KR20060008020A - Processed ginseng - Google Patents

Abstract

The present invention relates to processed ginseng or extracts thereof by treating ginseng with acidic electrolyzed or alkaline electrolyzed water to increase the content of ginsenosides Rg2, Rg3 and Rg5, and the present invention is very environmentally friendly and has a high content of specific ginsenosides. You can get processed ginseng with increased.

Ginsenoside, acidic electrolyzed water, alkaline electrolyzed water, processed ginseng

Description

Processed Ginseng

1 is a schematic diagram showing a conventional electrolytic water generation principle.

The present invention relates to processed ginseng or extracts thereof by treating ginseng with acidic electrolyzed or alkaline electrolyzed water to increase the content of ginsenosides Rg2, Rg3 and Rg5.

Various ginsenosides present in ginseng are known to have excellent pharmacological activity in the circulatory and central nervous system, antioxidant, anticancer, antidiabetic and immune function control. Glycosides such as ginsenosides selectively bind one or several sugars to aglycone, which is a basic form, and its pharmacological action is different depending on the type and type of sugar bond.

Representative ginsenosides known from ginseng are shown in Table 1 and, like the known efficacy of ginseng, the range of efficacy is also very broad. In ginseng or red ginseng, ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 are present in large amounts, but ginsenosides Rg2, Rg3, Rg5 are present in trace amounts. Jina Na, a product of Pharmaton Co., Ltd., presents only seven major ginsenosides as indicator components, and it is difficult to expect the effects of trace ginsenosides.

Table 1. Pharmacological action of 10 reported ginsenosides

Ginsenoside Drug operation Rb1 Central inhibition and mental stability, aggressive behavior inhibition, analgesic, anticonvulsant, high cholesterol lowering, anti-anxiety, cholesterol biosynthesis promotion, bone marrow cell DNA, RNA, protein and lipid synthesis promotion, protein synthesis promotion , Promotes neuronal survival, Acetylcholine release, Memory improves, Platelet aggregation, Lipid peroxidation, Vasodilation, Cholesterol metabolism, Anti-inflammatory, Liver injury protection Rb2 Promotes sugar and fat metabolism, antidiabetic, inhibits lipolysis, hypercholesterol lowering and anti-arteriosclerosis, promotes corticosteroid secretion, promotes protein lipid synthesis, promotes liver cell proliferation and DNA synthesis, immune control, analgesic Action, promotes cholesterol metabolism Rc Analgesic, corticosterone secretion promoting, liver serum cholesterol, RNA, synthesizing, bone marrow cell DNA, RNA, protein, lipid synthesis promoting, renal glomeruli inhibition Rd Corticosteroids, corticosteroid secretion, renal glomeruli inhibition Re Adrenal cortex stimulating hormone, corticosteroid secretion, bone marrow cell DNA, RNA, protein, lipid synthesis promoting, analgesic, smooth muscle cell proliferation inhibition, vasodilation, cholesterol metabolism promoting action, liver injury protection, antihypertensive stress Action Rf Pain Inhibition, Lipid Peroxidation Inhibition, Alcohol Induced Brain Disorder Defense Rg1 Immune function enhancement, platelet aggregation inhibition, antithrombin, memory and learning function enhancement, protein synthesis promotion, antifatigue, antistress, central excitability, vasodilation, high temperature environment and endogenous pyrogens Action, improving stressful behavioral disorders, anti-inflammatory and renal blood flow enhancement, neuronal cell survival rate, cholesterol metabolism, anti-inflammatory, liver cell proliferation and DNA synthesis, liver damage protection, adrenal cortex stimulation Hormone secretagogue Rg2 Platelet aggregation inhibition, acetylcholine-induced catecholamine secretion and intracellular calcium influx, antithrombin, glandular activation, memory loss, smooth muscle cell proliferation Rg3 Cancer cell metastasis inhibitory, platelet aggregation and antithrombotic action, liver injury inhibitory action, vascular relaxation action, anticancer drug resistance action, brain neuroprotection Rg5 Cancer cell metastasis inhibitory, platelet aggregation and antithrombotic action, liver injury inhibitory action, vascular relaxation action, anticancer drug resistance action, brain neuroprotection

Recently, as interest in trace ginsenosides contained in ginseng has been reported and such trace ginsenosides have particularly excellent anticancer activity, studies to increase the content of specific trace ginsenosides more efficiently are being conducted. have.

Looking at the existing process for increasing trace ginsenoside content,                         

(1) enzyme treatment method (Republic of Korea No. 329259)

Ginseng irradiated with enzymes isolated from microorganisms to the irradiated saponin fraction. Because of the enzyme treatment, there is an uneconomical disadvantage of removing the reaction solution and enzyme.

(2) A method of high temperature treatment (Korea No. 329259), which relates to processed ginseng, for example, by increasing the content of trace ginsenosides Rg3 and Rg5 by heating the ginseng at a high temperature. The ginsenoside Rg3 and Rg5 content is increased by heating at a high temperature of ˜180 ° C. for 0.5 to 20 hours. The disadvantage is that it is uneconomical at high temperature and high pressure.

(3) acid treatment method (Arzneimittel-Forschung Drug Research, 30, 936-943, 1980)

The method of increasing the trace ginsenoside Rg3 and Rg5 content by treating the acid while heating the extract of ginseng is known, but there is a disadvantage that it is not easy to remove the acid used is not environmentally friendly.

Accordingly, the present inventors have repeatedly conducted extensive studies to increase the content of certain trace ginsenosides in a more environmentally friendly and good yield, and surprisingly, when treating acidic electrolytic or alkaline electrolyzed water to ginseng, trace ginsenosides Rg2, Rg3 and The present invention was completed by confirming that the content of Rg5 can be increased.

Accordingly, it is an object of the present invention to provide a processed ginseng or an extract thereof, which is obtained by treating acid electrolytic water or alkaline electrolytic water with ginseng.                         

Another object of the present invention is to provide a processed ginseng or an extract thereof including red ginseng or white ginseng powder, an extract thereof, or a lyophilized product thereof in addition to the processed ginseng or an extract thereof.

In order to achieve the above object, the present invention provides a processed ginseng, characterized in that obtained by treating ginseng with acidic electrolytic water or alkaline electrolytic water.

In the present invention, the ginseng is preferably treated with acidic electrolytic water or alkaline electrolyzed water at a temperature of 50 to 80 ° C, and further processed ginseng is processed by increasing ginseng for 0.5 to 15 hours at a temperature of 90 ° C to 120 ° C. You can get ginseng.

As described above, when adding acid electrolytic water or alkaline electrolyzed water and increasing the temperature, the temperature at the time of steaming is not limited so long as it is a temperature that can effectively increase the content of Rg2, Rg3 and Rg5 of ginsenosides. Processed ginseng may be prepared by increasing the ginseng for 0.5 to 15 hours, preferably 0.5 to 10 hours, preferably at a temperature of 90 ° C to 120 ° C, particularly preferably at a temperature of about 90 to 110 ° C.

In addition, the treatment amount of the acidic electrolytic water or alkaline electrolyzed water may be used in a weight ratio of 5 to 100 times, more preferably 20 to 50 times with respect to the raw ginseng.

Acid and alkali electrolytic water used in the present invention can be prepared using a device capable of producing a conventional electrolytic water, the production principle and production method of electrolytic water is as follows.

Mineral ions contained in purified water are moved by electric stimulation, and the (-) electrode contains hydroxyl ions (OH ⁺ ), sodium ions (Na ⁺ ), calcium (Ca ²⁺ ), magnesium (Mg ²⁺ ) and potassium Alkali electrolytic water is generated by (K ⁺ ), and acid electrolytic water composed of anions such as hydrogen ions (H ⁺ ), chlorine ions (Cl ⁻ ) and sulfuric acid (SO ₄ ^2- ) is generated at the (+) electrode.

The composition and environmentally friendly properties of acidic and alkaline electrolyzed water are as follows:

-Characteristics of acidic electrolyzed water

(1) Acidic electrolyzed water is a strong / weakly acidic substance with a pH of 2.5-4.0, and it moisturizes the skin with an astrogent.

(2) Can be used to rinse your mouth to keep your mouth clean.

(3) It is used for washing food and dishes.

-Characteristics of Alkaline Electrolyzed Water

(1) Alkaline ionized water is pH 8.5-10, which plays a good role in keeping human body fluids at pH 7.3-7.4.

(2) The mass of water molecules (clusters) is half the size of ordinary water, so it is quick and easy to absorb in the body, thereby promoting metabolism in cells.

(3) Alkaline ionized water contains a lot of minerals (calcium, magnesium, potassium, sodium, etc.) that are essential to our body.

-Eco-friendly Characteristics of Acid / Alkali Electrolyzed Water

The acid / alkali electrolyzed water used to induce acid hydrolysis is produced through the electrolysis process after firstly purifying the tap water which is generally used. There is no irritating odor and other hazards that can be dangerous to the operator even during process use.

As used herein, the term "processed ginseng" refers to ginseng that has been processed and improved in function than the original, and includes ginseng or powder thereof processed according to the present invention.

In the processed ginseng of the present invention. The content of ginsenosides Rg2, Rg3 and Rg5 is greatly increased compared to the content contained in the original, for example, may be increased 5 to 50 times compared to the original.

Therefore, when using acidic and / or alkaline electrolyzed water to increase the content of trace ginsenosides as described above, the operation can be performed very environmentally and efficiently. You can cut it off completely.

The present invention also provides an extract of the processed ginseng or a C _1-4 alcohol such as methanol, ethanol, propanol, butanol, or a mixed solvent thereof or a lyophilized extract of the extract.

The processed ginseng extract of the present invention may be extracted after processing the ginseng as described above, water or C _1-4 alcohol, or a mixed solvent extract of ginseng, and then prepared ginseng extract acidic electrolytic water Alternatively, the processed ginseng extract of the present invention may be obtained by treating with alkaline electrolytic water.

In one preferred method, the water or C _1-4 alcohol or mixed solvent extract of ginseng is treated with acid electrolytic or alkaline electrolyzed water in a weight ratio of 5 to 100 times by weight at a temperature of 50 to 100 ° C., and dried under reduced pressure to obtain the invention. Processed ginseng extract can be obtained.

The processed ginseng of the present invention or extract thereof may further include red ginseng or white ginseng powder, extracts thereof, or lyophilized products thereof. Preferably, red ginseng or white ginseng powder, extracts thereof, or lyophilized products thereof may be included in a ratio of 0.01 to 100 based on the weight of the processed ginseng of the present invention.

As described above, by mixing red ginseng or white ginseng powder, its extract, or its lyophilized product with the processed ginseng of the present invention having an increased content of Rg2, Rg3 and Rg5, other ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf And by increasing the content of Rg1 can provide a standard ginseng product containing all 10 major saponins of ginseng. In the standard ginseng product, i) the content of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf and Rg1, and ii) the content ratio of ginsenosides Rg2, Rg3 and Rg5 is preferably 0.1 to 10: 0.1-10, more preferably 1: 0.1-10, particularly preferably 1: 0.1-5.

The red ginseng or white ginseng extract may be extracted with water or a conventional organic solvent, such as C _1-4 alcohol, or a mixed solvent thereof.

In the present invention, the ginseng is Panax ginseng , Panax japonicum ( P. japonicum ), Panax quinquefolium ( P. quinquefolium ), Panax ginseng ( P. notoginseng ), Trifolium ginseng ( P. trifolium) ) And P. pseudoginsen g ( P. pseudoginsen g) can be selected and used without limitation, and the roots or stems, leaves, flowers, ground parts such as flowers, or an outpost can be used.

The processed ginseng of the present invention may be formulated by a method known in the pharmaceutical field, and may be mixed with the extract itself or a pharmaceutically acceptable carrier, excipient, etc., to prepare a conventional pharmaceutical preparation, for example, a liquid such as a drink, a syrup, It may be formulated in a capsule or the like, they may be administered orally or parenterally. The processed ginseng of the present invention is preferably administered orally before and / or after meals as a drink for quick effect.

The liquid, capsules and the like containing the processed ginseng of the present invention may be used as a medicine or health functional food, and the term "health functional food" used in the present invention refers to a raw material or ingredient having useful functionality to the human body. Refers to food products manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills.

The processed ginseng of the present invention is appropriately selected according to the absorbency, excretion rate of the active ingredient in the body, age and weight of the patient, sex and condition, the severity of the disease to be treated, etc. 0.5-10 ml, which is taken 1 to 3 times a day. In other formulations, an appropriate amount calculated in consideration of the liquid dosage can be administered orally.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited by them in any way.

Example

A. Ginseng Extract  acid Electrolytic water  And alkali Electrolytic water  Process

Acid electrolyzed water (pH 2.4) and alkaline electrolyzed water (pH 12.4) were used as solvents for the production of ginseng extract with increased trace ginsenoside content (Rg2, Rg3 and Rg5). The electrolyzed water was manufactured using an electrolytic water maker of Isu-Tech.

In order to proceed with the hydrolysis of ginseng extract, 100 g of ginseng ethanol extract was quantified and placed in a 5L round flask, and 2L of acidic electrolyzed water (pH 2.4) and alkaline electrolyzed water (pH 12.4) were respectively added. The reaction was advanced by applying heat to a 5L round flask containing acidic electrolytic or alkaline electrolyzed water at 60 ° C. for 2 hours. After 2 hours, the mixture was concentrated under reduced pressure until the viscosity of the concentrate was 24 Brix, and then lyophilized.

B. Ginseng Ground Extract  acid Electrolytic water  And alkali Electrolytic water  Process

Acid electrolyzed water (pH 2.4) and alkaline electrolyzed water (pH 12.4) were used as solvents for preparing the ginseng leaf extract with increased trace ginsenoside content (Rg2, Rg3 and Rg5). The electrolyzed water was manufactured using an electrolytic water maker of Isu-Tech.

In order to proceed with the hydrolysis reaction of the ginseng leaf extract 100 g of ginseng leaf ethanol extract was weighed into a 5L round flask, and 2L of acidic electrolyzed water (pH2.4) and alkaline electrolyzed water (pH 12.4) were added, respectively. The reaction was advanced by applying heat to a 5L round flask containing acidic electrolytic or alkaline electrolyzed water at 60 ° C. for 2 hours. After 2 hours, the mixture was concentrated under reduced pressure until the viscosity of the concentrate was 24 Brix, and then lyophilized.                     

C. Preparation of Processed Ginseng

1. Process of acid electrolytic and alkaline electrolyzed water of raw ginseng and ground of ginseng

The red ginseng machine (Sogang ENG Co., Korea), concentrator (EYELA, Japan), and freeze dryer (Ilshin Lab Co., Ltd., Korea) used for the manufacture of processed ginseng used the equipment owned by Unizen Material Development Team. Ginseng 4 years root (Geumsan) and ginseng ground part (Gumsan) were used. Acid electrolytic water (pH 2.4) and alkaline electrolytic water (pH 12.4) were manufactured using the electrolytic water maker of Isu-Tech.

In order to calculate the optimum concentration and treatment method of acidic electrolytic water and alkaline electrolytic water, 100 g of 4 years old ginseng and 100 g of ground ginseng were quantified and placed in each plastic container, and 1.5L of acidic electrolytic water and alkaline electrolytic water were used as treatment solvents, respectively. . One plastic container of each of the plastic containers into which the acidic electrolytic water and the alkaline electrolyzed water was added was heated at 70 ° C. for 3 hours in a water bath to apply heat. It was left at room temperature for 4, 6, 8, 10, 24, 48 hours.

2. Preparation of Red Ginseng and Extract

Acidic electrolyzed water and alkaline electrolyzed water were removed from the ginseng obtained by treatment as described in item 1 above, and the red ginseng temperature of the raw material treated with acidic electrolyzed water and alkaline electrolyzed water in order to calculate the optimum jeungsam temperature and time for further hydrolysis of sugars. And the time was given the conditions of 120 ℃ (8hr), 100 ℃ (3hr), 80 ℃ (8hr), 80 ℃ (3hr), respectively, proceeded to the jeungsam process. Processed ginseng was extracted with 70% ethanol at 80 ° C. for 6 hours and 45 ° C. for about 5 hours.

The extract was diluted with high viscosity (about 65-70Brix) with warm water to reduce the pumping effect due to temperature rise and decompression during lyophilization, and the viscosity was reduced to about 24Brix and frozen for about 2 days at -70 ° C. After completing the freeze, the reaction was lyophilized for 2 days at -70 ° C and 10 mtorr.

Content analysis

The HPLC used for analysis of the reactants used a HITACHI (pump; L-7100, detector; L-7455, interface; D-7000, column oven; L-7300, automated sampler; L-7200) system, The analysis conditions were Capcell PAK C18 (5㎛), 3.0 * 75mm as the stationary phase, and the mobile phase conditions were A solvent: acetonitrile, B solvent: water and gradient conditions. The flow rate was 0.5mL / min and the total analysis time was 110 minutes. The temperature of the column oven was set to 40 ° C., the injection amount of the sample was 10 μl, and detected at 203 nm with a UV detector. Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 were separated within 60 minutes, and Rg2, Rg3, Rg5 were separated after 70 minutes. Samples for analysis of the processed ginseng lyophilisate prepared according to the method of the present invention were prepared in methanol at a concentration of 2 mg / ml. Ginsenoside standard samples were prepared at a concentration of 0.2 mg / ml. The analysis results are shown in Table 2 below.

TABLE 2

Sample Name (20R) -Rg2 (mg / g) 20 (S) -Rg2 (mg / g) 20 (R) -Rg3 (mg / g) 20 (S) -Rg3 (mg / g) Rg5 (mg / g) One Ginseng Extract 0.04 0.08 0.04 0.08 0.11 2 Acid Electrolyzed Water Product (Step A) 0.67 1.2 0.42 0.52 1.2 3 Alkaline Electrolyzed Water Product (Step A) 0.51                                          0.8                                          0.21                                          0.44                                          0.81                                          4 Ginseng ground extract 0.03 0.02 0.09 0.07 0.10 5 Acidic Electrolyzed Water Product (Step B) 0.42 0.56 0.34 0.59 0.95 6 Alkaline Electrolyzed Water Product (Step B) 0.32 0.12 0.24 0.43 0.75 7 Ginseng 0.02 0.03 - - - 8 Acid electrolytic water 120 ℃, 8hr treatment (C stage) 0.21 0.47 0.12 0.13 0.84 9 Acid electrolytic water 100 ℃, 3hr treatment (C stage) 0.36 0.42 0.22 0.24 0.86 10 Acidic electrolytic water 80 ℃, 8hr treatment (C stage) 0.12 0.03 - - 11 Acidic electrolytic water 80 ℃, 3hr treatment (C stage) 0.09 0.04 - - - 12 Alkaline electrolytic water 120 ℃, 8hr treatment (C stage) 0.13 0.32 0.09 0.11 0.48 13 Alkaline electrolytic water 100 ℃, 3hr treatment (C stage) 0.32 0.39 0.18 0.27 0.61 14 Ginseng Branch 0.01 0.03 - - - 15 Acid electrolytic water 120 ℃, 8hr treatment (C stage) 0.17 0.36 0.15 0.11 0.75 16 Acid electrolytic water 100 ℃, 3hr treatment (C stage) 0.32 0.38 0.25 0.33 0.89 17 Acidic electrolytic water 80 ℃, 8hr treatment (C stage) 0.19 0.09 - - 18 Acidic electrolytic water 80 ℃, 3hr treatment (C stage) 0.08 0.07 - - - 19 Alkaline electrolytic water 120 ℃, 8hr treatment (C stage) 0.17 0.29 0.07 0.08 0.44 20 Alkaline electrolytic water 100 ℃, 3hr treatment (C stage) 0.28 0.34 0.15 0.19 0.46

As shown in Table 2, as a result of treating the ginseng extract (step A) and the ginseng ground portion extract (step B) and the ginseng raw material and the ginseng ground portion (step C) with acidic electrolytic water and alkaline electrolyzed water, the content of trace ginsenosides is weaker than that of the raw materials. The results showed a 10-fold increase.                     

Formulation Example 1 Preparation of Liquid

Sample 6 3g

10 g of sugar

10 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components were mixed and sterilized according to a conventional method for preparing a liquid to prepare a liquid.

Formulation Example 2: Preparation of Liquid

Sample 2 3g

10 g of sugar

10 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components were mixed and sterilized according to a conventional method for preparing a liquid to prepare a liquid.

Formulation Example 3 Preparation of Capsule

Sample 9 500 mg

Lactose 50mg

Starch 50mg

Talc 2mg

Magnesium stearate appropriate amount

The capsules were prepared by mixing the above ingredients and filling gelatin capsules according to a conventional method for preparing capsules.

Formulation Example 4 Preparation of Capsule

Sample 16 500 mg

Lactose 50mg

Starch 50mg

Talc 2mg

Magnesium stearate appropriate amount

The capsules were prepared by mixing the above ingredients and filling gelatin capsules according to a conventional method for preparing capsules.

Formulation Example 5 Preparation of Beverage

A mixture was prepared by adding 6 wt% of sample 13 and 5 wt% of fructose, 0.1 wt% of citric acid, and an appropriate amount of lemon flavor, and then purified water was added to prepare a beverage.

As can be seen from the above, the present invention is very eco-friendly and can produce a processed ginseng with increased content of ginsenosides Rg2, Rg3 and Rg5 with high efficiency, ginsenoside Rg2, without any environmental hazards Processed ginseng having inherent pharmacological effects of Rg3 and Rg5 can be provided.

Claims (9)

Processed ginseng obtained by treating ginseng with acidic electrolytic or alkaline electrolyzed water The processed ginseng according to claim 1, wherein the ginseng is treated with acidic electrolytic water or alkaline electrolyzed water at a temperature of 50 to 80 ° C, and the additionally treated ginseng is grown for 0.5 to 15 hours at a temperature of 90 to 120 ° C. . The processed ginseng according to claim 1, which is obtained by treating acidic electrolyzed water or alkaline electrolyzed water in a weight ratio of 5 to 100 times with respect to ginseng. The processed ginseng according to any one of claims 1 to 3, wherein the content of Rg2, Rg3 and Rg5 is increased by 5 to 50 times compared to the raw material. The processed ginseng extract of the processed ginseng of any one of claims 1 to 3 extracted with water or C _1-4 alcohol or a mixed solvent thereof. Processed ginseng extract obtained by treating ginseng water or C _1-4 alcohol or a mixed solvent extract thereof with acid electrolytic water or alkaline electrolytic water in a weight ratio of 5 to 100 times at a temperature of 50 to 100 ° C. and drying under reduced pressure. The processed ginseng or its extract according to any one of claims 1 to 3, 5 or 6, further comprising red ginseng or white ginseng powder, an extract thereof, or a lyophilisate thereof. 8. The processed ginseng or extract thereof according to claim 7, comprising red ginseng or white ginseng powder, extract thereof, or lyophilized product thereof in a ratio of 0.01 to 100 based on the weight of the processed ginseng or extract thereof. The method according to claim 1 to 3, 5 or 6, wherein the ginseng is Panax ginseng , P. japonicum , P. quinquefolium , Panax notoginseng Processed ginseng or extracts thereof characterized by using the root and / or ground of one or more plants selected from the group consisting of P. notoginseng , P. trifolium and P. pseudoginsen g .

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