KR20090021644A - A carbohydrate and lipid absorption inhibitory nelumbo composition and its manufacturing process thereof - Google Patents

Abstract

A composition for obesity suppression and treatment including leaves extract of nelumbo nucifera is provided to facilitate provision of raw materials for a herbal medicine with low cost and ensure an effect of suppressing absorption of carbohydrate and lipid. A composition for obesity suppression and treatment including leaves extract of nelumbo nucifera comprises 140-180wt% of leaves extract of nelumbo nucifera, 30-50wt% of Cinnamomi Ramulus, 20-40wt% of ginger, 80-100wt% of Coicis semen, 30-50wt% of Polygoni Multiflori Radix, 20-40wt% of Phaseolus angularis Wight, 20-30wt% of Saliconia herbacea, 15-25wt% of dried chestnut shell, 10-30wt% of cottonseed, 30-50wt% of Raphani Semen, 30-40wt% of Atractyodis Rhizoma, 15-25wt% of Atractylodes macrocephala, 1-7wt% of Zingiberis Rhizoma, 30-40wt% of Crataegi Fructus, and 15-25wt% of extract obtained by extracting licorice using alcohol.

Description

Herbal medicine composition having carbohydrate and fat absorption inhibitory activity as a main ingredient and manufacturing method thereof {A CARBOHYDRATE AND LIPID ABSORPTION INHIBITORY NELUMBO COMPOSITION AND ITS MANUFACTURING PROCESS THEREOF}

The present invention relates to a herbal composition comprising a lower leaf extract as a main component and a manufacturing method using the same.

Obesity is an overweight condition in which men have more than 25% of their body fat and 30% of their body fat. Clinically, it is defined as the case of Body Mass Index (BMI) divided by the height (M) squared more than 30 (Korean Society of Obesity: Translation and Diagnosis: Obesity Diagnosis and Treatment, Seoul, Korea. 2003; Garrow JS: Obesity and related Diseases, Edinburgh, Churchill Livingstone, 1988).

Obesity is one of the most dangerous and lethal diseases in the 21st century. The reason for this is that obesity is dangerous in itself, but the pressure on the abdomen by fatty tissues often causes constipation, indigestion, gastrointestinal disorders, and also significantly increases the risk of various other diseases. . Diseases that increase the risk of obesity are typically type 2 diabetes, hypertension, coronary artery disease, stroke, and various cancers.

There are 300 million adult male and female obesity patients worldwide, and the United States has the highest proportion, with over 65% of adults being obese. In addition, 300,000 people die each year from obesity-related diseases (Allison DB et. Al: prevalence & Trends in Obesity among US adults, 1999-2000, JAMA 288: 1723-7, 2002; WHO, Obesity: Preventing & Managing Global Epidemic , Report of WHO Consultation on Obesity, 3-5 June, 1997).

This is due to the fact that people store excess calories in the form of fat, and thanks to economic development and advances in technology, new eating cultures, especially eating out, have increased intake of fat and sugar while reducing fiber intake. The main reason for this is that the modern lifestyle, which has little physical activity, increases the sitting time and makes it easier to obtain cheap high-fat foods.

Obesity has exploded in the same generation, and diet, exercise, or lifestyle changes alone cannot solve it. Therefore, the necessity of solving the obesity problem is increasing through medicines and nutraceuticals.

Anti-obesity drugs can be used to treat obesity. However, commercially available anti-obesity drugs have a problem in that side effects appear. To date, it is well known that many products used for obesity medication have side effects and have many social problems.

For example, dexfenfluramine and fenfluramine are serotonin reuptake inhibitors that have been approved by the US Food and Drug Administration (FDA) but have been found on the market in October 1997 because of heart valve abnormalities. Fluoxectin has been reported to have side effects such as allergic rash, nausea and insomnia, and insomnia, headache, nausea, diethylpropion, phentermine and mazindol. Side effects such as thirst have been observed. In addition, there are reports of side effects in the cardiovascular system of sibutramine and gastrointestinal side effects of orlistat (Xenical), a prescription drug for obesity drugs, which has recently been exploding in sales (Fujioka K et al. Diabetes Obes . Metab . , 2 (3) , pp 175-187, 2000; Leung WYS et al., Clinical Therapeutics , 25 (1) , pp 58-80, 2003; McMahon FG et al., Arch . Int . Med . , 160 (14) , pp 2185-2191, 2000).

In particular, sibutramine (sibutramine, Reductile) induces a feeling of satiety to suppress appetite, but not only is expensive, but also exhibits side effects of hyperactivity and insomnia and sexual desire suppression. In addition, Olistat, a specialist drug for the treatment of obesity, which has been exploding in recent years, is a fat metabolism inhibitor that inhibits the breakdown metabolism enzymes of fatty acids and glycerides, which are components of fat molecules, by about 20%. Fat molecules have the effect of being discharged as it is, but also has the disadvantages of expensive, fatty stools appear and lack of intake of fat-soluble vitamins

Therefore, the development of a new functional material without side effects for the improvement and prevention of obesity is required. In addition, ephedrine (ephedra) preparations have the disadvantage of mental stress due to excessive food intake.

However, even when taking into account the side effects, the effects of these medicines are about 5 to 10% weight loss effect, far below the expected target of the patient or doctor 15 to 20%. Therefore, there is a growing demand for obesity-related medicines that are effective in weight loss and that can reduce the risk of side effects and obesity-related diseases. While the statistics show fewer obesity drugs than other long-term prescription drugs, experts predict that the market will reach $ 3.7 billion in 2008 when new, safe and effective drugs are created.

In addition to the drugs, products of herbal ingredients are being developed and marketed. That is, the focus is on the consumption of drugs, diet foods and health functional foods derived from natural materials that have an effect on suppressing obesity and improving constipation. In particular, Cois semen, Dioscoreae rhizoma, Platycodi radix (flower bellflower), Schizandrae fructus, Citrus unshiu Markovich, Aloe vera, Adenophorae radix (Sasam), etc. Some anti-obesity drugs have been developed, which consist of extracts of several herbal drugs or mixtures.

However, in the case of products derived from such natural materials so far known, the material is very expensive, in order to obtain a proper effect in many cases, or in fact, the effect of suppressing obesity and preventing hyperlipidemia was often insignificant. In addition, there is virtually no compound drug or herbal product that has no obvious effects and side effects on obesity.

Therefore, there is a need to research and develop a new herbal composition that can be easily obtained even though it is a natural material and can have a more complex effect.

In order to solve the above problems, an object of the present invention is to provide a herbal composition that is inexpensive, easy to procure raw materials, and exhibits an excellent anti-obesity effect.

In another aspect, the present invention is to provide a pharmaceutical and food containing the herbal composition for inhibiting obesity.

That is, an object of the present invention is to provide a herbal composition that can effectively prevent and suppress obesity and related diseases.

In order to achieve the above object, the present inventors have scientifically reviewed and analyzed many traditional herbal medicines known to inhibit the digestive absorption and metabolism of carbohydrates and fats, directly or indirectly, to prepare herbal compositions showing the best anti-obesity prevention effect. .

The composition of the obesity inhibitor herbal composition of the present invention is shown in Table 1 below.

Herbal Medicine for Extraction Composition ratio (part by weight) Lower leaf (lotus leaf) 140-180 Cock 30-50 ginger 20-40 Righteous 80-100 Droopy 30-50 Small cow 20-40 Hamcho 20-30 Dry rate 15-25 Cotton yarn 10-30 A fugitive 30-50 Creation 30-40 Whiteness 15-25 health  1-7 Sansa 30-40 licorice 15-25

Hereinafter, each component of the composition of the present invention will be described in detail.

The lower lobe refers to the leaves of the lotus, which is a water lily and a plant. The lotus (Nelumbo nucifera) is a perennial herb, and the rhizome is thick and long and many nodes. The lower lobes are long, lobes are long, appear above the water, and do not have serrations in the shape of a round shield. And the leaves have short, sharp spines. The lower lobe has diarrhea, headache and dizziness, enuresis and detoxification (Yook Chang-soo, Colored Medicinal Plants of Korea, 1990, Academy Books, pp 210-230). Ingredients contained in the lower lobe include roemerine, nuciferin, armepavine, n-nornuciferine, and pronuciferine.

Lower lobe has been reported to reduce oral and lipid concentrations that may have been fed to high-fat dieted white mice by affecting lipid metabolism in vivo (Shin Mi-kyung, et al., 21 (2), 202-208,2006). . A paper on lipid lowering effects has also been published in hamsters (Seongbin Kim et al., Journal of Pharmacognosy, 36 (3) 229-234,2005).

In ancient literature, such as Shandong Chinese medicine, the lower lobe raises yanggi and releases bad blood. Therefore, it is said that it is used to vomit blood or sputum mixed with blood, postpartum haemorrhage (瘀血) in the body, pain caused by bruises, etc., and it is said to treat edema by releasing moisture.

Uiin (인 仁, Coicis semen ) is a perennial plant belonging to the family of rice and dried Yulmu's seed. Euiin is effective for swelling, neuralgia, rheumatism, bladder stones, etc. It is used for medicines such as edema, neuralgia, rheumatism and bladder stones. (Sanchez-Medina et al, Phytomedicine, 8 (2), 144-151,2001).

Uiin tastes sweet and slightly cold, and acts on parenteral and menopause. Carbohydrates, proteins, fats, calcium, iron, ash, vitamins B1, B2, nicotinic acid, and evenly contained, the acetone extract contains tumor suppressing ingredients. Anti-cancer, anti-inflammatory, cholesterol-lowering, sedative and analgesic effects have been found. It should be used with caution in pregnant women (Ji Bo-seop and Shin Min-kyo);

The Cinnamomi Ramulus is a Cinnamomun loureirii NEES) is cut to 15cm in length and dried in the sun, it is used as a herbal medicine. Taste is sour and gentle in nature. It emits abundant winds of the mark, and reaches the limbs, making meridians painless and joints. Make it happen. The action of all over the body has the effect of active blood circulation and acidic pain, so it has an external feeling of cold (풍 感 痛), abundant humidity ratio (寒濕 痺) and menopause (經 閉), It is used to treat symptoms such as pain and pain. It also contains 0.07% of alkaloids, 2.06% of saponins, 2.51% of tannins, and essential oils.

Licorice (甘草, Glycyrrhiza uralensis FISCH.) is a perennial herb of legumes. It is used as an internal or external medicine to alleviate various pain and urgency symptoms as well as to increase efficacy while harmonizing with all herbs in oriental medicine. It has the same weakness as neutralization. For example, licorice has the effect of protecting the liver and facilitating liver function to regulate fatigue, and taking licorice reduces the burden on the liver by its detoxification. Triterpene-based saponins and glycyrrhizin are contained in the roots and roots of plants and have all detoxification effects on bacterial toxins as well as drug addiction, food poisoning, and metabolism in the body. And the amount of thyroid cortical hormone is regulated. Licorice has anti-inflammatory and anti-allergic reactions, antagonistic action against acetylcholine, and a potent effect of adrenaline stimulation. 1998).

The creation acts to remove moist heat (asymmetric heat of the body) and to relax the stomach. Creation is another name for the shovel. In Dongbogam, 'the quality is warm [溫], the taste is bitter [苦], very [辛], there is no poison. Heals the upper, middle and lower rituals, cools the inside, sweats, heals the immersions, the walls, the bizarre and the shogi, and is caused by wind, cold and wet. Vomiting and diarrhea with nausea and swelling are better for inescapables, eliminating tree species and spears. Entering the foot and foot of the Yangyangjing, to strengthen the stomach (健), and to relax the rain (脾). The shovel is magnificent and has a strong ascending force, removes moisture, and stabilizes the rain. '

Saliconia herbacea is a salt plant that grows on the beaches of the west and south coasts of Korea.It has been used as a private herb since ancient times, and it is a plant listed in the Food Code in 2001.It is a useful microelement for humans. Rich in iron

There has been little report on physiological activity except for natural minerals, but recently activities such as antimicrobial activity, antioxidant inhibitor activity, tyrosinase activity inhibition have been reported (Lee, JT and BJ An, Kor J. Herbology). 2002; 17 (2) 61-69).

A legendary drug that was widely used as a medicine for long life in Tang Dynasty.

하 何 오 ( Polygoni Multiflori Radix) is classified as White Sewage and Red Sewage, and White Sewage is commonly used as a medicinal herb in Korea.

  In fact, Baek Sewao and Dung Shui are plants with different plant classifications, and their cultivation methods and processing methods are completely different. Drainage is weak and tastes and writes and acts on the liver and nerves. Blackening the head and stools to work well and to strengthen the heart. In addition, there is also a function to inhibit cholesterol absorption and antibacterial action. Generally speaking, sewage refers to dripping sewage. Baek Ha Shou is a perennial of the genusaceae. It is native to Korea, and it is a plant that grows while rolling up clockwise. When the stem or leaf is cut, white latex comes out. Its main ingredients include starch, crude fat, minerals, lecithin and anthraquinone derivatives. Lecithin strengthens the heart, and anthraquinone derivatives excite the central nervous system and intensify bowel peristalsis, causing weak diarrhea. In Dongbogam, it is used for skin diseases such as constipation, scabies and eczema, and has been used as tonic, gangjeong, and longevity medicine. The plant was originally called Gyo-eung, and it was called sewage because a man named Shaoo ate this herb, blackened his head and lived up to 160 years old.

The pharmacological action of sewage is blood lipid lowering and anti-aging effect. In the old book, sewage was called black north jaw heat color retirement age bulge (廷 年 不 老). Sewage has a pronounced lipid-lowering effect in several animal specimens such as domestic rabbits, pigeons, white mice and quails. It extends the survival time of pigeons and at the same time increases the ratio of plasma HDL-C and HCL-C / Tc, improves lipid metabolism and lowers plasma lipid and oxide content. , ② reduce or prevent atherosclerosis. ③ increase the coronary blood flow to a light degree, and the protective effect of a constant heart muscle. ④ increases the ability to withstand cold mice, ⑤ prevents the thymus atrophy of the old live mice can be seen to be involved in immune function. ⑥ promotes the interlocking effect of the intestinal tract is effective for constipation, ⑦ inhibits the development of Mycobacterium tuberculosis and dysentery. In addition, an anthraquinone derivative is contained among the components of sewage, and the main components are chrysophanic acid, chrysophanol, emodin, rhein, antronene, and starch. According to clinical reports, hyperlipidemia has been reported to be effective after taking 0.25 mg of concentrated solution three times a day for 2 to 12 weeks.

Sansa ( Crataegi Fructus ) is Crataegus , a deciduous tree belonging to the Rosaceae family pinnatifida BGE. var. typica SCHNEIDER.) and dried fruit of the same plant, increasing blood flow in coronary artery, reducing oxygen consumption in myocardium, and protecting against myocardial ischemia and oxygen deficiency. It also increases the blood flow in the coronary arteries and reduces the oxygen consumption and oxygen utilization of the myocardium. It also lowers serum total cholesterol and β-lipoproteins. It also lowers LDL-C and VLDL-C. It also reduces dose-dependent arterial deposition of cholesterol and cholesterol esters. Sansa activates LCAT (lecithine cholesterol acyl transferase), preventing free cholesterol from depositing easily and preventing atherosclerosis. Usolic acid is a component of the treatment of atherosclerosis of the mountain.

These hawthorn can be used for indigestion, hyperlipidemia, hypertension, myocardial infarction, wet dysentery, enteritis, hepatitis, jaundice, menopause, postpartum uterine rehabilitation, and even if the ginseng does not fit, it can be solved with hawthorn.

Raphani Semen is another name for Ms. Shai, which is used to treat seawater (cough) and to calm a swollen boat. Tummy and swelling in the treatment of catching, evenly intestine, and urine out well. In addition, it is said that when you eat it with powdered rice, you will vomit pungdam (風 痰) and the effect is very good. In addition, it is known that moths promote digestion, and are effective in improving symptoms such as indigestion, bloating, planting, and gas in the stomach.

Atractylodes macrocephala ) is the root of large flower indica and cognate green plants. It acts as a diuretic, sweating effect against water metabolism in the digestive tract and subcutaneous tissue, and it is effective in pain, gastroenteritis and edema.It is used as a blood pressure lowering agent in the private sector. .

Red Soybean is Red Bean ( Phaseolus) angularis Wight) or red beans ( Phaseolus calcaratus Roxburgh). In oriental medicine, it is known to be plain and tasteless, sour and non-toxic, to drain water and to suck out a kind of pus, to treat sogal, to diarrhea and dysentery, to pee, and to reduce only the species and the spear. Medicinal Herbal Dictionary, Yeonglimsa Temple, pp698-699, 1998).

Cottonseeds (cotton, cottonseed, Gossypii semen), the seeds of Gossypium nanking Meyen (Mallow and Malvaceae) and related plants, are also widely used as herbal medicines.

Dried rate is a dried chestnut fruit, piled in thorny clusters. It has been used in the shell since it has a deep skin and tastes very good.

The ginger contains about 2% of essential oils, and zingiberene is the main ingredient and also includes zingerone. Used as a spice. Ginger is also used as a medicine to treat gochang (복) and abdominal pain.

In Korea, the record of ginger (1018) appears in Goryeosa, and it is estimated to have been cultivated before that. In Korea, indigestion, dragon, changman, shallow water, vomiting Used for diarrhea, etc.

Health (Zingiberis Rhizoma) consists of Zingerone, Gingerol, Shogaol, Dihydrogingerol, Zingiberol, Cineol, Cinger crab Zingigerone, Borneol and other spicy root stems (根莖). Matches with other medicinal ingredients to boost the effectiveness of diuresis, health, Jinhae, and expectoration

The composition according to the present invention is prepared by the following method using the above-mentioned components.

The lower lobe, the gingiva and the ginger are mixed in the amounts shown in Table 1, and then 5% to 30%, preferably 10% to 20% of 8 to 16 times, preferably 10 to 14 times the total weight of the mixed weight. Mix with weight ethyl alcohol and reflux for 10-40 minutes, preferably 15-30 minutes.

After cooling the mixed solution to room temperature (20 to 25 degrees Celsius), filter the solids in the filter and remove the remaining aqueous solution of the mother liquor (mother liquor) by distillation under reduced pressure to extract the extract in the form of a dark brown concentrate (lower leaves, etc.). X) is generated.

The filtered solid content may be reheated by heating and refluxing the same for the same time again with the same amount of aqueous alcohol solution.

Separately, two to eight times the total weight of the mixed foods, driped sesame, red bean curd, dryness, cottonseed fruit, moth, moth, fruit extract, health, hawthorn, licorice, and seaweed, after mixing Preferably 4 to 6 times 50% to 90%, preferably 60% to 80% by weight of ethyl alcohol, and refluxed for 2 to 8 hours, preferably 4 to 6 hours. .

After cooling the mixed solution to room temperature (20 to 25 degrees Celsius), filter the solids in the filter and remove the remaining aqueous solution of the mother liquor (mother liquor) by distillation under reduced pressure by distillation under reduced pressure. X) is generated.

The filtered solids may be again refluxed by heating and refluxing with the same amount of an aqueous alcohol solution for the same time, followed by filtration, and then mixed with the first extract concentrate.

Mixing the lower lobe and the extract of Euiin and the like produces a final composition (extract).

In the present invention, the dietary fiber, for example, polydextrose, inulin, galacto mannan, chaff, pectin, and the like, in addition to the final extract composition, the hydroxy citric acid (Hydroxy Citric Acid) known for the body fat decomposition effect is the main ingredient Garcinia Cambogia (Garcinia Cambogia) ) May be further mixed, in which case the anti-obesity efficacy of the composition of the present invention may be enhanced.

In addition, the composition prepared by the above method can be prepared in the form of capsules, tablets, slices or tablets by heating, spray drying, freeze drying or vacuum drying. In addition, it can be provided as an oral obesity therapeutic agent containing the composition described above as an active ingredient using this method.

The composition prepared with the composition as described above is easy to procure raw materials using inexpensive herbal ingredients, and exhibits an excellent obesity inhibitory effect.

The present invention also provides a medicament and food containing the herbal composition for inhibiting obesity by providing a method for producing the composition. As a result, to provide a herbal composition that effectively prevents and inhibits obesity and related diseases. In particular, the composition according to the present invention is a mechanism that effectively inhibits α-amylase and lipase, which are carbohydrate digestive enzymes in the body, thereby inhibiting the absorption of carbohydrates and fats in the body to treat obesity and obesity-related diseases, or Shows the effectiveness of prevention

In the embodiment according to the present invention, the composition ratio of the herbal composition mainly composed of the lower lobe extract was prepared as shown in Table 2. This embodiment is described to help the understanding of the present invention, and the content and scope of the present invention are not limited by this embodiment.

Example  One Lower lobe  Preparation of herbal composition mainly composed of extract

Herbal Medicine for Extraction Dosage per person per month (g) Composition ratio (part by weight) Lower leaf (lotus leaf) 1,600 26.1 Cock 400 6.5 ginger 300 4.9 Righteous 900 14.7 Droopy 400 6.5 Small cow 300 4.9 Hamcho 260 4.2 Dry rate 180 2.9 Cotton yarn 260 4.2 A fugitive 400 6.5 Creation 360 5.9 Whiteness 180 2.9 health  40 0.7 Sansa 360 5.9 licorice 180 2.9 sum 6,120 100

Example  1-1. Lower lobe  Preparation of the first extract such as

The lower lobe, gage and ginger are mixed in the amounts shown in Table 2 (lower lobe 1,600 g, gage 400 g and 300 g of ginger), followed by mixing with an aqueous solution of 15% ethyl alcohol at 12 times the total weight of the mixture and heating for 25 minutes. It was refluxed.

After cooling the mixed solution to room temperature (20 to 25 degrees Celsius), the solids are filtered through a filter and the remaining mother liquor is removed by distillation under reduced pressure to remove 282 g of the first extract in the form of a dark brown concentrate (lower leaves, etc.). Got it.

Example 1 -2. Righteous  Preparation of the second extract such as

Separately, the amounts of Euiin, Dried Sew, Red Soybean, Dried Rate, Cottonseed Lobster, Lobster, Creation, Extraction, Health, Sansa, Licorice, Hamcho (Yuiin 900g, Dried Sewage 400g, Reddish Soybean 300g g, cotton wool 260 g, moth 400 g, creation 360 g, baekrye 180 g, health 40 g, hawthorn 360 g, licorice 180 g, seaweed 260 g) after mixing 75% of the total weight It was mixed with an aqueous ethyl alcohol solution and heated to reflux for 4 hours.

After cooling the mixed solution to room temperature (20 to 25 degrees Celsius), the solids were filtered through a filter, and the remaining mother liquor was removed by distillation under reduced pressure to obtain 683 g of a dark brown concentrated liquid as the second extract (Euiin et al.). Got.

Example  1-3. Preparation of the Final Composition

The first extract prepared in Example 1-1, Example 1-2 and the second extract was mixed to prepare a total of 965 g of the final extract to be taken per person 1 month.

Example 2 . In vitro of the final composition ( in in vitro ) α-amylase (α- amylase ) And lipase ( lipase ) Inhibitory effect ( inhibitory activity ) exam

Example 2 -1 α-amylase inhibitory efficacy test

Inhibition of amylase activity was measured using salivary amylase.

50 μL of one unit of amylase enzyme (1 unit frees 1 mg of maltose from starch at 3 ° C. at 20 ° C., pH 6.8) and the concentration of the composition according to the invention in a concentration of 0.04% (400) in 0.2 mol Tris-HCl buffer, respectively ppm) and were incubated at 37 ° C for 5 minutes by adding 0.5 mL of 0.2 mol Tris-HCl buffer.

Then 0.25 mL of 0.5% starch solution was added and incubated at 37 ° C. for 10 minutes.

Then, 0.5 mL of NaOH (8%) solution was added to stop the reaction, 0.5 mL of DNS reagent (1 g of 3,5-dinitrosalicylic acid was dissolved in 20 mL of 2 M sodium hydroxide solution and 30 g of sodium potassium tart) After slowly adding and dissolving sodium potassium tartrate tetrahydrate, the solution was added with distilled water to a final volume of 100 mL) and developed at 100 ° C. for 5 minutes.

After cooling to room temperature, the absorbance was measured at 540 nm with a spectrophotometer to determine the enzyme activity (Conforti et al., Bio. Pharm. Bull., 28, 10981102, 2005).

Table 3 shows the relative values of the amylase activity according to the concentration of the composition according to the present invention with 100 as the absence of the composition, and FIG. 1 shows the amylase activity according to the concentration of the composition according to the present invention. .

Composition concentration Amylase activity 0.00% 100 0.04% 91 0.08% 72 0.12% 61 0.16% 53 0.20% 43

As shown in Table 3 and Figure 1, it can be seen that as the concentration of the composition according to the present invention increases, the activity of amylase, a carbohydrate digestive enzyme, decreases. As a result, the starch digestibility is reduced, which means that the weight is reduced.

Example 2 -2 Lipase ( lipase ) Inhibitory effect ( inhibitory activity ) exam

Lipase activity was measured using 2, 4-dinitrophenylbutyrate as a substrate.

Porcine pancreatic lipase from Sigma, USA (200 units / mL dissolved in 0.1 mol potassium phosphate buffer) was taken and mixed with 0.1 mL of an aqueous solution of the composition according to the present invention.

The composition according to the present invention is 0.04% (400 ppm), 0.08% (800 ppm), 0.12% (1200 ppm), 0.16% (1600 ppm), 0.2% by volume to determine the inhibitory effect of each concentration. (2000 ppm) at 5 concentrations.

0.5 mL of a 0.025 mol 2,4-dinitrophenylbutyrate solution was added to the mixed solution of the lipase solution and the composition solution according to the present invention and reacted at 37 ° C. for 1 minute in an incubator. The activity of the lipase was then determined by measuring the absorbance of 2,4-dinitrophenol liberated by lipase with a spectrophotometer at 360 nm.

Table 4 shows the relative values of the lipase activity according to the concentration of the composition according to the present invention as 100 when there is no composition, and FIG. 2 is a graph showing the lipase activity according to the concentration of the composition according to the present invention. .

Composition concentration Lipase activity 0% 100 0.04% 83 0.08 60 0.12% 45 0.16% 36 0.20% 27

As shown in Table 4 and Figure 2, it can be seen that as the concentration of the composition according to the present invention increases, the activity of the lipase which is a digestive enzyme of fat decreases. As a result, the digestibility of fat is reduced, which means that the weight is reduced.

Example  3. Weight Loss Efficacy Test of Mice by High-fat Diet Using the Compositions According to the Present Invention

In this example, experiments were performed using mouse C57BL / 6 as a test animal, and all of the test subjects were all 10-week-old females for 49 days. The mice were divided into four groups, all of which consisted of five mice, and all conditions other than the feed were kept the same.

-Group 1 (control): 5 groups of oral administration of distilled water (high fat diet, 300 mg / kg body weight orally once a day)

Group 2 (experimental group): 5 oralstat oral administration groups (high-fat feed intake, once daily orallyat 1.6 mg / weight kg oral administration)

-Third group (experimental group): 5 oral administration groups of the composition according to the present invention (high-fat dietary intake, 200 mg / kg body weight once a day)

-Fourth group (experimental group): 5 oral administration groups of the composition according to the present invention (high-fat diet intake, once daily, 400 mg / kg body weight)

At this time, the high-fat diet (Dyets # 101556, manufactured by the Central Experimental Animal Company) is a feed containing 40% (weight ratio) of beef tallow (Baby Tallow) in the basic basic feed (AIN-76A). The feed was not made in the form of pellets because of the high content of fat was provided in the form of dough. Table 5 shows the nutrient content of the high fat diet.

Nutrients Content (%) Calories (kcal / g) protein 17.7 protein 0.732 Fat 40.0 Fat 3.6 fibrin 5.0 carbohydrate 1.210 Ash 4.0 moisture 3.3 carbohydrate 31.4 Total calories 5.542

Table 6 shows the total amount of the body weight (g) of each group of mice fed the high fat diet, and FIG. 3 shows Table 6 graphically.

Elapsed days First group 2nd group 3rd group 4th group 0 days 143 139 140 141 2 days 147.1 139.1 143.1 143.2 7 days 163.4 156.1 152.3 152.5 13th 174.1 164.8 156.4 156.3 17 days 183.2 171.9 159.9 158.7 22 days 192.5 179.3 163 164.7 28 days 201.2 189.9 168.8 165.4 35 days 210.1 202.5 175 172 42 days 220 208.9 183.1 179.8 49 days 230.5 216.8 189.3 185

As shown in Table 6 and FIG. 3, the total weight of the first group that provided water with the feed and the second group which provided the conventional medicine olistat at the same conditions were 230.5 g and 216.8 g, respectively, on the 49th day, The total weight of the third and fourth groups providing the composition according to the invention is 189.3 g and 185 g. This indicates that there is an effect of weight loss when providing the composition according to the present invention with a feed.

Example 4 . Clinical trial of the final composition

In order to test the efficacy of the composition according to the present invention for obese women, extracts were prepared by the method described in Example 1 using the amounts of the herbal preparations as shown in Table 2, and administered for 2 months, and the body weight, body fat and BMI before and after administration. Changes in the index were considered. At this time, the body fat analyzer was used a voluntary medical body fat analyzer (model name BIA-530).

Table 7 shows the clinical data of the clinical participants before administration of the composition according to the present invention, and Table 8 shows the clinical data of the clinical participants 2 months after the administration of the composition according to the present invention.

Table 9 shows a comparison of the clinical efficacy test results of the clinical participants before and after administering the composition according to the present invention, Figure 4 shows the test results in Table 9 graphically.

Clinical data of clinical participants prior to administration of the composition according to the present invention gender age Weight (kg) Height (cm) % Of body fat Muscle mass (kg) Visceral Fat Level BMI Index female 28 63.8 158 33 39 9 25.6 female 41 76.8 160 37 44 13 30 female 54 57.5 156 32 35.9 10 23.6 female 39 55.3 154 31.4 34.8 9 23.3 female 36 58.4 150 35.6 34.3 12 26 female 41 56.2 153 31.8 35.1 9 24 female 30 60.4 159 31.8 37.8 9 23.9 female 33 58.7 163 31.5 36.8 9 22.1 female 36 80.3 164 37.1 46 13 29.9 female 39 56 160 29.4 36.3 7 21.9 female 33 56.3 158 29.1 36.7 7 22.6 female 32 75.5 163 33.9 45.6 10 28.4 female 45 79.8 162 37.2 45.6 14 30.4 female 35 78.6 160 37.8 44.5 13 30.7 female 37 61.9 158 28.6 40.6 7 24.8 female 34 91.4 164 39.1 50.4 14 34 female 28 68.6 170 29.4 44.4 7 23.7 female 51 65.4 158 33 40.1 11 26.2 female 53 67.6 157 33.4 41.2 11 27.4 female 42 60.3 160 30.8 38.2 9 23.6 female 46 67.7 165 30.4 43.2 9 24.9 female 47 61.2 159 30.2 39.2 9 24.2 female 45 59.3 159 34.2 35.7 12 23.5 female 49 78.7 153 42.3 41 17 33.6 female 43 70.7 163 32.9 43.4 10 26.6 female 55 51.7 151 33.1 31.7 11 22.7 female 28 66.1 160 32.8 40.7 9 25.8 female 53 68.4 158 35.7 40.2 13 27.4 female 47 60.8 158 37.8 34.4 14 24.4 female 40 72.9 159.5 35.5 42.8 12 28.7 female 30 62.4 165 27.4 41.7 6 22.9 south 50 65.8 163 25.1 45.5 13 24.8 south 40 88 180 24.9 61 12 27.1 south 54 75.1 170 25.2 51.9 13 26 Average 41.0 66.99 160.3 32.7 41.2 10.7 26.0

Clinical data two months after administration of the patent composition gender age Weight (kg) Height (cm) % Of body fat Muscle mass (kg) Visceral Fat Level BMI Index female 28 59.5 158 30.9 31.9 8 23.8 female 41 69.5 160 32.6 41 11 27.1 female 54 57 156 32 35.7 10 23.4 female 39 54 154 30.5 33,7 8 22.8 female 36 54.3 150 32.7 34 11 24.1 female 41 54 153 30.1 34.1 9 23.1 female 30 55.4 159 29.8 36 8 21.9 female 33 53.9 163 29.8 35.3 8 20.3 female 36 71.4 164 31.4 43 11 26.5 female 39 55.5 160 29 35.8 7 21.7 female 33 56.2 158 29 36.7 7 22.5 female 32 70.3 163 31.1 43.8 9 26.6 female 45 68.5 162 32.1 42.2 12 26.1 female 35 69.3 160 31.3 41.7 11 27.1 female 37 59.5 158 27.2 39.6 7 23.8 female 34 79.5 164 33.5 46.7 12 29.6 female 28 66.6 170 28 43 7 23 female 51 60.2 158 30.1 37.8 10 24.1 female 53 60.6 157 28.6 38.4 10 24.6 female 42 55.4 160 28.3 37.2 8 21.6 female 46 62.7 165 29.5 42.1 8 23 female 47 58.8 159 29 37 8 23.3 female 45 52.7 159 32.1 33.8 11 20.8 female 49 68.5 153 37.1 39 14 29.3 female 43 63.6 163 30.1 41 9 23.9 female 55 51.9 151 33.3 32 11 22.7 female 28 62.1 160 30.3 39.5 8 24.3 female 53 61.7 158 32.7 38.2 11 24.7 female 47 54.4 158 31.1 30.5 12 21.8 female 40 69.5 159.5 34.1 41.9 11 27.3 female 30 62.1 165 27.2 41 6 22.8 south 50 62.8 163 23.9 44.6 12 23.6 south 40 81.2 180 20.7 58 11 25.1 south 54 72.3 170 24 50.2 12 25 Average 41 62.20 160.3 30.1 39.8 9.65 24.2

Composition Administration Clinical Efficacy Test Results Before dosing (mean) After dosing (average) weight 66.99 (kg) 62.2 (kg) Body fat percentage 32.7 (%) 30.1 (%) Muscle mass 41.2 (kg) 39.8 (kg) Visceral Fat Level 10.7 9.65 BMI Index 26.0 24.2

As shown in Tables 7 to 9 and FIG. 4, the results of clinical trials of the composition according to the present invention for two months showed that the body weight was reduced from 66.99 kg to 62.2 kg, and the body fat percentage was also reduced by 30.1% to 30.1%. It is showing weight loss effect. In addition, visceral fat level and BMI index also decreased from 10.7 and 26.0 to 9.65 and 24.2, respectively.

1 is a graph showing amylase activity according to the concentration of the composition according to the present invention.

Figure 2 is a graph showing the lipase activity according to the concentration of the composition according to the present invention.

Figure 3 is a graph showing the total content of the weight of each group of mice fed a high-fat diet according to the invention and the present invention.

Figure 4 is a graph showing the comparison of the clinical efficacy test results of the clinical participants before and after administering the composition according to the present invention.

Claims (7)

Lower lobe 140 to 180 parts by weight, gage 30 to 50 parts by weight, 20 to 40 parts by weight ginger, 80 to 100 parts by weight of Euiin, 30 to 50 parts by weight, red beans 20 to 40 parts by weight, 20 to 30 parts by weight of seaweed, 15 to 25 parts by weight of dry weight, 10 to 30 parts by weight of cotton yarn, 30 to 50 parts by weight, moth 30 to 40 parts by weight, 15 to 25 parts by weight, 1 to 7 parts by weight of health, 30 to 40 parts by weight of hawthorn and A composition for inhibiting obesity and treating obesity consisting of an extract of 15 to 25 parts by weight of licorice extracted with alcohol. The method of claim 1, Obesity inhibition and obesity treatment composition further comprising a dietary fiber. The method of claim 1, A composition for inhibiting obesity and treating obesity, further comprising garcinia cambogia. After mixing the lower lobe, cage and ginger, the mixture is mixed with 8 to 16 times 5% to 30% by weight of ethyl alcohol and heated to reflux for 10 to 40 minutes, and the mixed solution is cooled to room temperature, and then the solid content is Filtering and distilling the remaining aqueous solution of alcohol in a reduced pressure to prepare a first extract; Euiin, Dried Sewage, Red Soybean, Dry Rate, Cottonseed Lobster, Lobster, Creation, Extraction, Health, Sansa, Licorice, Hamcho Heating to reflux for 2 to 8 hours, cooling the mixed solution to room temperature, filtering the solids, and distilling under reduced pressure the aqueous alcohol solution of the remaining mother liquor to prepare a second extract; Method for producing a composition for inhibiting obesity and obesity treatment comprising the step of mixing the first extract and the second extract. The method of claim 4, wherein Method of producing a composition for inhibiting obesity and treating obesity further comprises the step of mixing the filtered solid content with an aqueous solution of alcohol and refluxed and then mixed with the first extract and the second extract, respectively. A method for preparing an anti-obesity agent, characterized in that the composition for inhibiting obesity and treating obesity prepared by claim 4 is prepared in the form of capsules, tablets, slices or tablets by heating, spray drying, lyophilization or vacuum drying. An oral obesity treatment agent comprising the composition for inhibiting obesity and treating obesity according to claim 1 as an active ingredient.

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