KR20080088128A - Feed composite for a pig - Google Patents
Feed composite for a pig Download PDFInfo
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- KR20080088128A KR20080088128A KR1020070030579A KR20070030579A KR20080088128A KR 20080088128 A KR20080088128 A KR 20080088128A KR 1020070030579 A KR1020070030579 A KR 1020070030579A KR 20070030579 A KR20070030579 A KR 20070030579A KR 20080088128 A KR20080088128 A KR 20080088128A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
Abstract
Description
본 발명은 돼지를 사육하는데 사용되는 돼지 사료 조성물에 관한 것으로서, 특히, 분뇨에서 발생하는 환경 오염물질 및 악취유발물질을 감소시킬 수 있는 생균물질을 혼합하여 환경 환경오염물질 발생을 최소화 시킬 수 있는 친환경사료 조성물에 관한 것이다.The present invention relates to a pig feed composition used for breeding pigs, and in particular, by mixing a probiotic material that can reduce the environmental pollutants and odor-causing substances generated in the manure, environmentally friendly to minimize the generation of environmental environmental pollutants A feed composition.
현재 세계적으로 축산분뇨로 인한 환경오염이 대두되고 있으며, 우리나라도 양축업이 규모화 및 기업화가 됨에 따라 가축으로부터 배설된 분뇨가 수질 및 토양오염원이 되고 있다. 특히 돼지를 키우면서 발생하는 오염 물질을 감소시키는 것이 가장 시급한 문제로 대두되고 있는데, 그 중 환경오염의 원인물질로 많이 연구되고 있는 질소의 배설량을 줄이기 위한 영양적 처방이 그 예이다.At present, environmental pollution caused by livestock manure is emerging all over the world, and manure excreted from livestock is becoming a water quality and soil pollution source in Korea as the livestock industry is scaled up and commercialized. In particular, reducing the pollutants that occur while raising pigs has emerged as the most urgent problem, and nutritional prescription for reducing the amount of nitrogen excretion, which is being studied as a cause of environmental pollution, is an example.
종래 국내 암퇘지 분변으로부터 혐기적으로 분리된 유해 미생물 억제 활성을 갖는 신규 내산성 락토바실러스 속 미생물에 관한 특허출원이 공개(10-1998-0078353, 1998.11.16) 된 바 있는데, 이 발명은 자돈 등의 가축에 투여시 분변내 유산균 균총은 높게 유지하게 하지만 유해 대장균 균총은 감소시켜 어린 돼지의 설사를 방지하여 증체율을 높이는 락토바실러스 속 미생물 생균제에 관한 연구가 있었다. 즉, 종래 돼지용 사료 조성물은 돼지의 증체 또는 사료요구율 개선 등에 관한 연구가 대부분 이었다. Conventionally, a patent application for a novel acid-resistant Lactobacillus genus microorganism having anaerobic inhibitory activity isolated from domestic sows feces has been published (10-1998-0078353, 1998.11.16). Lactobacillus spp. Was maintained at a high level when administered to E. coli, but harmful E. coli strains were reduced to prevent diarrhea in young pigs. That is, the conventional pig feed composition was mostly a study on the improvement of feed rate or feed rate of pigs.
또한, 종래 생균제재는 돼지에게 유익한 미생물을 사료에 첨가하여 급여함으로써 장내 유익한 미생물을 우점하게 하고 유해한 미생물을 줄임으로써 돼지의 건강을 개선시키고 생산성을 증가시킴과 동시에 생균제가 자체적으로 분비하는 여러 가지 소화효소에 의해 돼지 소화기관내에서 사료의 소화율을 증가시키는 기능성 물질이다. In addition, the conventional probiotic material is added to the feed to feed the microorganisms beneficial to pigs to dominate the beneficial microorganisms in the intestine and reduce the harmful microorganisms to improve the health of pigs, increase productivity and at the same time the various digestive enzymes secreted by the probiotic itself It is a functional substance that increases the digestibility of feed in the pig digestive system.
이러한 생균제재의 기능성으로 인하여 돼지의 생산성 개선 및 환경 오염물질 배설 감소를 위한 연구 및 실험이 많이 이루어져 왔지만 사료의 생산과정 및 돼지의 소화기관내에서 생균제의 생존 가능성이 낮아 환경오염물질 배설 감소가 원활히 잘 이루어지지 않는 문제점을 가지고 있다.Due to the functionality of these probiotic materials, many researches and experiments have been conducted to improve the productivity of pigs and reduce the excretion of environmental pollutants. I have a problem that doesn't work.
그러나, 최근 친환경 축산농업에 대한 관심이 고조되어 있고, 추산분뇨에 관한 규제가 심화되고 있어 이에 대한 사료 조성물 연구 등이 중요하게 대두되고 있다. 특히 돼지 분변은 악취가 심한 관계로 이에 대한 연구가 특히 필요한 실정이다. 그래서, 본 연구자들은 사료생산 과정 및 돼지의 소화기관 내에서의 생존 가능성이 현저히 높은 생균제인 Bacillus sp . - EDS1 을 개발하게 되었다. 본 생균제 재, Bacillus sp . - EDS1 (KCTC18122P)은 발효식품인 메주로부터 분리한 포자형성 균으로서 내열성 및 내산성이 뛰어나고, 또한 여러 가지 가축에 유익한 소화효소를 분비하는 Bacillus sp . 계통의 균으로서 사료에 혼합되었을 때 제조과정에서의 생존력 및 돼지의 소화기관 내에서 생존력이 매우 뛰어나 친환경용 기능성 첨가 물질로 가능성이 매우 높다.However, recently, interest in environment-friendly livestock farming has increased, and regulations on estimated manure have been intensified, and thus, research on feed composition has emerged. In particular, pig feces have a bad smell, so research is needed. Therefore, we investigated Bacillus sp . , A probiotic with a high probability of survival in feed production and in the digestive tract of pigs . - EDS1 Was developed. This probiotic ash, Bacillus sp . - EDS1 (KCTC18122P) is as a spore forming bacteria isolated from the fermented food Meju excellent in heat resistance and acid resistance, and Bacillus to secrete digestive enzymes useful in a number of animal sp . When mixed with feed as a germ of the strain, the viability in the manufacturing process and the survivability in the digestive organs of pigs are very high, so it is very likely to be an environmentally friendly functional additive.
또한, 상기 Bacillus sp . - EDS1 (KCTC18122P)을 첨가한 사료에 단백질 수준을 낮추면서 합성아미노산을 첨가하여 소변이나 대변을 통하여 배설되는 환경오염물질인 질소를 감소시키는 본 발명을 연구하게 되었다. 특히 사료에 단백질 수준을 낮추면 불필요한 단백질의 함량이 감소되고, 그로 인하여 소변이나 대변에 배설되는 잉여의 질소 함량을 감소시킬 수 있게 된다. 그리고 조단백질 등의 영양소를 증가시키는 고단가의 사료를 적게 사용함으로써 그 경제성도 증가되는 효과가 있다. In addition, the Bacillus sp . - EDS1 (KCTC18122P) to reduce the protein level in the feed added synthetic amino acids to reduce the nitrogen, an environmental pollutant excreted through urine or feces was studied. In particular, lowering protein levels in feed reduces unnecessary protein content, thereby reducing excess nitrogen content excreted in urine or feces. In addition, by using less expensive foods that increase nutrients such as crude protein, the economics are also increased.
위에서 설명한 상기 두 가지 사료 조성물의 영양적 방법은 서로 다른 기작으로 질소 배설을 감소시킬 수 있다. 즉, 첫 번째 방법은 사료 조성물 중의 조단백질 함유량을 감소시켜 소변이나 대변으로 배설되는 질소를 감소시킬 수 있고, 그리고 두 번째 방법은 Bacillus sp . - EDS1 (KCTC18122P) 생균제의 첨가를 통하여 돼지 장내에서 소화되고 남은 대변이나 소변으로 배설되는 질소를 감소시킴으로써, 위 두 가지 영양적 방법을 동시에 적용하면 상호상승 작용에 의해서 질소 배설의 감소 효과를 극대화시킬 수 있다.The nutritional method of the two feed compositions described above can reduce nitrogen excretion by different mechanisms. That is, the first method can reduce the crude protein content in the feed composition to reduce the nitrogen excreted in the urine or feces, and the second method is Bacillus sp . - EDS1 (KCTC18122P) By adding probiotics to reduce the nitrogen that is digested in the intestine and excreted in the remaining feces or urine, simultaneous application of these two nutritional methods can maximize the effect of reducing nitrogen excretion by synergistic action. .
따라서, 본 발명은 상술한 제반 문제점을 해결하고자 안출된 것으로, 본 발명의 구성은 질소 배출을 감소시키기 위해 Bacillus sp. - EDS1 (KCTC18122P)을 주(主) 균으로 하는 생균제재를 사료 조성물에 첨가하고, 동시에 사료의 단백질 함량을 낮추기 위해 합성아미노산제재를 첨가한 사료 조성물을 돼지에 급여하여 환경오염의 원인물질인 질소의 배설을 최대한 감소시킬 수 있는 친환경 가축 사료 조성물의 제공을 그 목적으로 한다.Accordingly, the present invention has been made to solve the above-mentioned problems, the composition of the present invention to reduce the nitrogen emissions Bacillus sp. -Nitrogen , the causative agent of environmental pollution, was added to the feed composition by adding probiotics containing EDS1 (KCTC18122P) to the feed composition, and at the same time feeding pigs with the feed composition containing synthetic amino acids to lower the protein content of the feed. It is an object of the present invention to provide an environment-friendly livestock feed composition that can reduce the excretion of maximally.
상기와 같은 목적을 달성하기 위한 본 발명에 따른 친환경 사료 조성물의 구성은, 돼지용 사료 조성물에 Bacillus sp . - EDS1 (KCTC18122P)을 더 함유하게 하여 질소배설을 감소시키는 친환경 돼지용 사료 조성물에 관한 것이다.The composition of the eco-friendly feed composition according to the present invention for achieving the above object, Bacillus in a feed composition for pigs sp . - EDS1 It relates to an environment-friendly pig feed composition to further contain (KCTC18122P) to reduce nitrogen excretion.
본 발명에 따른 다른 구성은 사료의 조단백질 함유량을 낮추면서 상기 Bacillus sp . - EDS1 (KCTC18122P) 0.01∼0.1% 중량%와 메치오닌 0.01∼1 중량%, 라이신 0.01∼1.5 중량%, 트레오닌 0.01∼1 중량%, 트립토판 0.01∼1 중량%의 아미노산을 첨가하여 질소배설을 감소시키는 친환경 돼지용 사료 조성물에 관한 것이다.According to another aspect of the present invention, the Bacillus sp . - EDS1 (KCTC18122P) Eco-friendly pig for reducing nitrogen excretion by adding 0.01 to 0.1% by weight, 0.01 to 1% by weight of methionine, 0.01 to 1.5% by weight of lysine, 0.01 to 1% by weight of threonine, and 0.01 to 1% by weight of tryptophan A feed composition.
본 발명에 따른 Bacillus sp . - EDS1을 함유한 친환경 가축 사료 조성물은 옥수수 50∼80 중량%, 대두박 10∼25 중량%, 우지 0.5∼3 중량%, 석회석 0.5∼2 중량%, 인산칼슘 0.5∼2 중량%, 소금 0.2∼0.5% 중량%, 메치오닌 0.01∼1 중량%, 라이신 0.01∼1.5 중량%, 트레오닌 0.01∼1 중량%, 트립토판 0.01∼1 중량%, 미네랄 첨가제 0.05∼0.15 중량%, 비타민 첨가제 0.05∼0.15 중량%, 콜린 0.05∼0.2 중량% 및 Bacillus sp . - EDS1 0.01∼0.1% 중량%로 이루어져 질소배설을 감소시키는 것을 특징으로 하는 친환경 돼지용 사료 조성물에 관한 것이다. Bacillus according to the present invention sp . - Green fodder compositions containing corn EDS1 50-80% by weight, 10 to 25% by weight soybean meal, tallow from 0.5 to 3% by weight, limestone 0.5 to 2% by weight, 0.5 to 2 percent by weight of calcium phosphate, salt 0.2 to 0.5 % Wt%, methionine 0.01-1 wt%, lysine 0.01-1.5 wt%, threonine 0.01-1 wt%, tryptophan 0.01-1 wt%, mineral additive 0.05-0.15 wt%, vitamin additive 0.05-0.15 wt%, choline 0.05 -0.2 wt% and Bacillus sp . - EDS1 will consist of 0.01~0.1 wt% of the feed composition for environmentally friendly pig, characterized in that to reduce nitrogen excretion.
상기 사료 조성물 중 조단백질 함유하는 영양소는 옥수수 50∼80 중량%, 대두박 10∼25 중량%인데 종래 일반적인 사료에는 대두박이나 옥수수의 함유량이 지나치게 높아 조단백질의 함유량이 과잉 공급되는 경우가 많았다. 따라서 본 발명에서의 친환경 사료 조성물은 미국 사양표준지침 (NRC, 1998)을 기초로 하여 3,400 ME kcal/kg, 14 중량%의 조단백질을 함유하였으나 아래 도표 1의 종래 통상의 대조구 사료조성물은 3,400 ME kcal/kg, 18 중량%의 조단백질을 함유하여 그 조단백질의 함유량을 4중량%가 더 많이 함유되어 있다.Nutrient-containing nutrients in the feed composition are from 50 to 80% by weight of corn and 10 to 25% by weight of soybean meal. However, in the conventional general feed, soybean meal or corn content is too high, and the crude protein content is often excessively supplied. Therefore, the eco-friendly feed composition according to the present invention contained 3,400 ME kcal / kg, 14 wt% of crude protein based on the American Standard Guideline (NRC, 1998), but the conventional conventional control composition of Table 1 is 3,400 ME kcal. / kg, containing 18% by weight of crude protein, 4% by weight of the crude protein content is higher.
이하, 본 발명에 따른 생균제를 함유한 친환경 가축사료 조성물의 구성과 작용 효과를 바람직한 실시예를 통해 구체적으로 설명한다.Hereinafter, the configuration and effect of the eco-friendly livestock feed composition containing a probiotic according to the present invention will be described in detail through the preferred embodiment.
[실시예]EXAMPLE
본 발명에 실시한 예에 따른 생균제를 함유한 친환경 가축사료 조성물은 육성돈 배합사료에 쓰이는 것으로 본 실시예는 상기 친환경 가축사료의 효능을 검증하기 위하여 실시하였다.Eco-friendly livestock feed composition containing a probiotic according to the embodiment of the present invention to be used in the growing feed compound feed This embodiment was carried out to verify the efficacy of the eco-friendly livestock feed.
본 실시예에 사용된 가축은 체중 33.71kg의 3원 교잡종 (Landrace×Yorkshire×Duroc) 육성돈으로서, 24두를 공시하였다. 사양시험은 단국대학교 부설 실험동물사육실에서 실시되었으며, 시험설계는 표 1과 같이 옥수수-대두박 위주의 사료에 미국사양표준지침 (NRC, 1998)의 영양소 요구량에 따라 처리한 군 (이하 대조구), 대조구 사료에 단백질 수준을 낮추고 생균제, Bacillus sp . - EDS1 0.05중량% 첨가한 첨가군 (이하 처리구)의 2개 군으로 처리를 하여 각 처리 당 여섯 번의 반복시험을 거쳤다. 상기 생균제인 Bacillus sp . - EDS1 (KCTC18122P)은 발효식품인 메주로부터 분리한 포자형성 균이다. 본 시험에 사용된 Bacillus sp . - EDS1 (KCTC18122P)은 2007년 3월 5일에 한국생명공학연구원(KCTC)에 기탁을 하였고, 그 한국생명공학연구원(KCTC)로부터 KCTC - 18122P라는 미생물 기탁번호를 부여 받았다. 상기 생균제인 Bacillus sp. - EDS1 (KCTC18122P)은 내열성 및 내산성이 뛰어나고, 또한 여러 가지 가축에 유익한 소화효소를 분비하는 Bacillus sp. 계통의 균으로서, 돼지용 사료에 혼합하였을 때 그 제조과정에서의 생존력 및 돼지의 소화기관 내에서 생존력이 매우 뛰어난 장점이 있다.The livestock used in this example was a three-way hybrid (Landrace × Yorkshire × Duroc) growing pig with a weight of 33.71 kg, and 24 heads were disclosed. The specification test was conducted in the experimental animal breeding room at Dankook University, and the test design was treated in the corn-soybean meal-oriented feed according to the nutrient requirements of the American National Standard Guideline (NRC, 1998) and control. Lower protein levels in feed and probiotic, Bacillus sp . - EDS1 0.05 by the process into two groups in% by weight was added was added group (the treatment group) underwent six repetitions for each tested treatment. Bacillus , the probiotic sp . - EDS1 (KCTC18122P) is a spore forming bacterium isolated from fermented food meju. Bacillus used in this test sp . - EDS1 (KCTC18122P) was deposited on March 5, 2007 with the Korea Research Institute of Bioscience and Biotechnology (KCTC) and received a microbial accession number of KCTC-18122P from the Korea Research Institute of Bioscience and Biotechnology (KCTC). The probiotic Bacillus sp. - EDS1 (KCTC18122P) has excellent heat and acid resistance, and also produces Bacillus sp. As a strain of the strain, when mixed with the feed for pigs has the advantage of viability in the manufacturing process and viability in the digestive system of the pig is very excellent.
상기 Bacillus sp . - EDS1 0.05중량% 첨가한 사료 조성물의 구체적인 사료 조성물 재료 및 그 함유량을 기재하면 아래 도표와 같다. Bacillus sp . - when a specific substrate feed composition materials and their content of 0.05% by weight was added EDS1 feed compositions shown in Table below.
상기 친환경사료는 미국 사양표준지침 (NRC, 1998)을 기초로 하여 3,400 ME kcal/kg, 14 중량%의 조단백질을 함유하였고, 대조구 사료는 3,400 ME kcal/kg, 18 중량%의 조단백질을 함유하였다. 시험사료는 가루 사료의 형태로 제공하였고, 체중을 기준으로 하여 유지에너지의 2배를 급여 하였으며, 물은 자동급수기를 이용하여 자유로이 먹을 수 있도록 하였다. The environmentally friendly feed contained 3,400 ME kcal / kg, 14 wt% crude protein, and the control diet contained 3,400 ME kcal / kg, 18 wt% crude protein, based on US standard guidelines (NRC, 1998). The test feed was provided in the form of powdered feed, twice the maintenance energy based on the weight, and the water was freely fed using the automatic water dispenser.
[시험예][Test Example]
영양소 소화율을 측정하기 위하여 표시물로서 산화크롬(Cr2O3)을 사료내 0.2% 첨가하였다. 분은 전분 채취법으로 수집하여 분석에 이용하였고, 분과 뇨의 배설량은 매일 채취하여 무게를 측정하였다. 모든 화학분석은 AOAC (1995)에 의해 분석하였다.To measure the nutrient digestibility, chromium oxide (Cr 2 O 3 ) was added as 0.2% in the feed. Starch was collected by starch sampling and used for analysis. The excretion of minute and urine was collected daily and weighed. All chemical analyzes were analyzed by AOAC (1995).
분뇨 내 발생하는 악취물질 측정은 신선한 분 100g 과 뇨 100g을 혼합하여 7일간 발효 후 측정하였다. 모든 자료는 SAS (1996)의 General Linear Model Procedure를 이용하여 분산분석을 실시하였다.Odor substances measured in the manure were measured after fermentation for 7 days by mixing 100g of fresh manure and 100g of urine. All data were analyzed by ANOVA using the General Linear Model Procedure of SAS (1996).
그 결과 하기 표2에서와 같이, 친환경 사료의 건물 소화율이 대조구에 비하여 4.7% 개선되었으며, 분배설량이 11% 감소하였고 분내 질소 배설량이 11.4% 감소하였다. 뇨 배설량도 친환경 사료에서 7.7% 감소하였으며, 뇨내 질소 배설량은 26.1% 감소하였다. 총 분뇨 배설량 및 총 질소 배설량은 친환경 사료에서 8.1% 및 24.2% 감소하였다. 또한 분뇨 내 암모니아 가스 발생량도 친환경 사료에서 26.7% 감소하였다.As a result, as shown in Table 2, the dry digestibility of the eco-friendly feed was improved by 4.7% compared to the control, the distribution snowfall was reduced by 11%, and the minute nitrogen excretion was reduced by 11.4%. Urine excretion also decreased by 7.7% in environmentally friendly feeds, and urinary nitrogen excretion decreased by 26.1%. Total manure excretion and total nitrogen excretion were reduced by 8.1% and 24.2% in green feed. In addition, the amount of ammonia gas in the manure was also reduced by 26.7% in eco-friendly feed.
본 발명은 상술한 특정의 바람직한 실시예에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형의 실시가 가능한 것은 물론이고, 그와 같은 변경은 청구범위 기재의 범위 내에 있게 된다.The present invention is not limited to the above-described specific preferred embodiments, and various modifications can be made by any person having ordinary skill in the art without departing from the gist of the present invention claimed in the claims. Of course, such changes will fall within the scope of the claims.
이상 설명한 바와 같이 본 발명에 의하면,As described above, according to the present invention,
돼지용 사료 조성물에서 조단백질 함유량을 낮추면서 상기 Bacillus sp . - EDS1 (KCTC18122P) 0.01∼0.1% 중량%와 메치오닌 0.01∼1 중량%, 라이신 0.01∼1.5 중량%, 트레오닌 0.01∼1 중량%, 트립토판 0.01∼1 중량%의 아미노산을 첨가한 본 발명은, 돼지의 소화기관 내에서 생존 가능성이 현저히 높은 생균제, Bacillus sp . - EDS1 을 사료 조성물에 혼합하고, 아울러 상기 사료 조성물에 조단백질 수준을 낮추면서 합성아미노산을 첨가함으로써, 일반적으로 소변 또는 대변을 통하여 배설되는 환경오염물질인 질소 함량의 감소에 탁월한 효과가 있게 하고, 또한 이러한 질소 함량의 감소에 따른 돈사 내의 악취 감소 및 섭취된 사료효율의 극대화로 친환경 축산농업 및 농가소득에 기여되는 효과가 있게 하였다. Bacillus while lowering crude protein content in feed composition for pigs sp . -0.01 % to 0.1% by weight of EDS1 (KCTC18122P), 0.01 to 1% by weight of methionine, 0.01 to 1.5% by weight of lysine, 0.01 to 1% by weight of threonine, and 0.01 to 1% by weight of tryptophan. Bacillus , a probiotic with a high survival potential in the digestive tract sp . - EDS1 By adding synthetic amino acids to the feed composition and lowering crude protein levels to the feed composition, thereby providing an excellent effect on the reduction of nitrogen content, an environmental pollutant that is normally excreted through urine or feces. Reduction of odor in pigs and maximization of feed efficiency by reducing the amount contributed to eco-friendly livestock farming and farm household income.
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