KR20100019646A - Cosmetic composition using antiperspirants and deodorants having cortex hibiscus extract - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Cortex Hibiscus is provided to suppress 2-nonenal formation without side effects inside the skin. CONSTITUTION: A composition for removing body odor contains 0.0001-20 weight% of Cortex Hibiscus extract as an active ingredient. The Cortex Hibiscus extract is obtained using hydrous or anhydrous low alcohol of C1-C4, ethyl acetate, or acetone. The cosmetic composition is used in the form of lotion, aqueous solution, spray, suspension, emulsion, powder, granule, and gel.

Description

Cosmetic composition for restriction deodorization containing neck root extract {Cosmetic composition using antiperspirants and deodorants having Cortex Hibiscus extract}

The present invention relates to a cosmetic composition containing the extract of the neck root. More specifically, the present invention relates to a cosmetic composition containing the extract of the neck root having excellent efficacy of inhibiting the occurrence of body odor.

Human beings can smell and smell each other's body odor and can live without any objection. However, when too much, it causes objection or displeasure from others. Recently, there is an increasing demand for etiquette products that suppress unpleasant body odors, such as sweat, bad breath and armpit odor. It has been found that the development of body odor affects the lifestyles such as eating, smoking, bathing frequency, etc., other than diseases, physical condition, and genetic constitution.

Volatile aldehydes produced from higher fatty acids or esterified derivatives secreted from living organisms have been found to be one of the main causes of body odor, and also body odors specific to older adults (smell of elderly people) that occur with age. Is also beginning to point out. For example, with increasing age, lipid peroxidation increased and body odor components were analyzed by GC-MS, indicating that most of them were aldehydes, and 2-nonenal was found only in people over 40 years of age (J Invest Dermatol. 2001). Apr; 116 (4): 520-4). In addition, the disaccharide trehalose component prevents the generation of volatile aldehydes produced by the decomposition of alpha-linolenic acid, and clinical experiments confirmed that trehalose component is an excellent body odor suppression component by confirming the reduction of unsaturated aldehydes (Pure Appl Chem, Vol. 74, No. 7, pp. 1263-1269, 2002).

In general, sebaceous glands in human skin are secreted through the pores to the skin surface to prevent evaporation of moisture and maintain the elasticity of the skin. However, when the discharged sebum is in contact with the air on the surface of the skin, microorganisms on the skin multiply and thereby oxidize the sebum, thereby making the body bad. In particular, in older age, fatty acids in sebum are oxidized to lipid peroxidation, and odor is generated. 2-nonenal, which is a kind of volatile aldehyde produced from 9-hexadecenoic acid, which is increased among sebum in the middle-aged age, is senile odor. Is the main cause of

In order to reduce the odor, the body odor component is removed through a bath or air circulation in the room, a method of masking it through a fragrance and a deodorant, and the body odor component is adsorbed together with its precursor to volatilize the body odor component. The prevention method, the production itself from the precursor of the body odor component by the oxidation reaction or the activity of bacteria, and the like. The masking method using the above-mentioned fragrance and deodorant does not suppress the body odor component itself, and may cause undesirable new odor due to the mixture of the body odor and the fragrance component in question. It is difficult to solve the problem, and it prevents the oxidation of sebum in the air by inhibiting microorganism inhibitors, enzyme inhibitors, which can minimize odor by destroying and removing a certain amount of bacteria on the skin used to suppress the production and volatilization of body odor. Chemical components such as antioxidants have a weak deodorant effect, but serious problems such as causing irritation to the skin are emerging, and thus, it is urgent to develop a composition having safety and efficacy.

Accordingly, the present inventors have been conceived to solve the problems as described above, it is an object of the present invention to provide a cosmetic composition that effectively inhibits in the body odor generation step by using a herbal composition that can be safely applied to the skin.

According to the present invention, the cosmetic composition containing the extract of the neck root as an active ingredient effectively removes body odor generating substances that may be produced by sebum (fatty acid), but does not show any side effects in the skin.

In order to achieve the above object, it is characterized by containing the extract of the neck root which is a herbal composition having an effect of inhibiting body odor generation according to the present invention as an active ingredient.

Root bark is a stem or root bark of Hibiscus syriacus L., a shrub of 3 to 6 m in height in the Malvaceae family. The Latin name is Cortex Hibiscus. Neck root skin has been used as a herbal medicine for the treatment of fever, detoxification, dysentery, baekdaeha, hemorrhoids (Chongyang Dictionary). The components of the bark skin are known to include substances such as isoeugenol, 2-phenylethanol, acetylbenzaldehyde, indole, and lutein. In addition, the study using the melanoma cells (B16 mouse melanoma cells) of the extract of the neck bark has been confirmed that the melanin production inhibitory effect can be used as a cosmetic composition [registered patent KR 10-0562369].

According to the present invention, the extract of the neck root which can be purchased as a medicinal herb is first extracted with an organic solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms or ethyl acetate or acetone, and then concentrated under reduced pressure, followed by freeze-drying or spray-drying. To prepare. The extract of the neck roots produced in this manner showed very good body odor suppression effect under artificial body odor generating conditions.

The body odor inhibitor of the present invention is prepared by preparing in a suitable state such as liquid, such as lotions, aqueous solutions, sprays, suspensions and emulsions, solids such as powders, granules and blocks, semisolids such as creams and pastes, and gels. can do.

In the present invention, the amount of the extract having the above-mentioned efficacy may be blended in an amount of 0.0001 to 20% by weight, preferably 0.001 to 10% by weight, for the stability of the formulation. When these concentrations were 0.0001% by weight or less, no obvious effect could be expected. At concentrations of 10% by weight or more, no increase in effect was observed with the increase of the content.

Hereinafter, although this invention is demonstrated in detail based on an Example, it turns out that the technical scope of this invention is not limited only to these Examples.

Example 1 Preparation of Root Bark Extract

Purchasing a dry bark sample, 100 g of the ground pulverized powder was poured into 3 L of a solvent such as water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone, and boiled for 3 hours in a reflux extractor with a cooling capacitor. After filtering with 100 mesh filter cloth, the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and freeze-dried to obtain a crude extract of 1% of dry content. .

Experimental Example 1 Evaluation of the Effect on the Production of 2-nonenal from Palmitoleic Acid (9-hexadecenoic Acid)

The effects of neck root skin extract were added to the 2-nonenal production step, which is the main cause of body odor by heating fatty acids.

As fatty acids, 100 mg of palmitoleic acid, 500 mg of cellulose powder and 1.25 ml of 0.12M phosphate buffer (pH 6.0) were collected in a 20 ml glass vial bottle. The extract obtained in Example 1 is added thereto. 1 g of a natural product having a concentration of 1% was added (test group), and a different vial bottle without a test sample was prepared as a control and plugged. These vials were heat treated at 105 ° C. for 1 hour and allowed to cool to room temperature, and then the vial bottles were kept in a heat block preheated to 80 ° C. After holding for 5 minutes, 2 ml of each head space gas (hereinafter simply referred to as “HSG”) was collected from each vial with a gas syringe.

The collected HSG was analyzed for 2-nonenal content by mass spectrometry (hereinafter simply referred to as "GC / MS"). The GC / MS instrument (Varion CP3800 / Varian 1200L) and the analytical column were CP WAX 52CB 30 m capillary column (25 mm). Using helium gas at a flow rate 1ml / min as a carrier gas, and a sample injection port temperature was 200 ℃ and column hangoncheung temperature is 80 ^o for 5 minutes at C and 200 ^o C and then to the temperature rise per minute 5 ^o C 200 ^o C It was kept for 5 minutes at. As a standard sample, 2-nonenal, which is a volatile aldehyde, was used. The content of 2-nonenal in the HSG was calculated according to the peak area of the mass spectrometer of the standard sample and the HSG sample.

[Table 1] Analysis of body odor component

GC CP-3800 Varian Column CP WAX 52CB 30 m capillary comumn (25 mm) Oven temperature 80 ^o C (5 min) → 200 ^o C (5 min), 5 ^o C min ^-1 Injector temperature 200 ^o C MS 1200L Quadrupole, Varian Ionization voltage 70 eV Carrier gas He (1 mL min ^-1 )

In addition, each sample was subjected to a sensory test on ten samples of the HSG sample from the viewpoint of body odor, particularly in terms of odor. The panelist was directly smelled by each vial, and the intensity of the human body-like odor in the sample to which the test group was applied was compared with the degree of odor of the control system, and was determined in four steps as follows.

· At least about the same (+++)

Slightly weak (++)

Obviously weak (+)

Can't feel smell (-)

Each sample was evaluated by combining the determination results by the entire paneler.

TABLE 2

sample 2-nonenal content in HSG (mg) Sensory evaluation Control 5.63 +++ Neck Root Extract ^- +

As shown in Table 2, the production of 2-nonenal from fatty acids (9-hexadecenoic acid) was 5.63 mg in the control group, but it was confirmed that 2-nonenal was not detected in the treatment group of the neck root extract. As a result of sensory evaluation, it was confirmed that the generation of body odor-like odor was suppressed in the extract of the neck root skin extract.

[Experimental Example 2] Effect of Mokeun Root Extract on Volatilization of 2-nonenal

As a result of adding the extract of the neck root to the 2-nonenal production step, which is the main causative agent of body odor, by heating the fatty acid through Experimental Example 1, it was confirmed that 2-nonenal was not detected. Whether the product itself was inhibited or influenced by volatilization of 2-nonenal by natural products was investigated.

10 mg of 2-nonenal, 100 mg of palmitoleic acid (9-hexadecenoic acid), 500 mg of cellulose powder and 1.25 ml of 0.12 M phosphate buffer (pH 6.0) were collected in a 20 ml glass vial bottle. To this was added a test sample of the neck root extract. 1 g of myofiformis extract with 1% concentration was added (test group), and a different vial bottle without a test sample was prepared as a control and plugged. These vials were kept in a heat block preheated to 80 ° C. After holding for 5 minutes, 2 ml of each head space gas (hereinafter simply referred to as “HSG”) was collected from each vial with a gas syringe. It was measured by GC-MS in accordance with the nonenal content experiment 1 in the collected HSG. The results are shown in Table 4.

[Table 3]

sample 2-nonenal content in HSG (mg) Control 1.110 Neck Root Extract 1.796

As shown in Table 3, 2-nonenal was detected in the HSG in the case of the extract of the neck root as in the control system. From these results, it was confirmed that the extract of the neck root inhibits the production of 2-nonenal but does not inhibit volatilization itself.

[Experimental Example 3] Evaluation of the Effect of Molecular Root Extract on the Production of 2-nonenal from Palmitoleic Acid (9-hexadecenoic Acid) under Human Body Temperature

As fatty acids, 100 mg of palmitoleic acid (9-hexadecenoic acid), 500 mg of cellulose powder, and 1.25 ml of 0.12 M phosphate buffer (pH 6.0) were collected in a 20 ml glass vial bottle. A test sample of myocardium extract was added. 1 g of the myocardium extract having a concentration of 1% was added (test group), and a different vial bottle without a test sample was prepared as a control and plugged. Two sets each of the same composition were prepared together with the test group and the control group. These vials were left to stand in the constant temperature layer of 37 degreeC under the illuminance condition of 9000 lux by fluorescent lamp. One of the vial bottles containing two sets of the same composition was kept in the heat block preheated at 80 ° C. on the first day of standing and the other on the seventh day of standing. After holding for 5 minutes, 2 ml of each head space gas (hereinafter simply referred to as “HSG”) was collected from each vial with a gas syringe. The nonenal content in the collected HSG was measured by GC / MS according to Experiment 1. And the smell from the vial bottle after HSG collection was evaluated by the sensory test according to Experiment 1.

TABLE 4

sample 2-nonenal content in HSG (mg) Sensory evaluation Neglect Day 1 Control - + Neck Root Extract - + 7 days left Control 5.13 +++ Neck Root Extract 0.2 +

As shown in Table 4, the production of 2-nonenal from 9-hexadecenoic acid at 37 ° C. was not significantly different from the control group of the extract of the neck roots on day 1, compared to the control, whereas on day 7, In comparison, the production of 2-nonenal was significantly inhibited in the extracts treated with the myocardium extract. From this, it was confirmed that the extract of the neck root was effective in suppressing the generation by remarkably inhibiting the production of body odor components from the surface of the human body.

Experimental Example 4 Evaluation of Primary Skin Irritation Test and Cumulative Irritation Test of Root Bark Extract

In order to evaluate the safety of the cosmetic composition containing the extract of the neck roots on the human body was subjected to the primary stimulation test and cumulative stimulation test through the human patch test (Human patch test) as follows.

Twenty healthy men and women were tested according to CTFA guidelines (Ref .: The Cosmetic, Toility and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 20 μl of the sample was dropped into a Fin chamber, and it was placed on the inside of the arm of the human body, which was the test site, and fixed with tape. In the first skin irritation test, the patch was removed for 24 hours, the patch was removed, and after 4 hours and 24 hours, the skin reaction of the test site was determined according to the following criteria, and the results are shown in Table 5. The cumulative skin irritation test was carried out by the repeated insult patch test (RIPT) method, and 9 samples were repeatedly treated as an induction phase. After 10 days, the degree of irritation was evaluated visually after the sample was treated as a challenge phase. Shown in

Criteria

-; No erythema or unusual symptoms

+-; Slightly redder than the surroundings

+; Significantly redder than the surroundings

++; Severe redness and swelling than around

(formula)

Stimulus = [{(±) number x 1} + {(+) number x 2} + {(++) number x 3}] / number of subjects

TABLE 5 Primary skin irritation test

sample Number of subjects Judgment result Stimulus ++ + +- - Control group (distilled water) 20 - - - 20 0 Neck Root Extract 20 - - - 20 0

[Table 6] Cumulative skin irritation test

sample Number of subjects  Induction phase challenge phase Stimulus One 2 3 4 5 6 7 8 9 24hr 48hr 72hr Control group (distilled water) 20 - - - - - - - - - - - - 0 Neck Root Extract 20 - - - - - - - - - - - - 0

As a result of evaluating the skin irritation test on the human body to determine whether the application of the extract of the neck roots as a cosmetic material, it was confirmed that it is a safe material to be applied as a skin irritation relief material.

The cosmetic composition containing the extract of the neck root of the present invention can be used in a variety of cosmetics, etc., the formulation can be selected arbitrarily. Products to which the composition may be added include body cleansers, skin cleansers, and body lotions, external skin preparations.

The following shows a specific formulation example of the cosmetic composition having the effect of inhibiting the body odor generation of the present invention, the formulation comprising the cosmetic composition of the present invention is not limited thereto.

Formulation Example 1 Body Cleanser

division weight % Sodium Laureth Sulfate 30 N-lauryl-L-glutamic acid triethanolamine 30 Cocamidopropyl Bane 10 KCl 1.5 Concentrated glycerin 3.000 Polyquaternium 0.5 Neck Root Extract 5 Incense / Preservative Quantity Purified water to 100

Formulation Example 2 Body Lotion

division weight % Cetostearyl alcohol  2.5 Behenyl Alcohol  1.0 Squalene 5.0 Beads wax  2.0 1,3-butylene glycol  5.0 Concentrated glycerin  5.0 Cabopol 0.1 Triethanolamine 0.1 Cyclopentasiloxane  4.0 Neck Root Extract 5.0 Incense / Preservative Quantity Purified water to 100

Claims (3)

Restrictive deodorant cosmetic composition containing neck root extract as an active ingredient Cosmetic composition wherein the composition inhibits the production of 2-nonenal The cosmetic composition according to claim 1 or 2, wherein the content of the composition is 0.0001 to 20% by weight.