KR20080007293A - Cultivation method of sword bean sprouts by improving germination ration and growing ratio - Google Patents

Abstract

A method for cultivating a horse bean by water culture, and a method for cultivating kongnamul (bean sprouts) from a horse bean are provided to improve sprouting ratio and freshness. A method for cultivating a horse bean by water culture comprises the steps of dipping the seed of a horse bean in the purified water having pH of 4.0-6.0 at 24-29 deg.C for 6-12 hours; rotating the dipped one with 100-500 rpm for 20-60 min to remove the shell; dipping it in the purified water having pH of 4.0-6.0 at 24-29 deg.C for 4-10 hours to separate the shell from the seed fruit; seeding it on a cultivator having a punched hole of 5-10 mm with a density of 4-8/10 cm^2; and cultivating it by spraying purified water at 24-29 deg.C for 6-12 days.

Description

Cultivation method of sword bean sprouts by improving germination ration and growing ratio}

The present invention relates to a method of cultivating bean sprouts by hydroponic cultivation of soybean beans, which have not been used as bean sprouts despite their usefulness due to the remarkable delay of germination due to the rigidity of seedlings. It effectively breaks the dormancy of seeds and improves germination rate, while establishing suitable conditions for hydroponic cultivation of soybean seeds and spraying oxygen water with high oxygen content at regular intervals, so that vitamin A, C and urease, It relates to a method for cultivating bean sprouts sprouts containing a large amount of useful components such as Concanavalin A, niacin.

  It contains nutrients such as protein, starch, lipids, various minerals and vitamins. Especially, it contains a large amount of vitamin A and C which are not contained in other beans or trace amounts. Contains a lot. In particular, hemagglutinin isolated from soybeans contains a large amount of a protein called Concanavalin A, which is known to have detoxification, anti-tumor properties, strong division and inhibitory effects on transformed cells. Vitamin B3 is known to promote blood circulation, strengthen blood pressure, and reduce cholesterol. These bean beans have been used in Chinese medicine and high-grade cooking since ancient times, and are now known as folk remedies for purulent diseases such as sinusitis and hemorrhoids, and have been developed as beverages and health foods in Japan.

  However, bean beans, also called dodus, have been cultivated as herbal and functional weak beans on some farms and sold at high prices until recently. However, they are recognized as unfamiliar crops by consumers and farmers. In particular, the soybean seeds have thick seedlings and germination inhibitors, which are representative of the impervious seed seeds, which are characteristic of impermeable seedlings.The germination rate stays around 60% and the growth rate is within 10 days after planting. This is so low that the use of hydroponic cultivation technology such as peanut, black soybeans has been significantly increased, while hydroponic cultivation technology of soybeans has not been developed.

  The low germination and growth rate of the soybean seeds is due to the thick and the impervious material of the soybean seeds, and it is believed that the seedlings contain germination inhibitors such as dormant materials or dormant of the seed germ to suppress germination. Substances such as pectin in cells and suverin in pectin are presumed to be one of the causes, and conventionally, various treatments for the seeds are physically or chemically attempted to solve the poor germination caused by such causes. For example, a) a method of immersion in water for a long time, b) a method of mixing abrasive particles such as sand and seeds and rotating them in a drum, and c) peeling them under pressure by applying pressure to a temporarily frozen epidermis by liquid nitrogen or the like. Method d) A method of puncturing a part of the seed by irradiating a laser beam to a part of the seedling has been carried out. (Japanese Patent No. 2579822, Japanese Patent Application Laid-Open No. 64-75045, Japanese Patent Application Laid-Open No. 8-70625) Japanese Patent Application Laid-Open No. 8-70626, Japanese Patent Application Laid-open No. Hei 5-49309)

  However, each of these improvements has the following drawbacks.

In the method of immersing in water for a long time, it can be treated in large quantities, but long time immersion in water does not sufficiently peel the seedlings, so that the effect of improving germination rate is negligible. There is a problem that the seeds are rotted, and there is no solution to the problem of slow growth for a certain period after germination.

In the method of bleaching abrasive grains by mixing seeds and abrasive particles such as sand in the drum and rotating them in a drum, variation in germination may occur, resulting in a difference in germination rate, and deterioration of seeds due to frictional heat or physical impact. There is a concern and the method of rejuvenation alone has little effect on the improvement of germination rate and the same method as a) does not provide a solution to the problem of slow growth for a certain period after germination.

The method of peeling by applying pressure to the temporarily frozen capillary by the liquid nitrogen of c) has the greatest germination improvement effect among the proposed methods, but it is difficult to treat a large amount because the seed size is not uniform and the equipment and cost are high. There is a problem that arises and does not provide a method for improving the growth rate after germination in the same manner as a), b).

As the method of c), the method of puncturing the endothelial through the irradiation of the laser light of d) is difficult to mass-process the seed and requires an expensive laser irradiation device, and the punctured endothelial takes more than 72 hours to germinate and germinate. As a result, the germination rate improvement effect is insignificant, and there is no suggestion on how to increase the growth rate after germination.

  The present inventors recognize that most of the conventional methods for improving germination rate of hard seed have little effect on the improvement of germination rate, and some methods have high germination rate improvement effect, but it is difficult to incur high cost and mass treatment, and thus, it is difficult to reduce hard seeding at low cost. We developed a method to drastically improve the germination rate by avoiding a large amount and containing proper moisture for germination, and also improved the problem of slow productivity due to a slow growth rate for a certain period after germination, thereby cultivating bean sprouts in large quantities. We have developed a hydroponic method that can be used.

 An object of the present invention is to improve the germination rate of soybeans due to the thickness and impermeability of the seedlings, germination inhibitors, etc. and to obtain a uniform germination improvement effect at low cost in a method capable of cultivating soybean sprouts that have elongated their hypocotyls through hydroponic cultivation. It is possible to provide a hydroponic cultivation method of soybeans which can improve the growth rate after growing season.

  In view of the problems of the prior art, the present inventors first examined a method of physically wave treating a bean bean seedling to improve germination rate. However, the simple wave treatment method has a problem in that it is difficult to carry out mass processing mechanically due to the hardness of the seed, and the wave efficiency is low and uneven. Therefore, each seed coat has to be wave-treated. Seeds decayed according to pH, temperature, and soaking time, and the effect of soaking of seeds on the germination rate was also lower than that of the seed treated only by wort treatment. Particularly, the seeds decay during growth were excessive in water content due to pH and excessive soaking time. Is assumed to be affected. Therefore, in order to significantly improve the germination rate of the soybean seed, it is necessary to develop a method capable of containing adequate moisture while simultaneously peeling off the soybean seed of the soybean seed in large quantities efficiently.

  In addition, another problem of the soybean seed is that it takes a long time to fully grow after the germination of dormant is completely broken out. When watering for hydroponic cultivation, the water content becomes excessive depending on the frequency of watering and the growing temperature. The problem is that the seeds are corrupt. Therefore, for the cultivation of bean bean sprouts through hydroponic cultivation, the development of a method of improving the growth rate after germination and a method of preventing the decay of seeds should also be developed.

  Hereinafter, matters related to the implementation of the present invention will be described in detail by way of examples.

In the germination rate improvement method of the soybean seed of the present invention in order to break the dormancy of the soybean seed without damaging the internal seed cultivation and to enable the efficient peeling of the soybeans in the order of the first immersion, wave treatment, secondary immersion, aeration Developed a method for sowing in the cultivator after the treatment with the treatment process, and the optimum treatment conditions of each process were derived by verifying the effect of the change of treatment conditions on each step and the germination rate and soaking treatment in the above process. The germination rate and the germination rate of the seed treated only by the germination process was compared and the germination rate improvement method was completed.

In addition, we verified the growth rate according to cultivators of various sizes with different sizes and numbers of perforated holes to set the size of hydroponic cultivator, and treated distilled water with 20 ~ 100ppm to improve the growth rate after germination. By verifying the change of growth rate according to the spacing interval and cultivation temperature of oxygen water, the method of improving the growth rate of soybean seed using high oxygen water was completed.

(Example 1)

Germination Rate of Seeds after Wave Treatment after Primary Soaking

In order to investigate the increase in germination rate of seeds by artificial seedlings, 100 grains of commercially available soybean seeds were immersed in immersion water adjusted to pH 4.0 ~ 6.0 for 12 hours and then perforated with a diameter of 2 ~ 10㎜ on the inner surface. It was placed in a cylindrical longitudinal sheller having a diameter of 40cm and a height of 60cm with a perforated network made of SUS304 material having a thickness of 0.5 to 2.5 mm, and rotated at 200 rpm for 30 minutes for wave treatment. The seeded seedlings were placed on a Petri dish (φ12 × 3㎝) and placed on two sheets of filter paper (Whatman No. 1), and 50 ml of distilled water was added per Petri dish, and then sieved 10 grains. In order to prevent the loss of water, it was sealed with a Paraplast film and tested in a thermostat at 25 ± 1 ℃, and it was considered that germination was more than 2 mm.

Germination Rate of Seeds According to Whey Treatment (Unit:%, Germination) 1day 2day 3day 4day 5day 6day 7day Wave-free treatment zone 0 0 0 0 0 0 2 Wave treatment zone 0 0 13 28 58 84 98

As shown in Table 1, the soybean seed treated with the soybean showed a germination rate of 98% in 7 days after the tooth sowing soybean seed treatment had an effect of increasing the germination rate of the seed.

(Example 2)

Seed germination by temperature

In the same manner as in Example 1, 80 seeded soybean seeds after primary immersion were laid with two filter papers (Whatman No. 1) in a Petri dish (φ12 × 3㎝) and 50 ml of distilled water was injected per Petri dish. After that, 10 teeth were healed. In order to prevent the loss of water, it was sealed with a Paraplast film and incubated for 20 days at 20 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃ constant temperature for 7 days, it was considered that germination was elongated more than 2㎜.

Germination rate change according to temperature change (Unit:%, Germination) Temperature 1day 2day 3day 4day 5day 6day 7day 15 ℃ 0 0 0 4 19 43 72 20 ℃ 0 0 6 21 47 78 85 25 ℃ 0 0 13 28 58 84 98 30 ℃ 0 3 17 45 81 86 93

After the first immersion, the germination test after 7 days of germination in the incubator with different temperature after wave treatment on the seedlings showed that the germination rate was 72% at 15 ℃, 85% at 20 ℃, 96% at 25 ℃, and 30 It was 93% at ℃. There was no significant difference in germination rate after 7 days between 25 ℃ and 30 ℃, but at 30 ℃, some seeds began to rot with browning from 3 days after germination, and the temperature for germination of soybean seeds was 25 ± 1 ℃.

(Example 3)

Moisture Absorption Rate of Bean Seeds

Distilled water in which the seeded seeds were not waved and the seeded seeds were adjusted to 25 ± 1 ° C. in the same manner as in Example 1 to investigate the water absorption rate of the seedless and the soaked seeds. After soaking in water, the absorbance of the seeds was examined every 2 hours until 16 hours with the passage of time in a thermostat at the same temperature. Investigation of absorption was carried out by 20 tablets for each treatment group, dried at 28 ± 1 ℃ for 30 minutes in a dry oven, water was removed from the seedlings, weighed by digital balance, and the absorption rate was [(weight after absorption-weight before immersion) / before immersion. Weight] to 100.

Change of Water Absorption Rate According to Treatment Condition (Unit:%) division 2 hours 4 hours 6 hours 8 hours 10 hours 12 hours 14 hours 16 hours Wave-free treatment zone 4 10 15 18 26 32 35 38 Wave treatment zone 14 52 72 82 143 150 156 162

As a result of the water absorption of non-waved seeds and immersed seeds after wave treatment, the water absorption of primary immersed seeds without wave treatment was less than 20 even after 8 hours. However, after 12 hours, the water absorption rate increased to more than 30%, and the wrinkled seedlings were re-opened by absorbing water and separated from the seeds. Seeds immersed by wave treatment began to absorb water rapidly after only 2 hours and increased rapidly up to 10 hours after soaking, absorbing more than 140% of water, but after 10 hours, the increase in water absorption slowed down.

(Example 4)

Germination Rate According to pH of Soaked Water

To investigate the effect of pH of soaked water on the germination rate, 120 grains of unwavered soybeans were treated with distilled water, citric acid, Na2CO3, K2CO3, NaOH, and KOH. After soaking in a thermostat for 12 hours, two sheets of filter paper (Whatman No. 1) were placed in a Petri dish (φ12 × 3㎝), and 50 ml of distilled water was injected per Petri dish, followed by 20 grains. In order to prevent the loss of moisture, it was sealed with a Paraplast film and incubated in a thermostat at 25 ± 1 ℃ for 7 days, and it was considered that the roots were more than 2 mm long and germinated. Treated as.

Germination Rate According to pH Change of Soaked Water (Unit:%) Treatment pH4.0 pH5.5 pH6.0 pH7.0 pH8.0 pH9.0 pH10.0 pH11.0 pH12.0 Distilled water - 73 - - - - - - - Citric acid 74 79 - - - - - - - Na2CO3 - - - - 0% germination, all epithelial rot K2CO3 - - - - NaOH - - - - Germination more than 50%, but all seeds have a slight difference, but turn brown KOH - - - -

 Seeds cultured by immersion in pH-controlled immersion water treated with Na2CO3, K2CO3, NaOH, and KOH showed some differences, but all were decayed or turned brown, and there was no commerciality. The germination rate of seeds treated with was slightly higher, but there was no significant difference.

(Example 5)

Stripping Rate with Break Time

In order to investigate the effect on the peeling rate of seedlings according to the rupture time, 500 grains of soybean seeds were immersed in distilled water for 12 hours, and then perforated with a diameter of 5-7 mm and a perforated network made of SUS304 material having a thickness of 1.0-2.0 mm. After putting into 40 cm diameter and 60 cm high cylindrical longitudinal shredder, rotate 200 rpm each for 10 minutes, 20 minutes, 30 minutes, 40 minutes and 50 minutes for wave treatment, and then immerse in distilled water for 2 hours for 2 hours. Aeration at 100 liters / min was performed to check the peeling rate of the seedlings by using an air pump in a 5L reactor, and the germination part was completely peeled off, and even if peeled, the seed was separated. It was assumed that it did not come off.

Seeding rate according to rupture time (Unit:%) division 10 minutes 20 minutes 30 minutes 40 minutes 50 minutes Stripping Rate 32 58 72 65 47

Although the peeling rate increased up to 30 minutes as the time of breakup increased, the peeling rate did not reach 50% after 50 minutes due to the occurrence of many isolates.

(Example 6)

Seed growth rate with or without aeration process

In order to investigate whether the seed growth rate is increased by artificial seed removal, the experimental method is a 5-L reactor and the experimental section is first immersed in distilled water for 12 hours and then wave-shaped at 200 rpm in the seedling retarder of Example 5 for 30 minutes. After treatment, immerse for 2 hours for 10 hours, perform aeration for 60 minutes with the capacity of 100 liters / minute using an air pump, and the control section skipped the aeration process compared to the embodiment, and after 10 hours in a Petri dish (φ12 × 3㎝) Two sheets of filter paper (Whatman No. 1) were laid and 50 ml of distilled water was injected per Petri dish, and 15 pieces were dentified. In order to prevent the loss of water, it was sealed with a Paraplast film and tested in a thermostat at 25 ± 1 ℃. The elongation length was averaged by measuring the lengths of the remaining ones except the best and minimum lengths.

Growth rate of bean sprouts according to aeration process (Unit: ㎝, elongation length) 1day 2day 3day 4day 5day 6day 7day Airation 1.5 2.1 4.4 6.7 8.8 10.9 12.8 Non-airation - - 1.3 2.8 4.7 6.4 8.1

As shown in Table 6, the seed that did not undergo aeration process started to germinate on the third day after tooth growth and grew to 8.1 cm on the seventh day. At the beginning of the seventh day, it grew to 12.8 cm and showed a rapid growth rate.

(Example 7)

Freshness of Bean Sprouts According to Oxygen Concentration

120 grains of soybean seeds were immersed in distilled water for 12 hours, and then wave-treated at 200 ppm for 30 minutes using a seed germ liner and further immersed in distilled water for 10 hours. After dipping, aeration at 100ml / min for 60 minutes using air pump, dormant break and stripping treatment, put 2 sheets of filter paper (Whatman No.1) in Petri dish (φ12 × 3㎝) and per petri dish Distilled water, 20ppm, 40ppm, 60ppm, 80ppm, 100ppm was injected into the 50ml distilled water with dissolved oxygen controlled and 20 teeth each. In order to prevent the loss of water, it was sealed with a Paraplast film and tested for 7 days in a thermostat at 25 ± 1 ℃. The defect rate of bean sprouts was calculated as a percentage of browning phenomenon in the seeds.

Defective Rate of Bean Sprouts (Unit:%) division 1day 2day 3day 4day 5day 6day 7day Dissolved oxygen 20 ppm - - One 5 6 6 6 40 ppm - - - 2 2 4 5 60 ppm - - - 3 3 4 4 80 ppm - - - 2 2 3 4 100 ppm - - - One 3 3 3 Distilled water - - One 6 9 11 11

The decay rate of the incubated individuals was significantly decreased through the dissolved oxygen-enriched culture water compared to the incubated with distilled water. However, even if the dissolved oxygen is increased to more than 40ppm, the effect of reducing the defect rate is not remarkable.

(Test Example 1)

Analysis of General Ingredients of Bean Beans

For the analysis of the general components, the head, stem, and root of the soybean were divided and calculated by measuring the moisture content after drying at 105 ℃. As a total sugar, the reducing sugar was measured after acid hydrolysis, and the crude ash was measured by 550 ° C. direct ashing.

General Ingredients of Sprout Bean Herb (Unit:%, wet basis) Sample Moisture Total sugar Crude protein Crude fat Ash head 69.5 8.6 6.3 0.9 0.8 stem 73.6 6.7 1.2 0.4 1.2 Root 78.5 3.5 1.6 1.3 2.8

As shown in Table 3, moisture was 78.5% in the root portion, total sugar and crude protein in the head portion was 8.6%, 6.3%, crude fat was 1.3% in the root portion, respectively.

Test Example 2 Analysis of Inorganic Components of Soybean Herbs

Inorganic component analysis of each part of the soybean was added to 1 g of each sample and 25 ml of decomposition solution (HClO ₄ : H ₂ SO ₄ : H ₂ O ₂ = 9: 2: 5) was decomposed until it turned colorless on the hotplate and then 100 ml. After filtration by diagnosing, it was analyzed.

Inorganic Components of Sprout Bean Herb (Unit: ppm, wet basis) Sample Na M K Ca Mn Zn Cu Fe head 132.4 212.4 1423.5 612.2 4.8 3.3 0.7 14.1 stem 421.1 498.5 1769.2 1024.5 0.7 14.8 0.2 78.4 Root 612.4 9936.1 2598.3 769.3 26.1 22.6 20.1 1675.2

As shown in Table 4, the inorganic component content of each site was high in potassium, calcium, magnesium, and sodium, and a small amount of manganese, zinc, copper, and the like.

 (Test Example 3)

Compositional Amino Acid Analysis of Pea Herb

Constituent amino acid analysis for each of the soybean beans was analyzed by using an automatic amino acid analyzer to determine a certain amount of samples.

Amino Acid Constituents of Sprout Bean Herb (Unit: ㎎%) Composition head stem Root Aspartic acid 211 168 141 Threomine 183 53 72 Serine 168 75 88 Glutamic acid 321 124 110 Proline 167 110 ND ^{1 )} Glycine 118 62 68 Alanine 168 69 79 Cystine 21 14 16 Valine 201 72 80 Methionine 4 16 6 Isoleucine 180 59 17 Leucine 215 91 106 Tyrosine 107 28 32 Phenylalanine 231 81 79 Histidine 148 29 50 Lysine 216 309 119 Arginine 249 53 697 Total A.A. 2908 1413 1760 Total EAA ²⁾ 1378 710 529

¹⁾ Not deteted

²⁾ EAA; essential amino acid (Thr + Val + Met + IIe + Leu + Phe + His + Lys)

Amino acid is a factor that determines the quality of protein foods. As a result of analyzing the content of amino acids in each part of bean sprouts, as shown in Table 5, Glutamic acid of hair stem roots is 321 mg%, 124 mg% and 120 mg% The essential amino acid content was highest in the head.

 Despite its usefulness, plant resources with fruit seedlings, such as soybeans, have a low germination rate and high seed cost due to germination inhibitors, and have limited restrictions on their use as sprout-growing crops or herbicides. Therefore, in addition to the development of traditional foods such as soybean paste and cheonggukjang, their applicability to various fields such as beverages and health foods has been limited. In order to improve these problems, various methods have been proposed, but most of them require high cost and facility investment cost or have to process each seed individually, so they have not been linked to mass cultivation technology and can be easily applied to general growers such as using liquid nitrogen or laser light. No method has been developed.

   However, as described and demonstrated in detail above, the method proposed by the present invention is a method that can be relatively easily applied to the cultivated farms, and it is possible to drastically improve the germination rate and freshness of the seed without the enormous facility investment cost. It is a method that can be cultivated as a herb-type crop through hydroponic cultivation, the basic technology of crop cultivation. The bean sprout-type soybeans grown in the hypocotyl by applying the present method are rich in rutin, essential amino acids and various minerals, which are functional substances, as well as free sugars and fiber of monosaccharides. Therefore, the bean sprout-type bean beans cultivated by the method of the present invention create new demand as a functional food that uses the soybean bean having food and pharmacological value in a low cost and simple manner, and improve the added value of the bean bean and contribute to national health. And increase the income of pea farmers.

Hereinafter, the present invention will be described in more detail.

Specific contents of the present invention is the soaking step I for soaking the soybean seeds in purified water for 6 to 12 hours to maintain a temperature of 24 ~ 29 ℃ adjusted to pH 4.0 ~ 6.0; After immersion, it is perforated with a diameter of 2 ~ 10mm and attached to a 40cm diameter and 60cm high cylindrical longitudinal epidermis with a SUS304 material perforated network with a thickness of 0.5 ~ 2.5mm and rotated at 100 ~ 500rpm for 20 ~ 60 minutes. Wave treatment process for crushing; Immersion step II soaked again in the purified water maintaining the temperature of 24 ~ 29 ℃ adjusted to 4.0 ~ 6.0 to separate the seed of the seeded pea beans seed from the seed; Aeration process to abolish seed by aeration and bubbling the seed at a dose of 40-200 liters / minute using airpump for 20-80 minutes using airpump for seed removal of germinated soybean seed; Seeding process of seeding soybean seed peeled seedlings in a planter with 4 ~ 8/10 cm 2 perforated hole of the size of 5 ~ 10mm and dented in a dark room maintaining the temperature of 24 ~ 29 ℃; Oxygen water sprinkling process that promotes the growth of the soybeans by spraying the water of the soybean seeds at regular intervals three or more times per hour with water containing a high concentration of dissolved oxygen of 20 ~ 100ppm; After the seeding process is characterized by consisting of a process of collecting and washing the green bean sprouts grown to 5 ~ 15㎝.

As a prerequisite for the cultivation of sprouted bean sprouts, which have a lower hypocotyl germinated during the sprinkling process, should be grown in a cow at 24 to 30 ℃, the material of the growing machine should be made of plastic material. Germination in cows prevents chlorophyll formation in cotyledons and hypocotyls (stems), rapidly grows hypocotyls, while cotyledons do not develop, and plastic mats also sprout sprouts It is very important in the cultivation of the bean sprout type peas of the present invention since it does not collapse or stems, and avoids exposure from metal components that may elute from ordinary metals.

1 is a hydroponic cultivation process of bean sprouts and sprouts according to the present invention,

Figure 2 is a photograph four days after the growing phase of the bean sprouts cultivated through the first immersion, wave treatment, secondary immersion, sowing process in the present invention.

Figure 3 is a photograph after four days of growing phase of the bean sprouts cultivated through the first immersion, wave treatment, secondary immersion, aeration, sowing, oxygen water spraying process in the present invention.

Claims (3)

Soaking process I, which is soaked in soybean seed for 6 to 12 hours in a pH of 4.0 ~ 6.0 adjusted to maintain a temperature of 24 ~ 29 ℃; After immersion, it is perforated with a diameter of 2 ~ 10mm and attached to a 40cm diameter and 60cm high cylindrical longitudinal epidermis with a SUS304 material perforated network with a thickness of 0.5 ~ 2.5mm and rotated at 100 ~ 500rpm for 20 ~ 60 minutes. Wave treatment process for crushing; In order to separate the seedlings of the soybean seed from the seed, the soybean seeds which have undergone the soaking process II, which are immersed in purified water for 4-10 hours at a pH of 4.0-6.0 and maintained at a temperature of 24-29 ° C, have a length of 5-10 mm. Sowing in 4 ~ 8/10 / 10cm sized holes and planting them in the dark room maintaining the temperature of 24 ~ 29 ℃, cultivating purified water for 6 ~ 12 days while spraying purified water on the bean seeds at regular intervals 3 times or more How to Plant Hydroponic Seeds Soaking process I, which is soaked in soybean seed for 6 to 12 hours in a pH of 4.0 ~ 6.0 adjusted to maintain a temperature of 24 ~ 29 ℃; After immersion, it is perforated with a diameter of 2 ~ 10mm and attached to a 40cm diameter and 60cm high cylindrical longitudinal epidermis with a SUS304 material perforated network with a thickness of 0.5 ~ 2.5mm and rotated at 100 ~ 500rpm for 20 ~ 60 minutes. Wave treatment process for crushing; Immersion step II soaked again in the purified water maintaining the temperature of 24 ~ 29 ℃ adjusted to 4.0 ~ 6.0 to separate the seed of the seeded pea beans seed from the seed; In order to remove seed and promote germination of seeded soybean seed, aeration was carried out by airpump for 20 ~ 80 minutes with a capacity of 40 ~ 200 liters / minute and bubbling to abolish seed. After sowing the soybean seed that has been peeled from the seedlings, the soybean seed which has been sown in the dark room maintaining the temperature of 24 ~ 29 ℃ by sowing in 4 ~ 8 / 10cm2 perforated plant with 5 ~ 10mm sized holes. 6 ~ 10mm holes are planted in 4 ~ 8 / 10cm2 perforated plants and placed in a dark room that maintains the temperature of 24 ~ 29 ℃. How to cultivate soybean seed for 12 days Soaking process I, which is soaked in soybean seed for 6 to 12 hours in a pH of 4.0 ~ 6.0 adjusted to maintain a temperature of 24 ~ 29 ℃; After immersion, it is perforated with a diameter of 2 ~ 10mm and attached to a 40cm diameter and 60cm high cylindrical longitudinal epidermis with a SUS304 material perforated network with a thickness of 0.5 ~ 2.5mm and rotated at 100 ~ 500rpm for 20 ~ 60 minutes. Wave treatment process for crushing; Immersion step II soaked again in the purified water maintaining the temperature of 24 ~ 29 ℃ adjusted to 4.0 ~ 6.0 to separate the seed of the seeded pea beans seed from the seed; Aeration process to abolish seed by aeration and bubbling the seed at a dose of 40-200 liters / minute using airpump for 20-80 minutes using airpump for seed removal of germinated soybean seed; Seeding process of seeding soybean seed peeled seedlings in a planter with 4 ~ 8/10 cm 2 perforated hole of the size of 5 ~ 10mm and dented in a dark room maintaining the temperature of 24 ~ 29 ℃; Oxygen water sprinkling process that promotes the growth of soybeans by sprinkling oxygen water containing a high concentration of oxygen with a dissolved oxygen of 20 to 100ppm in the soybean seed at regular intervals three or more times an hour; Method of cultivating bean sprout sprouts by hydroponic cultivation, which collects and washes bean sprouts grown to 5 ~ 15㎝ after seeding process

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