KR20080101148A - The manufaturing process of plant growth promoter composition using fermetation productions of effective microorganisms - Google Patents

Abstract

A method of manufacturing a composition for promoting growth of plant is provided to increase a chlorophyll in a plant body using nature fermentation product of useful microorganism such as lactobacillus, photosynthetic bacteria, yeast and Bacillus, to make color of stem and leaf deep, to accelerate growth of the leaf and root, to promote coloring of fruit and to prevent failure of the growth of plant. A method of manufacturing a composition for promoting growth of plant comprises steps of: obtaining many fermentation products 1 containing a material promoting growth of plant by cultivating independently photosynthetic bacteria which is a useful microorganism in a fermentation medium; obtaining a fermentation product 2 in which many bioactive substances, enzymes, antioxidants, organic acids are contained by forming a symbiotic relation by mixing and inoculating various useful microorganism such as lactobacillus, photosynthetic bacteria, yeast and Bacillus to the other fermentation medium; and mixing the fermentation product 1 and the fermentation product 2 at a regular rate.

Description

The manufaturing process of plant growth promoter composition using fermetation productions of effective microorganisms}

1 is a graph comparing the chlorophyll content of radish according to the treatment method for plant growth promoting composition of the present invention.

Figure 2 is a graph analyzing the various organic acids contained in the plant growth promoting composition of the present invention by HPLC.

Figure 3 is a photograph showing a comparison of the growth of radish according to the treatment method for plant growth promotion of the present invention.

The present invention relates to a method for preparing a composition for promoting plant growth, and more specifically, to obtain a fermentation product 1 containing a large amount of plant growth promoting material by culturing photosynthetic bacteria, which is useful for fermentation medium of a certain composition, alone. By inoculating mixed fermentation medium with various useful microorganisms such as lactic acid bacteria, photosynthetic bacteria, yeast, and Bacillus to form a symbiotic relationship, obtain fermentation products 2 containing a large amount of natural physiologically active substances, enzymes, antioxidants, and organic acids. It relates to a method for producing a composition for promoting plant growth by mixing.

Plant growth promoters that are conventionally commercialized may be produced when a plant hormone is produced through a series of manufacturing processes, such as chemical synthesis, and directly commercialized a specific microorganism having a characteristic of secreting plant hormones. Phytohormones are organic substances that are not nutrients and cause specific physiological, biochemical or morphological reactions at specific concentrations. Currently, commercially available plant growth promoters are gibberellin, auxin and cytokinin. There are various substances such as (cytokinin), ABA, and ethylene, and 25 kinds of substances including the above are reported in the plant growth regulators in Korea. However, phytohormones may have disadvantages that affect only specific crops, not various crops, and it is difficult to expect high quality and high yield due to abnormal enlargement of crops if the treatment concentration, application time, and application method are different. There is a case. In addition, most of the plant hormones are expensive as imports, and since they are not soluble in water other than gibberellin when preparing the solution, alcohol must be used as a dissolving agent, so that alcohol penetrates into the plant and generates heat, causing the plant to wither.

As one of the methods for improving this, Korean Unexamined Patent Publication No. 10-2007-0015528 (5-aminolevulinate, a preparation method thereof and use thereof), 10-2006-0016685 (deltaaminolevulin as a high-functional crop growth regulator and herbicide Novel Use of Acids) and the Ministry of Agriculture Research Report (determining the mechanism of action of δ-Aminolevulinic acid (ALA) and the development of biopesticides) using 5-aminolevulinic acid or 5-aminolevulinate produced by recombinant strains. Although the growth promoting effect has been revealed for various crops, fermented products produced by genetically modified strains are difficult to contain various natural bioactive substances that can act as plant growth promoting factors in addition to 5-aminolevulinic acid. In addition, the manufacturing cost is high, and when produced using the material produced by the recombinant strain, there was a limit that may adversely affect the image of agricultural products All.

Accordingly, an object of the present invention is to provide a method for preparing a plant growth promoting composition, which is safe and simple to use, and economical using natural fermentation products by useful microorganisms such as lactic acid bacteria, photosynthetic bacteria, yeast, and Bacillus.

In order to achieve the object of the present invention,

1) Prepare fermentation medium containing about 0.1 ~ 2.0% by weight of carbon nutrient source, 0.1 ~ 1.0% by weight of nitrogen nutrient source, 0.01 ~ 0.2% by weight of phosphate nutrient source, and 0.1 ~ 2.0% by weight of other nutrient sources and dissolve in groundwater. Inoculate photosynthetic bacteria and incubate for 3 to 5 days while maintaining 20 ~ 35 ℃ to obtain a fermentation product 1 containing 5-aminolevulinic acid known as plant growth promoting material. Table 1 summarizes the analysis method for 5-aminolevulinic acid.

TABLE 1

5- Aminolevulinic acid  analysis method 1. Add 2.8 ml of acetic acid buffer (pH 4.6) and 0.2 ml of acetylacetone to 7 ml of the culture supernatant, heat and cool at 100 ° C for 15 minutes, and take 2 ml. 2. Modified Ehrlich reagent in 2 ml. After the reaction was conducted for 15 minutes at room temperature, the absorbance was measured at 553 nm. acetic acid buffer (pH 4.6): After adding 136 g of sodium acetate trihydrate to 57 ml of glacial acetic acid, D.W. Fit 1L. I. modified Ehrlich reagent: Dissolve 1 g of DMAB (ρ-dimethylaminobenzaldehyde) in 42 ml of glacial acetic acid and 8 ml of 70% perchloric acid.

2) Prepare fermentation medium containing 1 ~ 20% by weight of carbon nutrient source, 0.1 ~ 1% by weight of nitrogen nutrient source, 0.01 ~ 0.2% by weight of phosphate nutrient source, dissolve in groundwater, and then dissolve in lactic acid bacteria, photosynthetic bacteria, yeast, and Bacillus By inoculating the bacteria and incubated for 5 to 20 days while maintaining 20 ~ 35 ℃ to obtain a fermentation product 2 containing a large amount of bioactive substances such as organic acids.

3) It provides a method for producing a plant growth promoting composition by mixing the fermentation products 1 and 2) fermentation products 2 of 1) in an appropriate ratio.

Hereinafter, the present invention will be described in detail.

Among the fermentation broths, components that can be used as a carbon nutrient source include glucose, fructose, maltose, molasses, and lactose. The components that can be used as a nitrogen nutrient source are peptone, tryptone, urea, yeast extract, malt extract, beef extract, and the like. The phosphate nutrients can be used as potassium phosphate, sodium phosphate and the like.

Photosynthetic bacteria of the inoculated microorganism of the present invention can be used Rhodo Pseudomonas bacteria, Rhodobacter bacteria, Rhodophyllium bacteria, Lactobacillus bacteria, Lactobacillus bacteria, Pediococcus bacteria can be used have.

Culture conditions of step 1) is characterized in that the ORP is maintained at -70 ~ 0mv by maintaining the aeration amount to 0.1 ~ 0.5vvm.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited only to these examples.

Example  Useful microorganisms Fermented products  Production of plant growth promoting composition using

Groundwater and glucose 1.0% by weight, yeast extract 0.5% by weight, potassium monophosphate 0.05% by weight, sodium diphosphate 0.2% by weight, sodium glutamate 0.5% by weight, malic acid 0.5% by weight, ammonium sulfate 0.3% by weight, magnesium sulfate 0.05% by weight After dissolving the fermentation broth together with%, inoculated with Rhodoshudomonas bacteria to maintain 30 ~ 35 ℃ and to control ORP to maintain -70 ~ 0mv by incubating for 4 days while controlling the plant growth material Fermentation product 1 containing 5-aminolevulinic acid was obtained. As a result of measuring 5-aminolevulinic acid by the method according to Table 1, it was confirmed that 1g / L was contained. In another fermentation tank, ground water and molasses, 10% by weight of yeast extract, and 1% by weight of fructose were added together to dissolve, followed by dissolution of one strain of Lactobacillus, one species of genus Lukonostoc, and one of Rhodobacter. Fermented product containing a large amount of excellent bioactive substances, enzymes, antioxidants and organic acids by inoculating 1 species, 1 species of Saccharomyces and 1 species of Bacillus, and incubating for 10 days while maintaining at 30-35 ℃ 2 was obtained (see Fig. 2). Each fermentation product was then mixed at 50:50 to prepare a composition for promoting plant growth.

Experimental Example  1: effect on the growth of young radish

Prepare three 75cm * 40cm * 45cm garden pots in a plastic house, mix gardening clay [Toby Tech Co., Ltd .: 'Merrit'] and Masato 1: 1 and fill each pot equally, and then heat radish more than 500 Sowing was soy. At one week intervals, one pot was sprayed with only groundwater as a control while maintaining a temperature of 20 to 30 ° C. Ports 1 and 2, respectively, were sprayed with 5,000-fold dilutions and 10,000-fold dilutions of the plant growth-promoting compositions produced by the examples. Sprayed. Confirmation of plant growth promoting effect after 21 days was summarized in Table 2.

TABLE 2

Control (no treatment) 10,000-fold dilution 5,000-fold dilution Leaf length (cm) 5.2 7.8 7.6 Leaf width (cm) 2.7 3.4 3.5 Leaves () 3.1 3.4 3.2 Ground weight (g) 10.3 12.4 12.7 Root Length (cm) 5.9 6.3 6.3 Underground gross weight (g) 4.5 6.0 6.25

From the results of Table 2 it was confirmed that the composition for promoting plant growth of the present invention promotes the growth of radish leaves and roots. In addition, as shown in Figure 1 it can be seen that the heat chlorophyll content of 10,000 times treatment and 5,000 times treatment compared to the control.

Experimental Example  2: Effect on apple yield and hypertrophy / coloring

Two out of four five-year-old apple trees [variety "Fuji"] with the same conditions were selected as controls and treatments. The treatment area was sprayed with 10,000-fold dilution of the plant growth promoting composition produced by the example five times, once every three weeks, from full bloom in April to July, and the control area was sprayed with the same amount of groundwater. It was. After harvesting, the coloring status of the fruit, the total amount of fruit, the weight, etc. were compared and summarized in Table 3.

TABLE 3

 Total yield ()  Total weight (kg) Coloring degree () 60% or more coloring ratio 50 ~ 59% coloring ratio Coloration ratio less than 50%  Control A 185 46.25 32 87 66 A ' 190 49.40 34 90 66  Treatment B 210 55.65 52 95 63 B ' 200 55.00 51 92 57

 * Coloration ratio is the ratio of the colored area to the total area.

From the results of Table 3, it was confirmed that the composition for promoting plant growth of the present invention showed an increase in yield, coloring and hypertrophy of apples.

Experimental Example  3: effect on the growth and yield of cucumbers

One month after transplanting the seedlings (named "Tadada"), which had been transplanted to the plastic house, were divided into control and treatment, and once every 10 days, 10,000-fold dilutions of the plant growth-promoting composition produced by the example were foliarly sprayed. The control area was sprayed with the same amount of groundwater. The yield of 15 days was investigated and summarized in Table 4.

TABLE 4

Control ( Non-treated ) (Unit: pieces) Treatment Unit (Unit: unit) Day 1 45 46 Day 2 44 51 Day 3 42 52 Day 4 45 51 Day 5 40 53 Date 6 31 47 Date 7 25 47 Date 8 38 50 Date 9 38 50 Date 10 40 52 Date 11 40 52 Date 12 45 56 Date 13 44 56 Date 14 44 54 Date 15 41 56 Sum 602 722

 * Only fruit that is commercially available is counted as the yield.

From the results of Table 4 it was confirmed that the composition for promoting plant growth of the present invention has the effect of promoting the growth and yield of cucumber.

Experimental Example  4: effect on salting of tomatoes

Two horticultural pots were prepared, mixed with horticultural soil [Toby Tech Co., Ltd .: 'Merit'] and Masato 1: 1, and filled in each pot with the same weight, and 10 seedlings of tomato seedlings were transplanted. One of the two pots was sprayed once a week with foliar spray of a 5,000-fold dilution of the plant growth promoting composition produced in Example as a treatment, and the control pot was grown in a greenhouse for 7 weeks while spraying the same amount of groundwater. At this point, after 15 weeks, 15 of 20 port bottom drainage holes of control and treatment ports were blocked, and sodium chloride was dissolved in water in an amount equivalent to 1.5% by weight of soil in each port. The degree of salting of the tomatoes was summarized in Table 5 by evaluating in six steps described below.

Evaluation stage

   0: No salt is seen at all.

   1: A very weak salt is seen overall.

   2: A weak salt is seen in less than 3 aeration.

   3: Over 5 aerations, apparent salting is seen.

   4: Strong salting is seen above 7 aeration.

   5: More than 5 aerations are killed by salting.

TABLE 5

Control ( No treatment ) Treatment Day 1 Sodium Chloride Treatment 0 0 Day 3 of Sodium Chloride Treatment One 0 Day 5 of sodium chloride treatment One 0 Week 7 of Sodium Chloride Treatment 2 One Day 9 of Sodium Chloride Treatment 2 One Day 11 of sodium chloride treatment 3 One Week 13 of Sodium Chloride Treatment 3 2 Day 15 of sodium chloride treatment 4 2 Day 17 of sodium chloride treatment 4 2 Day 19 of sodium chloride treatment 5 2 Day 21 of Sodium Chloride Treatment 5 3

From the results in Table 5, it was confirmed that the composition for promoting plant growth of the present invention improved resistance to salting of tomatoes.

Experimental Example  5: effect on the coldening of tomatoes

Three horticultural pots were prepared, mixed with horticultural soil [Toby Tech Co., Ltd .: 'merit'] and Masato in a 1: 1 weight and filled in each pot, and then 10 tomato seedlings were transplanted. One of the two pots was sprayed once a week with foliar spray of a 5,000-fold dilution of the plant growth promoting composition produced in Example as a treatment and the control pot was grown in a greenhouse for 4 weeks while spraying the same amount of groundwater. And the control and treatment were moved to a low temperature (4 ℃) condition and evaluated the degree of cold thaw of 4 days in six steps described below summarized in Table 6.

Evaluation stage

   0: There is no cold at all.

   1: Very mild cold overall.

   2: A mild cold sea is seen below 3 aeration.

   3: Over 5 aerations, apparent cold water is seen.

   4: Strong cold sea is seen at 7 or more wastes.

   5: Five or more air bubbles die by cold sea.

TABLE 6

Control ( No treatment ) Treatment Day 1 of Low Temperature 2 One Day 2 of cold conditions 3 2 Day 3 of cold conditions 5 2 Day 4 of low temperature condition 5 3

From the results of Table 6, it was confirmed that the composition for promoting plant growth of the present invention improved resistance to cold damage of tomatoes.

As shown in the above examples and experimental examples, the composition for promoting plant growth according to the present invention increases the chlorophyll in the plant when sprayed on the leaves and stems of the plant to increase the color of the leaves and stems, and to increase the growth of the roots and leaves. It has the effect of promoting the coloring and enlargement of fruits, and preventing plant growth disorders under poor conditions such as low sunshine, low temperature, and salt accumulation. In particular, the increase in chlorophyll plays a role in improving the photosynthetic capacity of the plant, in addition to the above effects can be expected a variety of growth promoting effect.

Claims (3)

By cultivating the photosynthetic bacteria, which are useful microorganisms in a certain composition, fermentation product 1 containing a large amount of plant growth-promoting substances is obtained. Method for producing a composition for promoting plant growth, characterized in that to obtain a fermentation product 2 containing a large amount of natural physiologically active substances, enzymes, antioxidants, organic acids by mixing to form a relationship. The method of claim 1, The inoculated microorganism used in the fermentation product 1 culture is selected from Rhodoschudomonas bacteria, Rhodobacterium bacteria, and Rhodophyllium bacteria, and the culture condition is ORP is -70 by maintaining aeration rate of 0.1 ~ 0.5vvm A method for producing a composition for promoting plant growth, characterized by containing 0.5 to 10 g / L of 5-aminolevulinic acid by culturing to ˜0 mv. The method of claim 1, Among the inoculated microorganisms used in the fermentation product 2, photosynthetic bacteria are one of the genus Rhodoschudomonas, Rhodobacter, and Rhodophyllium, and the lactic acid bacteria are Lactobacillus, Luconostok, and Pdiococcus. Method for producing a composition for promoting plant growth, characterized in that the culture of two selected from.

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