KR20080011535A - Cosmetic composition for protecting lip comprising ceramide and synthetic palmitoylpentapeptide as an active ingredient - Google Patents

Abstract

A cosmetic composition for protecting lip is provided to be able to significantly improve the lip moisturizing and lip wrinkle improving effects by comprising ceramide having excellent skin wall protecting and moisturizing effect and a conventional synthetic palmitoyl pentapeptide having the skin wrinkle formation inhibiting effect caused by natural aging in combination. A cosmetic composition for protecting lip comprises 0.1-10.0 wt.% of nano liposome stabilizing ceramide and synthetic palmitoyl pentapeptide by encapsulating the same, where the nano liposome has an average particle diameter of 10-100 nanometers, each content of the ceramide and the synthetic palmitoyl pentapeptide is 0.01-20.0 wt.% regarding the total weight of the nano liposome and the synthetic palmitoyl pentapeptide includes an amino acid sequence described as SEQ ID : NO. 1.

Description

Cosmetic composition for protecting lip containing ceramide and synthetic palmitoylpentapeptide as an active ingredient {Cosmetic Composition for Protecting lip Comprising Ceramide and Synthetic Palmitoylpentapeptide as an Active Ingredient}

The present invention relates to a cosmetic composition for protecting the lip containing ceramide and synthetic palmitoylpentapeptide as an active ingredient.

Lipstick is a lip cosmetic that is used for the purpose of protecting the lips while making it look beautiful by giving color effect to the lips.

Prerequisites for these lipsticks include: 1) non-irritating and harmless to the lips; 2) no unpleasant taste or smell; 3) smoothing, not seeping, and maintained for the required time; 4, storage Or keep the stick shape without being cut, deformed or softened during use. Fifth, there should be no change over time such as sweating and powdering. Sixth, the attractive appearance will be maintained and there will be no color change.

In addition, various studies have been conducted to improve the quality of lipstick. First, this study is to research the pigment dispersion base and to express the lips beautifully using highly purified pigments to express the beautiful lips by expressing the colors. Second, research is being conducted to protect the lips from external environmental influences. Third, research is being conducted on various substrates to make the lip line look beautiful without smearing or clumping.

The characteristics of the general lipstick formulation can be described as follows.

First, there are solid waxes that increase the hardness to maintain the appearance of the lipstick. Commonly used waxes include candelillanab, ceresin, carnauba wax, paraffin, wax, braze, beeswax, and microcrystalline lead, and if the solid content is high, the hardness of the lipstick becomes hard, hard, and used. The heavier the applicability, the lower the amount, the better the applicability, but the stability of the high temperature and less easily crushed, the amount of lipstick smeared on the lips are not evenly applied to the lip line spreading occurs.

Second, there is a liquid oil component having the largest proportion of the lipstick composition. These oils include castor oil, jojoba oil, squalane, lanolin, lanolin derivatives, oils derived from animals and plants, synthetic ester oils, silicone oils, and the like. It acts as an influential factor. For example, lanolin has excellent moisturizing properties and adhesion, which is commonly used in lipsticks, but lanolin itself is easily oxidized, which may cause discoloration and discoloration, burden on the lips, and allergy to lanolin has recently been reported. have.

Third, there is a pigment component that determines the color of the lipstick. These pigments are largely divided into organic pigments and inorganic pigments, and organic pigments generally have solubility. These dyes are rarely used in lipsticks because they may stain on the lips, but the colors of organic pigments vary. In order to make it brighter, the pigment | dye etc. which made these dyes into the insoluble lake are used. In addition, inorganic pigments such as iron oxide are being used, depending on the type and content of these pigments brings a lot of physical properties of the lipstick.

Fourth, it is made of various additives such as antioxidants, preservatives, fragrances, influence ingredients. In addition, a sunscreen and a special influence ingredient may be blended in order to protect the lips from ultraviolet rays and to prevent roughening.

In the case of the lips to which the lipstick is applied, the lips constantly contract and expand and move the most moving parts of the body, and they are always exposed to the outside, depending on environmental factors such as ultraviolet rays and pollutants in the air and dry climate. It is easily stimulated. Unlike the general skin structure, the skin structure of the lips has no sebaceous membrane and the stratum corneum is formed very thin, so there is absolutely no natural moisturizing factor and amount of water, and the rate of evaporation of water is extremely fast. Due to these characteristics, the lips are very weak in their defense against external stimuli and are severely damaged. Accordingly, it can be seen that the cosmetic for lips should have a more thorough protection and moisturizing effect unlike other cosmetics.

Until now, the following methods have been used to moisturize the lips with lipstick. The first is water or hydrophilic components. In particular, water should be particularly careful in the process of volatilization during the process, and if water is simply mixed for the moisturizing effect, water may be volatilized to promote dryness of the lips or shrinkage of the contents. .

Second, lanolin, lanolin derivatives, cholesterol derivatives, fatty acid and ester-based compositions including alcohol groups are used for lip protection and moisturizing purposes. However, as mentioned above, allergies have been reported in lanolin and lanolin derivatives, and are currently in limited use. In addition, in the case of cholesterol derivatives in consideration of the moisturizing effect 5% or more has a disadvantage in that the feeling of use is sticky and heavy.

As with the skin, wrinkles develop on the lips with age. This is due to structural changes deep within the skin that cause dermal degradation, ie loss of activity, within the skin. Therefore, not only wrinkles, but also lose their original firmness and gradually loosen the lips swelling occurs. Therefore, the general consumer wants to add the protection and moisturizing function for the lips and the function to improve the wrinkles on the commercially available lipstick.

Throughout this specification, numerous patent documents are referenced and their citations are shown in parentheses. The disclosure of the cited patent documents is incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

Accordingly, the present inventors earnestly researched to develop a substance that can protect the lips by maintaining the moisturizing of the lips and improving wrinkles. As a result, the nano-liposome is composed of ceramide having excellent skin barrier effect and synthetic palmitoylpentapeptide having excellent wrinkle improvement effect. The present invention was completed by confirming that lip moisturizing and wrinkle improvement effects are maximized by enveloping the lip penetration effect of the active ingredient.

Accordingly, it is an object of the present invention to provide a cosmetic composition for protecting the lips containing nanoliposomes stabilized by inclusion of ceramide and synthetic palmitoylpentapeptide as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to an aspect of the present invention, the present invention provides a cosmetic composition for protecting the lips containing nanoliposomes stabilized by containing ceramide and synthetic palmitoylpentapeptide.

The present inventors are conducting research to impart a moisturizing and wrinkle improvement effect to the cosmetic composition for protecting the lips, while encapsulating and stabilizing the synthetic palmitoylpentapeptide, which is effective for improving skin wrinkles and ceramides having excellent skin barrier protection, on nanoliposomes. It was confirmed that a clear lip moisturizing and lip wrinkle improvement effect was produced when the cosmetic was prepared.

Ceramide in the cosmetic composition of the present invention is one of the skin structural lipids, which is present in the stratum corneum of the skin epidermal layer and accounts for about 40-50% of the lipid component of keratinocytes. Such ceramide forms a multiple lamellar structure between keratinocytes along with other keratinocyte lipid components, thereby inhibiting evaporation from the skin and maintaining moisture to soften the skin and keep the skin healthy.

Meanwhile, the synthetic palmitoylpentapeptide included in the cosmetic composition of the present invention is a carboxyl terminal fragment of collagen type I, which is important for skin connective tissue and wrinkle formation and moisturizing, and forms collagen type I and type III in fibroblasts and It has been reported to promote the synthesis of fibronectin, which facilitates the communication between cells in relation to the cell-to-cell adhesion (Korean Patent Application No. 1999-55198). In addition, the inventors have found that synthetic palmitoylpentapeptide increases the biosynthesis of collagen IV, a component of dermal epidermal junction (DEJ).

Synthetic palmitoylpentapeptide has a structure (Pal-Lys-Thr-Thr-Lys-Ser) in which a palmitate group is linked to a pentapeptide (lysine-threonine-threonine-lysine-serine) consisting of five amino acids. Preferred synthetic palmitoylpeptide peptides of the present invention are peptides having the amino acid sequence set forth in SEQ ID NO: 1. Synthetic palmitoylpentapeptide can easily penetrate the epidermal layer of the skin by palmitate group, which is a lipophilic group, and penetrate the dermal layer easily by the amino acid group (pentapeptide), which is a hydrophilic group, and can effectively act on fibroblasts present in the dermal layer.

Regarding the ceramide and synthetic palmitoylpentapeptide used in the lip cosmetic composition of the present invention, the skin structure and components will be described in more detail as follows.

The skin consists of the epidermis, the dermis and the subcutaneous fat, which in turn consists of the stratum corneum, granule layer, pore layer and basal layer. The function of the stratum corneum, the uppermost layer of the skin barrier that acts as a protective barrier for the human body, (1) prevents drying of the epidermis by acting as a barrier that inhibits the loss of moisture and electrolyte out of the skin, and (2) the epidermis is normal biochemical metabolism. It provides an environment to protect the human body from (3) external physical damage and chemicals, and (4) prevents the invasion of bacteria, fungi and viruses into the skin.

The stratum corneum consists of keratinocytes and keratinocyte layers, and the keratinocyte lipids that play a crucial role in the role of the skin barrier are ceramide (45%), cholesterol (25%) and fatty acids (15%). It consists of.

Ceramide, which occupies the most part, is known to play an important role in maintaining skin barrier. Evidence that ceramides play an important role in skin barrier is as follows. (1) When breaking the skin barrier with acetone, the activity of serine-palmitoyl transferase (SPT), an enzyme involved in ceramide synthesis, increases, and then the synthesis of ceramide increases. At this time, pretreatment with β-chloroalanine, an irreversible inhibitor of SPT, causes a slow recovery of the skin barrier. (2) The amount of ceramide removed from the stratum corneum by the continuous treatment of the solvent is proportional to the degree of water loss on the epidermis. (3) Topical application of ceramide can restore damaged skin barriers. In addition, ceramide is responsible for the maintenance of moisture to prevent external leakage of moisture other than the skin barrier function to prevent the intrusion of external stimuli. When the amount of ceramide is reduced in the skin, the skin barrier is not complete and the skin becomes rough with the loss of moisture in the body.

Six kinds of ceramides (ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5, ceramide 6) are known to exist in the human body.

The ceramides used in the cosmetic composition for lip protection of the present invention are not limited to any kind, such as animal ceramides, synthetic ceramides, ceramides through yeast fermentation, and all are applicable.

The dermis is a connective tissue under the epidermis. The dermis is not as tightly packed with cells as the epidermis, but rather has a lot of extracellular space, part of which is filled by the network of macromolecules called extracellular matrix. In the dermis, there are histamine that causes an immediate allergic reaction, mast cells that produce serotonin (mast cells), and fibroblasts that synthesize and secrete an extracellular matrix. The components that make up the extracellular matrix are glycosaminoglycans or acidic mucopolysaccharides and fibrous proteins. There are several kinds of glycosaminoglycans, which differ in the type and number of sugar groups constituting the sugar, and the position and number of sulfate groups, and the skin contains a large amount of hyaluronic acid and dermatansulfate. These glycosaminoglycans are usually present in the form of proteoglycans in combination with proteins, and have gel structures that retain a large amount of water and are filled with fibrous proteins therein. Water in the gel acts to expand nutrients, metabolites and hormones from blood vessels to cells in tissues. Fibrous proteins include collagen, elastin and fibronectin and laminin, which are involved in adhesion with respect to structure. Collagen is the main protein of the extracellular matrix and works to maintain the shape of the tissue. Elastin is believed to contribute to the elasticity of the tissue by forming crosslinks with each other. Due to this structure, the dermis greatly affects the elasticity and tension of the skin.

In particular, collagen and glycosaminoglycans are substances contained in the dermal components of the skin and have a high binding force with moisture. Children's skin collagen has a high binding force with moisture, so fresh skin naturally has a large amount of moisture, and there is no tension and wrinkles.

Over time, the part of the cell that is conjugated to the extracellular matrix becomes weaker and the density of its structure decreases with the total number of cells interacting. The three-dimensional structure of the tissue is also gradually degraded and the ability of the fibroblasts to synthesize and secrete extracellular matrix is also reduced. In other words, the skin loses its elasticity.

Synthetic palmitoyl pentapeptide (Palmitoyl-Lysyl-Threonyl-Threonyl-Lysyl-Serine) used in the cosmetic composition for lip of the present invention stimulates the synthesis of collagen and glycosaminoglycans to promote elasticity and swelling of the dermis. Effectively enhance the characteristics.

Therefore, for the protection of the lips, a mixture of ceramide, which is effective for maintaining the skin barrier and moisture of the outermost stratum corneum, and synthetic palmitoylpentapeptide which stimulates the synthesis of collagen and glycosaminoglycans involved in elasticity in the dermis, The cosmetic composition for lips of the present invention is effective in improving lip wrinkles by imparting a moisturizing effect to the lips and gradually increasing elasticity.

In addition, the present inventors have studied that when the two components are used together, they are more effective in moisturizing and improving the wrinkles of the lips, and are easily infiltrated into the skin by incorporating nanoliposomes rather than directly prescribed in the lip cosmetic composition. Therefore, the effect of moisturizing and improving wrinkles was dramatically increased, which was effective for protecting the lips.

According to a preferred embodiment of the present invention, the content of ceramide is 0.01-20.0% by weight, more preferably 0.1-10.0% by weight, based on the total weight of nanoliposomes. In addition, the content of the synthetic palmitoylpentapeptide is 0.01-20.0% by weight, preferably 0.1-10.0% by weight relative to the total weight of the nanoliposome. If the content is less than the expected effect is difficult to appear, if the content is exceeded there is a problem in color and appearance in the cosmetic formulation. Synthetic palmitoylpentapeptide can easily penetrate the epidermal layer of the skin by the palmitoyl group (lipophilic group) and easily penetrate the dermal layer by the hydrophilic amino acid group, thereby effectively acting up to the dermal layer. Thus, even at very low concentrations, it promotes collagen synthesis and glycosaminoglycan synthesis very effectively.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 30-70 nm. When the average particle diameter of the nanoliposomes exceeds 70 nm, the improvement of lip penetration and the improvement of formulation stability are very weak among the technical effects to be achieved in the present invention.

According to a preferred embodiment of the present invention, the nanoliposomes of the present invention comprise a mixture comprising (i) a polyol, (ii) an oily component, (iii) a surfactant, (iii) a phospholipid, (iii) a fatty acid and (iii) water It is prepared by, the mixture is characterized in that it does not contain a monohydric alcohol.

The polyol used in the nanoliposome of the present invention is not particularly limited, preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xylitol, Sorbitol and mixtures thereof. It is preferably selected from the group consisting of propylene glycol, 1,3-butylene glycol, glycerin and mixtures thereof, most preferably glycerin. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposomes of the present invention may be various oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil; Synthetic synthetic oils; Silicone oils such as dimethicone and cyclomethicone series; Animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine; cholesterol; Cytosterol; Cholesteryl sulfate; One selected from the group consisting of _cytosteryl sulfate and C _10-40 fatty alcohol. The amount used is 2.0-30.0% by weight based on the total weight of nanoliposomes, preferably 3.0-20.0% by weight.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Anionic surfactants or alkoxylatedalkylethers, alkoxylatedalkylesters, alkylpolyglycosides, polyglyceryl esters and the like selected from the group consisting of alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates; Nonionic surfactants selected from the group consisting of sugar esters can be used, preferably alkyl lactylates belonging to the anionic surfactant or polysorbates belonging to the nonionic surfactant. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-3.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. In the preparation of the nanoliposomes of the present invention, the amount of lecithin is 0.5-10.0% by weight, preferably 2.0-6.0% by weight, based on the total weight of nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of C _12-22 alkyl chains, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. It is more preferably selected from the group consisting of stearic acid, palyoleic acid and mixtures thereof. In the preparation of the nanoliposomes of the present invention, the amount of fatty acid used is 0.05-3.0% by weight, preferably 0.1-1.0% by weight, based on the total weight of nanoliposomes.

The water used in the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 2.0-30% by weight, preferably 5.0-20.0% by weight based on the total weight of the nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The nanoliposomes thus prepared have the advantage of maximizing the skin penetration effect of the active ingredient, especially the lip penetration effect, by dissolving various types of poorly soluble substances and stabilizing unstable substances.

According to a preferred embodiment of the present invention, the content of the nanoliposome is 0.1-10.0% by weight, preferably 0.1-3.0% by weight relative to the total weight of the cosmetic composition for lips. In addition, when the content of the nanoliposome stabilized with the ceramide and the synthetic palmitoylpentapeptide is less than 0.01% by weight, no obvious lip moisturizing and antiwrinkle effect is expected, and when the content exceeds 10.0% by weight, the apparent effect is increased. The increase in is not expected and the feeling may become somewhat heavy.

Lip cosmetic composition of the present invention exhibits the use of lip protection through lip moisturizing enhancement and wrinkle improvement. Throughout the present specification, the term 'lip protection' means to protect the lips by improving the moisturizing of the lips and improving the condition of the lips through suppression of wrinkle formation of the lips and improvement of the generated wrinkles.

According to a preferred embodiment of the present invention, the lip cosmetics comprising a nanoliposome encapsulated with a mixture of ceramide and synthetic palmitoylpentapeptide have improved lip moisturization, improved lip roughness and lip wrinkles as compared to each of the above components and the mixture of the above ingredients. The improvement effect is excellent (reference: Experimental example 1 and table 3, experimental example 2 and table 3, experimental example 3 and table 5).

Ingredients included in the cosmetic composition for lip protection of the present invention as an active ingredient include ingredients commonly used in cosmetic compositions in addition to the active ingredients, for example, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings Adjuvants, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, but may be formulated with lip gloss, lip balm, lip cream, lipstick, and the like, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1 Preparation of Nanoliposomes Containing Ceramide and Synthetic Palmitoylpentapeptide

To prepare nanoliposomes, phase B was added in phase A with the composition shown in Table 1 below, followed by uniform wet, followed by addition of phase C, followed by heating and mixing to 70-80 ° C. The solution was passed through a high-pressure homogenizer (Microfluidizer M-110F, Microfluidics International Corporation) 1-5 times under conditions of 600-1200 bar and cooled to stabilize ceramide and synthetic palmitoylpentapeptide. Nanoliposomes were obtained.

Prize ingredient Content (% by weight) A glycerin to 100 B Hydrogenated lecithin 5.0 Ceramide 8.0 Caprylic / Capric Triglycerides 5.0 Macadamia Nut Oil 5.0 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 C Synthetic Palmitoylpentapeptide 10.0 Potassium hydroxide Quantity Purified water 10.0

Preparation Example 2 Preparation of Lipsticks Comprising Nanoliposomes Stabilized with Ceramide and Synthetic Palmitoylpentapeptide

Lipstick comprising nanoliposomes stabilized with ceramide and synthetic palmitoylpentapeptide of the present invention (Example) and lipstick containing ceramide and synthetic palmitoylpentapeptide (Comparative Example 1), lipstick containing no active ingredient (Comparative Example 2) was prepared in the following composition of Table 2.

Manufacturing process: Mix A phase uniformly at 85-95 degreeC. Add phase B to phase A and mix uniformly at 80 ° C. Add phase C and mix uniformly. D phase is mixed at 70 degrees C or less. After defoaming, phase E is mixed and then shaped into a lipstick.

Prize Ingredient Name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 A Castor Oil to 100.0 to 100.0 to 100.0 to 100.0 to 100.0 Polyethylene 2.0 2.0 2.0 2.0 2.0 Canterilla lead 5.0 5.0 5.0 5.0 5.0 Ceresin 8.0 8.0 8.0 8.0 8.0 B Octyldodecanol 10.0 10.0 10.0 10.0 10.0 Phenyltrimethicone 3.0 3.0 3.0 3.0 3.0 Hydrogenated Polyisobutene 5.0 5.0 5.0 5.0 5.0 Diisostearyl maleate 10.0 10.0 10.0 10.0 10.0 Polyglyceryl-2 Triisostearate 20.0 20.0 20.0 20.0 20.0 Caprylic / Capric Triglycerides 5.0 5.0 5.0 5.0 5.0 Tocopheryl Acetate 1.0 1.0 1.0 1.0 1.0 antiseptic a very small amount a very small amount a very small amount a very small amount a very small amount C Pigment 10.0 10.0 10.0 10.0 10.0 D Nanoliposomes (Manufacturing Example 1) 2.0 - - - - Ceramide - 0.16 - 0.16 - Synthetic Palmitoylpentapeptide - 0.20 - - 0.20 E incense a very small amount a very small amount a very small amount a very small amount a very small amount

In Table 2, the content unit is weight%.

Experimental Example 1 Comparison of Use Effect

Forty five females in their 20s and 40s were used to prepare lipsticks of Example 1, Comparative Example 1, and Comparative Example 2 each day for 4 weeks each day, and carefully observed lips before and after use. Evaluation was based on subjective judgment of the device and the subjects.

Clinical trial results Evaluation item Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Moisturizing effect 4.0 3.6 3.1 3.4 3.2 Lip Roughness Improvement 3.6 3.4 3.2 3.3 3.3 Lip wrinkle improvement 4.0 3.6 3.1 3.2 3.5 No lip skin irritation 3.6 3.5 3.5 3.5 3.5 Application 3.8 3.4 3.3 3.4 3.4 Average 3.80 3.50 3.24 3.36 3.38

Evaluation criteria: Very good-5, Good-4, Moderate-3, Somewhat dissatisfied-2, Very dissatisfied-1

When the results of the clinical trials of Table 3 above, it can be seen that the subjects' satisfaction with the lipstick of the Example was very high as compared with the comparative examples as a whole. In particular, there were significant score differences in moisturizing effect, lip roughness improvement, and lip wrinkle improvement.

Experimental Example 2 Moisture Effect Measurement (Corneometer)

In order to confirm the effect objectively with the subjective evaluation in the clinical experiment of Experimental Example 1 Corneometer CM820PC (Courage + Khazaka, Germany) and Tewameter TM 210 (Courage + Khazaka, Germany) was used to measure the moisturizing effect of the lips .

The corneometer was used to quantify the moisture content of the skin from 0 to 150 with the change of electrical conductivity according to the epidermal moisture content, and the Tewameter was used to measure the transdermal loss amount (TEWL), that is, the epidermal water loss.

Before applying the test substance, the electrical conductivity of the lips was measured, and lipsticks of Examples, Comparative Examples 1 and 2 were applied twice a day for 4 weeks, and then electrical conductivity and TEWL of the lips were measured after 2 and 4 weeks. .

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Electrical conductivity Before the test 40.86 40.78 40.73 40.74 40.80 after 2 weeks 47.36 43.19 41.20 42.10 42.50 4 weeks later 50.98 44.50 42.58 43.60 43.80 Water loss (g / ㎡ / h) Before the test 59.62 60.49 60.22 60.10 60.20 after 2 weeks 56.31 59.40 59.68 59.50 59.55 4 weeks later 51.87 56.65 60.19 56.80 56.90

Through the results of Table 4, it was objectively confirmed that the lipstick of the example in which nanoliposomes stabilized with ceramide and synthetic palmitoylpentapeptide were combined was superior to the comparative examples as in clinical trials.

Experimental Example 3 Wrinkle Improvement Effect

Wrinkle improvement effect was measured for the lip cosmetics containing nanoliposomes stabilized with the ceramide of the present invention and synthetic palmitoylpentapeptide.

Ten women in their 20s and 40s were selected, and the lip cosmetics of Examples and Comparative Examples 1 and 2 were applied twice a day for 0.2 weeks each for 4 weeks on an area of 2 × 2 cm 2 of the upper arm. Before and after 4 weeks of application with a silicone solution, a replica of the skin wrinkles was released, and the skin wrinkles were measured with a skin visiometer (Skin visiometer SV 400, Courage + Khazaka, Germany). The image of the replica was analyzed three-dimensionally with a CCD camera, and the wrinkles were improved by the average wrinkle roughness (Rz) of Equation 1, which is a value obtained by dividing the sum of roughness (Rm: m is an integer of 1 or more) of each wrinkle by the number of wrinkles. The effect was analyzed. The experimental results are shown in Table 5.

R _z = (R ₁ + R ₂ + R ₃ + ... _Rm-2 + R _m-1 + R _m ) / number of wrinkles (m)

Skin wrinkle improvement division ΔRz = Rz before use-Rz after 4 weeks Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Subject 1 0.038 0.031 0.005 0.018 0.020 Subject 2 0.021 0.044 0.002 0.010 0.022 Subject 3 0.089 0.062 0.011 0.033 0.049 Subject 4 0.070 0.058 0.005 0.028 0.042 Subject 5 0.094 0.090 0.008 0.060 0.076 Subject 6 0.058 0.025 0.003 0.018 0.020 Subject 7 0.106 0.067 0.009 0.054 0.060 Subject 8 0.122 0.103 0.010 0.080 0.092 Subject 9 0.043 0.050 0.004 0.031 0.041 Subject 10 0.067 0.033 0.008 0.025 0.030 Average 0.071 0.056 0.007 0.036 0.045

Through the results in Table 5, it was confirmed that the lip cosmetics of the embodiment containing the nanoliposomes stabilized ceramide and synthetic palmitoylpentapeptide showed the highest wrinkle improvement effect. In addition, the lip cosmetics of Comparative Example 2, which did not contain ceramide and synthetic palmitoylpentapeptide, showed little wrinkle improvement.

Hereinafter, on the basis of the results of the experimental example, a lip cosmetic composition containing nanoliposomes stabilized with the ceramide of the present invention and synthetic palmitoylpentapeptide is provided. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1. Lip Gloss

Prize Ingredient Name Content (% by weight) A Hydrogenated Polyisobutene to 100.0 Microcrystalline Lead 1.0 Candelilla lead 2.0 Petrolatum 10.0 Octyldodecanol 10.0 Phenyltrimethicone 3.0 Diisostearyl maleate 10.0 Polyglyceryl-2 Triisostearate 20.0 Tocopheryl Acetate 1.0 antiseptic a very small amount B Pigment 1.0 C Nanoliposomes (Manufacturing Example 1) 2.0 D incense a very small amount

Manufacturing process: A phase is weighed and mixed uniformly at 85 ° C. After adding B phase and disperse | distributing uniformly, it cools to 70 degreeC and inputs and mixes C phase. After defoaming, add phase D, mix for a short time, and fill the lip gloss container.

As described in detail above, the present invention provides a cosmetic composition for protecting the lip containing ceramide and synthetic palmitoylpentapeptide as an active ingredient. The cosmetic composition for lip protection of the present invention includes a ceramide having excellent skin barrier protection and moisturizing effect and a conventional synthetic palmitoylpentapeptide component having an effect of inhibiting skin wrinkle generation by natural aging, thereby improving lip moisturizing and lip wrinkle effect. Improves dramatically and is effective for protecting lips.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

<110> COREANA COSMETICS CO., LTD. <120> Cosmetic Composition for Protecting Lip Comprising Ceramide and          Synthetic Palmitoylpentapeptide as an Active Ingredient <130> dp-2006-0368 <160> 1 <170> KopatentIn 1.71 <210> 1 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> palmitoylpentapeptide <220> <221> LIPID <222> (1) <223> PALMITATE <400> 1 Lys Thr Thr Lys Ser   1 5

Claims (6)

Cosmetic composition for lip protection containing a nanoliposome stabilized by containing ceramide and synthetic palmitoylpentapeptide as an active ingredient. According to claim 1, wherein the content of the nano liposome is a cosmetic composition for protecting the lips, characterized in that 0.1-10.0% by weight based on the total weight of the cosmetic composition. According to claim 1, wherein the average particle diameter of the nanoliposomes is 10-100nm, preferably a cosmetic composition for protecting the lip, characterized in that 30-70nm. According to claim 1, wherein the content of the ceramide and synthetic palmitoylpentapeptide is a lip protective cosmetic composition, characterized in that 0.01 to 20.0% by weight relative to the total weight of the nano liposomes. According to claim 1, wherein the synthetic palmitoylpentapeptide is a cosmetic composition for protecting the lips, characterized in that having an amino acid sequence described in SEQ ID NO: 1. The cosmetic composition of claim 1, wherein the cosmetic composition has a formulation selected from the group consisting of lip gloss, lip balm, lip cream, and lipstick.