KR20100001376A - New method for preparing the functional fermented organic sulfur - Google Patents

Abstract

PURPOSE: A method for preparing functional fermented organic sulfur is provided to obtain nontoxic functional sulfur by aging sulfur in a herbal medicine extract, roasting it using needle-leaf trees, and then fermenting the herbal medicine extract with yeast. CONSTITUTION: A method for preparing functional fermented organic sulfur comprises the steps of: pulverizing sulfur in a 10~50 mesh size; adding 15~20 times weight of water to a herb medicine mixture consisting of glycyrrhizae radix 40~60 weight%, pine needles 30~40 weight%, and Zingiberis Rhizoma crudus 20~30 weight%, and then performing hot water extraction at 100°C for 4~10 hours; mixing pulverized sulfur 60~90 weight% and herbal medicine hydrothermal extract 10~40 weight% and then aging the mixture at a room temperature over 2 hours; inserting the aged sulfur into the holes of needle-leaf trees, sealing with loess, and then roasting it at a high temperature of 600°C or more; repeating a herbal aging process and a coniferous tree roasting process over 9 times; mixing the collected sulfur with red ginseng 30~40 weight%, laminaria japonica 30~40 weight%, rice bran 20~30 weight%, garlic 20~30 weight%, and bamboo leaf 10~20 weight%, and then adding 15~25 times water to a herbal medicine mixture at 90~100°C for 4-10 hours; inoculating the hydrothermal extract to a Sacchanomyces Cerevisiae culture medium and fermenting it at 20~35°C for 24~72 hours; and radiating 20~40KHz(300W) ultrasonic waves and 2450MHz(1KW) microwaves.

Description

New Method for preparing the functional fermented organic sulfur

According to the present invention, sulfur is used as a main ingredient to ripen oriental medicine using useful medicines and roasted by conifers such as pine, and then repeat the above-described herbal ripening and coniferous roasting process at least nine times. And a method for producing nano functionalized organic fermented sulfur by dispersing with microwave.

Sulfur is a yellowish non-metallic solid in oxygen that is highly thermotoxic and can only be detoxified. It is a component of sulfur compounds, such as methionine and cysteine, used to repair and treat somatic tissues such as skin, blood vessels, organs and joints. . In particular, sulfur has various effects such as detoxification, anti-cancer, anti-inflammatory, increased blood flow, skin disease treatment, anti-aging, lowering cholesterol, and thrombolysis, and synonymous feelings include efficacy of anti-cancer, anti-inflammatory, pain relief, and skin disease treatment. It is used as health food, medicine, agricultural supplies, building supplies, feed additives, and bath beauty supplies.

As the legislation and detoxification method of sulfur, it is known that heat treatment methods such as melting and roasting by mixing with oriental medicine and the use of animals and plants such as sulfur duck and sulfur garlic are known, but the development of new legislation method using fermentation is required. It's happening.

The present invention is to solve the above required situation, and to select and contain a detoxifying herbal medicine with sulfur, and to puncture herbal pine trees and the like for the ripening and harmful toxicity substitution by sap components of conifers such as pine Built-in roasted at high temperature, the process was repeated nine times or more, and then fermented with herbal extracts and yeast to produce organic sulfur that was removed by the organic process of sulfur by the fermentation process of yeast. Dispersed by the method to prepare a sulfurized nano-sulfur solution, and then concentrated freeze-drying to produce a non-toxic sulfur with improved pharmacological, functional.

Herbal ripening step (first step) of aging by mixing licorice, pine needles, ginger extract with sulfur to achieve the above object;

Coniferous roasting step (second step) to roast the high-temperature 600 ° C or more after sealing the mature aged sulfur of the first step in the conifers such as pine and then sealed with high loess high far-infrared emissivity;

A repeating step (third step) of repeatedly performing the first step and the second step nine times;

A yeast fermentation step (fourth step) of fermenting the soybean matured coniferous roasted sulfur of the third step with red ginseng, kelp, rice bran, garlic, bamboo leaf extract and yeast (Saccharomyces Cerevisiae);

The yeast fermented sulfur of the fourth process is treated by dispersing (emulsion) by ultrasonic wave and microwave at the same time to produce a nano-aged herbal fermented sulfur aqueous solution to extract concentrated step of filtration and lyophilization (the fifth step) functional fermentation organic Nontoxic sulfur can be produced.

Functional fermented organic sulfur according to the present invention can provide detoxified sulfur by aging by oriental medicine with detoxification function and repetitive detoxification action by coniferous roasting step, and extracting detoxified inorganic sulfur from herbal extract and yeast By fermentation using the yeast fermentation process can provide the organic sulfur by using the yeast metabolism function. Furthermore, by dispersing effect by the parallel treatment of ultrasonic wave and microwave, herbal fermented organic sulfur and yeast are decomposed to provide nano-toxic non-toxic organic sulfur.

The configuration in the present invention will be described in detail as follows.

1. Herbal ripening step of sulfur (1st process)

The sulfur is sorted and coarsely pulverized into 10-50 meshes. And licorice 40 to 60% by weight, pine needles 30 to 40% by weight, ginger 20 to 30% by weight, and purified water at 15 to 25 times the weight ratio to the herbal mixture by adding hot water at 90 to 100 ° C for 4 to 10 hours To prepare the extracted herbal medicine hot water extract.

60 to 90% by weight of the crude pulverized sulfur by mixing the herbal medicine hot water extract 10 to 40% by weight at room temperature for 2 hours or more.

2. Coniferous roasting step (2nd process)

Chinese herbal matured sulfur prepared in the first step is made by drilling a hole in conifers such as pine and then sealed with ocher and then roasted at a high temperature of 600 ° C or more.

At this time, the type of conifer used is characterized in that it is used to select one of pine, pine, fir, bark, yew.

3. Repeat execution step (3rd process)

The first step and the second step are repeated nine times or more.

In other words, when the first step and the second step is completed, the herbal matured sulfur mass is recovered, and then the medicinal herb maturing process according to the first process and the coniferous roasting process according to the second process are repeatedly performed nine times or more.

4. Yeast Fermentation Stage (Step 4)

Recovered sulfur repeatedly carried out 9 times or more by the third process and separately 30-40% by weight of red ginseng, 30-40% by weight of kelp, 20-30% by weight of rice bran, 20-30% by weight of garlic, 10-20 weight of bamboo leaves 15% to 25 times the amount of purified water was mixed and diluted to a concentration of 1 to 30% by weight in the herbal extract extracted hydrothermally for 4 to 10 hours at 90 ~ 100 ° C 5 ~ 15% by weight of the yeast culture medium Inoculate and shake fermentation at 20 ~ 35 ° C for 24 ~ 72 hours.

At this time, yeast is Sacchanomyces Cerevisiae culture medium is PDB liquid medium.

5. Dispersion and concentration step (5th process)

Yeast fermented sulfuric acid aqueous solution according to the fourth step at a temperature of 90 ~ 100 ° C in parallel treatment of 20 ~ 40 kHz ultrasonic wave and 2450MHz microwave to disperse for 4 to 24 hours to prepare a nano-organic sulfuric acid aqueous solution and then filtered Concentrated lyophilization produces functional fermented organic sulfur.

Hereinafter, the content of the present invention will be described in detail through examples.

However, this embodiment is only an example of the present invention and does not limit the rights of the present invention.

Example

1) Crude 1 kg of sulfur into 30 mesh.

2) 50% by weight of licorice, 30% by weight of pine needles, 20% by weight of ginger mixed in a mixed ratio to the total weight, and then added 1:20 times the purified water in a weight ratio to the herbal mixture by extracting hot water at 100 ° C for 4 hours. do.

3) 70% by weight of sulfur of 1) is mixed with 30% by weight of the herbal medicine hot water extract of 2) is aged at room temperature for 2 hours.

4) After the hole of 10cm in diameter is made in the green pine, 15cm in diameter, and then the oriental maturated sulfur of 3) is embedded, and both sides are sealed with ocher.

5) The dried pine should be roasted the pine containing 4) sulfur matured sulfur. The roasting temperature should be at least 600 ° C (sulfur melting point above 411 ° C).

6) After the end of step 5), only the sulfur mass is recovered and aged by step 3) as an oriental medicine hot water extract solution according to step 2) and the pine lining and sealing by step 4) and roasting by step 5). Repeat the process nine times or more.

7) 30% by weight of red ginseng, 30% by weight of kelp, 20% by weight of rice bran, 10% by weight of garlic, 10% by weight of bamboo leaves in a mixed ratio to the total weight and then added 1:20 times the purified water to the herbal mixture by weight By adding hot water extraction at 100 ° C. for 4 hours to prepare a herbal extract.

8) After adding the herbal extract of 7) to the sulfur repeated 9 times or more, and diluting to 5% by weight of sulfur, the yeast culture (Sacchanomyces Cerevisiae) cultured in PDB medium was Inoculate 10% and ferment for 24 hours at 25 ° C.

9) The shake fermented yeast fermented sulfur solution was heated to 90 ° C. to kill yeast, and 28 kHz (300 W) ultrasonic wave and 2450 MHz (1 ㎾) microwave were treated for 4 hours to disperse, followed by sulfur solution. Filtration concentrated to prepare lyophilized functional fermented organic sulfur.

Experimental Example 1) Functional fermentation of organic sulfur according to an embodiment of the present invention was tested for NO (Nitricoxide) product by Raw 264.7 macrophages of organic sulfur to measure the immune activity.

Table 1 NO Production Amount by Samples of Examples

Conc uM Of NO Reference 0.5mg / ml 32.83 ± 0.12 row264.7 (+ LPS) 1mg / ml 37.56 ± 0.73 row264.7 (+ LPS) 2mg / ml 40.11 ± 0.05 row264.7 (+ LPS)

Experimental Example 2) Functional fermentation of organic sulfur according to an embodiment of the present invention was carried out by MTT assay of HT1090 Human Cancer Cell to measure cancer cell viability.

<Table 2> MTT assay by the sample of Example

Conc Cell Viability (%) Reference 0.5mg / ml 15.24 ± 1.72 HT1080 / MTT 1mg / ml 17.89 ± 1.45 HT1080 / MTT 2mg / ml 20.32 ± 2.24 HT1080 / MTT

As shown in Table 1 and Table 2, the functional fermented organic sulfur according to the present invention was confirmed to have an immune and anticancer activity.

1 is a process flow diagram schematically showing a method for producing functional fermented organic sulfur according to the present invention.

Claims (2)

In the production method of functional fermented organic sulfur Sulfur is coarsely pulverized with 10 to 50 mesh, mixed with 40 to 60% by weight of licorice, 30 to 40% by weight of pine needles and 20 to 30% by weight of ginger, in a ratio of the total weight to the total weight and 15 to 20 times Purified water was added at a weight ratio of 4 to 10 hours to prepare an extract extracted from hot water at 100 ° C, and then 60 to 90% by weight of the crude pulverized sulfur and 10 to 40% by weight of the herbal medicine hot water extract were mixed at room temperature for 2 hours or more. Aging step (first step); The herbal matured sulfur prepared in the first step is made by drilling a hole in conifers such as pine and then sealing it with ocher and then roasting at a high temperature of 600 ° C. or higher (second step); A repeating step (third step) of repeatedly performing at least nine times the herbal ripening step of the first step and the conifer roasting step of the second step; The sulfur repeatedly repeated by the third step was recovered and the total weight of red ginseng 30-40% by weight, kelp 30-40% by weight, rice bran 20-30% by weight, garlic 20-30% by weight, bamboo leaf 10-20% by weight. Mix by mixing ratio, and add purified water to 15-25 times of the medicinal herb mixture and dilute to 1-30 wt% sulfur concentration in the herbal extract extracted hydrothermally for 4-10 hours at 90 ~ 100 ° C in PDB medium Inoculating 5 ~ 15% by weight of the cultured yeast (Sacchanomyces Cerevisiae) culture medium in a sulfuric acid dilution solution and the fermentation shaking at 20 ~ 35 ° C for 24 to 72 hours (fourth step); The yeast fermented sulfuric acid aqueous solution according to the fourth process is 20 to 40 kHz (300 W) ultrasonic wave and 2450 MHz (1 ㎾) microwave at the temperature of 90 ~ 100 ° C in parallel dispersing for 4 to 24 hours to organic Sulfur aqueous solution or method for producing functional fermented organic sulfur, which is filtered and concentrated to freeze-drying (step 5) The method of producing functional fermented organic sulfur according to claim 1, wherein any one selected from pine, pine, fir, cypress and yew is used as the kind of conifer to be roasted.

Images