KR20090084117A - Cosmetic composition using shizonepetae herba and nelumbinis semen - Google Patents

Abstract

A mixture herbal medicine composition as a cosmetic functional ingredient is provided to have anti-inflammation, antioxidation, whitening and antibacterial activity and improve skin condition without side effect. A cosmetic composition comprises extracts of Schizonepetae Herba and Nelumbinis Semen. The extracts of Schizonepetae Herba and Nelumbinis Semen is mixed in a same ratio. The extract is obtained by dipping the Schizonepetae Herba and Nelumbinis Semen in 70% ethanol. The extract further comprises Mulberry root-bark. The cosmetic composition is used in a form of toner, emulsion, serum, cream type, cleansing product, make-up or powder.

Description

Cosmetic composition using hyunggae, soft meat {Cosmetic composition using Shizonepetae Herba and Nelumbinis Semen}

The present invention relates to a composite herbal composition as a cosmetic functional raw material having an anti-inflammatory, antioxidant and whitening, antibacterial effect, the extract obtained by extracting a certain amount of hyeongjak, lotus root meat exhibits excellent activity of anti-inflammatory, antioxidant and whitening, antibacterial Can be used as

Our human body is in close contact with the external environment and is easily sensitized by environmental pollution, harmful substances, stress, and irregular lifestyles. The skin exposed by such an external environment generates free radicals, that is, free radicals, and the generated free radicals and free radicals attack normal cellular tissues or cell membranes, resulting in cell destruction and skin eventually causing inflammation. Inflammatory tissues are the main causes of skin problems as well as atopic dermatitis and skin aging by destroying proteins and genetic material by physiological mechanisms of the skin. In addition, when the skin is overexposed to ultraviolet rays, the action of tyrosinase promotes melanin synthesis in melanosomes, leading to rapid skin aging, and the resulting free radicals damage lipids, proteins, sugars and nucleic acids, and are involved in the destruction of cell membranes. Cause skin cancer.

In order to improve the problems of the skin condition, it is important to improve the surrounding living environment first, but various methods are necessary to normalize the skin that has additionally lost its function. Therefore, cosmetics containing various herbal ingredients that help improve skin condition have been developed and used to maintain healthy skin. However, using cosmetics that contain herbal medicines that have not been accurately identified can adversely affect consumers, so it is necessary to verify the various activities related to the skin of these herbal medicines before applying them to cosmetics.

Thus, the present invention focused on the development of raw materials of functional cosmetics that can help skin trouble and aging due to harmful environment, namely environmental pollution, ultraviolet rays, harmful substances, stress, irregular lifestyle, etc., which are easily encountered in daily life. Herbal medicines were selected based on the prescription of herbal medicines, especially Dongbobogam, which can be easily used around the skin and help improve skin condition without accompanying side effects. In addition, after extracting by various methods, suitable extraction methods for cosmetics were selected, and the extracts were prepared by concentration, and the in vitro antioxidant and anti-inflammatory, antibacterial and whitening activities were measured to examine the possibility of excellent cosmetic functional ingredients.

The object of the present invention is achieved by an extract of the same ratio of hyeongjak, soft and soft.

In the present invention, the extracts of the same ratio of hyungjak and lotus roots are anti-inflammatory, antioxidant and whitening, antibacterial activity.

The present invention is a cosmetic composition showing the anti-inflammatory, antioxidant and whitening, and antimicrobial activity of the extract and mixture of lotus root and lotus root in the same ratio.

Here, a mixed extract of an additional mixture of baekbaekpi, which is often used as a raw material for whitening cosmetics may be used.

Mold opening used in the present invention ( Schizonepeta tenuifolia Briq.) is a herbaceous herb that belongs to Labiatae, warm in nature, tastes very bitter, and has no poison. In oriental medicine, the ground of the penis has been used as a treatment for cold fever, sore throat, margin, postpartum stroke and hypothalamus, and hemostatic agents. Antiplatelet aggregation and immunoglobulin bioprotective activity has been found in mice. Flavonoids and essential oils are the main components of the mold, and flavonoids are excellent for alleviating skin inflammation and sedating. Essential oils consist of d -menthone, l -pulegone, and sesquiterpene, 3-octanone, 3-octanol, such as monnterpene such as l -isomenthone, d -limonene, isopluegone, piperitenone, and caryophyllene, β-clemene, β-humulene, etc. l -octun-3-ol.

Lotus root is a seed removed from the bark of the mature fruit of the perennial aquatic plant lotus ( Nelumbo mcifera Gaertner) belonging to the Nymphaeaceae, tastes sweet and its nature is plain. It's called lotus or yeonjang, and it has been widely used in medicine and edible since ancient times. The name of the lotus is different depending on where it is used. The leaf is lower lobe, the root is falcon, the fruit is federal, the pistil is soft, and the green embryo in the seed is called soft core. Yeonjagi is mainly used to treat hemostasis, blood removal, haemostasis, hemostasis, hematuria, bloody stool in oriental medicine. The main components of lotus root meat are aporphine alkaloids such as nuciferine, N -nornuciferine, O -nornuciferine and roemerine, and phenolic alkaloids such as liensinine, isoliensinine and neferine and (+)-1 (R) -coclaurine, (-)-1 ( There are benzylisoquinoline alkaloids such as S) -norcoclaurine, and flavonoid compounds such as (+)-catechin, (+)-gallocatechin, quercetin and kaempferol and amino acid components such as tryptophan, asparagine and tyrosine.

Morus root-bark, Mori Radicis Cortex, Morus alba L.) or other related plants (Moraceae), the root bark, cold, sweet and nontoxic. Antipyretic, anticonvulsant, anti-allergic, anti-inflammatory, diuretic, whitening, etc. The main physiologically active ingredients are morusin, cyclomorusin, sanggenon A ~ E, kuwanone A, B, C, α-amyrin, β-amyrin , Fatty acids and the like.

The herbal medicines used in the present invention have been reported in various literatures and articles that are very effective and effective against various skin diseases that can be experienced in everyday life. Therefore, the anti-inflammatory and antioxidant effects can be confirmed by various in vitro experiments based on the above herbal medicines.

Herbal medicines used in the present invention is the type of hyeongjak: softly mixed with the same amount, immersion extraction at room temperature for 24 hours using water and ethanol mixture (30: 70, w / w), filtered and concentrated to the final extract Got it. Comparative extracts were also prepared in the same manner.

The herbal extract of the present invention can be used in various cosmetic compositions characterized by anti-inflammatory and antioxidant effects.

The extraction method of the herbal medicine extract, in vitro antioxidant and anti-inflammatory, antibacterial, whitening effect of the herbal medicine extract was described in more detail through the following preparation examples, experimental examples.

Extraction Method

Preparation of Extract 1 Test group , HY ):

After grinding 15g of each mold and soft porridge, 300ml of 70% ethanol was added and the mixture was stirred and extracted at room temperature for 24 hours. The extract was concentrated to 1/10 after filtration.

Preparation of Extract 2 ( Test group , HS ):

After grinding 15g of hyungpyeong, yeonjak, baekryepi each, and then put 450ml of 70% ethanol and extracted by stirring for 24 hours at room temperature. The extract was concentrated to 1/10 after filtration.

Experiment method

Experiment 1. DPPH On by Free radical  Reduction Activity by the Elimination Method

In order to test the antioxidant effect of the herbal extracts, the electron donating ability was measured and compared with the control group. The 1,1-diphenyl-2-picryl-hydrazil (DPPH, D9132, Sigma, USA) used in the measurement of electron donating ability is a stable free radical, and if the sample has antioxidant activity, the lipid oxidation of DPPH By eliminating the non-covalent bonds of free radicals involved in the DPPH will reduce the reducibility of DPPH, the more purple DPPH is reduced, the more purple is lost, the lower the value when measuring UV. When DPPH receives electrons or hydrogen, its absorbance decreases near 517 nm, and if each extract has a high ability to reduce or offset these radicals, high antioxidant activity and scavenging activity against other radicals including free radicals can be expected. It can also be used as a measure of suppressing aging by active radicals. DPPH was prepared at 0.2 mM, and samples prepared by concentration were added to 1 mL of DPPH solution. After reacting at room temperature for 10 minutes, the absorbance was measured at 517 nm with a UV-Vis spectrophotometer (spectra plus 384, molecular device, USA). For the control group, ethanol was added instead of the sample solution and the DPPH solution to obtain a correction value. Free radical scavenging rate was calculated according to the following formula. The results are shown in FIG.

% Clearance = (absorbance of control group-absorbance of test group) / absorbance of control group X 100

The control group used 6-hydroxy-2,5,6,8-tetramethylchroman-2-carboxylic acid (Trolox, 97%, Aldrich, USA). Trolox is a vitamin E analogue that is highly antioxidant. In the test, samples diluted with 0.1% concentration in ethanol and having similar or higher values to trolox based on 50% of activity were considered to have good activity. Extract 1 was found to have higher activity than extracts 2 and 3, and activity was not increased even when three or more composite materials were used. Extract 1 was diluted in 1/2, 1/5, 1/10 concentration to confirm the effect, there was no difference in activity. The results are shown in Table 1.

Table 1. Extract 1 ( HY )of Free radical  Elimination effect (n = 5)

concentrate 1/2 1/5 1/10 Trolox mean ± D (%) 91.32 ± 0.79 92.12 ± 0.02 91.89 ± 0.02 91.55 ± 0.36 50.27 ± 0.03

Experiment 2. SOD Pseudo-activity  Measure

In order to test the antioxidant effect of the herbal extracts, the effects of the herbal extracts were determined by measuring the SOD-like activity as well as the electron donating ability. Reactive oxygen species SOD activity is determined by the activity of NBT (nitrobluetetrazolium, Sigma, USA) by reactive oxygen using a reactive oxygen generator by xanthine / xanthine oxidase (Sigma, USA) enzyme reaction. The absorbance change due to oxidation was measured and evaluated. 0.05 mM Na ₂ CO ₃ (Mw 105.99, Yakuri Pure Chemicals Co., Ltd, Japan) 2.4 ml, 3 mM xanthine (X-0626, Sigma, USA) 0.1 ml, 3 mM EDTA (E-5134, Sigma, USA) 0.1 ml , 0.1 ml of BSA (bovine serum albumin, A-7906, Sigma, USA), 0.1 ml of 0.75 mM NBT (N-6875, Sigma, USA), and 0.1 ml of sample were mixed well and allowed to stand at 25 ° C. for 10 minutes. 0.1 ml of xanthine oxidase (X-4376, Sigma, USA) was added and reacted at 25 ° C. for 20 minutes. Then, 0.1 ml of 6 mM CuCl ₂ (Daejung Chemicals & Metals Co., LTD, Korea) was added to stop the reaction. Absorbance was measured at 560 nm with a visible light spectrophotometer (spectra plus 384, molecular device, USA). In the control group, purified water was added instead of the sample, and purified water was added instead of xanthine oxidase to obtain a color correction value. Scavenging rate was calculated according to the formula used for DPPH radical scavenging rate. The results are shown in FIG.

The herbal extract was compared with the control vitamin C (L-ascorbic acid, A-1417, Sigma, USA) was confirmed the antioxidant effect. The activity was based on 60% to 80% and was considered to be high when the value was similar or high. Extract 1 has higher activity than extracts 2 and 3, and as in the result of Experiment 1, it was confirmed that the activity does not increase even when using three or more composite materials. In addition, extract 1 had high activity regardless of the concentration decrease (diluted by 1/2, 1/5, 1/10). The results are shown in Table 2.

Table 2. Extract 1 ( HY )of SOD  Pseudo-activity (n = 5)

concentrate 1/2 1/5 1/10 Vitamin c mean ± D (%) 96.24 ± 0.88 97.33 ± 0.35 92.67 ± 0.56 98.67 ± 0.78 60.94 ± 0.20

Experiment 3. Tyrosinase  Inhibitory activity test ( In in vitro tyrosinase inhibition assay )

To test the whitening effect of the herbal extracts, tyrosinase inhibitory activity was measured and compared with the control group. When the skin is exposed to ultraviolet rays, the action of tyrosinase promotes skin aging by synthesizing melanin from melanosomes.Tyrosinase is an enzyme involved in the initial rate determining step of the melanin biosynthesis pathway in the human body. Has a mechanism of action. This test is a method of evaluating the degree of inhibition of tyrosinase enzyme activity in a sample. 0.9 ml of sample, 1.0 ml of 0.1 M phosphate buffer (pH 6.5) and 1.0 ml of 1.5 mM L-tyrosine (T8566, Sigma, USA) were added, and then allowed to stand at 37 ° C for 10 minutes. After 0.1 ml of mushroom tyrosinase (T3824, Sigma, USA) was added and reacted at 37 ° C. for 10 minutes, the absorbance was measured at 480 nm using a UV-Vis spectrophotometer (spectra plus384, molecular device, USA). The tyrosinase activity inhibition rate was calculated according to the following formula. The results are shown in FIG.

% Inhibition of tyrosinase activity = (absorbance of control group-absorbance of test group) / absorbance of control group X 100

To evaluate the whitening activity, the activity was compared using 2% arbutin as a control. The activity was based on 60% to 80% and was considered to be high when it had a similar or higher value. Extract 1 had high activity regardless of the concentration decrease (diluted to 1/2, 1/5, 1/10). The results are shown in Table 3.

Table 3. Extract 1 ( HY )of Tyrosinase  Inhibitory activity (n = 5)

concentrate 1/2 1/5 1/10 Arbutin mean ± D (%) 89.88 ± 0.88 82.25 ± 0.355 87.00 ± 0.06 86.88 ± 0.42 73.63 ± 0.53

Experiment 4. Lipoxygenase ( Lipoxigenase Anti-inflammatory test by inhibition of activity

In order to evaluate the anti-inflammatory effect of the extract, lipoxygenase inhibitory activity was measured. The inhibitory effect of lipid peroxide production was determined by measuring linoleic acid (L2376, Sigma, assay 98%, USA) as a substrate by measuring the peroxide value, an intermediate product of oxidation of unsaturated fatty acids. 1 ml of linoleic acid, 0.1 ml of sample, and 0.9 ml of lipoxygenase (L7395, Sigma, USA) were mixed well at a constant rate and time for 10 minutes at 25 ° C., followed by 20% w / v trichloroacetic acid (TCA , Junsei Chemicals Co., Ltd, Japan) 0.5ml, 0.6% w / v Thiobarbituric acid (TBA, T5500, Sigma, USA) was added and mixed by heating in boiling water for 10 minutes and then cooled. 2 mL of butanol was added to the cooled sample, mixed with a vortex mixer for 20 seconds, and centrifuged at 3,500 rpm for 5 minutes to obtain a supernatant. Absorbance is measured at 535 nm using a UV-Visible spectrophotometer (spectra plus 384, molecular device, USA). The activity inhibition rate is calculated according to the following formula. The results are shown in FIG.

% Inhibition of lipoxygenase activity = (absorbance of control group-absorbance of test group) / absorbance of control group X 100

Unlike the experiments 1, 2 and 3, the extract was relatively concentration dependent on anti-inflammatory activity. As the amount of 1/2 decreased, the activity decreased by 28% and the concentration was decreased by 10 times, indicating no activity. The results are shown in Table 4.

Table 4. Extract 1 ( HY Anti-inflammatory activity of) (n = 5)

concentrate 1/2 1/5 1/10 mean  ± D (%) 63.09 ± 0.91 45.43 ± 2.03 42.89 ± 0.17 ㅡ

Experiment 5 Disc Diffusion ( Disc diffusion Antibacterial activity

The antimicrobial activity of the extract was measured by Disc diffusion method. Gram-positive strain Staphylococcus aureus KCTC 3881, Pseudomonas as Gram-negative bacteria aeruginosa KCTC 1750, Candida albicans as yeast KCTC 7965, Aspergillus as fungus niger KCTC 6196 was used for the experiment. In other words, each strain cultured in a plate medium was incubated with platinum and cultured in 10 ml of liquid medium for 18 to 24 hours to activate, and then inoculated with 0.1 ml of bacterial solution in the liquid medium and incubated for 3 to 6 hours. The fungus solution was inoculated in saline to about 10 ⁶ to 10 ⁷ cells per plate medium and evenly spread with a sterile swab. Place the sterilized paper disc on a solid plate medium and absorb the sample by concentration to 0.05ml / disc. Incubate the bacteria at 37 ℃ for 24 hours, yeast at 25 ℃ for 48 hours, and mold at 25? For 72 hours. The surrounding clear zone was confirmed. The results are shown in FIG. 5. Experimental results showed no antimicrobial activity against yeasts and molds and bacteria Staphylococcus aureus , Pseudomonas Antimicrobial activity was confirmed by showing clear clear zone against aeruginosa .

As a result of in vitro experiments, it is possible to show all the activities of antioxidant, whitening, anti-inflammatory, and antimicrobial even with only hyungjeo and yeonjak (HY) among the constituents. Not found. Therefore, extracts including mold opening and lotus root meat can be applied as a cosmetic raw material.

The medicine has free radical scavenging effect and free radical scavenging effect and can show effects on anti-aging and anti-oxidation. It can also be added to skin trouble improvement products on the basis of anti-inflammatory and antimicrobial activity and can be applied to products that combine these functions.

1 is a graph showing the free radical scavenging effect of the herbal extract of the present invention

Figure 2 is a graph showing the SOD-like activity of the herbal extract of the present invention

Figure 3 is a graph showing the tyrosinase inhibitory activity of the herbal extract of the present invention

Figure 4 is a graph showing the lipoxygenase inhibitory activity of the herbal extract of the present invention

Figure 5 is a photograph showing the antimicrobial activity of the herbal medicine extract of the present invention

Claims (6)

Cosmetic composition containing the extract of Hyeonggae and lotus root meat. The cosmetic composition according to claim 1, wherein the mold extract and lotus root extract are mixed in the same weight ratio. The cosmetic composition according to claim 1 or 2, wherein the extract is an extract obtained by immersing the open and soft muscles in 70% ethanol (water: ethanol = 3: 7, w / w). The cosmetic composition according to claim 1, wherein the extract further comprises lettuce. The cosmetic composition according to claim 4, wherein the extracts of Morus alba L., H. C. and Yeonja meat are mixed in the same weight ratio. The cosmetic composition according to claim 1, wherein the cosmetic composition has a formulation of toner, emulsion, serum, cream, cleansing agent, makeup or powder.

Images