KR20070017563A - Nanoliposome particles containing red ginseng irradiated with saponin, licorice, biotin, copper peptide and cytokine, cosmetics and soap compositions containing them - Google Patents

Abstract

The present invention relates to a red liposome particle containing red ginseng irradiated with saponin, licorice, biotin, copper peptide, cytokine, and cytokines, and a cosmetic composition containing the same. More specifically, the present invention is a shampoo, hair pack, hair conditioner, hair tonic, hair essence nanoparticles containing red ginseng irradiated with saponin, licorice, biotin, Cooper peptide, cytokine (Cytokines) It is characterized in that it is prepared by including in various formulations, such as cosmetic compositions and soaps, and has a remarkably excellent skin absorption ability to increase the blood flow in the scalp (promotes metabolism and cell regeneration, nourishment of hair cells), collagen biosynthesis performance and The present invention relates to a hair care cosmetic and a soap composition characterized by exhibiting an antioxidant and anti-inflammatory effect, thereby preventing hair loss and promoting hair growth.

Saturated Lecithin, Squalane, Red Ginseng Irradiated Phonin, Licorice, Cooper Peptide, Cytokine

Description

Nano-liposomal particles containing red ginseng irradiated with saponin, licorice, biotin, copper peptide and cytokines, and cosmetics and soap compositions containing them

The present invention relates to a red liposome particle containing red ginseng irradiated with saponin, licorice, biotin, copper peptide, cytokine, and cytokines, and a cosmetic composition containing the same.

More specifically, the present invention is a shampoo, rinse, hair pack, hair conditioner, hair tonic, nano liposome particles containing red ginseng irradiated with saponin and licorice, biotin, Cooper peptide, cytokines (Cytokines) It is manufactured by including it in cosmetic compositions and soaps of various formulations such as hair spray and hair essence, and has excellent skin absorption ability to increase skin blood flow in the scalp (promoting metabolism and regenerating cells, activating hair cell nutrition) and For free radical treatment, collagenase inhibitory effect, normalizing physiological condition by inhibiting testosterone effect on scalp tissue, improving hair condition and scalp, hair loss prevention and hair growth promoting effect It relates to cosmetics and soap compositions.

Normally, the cosmetic composition refers to a product that is applied to the skin for physiological activity and the skin has a very complicated structure.

Hair is made by changing the stratum corneum of the skin surface like nails, and is divided into hair shafts on the skin surface and hair roots in the epidermis.

Among the hair follicles in the lower part of the hair root, there is a capillary or a dermal papilla in which nerves are distributed. Hair does not continue to grow for life, but when the hair reaches the end of its life, it naturally falls out, and after a certain time, new hair grows from the pores again. The life of this hair shows the hair cycle leading to the growth phase, the regression phase, the resting phase, the hair loss, and the newborn hair.

The human hair cycle repeats over three to six years, and since each hair is independent, it grows and changes independently of any other hair. Because of this, hair loss is normal when human hair is normal.

The causes of excess hair loss include poor blood circulation, excessive male hormones, excessive secretion of sebum, hypoxia caused by dandruff and other bacteria, genetic factors, aging, and stress. The detailed reasons and mechanisms for early development are still unclear.

Alopecia includes alopecia areata, alopecia areata, female obesity alopecia, traumatic alopecia, inflammatory alopecia, and alopecia caused by tumors.In recent years, alopecia alopecia, male alopecia, and alopecia are caused by tumors, etc. The Korean population is decreasing in age and the female hair loss population is increasing.

Therefore, various hair loss prevention agents and hair growth agents have been developed and sold for the treatment of hair loss, but oral hair growth agents have problems of human side effects, and hair transplantation methods have problems such as cost and management methods, hair roots and hair follicles cannot be activated. , Formulas containing female hormones for suppressing male hormones or those containing minoxidil, which have been approved by the FDA, have short-term effects, but have the disadvantage of side effects and loss of effects when discontinued.

Accordingly, the present inventors have developed a composition containing red ginseng irradiated saponin, licorice, biotin, copper peptide, cytokine components, etc. in an optimal ratio capable of maximizing the efficacy and effect of each component after a long research result, preventing hair loss, hair growth, In addition to the effects of wool and anti-inflammatory, hair loss disease, one of the causes of dandruff, scalp, itching, drying and sebum overproduction prevention is proved excellent and no side effects. In addition, it proved that synergistic effect of the effect is obtained by promoting the skin absorption by nano liposomes of the five components.

An object of the present invention is to provide a nano-liposomal particles containing five components, such as red ginseng irradiated ponin, licorice, biotin, copper peptide, cytokine (Cytokines) and a cosmetic composition containing the same.

In order to achieve the above technical problem, the present invention realizes remarkably excellent skin absorption by effectively using the five components, and also maximizes the actual skin penetration effect by nano liposomes of these components, and skin blood flow in the scalp. Increasing (promoting metabolism and regenerating cells, activating nourishment of hair cells), inhibiting free radicals, inhibiting elastase, and inhibiting testosterone on scalp tissues to normalize physiological conditions and improve scalp and hair condition Of course, by achieving a very excellent cosmetics such as hair loss prevention effect and hair growth promoting effect to achieve the purpose.

The present invention provides nanoliposome particles containing five components, such as red ginseng irradiated with saponin, licorice, biotin, copper peptide, cytokine, and cytokines, and a cosmetic composition containing the same.

Hereinafter, each component contained in the composition of the present invention will be described in detail.

Red ginseng irradiated with the composition of the present invention is a glycoside component of ginsenoside and ginseng saponin pharmacological action among the various active ingredients of red ginseng has an excellent effect on the prevention of skin aging, prevention of hypertension or arteriosclerosis, hematopoiesis Action, anti-inflammatory, anti-allergic, anti-tumor action, protective ability against various stresses, increase the immune capacity, the adaptogen effect (adaptogen) effect, the antioxidant effect, cell regeneration effect, hair protection effect, blood circulation Numerous research papers and scientifically proven data on the effects of promoting action are available and have no side effects. Licorice (glycryrrhizaglabra root extract) is known as flavanone glycosides such as liqueurin, neoriquiritin, rhamoliquiritine and galcon glycosides such as isoliquiritine and neoisolacuiritin, and dozens of flavonoids There is a similar substance.

Licorice is an excellent antibacterial, anti-inflammatory, allergic, antiviral, and immunosuppressive effect. Glycirizine, the main component of licorice, has excellent skin soothing and anti-inflammatory effects. Biotin is also known as Vitamin H and Coenzyme R.

As a component of vios, a factor necessary for yeast growth, it plays an important role in the metabolism of carbohydrates, fats, and proteins in vivo, and is closely related to the process of cell development. In addition, biotin is ATP. When supplemented with coenzyme of four dependent carboxylase and deficient in biotin, the body's energy metabolism and various physiological functions are lowered and immune function is also lowered.

Copper peptides are peptides that bind copper ions to promote hair growth by increasing hair follicle cells and inhibit hair growth by inhibiting 5-alpha reductase in DHT (dihydrotestosterone- sebaceous glands and glands that inhibit hair growth). It prevents hair loss by preventing the production of secreted substances). Cooper ion also promotes the synthesis of ECM constituents by interacting with cell fiber bundles and increases the fiber bundle density (the amount of fiber) of the nipple to help regulate hair growth cycles.

Cytokines (b-FGF) are basic fibroblast growth factors and act as phase equilibrium genetic factors inside and outside the skin as promoters for skin regeneration.

It restores aging tissue and gives wound healing effect, promotes hair growth by increasing collagen and elastin synthesis, and activates hair cells.

Hereinafter, the present invention will be described in detail.

Increasing skin blood flow in the scalp (promoting metabolism and regenerating cells, activating hair cell nutrition) and inhibiting free radicals by using red ginseng irradiated with saponin and licorice, biotin, copper peptide, and cytokines Normalizes the physiological state by inhibiting the action, elastase inhibitory effect, and testosterone inhibitory effect on the scalp tissue, and improves the scalp and hair condition as well as prevents hair loss, promotes hair growth, and increases the antioxidant effect in the skin. In addition, by mixing the five components, saturated lecithin, glycerin and the like in an appropriate composition to stabilize the nano liposome particles, red ginseng irradiated with saponin, licorice, biotin, Copperpeptide, cytokine (Cytokines) components Skin absorption is improved.

Therefore, the cosmetic composition containing the nano liposome particles according to the present invention, the skin blood flow in the scalp (promotes metabolism and cell regeneration, activating hair follicle nutrient supply) and active oxygen inhibitory effect, collagenase inhibitory effect, scalp tissue In addition to normalizing the physiological state by the testosterone inhibitory effect, the scalp and hair condition are improved, as well as hair loss prevention and hair growth promoting effects, and also have anti-inflammatory and antioxidant properties.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples.

However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 preparation method of nano liposomes

First, 1.0 to 5.0% by weight of saturated lecithin, 1 to 10.0% by weight of glycerin, 1.0 to 10.0% by weight of squalane, 0.1 to 1.0% by weight of red ginseng saponinin, 0.1% to 1.0% of licorice, and 0.1% to biotin. 0.5% by weight, 1.0-10.0% by weight of cooper peptide, 1.0-10.0% by weight of cytokine were added, and purified water was added and mixed under stirring so that the total weight was 100% by weight.

The mixture was passed through a Micro Fluidizer (high pressure micro homogenizer) 3 to 5 times under a pressure of 600 to 1,200 bar and degassed to obtain nano liposome particles containing red ginseng irradiated ponin, licorice, biotin, cooper peptide, and cytokines.

TABLE 1

Composition of Nano Liposomes

The nano liposome of the present invention prepared by the composition as described above may contain both hydrophilic and lipophilic components in its structure and is a form suitable for effectively delivering the active ingredients to the skin.

Experimental Example 1: Skin Blood Flow Measurement Experiment

A blood flow meter (model name: moorLAB, UK) that can measure minute blood flow changes in biological tissues was confirmed to have an effect of improving blood flow by measuring blood flow volume of the scalp.

The sample was diluted 10% in distilled water and applied to the scalp, 5 minutes later, and compared with before use.

(Figure 1)

Blood flow increased by 93% compared to before use.

Experimental Example 2: Lipoxygenase Activity Inhibition Test

Inflammation-producing factors due to enzyme activity are the main causes of inflammation.

Experimental Example 2: Lipoxygenase Activity Inhibition Test

Inflammation-producing factors due to enzyme activity are the main causes of inflammation.

Enzyme solution and 0.5 ml of the sample were added to a sodium phosphate buffer solution containing 1.12 mM linolenic acid, and then reacted with Ttichloroacetic Acid and TBA reagent (Thiobarbituric Acid). The absorbance was measured at 535 nm.

(Figure 2)

For the experiment was prepared a cosmetic containing nano liposomes containing varying the test sample by content. The comparative sample adopts an antioxidant extract with antioxidant effect.

To test their antioxidant power, 8 grams of each cream is added 40 grams to 80% ethanol.

Extract for 1 hour while vortexing every 15 minutes.

The supernatant is taken after centrifugation for 5 min at 10000 rpm in a centrifuge.

Dilute 10 times with methanol

After dilution, centrifuge for 3 min at 10,000 rpm to avoid precipitation problems. After centrifugation, dispense into 96well as follows.

This is reacted for 20 minutes at room temperature and the absorbance is measured at 517 nm.

Calculate the scavenging activity (%) using the following formula

TABLE 2

Antioxidant inhibitory activity of the experimental group (%)

In the experimental results, in the experimental results of Experimental Example 3 and Comparative Example A, Experimental Example 4 and Comparative Example B, the antioxidant power of the nano liposome-containing cream of four mixtures other than red ginseng irradiated ponine was significantly higher than that of the gall bladder extract having excellent antioxidant power. Able to know.

Experimental Example 5 transdermal absorption effect experiment

TABLE 3

Composition of the formulations of Comparative Example and Experimental Example 5

The transdermal absorption test was conducted using the formulation of Comparative Example and the formulation of Experimental Example 5 prepared with the composition as shown in Table 3. Percutaneous absorption was divided into 1 cm ² area of hairless guinea pig skin, and each sample was treated in 0.5 gram basis weight so as not to be contaminated. After 30 minutes, 5 times were tested by tape stripping method. The concentration of red ginseng irradiated on the surface of the skin was analyzed after solvent treatment by HPLC. The results are shown in Table 4 below.

TABLE 4

Red Ginseng Irradiated Phosphorin Content (%)

As a result, in the formulation of the comparative example, red ginseng irradiated with the predominantly on the surface of the skin was found that almost no penetration of the skin, the formulation of Experiment 5 was found that most of the penetration into the skin.

That is, it was confirmed that the skin absorption of the red liposome particles of the present invention prepared by mixing red ginseng irradiated ponin and four components with saturated lecithin, glycerin and the like in an appropriate composition was significantly increased.

Experimental Example 6-9: Collagen synthesis effect experiment

Collagen biosynthesis experiments were performed to compare the anti-wrinkle effect indirectly.

This experiment was carried out selectively in a concentration range that is not toxic to cells in the actual cytotoxicity test.

For the isolation and culture of fibroblasts, the skin obtained from neonatal foreskin resection was removed with surgical scissors and skin sections 2-4 mm in size were made. Type I collagenase solution was added thereto and left at 37 ° C. for 12 hours to completely remove the collagenase solution. After removing the epidermis from the dermis, the dermis was finely chopped with a surgical knife, and then cultured fibroblasts. In this experiment, fibroblasts passaged 3-7 times were used.

The basic culture of human fibroblasts was 10% of glutamine (0.48 mg / ml), penicillin (100 IU / ml), streptomycin (50 μg / ml), and FBS (fetal bivine serum) with inactive complement for 30 minutes at 56 ° C. Dulbecco's modified Eagle's media (DMEM) from Gibco BRL (USA) added in proportions was used. 5 × 10 ⁵ fibroblasts were cultured per well in a 6-well multi chamber plate. Cells were grown to about 79-80% until sufficient culture. Each cell (red ginseng) irradiated with saponin, cytokine was treated with DMSO (dimethyl sulfoxide) to adjust the concentration, Ectoin was treated with distilled water to adjust the concentration. Each experimental condition was treated for each cell at different concentrations, and then incubated for 2 days. After 2 days, cell media and cells were collected and stored in a refrigerator.

Cell extracts were scraped with a small amount of PBS and then scraped with a cell scraper. 100 μl of 1N NaOH and 400 μl of distilled water were allowed to stand for one day, and a protein assay was performed. Media was used as a dye (dye) that can bind specifically to (soluble collagen) to the collagen assay (collagen assay) was used Sircol Collagen assay kit (bio color).

First, perform the experiment using 100 μl of 0.5 M acetic acid or acetate buffer solution, and then add each test sample in which the media was concentrated using standard collagen and amicon's centricon 10. Then, 1.0 ml sircol dye was added to each tube. The reagent was added and mixed for 30 minutes, the supernatant was removed by centrifugation at 5000 g for 10 minutes, and 1.0 ml Sircol Alkali Reagent was added thereto, and the mixture was mixed lightly and measured at 540 nm.

Again, the collagen assay value was corrected with the amount of protein. The experimental results were treated with only DMEM medium as a control group to evaluate the rate of collagen production increased compared to this control.

TABLE 5

Collagen biosynthesis rate (%) of each experimental group

From the results of the experiment, it was found that the collagen biosynthesis rate when mixed and used is significantly superior to the collagen biosynthesis rate when the red ginseng irradiated saponin and cytokine are used alone. That is, comparing the results of Experimental Example 6,7 and the results of Experimental Example 8,9, in the case of using a mixture of red ginseng irradiated saponin and cytokine, even if the effect is a small amount than when using one of the two components alone You can see that it is remarkable.

Experimental Example 10: Clinical test for skin irritation test and hair loss prevention, hair growth promoting effect and anti dandruff, itching prevention

Hair shampoo and hair essence composition containing red ginseng irradiated ponins, nano liposomes containing licorice, biotin, cooper peptide, cytokine, and hair shampoo composition for 30 men and women with severe hair loss nationwide. After applying the essence evenly over the scalp and massaging the hair, apply it to the hair for 4 months and observe the results every 2 months.

Of the 30 subjects, 20 were male and 10 were female and the mean age was 29.8 years.

TABLE 6

TABLE 7

86% of the subjects showed significant reduction of hair loss, 70% of hair growth and wool effect, 80% of subjects who felt dandruff, refreshing scalp, and strong hair strength. It was.

And 100% had no skin irritation or side effects for 4 months.

This is because the composition of the red ginseng irradiated saponinin of the present invention and nanoliposomal particles containing four components in shampoos and hair essences increases skin blood flow in the scalp (promotes metabolism and cell regeneration, nourishment of hair cells), collagen It has been shown to exert biosynthetic, antioxidant and anti-inflammatory effects, thus preventing hair loss and promoting hair growth.

In addition, it is considered that the composition of the present invention does not cause irritation or skin trouble even after long-term use.

Example 2 Preparation of Hair Shampoo Containing Nanoliposomal Particles

TABLE 8

Composition of Shampoo Formulations

Emulsification step of uniformly dissolving at a constant temperature (70 to 75 ℃) and homogeneously mixed in a general homo mixer using a component dissolved in oil as an oil phase component and a component dissolved in purified water as an aqueous phase component of Table 8 It was. After cooling the emulsified mixture, the perfume and nano liposome particles were added at about 40 ° C., sufficiently stirred, mixed, cooled, and defoamed to prepare a shampoo formulation as a cosmetic.

Example 3 Preparation of Hair Conditioner Containing Nanoliposomal Particles

TABLE 9

Composition of Hair Conditioner Formulations

The emulsification process of sufficiently dissolving uniformly at a constant temperature (70-75 ° C.) and homogeneous mixing at a predetermined temperature (70-75 ° C.) is carried out using the components dissolved in oil as the oil phase component and the components dissolved in purified water as the water phase component. It was. After cooling the emulsified mixture, the perfume and nano liposome particles were added at about 40 ° C., sufficiently stirred, mixed, cooled, and defoamed to prepare a hair conditioner formulation as a cosmetic.

Example 4 Preparation of Hair Mist Containing Nanoliposomal Particle Particles

TABLE 10

Composition of Hair Essence Formulations

The ingredients in Table 10 were sequentially added to the purified water, stirred, and nanoliposome particles were added to prepare a hair essence as a cosmetic in suspension.

Example 5 Preparation of Hair Tonic Containing Nano Liposomal Particles

TABLE 11

Composition of Hair Tonic Formulations

The ingredients in Table 11 were sequentially added to the purified water, stirred, and nanoliposome particles were added to prepare hair tonic as a cosmetic in suspension.

Example 6 Preparation of Soaps Containing Nanoliposomal Particles

TABLE 12

Composition of Soap Formulations

The components of Table 12 were stirred at 45 ° C. for the oil phase and the water phase, and then added to the nano liposome particles to prepare a soap after cooling and ripening.

In the present invention, characterized in that the nano-liposomal particles containing red ginseng irradiated with saponin, licorice, biotin (Copper peptide), cytokines (Cytokines) to the shampoo, hair essence formulations, It has remarkably excellent skin absorption, promotes cell metabolism in the scalp and regenerates the cells, collagen biosynthesis, activates hair cell nutrition and inhibits free radicals, and normalizes the physiological condition by inhibiting male hormones on the scalp tissue. As well as the improvement of the hair condition has excellent characteristics of exhibiting the effect of preventing hair loss and promoting hair growth, it is a very useful invention in the cosmetic manufacturing and beauty industry.

Claims (3)

Saturated lecithin 1.0 to 5.0%, glycerin 1.0 to 10.0% by weight, squalane 1.0 to 10.0% by weight, red ginseng checkonin 0.10 to 1.0% by weight, licorice 0.1 to 5.0% by weight, biotin 0.1 to 1.0% by weight, copper peptide 1.0 to 10.0 A nano liposome particle comprising by weight%, cytokine 1.0 to 10.0% by weight and the remaining amount of purified water. A cosmetic composition comprising 1.0 to 20.0% by weight of the nanoliposome particles of claim 1. The nanoliposome particles of claim 1, which contain red ginseng irradiated saponin, nano liposomes including licorice, biotin, cooper peptide, and cytokines to improve scalp and hair condition as well as prevent hair loss and promote hair growth. Compositions and Soaps