WO2014119826A1 - Cosmetic composition for whitening or wrinkle improvement which includes seaweed extract as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for whitening or wrinkle improvement which includes a seaweed extract as an active ingredient. The seaweed extract according to the present invention has excellent tyrosinase inhibitory activity, melanogenesis inhibitory activity, collagen synthesis ability and MMP inhibitory activity, shows almost no cytotoxicity in keratinocytes (HaCaT), human dermal fibroblast cells (1064 SK) and epidermoid carcinoma cells (B16F), is non-irritant, shows very low sensitization (level I), and does not provide phototoxic properties and photosensitive properties. Accordingly, the seaweed extract according to the present invention is useful for a cosmetic composition for whitening or wrinkle improvement.

Description

Cosmetic composition for whitening or improving wrinkles containing seaweed extract as an active ingredient

The present invention relates to a cosmetic composition for improving whitening or wrinkles containing a seaweed extract as an active ingredient.

Skin that is constantly exposed to sunlight has a problem of being injured by UV and changing skin color. Pigmentation is an important cause of such skin color change.

In general, pigments affecting skin color include melanin, melanoid, carotene, hemoglobin, and the most important pigment is melanin.

Melanin plays an important role in preventing skin damage from ultraviolet rays by absorbing or scattering ultraviolet rays, and also excellent in removing free radical species, but sometimes melanin itself generates free radicals, and catechol in melanin structure Or quinones reduce or oxidize other substances, and melanin itself exhibits the properties of free radicals. Therefore, in order to prevent skin discoloration, it is necessary to suppress melanin production.

Melanin is biosynthesized into a brown, eumelanin polymer through a series of oxidation processes beginning with the conversion of tyrosine, an amino acid, to tyrosinase and conversion to dihydroxy phenylalanine. The biosynthesis process of melanin proceeds in the organelle of a special type of brown cells called melanosomes, and through the biosynthesis process, melanosomes including melanin granules migrate around the nuclei of keratinocytes and accumulate.

The synthesis of melanin, the number of melanosomes, and the migration to the surrounding keratinocytes are largely genetically influenced, in part, by hormones and ultraviolet rays, and other intracellular regulatory factors such as cytokines, copper, zinc, It is known to be affected by metal ions such as iron and interferon, prostaglandin, histamine and the like.

Conventionally, ascorbic acid, hydroquinone, kojic acid, arbutin and some extracts are used as whitening cosmetics because they have inhibitory activity of tyrosinase involved in melanogenesis, but they are degraded due to their low stability in cosmetic formulations or Its use is limited due to problems such as the occurrence of off-flavor, unclear efficacy at the biological level. Therefore, in recent years, researches are being actively conducted to develop skin whitening ingredients derived from natural products which can minimize side effects on the skin.

In addition, aging of the skin may be divided into stress, disease, environmental factors, wounds, or internal aging that occurs with age, and light aging caused by exposure to light such as ultraviolet rays.

Aging of the skin is caused by the destruction of the antioxidant protective film present in the living body by the activated free radicals, thereby oxidizing lipids, proteins, polysaccharides and nucleic acids, which are the major constituents of the skin, and thus destroying skin cells and tissues.

In particular, when the protein is oxidized, collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are the connective tissues of the skin, are cleaved, which causes severe excessive inflammatory reaction and skin elasticity. If this becomes more severe, mutations in the DNA can lead to mutations, cancer, and reduced immune function.

Therefore, in order to prevent skin aging, it is necessary to prevent skin damage by eliminating free radicals caused by various causes, and also to restore skin by regenerating and proliferating through active metabolism. have.

In addition, skin aging may be promoted by matrix metalloproteinase (MMP), a collagen degrading enzyme. That is, in vivo, the synthesis and degradation of extracellular matrix such as collagen is properly regulated, but as aging progresses, collagen synthesis decreases and the expression of collagen degrading enzyme MMP is promoted, resulting in decreased skin elasticity and wrinkles. .

Therefore, in order to prevent skin aging and wrinkle generation, it is necessary to control or inhibit MMP expression in which activation is induced in cells. Furthermore, it is necessary to enhance collagen synthesis in order to improve skin wrinkles.

Adenosine, retinoic acid, and the like are known to be effective for improving skin wrinkles, but adenosine is ineffective in clinical practice, and retinoic acid can be used in women of childbearing potential, and there is a high risk of irritation such as erythema.

On the other hand, Laver (Raver) is a red algae belonging to the genus (Porphyra) is called haekai or Haitai. The fronds are embedded in a thin and sticky material, and have various colors ranging from dark brown to reddish-pink. The sexual reproduction occurs at the edge. Laver grows in the upper intertidal zones of the southern and northern hemispheres and grows well in nitrogen-rich water, such as areas where sewage drains. The seaweed is harvested, dried and used for food, more than any other sea horse. About 70 kinds of seaweed are known worldwide, but about 10 species are known in Korea. Among them, P. tenera and P. yezoensis are farmed. In addition, P. kuniedai, P. suboriculata, and P. seriata It may also grow a little between the seaweeds grown. Laver is rich in protein and dietary fiber, and is popular in cosmetics. N-nitrosodimethylamine (N-N) has the effect of increasing nitrite scavenging effect, collagen synthesis and production of pyridinoline, which is a maturation crosslink. -nitrosodimethylamine) has been reported to inhibit the production of. In addition, porphyran isolated from seaweed has been studied to regulate the effects of calcium, magnesium and potassium in hyperlipidemic mice, and has been used to treat hemorrhoids and ovulation.

As mentioned above, although various pharmacological effects of laver have been reported, studies on the whitening or wrinkle improvement effect of laver are insignificant.

Therefore, there is an urgent need for research and development on whitening or wrinkle improvement using seaweed extract.

The present inventors, while studying the whitening or wrinkle improvement using the seaweed extract, confirmed that the seaweed extract shows excellent tyrosinase activity inhibitory activity, melanin synthesis inhibitory ability, collagen synthesis ability and MMP activity inhibition ability, and completed the present invention.

Therefore, the present invention is to provide a cosmetic composition for improving whitening or wrinkles containing the seaweed extract as an active ingredient.

The present invention provides a cosmetic composition for whitening or improving wrinkles containing the seaweed extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The seaweed extract as an active ingredient in the cosmetic composition of the present invention can be obtained by a conventional extraction method, and commercially available ones can be purchased and used. Conventional extraction methods may include, but are not limited to, ultrasonic extraction, filtration and reflux extraction. In the present invention, the extract was prepared using the method described in the Republic of Korea Patent Publication No. 10-969325 filed by the applicant.

Specifically, the dried steam is added to water, C ₁ ~ C ₄ alcohol or a mixed solvent of water and C ₁ ~ C ₄ alcohol and maintained for 1 to 5 hours in a constant temperature water bath maintained at 30 ~ 60 ℃ to extract the active ingredient do. The C ₁ ~ C ₄ alcohol may be selected from 1 to 100% methanol or 1 to 100% ethanol, preferably 1 to 100% methanol. Thereafter, the extract was filtered and the filtrate was concentrated in vacuo to obtain a seaweed extract in powder form. Seaweed extract in powder form is added to distilled water, filtered to remove impurities, and purified to obtain a seaweed extract.

The laver used in the present invention is preferably radiation pattern laver (Porphyra yezoensis), but is not limited thereto.

Seaweed extract according to the present invention is excellent in inhibiting tyrosinase activity, melanin synthesis inhibitory ability, collagen synthesis and MMP activity, almost cytotoxic in keratinocytes (HaCaT), human fibroblasts (1064 SK) and skin cancer cells (B16F) It is non-irritant, nonsensitizing, very sensitive (grade I), and does not cause phototoxicity and photosensitization. Therefore, the seaweed extract according to the present invention can be usefully used in the cosmetic composition for whitening or wrinkle improvement.

Seaweed extract of the present invention may be included in 0.0001 to 30.0% by weight, more preferably 0.001 to 10.0% by weight, and most preferably 0.01 to 5.0% by weight relative to the total weight of the cosmetic composition.

The cosmetic composition according to the present invention contains components conventionally used in cosmetic compositions in addition to the seaweed extract as an active ingredient, and includes, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. Include.

Cosmetic compositions according to the invention can be prepared in any formulations conventionally prepared in the art, for example solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, cleansing, oils, Powder foundations, emulsion foundations, wax foundations and sprays and the like can be formulated, but is not limited thereto.

More specifically, the cosmetic composition according to the present invention can be prepared in the form of a flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder have.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular, in the case of a spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as the carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan are used.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

If the formulation of the invention is a cleansing, in particular a surfactant-containing cleansing, it is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, methyltaurate, sarcosy as a carrier component. Nates, fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Seaweed extract according to the present invention is excellent in inhibiting tyrosinase activity, melanin synthesis inhibitory ability, collagen synthesis and MMP activity, almost cytotoxic in keratinocytes (HaCaT), human fibroblasts (1064 SK) and skin cancer cells (B16F) It is non-irritant, nonsensitizing, very sensitive (grade I), and does not cause phototoxicity and photosensitization.

1 is a diagram showing the effect of the seaweed extract of the present invention on the inhibition of tyrosinase activity.

Figure 2 is a diagram showing the effect of the laver extract of the present invention on the inhibition of melanin activity.

Figure 3 is a view showing the effect of the extract of the present invention on the cell proliferation of keratinocytes (HaCaT), human fibroblasts (1064 SK), skin cancer cells (B16F).

Figure 4 is a diagram showing the collagen synthesis ability of the seaweed extract of the present invention.

5 is a diagram showing the inhibitory activity of MMPs activity of the seaweed extract of the present invention.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Preparation Example 1  : Preparation of Seaweed Extract

The seaweed extract used in the present invention was prepared using the method described in Korean Patent Publication No. 10-969325 to which the applicant has applied for and registered.

(1) Preparation of crude extract (PE)

A constant temperature water bath maintained at 45 ° C after adding 100 g of dried Porphyra yezoensis to a vial, followed by adding 20 ml of 80% by volume aqueous methanol solution (mixed solvent consisting of 80 volumes of methanol and 20 volumes of water). The active ingredient was extracted by maintaining for 3 hours. The contents in the vial were then filtered through filter paper (whatman No. 2) to separate the supernatant from the remaining solids. Extraction was carried out two more times by extraction and filtration from the remaining solid. The separated supernatants were collected, concentrated in vacuo, concentrated and filtered (whatman No. 2), and the remaining solids were extracted twice in the same manner. The extracts thus obtained were collected, concentrated in a vacuum evaporator and dried to give a crude extract (Pophyra extract, PE) in powder form.

(2) Preparation of Crude Extract Fractions

10 ml of the crude extract in powder form obtained in (1) was added to 90 ml of distilled water and dissolved to prepare an aqueous crude extract solution, which was then filtered through a membrane filter (Gelman, USA) having a pore size of 0.45 nm to remove impurities. . The crude extract aqueous solution from which impurities are removed is composed of a water-methanol concentration gradient (previously water and then 50 vol% methanol) on a Prep LC system (dual pump (Koto, Japan), auto injector, UV / RI detector, and fraction collector). Aqueous extract) (developing rate of 5ml / min) and then using a UV detector and RI detector to obtain a crude extract fraction absorbing the ultraviolet wavelength of 280 ~ 400 nm.

(3) Preparation of Tablet Extract

The crude extract fraction obtained in (2) was 10ml / min with 0.2% by volume of acetic acid aqueous solution in a hydrophobic cross column (Capcellpak C18 UG120 semi-prepatative column; Shiseido, Japan) protected by UG120 guard column (Shiseido, Japan). It was developed at the rate of and purified first. The first purified extract was purified on a size exclusion column (Ohpak 2002; Shiseido, Japan) with water at a rate of 2.5 mL / min and secondarily purified. The secondary tablet extract was a bright yellow liquid, which was concentrated on a vacuum evaporator and lyophilized to give the tablet extract in powder form.

Example 1  : Whitening Effect of Seaweed Extract of the Present Invention

In order to confirm the whitening efficacy of the seaweed extract of the present invention, the following experiment was performed.

1. Inhibitory activity of tyrosinase activity

Seaweed extract prepared in Preparation Example 1 (5, 10, 20, 30 μl) in a reaction solution containing 0.1 M phosphate buffer (pH 6.8), 50 mM L-tyrosine, mushroom tyrosinase (0.5 U / μl) , 28Brix) was added and reacted at 37 ° C. for 20 minutes, and the absorbance was measured at 475 nm. The tyrosinase activity inhibition rate was calculated by the following formula. 2% arbutin was used as a control. The results are shown in FIG.

※ Inhibition rate of tyrosinase activity (%) = [(A-B) / A] x 100

A: absorbance after the reaction of the reaction solution containing no seaweed extract

B: Absorbance after Reaction of Reaction Solution Containing Seaweed Extract

As shown in Figure 1, the laver extract of the present invention was confirmed to have excellent tyrosinase activity inhibitory ability.

2. Inhibition of Melanin Synthesis

Inoculate B16F10 cells to 5 × 10 ⁴ cells / ml per well in a 24 well plate and incubate for 24 hours in DMEM medium containing 10% FBS, and extract of seaweed prepared in Preparation Example 1 (10, 20, 30 μl) Was added and incubated for 30 minutes. Then, after incubation for 3 days with the addition of α-MSLAN (α-melanocyte stimulate hormone), the melanin was dissolved for 3 hours at 60 ℃ in 10% DMSO / 1N NaOH in centrifuged cells to measure the absorbance at 490 nm The amount of melanin synthesis was examined.

The results are shown in FIG.

As shown in Figure 2, the laver extract of the present invention was confirmed to inhibit melanin synthesis by α-MSH close to 80% in B16F10 cells. Therefore, it can be seen that the seaweed extract of the present invention is excellent in melanin synthesis inhibitory ability.

Example 2  : Wrinkle improvement effect of the seaweed extract of the present invention

In order to confirm the wrinkle improvement effect of the seaweed extract of the present invention, the following experiment was performed.

1. Cell proliferation against keratinocytes (HaCaT), human fibroblasts (1064 SK), skin cancer cells (B16F)

Thiazolyl Blue Tetrazolium Bromide (MMT) assay measured cell proliferation of keratinocytes (HaCaT), human fibroblast primary cell line (1064 SK) and skin cancer cells (B16F). That is, the growth of the cells was determined by measuring the purple formazan produced by the reduction of MTT by living cells. After irradiating each cell with UV, the culture medium was again exchanged with DMEM medium containing 10% FBS and antibiotics and incubated for another 8 hours. 10 μl of MTT (5 mg / mL PBS) solution was then added to each well and incubated for 3 hours. The culture solution containing MTT was removed, 200 μl of DMSO was added to each well, extracted for 30 minutes, and the absorbance was measured at 560 nm.

The results are shown in FIG.

As shown in Figure 3, the laver extract of the present invention showed no cytotoxicity in keratinocytes (HaCaT), human fibroblasts (1064 SK), skin cancer cells (B16F).

2. Collagen Synthesis

Human fibroblasts (1064 SK) were aliquoted into 500 μl in 24 well plates at 2 × 10 ⁵ cells / ml and incubated for 24 hours. After incubation, all of the medium was removed, and the medium (serum extract of Preparation Example 1) was diluted to a concentration (10, 20, 30 μl) selected through the evaluation of cell proliferation ability and added to each well using a medium containing no serum. After incubation for 48 hours, the medium was collected, and the supernatant was taken by centrifugation for 2000 rpm / 3 minutes. Collagen synthesis capacity was measured using the supernatant by the procollagen type 1 c-peptide ELISA method.

The procollagen type 1 c-peptide ELISA method is as follows.

100 μl of antibody-POD conjugate solution was added to each well of a 96 well plate coated with antibody titer, and 20 μl of the culture solution and the standard solution diluted to 1/5 were added. The standard solution was prepared by diluting the collagen standard to 0, 10, 20, 40, 80, 160, 320, 640 ng / ml with diluent, respectively. After sealing a plate, it was made to react at 37 degreeC for 3 hours. After the reaction, each well was washed 3-4 times with PBS. 100 μl of substrate solution was added thereto, followed by reaction at room temperature for 15 minutes. 100 μl of stop solution (1N H ₂ SO ₄ or 1N HCl) was added thereto, and the absorbance was measured at 450 nm using an ELISA reader. The amount of collagen measured was corrected after protein quantification.

The results are shown in FIG.

As shown in FIG. 4, the laver extract of the present invention was confirmed to have excellent collagen synthesis ability.

3. MMPs activity inhibition

Gelatin zymography was performed using human skin keratinocytes (HaCaT) medium solution obtained to measure the collagen synthesis ability of 2, and the degree of inhibition of activity of MMP2 and MMP9 was confirmed.

The results are shown in FIG.

As shown in FIG. 5, the laver extract of the present invention showed a slight inhibitory activity of about 10% on MMP2 activity in keratinocytes (HaCaT), while inhibiting the activity of MMP9 by about 40%.

Example 3  : Oral Administration Toxicity Test of Seaweed Extract of the Present Invention

According to "Toxicity Test Standards for Drugs," a single oral dose toxicity test was conducted for 14 days after the oral dose was administered once for each male or female, using the SD rat dose set at 2000 mg / kg BW. , General symptoms, weight change and autopsy findings were observed.

As a result, no dead animals and abnormal findings were observed during the test period. In addition, there was no statistical difference between the groups. Autopsy of all live animals revealed no abnormalities associated with the administration of test substance.

From the above results, the toxicological findings related to the administration of the test substance were not recognized in a single oral administration of the laver extract 2000 mg / kg B.W. of the present invention to SD rats, so the approximate lethal dose was 2000 mg / kg B.W. It is judged as above.

Example 4  : Skin irritation test of seaweed extract of the present invention

In order to evaluate the skin irritation of the laver extract of the present invention for NZW rabbits, the test substance was applied to the skin of rabbits for 24 hours, and then mortality, general symptoms, weight change and skin irritation for 72 hours were evaluated.

As a result of the evaluation, no dead animals were observed due to the application of the test substance during the test period, and no abnormal symptoms were observed in all the test animals. In addition, weight measurements showed normal weight gain in all animals. As a result of observing skin irritation after application of test substance, erythema and edema were not observed, and the primary skin irritation index (P.I.I.) was calculated as "0.0" according to the method of calculating Draize.

From the above results, it is determined that the test substance (seaweed extract) is a non-irritating substance in the skin irritation test for NZW rabbits.

Example 5  : Skin Sensitization Test of Seaweed Extract of the Present Invention

The skin sensitization of the laver extract of the present invention against guinea pigs was evaluated by the Buehler method, and mortality, general symptoms, weight change and post-induced skin response were examined during the test period.

As a result, no dead animals were observed in the test substance administration group and the negative control group during the test period, and no unusual abnormality due to the application of the test substance was observed. In addition, as a result of weight measurement, all test animals of the test substance-administered group showed normal weight gain. As a result of evaluating the skin reaction at 24 and 48 hours after causing the test substance to 100% concentration, the sensitization index (average skin response score) and frequency index (sensitization rate) in the test substance-treated group were "0.0" and "0.0", respectively. % "," 0.0 "and" 0.0% "were calculated.

From the above results, no skin reaction was observed during the Buehler method evaluation of the skin sensitization of the seaweed extract of the present invention against guinea pigs, so this test substance (seaweed extract) was a very sensitive substance by the evaluation criteria (grade I). Was evaluated.

Example 6  : Phototoxicity Test of Seaweed Extract of the Present Invention

To investigate the phototoxicity of the laver extract of the present invention against guinea pigs, six guinea pigs were coated with laver extracts for UV irradiation, followed by 72 hours of mortality, general symptoms and weight changes and 24, 48 and 72 hours. The first skin reaction was evaluated.

As a result of the evaluation, no dead animals were observed during the test period, and no unusual abnormalities due to the application of the test substance were observed. In addition, as a result of weight measurement during the test period, all the test animals showed an overall weight gain. After the test material was open-patched to the test material treatment compartment at 100% concentration for 30 minutes, UVA (320-420 nm) was irradiated with a light amount of 15 J / cm 2 for a predetermined time to detect skin reactions of the UVA irradiated and non-irradiated areas. As observed at 24, 48 and 72 hours, no skin reaction was observed and the phototoxic index was evaluated as "0.0". For the compartments treated with the positive control, the phototoxicity index was evaluated as "2.2", "1.0" and "0.2" at 24, 48 and 72 hours, and was evaluated as mildly irritating substance.

From the above results, when the phototoxicity of the seaweed extract of the present invention against guinea pigs was evaluated by irradiation with UV, it did not cause skin reactions such as erythema and edema, so the test substance (seaweed extract) was a material that does not cause phototoxicity. Was evaluated.

Example 7  : Photosensitization Test of Seaweed Extract of the Present Invention

In order to investigate the photo-allergic index of the seaweed extract of the present invention against guinea pigs, the guinea pigs were coated with a seaweed extract and irradiated with UV, followed by mortality, general symptoms, weight change and sensitization of the test animals. The skin reaction was investigated.

As a result, no dead animals were observed in all test groups during the test period, and no unusual abnormalities due to application of the test substance were observed. In addition, as a result of weight measurement, all test animals of the test substance-administered group showed normal weight gain. After the test material was open-patched to the test material treatment compartment at 100% concentration for 2 hours, UVA (320-420 nm) was irradiated with a light amount of 10 J / cm 2 for a predetermined time to skin reactions of the UVA irradiated and non-irradiated areas. Was observed at 24, 48 and 72 hours, and no skin reaction was observed in the test substance-administered group and the negative control group. In the positive control group, skin reactions were observed at 24, 48 and 72 hours, and the photosensitization index was evaluated as "1.8", "1.2" and "0.6", respectively.

From the above results, when the photosensitization of the seaweed extract of the present invention to guinea pigs was evaluated by irradiation with UV, it did not cause skin reactions such as erythema and edema, so the test substance (steam extract) did not cause photosensitization. It was evaluated as a substance.

Formulation example  : Preparation of Cosmetic Formulation

1. Flexible Lotion

Seaweed extract 0.5% by weight

Glycerin 5.0 wt%

1,3-butylene glycol 3.0 wt%

Ethanol 5.0 wt%

0.5% by weight of polyoxyethylene nonylphenyl ether

Spices

Preservative

Purified water level

2. Converging lotion

Seaweed extract 0.5% by weight

Glycerin 3.0 wt%

Citric Acid 0.1 wt%

Ethanol 10.0 wt%

1.0% by weight of polyoxyethylene oleyl ether

Sorbitol 2.0 wt%

Spices

Preservative

Purified water level

3. Lotion (Emulsion)

Seaweed extract 0.5% by weight

Glycerin 3.0 wt%

1,3-butylene glycol 8.0% by weight

Squalane 10.0 wt%

2.0% by weight of polyoxyethylene sorbitan monooleate

1.5% by weight of triethanolamine

Glyceryl Stearate 0.5 wt%

0.2% by weight of stearyl glycyrrhetinate

Carboxy vinyl polymer 0.1 wt%

Arginine 0.1 wt%

Spices

Preservative

Purified water level

4. Cream

Seaweed extract 0.5% by weight

Glycerin 3.0 wt%

Stearic acid 8.0 wt%

Squalane 5.0 wt%

Self-emulsifying glycerin monostearate 2.5% by weight

Monostearic acid polyoxyethylene sorbitan 1.5 wt%

Propylene Glycol 4.0 wt%

0.2% by weight of stearyl glycyrrhetinate

Vaseline 2.0 wt%

Antioxidant Proper

Spices

Preservative

Purified water level

Claims (6)

1. Cosmetic composition for whitening or wrinkle improvement containing the seaweed extract as an active ingredient.
2. The cosmetic composition of claim 1, wherein the laver is radiation pattern laver (Porphyra yezoensis).
3. According to claim 1, The laver extract is obtained by extracting, separating and purifying dried laver with water, C ₁ ~ C ₄ alcohol or a mixed solvent of water and C ₁ ~ C ₄ alcohol, whitening or wrinkle improvement Cosmetic composition for.
4. According to claim 3, wherein the C ₁ ~ C ₄ alcohol is characterized in that 1 ~ 100% methanol or 1 ~ 100% ethanol, cosmetic composition for whitening or wrinkle improvement.
5. According to claim 1, Seaweed extract is characterized in that contained in 0.0001 to 30.0% by weight relative to the total weight of the cosmetic composition, cosmetic composition for whitening or wrinkle improvement.
6. The composition of claim 1, wherein the composition is formulated as a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. Or cosmetic composition for improving wrinkles.

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