KR20080039371A - An antibacterial and antifungal composition containing dracocephalum foetidum extracts, sophora alopecuroides extracts or fractions thereof - Google Patents

Abstract

An extract of Dracocephalum foetidum is provided to show antibacterial activity against bacteria and fungi on the skin surface, bacteria causing food decay and food poisoning, Streptococcus mutans and anti-biotics resistant bacteria, thereby being usefully used as an antibacterial composition for sterilizing the skin surface, preventing the food decay, food poisoning and periodental diseases and as a natural antibacterial agent for a filter of an air cleaning article without side effect. An antibacterial composition comprises a refined oil ingredient of Dracocephalum foetidum, which is obtained by extracting dried Dracocephalum foetidum through distillation to obtain an extract, cooling down the extract and then isolating a refined oil fraction of an upper layer therefrom. The composition is a skin external application composition, a cosmetic composition, or an oral composition. An air cleaning article comprises the extract of Dracocephalum foetidum as an effective ingredient. Further, the composition as a skin external application composition is in a form of solution, spray, patch, pad, cream, ointment and gel.

Description

Antibacterial and antifungal composition containing Dracocephalum foetidum extracts, Sophora alopecuroides extracts or fractions

The present invention relates to an antimicrobial composition comprising Dracosepalum Poetidum extract, Goya extract or fractions thereof.

Antibiotics are substances that have a selective toxicity that kills microorganisms and are not toxic to humans or animals and are not inactivated by enzymes in the body. These are mainly due to DNA replication, transcription and translation of genetic information, and electronic energy. It inhibits transport, biosynthesis of cell walls, and the like, through the mechanism of inhibiting the growth of microorganisms.

The antibiotics were first discovered by Pen Alexillor Fleming in 1929 and by Flory et al. In 1939 for the first time to isolate and purify penicillin from a culture of Penicillium chrysogenum . It has been known as a miracle medicine by saving the lives of numerous patients with it. Since 1959, research has been conducted in earnest, and benzylpenicillin, streptomycin, tetracycline, and macrolide erythromycin have been developed. . Since penicillin was first developed by synthetic methods in 1965, semisynthetic penicillin, methicillin, and the like, and in 1973, cephazoline, cephazoline.

However, shortly after penicillin was used, bacteria resistant to it began to emerge. The first penicillin-resistant strains were reported in Australia in 1967, and soon bacteria that became resistant to methicillin or cefazoline began to appear, causing serious problems. In addition, the first organisms resistant to various antibiotics appeared in South Africa in 1977.In the 1980s, cephalosporin preparations and numerous antibiotics such as quinolone, carbapenem, monobactam, and glycosides were developed and Was used. However, after this period, penicillin-resistant pneumococci and methicillin-resistant staphylococcus aureus (MRSA) began to become a problem all over the world, and vancomycin-resistant aureus (VRE) emerged in the 1990s, leading to the problem of antibiotic-resistant bacteria. It has emerged as a medical concern.

In addition, since most antibiotics have multiple chiral centers and complex structures such as macrolides or peptides, they are difficult to synthesize, and these synthetic methods also have very low economical efficiency compared to fermentation methods. . Therefore, it is useful to obtain new antibiotics by finding new substances or their intermediates from natural products or by changing their substituents.

In addition, existing antibiotics specifically act on the microorganisms, such as peptidoglycan, bacterial ribosome, and bacterial DNA gyrase. The bacteriocidal kills (bacteriostatic) and has an antimicrobial effect. In this case, there has been a problem that it can cause fatal damage even to beneficial bacteria. Therefore, by maximally protecting the normal microflora that is always present on the skin or mucous membranes of the body, it inhibits infection caused by the invasion of external bacteria and at the same time removes only Gram positive or Gram negative harmful bacteria. The development of selective antimicrobial ingredients that can be done in a wide range of conditions for synthetic or natural products.

On the other hand, modern people have a growing tendency to prefer natural remedies from nature due to the rejection of chemical synthetic products around the world.

The present inventors have a therapeutic and preventive effect against the harmful bacteria and fungi that we encounter in everyday life, for example, the cause of oral disease, dermatophytes and resistant bacteria methicillin resistant Staphylococcus aureus (MRSA) infection As a result of continuing research to develop an antimicrobial agent, some of the extracts of Mongolian-derived Dracosepalum poetitum, gourd fruit and fractions thereof have excellent antimicrobial activity and have no side effects. The present invention was completed by confirming.

It is an object of the present invention to provide an extract, preferably an essential oil component of Dracosepalum poetitum.

Still another object of the present invention is to provide an antimicrobial composition comprising the extract of Dracosepalum poetidim, gourd extract, fractions thereof, or mixtures thereof as an active ingredient.

In order to achieve the above object, the present invention provides an extract, preferably essential oil component of Dracosepalum Poetidum.

The present invention also provides an antimicrobial composition comprising the extract of Dracosepalum poetidum, gourd extract, fractions thereof, or mixtures thereof as an active ingredient.

The extract of Dracosepalum Poetidum of the present invention, gourd extract and fractions thereof have antimicrobial activity against antibiotic resistant bacteria as well as bacteria and fungi causing skin surface bacteria and fungi, food decay and food poisoning, Dracosepalum The extract of Poetidum exhibits antimicrobial activity due to its volatile components, so it cleans, sterilizes and sterilizes the skin surface, prevents food spoilage, prevents food poisoning, and prevents oral diseases and tooth decay, as well as air. As a natural antibacterial agent for filters of cleaning products, it can be usefully used without side effects.

Hereinafter, the present invention will be described in detail.

The present invention provides an extract, preferably essential oil, of Dracosepalum poetitum.

The Dracocephalum Poetidum foetidum is a herbaceous plant belonging to the Lamiaceae family. Mongolian nomads are known to have used the leaves and flowers of this plant to treat arthritis, species, edema, diarrhea and vomiting.

The extract of Dracosepalum poetitum of the present invention, preferably the essential oil component, may be extracted using a general extraction method known in the art or by the following extraction method:

(a) extracting the dried Dracosepalum poetidum by distillation to obtain an extract; And

(b) separating the essential oil component of the upper layer after cooling the extract.

In step (a), the dried Dracosepalum poetidim is extracted by distillation.

The distillation method is a method of heating the liquid to the boiling point, cooling the evaporated material to form a liquid again, in the present invention preferably steam distillation can be used. Steam distillation is a distillation method in which water vapor is blown into a substance having a high boiling point and hardly dissolved in water to cool the vapor of the substance flowing out with water vapor at a temperature lower than the boiling point of the substance, and then condensed as a mixture with water and separated from it. Alternatively, water may be added and heated instead of blowing water vapor. Therefore, the conventional distillation method is used for separation and purification of organic substances that may decompose or organic compounds having a vapor pressure considerably higher than the boiling point. In particular, it is conveniently used for separating into two liquid layers after condensation such as oil.

In the present invention, the dried Dracosepalum poetidum is placed in a still, preferably water is supplied to supply steam. The amount of water to be used is preferably 5 to 10 weight times of Dracosepalum poetidim to be extracted, and the distillation time is preferably 2 to 24 hours, more preferably 3 hours.

Next, in step (b), after cooling the extract obtained in step (a) to separate the essential oil components of the upper layer.

The extract is cooled to room temperature to be separated into an aqueous component and an oil component, and the essential oil component formed on the upper layer is separated using a separatory funnel, etc., thereby preparing the dracocepalum poetitum essential oil component of the present invention.

In another aspect, the present invention provides a composition for antimicrobial comprising the extract of the extract, fractions or mixtures thereof as an active ingredient.

Sophora alopecuroides ) is An outpost of legumes, a legume plant. Doduja does not grow in Korea but in Mongolia and China. In Mongolian folk remedies, this plant was used as an antidote, as an antipyretic, and also as a skin rash. Ingredients of gourds include matrine, oxymatrine, oxysophocarpine, cytisine, sopocarpine, sophoridine, nicotine, Sophoramine and alloperine are known and described by Zhongguo Zhong Yao Za Zhi 1997, 22 (12): 740-3, J Nat Prod 2000, 63 (2): 190-2, J Chromatogr A. 1999, 857 (1-2): 303-11], anti-inflammatory and anti-allergic effects [Zhongguo Yao Li Xue Bao 1989, 10 (4): 360-5], improvement of arrhythmia [Yao Xue Xue Bao 1989, 24 (2): 147-50].

The gourd extract and fractions thereof may be prepared from the gourd using extracts, separation and purification methods known in the art or according to the present invention.

The gourd extract is preferably an extract solvent of water, alcohol, hexane, methylene chloride, ethyl acetate, butanol or a mixed solvent thereof, more preferably water, C ₁ to C ₄ alcohol or a mixed solvent thereof. Preferably it can be prepared using methanol. At this time, the preferred amount of the extraction solvent is 5 to 20 times by weight of the gourd to be extracted, the extraction time is preferably 2 to 24 hours, more preferably 3 hours.

In addition, the fraction of the gourd extract is preferably a fraction of the methanol extract of gourd, which is dissolved in water methanol extract of the gourd, hexane, methylene chloride, ethyl acetate, by a conventional method using a separatory funnel, etc. It may be prepared by fractionation with butanol in order.

The extracts and fractions may be further subjected to further steps of filtration and purification.

The Dracosepalum poetidum extract of the present invention, gourd extract and fractions thereof are known as Candida albicans ( C. albicans ), Saccharomycetes known as bacteria or fungi on the surface of the skin, causing bacteria and food poisoning or caries. My process in serenity busy (S. cerevisiae), Escherichia coli in sherry (E. coli), Bacillus subtilis (B. subtilis), Streptococcus mutans (S. mutans), Staphylococcus fringing-house (S. aureus Disc diffusion and minimal inhibition of E. hirae , M. luteus and methicillin resistant staphylococcus aureus (MRSA). This can be confirmed by measuring the concentration. In particular, Dracosepalum Poetidum extract has a high antimicrobial effect against S. mutans known as cavities, and the antimicrobial effect of Dracosepalum Poetidum extract is also shown by its volatile components. Can be.

Therefore, Dracosepalum poetidum extract of the present invention, gourd extract and fractions thereof are excellent antibacterial effect, and can be useful for the purpose of removing bacteria or fungi because there is no side effect.

The Dracosepalum poetithium extract, gourd extract, fractions thereof or mixtures thereof are used for the purpose of efficiently removing harmful bacteria and fungi, and are not particularly limited in the formulation. It may contain at least one active ingredient having the same or similar function in addition to the Tidum extract, gourd extract, fractions thereof, or mixtures thereof.

The composition may be an external composition for skin, and specifically, may be prepared as a solution, suspension, spray, patch, pad, cream, ointment, gel.

In addition to the active compounds, solutions may be prepared by conventional excipients such as solvents, solubilizers and emulsifiers such as water, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol, dimethylformamide, oils, especially cottonseed oil, peanut oil, camellia oil, aloe vera, glycerin, natural corn seed oil, olive oil, castor oil, almond oil and sesame oil, glycerol, glycerol form alcohol, tethyrahydro Fatty acid esters of furfuryl alcohol, polyethylene glycol and sorbitan, or mixtures of these materials, and may include methyl or propyl-p-hydroxybenzoate or sorbic acid as a preservative.

Suspending agents, in addition to the active compounds, include conventional excipients, for example liquid diluents such as water, ethyl alcohol and propylene glycol, polyethylene glycol, and suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol, sorbitan Esters, cellulose derivatives and hydrogenated edible oils), microcrystalline cellulose, aluminum metahydroxy, bentonite, agar and injectable esters such as tragacanth, ethyloleate or mixtures of these materials.

Ointments, creams and gels may be used in addition to the active compounds, as usual excipients, for example animal and vegetable fats, wax paraffins, starches, targacanths, cellulose derivatives, polyethylene glycols, silicones, bentonite, silicic acid, talc and zinc oxide or these substances It may contain a mixture of.

In addition, the composition may be a cosmetic composition, it is not particularly limited in the formulation may have a conventional formulation. Skin Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Essence, Nutrition Essence, Pack, Liquid or Solid Soap, Shampoo, Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser and bathing agent.

The cosmetic composition, in addition to Dracosepalum Poetidum extract, gourd extract, fractions or mixtures thereof, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, blowing agents ( foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metalion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents Or adjuvants commonly used in the field of dermatology, such as lipid vesicles. The ingredients may also be introduced in amounts generally used in the field of dermatology.

In addition, the composition may be a composition for oral cavity for the purpose of preventing oral diseases or oral cleansing by removing bacteria and fungi in the oral cavity, and may have a conventional formulation without being particularly limited in the formulation. Specifically, it may have a formulation such as toothpaste, mouthwash or mouthwash.

The composition for oral cavity may contain various bases and additives necessary for formulating according to the formulation, in addition to Dracosepalum Poetidum extract, gourd extract, fractions thereof, or mixtures thereof, the type and amount of these components Can be easily selected. For example, when the formulation of the composition for oral cavity is a toothpaste, it can be prepared by adding an abrasive, a humectant, a foaming agent, a binder, a sweetening agent, a pH adjusting agent, a preservative, an active ingredient, a fragrance, a brightener, a pigment, a solvent and the like.

In vitro ( in vitro) and in vivo (in Based on the experiments in vivo ), Dracosepalum Poetidum extract may be included in the total weight of the antimicrobial composition of the present invention preferably 0.00001 to 50 parts by weight, more preferably 0.0001 to 10 parts by weight, most preferably 0.001 to 0.1 parts by weight. have. In addition, the extract of gourd or its fraction may be included in the total weight of the antimicrobial composition of the present invention preferably 0.0001 to 50 parts by weight, more preferably 0.001 to 10 parts by weight, most preferably 0.01 to 5 parts by weight.

The present invention also provides an air-cleaning article comprising Dracosepalum Poetidum extract, Dulpenia extract, fractions thereof or mixtures thereof. The air cleaning article includes a filter for an air cleaner or a central air conditioning apparatus, and the extract or fraction is applied to the air cleaning article using any method known in the art, such as coating the existing filter in the form of a composition Can be used.

Hereinafter, the present invention will be described in detail by examples and production examples.

However, the following Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples.

< Example  1> Draco Cefalum Poetidum  Essential Oils Manufacturing

500 g of dried Dracosepalum poetitum flower and 500 g of starch were placed in a steam still, heated to boil water, distilled for 3 hours, cooled to room temperature, and 1 ml of an essential oil component of the separated upper layer was obtained.

< Example  2> Gourd  Preparation of Extract

1 kg of dried olivine outpost was put in an extraction bag, and 10 vol of 100% methanol was immersed and refluxed for 3 hours, cooled to room temperature, and filtered through a filter paper. The extract was concentrated using a concentrator under reduced pressure until the solution was completely evaporated, whereby 9.5 g of the extract was obtained as crystals.

< Example  3 to 7> Gourd  Of extract Hexane Fraction  Produce

5 g of the extract prepared in <Example 2> was well suspended in 1 L of distilled water, and then mixed with hexane, methylene chloride, ethyl acetate and butanol in turn, and left to stand along with the supernatant to prepare respective fractions. The fractions were concentrated using a concentrator under reduced pressure until the solution was completely evaporated, whereby hexane fraction (Example 3) 800 mg of methanol extract (Example 3), 750 mg of methylene chloride fraction (Example 4), ethyl acetate fraction ( Example 5) 850 mg and 1 g of butanol fraction (Example 6) and 1.65 g of water fraction (Example 7) were obtained.

< Experimental Example  1> Antibacterial test by disk diffusion method

In order to confirm the antimicrobial activity of the essential oil component of Dracosepalum poetidum and gourd extract and fractions of Examples 1 to 7 prepared by the above method, a disk diffusion method was performed as follows.

Candida albicans (C. albicans) I am grown overnight in liquid culture medium, in my process serenity busy as Saccharomyces (S. cerevisiae), Escherichia coli in sherry (E. coli), Bacillus subtilis (B. subtilis), Streptococcus mu S. mutans , S. aureus , E. hirae , M. luteus and methicillin resistant methicillin resistant (MRSA) staphylococcus aureus ) were diluted to 1 / 1,000, respectively, and 100 μl of them were spread on agar medium. 5 μl of a solution dissolved in ethanol so that the concentration of the extract or fractions prepared in Examples 1 to 7 was soaked in ethanol, about 8 mm in diameter, and then placed on agar medium to spread on agar medium. After culturing at 37 ° C. or 30 ° C. for 24 hours or 48 hours, the antimicrobial activity was measured by measuring a part (clear zone) in which bacterial growth was inhibited.

 The results are shown in Table 1.

Antimicrobial Activity against the Different Bacteria by the Size of the Clear Zone Strain Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 C. albicans +++ + - + ++++ - - S. cerevisiae +++ ++ - + ++++ - - E. coli + + - - - - - B. subtilis +++++ ++ - ++ - - - S. mutans +++++ ++ - - - - - S. aureus +++++ ++ - +++ - - - E. hirae +++++ +++ + ++++ - - + M. luteus +++++ ++ - ++ - - - MRSA +++ ++ - +++ - - -

(No inhibitory effect:-, 7 to 20 mm: +, 21 to 40 mm: ++, 41 to 60 mm: +++, 61 to 80 mm: ++++, bacterial absence: +++++)

As shown in Table 1, in the case of Dracosepalum Poetidum essential oil, except for E. coli showed a large clear zone or almost no growth of bacteria, it was confirmed that the antimicrobial activity is very good. It showed high antimicrobial activity.

In addition, the extract of the oleander extract showed the highest antimicrobial activity against E. hirae , and Example 4 (methylene chloride fraction) among the fractions showed the high activity against E. hirae , S. aureus , MRSA, Example 5 (Ethyl Acetate Fraction) is specifically yeast C. albicans And very high activity against S. cerevisiae .

< Experimental Example  2> by volatilization Draco Cefalum Poetidum  Antibacterial Test of Essential Oils

In order to confirm the antimicrobial activity of the dracocepalum poetidium essential oil component prepared in Example 1 by the volatile substance, a modified disk diffusion method was performed as follows.

C. albicans , S. cerevisiae , E. coli , B. subtilis, S. mutans , S. aureus , E. hirae , M. luteus and MRSA grown overnight in liquid medium were diluted 1 / 1,000, 100 of which Μl was spread on agar medium and spread. Wet round filter paper with a diameter of 8 mm with 5 µl of ethanol dissolved in ethanol so that the concentration of Dracosepalum Poetidum essential oil was 20 mg / ml, and then attached to the inside of the upper lid of each petri dish in which the strains were cultured. The agar was volatilized. After culturing at 37 ° C. or 30 ° C. for 24 hours or 48 hours, the antimicrobial activity was measured by measuring a part (clear zone) in which bacterial growth was inhibited.

 The results are shown in Table 2.

Antimicrobial Activities of Volatile Dracosepalum Poetidum Essential Oils on Various Bacteria in Clear Zones Strain Example 1 C. albicans +++ S. cerevisiae ++++ E. coli - B. subtilis +++++ S. mutans +++++ S. aureus +++++ E. hirae +++++ M. luteus +++++ MRSA +++

(No inhibitory effect:-, 7 to 20 mm: +, 21 to 40 mm: ++, 41 to 60 mm: +++, 61 to 80 mm: ++++, bacterial absence: +++++)

As shown in Table 2, the volatile components in the essential oil component of Dracosepalum Poetidum prepared in Example 1 showed a wide clear zone or almost no growth of all bacteria used in the experiment except E. coli . It could not be confirmed that there is a strong antimicrobial activity against the bacteria.

The minimum inhibitory concentration of <Experiment 3> The antimicrobial activity seen sample (MIC, Minimum Inhibitory Concentration Measurement

The minimum concentration (MIC) of the microorganisms which did not grow as follows was obtained for the examples in which the antimicrobial activity was confirmed by the disk diffusion methods of Experimental Examples 1 and 2.

The concentrations of the extracts or fractions prepared in Examples 1, 2, 4 or 5 were lowered to 12 concentrations starting at 500 μg / ml and continuously diluted to 2 fold concentrations. Incubation for 18 hours adjusted the A ₂₆₀ to 0.2. The extracts or fractions and strains were mixed in equal amounts in 96-well microplates and incubated for 24 hours. MIC was added to 50 μl of 0.1 mg / ml p-iodonitrotetrazolium violet (p-INT) in each well, incubated for 2 hours, and then reddish by the formation of INT formazan. This was determined as the minimum undeveloped concentration.

The results are shown in Table 3.

MIC measurement result (unit: µg / ml) Strain Example 1 Example 2 Example 4 Example 5 C. albicans 7.8 500 500 7.8125 S. cerevisiae 7.8 500 500 7.8125 E. coli 3.4 250 250 - B. subtilis 1.7 31.25 31.25 - S. mutans 3.4 31.25 - - S. aureus 3.4 31.25 15.625 - E. hirae 0.425 15.625 7.8125 - M. luteus 15.625 31.25 31.25 - MRSA 3.4 31.25 - -

(No Inhibition:-)

As shown in Table 3, both the dracocepalum poetidium essential oil component prepared in Example 1 and the gourd extract prepared in Example 2 had low MIC values for all harmful bacteria used in the above experiments. It was confirmed that the activity is excellent, Drakosepalum Poetidum essential oil component was confirmed that the antimicrobial activity is better than the extract. In addition, among the fractions, Example 4 (methylene chloride fraction) and Example 5 (ethyl acetate fraction) showed the highest antimicrobial activity, in particular, Example 5 showed a very good antimicrobial effect against fungi.

Therefore, it was confirmed that Dracocepalum poetidum essential oil component, gourd extract and fractions thereof can be usefully used as an antimicrobial composition.

The preparation example for the antimicrobial composition of this invention is illustrated below.

< Formulation example  1> Cosmetics  Preparation of the composition

<1-1> Preparation of Cream

0.1 parts by weight of the essential oil component of Dracosepalum poetidum, gourd extract, fractions thereof, or mixtures thereof of the present invention

Cetostearyl alcohol 2.8 parts by weight

Milnan 2.6 parts by weight

Stearic acid 1.4 parts by weight

2 parts by weight of glycerol phosphate monostearate

Fiji-100 Stearate 1 part by weight

Sesquioleate sorbitan 1.4 parts by weight

4 parts by weight of jojoba oil

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

2 parts by weight of macadamia oil

Tocopherol acetate 0.2 parts by weight

0.4 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methyl paraben 0.05 parts by weight

Glycerin 6 parts by weight

0.2 parts by weight of d-pandenol

0.2 parts by weight of triethanolamine

pt 41891 0.2 parts by weight

50.55 parts by weight of purified water

<1-2> Preparation of Lotion

0.1 parts by weight of the essential oil component of Dracosepalum poetidum, gourd extract, fractions thereof, or mixtures thereof of the present invention

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

Allantoin 0.1 part by weight

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

51.7 parts by weight of purified water

< Formulation example  2> Cosmetics  Preparation of Detergents in Compositions

<2-1> Preparation of soap

Dracocepalum poetidum essential oil component of the present invention, gourd extract, fractions thereof, or mixtures thereof 4.0 parts by weight

Soap base 94.25 parts

0.3 parts by weight of tocopherol acetate

Glycerin 0.2 parts by weight

0.8 parts by weight of PEG 4000 (polyethylene glycol 4000)

0.3 parts by weight of jojoba oil

Cetanol0.15 parts by weight

The components were mixed well in a mixer and then extruded in a soap making machine, cut and molded to a uniform size to prepare a soap composition. The soap base may be a sodium salt of palm oil, which is a natural oil commonly used in the art.

<2-2> body Cleanser  Produce

Dracocepalum poetidum essential oil component of the present invention, gourd extract, fractions thereof, or mixtures thereof 4.0 parts by weight

Ammonium Lauryl Sulfate 42 parts by weight

Cocamidopropyl Betaine 12 parts by weight

0.5 parts by weight of glycol distearate

Propylene glycol 1.0 parts by weight

A proper amount of pigment

Fragrance

Proper amount of citric acid

1.0 part by weight of drain (50% concentrate)

100 parts by weight of purified water is added.

Ammonium lauryl sulfate, cocamidopropyl betaine, glycol distearate, and propylene glycol were heated to 70 ° C. and emulsified by adding heated purified water to emulsify and cooled to room temperature to prepare a body cleanser.

< Formulation example  3> Preparation of the composition for oral cavity

<3-1> for oral cavity Gargle  Produce

Dracosepalum poetidum essential oil component of the present invention, gourd extract, fractions thereof, or mixtures thereof 0.1 to 1.0 g

Xyitol 5-10 g

Ethyl alcohol 5 ~ 15 g

Sorbitol 5-15 g

Saccharin Sodium 10-100 mg

Sodium Monofluorophosphate 500 ~ 1000 mg

Sodium Lauryl Sulfate 100-200 mg

Polysorbate 20 100 ~ 1000 ㎎

Peppermint Flavor 10-100 mg

Sodium benzoate 10 ~ 100 g

A proper amount of pigment

Purified water is added to make 100 g.

According to the above ingredients, according to the conventional method for preparing oral gargles, a gargle, which is an oral composition comprising the essential oil component of Dracosepalum poetidum, gourd extract or a fraction thereof, was prepared.

< Formulation example  4> For air cleaning  Manufacture of filter

It was dissolved in 5 wt% acetic acid aqueous solution of Dracosepalum poetitum essential oil component of the present invention, gourd extract, fractions thereof, or mixture mixture thereof so that the content ratio was 5 parts: 95 parts, respectively. The solution in which the Dracocepalum poetidium essential oil component, gourd extract, fractions thereof, or mixture mixture thereof was dissolved was extruded at a rate of 10 m / min into a platinum-gold alloy nozzle (0.1 mm φ × 300 hole) at room temperature. . The extruded Dracosepalum poetitum essential oil component, gourd extract, fractions thereof, or mixtures thereof were coagulated and solidified by neutralization in 5 wt% caustic soda.

Subsequently, the extruded Dracosepalum poetidium essential oil component, gourd extract, fractions thereof, or mixtures thereof are subjected to coagulation washing in a coagulation bath composed of water. Ficus extract, fractions thereof, or mixtures thereof were stretched in a boiling water bath consisting of water maintained at a temperature of 98 ° C. to obtain wet fibers. 500 g of the actual weight of the wet fibers chelate saturated copper ions. The obtained complex compound was cut and the fiber for filter was prepared by cutting the chelating Dracosepalum poetitum essential oil component, gourd extract, fractions thereof, or mixtures thereof.

Claims (12)

Dracocephalum foetidum ) Antimicrobial composition comprising the extract as an active ingredient. According to claim 1, wherein the Dracosepalum Poetidum extract is an antimicrobial composition, characterized in that the essential oil component of Dracosepalum Poetidum. According to claim 2, wherein the Dracosepalum Poetidum extract (a) extracting the dried Dracosepalum poetidum by distillation to obtain an extract; And (b) after the cooling of the extract comprises a Drakosepalum Poetidum extract, characterized in that the essential oil component of Dracosepalum Poetidum prepared by the method comprising the step of separating the essential oil portion of the upper layer as an active ingredient Antimicrobial composition. The antimicrobial composition according to any one of claims 1 to 3, wherein the antimicrobial composition is a general bacterium, a bacterium that has become resistant, a fungus and a fungus which has become resistant. The antimicrobial composition according to claim 1, wherein the composition is an external composition for skin. The antimicrobial composition according to claim 5, wherein the external composition for skin comprises a solution, a suspension, a spray, a patch, a pad, a cream, an ointment and a gel. The antimicrobial composition according to claim 5 or 6, wherein the external composition for skin is a cosmetic composition. The method of claim 7, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack Antibacterial composition comprising a liquid or solid soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser and bathing agent. According to claim 1, wherein the composition is an antimicrobial composition, characterized in that the composition for oral cavity. 10. The antimicrobial composition according to claim 9, wherein the oral composition comprises a toothpaste, a mouthwash and a mouthwash. The air-cleaning article comprising the Dracosepalum Poetidum extract of any one of claims 1 to 3 as an active ingredient. 12. The air cleaning article as recited in claim 11, wherein said air cleaning article comprises a filter for a fragrance, a deodorant, an air purifier, or a central air conditioner.