CN115554380B

CN115554380B - Composition for preventing and treating alopecia by acting on hair follicle tissue and application thereof

Info

Publication number: CN115554380B
Application number: CN202210663529.6A
Authority: CN
Inventors: 刘小溪; 乐艳; 陈露露
Original assignee: Haoxi Hainan Biotechnology Co ltd
Current assignee: Haoxi Hainan Biotechnology Co ltd
Priority date: 2022-06-13
Filing date: 2022-06-13
Publication date: 2023-09-26
Anticipated expiration: 2042-06-13
Also published as: CN115554380A

Abstract

The application belongs to the field of medical cosmetics, and in particular relates to a composition for preventing and treating alopecia by acting on hair follicle tissues and application thereof. The composition comprises black mulberry root extract, ginger extract and astragalus root extract. The application also provides application of the composition in preparing a product for improving the tissue activity of hair follicles, and the product is directly smeared or sprayed on scalp to promote telogen hair follicles to enter a regeneration period and promote hair growth. The application also provides application of the composition in preparing a medicament for improving the activity of hair follicle tissues cultured in vitro, and the composition is prepared into a tissue culture solution which acts on the hair follicle tissues cultured in vitro to improve the activity of the hair follicle tissues cultured in vitro, so that the survival rate of transplanted hair follicles is obviously improved.

Description

Composition for preventing and treating alopecia by acting on hair follicle tissue and application thereof

Technical Field

The application belongs to the field of medical cosmetics, and in particular relates to a composition for preventing and treating alopecia by acting on hair follicle tissues and application thereof.

Background

Hair, as part of the body structure, plays an important role in our daily lives. Firstly, the hair has a direct protection function on the scalp, and external force acts on the scalp to play a role of buffering, reduce damage and protect the head. Clinically, because hair exists, foreign objects cannot directly contact scalp except hair dye, contact with dust and bacteria is avoided, and therefore contact dermatitis of scalp parts occurs less than other parts. At the same time, the hair has very important aesthetic functions and unique and important psychosocial functions. The traditional Chinese medicine considers that hair is blood, kidney stores essence, hair grows in the hair, the hair depends on the enrichment of essence and blood, the dense and bright hair represents health, good images can be given to people, the shapes and states of the hair are different, the impressions of the people are different, and healthy and beautiful hair is more confident in interpersonal interaction. Also various hair diseases such as alopecia, small hair, dandruff, etc. can cause bad psychological effects such as spelt, anxiety, etc., serious persons even affect social activities, and thus care of hair has to be taken in daily life.

The hair which is normally shed is the hair in the withdrawal phase and the resting phase, and the hair which enters the withdrawal phase and the hair which newly enters the growing phase are in dynamic balance continuously, so that the normal quantity of hair can be maintained. Pathologically abnormal hair loss, such as male baldness or female hair line posterior movement, is a long-term resting period of hair follicles under the action of certain factors, and cannot enter the growth cycle. With the increase of social competition, the increase of survival pressure, the reduction of high fat diet, sleep, environmental impact and the use of hair styling products such as hair dyeing and perming, the hair loss patient has signs of younger hair loss.

At present, the common hair restorer in the market of China mainly comprises various hair restoring shampoos, health care products, washes prepared by decocting traditional Chinese medicines and the like, and mainly achieves the effect of promoting hair restoration by improving microcirculation, but the effect is generally poor. There are few drugs for treating pathological alopecia clinically, and these drugs have effects only on a small proportion of patients, and there may be some toxic and side effects in long-term use. Although hair follicle tissue transplantation can fundamentally solve the problem of alopecia, it also has the problems of extremely high price and low survival rate of hair follicle tissue. Thus, there is a great need for products that are effective in preventing hair loss and improving the survival of hair follicles during hair follicle transplantation procedures, yet are safe.

Along with the discovery of social economy, the natural green component products are favored by more and more consumers, and the traditional medicine is profound in combination with the Chinese herbal medicine resources in China, so that the natural green component products have great advantages for developing herbal plant anti-hair loss hair growing products. The theory of traditional Chinese medicine considers that the external treatment is the internal treatment, and the external treatment is the internal treatment, so the traditional Chinese medicine formulas for oral administration can be externally used. The research at present finds that the Chinese herbal medicines related to hair loss prevention, hair growth and development recorded in the medical books are mainly Chinese herbal medicines with the effects of promoting scalp blood circulation, improving hair follicle growth and development and inhibiting bacteria. The development of herbal plant hair-loss prevention and growth development products with safety, stability and strong effect is favored by consumers by combining modern science with Chinese ancient medical research.

For example, U.S. cleaning company, KYOWA company, japan, and Korean CROIX company all have patented application of a washing and caring product containing green tea extract for preventing hair loss and promoting hair growth. The Inaoka corporation of Japan researches the methanol extracts of 80 single Chinese herbal medicines for the shaved mouse backs, and discovers that the wild hawthorn, glossy privet fruit, polyporus, bletilla striata and the like have the strongest promotion effect on the growth of the mouse hairs, however, the single Chinese herbal medicine has limited promotion effect on the aspect of in vitro hair growth culture, and the Chinese herbal medicine compound mode is effective according to the complementary theory of Chinese medicine. The compounding of the anti-drop effect of the Chinese herbal medicines is regular. Researches show that the medicines with warm or cold nature are mainly used in the hair-growing prescription, and the medicines are mainly classified into four channels of liver, lung, spleen and stomach, and mainly have the effects of clearing heat and eliminating dampness, strengthening spleen and regulating qi, which also indicates the direction for the researches of the inventor.

The inventor of the present application has focused on research on natural products and their extracts related to individual prevention and treatment of hair loss and improvement of hair follicle tissue survival for many years, and unexpectedly found that combining the primary active ingredient black mulberry root extract and ginger extract with the auxiliary ingredient astragalus root extract in a specific ratio during the research process can significantly improve the effects of the primary active ingredient black mulberry root extract and ginger extract, thereby developing a composition product with simple ingredients, safety and effectiveness for preventing and treating hair loss by acting on hair follicle tissue. In particular, there has been no report heretofore on a composition product for preventing and treating alopecia and improving hair follicle tissue comprising an extract of black mulberry root.

Disclosure of Invention

The application aims to solve the defects of the prior art, realizes the development and utilization of natural products by adopting a modern research method, combines a large number of experimental screening, and provides the application of a composition prepared by combining a main active ingredient of black mulberry root extract, a ginger extract and an auxiliary ingredient of astragalus root extract in a specific proportion in the preparation of products for preventing and treating alopecia and improving the survival of hair follicle tissues.

Specifically, the application is realized through the following technical schemes:

in a first aspect, the present application provides a composition for preventing and treating alopecia by acting on hair follicle tissue, the composition consisting of black mulberry root extract, ginger extract and astragalus root extract, wherein the ratio of black mulberry root extract, ginger extract and astragalus root extract is (1-10) in parts by weight: (1-10): (0.5-3).

Alternatively, in the above composition, the ratio of black mulberry root extract, ginger extract and astragalus root extract is 5:4:1.

alternatively, in the above composition, the hair loss is one or more of androgenic hair loss, nervous hair loss, endocrine hair loss, nutritional hair loss, physical hair loss, chemical hair loss, infectious hair loss, congenital hair loss, and seasonal hair loss.

Preferably, the hair loss is androgenetic hair loss.

Alternatively, in the above composition, the black mulberry root extract is selected from one or more of the following: mulberone C, mulberone G, mulberone F or mulberone M.

The ginger extract is selected from one or more of the following: gingerol, gingerol or curcumin.

The gingerol is selected from one or more of the following: 6-gingerol, 8-gingerol or 10-gingerol.

The astragalus root extract is selected from one or more of the following: astragaloside IV or Astragalus polysaccharides.

More preferably, the composition consists of sanguisorbanone C, 8-gingerol and astragaloside IV.

In a second aspect, the present application provides a composition for improving hair follicle tissue activity, the composition comprising black mulberry root extract, ginger extract and astragalus root extract, wherein the ratio of black mulberry root extract, ginger extract and astragalus root extract is (1-10) in parts by weight: (1-10): (0.5-3).

alternatively, in the above composition, the composition promotes hair follicle in telogen to enter a regenerative cycle, promoting hair growth.

In a third aspect, the present application provides a composition for enhancing the activity of hair follicle tissue cultured in vitro, the composition consisting of black mulberry root extract, ginger extract and astragalus root extract, wherein the ratio of black mulberry root extract, ginger extract and astragalus root extract in parts by weight is (1-10): (1-10): (0.5-3).

alternatively, in the above composition, the composition improves microenvironment of hair follicle tissue, promotes transformation of telogen and catagen state hair follicle tissue into long-term hair follicle tissue, thereby increasing activity of the cultured hair follicle tissue and significantly increasing survival rate of transplanted hair follicle.

In a third aspect, the present application provides the use of a composition as described in the first aspect above or a composition as described in the second aspect above for the preparation of a product for controlling hair loss by acting on hair follicle tissue.

Alternatively, in the above-mentioned use, the product is a topical product.

More preferably, the product is a pharmaceutical or cosmetic product.

More preferably, the topical product is a gel, ointment, cream, foam, shampoo, conditioner or spray-type liquid formulation.

Alternatively, the product comprises a hair follicle tissue culture fluid that is commonly used clinically.

In the application, the black mulberry root extract and the ginger extract are main active components, and the astragalus root extract is an auxiliary component.

"adjunct ingredient" generally refers to a substance that has no or little target activity, but is capable of enhancing the target activity of the primary active ingredient. The target activity of the application is mainly to prevent and treat alopecia by acting on hair follicle tissues, improve the activity of cultured hair follicle tissues and improve the survival rate of transplanted hair follicles.

It is understood that within the scope of the present application, the above-described technical features of the present application and technical features specifically described below (e.g., in the examples) may be combined with each other to constitute new or preferred technical solutions. Is limited to a space and will not be described in detail herein.

Compared with the prior art, the application has the following beneficial effects:

the inventor surprisingly found that combining the main active ingredients black mulberry root extract, ginger extract and auxiliary ingredient astragalus root extract in specific proportions can significantly improve the effects of the main active ingredients black mulberry root extract, ginger extract and significantly reduce the dosage of the active ingredients, thereby developing a composition product which has simple ingredients, is safe and effective and can prevent and treat alopecia by acting on hair follicle tissues.

Experimental results show that the combination of the astragalus root extract (especially astragaloside IV) and the black mulberry root extract (especially sanggenon C) and the ginger extract (especially 8-gingerol) plays a synergistic role in preventing and treating alopecia and stimulating hair regeneration. In addition, the composition of the present application has a promoting effect on hair growth of hair follicle of mice cultured in vitro, and can promote proliferation of hair follicle bulb cells, and the effect is significantly superior to that of a composition containing only both black mulberry root extract (especially sanggenon C) and ginger extract (especially 8-gingerol).

The natural product extract and the natural product monomer thereof used in the composition can be extracted and separated from the plants such as the mulberry bark, the ginger, the astragalus and the like containing the active ingredients, and the composition has the advantages of wide sources, low cost, economy and high safety.

Detailed Description

The following description of the preferred embodiments of the present application is provided for the purpose of illustration and explanation only and is not intended to limit the present application. The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications. The reagents or equipment used were conventional products available for purchase through regular channels, with no manufacturer noted.

As used herein, the "black mulberry (MORUS NIGRA) root extract" is an antioxidant, anti-inflammatory agent and skin conditioner commonly used in the cosmetic field, and the black mulberry root extract contains reducing sugar, pentose and galactose anhydride, tannins, flavonoids, and the like. Can inhibit elastase and has antiaging effect; promoting synthesis of fiber bud cell brain amide, and softening skin; can well prevent and treat acne caused by high androgen; has effect in contracting collagen fiber, and can be used for slimming; has promoting effect on activation of luciferase, and has antiinflammatory effect; can inhibit tyrosinase activity, and has skin whitening effect.

The specific natural products of the black mulberry root extract used in the application, such as monomer sanguisorbanone C, sanguisorbanone G, sanguisorbanone F, or sancinin M, can be extracted and separated from plants such as mulberry bark containing the active components by adopting a conventional extraction and purification method in the field of natural pharmaceutical chemistry, and can also be purchased from commercial products.

As used herein, the "ginger extract" is a skin conditioner, an anti-inflammatory agent and an antioxidant commonly used in the cosmetic field, and the ginger extract has a certain inhibitory effect on dermatophytes, streptococci albicans and oral pathogens, but has poor inhibitory effect on common mold and the like; can enhance skin activity, activate skin, and has antioxidant and antiaging effects; can also promote hair growth, and can be used as hair promoter; it also has effects in keeping moisture and inhibiting odor.

As used herein, "ginger extract", "gingerol" or "curcumin" and the like of the present application can be extracted and isolated from plants such as ginger containing the active ingredient by a biological purification method, and can also be purchased from commercial products.

As used herein, the "astragalus membranaceus (ASTRAGALUS MEMBRANACEUS) root extract" is an antioxidant and skin conditioner commonly used in the cosmetic field, which is derived from the root of astragalus membranaceus, contains an important active ingredient of astragaloside, and can stimulate proliferation of cortical cells, and has the effect of conditioning skin. Has effects of resisting aging and preventing wrinkle.

The specific natural product monomer astragaloside IV or astragalus polysaccharide and the like in the astragalus root extract used in the application can be extracted and separated from plants such as astragalus containing the active ingredients by adopting a conventional extraction and purification method in the field of natural medicinal chemistry, and can also be purchased from commercial products.

Example 1: the composition has the effect of promoting hair growth on androgen alopecia model mice

1. Experimental grouping and administration

Male C57BL/6J mice of 6-8 weeks of age were randomly grouped, and were grouped together into the following 5 groups.

(1) Blank (0.2 ml of a conventional blank hair care base applied; n=10);

(2) Model group (0.2 ml of a conventional blank hair care essence base applied; n=10);

(3) Morgandone C+8-gingerol group (0.2 mL of hair care essence matrix containing Morgandone C and 8-gingerol is smeared, wherein the content of Morgandone C accounts for 5% of the total weight of the hair care essence matrix, the content of 8-gingerol accounts for 4% of the total weight of the hair care essence matrix; n=10);

(4) Astragaloside IV (0.2 mL of hair care essence matrix containing Astragaloside IV is smeared, wherein the content of Astragaloside IV accounts for 1% of the total weight of the hair care essence matrix; n=10);

(5) Morgandone C+8-gingerol+astragaloside IV (0.2 mL of a hair care essence matrix containing Morgandone C, 8-gingerol and astragaloside IV is smeared, wherein the content of Morgandone C accounts for 5% of the total weight of the hair care essence matrix, the content of 8-gingerol accounts for 4% of the total weight of the hair care essence matrix, the content of astragaloside IV accounts for 1% of the total weight of the hair care essence matrix, and n=10);

each group was given the product after 24h of dehairing on the back of the mice, 1 time/day. Groups (2) - (5) were reapplied after each day with 5mg/mL testosterone dilutions for 2 hours.

Preparation of C57BL/6J mice androgenetic alopecia model

The back hair of each group of mice was shaved with a shaver to an area of about 2cm×2cm, and then the remaining hair was removed by applying depilatory cream (Milting and depilatory cream) respectively according to the instructions, and it was confirmed that the current mouse hair growth was in resting stage (skin appeared pink). After dehairing for 24 hours, all groups of mice except blank groups are smeared with 5mg/mL testosterone diluent solution 0.2 mL/piece on the dehairing area of the back, 1 time/day, and continuous moulding is carried out for 28 days. The total period of the experiment is 4 weeks, and the mice normally ingest drinking water during the experiment.

3. Detection index and statistical method

The product was administered for 28 days, 1 time/day continuously. The skin color change of the mice was observed daily before the application of the samples, and the hair growth of the hair-free areas of the mice was photographed every 7 days and the percentage of hair growth was calculated.

Percent (%) of hair growth= (area of post-shave hair growth area/area of pre-shave hair area) ×100%.

Statistical data are expressed as mean ± standard error. Statistical analysis was performed using repeated-assay analysis of variance and the differences between groups were compared using Fisher LSD test.

4. Experimental results

The experimental results of hair growth in androgenic alopecia mice are shown in table 1 below.

Table 1: effect of the inventive composition on the Hair growth of androgen alopecia model mice

Note that: * P is:<0.01, compared to the blank; ^## ，p<0.01, compared to the model set; ^△△ ，p<0.01 compared with Morgandone C+8-gingerol group

As shown in Table 1, at day 1, hair growth did not begin in each group of dehaired C57BL/6J mice, and therefore the hair coverage was 0% in each group of experimental animals.

At two detection time points of day 21 and day 28, the hair coverage of the experimental animals of the model group is 10.1% and 21.5%, respectively, and the experimental results are significantly lower than that of the blank control group (p < 0.01), and the results show that the modeling of the androgenic alopecia mice model is successful.

For the sanggenon c+8-gingerol group, the hair coverage of the experimental animals was 13.6% and 29.3% higher than that of the model group at the two detection time points of day 21 and day 28, respectively, but the above results were not significant compared with the model group. The results show that the combination of sanggenon C and 8-gingerol has a certain effect of improving the hair growth of mice with androgenic alopecia models, but the effect is limited.

For the astragaloside group, the hair coverage of the experimental animals was 10.5% and 23.4% at the two detection time points on day 21 and day 28, respectively, close to the model group. The above results indicate that astragaloside alone has no effect on hair growth in androgen alopecia model mice.

For the sanggenon c+8-gingerol+astragaloside group, the hair coverage of the experimental animals was 35.4% and 66.5% at the two detection time points of day 21 and day 28, respectively, the result was significantly better than that of the model group (p < 0.01), and the result was significantly better than that of the sanggenon c+8-gingerol group (p < 0.01). The results show that the combination of sanggenon C+8-gingerol and astragaloside IV has a very remarkable promotion effect on the hair growth of androgenic alopecia mice, and the obvious synergistic effect of astragaloside IV on the combination of sanggenon C and 8-gingerol can be seen.

Thus, the composition of the present application can be used for the prevention and treatment of hair loss, in particular for the prevention and treatment of androgenetic alopecia.

Example 2: effect of the composition of the application on in vitro cultured mouse Hair follicle Hair growth and follicular bulb cell proliferation

2.1 in vitro experiments on tentacle hair follicles in mice

1. Mouse tentacle hair follicle organ culture

Killing cervical dislocation of 4-6 week old C57BL/6J mice, cutting off double-sided beard, sterilizing the beard 3 times with 75% alcohol, cutting off the beard pad under aseptic condition, washing 3 times in D-Hanks solution, placing in Williams culture solution, separating hair follicle in long-term under dissecting microscope with microscopic scissors and forceps, and cutting off 1/3 part of hair follicle. Hair follicle culture was performed in 96-well plates, one follicle was placed in each well, and W was added to each wellSerum-free culture medium of illiams culture solution 0.2mL containing transferrin 10mg/L, penicillin 100U/mL, streptomycin 100 μg/mL, etc., and placing into 37 deg.C and 5% CO ₂ Is cultured in a cell culture incubator.

2. Experimental grouping

The experiment is divided into a blank group, a sanggenon C+8-gingerol group, an astragaloside IV group, a sanggenon C+8-gingerol+astragaloside IV group, and 10-15 hair follicles in each group, wherein no medicine is added into the blank group. The final concentration of Morgandone C in Morgandone C+8-gingerol is 5×10 ^-5 g/mL, the final concentration of 8-gingerol is 4×10 ^-5 g/mL. The final concentration of astragaloside IV in astragaloside IV group is 1×10 ^-5 g/mL. The final concentration of Morgandone C in Morgandone C+8-gingerol+astragaloside group is 5×10 ^-5 g/mL, the final concentration of 8-gingerol is 4×10 ^-5 g/mL, astragaloside IV final concentration of 1×10 ^-5 g/mL。

3. Observation of Hair growth

Culturing on 1 st to 7 th days, measuring the length from the hair follicle bottom to the hair top every day by an ocular micrometer under an inverted optical microscope, subtracting the 1 st measurement from the 7 th measurement to obtain the hair growth length, and comparing the hair growth lengths of the groups.

2.2 in vitro experiments on the cells of the follicular bulb of mice

1. In vitro culture of mouse hair follicle bulb cells

Taking back whole skin layer of C57BL/6J milk mouse of 4 days of birth under aseptic condition, adopting two-step enzyme (containing 0.5% separating enzyme and 0.2% type I collagenase) digestion method to separate and obtain hair follicle bulb cell with higher purity, using in 96-well plate of Williams culture solution of blank group of 2.1 part, sanggenon C+8-gingerol group, astragaloside group, sanggenon C+8-gingerol+astragaloside group, each group setting 6 wells, each well volume being 0.2mL, inoculating cell concentration 5×10 ⁴ And each mL. Placing in 37 ℃ and 5% CO ₂ Is cultured in a cell culture incubator.

2. Effect of the composition of the present application on the growth Activity of the cells of the follicular bulb of mice

MTT colorimetric assay was performed 24h and 96h after incubation, respectively, 20. Mu.L of MTT solution (5 mg/mL) was added to each well, incubation was continued for 4h at 37℃and the in-well culture solution was discarded. 0.2mL DMSO was added to each well and the mixture was shaken for 10min to allow the crystals to dissolve well. The absorbance values of each well were measured on a microplate reader at a wavelength of 570nm and the absorbance values of each group were compared.

2.3 statistical methods

The results were analyzed using SPSS 20.0 statistical software and the data are expressed as mean.+ -. SD. The comparison between the groups adopts single-factor analysis of variance, the comparison between the two groups adopts t test, and p <0.05 represents that the difference has statistical significance.

2.4 experimental results

1. Hair growth length

The average hair growth length of each group of hair follicles on day 7 of culture is shown in table 2. The average hair follicle growth length of the test animals of the sanggenon C+8-gingerol+astragaloside IV group was significantly greater than that of the blank group (p < 0.01), and significantly greater than that of the sanggenon C+8-gingerol group (p < 0.05). Morgandone C+8-gingerol group mice also had better whisker hair follicle growth than the blank, but the differences between the experimental results of this group and the blank were not statistically significant. Hair follicle hair growth length between astragaloside IV group and blank group is substantially similar. Astragaloside IV alone was suggested to have substantially no effect in promoting growth of the mouse tentacle hair follicle. From these results, it can be seen that astragaloside IV has a remarkable synergistic effect on the effect of the combination between sanggenon C and 8-gingerol on promoting growth of mouse tentacle hair follicles.

2. Cell growth viability

The results of the mouse hair follicle bulb cell growth viability experiments are shown in Table 2. At 96h post-incubation, the average absorbance values of the sanggenon c+8-gingerol+astragaloside IV group experimental animals were significantly higher than the blank group (p < 0.05) and significantly greater than the sanggenon c+8-gingerol group (p < 0.05). The average absorbance of the sanggenon C+8-gingerol group experimental animals was also higher than that of the blank group, but the difference between the experimental results of the group and the experimental results of the blank group was not statistically significant. The average absorbance between the astragaloside group and the blank group was substantially similar. The astragaloside IV alone has no effect of promoting the growth of the mouse hair follicle bulb cells. From these results, it can be seen that astragaloside IV has a remarkable synergistic effect on the effect of the combination between sanggenon C and 8-gingerol on promoting the growth activity of mouse hair follicle bulb cells.

Table 2: effects of the compositions of the application on growth of mouse tentacle hair follicles and growth viability of mouse hair follicle bulb cells

Note that: * P is p<0.05，**p<0.01, compared with the blank group, ^# ，p<0.05, compared with Morgandone C+8-gingerol group

2.5 conclusion of experiments

The composition provided by the application has the effects of promoting the hair growth of the mouse hair follicle cultured in vitro and promoting the proliferation of hair follicle bulb cells, and the effect is obviously better than that of the combination of sanggenon C and 8-gingerol. Astragaloside IV has little effect on hair growth of mouse hair follicle and cell growth activity of mouse hair follicle bulb compared with blank group. That is, the combination of astragaloside IV with both sanguisorbanone C and 8-gingerol produced a significant synergistic effect in promoting hair growth of hair follicles in mice cultured in vitro.

It will be apparent to those skilled in the art that various modifications and variations can be made to the present application without departing from the spirit or scope of the application. Thus, it is intended that the present application also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. A composition for preventing and treating hair loss by acting on hair follicle tissue, characterized in that: the composition consists of sanggenon C, 8-gingerol and astragaloside IV, wherein the proportion of the sanggenon C, the 8-gingerol and the astragaloside IV is 5:4:1.

2. the composition of claim 1, wherein: the alopecia is one or more of androgenetic alopecia, nervous alopecia, endocrine alopecia, nutritional alopecia, physical alopecia, chemical alopecia, infectious alopecia, congenital alopecia and seasonal alopecia.

3. The composition of claim 2, wherein: the alopecia is androgenetic alopecia.

4. A composition for enhancing the activity of hair follicle tissue in an in vitro culture, comprising: the composition consists of sanggenon C, 8-gingerol and astragaloside IV, wherein the proportion of the sanggenon C, the 8-gingerol and the astragaloside IV is 5:4:1.

5. the composition of claim 4, wherein: the composition improves the microenvironment of hair follicle tissues, promotes the hair follicle tissues in telogen and catagen states to be transformed into long-term hair follicle tissues, further improves the activity of the cultured hair follicle tissues, and remarkably improves the survival rate of transplanted hair follicles.

6. Use of a composition according to any one of claims 1 to 3 or a composition according to claim 4 or claim 5 for the preparation of a product for controlling hair loss by acting on hair follicle tissue.

7. Use according to claim 6, characterized in that: the product is a product for external use, or the product comprises a hair follicle tissue culture solution commonly used in clinic.

8. Use according to claim 7, characterized in that: the product is a medicine or a cosmetic, and the external product is a gel, an ointment, a foam or a spray liquid preparation.