KR20090111719A - Cosmetic Compositions for whitening Skin containing jujube culture - Google Patents

Abstract

PURPOSE: A cosmetic composition for skin whitening with jujube fermentation liquid is provided to enhance penetration to the skin and maximize the effect of active ingredient. CONSTITUTION: A cosmetic composition for skin whitening contains 0.0001-20.0 weight% of jujube fermentation liquid. Jujube extract is isolated through hot water extraction. The cosmetic composition is used in the form of lotion, the moisturizing cream, the massage cream, the essence, the eye cream, the cleansing cream, the cleansing foam, the cleansing water, the pack, and the spray or the powder.

Description

Cosmetic composition containing jujube fermentation broth {Cosmetic Compositions for whitening Skin containing jujube culture}

The present invention relates to a whitening cosmetic composition containing a jujube fermentation broth, and more particularly, to a skin whitening cosmetic composition containing a fermentation broth obtained by complex fermentation with fermentation microorganisms of lactic acid bacteria and yeast after hot water extraction of jujube.

The cosmetic composition of the present invention has an excellent whitening effect by inhibiting the movement of melanocytes from melanocytes (Melanocyte) to keratinocytes (Keratinocyte), and the active ingredient of the polymer structure is decomposed into a low molecular structure through fermentation to infiltrate the skin. Greatly increases the effect of the active ingredient. In addition, it can be used in the form of a lotion, essences, lotions, creams, packs, gels, ointments, patches, or sprays as a safe substance with no skin irritation.

Human skin color is largely determined by the amount of melanin, hemoglobin, carotene and the like, of which melanin plays the most important role. Melanin not only determines the skin color of humans but also acts as a skin protective action (ultraviolet absorption, free radical scavenger), but excessive skin exposure due to excessive exposure to ultraviolet rays, air pollution and stress. When it is produced inside, pigmentation phenomenon occurs and the skin color becomes black or causes freckles and freckles.

 The melanin synthesis process is as follows. First, an enzyme called tyrosinase generates dopaquinone by using tyrosine in a cell as a substrate, and a melanin copolymer is produced through autooxidation and enzymatic reaction from dopaquinone. The melanin produced in this way is transferred to melanocytes' dendrites through a pocket called melanosomes, which are then delivered to keratinocytes. The mechanism of melanocytes migration to keratinocytes has not been known to date, but the strength of evidence suggests that melanocytes secreted from melanocytes in skin tissue may be involved in the phagocytic function of keratinocytes. It is known that the probability of delivery is very high. This can also be found in the patent [JP, 2002-508331] of the composition content using the pagocytosis inhibitory ability of the soybean trypsin inhibitor (STI) filed by Johnson & Johnson. Melanosomes are also known to be delivered to keratinocytes by the Melanosome Transport Complex (MTC), which is involved in the transfer of melanosomes to melanocyte dendrites. MTC is composed of three proteins, Melanophilin, Rab27a and Myosin 5a, and Rab27a is a protein that binds to the surface of the melanosome and acts as a carrier to recognize and move the melanosomes. In order to meet the myosin 5a protein, which is the intracellular cytoskeleton, it is a protein called melanophilin that acts as an adapter that recognizes and connects the two proteins in the middle. When these three constructs meet to form a complex, it is possible to move the melanosomes to the cell ends normally and ultimately to the keratinocytes. If any one of these three constructs is abnormal, there is a problem in the movement of melanosomes without any problem in the production of melanin, and eventually the skin color becomes brighter. Therefore, the whitening effect can be expected by inhibiting the movement of the melanosomes through the control of this construct.

Therefore, the present inventors have extensively researched and examined in order to develop a substance that effectively inhibits the process of delivering melanocytes to the keratinocytes in the skin, jujube fermentation broth from melanocytes to melanocytes The present invention has been completed by finding that the movement of is effectively suppressed to exhibit an excellent whitening effect. Moreover, since the active ingredient of the polymer structure is decomposed into a low molecular structure through fermentation, the skin penetration effect is greatly increased, thereby maximizing the effect of the active ingredient.

Accordingly, an object of the present invention is to provide a skin whitening cosmetic composition containing the jujube fermentation broth obtained by extracting the jujube extract and fermented with fermentation microorganisms such as lactic acid bacteria and yeast as an active ingredient.

In order to achieve the above object of the present invention, the present invention provides a whitening cosmetic composition containing a jujube fermentation broth fermented using a microorganism in jujube extract.

In the composition, the jujube fermentation broth is characterized in that it contains 0.0001-20.0% by weight relative to the total weight of the composition.

In the composition, the microorganism is characterized in that the fermentation by inoculating at least one selected from the group consisting of lactic acid bacteria, Bacillus subtilis, yeast.

In the composition, the jujube extract is characterized in that it is extracted by the hot water extraction method.

In the composition, wherein the cosmetic composition has a formulation selected from the group consisting of flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder It features.

The present invention can obtain the following effects by the configuration and experimental examples to be described below.

The cosmetic composition of the present invention has an excellent whitening effect by inhibiting the transfer of melanocytes from melanocytes to keratinocytes. In addition, the jujube extract of the present invention, since the active ingredient of the polymer structure is decomposed into a low molecular structure through fermentation, the skin penetration effect is greatly increased, thereby maximizing the effect of the active ingredient to maximize the skin whitening effect.

In addition, the cosmetic composition of the present invention may be used as a safe substance having no skin irritation, in the form of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

Hereinafter, the present invention will be described in more detail.

Jujube used in the present invention refers to the fruit of the date palm (Zizyphus jujube), also known as crude (棗) or wood wheat (木 蜜). The surface is reddish brown, elliptical, 1.5 ~ 2.5cm long, and ripe when red. Jujube fruit has 20-49% of dry matter, and 14-42% is composed of sugars such as fructose, sucrose, glucose and rhamnose. In addition, organic acids such as malic acid, grape acid, succinic acid and glycolic acid are contained 0.54 to 3.4% and are rich in minerals such as potassium, calcium and magnesium. Fruits are not only reproduced, but also processed in life and used as jujube, jujube tea, jujube vinegar and jujube. In oriental medicine, it is used as a diuretic, tonic, and emollient. In addition, in oriental medicine, the muscles are aching disease, when the muscles are painful, cough, when the whole body hurts are mainly used and is known to be effective in exfoliating skin with anti-allergic effect.

Jujube extract provided in the present invention can be obtained using a variety of extraction methods known in the art. Thereafter, for the fermentation process by fermentation microorganisms, hot water extraction using water is most preferred.

In the present specification, the term "fermentation" refers to a process in which microorganisms decompose organic materials using enzymes they have. Fermentation and decay reactions proceed by a similar process, but when decomposition results in a substance that is useful in our lives, it is called fermentation. Most organisms need oxygen for breathing, and oxygen breathing gives them the energy they need to work. On the other hand, creatures living in an oxygen-deficient environment, such as deep in the ground or the bottom of a lake, can adapt to the environment and get energy without oxygen. This breathing is called anoxic breathing. Fermentation is one such oxygen-free breath. When a person consumes organic matter such as carbohydrates, oxygen breaks down the organic matter into carbon dioxide and water and gains energy. However, anaerobic breathing organisms do not completely decompose organic matter, but produce other kinds of organic matter. In addition, the amount of energy generated is small. During fermentation, too, organic matter is decomposed, producing another organic matter and generating a small amount of energy. For example, put yeast in a bowl of glucose and close the lid. Yeast, a microorganism, uses enzymes to break down organic glucose. At this time, because the lid is covered, breathing through oxygen is impossible and oxygen-free breathing takes place. Yeast does not completely break down glucose, making ethanol. This fermentation is called alcohol fermentation, and alcohol fermentation can be used to make liquor such as makgeolli or beer. In addition, when Lactobacillus reacts with glucose in the anaerobic state, lactic acid is produced. Such fermentation is called lactic acid fermentation. Yogurt, cheese, and kimchi are produced by lactic acid fermentation.

Through such fermentation, the substance is naturally decomposed by microorganisms and enzymes secreted by microorganisms, so it becomes smaller than 80 nm on average, which facilitates skin absorption. You have an advantage. In addition, toxic substances such as heavy metals, residual pesticides and the like harmful to the human body are completely decomposed by the microorganisms and are harmless to the human body.

The microorganisms used in the fermentation of the present invention are not particularly limited, and preferably include lactic acid bacteria (Lactobacillus genus, Streptococcus genus, etc.), Bacillus subtilis (Bacillus genus), yeast ( Saccharomyces genus), fermentation by the microorganisms Is about 24 to 120 hours at 25 to 50 ° C, preferably 48 to 72 hours at 30 to 40 ° C.

The cosmetic composition of the present invention preferably comprises 0.001 to 20.0% by weight of the jujube fermentation broth based on the total cosmetic composition. When the cosmetic composition of the present invention contains the jujube fermentation broth at less than 0.001 weight, it is difficult to expect the actual whitening effect of the cosmetic composition, and when added in excess of 20.0% by weight, it affects the stability of the formulation and the product. It is not economical because the synergy effect from input is small. More preferably, the cosmetic composition of the present invention comprises 0.01 to 10.0% by weight of the extract, even more preferably 0.1 to 5.0% by weight.

Therefore, the cosmetic composition containing the jujube fermentation solution of the present invention has an excellent whitening effect by inhibiting the migration of melanocytes from the melanocytes to the keratinocytes by the jujube fermentation solution. Moreover, the jujube extract of the present invention can be decomposed into a low molecular structure of the active ingredient of the polymer structure through fermentation, thereby greatly increasing the skin penetration effect, thereby maximizing the skin whitening effect.

The cosmetic composition containing the jujube fermentation broth contained as an active ingredient in the composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, softening water, astringent makeup, nutrient cosmetics, eye cream , Nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, packs and the like is not particularly limited and may be prepared according to the formulation or use of cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

[ Production Example  1: Preparation of Jujube Extract]

Firstly, 50 g of the dry weight of jujube was added in a volume of 10-20 times water as an extraction solvent. It was heated at 70 ° C. for about 1 hour. Subsequently, the mixture was cooled to room temperature and then aged at low temperature at 4 ° C. for 7 days to obtain jujube extract by hot water extraction.

[ Production Example  2: Preparation of Jujube Fermentation Broth]

200 mL of jujube extract prepared in Preparation Example 1 was placed in a culture vessel, and lactobacillus ( Lactobacillus genus) and yeast ( Saccharomyces genus) were inoculated at 0.01% by weight of jujube extract, respectively, and fermented for 72 hours in a shaking incubator under 35 ° C. It was used after removing microorganisms of lactic acid bacteria and yeast from jujube fermentation broth through microfiltration.

[ Experimental Example  1: jujube fermentation broth Melanosome  Movement inhibition effect]

Keratinocytes and melanocytes were isolated from the foreskin of newborns, respectively. The epidermis was isolated from the foreskin tissue by treatment with 0.25% trypsin, followed by 7.5 mg / ml bovine pituitary extract, 0.1 mg / ml human epidermal growth factor (hEGF), 0.5 mg / ml hydrocortisone, 50 mg / ml Cells were separated by stirring with KGM (Clonetics) medium containing gentamicine, 50 mg / ml amphotericin and 5 mg / ml insulin, and cultured in T75 flasks to prepare keratinocytes and melanocytes. Respectively obtained. At this time, the cultured melanocytes were stained with carboxy fluorescein diacetate (CFDA) to analyze the melanosomes. For experiments, keratinocytes and melanocytes were co-cultured in coculture medium (KGM + (KGM-TPA-hydrocortisone)). Then, jujube extract and jujube fermentation broth were respectively added 5.0 mmol / L to some of the cells co-cultured at 12 hour intervals for 6 days. It was then reacted with a mouse monoclonal antibody against anticytokeratin (Zymed Laboratories, United States) as a primary antibody to separate keratinocytes by fluorescence activated cell sorting (FACS), and then combined with phycoerythrin as a secondary antibody. Anti mouse IgG (Burlingame, United States) and the cultured cells were reacted for 30 minutes at 37 ℃. The cultured cells were then removed from the flasks and analyzed using EPICSXL flow cytometer (Coulter Cytometry, Coulter Corp., USA). In FACS analysis, keratinocytes in response to phycoerythrin at 575 nm were analyzed, and melanosomes of keratinocytes were confirmed by measuring CFDA at 525 nm. The results obtained in the coculture were analyzed using Coulter XL software (Coulter Corp.). Melanosome migration inhibition rate was calculated by the following equation 1, the results are shown in Table 1 below.

Melanosome migration inhibition rate (%) = 100-{(fluorescence of sample / fluorescence of control) * 100}

Neon % Inhibition of migration Keratinocyte alone 0.225 - Coculture of keratinocytes + melanocytes 20.2 0 Coculture of keratinocytes + melanocytes with jujube extract 17.7 12.5 Coculture of keratinocytes + melanocytes with jujube fermentation broth 15.3 24.5

As can be seen from the results of Table 1, it was confirmed that the melanoma migration inhibition rate was highest in the jujube fermentation broth.

[ Production Example  3: Example  And Comparative example]

The production method of Examples and Comparative Examples 1 and 2 is as follows.

1) Warm the raw material 1-6 to 70 ° C while stirring to make an oily solution.

2) Add raw material 7-8 and raw material 10-11 to purified water, and warm to 70 ° C while stirring to form an aqueous solution.

3) 1), which is an oily solution, and the raw material 9 are mixed in 2), followed by emulsification and cooling.

Example 3 is obtained by adding a jujube fermentation solution of the raw material 13 and the fragrance of the raw material 15, the preservative added to 3) and cooled to 30 ℃, Comparative Example 1 is a jujube extract of the raw material 14 and the raw material 15 in 3), It is obtained by adding and mixing a preservative and cooling it to 30 ° C., and Comparative Example 2 is obtained by adding and mixing the fragrance and preservative of raw material 15 to 3) above and cooling it to 30 ° C.

ingredient Content (% by weight) Example Comparative Example 1 Comparative Example 2 1. Vaseline 7.0 7.0 7.0 2. Liquid paraffin 10.0 10.0 10.0 3. Beeswax 2.0 2.0 2.0 4. Polysorbate-60 2.0 2.0 2.0 5.Solbitan Sesquilate 2.5 2.5 2.5 6. Squalane 3.0 3.0 3.0 7.propylene glycol 6.0 6.0 6.0 8.Glycerin 4.0 4.0 4.0 9.triethanolamine 0.5 0.5 0.5 10. Carboxyvinyl Polymer 0.5 0.5 0.5 11.Tocopherol Acetate 0.1 0.1 0.1 12.purified water To 100 To 100 To 100 13.jujube fermentation broth 4.0 - - 14.jujube extract - 4.0 - 15.Fragrance, Preservative a very small amount a very small amount a very small amount

[ Experimental Example  2: skin whitening effect test]

In this experiment, the skin whitening effect was tested on the experimenter with healthy skin in Example containing jujube fermentation broth and Comparative Example 1 containing jujube extract, and Comparative Example 2 not containing both jujube fermentation broth and sample of jujube extract. .

20 women aged 20 to 30 were divided into 10 groups A and 10 groups B, respectively, and the cosmetics of Example 2 were applied to the right side of the group A, and the cosmetics of Comparative Example 2 to the left side of the face. The cosmetics according to Comparative Example 1 were applied to the right side, and the cosmetics according to Comparative Example 2 were applied to the left side of the face twice a day for two consecutive months. After completion of the test, the skins of the application areas on both sides of the face were compared by using an image analyzer and a color difference meter, and the darkest color was set to 5, the middle color to 3, and the brightest color to 1, and the intermediate level was estimated. The results of the skin whitening effect are shown in Table 3.

A group B group Experiment number Example Comparative Example 2 Comparative Example 1 Comparative Example 2 Right skin color Left skin color Right skin color Left skin color One 2.5 4 3 4 2 2 3 4.0 4.5 3 3 3 4 4 4 3 5 2.5 3 5 2 2.5 2 2 6 2 4 3 3.5 7 2 2.5 2 2 8 1.5 2 2 2.5 9 2.5 3.5 2.5 3 10 One 2.5 3.0 3.5

As shown in Table 3, it was found that the whitening effect was excellent in the right facial skin of the group A tester who applied the cosmetic according to the embodiment containing the jujube fermentation broth.

From this experiment, it was found that the cosmetics containing the jujube fermentation broth were superior in the whitening effect than the cosmetics containing the jujube extract and the control.

[ Experimental Example  3: skin Patch  exam ( Patch Test )]

Skin patch experiment was conducted to investigate the skin irritation of the jujube fermentation broth of the present invention. Twenty subjects (mean age 29 years, age distribution 23-39 years) were subjected to skin patch experiments on the upper arm using the examples of Preparation Example 3 and Comparative Examples 1 and 2 above. However, psoriasis, eczema, and other skin lesion holders, pregnant women, lactating or contraceptives, antihistamines, etc. were excluded from the experiment.

First, the test area was wiped with 70% ethanol, dried, and the prepared test material was dropped in 15 ㎍ by Finn chamber (Finn chamber, 100x10, EPITEST, Finland) and then airtightly wrapped in the inner part of the subject's forearm. The patch was marked for 24 hours, the patch was removed, and the test site was marked with a pen. After 1 hour and 24 hours after the marking, the magnification (8MC-150, DAZOR, United States of America) was used to observe the test site, and erythema and edema were observed.

The skin response was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG), and the mean skin response rate was calculated according to Equation 2. The results are shown in Tables 4 and 5 below.

* Evaluation criteria and score of skin reaction sign score Evaluation standard - 0 No response ± 0.5 Faint or light erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and vesicles +++ 3 Severe crust and alveolar, scleroderma

Mean skin responsiveness (mean score) = (score × number of responses × 100 × 1/2) / [3 (maximum score) × total number of subjects (n)]

Test substance 1 hour later 24 hours later % Reactivity (n = 20) ± + ++ ± + ++ Example 2 - - - - - 0.83 Comparative Example 1 2 - - - - - 0.83 Comparative Example 2 2 - - - - - 0.83

As can be seen from Table 5, it was found that the cosmetic composition (Example) containing the jujube fermentation broth of the present invention has almost no skin irritation.

[ Experimental Example  4: stability test of the formulation]

The stability test for the cosmetic containing the jujube fermentation broth of the present invention was carried out by the following method.

In order to test the stability of the cosmetics, Examples and Comparative Examples 1 and 2 were kept in an opaque glass container in a constant temperature bath at a constant temperature of 45 ° C. and stored for 12 weeks in a completely shaded refrigerator kept at a constant temperature of 4 ° C. After storing in an opaque glass container for 12 weeks, the discoloration, odor, and degree of separation were measured. The results are summarized in Table 5 below. At this time, the degree of product discoloration, odor and separation were evaluated by classifying into the following six grades: 0: no change; 1: very little separation (discoloration); 2: a little separation (discoloration); 3: slightly severe separation (discoloration); 4: severe separation (discoloration); And 5: extremely severe separation (discoloration). The results are shown in Table 6 below.

division Formulation Stability Example Comparative Example 1 Comparative Example 2 Thermostat Refrigerator Thermostat Refrigerator Thermostat Refrigerator 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0 0 0 0 0 0 Deodorant 0 0 0 0 0 0 detach 0 0 0 0 0 0

As can be seen in Table 6, Examples and Comparative Examples 1 and 2 were stable at 45 ° C. with little discoloration, discoloration or separation, and the examples containing the jujube fermentation broth were present in a stable state in the formulation. .

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition containing the jujube fermentation broth of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

[ Formulation example  1: Softener (Skin Lotion)]

As shown in the following table, the softening lotion was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Jujube fermentation broth 1.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

[ Formulation example  2. Nutritional Cosmetics ( Milk Croissant )]

Nutrients were prepared according to a conventional method as shown in the table below.

Compounding ingredients Content (% by weight) Jujube fermentation broth 2.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

[ Formulation example  3. Nutrition Cream]

Nutritious cream was prepared according to a conventional method as shown in the following table.

Compounding ingredients Content (% by weight) Jujube fermentation broth 4.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

[ Formulation example  4. Massage cream]

Massage cream was prepared according to a conventional method as shown in the table below.

Compounding ingredients Content (% by weight) Jujube fermentation broth 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

[ Formulation example  5. Pack]

Packs were prepared according to conventional methods as shown in the table below.

Compounding ingredients Content (% by weight) Jujube fermentation broth 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (5)

A whitening cosmetic composition containing a jujube fermentation broth fermented by using microorganisms in jujube extract. The cosmetic composition according to claim 1, wherein the jujube fermentation broth contains 0.0001-20.0% by weight based on the total weight of the composition. According to claim 1, wherein the microorganism is a cosmetic composition, characterized in that the fermentation by inoculating at least one selected from the group consisting of lactic acid bacteria, Bacillus subtilis, yeast. The cosmetic composition according to claim 1, wherein the date extract is extracted by hot water extraction. The cosmetic composition of claim 1, wherein the cosmetic composition has a formulation selected from the group consisting of flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. Cosmetic composition, characterized in that.