KR20060095135A - Cosmetic composition containing the potentilla fragariodes var. major extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a fern flower extract as an active ingredient. More specifically, the cosmetic composition of the present invention contains a birch flower extract as an active ingredient, can be used safely without side effects on the skin, and also exhibits the activity protecting ability and excellent whitening and moisturizing effect of catalase among the intracellular antioxidant defense factors. .

Sunny * Catalase * Whitening * Moisturizing * Cosmetics

Description

Cosmetic composition containing biscuit flower extract {Cosmetic composition containing the Potentilla fragariodes var. major extracts}

1 is a graph showing the protective effect of the catalase activity of the fern flower extract used in the present invention.

Figure 2 is a graph showing the protective effect of catalase activity of the cosmetic composition containing a birch flower extract of the present invention.

The present invention relates to a cosmetic composition containing a fern flower extract as an active ingredient. More specifically, the cosmetic composition of the present invention contains a birch flower extract as an active ingredient, can be used safely without side effects on the skin, and also exhibits the activity protecting ability and excellent whitening and moisturizing effect of catalase among the intracellular antioxidant defense factors. .

Over time, human skin internally decreases the secretion of various hormones that regulate metabolism, decreases the function and activity of immune cells, and decreases the biosynthesis of immune proteins and bioconstituent proteins necessary for the living body. Externally, due to the destruction of the ozone layer, the amount of ultraviolet rays reaching the surface of the sun's rays increases and the environmental pollution is intensified. And a number of changes occur, such as an increase in blotch.

However, since modern society values young and beautiful appearance, there is a great desire to prevent or solve various skin problems caused by external factors such as internal factors and ultraviolet stress. Accordingly, in order to develop a cosmetic for protecting the skin and improving the skin condition, an active test for solving the above-mentioned purpose is steadily progressing with respect to the vegetable natural product having not only excellent safety but also various beneficial components.

On the other hand, it has the antioxidant effect (Kor. J. Pharmacogn. 29 (2): 79 ~ 85 (1998)) and the anti-wrinkle effect through the active oxygen scavenging effect (JP-A-7-17847). Although known, other uses are not known.

Accordingly, the present inventors searched for natural plants for excellent skin protection and skin condition improvement in a variety of plants, found that the birch extract has excellent catalase activity protection as well as excellent whitening and moisturizing effect. Furthermore, the present inventors have found that the cosmetic composition has excellent catalase activity protection and whitening and moisturizing effect when it contains an extract of yangji flower in an amount of 0.0001 to 20% by weight based on the total weight of the composition, and completed the present invention.

Therefore, it is an object of the present invention to provide a cosmetic composition containing a fern flower extract as an active ingredient.

In order to achieve the above object, the cosmetic composition according to the present invention is characterized in that it contains a yangji flower extract as an active ingredient. In addition, the cosmetic composition of the present invention is characterized in that it contains 0.0001 to 20% by weight, preferably 0.01 to 5% by weight based on the total weight of the biscuit flower extract.

Hereinafter, the cosmetic composition according to the present invention will be described in detail.

Potentilla Fragarioides is Potentilla Fragarioides belongs to the rose family (Rosaceae) (Potentilla fragarioides L. var . Major Maxim.) To refer to as distributed in Korea, Japan, China Oriental in to the shoot as Chi Nature (雉子筵) or increase the digestibility of the stomach It is used to treat acidosis, and it is known that root is used as hemostatic agent as natural root. (+)-Catechin, quercitrin, caffeic acid, isoquercitrin, 4- 0 -caffeoyl L-throleic acid (threonic acid), quercetin- 3-0 -beta-D-glucopyranosyl-beta-D-xylopyranosi de have been reported to be isolated In addition, the case of (+)-catechin (catechin) is known to have the effect of lowering the permeability and fragility of the capillaries and promote blood circulation as well as antioxidant activity.

Potentilla genus plants that grow in Korea besides yangji flower include Potentilla chinensis Ser., P. cryptotaeniae Masim., P. dickinsii Fr. et Sav., P. discolor Bunge ), P.freyniana Bornm., P. kleiniana Wight et Arnott, P. matsumurae , P. paradoxa Nutt., P. yokusaiana Makino The cosmetic composition according to the present invention preferably contains a hydrangea among the Potentilla genus plants as an active ingredient.

Yangji flower extract used in the present invention can be prepared by a known extraction method as follows. For example, not only the leaching liquid obtained by leaching and transferring it from a fern, but also the concentrate obtained by partially or fully concentrating the leaching liquid, or a stagnation, premises, periodical, and flow extract prepared by drying the above concentrate again are used.

<Method of producing a sun flower extract>

Sunny flowers are dried by any method such as natural drying or forced drying, and finely chopped, followed by polar solvents such as water, ethanol, butanol and acetone; Nonpolar solvents such as ether, hexane, benzene, chloroform and ethyl acetate; A mixed solvent of the nonpolar solvent and the polar solvent; Using a solvent such as alkaline water, or vegetable oils such as soybean oil and sesame oil, the leaching treatment is carried out by any method such as cold sedimentation, percolation, and warm sedimentation to obtain a leaching substance containing the active ingredient. The leaching treatment is about 12 to 96 hours for cold needle and percolation, and depending on the type and temperature of solvent used for warm needle, but suitably for about 0.5 to 24 hours at a temperature close to the reflux temperature of the solvent. In particular, it is preferable to use a tincture, a liquid extract or an extract extracted from the hydrous alcohol.

On the other hand, when using the yangji extract according to the present invention in the cosmetic composition, it is not particularly limited, but contains 0.0001 to 20% by weight, preferably 0.01 to 5% by weight relative to the total weight of the composition. This is because when the amount is less than 0.0001% by weight, the effect is insignificant, and when it exceeds 20% by weight, problems may occur in skin side effects or formulation stability.

In addition, the cosmetic composition of the present invention, in addition to yangji flower extract, other components that can give a synergistic effect to the skin protective effect, preferably within the range that does not impair the catalase activity protection and whitening and moisturizing effect for the purpose of the present invention It may contain.

The cosmetic composition of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water It can be formulated into cosmetics such as packs, powders, body lotions, body creams, body oils and body essences.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

Preparation Example Preparation of Solar Flower Extract

The sun flower was sliced and washed with purified water, and 100 g of dried dry matter was put in 1 L of 70% ethanol, and extracted by cooling at room temperature for 48 hours. Next, the extract was filtered with 0.45 μm filter paper and dried at 50 ° C. using a rotary evaporator to obtain a dry weight of 10 g.

Experimental Example 1 Measurement of Catalase Protective Effect on Human Skin Cell Lines

The cell line used to measure the catalase protective effect of hydrangea extracts was developed by Dr. Human keratinocytes HaCaT cell line (Human keratinocytes HaCaT cell line) received from Fusenig.

First, the cell line was divided into 6.0 × 10 ⁶ cells in a 150 ㅧ 150 culture plate and incubated for 1 day, and then the cells were cultured with DMEM (Dulbecos modified eagles medium) containing 10% by volume of fetal bovine serum (FBS). , GIBCO BRL, Life Technologues Co., Ltd.) was dispensed in each of the treated medium at a concentration of 0.005% by volume of the extract of Yangji flower of the above preparation and incubated for 1 day at 37 ℃, 5% CO ₂ conditions. 1X phosphate buffer (PBS, phosphate buffered saline, Sigma, St. Louis, MO, USA) was used for washing after incubation. Next, using ultraviolet B lamp (Model: F15T8, UVB 15W, Sankyo Dennki, Japan) treated with ultraviolet light at an intensity of 30mJ / ㎠, incubated for 1 day at 37 ℃, 5% CO ₂ conditions Harvest was used to measure catalase activity. As a positive control for comparing the results, the concentration of vitamin C was 10 μM. In addition, the catalase activity is Bud huin's method [Biochem. J. (1964) 92: 179-184], the catalase activity was expressed as a percentage of the activity of the control group that did not irradiate with UVB in 100%, and calculated by Equation 1 below. It was.

(A: activity of control group, B: activity of UVB treated group and each experimental group)

In addition, the result of measuring the catalase protection effect of the experimental group treated with the Yangji flower extract compared to the group irradiated with UVB was expressed in%, it was calculated by the following equation (2). The results are shown in Table 1 and FIG. 1.

(A: catalase activity of control group, B: catalase activity of UVB treated group, C: catalase activity of each experimental group)

sample Catalase Protective Activity (%) Control 100 UVB treatment group 73.2 Vitamin C (final concentration 10 μM) 86.7 Preparation example (final concentration 0.005% by volume) 90.4

As can be seen in Table 1 and Figure 1 below, it was confirmed that the fern flower extract has the effect of protecting the catalase activity reduced by ultraviolet light, such as vitamin C.

[Examples 1 and 2 and Comparative Examples 1 and 2]

To prepare a cream containing the Yangji flower extract (Examples 1 to 2) in the composition of Table 2, a cream containing vitamin C (Comparative Example 2) and cream containing neither of Yangji extract or vitamin C (Comparative Example) 1) was prepared.

ingredient Content (% by weight) Example 1 Example 2 Comparative Example 1 Comparative Example 2 glycerin 3.0 3.0 3.0 3.0 Butylene glycol 2.5 2.5 2.5 2.5 Propylene glycol 2.0 2.0 2.0 2.0 Squalene 5.0 5.0 5.0 5.0 Capric / Capric Triglycerides 3.0 3.0 3.0 3.0 Stearic acid 0.5 0.5 0.5 0.5 Carboxy Vinyl Polymer 0.3 0.3 0.3 0.3 Liquid paraffin 7.0 7.0 7.0 7.0 Beeswax 4.5 4.5 4.5 4.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 Polysorbate 60 1.5 1.5 1.5 1.5 Glycerin Monostearate 4.5 4.5 4.5 4.5 Sorbitan monosulfate 0.05 0.05 0.05 0.05 antiseptic Quantity Quantity Quantity Quantity Vitamin C - - - 5.0 Yangji flower extract (production example) 1.0 0.1 - - Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Example 3 and Comparative Example 3

Example 3 and Comparative Example 3 were prepared in the formulation of the lotion with the composition of Table 3 below.

Compounding ingredients (content; wt%) Example 3 Comparative Example 3 Purified water Remaining amount Remaining amount Yangji flower extract (production example) 0.1 - Vegetable Cured Oil 1.50 1.50 Stearic acid 0.60 0.60 Glycerol Stearate 1.00 1.00 Stearyl alcohol 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 Caprylic / Capric Triglycerides 11.00 11.00 Cyclomedicon 6.00 6.00 Preservative, incense Quantity Quantity Triethanol amine 0.1 0.1

Experimental Example 2 Measurement of Catalase Protective Effect on the Skin of Hairless Mouse

Using a 10-week-old hairless mouse, experiments were carried out on the protective effect of the extract of fern against catalase activity of the skin irradiated with ultraviolet (UVB).

The creams of Examples 1 and 2 and Comparative Examples 1 and 2 were applied to the back of the hairless mice once a day for 4 days. The amount of ultraviolet light B irradiated during the test period was 400mJ / cm 2 using Waldmann UV800 (Herbert Waldmann GmbH & E Philips TL / 12 lamp emitting 280 ~ 305nm). Biopsy of the dorsal skin of hairless mice was used for analysis, and the analysis items were the same as described in Test Example 1 above. In addition, the results are calculated by the equations (1) and (2) described in Test Example 1 shown in Table 4 and FIG.

sample Catalase Protective Activity (%) Control 100 Example 1 96.7 Example 2 90.4 Comparative Example 1 80.0 Comparative Example 2 91.5 NOTE The data above is an average of four measurements.

As can be seen in Table 4 and Figure 2, it was found that Examples 1 and 2 containing the yangji flower extract of the present invention protects catalase activity reduced by ultraviolet light, such as vitamin C.

Test Example 3 Evaluation of Melanin Inhibitory Activity Using B16 / F10 melanoma Cells

Using the yangji flower extract prepared in the above preparation as a test substance to the culture solution of B16 / F10 melanoma cells (Korea Cell Line Bank) at a constant concentration and incubated for 3 days, the culture solution was removed, washed with PBS and dissolved in 1N NaOH Absorbance was measured at 405 nm. As a control, the cells without test substance were compared with the melanin content in the control group, and the whitening effect was measured by measuring the inhibition of melanin production of the test substance. To calculate the melanin production inhibition rate according to the following equation 3 is shown in Table 5 the results.

Melanin production inhibitory effect sample Melanogenesis inhibition rate (%) Control group (no addition) - Preparation example (final concentration 0.001% by weight) 32.8 Preparation example (final concentration 0.005% by weight) 41.5 Hydroquinone (final concentration 0.0001% by weight) 44

As can be seen from Table 5, there was no significant difference even when compared to the existing hydroquinone which is a whitening component, it was found that the birch flower extract has an excellent whitening effect.

Test Example 4 Measurement of Glycosaminoglycan Production in Human Skin Fibroblasts

In order to measure the moisturizing effect of the birch flower extract of the present invention, glycosaminoglycans (GAG) which fills the rest of the skin other than collagen and elastin, supports the skin and holds moisture in the dermis. Quantification First, human fibroblasts were cultured by placing 5 × 10 ⁴ cells in a 24-well cell culture plate using phenol red-free medium and 5% fetal calf serum treated with charcoal. After 18 hours, the test material was treated to the concentration shown in Table 6 below, and again after 18 hours, the amount of GAG secreted in the supernatant was measured. For analysis, Blyscan Glycosaminoglycan assay kit manufactured by Biocolor, UK was used. The principle of GAG measurement is the binding of sulfated GAG and 1,9-dimethyl methylene blue with positive charges to proteoglycans to form a precipitate, which is dissolved and quantified. The results in Table 6 below are shown as relative ratios to the control group 100.

Test substance Concentration (ppm) Glycosaminoglycan Biosynthesis (%) Yangji Flower Extract (Manufacturing Example) 10 140 One 131 0.1 120 Control 100

As can be seen in Table 6, it was found that the glycosaminoglycans were synthesized more than the control group when treated with birch flower extract.

Test Example 5 Measurement of Increased Skin Moisturizing Power in Human Body

The effect of improving the skin moisturizing power of Example 3 and Comparative Example 3 was measured. Thirty adult men and women in their fifties and sixties with skin dryness were divided into three groups, and the lotion of Example 3 and Comparative Example 3 was applied to the face twice daily for four weeks in each group. Coniometer at constant temperature and humidity conditions (24 ° C, 40% relative humidity) before start of application, after 1 week, 2 weeks, 4 weeks after application, and after 2 weeks (total 6 weeks) after application was stopped. Skin moisture content was measured by. The results are shown in Table 7 below, and the results in Table 7 indicate the percentage increase of the measured value after treatment for a period of time based on the Koniometer value measured immediately before the start of the test. The Koniometer is a skin moisture meter that measures the amount of moisture present on the skin by measuring the conductivity of the epidermis.

Separately, at the end of the test, the test subjects were asked to fill out a questionnaire and subjected to mechanical evaluation and subjective efficacy evaluation, and the results are shown in Table 8 below.

Applicator Moisture Growth Rate (%) 1 week past 2 weeks past 4 weeks past After 6 weeks Example 3 43 50 58 48 Comparative Example 3 33 37 35 12

As shown in Table 7, in the group coated with the material of Example 3 containing the birch flower extract, the moisture content of the skin was further increased compared to the group coated with Comparative Example 3, and 2 weeks after the application of the test substance was stopped. Later, after 6 weeks after the measurement of skin moisture, the value was similar to that after 1 ~ 2 weeks, and it was found that the skin moisture was maintained by the birch flower extract to some extent even without applying the test substance.

Applicator Respondents (Personal) Very good Good usually Inadequate Example 3 2 5 3 0 Comparative Example 3 One 3 4 2

As shown in Table 8, it was confirmed through the questionnaire about the improvement effect of dry skin, when the Example 3 containing the birch flower extract was applied, the skin dryness was improved.

Test Example 6 Safety and Human Patch Testing

In order to determine the degree of skin irritation of the cosmetic according to the present invention, a skin patch test on the cosmetic composition according to Example 3 was conducted to 15 subjects. 20 μl of Example 3 was added to a fin chamber and patched to the upper arm of the human body for 24 hours. The skin reaction was examined within 1 hour after the patch was removed, and then examined again the following day (after 48 hours). The skin reaction was judged by the inspection criteria of Table 9, and the average response rate was calculated according to the following formula. The results are shown in Table 10 below.

Inspection standard of human patch test Skin reaction Sign and score Negative susceptibility without negative suspicious erythema vesicles, moderate reaction with erythema, papule vesicles, erythema, papules, strong vesicles, alveolar reaction -: 0 +/-: 1 +: 2 ++: 3 +++: 4

Average Response Rate (%) = [∑ ( _{24 hours after} respondents × score) + ∑ ( _{48 hours after} responders) × 100 / (Total number of tests × maximum score × total number of subjects)

Test substance Number of subjects responding after 24 hours (persons) Number of subjects responding after 48 hours (persons) Average response rate (%) - +/- + ++ +++ - +/- + ++ +++ Example 3 15 0 0 0 0 15 0 0 0 0 0 ＊ judgment criteria for average response rate (%) of human patch test; Less than 1: No irritation, Less than 1: 3: Light stimulation, Less than 3 ~ 5: Medium stimulation, More than 5: Strong stimulation

As can be seen from Table 10, the cosmetic composition according to the present invention can be said to be a safe substance to the human body because it belongs to the non-irritating range.

On the other hand, the formulation of the cosmetic composition containing the yangyang extract of the present invention can be selected arbitrarily, it can be prepared in various forms, such as nutrition cream, nutrition lotion, massage cream, nutrition essence, supple cosmetics, which are conventional cosmetic formulations.

Formulation Example 1 Nutritious Longevity (Milk Lotion)

Nutritious longevity containing a birch flower extract was prepared according to a conventional method as follows.

Ingredient Content (% by weight) Sun flower extract 1.0 Squalane 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 2 Softening Cosmetic (Skin Lotion)

A softening lotion containing a birch flower extract was prepared according to a conventional method as follows.

Ingredient Content (% by weight) Sun flower extract 1.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 3 Nutrition Cream

A nutritious cream containing a birch flower extract was prepared according to a conventional method as follows.

Ingredient Content (% by weight) Sun flower extract 1.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.5 PEG60 Cured Castor Oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Caprol Triglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic Quantity Pigment Quantity Spices Quantity Purified water Remaining amount

Formulation Example 4 Massage Cream

Massage cream containing a birch flower extract was prepared according to a conventional method as follows.

Ingredient Content (% by weight) Sun flower extract 0.5 Beeswax 10.0 Polysorbate 60 1.5 PEG 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Formulation Example 5] Pack

A pack containing a birch flower extract was prepared according to a conventional method as follows.

Ingredient Content (% by weight) Sun flower extract 0.5 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 6 Gel

A gel containing a birch flower extract was prepared according to a conventional method as follows.

Ingredient Content (% by weight) Sun flower extract 0.5 Ethylenediamine Sodium Acetate 0.05 glycerin 5.0 Carboxy Vinyl Polymer 0.3 ethanol 5.0 PEG 60 Cured Castor Oil 0.5 Triethanolamine 0.3 Preservative, coloring, flavoring Quantity Purified water Remaining amount

As described above, the cosmetic composition comprising the yangyang flower extract according to the present invention as an active ingredient can be used in cosmetics having a safety against human skin and a catalase activity protection effect as well as a whitening and moisturizing effect.

Claims (4)

A catalase active protective cosmetic composition, characterized in that it contains an extract of fern flowers as an active ingredient. A cosmetic composition for skin whitening, which comprises a hydrangea extract as an active ingredient. A cosmetic composition for moisturizing skin, which contains a hydrangea extract as an active ingredient. The composition according to any one of claims 1 to 4, wherein the birch extract is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition.

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