention of skin aging by due to its anti-oxidative, anti-bacterial and anti-wrinkle activity in any of above cited literatures, the disclosures of which are incorporated herein by reference.

Accordingly, the present inventors have tried to find new use of the purified distillate of rice husk prepared by specific preparation methods and finally, confirmed that the distillate shows potent anti anti-oxidative, anti-bacterial and anti-wrinkle activity through various experiments, i.e., the several anti-aging tests such as collagenase inhibitory test, elastase inhibitory test, electron donating activity test, SOD-like activity test, xanthine oxidase inhibitory test as well as anti-bacterial test such as paper disc test etc, therefore, it can be used as the effective and safe composition for treating and preventing skin aging.

Accordingly, it is an object of the present invention to provide a composition comprising a purified distillate of rice husk showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient.

Specifically, the present invention provides a topical pharmaceutical composition comprising a purified distillate of rice husk and a pharmaceutically acceptable carrier thereof an active ingredient for the treatment and prevention of skin aging.

The present invention also provides comprising a cosmetic composition comprising a purified distillate of rice husk as an active ingredient for the treatment and prevention of skin aging.

The present invention also provided a use of the purified distillate of rice husk for the preparation of therapeutic agent for the treatment and prevention of skin aging in mammal or human.

The term “purified distillate of rice husk” disclosed herein includes “the 1st purified distillate of rice husk”, “the 2nd purified distillate of rice husk”, and “the 3rd purified distillate of rice husk”.

The term “composition” disclosed herein specifically denotes an externally applied composition, such as a topical pharmaceutical composition or cosmetic composition.

The term “skin aging” disclosed herein includes skin problem with aging, such as skin pigmentation, skin acne, skin melasma, skin wrinkle etc.

It is an object of the present invention to provide a method of treating or preventing skin aging in mammal or human in need thereof comprising administering topically to said mammal or human with an effective amount of the purified distillate of rice husk, together with a pharmaceutically acceptable carrier thereof.

In an alternative embodiment of the present invention, the present invention provides a composition comprising a purified distillate of rice husk and combined herb showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient.

The term “combined herb” disclosed herein comprises at least one herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne.

The herb of the present invention and the above-described purified distillate of rice husk are mixed with a mixed ratio of preferably, 1: 0.0001-1 (v/w), more preferably, 1: 0.001-0.5 (v/w), most preferably, 1: 0.01-0.1 (v/w) in the present invention and Perilla frutescens var. acuta, and Artemisia princeps var orientalis is mixed with a mixed ratio of preferably, 1: 0.01-50 (w/w), more preferably, 1: 0.1-10 (w/w), most preferably, 1: 1-5 (w/w).

The term “extract” disclosed herein comprises the extract which can be extracted with at least one solvent selected from water, C_1-C₄lower alkyl alcohol such as methanol, ethanol, butanol, etc, propyleneglycol, glycerin, ethyl acetate, butyleneglycol, ether, or chloroform, preferably, water, methanol, ethanol, more preferably, water.

Accordingly, the present invention also provides a topical pharmaceutical composition comprising a purified distillate of rice husk and combined herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne, and a pharmaceutically acceptable carrier thereof an active ingredient for the treatment and prevention of skin aging.

The present invention also provides comprising a cosmetic composition comprising a purified distillate of rice husk and combined herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne as an active ingredient for the treatment and prevention of skin aging.

The present invention also provided a use of a purified distillate of rice husk and combined herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne for the preparation of therapeutic agent for the treatment and prevention of skin aging in mammal or human.

It is an object of the present invention to provide a method of treating or preventing skin aging in mammal or human in need thereof comprising administering topically to said mammal or human with an effective amount of a purified distillate of rice husk and combined herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne, together with a pharmaceutically acceptable carrier thereof.

Hereinafter, the present invention is described in detail.

For example, the inventive the 1st purified distillate of rice husk of the present invention may be prepared by the process comprising the steps: subjecting dried rice husk to carbonization with carbonizing machine, preferably, DCH-600 of Daewon GSI Corp. (Korea, www. Daewonces.co.kr) at the temperature ranging from 200 ℃ to 800 ℃, preferably, from 300 ℃ to 600 ℃, for the period ranging from 10 mins to 200 mins, preferably, 40 mins to 120 mins and condensing the discharged smoke from carbonization treatment to afford crude vinegar distillate of rice husk at 1st step; subjecting to distillation under reduced pressure at low temperature, preferably, 20 ℃ to 90 ℃, preferably, from 40 ℃ to 70 ℃, at the 2nd step to afford the 1st distillate of rice husk of the present invention.

The inventive the 2nd purified distillate of rice husk of the present invention may be prepared by the process comprising the steps: adding active carbon, preferably, in the amount of 1 to 3% (v/v) to the 1st purified distillate of rice husk, heating with stirring and filtering with filter press to obtain the 2nd purified distillate of rice husk of the present invention.

The inventive the 3rd purified distillate of rice husk of the present invention may be prepared by the process comprising the steps: adding oxides, preferably, hydrogen oxide in the amount of 1 to 5% (w/w) to the 2nd purified distillate of rice husk, heating with stirring and filtering with filter press to obtain the 3rd purified distillate of rice husk of the present invention.

Additionally, if necessary, at least one herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne, mixed with the appropriate mixed ratio may be further added to the purified distillate of rice husk with the appropriate mixed ratio.

The extract of herb of the present invention may be prepared as follows:

For example, the dried herbs of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, or Chaenomeles sinensis Koehne is sliced into small pieces. The purified distillate of rice husk was mixed therewith with appropriate ratio, the pieces is mixed with about 1 to 30-fold, preferably, 3 to 10-fold volume of distilled water, alcohols such as methanol, ethanol and the like, or the mixtures thereof, preferably, water; and is enfleuraged at the temperature ranging from 10℃ 120℃, preferably 15 to 90℃, for the period ranging from about 0.5 hour to 10 days, preferably 12 hours to 2 days with 2 to 5 times, by sonication, reflux or conventional extraction, preferably, reflux extraction to obtain herbal extract. The herbal extract is filtered and concentrated at 40 to 80℃ under reduced pressure. The extract is concentrated and then dried by freeze drying or vacuum drying to obtain purposed extract

It is confirmed that the inventive distillate and the combined extract of mixed herbs prepared by above-described method shows potent anti anti-oxidative, anti-bacterial and anti-wrinkle activity through various experiments, i.e., the several anti-aging tests such as collagenase inhibitory test, elastase inhibitory test, electron donating activity test, SOD-like activity test, xanthine oxidase inhibitory test as well as anti-bacterial test such as paper disc test etc, therefore, it can be used as the effective and safe composition for treating and preventing skin aging.

The inventive composition for treating and preventing purposed disease may comprise the above-described extract as 0.1 ~ 50 % by weight based on the total weight of the composition.

The inventive composition may additionally comprise conventional carrier, adjuvants or diluents in accordance with a using method well known in the art. It is preferable that said carrier is used as appropriate substance according to the usage and application method, but it is not limited. Appropriate diluents are listed in the written text of Remington’s Pharmaceutical Science (Mack Publishing co, Easton PA).

Hereinafter, the following formulation methods and excipients are merely exemplary and in no way limit the invention.

The composition according to the present invention can be provided as a pharmaceutical composition containing pharmaceutically acceptable carriers, adjuvants or diluents, e.g., lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starches, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinyl pyrrolidone, water, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate and mineral oil. The formulations may additionally include fillers, anti-agglutinating agents, lubricating agents, wetting agents, flavoring agents, emulsifiers, preservatives and the like. The compositions of the invention may be formulated so as to provide quick, sustained or delayed release of the active ingredient after their administration to a patient by employing any of the procedures well known in the art.

For example, for topical administration, the extract of the present invention can be formulated in the form of ointments and creams.

Pharmaceutical formulations containing present composition may be prepared in any form, including topical preparation such as cream, gel, liniment, ointment, lotion, gel, balm, patch, paste, spray solution, cataplasma, aerosol and the like.

The composition of the present invention in pharmaceutical dosage forms may be used in the form of their pharmaceutically acceptable salts, and also may be used alone or in appropriate association, as well as in combination with other pharmaceutically active compounds.

The desirable dose of the inventive extract or composition varies depending on the condition and the weight of the subject, severity, drug form, route and period of administration, and may be chosen by those skilled in the art. However, in order to obtain desirable effects, it is generally recommended to administer at the amount ranging 0.0001 to 1000mg/kg, preferably, 0.001 to 100 mg/kg by weight/day of the inventive extract of the present invention. The dose may be administered in single or divided into several times per day. In terms of composition, the amount of inventive extract should be present between 0.01 to 50% by weight, preferably 0.5 to 40% by weight based on the total weight of the composition.

The pharmaceutical composition of present invention can be administered to a subject animal such as mammals (rat, mouse, domestic animals or human) via various routes. All modes of administration are contemplated, for example, administration can be made orally, rectally or by intravenous, intramuscular, subcutaneous, intra-cutaneous, intrathecal, epidural or intra-cerebroventricular injection.

It is preferable that the present cosmetic composition contains 0.0001-90%, more preferably, 0.001- 70% , most preferably, 0.01- 50% by the weight of the inventive composition based on the total weight of the composition. The other components may be a mixture of the ingredients of a conventional cosmetic composition well known in the art.

Cosmetic formulations containing above composition may be prepared in any form such as skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, nutrient cream, moisture cream, hand cream, massage cream, essence, pack, foundation, cleansing water, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, soap, treatment, beauty solution and the like.

The cosmetic composition of the present invention can comprises additional additives selected from the group consisting of water soluble vitamin, lipid soluble vitamin, peptide polymer, polysaccharide polymer, sphingolipid and sea-weed extract.

Preferable water soluble vitamins are any one which can be mixed with cosmetic, however, various vitamin such as vitamin B_1, B₂, B₆, pyridoxine, pyridoxine HCl, vitamin B₁₂, pantothenic acid, nicotinic acid, nicotinamide, folic acid, vitamin C, vitamin H etc, the salt thereof such as thiamin HCl salt, ascorbic acid Na salt etc or their derivatives such as ascorbic acid-2-phosphonic acid Na salt, ascorbic acid-2-phosphonic acid Mg salt are preferable and those can be obtained by conventional method such as microbial conversion method, purification method from the microbial cultivates, enzymatic method or chemical synthetic method.

Preferable lipid soluble vitamins are any one which can be mixed with cosmetic, however, various vitamin such as vitamin A, D₂, D₃, E (dl-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and their derivatives such as palmitic acid ascorbate, stearic acid ascorbate, dipalmitic acid ascorbate, acetic acid- dl-α-tocopherol, nicotinic acid dl-αtocopherol vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethylether etc. including the lipid soluble vitamin used in examples of present invention are preferable and those can be obtained by conventional method such as microbial conversion method, purification method from the microbial cultivates, enzymatic method or chemical synthetic method.

Preferable peptide polymers are any one which can be mixed with cosmetic, however, collagen, hydrolysable collagen, gelatin, elastin, hydrolysable gelatin, keratin etc. including the peptide polymer used in examples of present invention are preferable.

Preferable polysaccharide polymers are any one which can be mixed with cosmetic, however, hydroxy ethyl cellulose, xanthin gum, hyaluronic acid Na, chondroitin sulfate or their salt (Na salt etc) and the like are preferable. For example, chondroitin sulfate or the salt thereof etc can be used by being purified from mammal or fishes ordinarily.

Preferable sphingolipid are any one which can be mixed with cosmetic, however, ceramide, pit-sphingosin, sphingo-lipopolysaccharide and the like are preferable. Sphingo-lipid can be obtained by being purified from mammal, fish, shellfish, yeast or plant etc in conventional method.

Preferable seaweed extract is any one which can be mixed with cosmetic, however, the extract of brown algae, red algae, green algae and the like or the purified carrageenan, alginic acid, arginic acid Na, K isolated therefrom are preferable. Algae extract can be obtained by being purified from seaweed in conventional method.

The cosmetic composition of the present invention may combine with other ingredients used in conventional cosmetic composition, if necessary, together with above described essential ingredient.

Preferable above described other ingredients may comprise oil ingredient, humectants, emollients, surfactants, organic or inorganic dye, organic powder, ultraviolet ray absorbing agent, preservatives, antiseptics, antioxidants, plant extract, pH controller, alcohol, pigments, perfumes, refrigerants, blood circulator, antihidrotic, distilled water etc.

Preferable oil ingredients may comprise ester oil, hydrocarbon oil, silicone oil, fluoride oil, animal oil, plant oil and so on.

Preferable ester oil described above may comprise glyceryl tri-2-ethyl hexanoic acid, cetyl 2-ethyl hexanoic acid, isopropyl myristic acid, butyl myristic acid, isopropyl palmitic acid, ethyl stearic acid, octyl palmitic acid, isocetyl isostearic acid, butyl stearic acid, ethyl linoleic acid, isopropyl linoleic acid, ethyl oleic acid, isocetyl myristic acid, isostearyl myristic acid, isostearyl palmitic acid, octyldodecyl myristic acid, isocetyl isostearic acid, diethyl sebasic acid, isopropyl adipic acid, isoalkyl neopetanoic acid, glyceryl tri(capryl, capric acid), trimethylopropane tri-2-ethyl hexanoic acid, trimethylopropane triisostearic acid, pentaerythritol tetra-2 ethyl hexanoic acid, cetyl caprylic acid, decyl lauric acid, hexyl lauric acid, decyl myristic acid, myristyl myristic acid, cetyl myristic acid, stearyl stearic acid, decyl oleic acid, cetyl licinoleic acid, isostearyl lauric acid, isotridecyl myristic acid, isocetyl palmitic acid, octyl stearic acid, isocetyl stearic acid, isodecyl oleic acid, octyldodecyl oleic acid, octyldodecyl linoleic acid, isopropyl isostearic acid, cetostearyl 2-ethyl hexanoic acid, stearyl 2-ethyl hexanoic acid, hexyl isostearic acid, ethylene glycol dioctanoic acid, ethylene glycol dioleic acid, propylene glycol dicapric acid, propylene glycol di(capryl, capric acid), propylene glycol dicaprylic acid, neopentylglycol dicapric acid, neopentylglycol dioctanoic acid, glyceryl tricaprylic acid, glyceryl triundecylic acid, glyceryl triisopalmitic acid, glyceryl triisostearic acid, octyldodecyl neopentanoic acid, isostearyl octanoic acid, octyl isononanoic acid, hexyldecyl neodecanoic acid, octyldodecyl neodecanoic acid, isocetyl isostearic acid, isostearyl isostearic acid, octyldecyl isostearic acid, polyglycerin oleanoic acid ester, polyglycerin isostearic acid ester, triisocetyl citric acid, triisoalkyl citric acid, triisooctyl citric acid, lauryl lactic acid, myristyl lactic acid, cetyl lactic acid, octyldecyl lactic acid, triethyl citric acid, acetyltriethyl citric acid, acetyl tributyl citric acid, trioctyl citric acid, diisostearyl maleic acid, di 2-ethylhexyl hydroxy stearic acid, 2-ethyl hexyl succinic acid, diisobutyl adipic acid, diisopropyl sebasinic acid, dioctyl sebacinic acid, cholesteryl stearic acid, cholesteryl isostearic acid, cholesteryl hydroxy stearic acid, cholesteryl hydroxy stearic acid, cholesteryl oleic acid, dihydrocholesteryl oleic acid, pitsteryl isostearic acid, pitsteryl oleic acid, isocetyl 12-stealoyl hydroxy stearic acid, stearyl 12-stealoyl hydroxy stearic acid, isostearyl 12-stealoyl hydroxy stearic acid.

Preferable hydrocarbon oil described above may comprise squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, vaselin and the like.

Preferable silicone oil may comprise polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethyl siloxane-methyl cetyloxysiloxan copolymer, dimethyl siloxane-methyl stealoxysiloxane copolymer, alkyl modified silicone oil, amino modified silicone oil and the like.

Preferable fluoride oil can comprise perfluoropolyether and the like.

Preferable animal or plant oil can comprise avocado oil, almond oil, olive oil, sesame oil, rice husk oil, safflower oil, soy-bean oil, corn oil, rape oil, amygdalin oil, palm kernel oil, palm oil, pimaja oil, sunflower oil, fruite seed oil, cotton seed oil, coconut palm oil cucui nut oil, wheat embryo bud oil, rice embryo bud oil, sia butter, evening-primrose oil, marker daymia nut oil, medo home oil, egg yolk oil, lanolin, hempseed oil, mink oil, orange ruppy oil, hohoba oil, carnawa wax, liquid lanolin, solid pimaja wax and the like.

Preferable humectants can comprise water-soluble low molecular humectants, lipophilic low molecular humectants, water-soluble polymer and lipid soluble polymer.

Specifically, preferable water soluble low molecular humectants can comprise cerin, glutamine, sorbitol, mannitol, pyrrolidone-carboxylic acid Na, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization index. >2), polypropylene glycol (polymerization index >2), lactic acid, lactate salt and the like.

Preferable lipid soluble low molecular humectants can comprise cholesterol, cholesteryl ester and the like.

Preferable water soluble polymer can comprise carboxy vinyl polymer, poly asparaginic acid salt, tragacanth, xanthin gum, HMC (hydroxy methyl celluose), HEC (hydroxy ethyl celluose), HPC (hydroxy propyl celluose), carboxymethylcellulose, water soluble chitin, chitosan, dextrin and the like.

Preferable lipid soluble polymer can comprise polyvinylpyrrolidone-eicocene copolymer, polyvinylpyrrolidone-hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, silicone polymer and the like.

Preferable emollients can comprise long chain acyl glutamic acid cholesteryl ester, cholesteryl hydroxy stearic acid, 12-hydroxy stearic acid, rogic acid, lanolin fatty acid cholesteryl ester and the like.

Preferable surfactant can comprise nonionic surfactants, anionic surfactants, cationic surfactants, ambivalent surfactants and the like.

Specifically, preferable non-ionic surfactants can comprise self-emulsified monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene (POE) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE solid pimaja oil, POE pimaja oil, POE-POP copolymer, POE-POP alkyl ether, polyether modified silicone, lauric acid alkanol amide, alkyl amine oxide, hydrogen addition soybean phospholipid and the like.

Preferable anionic surfactants can comprise fatty acid soap, ?-acyl sulfonic acid salt, alkyl sulfonic acid salt, alkyl ally sulfonic acid, alkyl naphthalene sulfonic acid salt, alkyl sulfonic acid salt, POE alkylether sulfate salt, alkyl amide sulfate salt, alkyl phosphate salt, POE alkyl phosphate salt, alkylamide phosphate salt, alkyloylalkyl taurine salt, N-acyl-amino acid salt, POE alkyl ether carboxylic acid salt, alkyl sulfo succinic aid salt, alkyl sulfo-acetic acid salt, acylated hydrolysable collagen peptide salt, perfluoro alkyl phosphate ester and the like.

Preferable cationic surfactant can comprise alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, setostearyltrimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, vehenyltrimethyl ammonium bromide, benzalkonium chloride, diethylamino ethyl amide stearic acid, dimethylaminopropyl amide stearic acid, lanolin derivatives quaternary ammonium and the like.

Preferable ambivalent surfactants can comprise carboxy betaine type, amide betaine type, hydroxy sulfo betaine type, phosphobetaine type, aminocarboxylic acid, imidazoline derivatives type, amide amine type and the like.

Preferable organic and inorganic dyes can comprise silicic acid, anhydrous silicic acid, magnesium silicic acid, talc, ceracyte, mica, caolin, bengala, clay, bentonite, titan film mica, oxy chlorine bismuth, zirconium oxide, magnesium oxide, zinc oxide, titan oxide, aluminium oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, ferrous oxide, chromium oxide, chromium hydroxide, calamine, carbon black and the complex thereof as an inorganic dyes; polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluoride resin, silicone resin, acryl resin, melamine resin, epoxy resin, polycarbonated resin, divinyl benzene-styrene copolymer, silk powder, cellulose, CI pigment yellow, CI pigment orange as an organic dyes; and their complex etc.

Preferable organic powder can comprise metal soap such as calcium stearate; alkyl phosphonate metal salt such as sodium zinc cetylic acid, zinc laurylic acid, calcium laurylic acid; acylamino acid polyvalent metal salt such as calcium N-lauroyl-β-alanine, zinc N-lauroyl-β-alanine, calcium N-lauroyl-glycine etc.; amide sulfonic acid polyvalent metal salt such as calcium N-lauroyl-taurine, calcium N-palmitoyl-taurine; N-acyl basic amino acid such as Nε-lauroyl-L-lysine, Nε-palmitoyl-lysine, Nα-palmitoyl ornitine, Nα-lauroly arginine, hardened lanolin fatty acid acyl arginine and the like; N-acylpolypeptide such as N-lauroylglycyl glycine; α-amino fatty acid such as α-amino caprylic acid, α-amino lauric acid and the like; polyethylene, polypropylene, nylon, polymethylmetacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and so on.

Preferable ultraviolet absorbing agents can comprise paraaminobenzoic acid, paraamonoethyl benzoate, paraamino amyl benzoate, paraamino octyl benzoate, ethyleneglycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, benzyl cinnamic acid, paramethoxy 2-ethoxy ethyl cinnamic acid, paramethoxy octyl cinnamic acid, diparamethoxy mono-2-ethylhexane glyceryl cinnamic acid, paramethoxy isopropyl cinnamic acid, diisopropyl-diisopropyl cinnamate ester mixture, urokanic acid, ethyl urokanic acid, hydroxy methoxy benzophenone, hydroxymethoxy benzophenone sulfonic acid and their salt, dihydroxy methoxy benzophenone, dihydroxy methoxy benzophenone disulfonate Na, dihydroxy benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4’-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2’-ethylhexyl-1’-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl) benzotriazole and the like.

Preferable preservatives can comprise hinokitiol, trichloric acid, trichlorohydroxydiphenylether, chlorohexidine glucuronate, phenoxyethanol, resorcine, isopropylmethylphenol, azulene, salicylic acid, zinc pilithione, bezalconium HCl, photosensitizer 301, mononitroguaiacol Na, undecylenic acid etc.

Preferable antioxidants can comprise butylhydroxyanisole, propyl gallate, ellisorbate and the like.

Preferable pH controller can comprise citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumaric acid, succinic acid, sodium succinic acid, sodium hydroxide, sodium hydrogen phosphate and the like.

Preferable alcohol can comprise cetyl alcohol etc.

Furthermore, other ingredient addable to above described component and the amount thereof is not limited within the scope of the purpose and effect of the present invention, however, it is preferable that the amount of the other ingredients ranges from 0.01 to 5%, more preferably, 0.01 to 3% in that of total composition.

The cosmetic composition of the present invention can be modified as a solution, emulsion, cohesive mixture etc.

Above described ingredients such as water-soluble vitamin, lipid soluble vitamin, peptide polymer, polysaccharide polymer, sphingolipid, sea weed extract and addable ingredients which can be added other than above described ingredients if necessary, can be obtained by conventional methods disclosed in the literature (Matsumoto Mithio; Manual for the development of transdermal applied preparation. Seisi Press, 1st Ed., 1985).

Inventive compounds of the present invention have no toxicity and adverse effect therefore, they can be used with safe.

It will be apparent to those skilled in the art that various modifications and variations can be made in the compositions, use and preparations of the present invention without departing from the spirit or scope of the invention.

The present invention is more specifically explained by the following examples. However, it should be understood that the present invention is not limited to these examples in any manner.

present invention provides a composition comprising a purified distillate of rice husk showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient and that the distillate shows potent anti anti-oxidative, anti-bacterial and anti-wrinkle activity through various experiments, therefore, it can be used as the effective and safe composition for treating and preventing skin aging.

The above and other objects, features and other advantages of the present invention will more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which;

Fig. 1 shows the flow chart of the preparation method of inventive distillates;

Fig. 2 shows the scavenging activity of inventive distillate on DPPH radical (Data is expressed as Mean±SD and result represents more than triplicate test);

Fig. 3 shows the anti-oxidant activity of inventive distillate on FRAP;

Fig. 4 shows the anti-oxidant activity of inventive distillate on;

Fig. 5 represents the inhibitory activity of inventive distillate on SOD-like activity (Data is expressed as Mean±SD and result represents more than triplicate test, p<0.05);

Fig. 6 represents the inhibitory activity of inventive distillate on xanthine oxidase activity (Data is expressed as Mean±SD and result represents more than triplicate test, p<0.05);

Fig. 7 represents the inhibitory activity of inventive distillate on collagenase enzyme activity (Data is expressed as Mean±SD and result represents more than triplicate test, p<0.05);

Fig. 8 represents the inhibitory activity of inventive distillate on elastase enzyme activity (Data is expressed as Mean±SD and result represents more than triplicate test, p<0.05).

EXAMPLES

The following Reference Example, Examples and Experimental Examples are intended to further illustrate the present invention without limiting its scope.

Example 1. The preparation of rice husk distillate

1-1. 1st purified distillate

Dried rice husk (Korea) was carbonized with carbonizing machine (DCH-600, Daewon GSI Corp. www. Daewonces.co.kr) at 500 ℃ for 60 mins and the discharged smoke was condensed to afford 100 liter of crude vinegar distillate of rice husk at 1st step (designated as “RH” hereinafter).. The crude vinegar distillate was distillated under reduced pressure at 50 ℃ to afford 900 liter of the 1st distillate of rice husk (designated as “RH-1” hereinafter).

1-2. 2nd purified distillate

2% (v/v) active carbon was added to the 1st purified distillate obtained from step 1-1 and heated with stirring and the solution was filtered with filter press to obtain afford 850 liter of the 2nd distillate of rice husk (designated as “RH-2” hereinafter).

1-3. 3rd purified distillate

3% (v/v) hydrogen oxide was added to the 2nd purified distillate obtained from step 1-2 and heated with stirring and the solution was filtered with filter press to obtain afford 700 liter of the 3rd distillate of rice husk (designated as “RH-3” hereinafter).

Example 2. . The preparation of combined herb extract

5g of dried leaf of Perilla frutescens var. acuta, and 5g of Artemisia princeps var orientalis were sliced into small pieces. The pieces were mixed with 100ml of the 1st purified distillate of rice husk. The mixture was added to water and extract at 85℃ for 24 hours. The extract was is filtered and concentrated at 60℃ under reduced pressure, concentrated and then dried by freeze drying to obtain the 90 ml of distillate combined with Perilla frutescens var. acuta (designated as “JSY-RH” hereinafter) and 90ml of that of Artemisia princeps var orientalis (designated as “SS-WA” hereinafter), which are used as test sample in following experiments. (See Fig.1)

Experimental Example 1. Anti-oxidative activity test.

In order to determine the anti-oxidative activity of inventive extract, following experiment was performed according to the procedure disclosed in the literature well-known in the art.

1-1. DPPH radical scavenging activity

The scavenging activity of the inventive distillates of the present invention on DPPH (1,1-dipheny-2-picrylhydrazyl) was determined as follows

(Blois, M.S. (1958) Antioxidant determination by the use of a stable free radical, Nature 181, 1199-1201)

800 microliter of DPPH solution dissolving 200 microgram of test samples and 100 microM DPPH in ethanol was mixed and left alone in shadow for 30 mins. BHA (Butylated hydroxyanisol), a potent anti-oxidant agent, was used as positive control. The absorbance was determined at 517 nm using by UV spectrophotometer (UV-1800, Shimadzu Co., Kyoto, JAPAN) according to following Math Figure 1.

MathFigure 1

Abs: Absorbance of DPPH solution with sample at 517 nm

Abc: Absorbance of DPPH solution without sample at 517 nm

As can be seen in Fig. 2, the inventive distillates showed potent scavenging activity on DPPH radical in a dose dependent manner and equivalent effect comparing with positive control. Accordingly, it has been confirmed that the inventive distillates itself have potent anti-oxidant activity.

1-2. FRAP (ferric ion reducing antioxidant power) scavenging activity

The scavenging activity of the inventive distillate or extract of the present invention on FRAP assay was determined according to the modified method in the literature as follows (Benzie, I.F.F. and Stranin, J.J. (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant powder : the FRAP assay. Anal. Biochem., 239, 70-76):

Acetate buffer (pH 3.6, 23mM) was prepared by using C₂H₃NaO₂and acetic acid (C₂H₄O₂). 10mM TPTZ solution was prepared by using TPTZ (2,4,6-tripyridyl-s-triazine). Cocktail solution consisting of the acetate buffer, 10 mM TPTZ solution and 20mM FeCl₃·6H₂O (10:1:1) was prepared and used at 37 ℃ during the following experiment. 30 microliter of each test sample was mixed with 1mL of the cocktail solution in Cubit cell and left alone in shadow for 30 mins. The absorbance was determined at 593 nm using by Victor3 1420 multi-label counter (Perkin Elmer Inc., Boston, MA, USA). The reducing activity was transformed into ferrous sulphate equivalent value (micromole) calculated from simple regression analysis result of absorbance according to the concentration of ferrous sulphate in each experiment.

As can be seen in Fig. 3, the inventive distillate (RH-1) and distillate combined with Perilla frutescens var. acuta (JSY-RH) and Artemisia princeps var orientalis (SS-WA) showed potent reducing activity on FRAP in a dose dependent manner. Accordingly, it has been confirmed that the inventive distillates have potent anti-oxidant activity.

1-3. DPPH anti-oxidant activity

The anti-oxidant activity of the inventive distillates of the present invention on DPPH (1,1-dipheny-2-picrylhydrazyl) was determined as follows(Blois, M.S. (1958) Antioxidant determination by the use of a stable free radical, Nature 181, 1199-1201):

The electron-donating ability of each sample to DPPH was determined for verifying its reducing power. 0.5 ml of 0.4 mM DPPH solution was added to 1ml of various concentrations of each sample and mixed with vortex for 10 seconds. The solution was reacted for 30 mins at 37 ℃ and the absorbance of the solution was determined at 517 nm using by microplate reader.

As can be seen in Fig. 4, various concentrations of RH-1 distillates, showed potent anti-oxidant activity on DPPH, i.e., 24% (1 microliter/well), 71% (3 microliter/well), and 82% (10 microliter/well). Various concentrations of JSY-RH distillates, showed potent anti-oxidant activity on DPPH, i.e., 27% (1 microliter/well), 61% (3 microliter/well), and 82% (10 microliter/well) and those of SS-WA distillates, showed potent anti-oxidant activity on DPPH, i.e., 20% (1 microliter/well), 64% (3 microliter/well), and 79% (10 microliter/well). Accordingly, it has been confirmed that the inventive distillates have potent anti-oxidant activity.

1-4. SOD-like activity

The scavenging activity of the inventive distillate or extract of the present invention on SOD-like activity was determined according to the modified method in the literature as follows (Marklund, S. and G. Marklund. 1975. Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 47 : 468-474.):

3ml of Tris-HCl buffer (50 mM Tris[hydroxymethyl] aminomethane+10mM EDTA, PH 8.5) and 0.2ml of 7.2 mM pyrogallol were added to 0.2 ml of each sample and left alone at 37 ℃ for 10 mins. The reaction was stopped by adding 1ml of 1N HCl and the absorbance of the solution was determined at 517 nm using by microplate reader.

As can be seen in Fig. 5, the inventive distillates, i.e., RH-2 and RH-3 showed potent SOD-like activity scavenging effect in a dose dependent manner, and especially, 100ml/ml of test samples showed almost 100% inhibitory activity. Accordingly, it has been confirmed that the inventive distillates have potent anti-oxidant activity.

1-5. Xanthine oxidase inhibition

The inhibitory activity of the inventive distillate or extract of the present invention on xanthine oxidase enzyme activity was determined according to the modified method in the literature as follows (Stirpe, F. and Della Corte E. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase(type D) to oxidase(type O). J Biol Chem. 244(14): 3855-3863.):

0.2ml of substrate solution dissolving 2mM xanthine in 0.6 ml of 0.1M potassium phosphate buffer (pH 7.5) was added to 0.2 ml of each sample and 0.1 ml of 0.2 unit/ml of xanthine oxidase was added thereto to react together at 37 ℃ for 5 mins. The reaction was stopped by adding 1ml of 1N HCl and the absorbance of uric acid formed from the reaction was determined at 292 nm using by microplate reader. Inhibitory activity of xanthine oxidase was determined by the decreasing ratio of the group with test sample and that without test sample.

As can be seen in Fig. 6, the inventive distillates, i.e., RH-2 and RH-3 showed potent scavenging effect on superoxide radical in a dose dependent manner, and equivalent effect comparing with positive control (BHA). Accordingly, it has been confirmed that the inventive distillates have potent anti-oxidant activity.

Experimental Example 2. Skin anti-aging activity test.

In order to determine the anti-aging activity of inventive distillates, following experiment was performed according to the procedure disclosed in the literature well-known in the art (J. Appl. Biol. Chem., 52(4): pp174-179, 2005).

2-1. Collagenase enzyme inhibition

The inhibitory activity of the inventive distillate or extract of the present invention on collagenase enzyme activity was determined according to the modified method in the literature as follows (Wunsch, E. and H. G. Heindrich. 1963. Zur quantitativen bestimmung der collagenase. Hoppe-Seyler's Physiol Chem. 333: 149-151.):

0.15 ml of collagenase (0.2 mg/ml) was added to the mixture of 0.25ml of substrate solution dissolving 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg (0.3mg/ml) and 0.1M Tris-HCl buffer (pH 7.5) added with 4 mM CaCl2 and left alone at room temperature for 20 mins. The reaction was stopped by adding 0.5ml of 6% citric acid and 1.5 ml of ethylacetate was added thereto. The absorbance of supernatant was determined at 320 nm using by microplate reader. Inhibitory activity of collagenase was determined by the decreasing ratio of the group with test sample and that without test sample.

As can be seen in Fig. 7, the inventive distillates, i.e., RH-2 and RH-3 showed potent inhibiting effect on collagenase activity in a dose dependent manner, and especially, 100ml/ml of test samples showed almost 100% inhibitory activity, which is more potent than that of positive control group treated with EGCG (epigallocatechin gallate) isolated from green tea. Accordingly, it has been confirmed that the inventive distillates have potent inhibiting activity from skin-aging and anti-wrinkle activity.

2-2. Elastase enzyme inhibition

The inhibitory activity of the inventive distillate or extract of the present invention on elastase enzyme activity was determined according to the modified method in the literature as follows (Cannell, R. J. P., S. J. Kellan, A. M. Owsianks, and J. M. Walker. 1988. Results of a large scale scrren of microalgae for the production of protease inhibitors. Planta Med. 54: 10-14.):

0.05ml of pancreatic solution (Type I: from porcine pancreas; 0.6 units/ml) was added to 0.1ml of various concentrations of each sample. 0.1 ml of mixture substrate solution dissolving N-succinyl-(L-Ala)3-p-nitroanilide (1mg/ml) in 50 mM Tris-HCl buffer (pH 8.6) and reacted together at 37 ℃ for 30 mins. The absorbance of the solution was determined at 410 nm using by microplate reader. Inhibitory activity of elastase enzyme was determined by the decreasing ratio of the group with test sample and that without test sample.

As can be seen in Fig. 8, the inventive distillates showed potent inhibiting effect on elastase activity in a dose dependent manner, and especially, 100ml/ml of test samples i.e., RH-2 and SS-WA showed 50% and 75% inhibitory activity respectively. Accordingly, it has been confirmed that the inventive distillates have potent inhibiting activity from skin-aging and anti-wrinkle activity.

Experimental Example 3. Anti-bacterial test.

In order to determine the anti-bacterial activity of inventive distillates, following experiment was performed according to the procedure disclosed in the literature well-known in the art (Korean J. Biotechnol. Bioeng., 19(5): pp381-384, 2004).

2 days before the test day, Staphylococcus aureus, a representative germ in atopic dermatitis disease, was inoculated into liquid medium and incubated at 37 ℃ and diluted to 1/100. 0.5 microliter of diluted solution was spread on LB agar growth medium and 40 microliter of various concentrations of test sample was added to 8mm paper dish. The dish was transferred to agar plate to incubate for 2 days and the size of growth inhibition region was determined.

As can be seen in Table 1, the inventive distillates, i.e., RH-2 and RH-3, showed potent inhibiting effect on the bacterial growth in a dose dependent manner, and especially, SS-WA showed most potent inhibitory activity. Accordingly, it has been confirmed that the inventive distillates have potent anti-bacterial activity.

Experimental Example 3. Anti-bacterial test.

As can be seen in Table 1, the inventive distillates, i.e., RH-2 and RH-3, showed potent inhibiting effect on the bacterial growth in a dose dependent manner, and especially, SS-WA showed most potent inhibitory activity. Accordingly, it has been confirmed that the inventive distillates have p

otent anti-bacterial activity.

Experimental Example 3. Anti-bacterial test.

Table 1

Species	Treatment	Clear zone (mm)
Staphylococcus aureus	Control (water)	0
	RH-1	2
	RH-2	5
	RH-3	5
	JSY-RH	3
	SS-WA	6

Hereinafter, the formulating methods and kinds of excipients will be described, but the present invention is not limited to them. The representative preparation examples were described as follows.

Preparation Example 1. Preparation of inventive skin lotion composition

The inventive skin lotion composition comprising inventive distillate was prepared according to following table.

Table 2

No	Composition	(w %)
1	Inventive Distillate (RH-3)	1.0
2	Butylene glycol	5.0
3	Glycerin	3.0
4	Carboxyvinyl polymer	0.06
5	Disodium ethylene diamine tetra acetic acid	0.01
6	Ethanol	7.0
7	Sorbitan mono stearate	0.8
8	Triethanolamine	0.2
9	Hydrogenated castor oil	0.4
10	Phenyltrmethicone	0.5
11	Propyl paraoxybenzoate	0.2
12	Methyl paraozybenzoate	0.2
13	Flavoring agent	0.1
14	Distilled water	Optimum
	Total	100.0

Preparation Example 2. Preparation of inventive nutrition lotion composition (1)

The inventive nutrient lotion composition comprising inventive distillate was prepared according to following table.

Table 3

No	Composition	(w %)
1	Inventive Distillate (RH-2)	1.0
2	Stearic acid	1.0
3	Cetyl alcohol	1.0
4	Glycerylmonostearate	1.0
5	Sorbitan stearate	1.0
6	Polyoxyethylene sorbitan monostearate	0.5
7	Liquid paraffin	4.0
8	Squalene	3.0
9	Caprilic/ Capric triglyceride	4.0
10	Carboxy vinyl polymer	0.1
11	Butylene glycol	5.0
12	Triethanol amine	0.2
13	Propyl paraoxybenzoate	0.2
14	Methyl paraoxybenzoate	0.2
15	Flavoring agent	0.1
16	Distilled water	Optimum
	Total	100.0

Preparation Example 3. Preparation of inventive nutrition lotion composition (2)

Table 4

No	Composition	Example 4(w %)
1	Inventive Distillate (RH-1)	1.0
2	Stearic acid	1.0
3	Cetyl alcohol	1.0
4	Glycerylmonostearate	1.0
5	Sorbitan stearate	1.0
6	Polyoxyethylene sorbitan monostearate	0.5
7	cetearex-12	1.0
8	wax	4.0
9	Liquid paraffin	4.0
10	Squalene	4.0
11	Caprilic/ Capric triglyceride	4.0
12	Carboxy vinyl polymer	0.3
13	Butylene glycol	5.0
14	Glycerin	3.0
15	Triethanol amine	0.5
16	Propyl paraoxybenzoate	0.2
17	Methyl paraoxybenzoate	0.2
18	Flavoring agent	0.1
19	Distilled water	Optimum
	Total	100.0

Preparation Example 4. Preparation of inventive massage cream composition

The inventive massage cream composition comprising inventive distillate was prepared according to following table.

Table 5

No	Composition	(w %)
1	Inventive Distillate (SS-WA)	1.0
2	Stearic acid	0.5
3	Glycerylmonostearate	1.0
4	Polysorbate 60	1.5
5	Sorbitan sesquiolate	0.8
6	Liquid paraffin	40.0
7	Squalene	5.0
8	Caprilic/ Capric triglyceride	4.0
9	Butylene glycol	3.0
10	Triethanol amine	0.2
11	Carboxy vinyl polymer	0.3
12	Propyl paraoxybenzoate	0.2
13	Methyl paraoxybenzoate	0.2
14	Flavoring agent	0.1
15	Distilled water	Optimum
	Total	100.0

Preparation Example 5. Preparation of inventive essence composition

The inventive essence composition comprising inventive distillate was prepared according to following table.

Table 6

No	Composition	(w %)
1	Inventive Distillate (JSY-RH)	1.0
2	Phenyltrimethicone	1.0
3	Polyoxyethylene hydrogenated castor oil	1.0
4	Glycerypolyacrylate/dimethicone/cyclomethicone	3.0
5	Ethanol	3.0
6	Butyleneglycol	4.0
7	Glycerin	3.0
8	Carboxy vinyl polymer	0.2
9	Xanthan gum	0.2
10	Propyl paraoxybenzoate	0.2
11	Methyl paraoxybenzoate	0.2
12	Flavoring agent	0.1
13	Distilled water	Optimum
	Total	100.0

Preparation Example 6. Preparation of hydrophilic ointment composition

The inventive hydrophilic ointment composition comprising inventive distillate was prepared according to following table.

Table 7

No	Composition	(w %)
1	Inventive Distillate (RH-3)	0.5	-
2	Inventive Distillate (JSY-WA)	-	0.5
3	White Vaseline	250	250
4	Stearyl alcohol	220	220
5	Ethyl (or methyl) p-oxybenzoate	0.25	0.25
6	Propylene glycol	120	120
7	Sodium Lauryl sulfate	15	15
8	Propyl p-oxybenzoate	0.15	0.15

The invention being thus described, it will be obvious that the same may be varied in many ways. Such variations are not to be regarded as a departure from the spirit and scope of the present invention, and all such modifications as would be obvious to one skilled in the art are intended to be included within the scope of the following claims.

As described in the present invention, present invention provides a compos

ition comprising a purified distillate of rice husk showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient and that the distillate shows potent anti anti-oxidative, anti-bacterial and anti-wrinkle activity through various experiments, therefore, it can be used as the effective and safe composition for treating and preventing skin aging.

Claims

A composition comprising a purified distillate of rice husk showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient.
The composition according to claim 1, wherein said composition is topical pharmaceutical composition or cosmetic composition.
The composition according to claim 1, wherein said purified distillate of rice husk is the 1st purified distillate of rice husk, the 2nd purified distillate of rice husk or the 3rd purified distillate of rice husk.
The composition according to claim 3, wherein said 1st purified distillate of rice husk is prepared by the process comprising the steps: subjecting dried rice husk to carbonization with carbonizing machine at the temperature ranging from 200 ℃ to 800 ℃ for the period ranging from 10 mins to 200 mins and condensing the discharged smoke from carbonization treatment to afford crude vinegar distillate of rice husk at 1st step; subjecting to distillation under reduced pressure at low temperature at the 2nd step to afford the 1st distillate of rice husk.
The composition according to claim 3, wherein said 2nd purified distillate of rice husk is prepared by the process comprising the steps: adding active carbon to the 1st purified distillate of rice husk, heating with stirring and filtering with filter press to obtain the 2nd purified distillate of rice husk.
The composition according to claim 3, wherein said 3rd purified distillate of rice husk is prepared by the process comprising the steps: adding oxides to the 2nd purified distillate of rice husk, heating with stirring and filtering with filter press to obtain the 3rd purified distillate.
A composition comprising a purified distillate of rice husk and at least one herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne showing potent anti-oxidative, anti-bacterial and anti-wrinkle activity as an active ingredient.
The composition according to claim 7, wherein said composition is topical pharmaceutical composition or cosmetic composition.
The composition according to claim 7, wherein said herb and the purified distillate of rice husk are mixed with a mixed ratio of 1: 0.0001-1 (v/w).
The composition according to claim 7, wherein said, Perilla frutescens var. acuta, and Artemisia princeps var orientalis is mixed with a mixed ratio of 1: 0.01-50 (w/w).
The composition according to claim 2 or 8, wherein said topical pharmaceutical composition is cream, gel, liniment, ointment, lotion, gel, balm, patch, paste, spray solution, cataplasma, or aerosol.
The composition according to claim 2 or 8, wherein said cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, nutrient cream, moisture cream, hand cream, massage cream, essence, pack, foundation, cleansing water, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, soap, treatment or beauty solution.
A use of a purified distillate of rice husk for the preparation of therapeutic agent for the treatment and prevention of skin aging in mammal or human.
A use of a purified distillate of rice husk and at least one herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne for the preparation of therapeutic agent for the treatment and prevention of skin aging in mammal or human.
A method of treating or preventing skin aging in mammal or human in need thereof comprising administering topically to said mammal or human with an effective amount of a purified distillate of rice husk, together with a pharmaceutically acceptable carrier thereof.
A method of treating or preventing skin aging in mammal or human in need thereof comprising administering topically to said mammal or human with an effective amount of the a purified distillate of rice husk and at least one herb selected from the group consisting of Perilla frutescens var. acuta, Artemisia princeps var orientalis, Cudrania tricuspidata (Carr.) Bureau ex Lavallee, and Chaenomeles sinensis Koehne, together with a pharmaceutically acceptable carrier thereof.