KR20090052652A - Composition for preventing gray hair and for treatment of leukoplakia containing arrowroot - Google Patents

Abstract

The present invention discloses a composition for preventing white hair and for treating vitiligo, containing the root of root as an active ingredient. The composition for preventing and treating vitiligo according to the present invention increases the MITF expression of melanocytes, activates melanocytes, and promotes melanin synthesis, thereby promoting the treatment of vitiligo and vitiligo.

Brown root, skin, melanocyte, MITF, melanin, white hair, vitiligo

Description

Composition for preventing gray hair and treating vitiligo containing reeds {Composition for preventing gray hair and for treatment of leukoplakia containing arrowroot}

The present invention relates to a composition for preventing alveolar and vitiligo containing the roots as an active ingredient, specifically, to increase the MITF expression of melanocytes, to activate melanocytes and to promote melanin synthesis, to prevent vitiligo and vitiligo It relates to a composition for preventing white hair and treating vitiligo.

Since white hair generally occurs as aging progresses, it gives a strong impression of old age. Therefore, a lot of dyes that blacken white hair have been studied in order to hide the generated white hair. However, dyeing should be dyed again by the growth of white hair in a temporary manner, and oxidative black hair dyes, which are widely used at present, damage the skin by the use of oxidants.

As a white hair suppressant, black sesame seeds, rosacea, nasturtium and honey can be used as a supplementary ingredient to Sukji Hwang, Cheonmun-dong, and McMung-Dong. Put together in a container and boil at 50 ℃ temperature to keep the black soybeans in black liquid, black sesame seeds, black pine leaves, using kelp, oggal extract extract, using pituitary hormone, C-AMP (adenosine- 3,5 'cyclic phosphate), vitamin D3, etc. have been disclosed, but the effect that can be felt is not exhibited.

The present inventors observed that brown root of various natural substances increases the melanocyte production by increasing the MITF expression of melanocytes and inhibits the progression of white hair when applied to vitiligo model mice. Therefore, an object of the present invention is to provide a composition for preventing white hair and treating vitiligo, which contains brown root as an active ingredient.

The present invention provides a composition for preventing white hair and a composition for treating vitiligo, containing the roots as an active ingredient.

In the composition of the present invention, the composition may promote melanin production by promoting the expression of MITF of melanocytes.

The composition for the prevention of white hair and vitiligo containing the root of the root (칡) as an active ingredient according to an embodiment of the present invention, by increasing the MITF expression of melanocytes to activate melanocytes and promote melanin synthesis to prevent white hair, When applied to vitiligo model mice, it suppresses the progression of white hair.

The cause of the white hair has been the stem cell loss of melanocytes and the decrease in the activity of melanocytes. In particular, the white hair due to aging is mainly caused by the loss of stem cells, and the occurrence of white hair including sachets is known to be due to the deactivation of melanocytes caused by environmental and mental stress in modern society.

The melanin synthesis activity of melanocytes is greatly influenced by the activity of MITF, and the root-containing composition according to an embodiment of the present invention can significantly suppress the expression of MITF in melanocytes and inhibit white hair.

When the composition according to an embodiment of the present invention is applied to cosmetics, it may contain a cosmetically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase, and / or It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. These compositions can be prepared according to conventional methods in the art. In particular, the composition can be easily prepared in the form of a shampoo, conditioner, conditioning, tonic or scalp essence applied to the scalp or hair. Compositions according to one embodiment of the invention are fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, blowing agents, fragrances, surfactants, water, ionic or nonionic Cosmetics such as emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics It may contain adjuvants commonly used in the art. These adjuvants are introduced in amounts generally used in the cosmetic field.

When applying the composition according to an embodiment of the present invention to medicines, parenteral administration in the form of solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier with the root of the root (칡) as an active ingredient (transdermal administration Or external coating). Preparations for parenteral administration include ointments, lotions, sprays, suspensions and the like. In order to formulate the active ingredient according to an embodiment of the present invention, it can be easily formulated according to the conventional method, and a surfactant, an excipient, a coloring agent, a spice, a preservative, a stabilizer, a buffer, a suspending agent, and other commonly used auxiliaries can be suitably used. Can be.

Dose of the root-containing composition according to an embodiment of the present invention is determined by the age, sex, weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration and the judgment of the prescriber. Will depend. Dosage determination based on these factors is within the level of one of ordinary skill in the art and can be applied at the level of one of skill in the art by external application to areas where white hair or vitiligo develop. Generally dosages range from 0.001 mg / kg / day to approximately 2000 mg / kg / day. Preferred dosages are 200 μg / kg / day to 5 mg / kg / day.

Hereinafter, preferred examples and test examples of the present invention will be described in more detail. However, the following embodiments are merely preferred embodiments of the present invention, and the present invention is not limited to such embodiments.

[Test Example 1] Test of melanin production promoting effect of brown root

In the medium to which 5% fetal bovine serum, 100 IU penicillin G and 0.2 uM TPA were added to RPMI medium, melan a cells were dispensed to 50,000 cells / well in a 24-well microtiter plate. The next day, the divided cells were treated with a test compound of root root (1000x stock) at a final concentration of 10 and 50 ppm, treated with 0.1% DMSO as a negative control and 100 uM IBMX as a positive control, followed by 37 ° C temperature. Incubated for 3 days. After incubation, the wells were washed with PBS, and 100 μl of 1N NaOH was added to dissolve the melanin in the cells. The absorbance of the dissolved melanin was measured at 405 nm using a microplate reader. According to Figure 1 showing the effect of promoting the melanin production of the brown roots compared to the control group, the brown roots promote the melanin synthesis of melanocytes increased melanin production, especially at 50ppm promote melanin production higher than the positive control group Effect.

[Test Example 2] The effect of promoting the expression of MITF and tyrosinase in melanocytes of brown root

Dispense 500,000 cells / well in a 6-well microtiter plate using a 501mel cell line, in each ball 0.1% DMSO as a negative control, IBMX 100 uM as a positive control, and as a test group. Brown root was treated with 10 ppm and incubated at 37 ° C. for 24 hours, 48 hours, and 72 hours to obtain protein. The protein thus obtained was subjected to western blot using MITF and tyrosinase antibody. Protein extraction and Western blot were typically performed by standard methods used by those skilled in the art. The results after Western blot are shown in Figure 2a (Western blot band picture) and Figure 2b (protein quantitative graph) compared to the negative control. According to Figure 2, the root muscle increased the expression of MITF and tyrosinase protein in melanocytes.

Experimental Example 3 Evaluation of Efficacy of Brown Hair Inhibition of Roots in Vivo Using Leukemia-Promoting Leukemia Mouse

Vitiligo mice (C57bl / 6- Mitf ^{mi - vit} ) were purchased from The Jackson Lab, USA. Experimental method for the effect of white hair prevention material using the mouse to promote the development of white hair is as follows. Dorsal hairs of 12-week-old mice are removed by depilation. However, the area of hair removal area is adjusted equally for each individual. From the day after the hair is removed, apply anti-hair white matter to the area where the hair is removed twice a day. As a vehicle of the anti-harvest material, a mixture of EtOH: 1,3-BG: DW = 3: 2: 5 (volume ratio) was used, and the carrier was used as a negative control. As a control group, a solution to which 2.5% of root roots were added was used as a test group. After about three weeks, when the difference in anti-white hair efficacy between each substance is visually identified, newly grown hairs are collected and the amount of melanin in the hair is measured. The amount of melanin in the hair is measured using the proteinase enzyme Esperase (Novozyme). A reaction buffer was prepared by dissolving the esperase to a concentration of 1 NPU / ml in a buffer (50 mM Tris-HCl, 5 mM DTT, pH 9.3). 5 mg of mouse hair was added to 1 ml of the reaction buffer, followed by reaction for 13 hours while shaking at a speed of 1,000 rpm at 37 ° C., and the hair and the reaction solution were separated by instant centrifugation. The reaction solution thus obtained was placed in a 96 well plate, and the absorbance at 405 nm was measured to determine the amount of melanin in the reaction solution.

Figure 3 shows the result of measuring the efficacy of the negative control group, positive control group, test group material in the vitiligo mouse model promoted leukemia development by visual observation and melanin quantification method in the hair. Figure 3a is a photograph of the appearance of the mouse hair grown when applied for 3 weeks anti-white hair candidate material, Figure 3b is a graph measuring the melanin amount of the hair grow. According to FIG. 3, brown root suppressed white hair and increased the amount of melanin in hair in mice in which white hair development was promoted.

The composition according to an embodiment of the present invention may be prepared by the following preparation example, but is not limited thereto.

Production Example 1 Hair Shampoo

Ingredient Name weight % Sodium Lauryl Sulfate Solution (30%) 20.0 Palm oil fatty acid diethanolamide 5.0 Polyquaternium-10 0.3 Propylene glycol 2.0 Brown root 0.1-2.5 Pyrotonolamine 0.5 Yellow 203 Quantity Paraoxybenzoic Acid Ester 0.2 Combination Quantity Citric acid Quantity Purified water Remaining amount

Preparation Example 2 Hair Conditioning

Ingredient Name weight % Cetyltrimethylammonium chloride (29%) 7.0 Distearyl dimethylammonium chloride (75%) 4.0 Cetostearyl alcohol 3.5 Polyoxyethylene stearyl ether 1.0 Liquid paraffin 2.0 Propylene glycol 1.5 Brown root 0.1-2.5 Combination Quantity Citric acid Quantity Purified water Remaining amount

Preparation Example 3 Scalp Hair Tonic

Ingredient Name weight % menthol 0.1 D-panthenol 0.6 Salicylic acid 0.05 glycerin 1.0 Polyoxyethylene Cured Castor Oil 0.8 Tocopherol Acetate 0.03 Combination Quantity Brown root 0.1-2.5 ethanol 30.0 Purified water Remaining amount

Preparation Example 4 Scalp Essence

Ingredient Name weight % ethanol 30.0 Polysorbate 60 1.5 glycerin 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Brown root 0.1-2.5 antiseptic A reasonable amount Spices and Colors A reasonable amount Purified water Remaining amount

 Production Example 5 Ointment

Ingredient Name weight % Caprine / Capryl Triglycerides 10 Liquid paraffin 10 Solbitan Sesquioleate 6 Octyldodeceth-25 9 3,7 hexanoate 10 Squalane One Salicylic acid One glycerin 15 Sorbitol One Brown root 2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Production Example 6] Lotion

Ingredient Name weight % Brown root 0.1-2.5 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservatives, Colors and Flavors Quantity Purified water Remaining amount

1 is a graph showing the melanin production promoting effect of the roots.

Figure 2a is a Western blot band picture showing the synergistic effect of MITF and tyrosinase expression of the roots.

Figure 2b is a protein quantitative graph of the effect of MITF and tyrosinase expression of the roots.

Figure 3a is a photograph of visual observation of the effect of the white hair of white roots in vitiligo model mouse.

FIG. 3B is a graph showing the melanin amount in the hair showing the effect of white hair on the hair roots in vitiligo model mice.

Claims (8)

A composition for preventing white hair, containing brown root as an active ingredient. The composition for preventing hair loss of claim 1, wherein the composition promotes melanin production of hair by promoting expression of MITF of melanocytes. The composition for preventing hair loss of claim 1, wherein the composition promotes melanin production of hair by promoting expression of tyrosinase of melanocytes. The composition for preventing white hair according to any one of claims 1 to 3, wherein the composition is applied to the scalp or hair. According to claim 4, wherein the composition is anti-hair care composition having a formulation of shampoo, rinse, conditioning, tonic or scalp essence. Vitiligo treatment composition containing the root of the root as an active ingredient. The composition for treating vitiligo according to claim 6, wherein the composition promotes melanin production by promoting expression of MITF of melanocytes. The composition for treating vitiligo according to claim 6, wherein the composition promotes melanin production by promoting the expression of tyrosinase of melanocytes.

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