KR20080099636A - 유해 활성산소 소거효과가 있는 참당귀 추출물의 추출방법 - Google Patents
유해 활성산소 소거효과가 있는 참당귀 추출물의 추출방법 Download PDFInfo
- Publication number
- KR20080099636A KR20080099636A KR1020070045441A KR20070045441A KR20080099636A KR 20080099636 A KR20080099636 A KR 20080099636A KR 1020070045441 A KR1020070045441 A KR 1020070045441A KR 20070045441 A KR20070045441 A KR 20070045441A KR 20080099636 A KR20080099636 A KR 20080099636A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- ethanol
- angelica
- supernatant
- concentrate
- Prior art date
Links
- 240000001810 Angelica gigas Species 0.000 title claims abstract description 29
- 235000018865 Angelica gigas Nutrition 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 20
- 150000003254 radicals Chemical class 0.000 title abstract description 10
- 230000000694 effects Effects 0.000 title description 3
- 230000002000 scavenging effect Effects 0.000 title description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 83
- 239000000284 extract Substances 0.000 claims abstract description 45
- 239000012141 concentrate Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000002904 solvent Substances 0.000 claims abstract description 5
- 239000006228 supernatant Substances 0.000 claims description 12
- 241000125175 Angelica Species 0.000 claims description 9
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 9
- 230000007760 free radical scavenging Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000011346 highly viscous material Substances 0.000 claims description 2
- 238000001704 evaporation Methods 0.000 claims 4
- 238000001035 drying Methods 0.000 claims 3
- 239000012535 impurity Substances 0.000 claims 2
- 230000003381 solubilizing effect Effects 0.000 claims 2
- 239000002994 raw material Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 7
- CUKSFECWKQBVED-INIZCTEOSA-N Decursin Chemical compound C1=CC(=O)OC2=C1C=C1C[C@H](OC(=O)C=C(C)C)C(C)(C)OC1=C2 CUKSFECWKQBVED-INIZCTEOSA-N 0.000 abstract description 6
- CUKSFECWKQBVED-UHFFFAOYSA-N Grandivittin Natural products C1=CC(=O)OC2=C1C=C1CC(OC(=O)C=C(C)C)C(C)(C)OC1=C2 CUKSFECWKQBVED-UHFFFAOYSA-N 0.000 abstract description 6
- JXZWWIMXTVJNSF-UHFFFAOYSA-N decursin Natural products CC(=CC(=O)OC1Oc2cc3OC(=O)C=Cc3cc2CC1(C)C)C JXZWWIMXTVJNSF-UHFFFAOYSA-N 0.000 abstract description 6
- AGABNGOXUSXQDD-XKGFZTIGSA-N [(3s)-2,2-dimethyl-8-oxo-3,4-dihydropyrano[3,2-g]chromen-3-yl] (z)-2-methylbut-2-enoate Chemical compound C1=CC(=O)OC2=C1C=C1C[C@H](OC(=O)C(\C)=C/C)C(C)(C)OC1=C2 AGABNGOXUSXQDD-XKGFZTIGSA-N 0.000 abstract description 5
- AGABNGOXUSXQDD-UHFFFAOYSA-N decursinol angelate Natural products C1=CC(=O)OC2=C1C=C1CC(OC(=O)C(C)=CC)C(C)(C)OC1=C2 AGABNGOXUSXQDD-UHFFFAOYSA-N 0.000 abstract description 5
- BGXFQDFSVDZUIW-UHFFFAOYSA-N Decursinol Natural products O1C(=O)C=CC2=C1C=C1OC(C)(C)C(O)CC1=C2 BGXFQDFSVDZUIW-UHFFFAOYSA-N 0.000 abstract description 4
- BGXFQDFSVDZUIW-LBPRGKRZSA-N decursinol Chemical compound O1C(=O)C=CC2=C1C=C1OC(C)(C)[C@@H](O)CC1=C2 BGXFQDFSVDZUIW-LBPRGKRZSA-N 0.000 abstract description 4
- 230000003078 antioxidant effect Effects 0.000 description 13
- 239000003814 drug Substances 0.000 description 6
- 239000003963 antioxidant agent Substances 0.000 description 5
- 235000006708 antioxidants Nutrition 0.000 description 5
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 238000000746 purification Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 229920002567 Chondroitin Polymers 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical compound CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 description 2
- 231100001261 hazardous Toxicity 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000401 methanolic extract Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 230000008836 DNA modification Effects 0.000 description 1
- 208000002249 Diabetes Complications Diseases 0.000 description 1
- 206010012655 Diabetic complications Diseases 0.000 description 1
- MDNQVRKYHGPJNA-UHFFFAOYSA-N Gigasol Natural products CC(C)(O)C(O)Cc1cc2C=CC(=O)Oc2c(COCc3c(O)c(CC(O)C(C)(C)O)cc4C=CC(=O)Oc34)c1O MDNQVRKYHGPJNA-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 240000006794 Volvariella volvacea Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000006851 antioxidant defense Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000417 nephrotoxicity Toxicity 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000013014 purified material Substances 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 229940125723 sedative agent Drugs 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/02—Antioxidant
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (7)
- 에탄올과 물을 용매로 사용하고 온도와 용해도 차이를 이용하여 데커신, 데커시놀, 데커시놀 안젤레이트를 주성분으로 하는 참당귀 추출농축물의 생산방법
- 제 1항에 있어서, 상기 참당귀 추출농축물이란 데커신, 데커시놀 안젤레이트, 데커시놀을 순도 90% 이상 함유하는 것을 특징으로 하는 참당귀 추출농축물의 생산방법
- 제2항에 있어서, 상기 참당귀 추출농축물이란 데커신, 데커시놀 안젤레이트, 데커시놀을 순도 95% 이상 함유하는 것을 특징으로 하는 참당귀 추출농축물의 생산방법
- 제 1항 내지 제3항 중 어느 한 항에 있어서, 상기 참당귀 추출농축물은 유해 활성산소(free radical) 소거 작용을 가지는 것을 특징으로 하는 참당귀 추출농축물의 생산방법
- 제4항에 있어서, 상기 참당귀 추출농축물은 유해 활성산소(free radical) 소거작용 50%(SC50)이 0.047중량%를 나타내는 것을 특징으로 하는 참당귀 추출농축물 의 생산방법
- 제 1항에 있어서, 상기 참당귀 추출농축물의 생산방법은원료인 참당귀의 2배에서 4배의 에탄올을 첨가하여 진탕하에서 추출하고 농축하여 점성이 높은 물질을 얻는 단계 ;이 물질을 에탄올로 주성분을 녹이고 영하의 온도에서 방치하여 생성된 침전물을 제거하여 상등액을 얻고 상등액을 증발건고하여 농축물을 얻는 단계 ;이 농축물에 주성분의 용해도를 이용하여 물로 희석된 에탄올을 사용하여 가용화 시키는 단계 ;희석 에탄올에 녹지 않는 불순물을 원심분리하여 제거하는 단계 ;희석 에탄올층을 취하여 증발 건고하는 단계:증발건고된 물질을 에탄올에 녹여 상등액을 취하는 단계 :이 상등액을 재 증발건고하여 주성분인 데커신과 데커시놀 안젤레이트 혼합물을 얻는 단계를 포함하는 것을 특징으로 하는 참당귀 추출농축물을 생산하는 방법
- 제 1항에 있어서, 상기 참당귀 추출농축물의 생산방법은,원료인 참당귀를 40메시(mesh)이하로 잘게 분쇄하여 수분함량 5%이하로 건조하고 분쇄한 참당귀에 분쇄 참당귀의 2배에서 4배의 에탄올을 첨가하여 진탕하에서 추출하고 농축하여 점성이 높은 물질을 얻는 단계 ;이 물질 1 킬로그람당 에탄올 1리터를 넣어 주성분을 녹이고 영하 20도에서 10시간 방치하여 생성된 침전물을 원심분리하여 제거한 후 상등액을 얻고 상등액을 증발건고하여 농축물을 얻는 단계 ;이 농축물에 주성분의 용해도를 이용하여 물로 희석된 에탄올(60% 에탄올)을 사용하여 가용화 시키는 단계 ;희석 에탄올(60% 에탄올)에 녹지 않는 불순물을 원심분리하여 제거하는 단계 ;희석 에탄올층을 취하여 증발 건고하는 단계:증발건고된 물질을 99% 에탄올에 녹여 상등액을 취하는 단계 :이 상등액을 재 증발건고하여 주성분인 데커신과 데커시놀 안젤레이트 혼합물을 얻는 단계를 포함하는 것을 특징으로 하는 참당귀 추출농축물을 생산하는 방법
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020070045441A KR100893779B1 (ko) | 2007-05-10 | 2007-05-10 | 유해 활성산소 소거효과가 있는 참당귀 추출물의 추출방법 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020070045441A KR100893779B1 (ko) | 2007-05-10 | 2007-05-10 | 유해 활성산소 소거효과가 있는 참당귀 추출물의 추출방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20080099636A true KR20080099636A (ko) | 2008-11-13 |
KR100893779B1 KR100893779B1 (ko) | 2009-04-20 |
Family
ID=40286577
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020070045441A KR100893779B1 (ko) | 2007-05-10 | 2007-05-10 | 유해 활성산소 소거효과가 있는 참당귀 추출물의 추출방법 |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR100893779B1 (ko) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101233967B1 (ko) * | 2010-11-26 | 2013-02-18 | 경성대학교 산학협력단 | 데커신 및/또는 데커시놀 안젤레이트를 유효성분으로 함유하는 막걸리 및 그 제조방법 |
KR101658350B1 (ko) | 2015-02-06 | 2016-09-30 | 경성대학교 산학협력단 | 고지혈증 치료에 유용한 약학 조성물 |
KR20210150829A (ko) | 2020-06-04 | 2021-12-13 | 경성대학교 산학협력단 | 참당귀 추출물 및 인디루빈 농축물을 포함하는 건선 또는 비염치료용 조성물 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69917796T2 (de) * | 1998-10-22 | 2005-06-09 | Chong, Se Young | Decursin-enthaltende pharmazeutische zusammensetzungen |
KR100509843B1 (ko) * | 2003-03-14 | 2005-08-24 | 주식회사 바이넥스 | 참당귀로부터 데커신 및 데커시놀 안젤레이트를 추출하는 방법 |
KR100633096B1 (ko) * | 2005-08-27 | 2006-10-12 | 김형환 | 데커신 또는 데커시놀 안젤레이트를 함유하는 혈관이완제 |
-
2007
- 2007-05-10 KR KR1020070045441A patent/KR100893779B1/ko active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
KR100893779B1 (ko) | 2009-04-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Simões et al. | Effect of Brazilian green propolis on the production of reactive oxygen species by stimulated neutrophils | |
AU2012338742B2 (en) | Composition comprising chicory extract | |
RU2322091C1 (ru) | Композиция биологически активных веществ и способ получения нанодисперсии ее | |
RU2432169C2 (ru) | Фосфолипидные комплексы из экстрактов листьев или плодов маслины, обладающие улучшенной биодоступностью | |
KR100509843B1 (ko) | 참당귀로부터 데커신 및 데커시놀 안젤레이트를 추출하는 방법 | |
CN111249338A (zh) | 一种苁蓉提取物及其工业化制备方法和用途 | |
KR100893779B1 (ko) | 유해 활성산소 소거효과가 있는 참당귀 추출물의 추출방법 | |
Riasat et al. | Scrutinizing the therapeutic response of Phyllanthus exmblica's different doses to restore the immunomodulation potential in immunosuppressed female albino rats. | |
KR100414187B1 (ko) | 흰점박이꽃무지 추출물 및 이의 용도 | |
Campuzano-Bublitz et al. | Acute and chronic anti-hyperglycemic effect of Prosopis ruscifolia extract in normoglycemic and alloxan-induced hyperglycemic rats | |
KR101144715B1 (ko) | 정자수 증가용 경구 투여제 | |
Ziaul Amin et al. | Evaluation of medicinal effects of Gynuraprocumbens leave extracts on oxidative, glycemic, lipidomics, and enzymatic profiles in alloxan-induced diabetic mice | |
US10137159B2 (en) | Boehmeria extract and its use in treating liver diseases | |
CN107441129B (zh) | 金花葵核苷类物质的发现及其制备方法与应用 | |
TWI618540B (zh) | Composition for preventing renal toxicity caused by drug toxicity, preparation method thereof and Its use | |
RU2665968C1 (ru) | Способ получения средства, обладающего желчегонной, противовоспалительной и антиоксидантной активностью | |
JPS627171B2 (ko) | ||
RU2404789C2 (ru) | Способ получения биологически активного экстракта из корня молочая палласа | |
Aldhafiri et al. | Hepatoprotective studies of methanolic leaf extract of Barringtonia acutangula (L) against carbon tetrachloride induced liver injury in wistar rats | |
BG97371A (bg) | Антинеопластичен хемотерапевтик от растителен произход, притежаващ висока селективност и силно понижена токсичност и метод за неговото получаване | |
Rahman et al. | Anti-Inflammatory and Analgesic Activities of Ethanolic Extract of Glochidion Thomsonii (BARK) | |
RU2797119C2 (ru) | Способ получения средства, обладающего иммуномодулирующей активностью | |
Alfarajat et al. | Reno-protective effect of Citrus sinensis by regulating antioxidant capability and gene expression in adenine-induced chronic kidney in rats | |
RU2223110C1 (ru) | Противоишемический растительный препарат мелилотин и способ его получения | |
RU2328302C2 (ru) | Средство, обладающее антигипоксическим действием |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
N231 | Notification of change of applicant | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20130319 Year of fee payment: 5 |
|
FPAY | Annual fee payment |
Payment date: 20140108 Year of fee payment: 6 |
|
FPAY | Annual fee payment |
Payment date: 20160203 Year of fee payment: 8 |
|
FPAY | Annual fee payment |
Payment date: 20170411 Year of fee payment: 9 |
|
FPAY | Annual fee payment |
Payment date: 20180405 Year of fee payment: 10 |
|
FPAY | Annual fee payment |
Payment date: 20190409 Year of fee payment: 11 |