CN105748587A - Production process and method for extracting medicinal herb sculellaria barbata and the like - Google Patents
Production process and method for extracting medicinal herb sculellaria barbata and the like Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention discloses a process extracting herbal medicine sculellaria barbata. The process comprises the following steps of: A, conventionally washing the herbal medicine sculellaria barbata, airing for later use; B, grinding the backup sculellaria barbata to a fineness of 150-350meshes, mixing with artificially deep electrolysed alkaline water which is about 5 times of the weight of sculellaria barbata to generate grinding solution; C, adding artificially deep electrolysed alkaline water which is about 2 times of the weight of sculellaria barbata into the grinding solution, heating to 50-100 DEG C to cook for about 0.5-2.5 hours; D, filtering to generate a sculellaria barbata extracted filtrate; and E, cooling the filtrate to 10-25 DEG C, adding protease, cellulase and amylase, stirring for 2 hours, regulating the pH value of the filtrate to 2.5-7.0 with artificially deep electrolysed acidic water, adding new artificially deep electrolysed acidic water every standing 1 hour to regulate the pH value of the sculellaria barbata filtrate, and keeping the pH value of the filtrate to be 2.5-7.0 for totally 20-30 hours; and G, centrifugally dehydrating the sculellaria barbata filtrate, drying the precipitate, and taking out dry powder for preparing a sculellaria barbata anti-tumor herbal medicine.
Description
Technical field
The present invention relates to the extraction preparation method of a kind of autonomic drug, the invention belongs to pharmaceutical technology field, relate to a kind of Sculellaria barbata and the preparation method of other autonomic drugs extraction.Biological method especially with artificial deep electrolytic alkalescence with acid water is prepared and extraction process and the method using plant.
Background technology
Sculellaria barbata (Scutellaria barbata) is as conventional Chinese medicine or autonomic drug, there is many-sided effect such as heat-clearing, removing toxic substances, wide clinical application is in multi-infection diseases such as treatment pharyngitis, tonsillitis, pneumonia, mastitis, enteritis, dysentery, boils, and ephritis, hepatitis, cirrhotic ascites, venomous snake bite, with the treatment of Several Kinds of Malignancy.Modern pharmacological research shows, crude extract and effectively composite parts, the effective monomer component thereof of Sculellaria barbata have many pharmacologically actives such as sudden change antibacterial, anti-, anti-inflammatory, antipyretic, anti-oxidant, antitumor, immunological regulation.In recent years, medical value and the clinical practice thereof of Sculellaria barbata are paid attention to the most widely, country's State Key Task 95 problem the quality standard research of the Sculellaria barbata completed has taken in the version Pharmacopoeia of the People's Republic of China in 2000.But, up to now, the minority kind such as Sculellaria barbata sheet that preparation relevant with Sculellaria barbata on domestic medical market is only used as medicine with herb or crude extract, blood stasls syndrome, Reyanning sheet (capsule).Data-searching, at present, both at home and abroad other unit not yet have that Sculellaria barbata flavone component is antiviral, antibacterial, anti-inflammatory, antipyretic, analgesia comprehensive drug and new drug development product.
Tumour, particularly malignant tumour, be a kind of disease to human life with substantial risk.Medical domain is being studied always, is preventing, is being treated neoplastic disease the most in the world, such as chemical synthetic drug, chemotherapy, operation, ray radiotherapy etc. are used, although there is certain curative effect, but its therapy there is also many toxicity, side effects, wherein the toxicity of chemical synthetic drug is relatively strong, and some vitals of human body are had serious detrimental effect；Chemotherapy, ray radiotherapy, also murder the normal cell of human body while murdering cancer cell；For late malignant tumour, operation can accelerate the diffusion of cancer cell in some cases.
Motherland's medical science has particularly research to antitumor, and the celestial being that Jiang Yi " medicine mirror pick up any lost article from the road taxs " records Sculellaria barbata solution snakebite is careless；Among the people wipe out and treat insect pests and plant diseases outside venomous snake bite, still with curing oncoma, effective in cure；" conventional Chinese medicine planting technology " page 290 (Yao Zongfan, yellow heroic bearing are write, Golden Shield publishing house publishes, always issues, the 10th printing January in 1998) make to introduce to " Sculellaria barbata " in Chinese herbal medicine: Sculellaria barbata, another name: narrow leaf indian skullcap herb with root, toothbrush grass head grass, Herba Scutellariae Darbatae etc.；Section belongs to: for Labiatae Scutellaria plant；General introduction: Sculellaria barbata, with herb hyoscine, has clearing heat and detoxicating, promoting blood circulation, swelling and pain relieving and anticancer function, cures mainly tumour, hepatitis, Ascites Complicating Liver Cirrhosis, lung abscess etc. and levies, especially to effects such as lung cancer, cancer of the stomach, the cancer of the esophagus, cervical carcinomas preferably.But decocts owing to the general compatibility of Chinese traditional herbs uses, concentrate, extract, spray, be dried, sweet refining etc. carries out soup clothes and treats, and in soup, impurity and active ingredient are mixed, and cause taking effect slowly of clothes for patients.Meanwhile, the taste of decoction of medicinal ingredients also makes some patients inspire awe even from distance.
Research shows to be found to have 88 kinds of Chinese medicines inhibiting rate to tumor cell proliferation when 800 kinds of Chinese medicines being made antitumor activity screening by experiment in vitro more than 90%, wherein Sculellaria barbata reaches 100% to JTc-26 tumour cell extracorporeal inhibiting rate, and it is then only 50% to Normocellular inhibiting rate.The major physiological active material of Sculellaria barbata is flavone compound, and modern pharmacology research shows, extract from Scutellaria barbatae D has obvious inhibitory action to A549, HL-60, HONE1, KB, HT29, U937 type tumour cell.
Sculellaria barbata herb contains multiple flavones, and what its flavones reported by document mainly includes scutellarin, scutellarin, cyanidenon and apiolin etc., the most referred to as rivularin.Wherein obvious with cyanidenon and apiolin antitumor activity, have wide practical use.In Sculellaria barbata, principle active component is flavone compound, and what its content was most is scutellarin.In Sculellaria barbata, the ultra-violet absorption spectrum of extractive of general flavone and the ultra-violet absorption spectrum of scutellarin are basically identical, are respectively arranged with a maximum absorption band at 285 nm and 335 nm.And at 335 nm, the UV absorption of general flavone is affected less by other composition in Sculellaria barbata.Flavonoid substances in Sculellaria barbata, No. CAS: 80713-32-2；Molecular formula=C16H12O6；Molecular weight 300. 27；Molecular structural formula: physicochemical property: faint yellow needle (methyl alcohol) mp.
2780C (decomposes), Mg-HCl reacting positive.
What the at present extraction of Sculellaria barbata was concentrated mainly on general flavone is extracted as main, and common extracting method has solvent extraction method, microwave loss mechanisms and supercritical fluid extraction.The water extraction of Sculellaria barbata is suitable to flavonoid glycosides material and extracts.This method low cost, simple to environment and mankind's nonhazardous, equipment, it is suitable for industrialized production, but recovery rate is low,
Impurity more (such as inorganic salts, protein, carbohydrate etc.) in extract, later separation bothers,
Present little single use the method.The microwave extract method of Sculellaria barbata, through assay, this extracting method can only obtain crude extract.Sculellaria barbata Solvent Extract methods process has many kinds, as used ethanol water extracting method.According to the flavone compound organic solvent that select be suitable for different from impurity polarity,
The mixed solvent that conventional ethyl acetate, acetone, ethanol, methyl alcohol, water or some polarity are bigger extracts.All these Solvent Extract methods processes, have extractive technique complicated, and cost is high, active ingredient purity is low, but impurity is more,
It is unfavorable for purifying.Some flavone compound can be combined into insoluble substance with organic solution and is filtered out simultaneously.It is additionally useful for the organic-inorganic solvent of plant extraction or has certain toxicity or have plant extract component pollution and preparation process also to have severe contamination to environment.Sculellaria barbata can also be extracted by supercritical fluid technique.Supercritical fluid extraction
(SFE) refer mainly to overcritical
CO2 extracts, and this method recovery rate is high, noresidue solvent contamination.But this method production cost is high,
In extract, alkyl phenol content is higher, and supercritical CO 2 is non-polar solven,
Nonpolar and that molecular weight is the lowest polar substances is shown good dissolubility,
But the material solvability stronger to polarity is not enough.Its solvability can be made to improve although increasing density,
But increasing density and need to improve extracting pressure, the expense making extraction equipment is dramatically increased by this, is unsuitable for large-scale production.
Summary of the invention
The invention aims to overcome above-mentioned existing medicinal plant extractive technique, the particularly defect of the extraction extractive technique of Sculellaria barbata etc. and deficiency, operation cost is low, recovery rate is higher, to plant extract medicine product noresidue solvent contamination, the extraction process of environmentally safe and method to provide one.
A kind of extraction process based on artificial ionization's alkalescence with the autonomic drug Sculellaria barbata of acid water, it is characterised in that comprising the steps of this operating procedure is: A. a certain amount of autonomic drug Sculellaria barbata, carries out conventional cleaning in stainless steel tank, and taking-up is dried standby；B., standby Sculellaria barbata is put into blade vertical shaft micro-nano pulverizer pulverize, smashing fineness 150 mesh 350 mesh, add the manual depth for Sculellaria barbata weight about 5 times and be electrolysed alkaline water (pH value=8-11) mixing, produce and pulverize liquid；C. pulverizing liquid is put in stainless-steel pan, add the manual depth for Sculellaria barbata weight about 2 times and be electrolysed alkaline water (pH value 8-11), be heated up to 50-100 DEG C and boil about 0.5-2.5 hour；The most then add filter cloth bag at blowing valve port, be then turned on baiting valve and filter, produce Sculellaria barbata and extract filtered fluid；E. in aseptic temperature control workshop, filtered fluid is poured in the stainless-steel pan with blender apparatus, it is cooled to 10-25 DEG C, add a certain amount of protease, cellulase and amylase, it is stirred under conditions of rotating speed 30-80 rev/min 2 hours, and constantly employment work deep electrolytic acid water regulates the pH value of filtered fluid to 2.5-7.0；F. can be continually changing in atmospheric environment due to the pH value of manual depth's electrolyzed acidic water, the most static 1 hour of the filtered fluid of addition artificial ionization's acid water, add the pH value of new manual depth's electrolyzed acidic water regulation Sculellaria barbata filtered fluid to 2.5
-7.0；Maintaining filtered fluid is 20-30 hour in the total time of pH value to 2.5-7.0 stages；G. in aseptic temperature control workshop, static Sculellaria barbata filtered fluid is put into centrifugal dehydrator carry out dehydration separate after, produced sediment is put into setting constant temperature 40-60 DEG C in electric drying oven with forced convection and is dried drying, and the dry powder of taking-up can be used for the preparation of Sculellaria barbata anti-tumor botanical.
In the extraction process of described autonomic drug Sculellaria barbata medicine and method, can be with addition for the 0. of Sculellaria barbata weight
The protease of 1-0.9%.
In the extraction process of described Sculellaria barbata medicine, can be with addition for the 0. of Sculellaria barbata weight
The cellulase of 1-0.9%.
In the extraction process of described Sculellaria barbata medicine, can be with addition for the 0. of Sculellaria barbata weight
The amylase of 1-0.9%.
In the extraction process of described Sculellaria barbata medicine, manual depth is electrolysed alkalescence acid water and is formed by the artificial electrolytic ionization of natural water added electric field.Its pH value is determined with the time by the size of extra electric field, direction.In atmospheric environment, the pH value of manual depth's electrolyzed acidic water can be continually changing, and its alkalescence acid degree constantly reduces, and tends to neutral.
In the extraction process of described Sculellaria barbata medicine, artificial ionization's acid water regulation Sculellaria barbata is used to extract the pH value of filtered fluid, its pH value range: 2.5-7.0 Sculellaria barbatas extract the different adjustment pH value of filtered fluid will cause the rivularin or the Flavonoid substances that finally obtain heterogeneity.
In the extraction process of described Sculellaria barbata medicine, add artificial ionization's alkaline water and produce Sculellaria barbata extraction pulverizing liquid, its pH value range: 8.0-11.0. add the manual depth of different pH value and are electrolysed alkaline water, will cause the rivularin or the Flavonoid substances that finally obtain heterogeneity.
In the extraction process of described Sculellaria barbata medicine, Sculellaria barbata is pulverized liquid and puts in stainless-steel pan, add the artificial deep electrolytic alkaline water (pH value 8-11) for Sculellaria barbata weight 2 times, it is heated up to 50-100 DEG C boil 1.0 hours, adds in warm and humid boiling every 0.2 hour and add the artificial ionization alkaline water (pH value 8-11) into Sculellaria barbata weight 2 times；The warm and humid temperature and time that boils of different height will cause finally obtaining rivularin or the Flavonoid substances of heterogeneity.
Different pH value, different wet boil temperature, different wet boil the time and will cause finally obtaining rivularin or the Flavonoid substances of heterogeneity.The artificial deep electrolytic ionization alkaline water of different pH value can be used to extract Flavonoid substances according to different raw materials and the last required requirement obtaining composition.General it should be noted that Acidity of Aikalinity is unsuitable too strong, in order to avoid highly basic destroys flavones when heating, it is also prevented from when acidifying generating salt simultaneously, makes the flavones double decomposition again of precipitation, affect recovery rate.General free glucoside unit, indissoluble is dissolved in the artificial deep electrolytic ionization alkaline water of low pH value, it is soluble in the artificial deep electrolytic alkaline water liquid of high pH value, flavonoid glycosides is soluble in the artificial deep electrolytic ionization basic aqueous solvent of low pH value, therefore artificial ionization's basic aqueous solvent of high pH value suitably extracts flavones and dissociates glucoside unit, the artificial deep electrolytic of low pH value ionizes in alkaline water liquid and suitably extracts flavonoid glycosides.
The invention have the advantage that
1. operation sequence is few, and device therefor is conventional equipment on market, and art production process is relatively easy thus
2. the Barbat skullcap general flavone preparation that the present invention obtains is pure medicinal plant preparation, has the selection of material scientific and reasonable, and preparation technology is the most advanced, economical and practical, the advantage being suitable for industrialized production
The most artificial deep electrolytic alkaline water extracts medicinal plant Sculellaria barbata etc., and it is high that it refines active ingredient.Carrying out operation according to present invention process method step, the 6-10 % that antineoplastic dry powder is Sculellaria barbata input amount of acquisition, active ingredient extraction is high thus；
4. flavone compound has phenolic hydroxyl group mostly, is soluble in buck, the most precipitable precipitation after acidifying.Its reason one is due to the acidity of flavones phenolic hydroxyl group, and two is due to the open loop in the basic conditions of flavones parent nucleus, forms hydroxy chalcone, and polarity increases and dissolves.Therefore with artificial deep electrolytic alkaline water, rivularin can be leached, after leachate is acidified, separate out flavone compound.The pH value of artificial deep electrolytic alkaline water can arbitrarily regulate with temperature, and the ability that therefore leaches is high.This is the great innovation of plant extraction medicine in the world.
5. in Sculellaria barbata herb in addition to containing higher fatty acids and esters thereof, possibly together with substantial amounts of steroid.Compared with organic solvent extraction, the Sculellaria barbata extraction of artificial deep electrolytic ionization acid water shows the selectivity good to steroid, when extraction product is carried out antitumor activity screening, finds that steroid has obvious antitumor activity.By to artificial deep electrolytic ionization acid water acid degree and the optimization of extraction temperature, optimal Studies on Anticancer Components can be filtered out from Sculellaria barbata, develop for the anti-tumor Chinese medicine compound containing Sculellaria barbata and antineoplastic and provide certain theoretical foundation.
The selection of material the most of the present invention is scientific and reasonable, and preparation technology is the most advanced, economical and practical, is suitable for industrialized production.Owing in Barbat skullcap general flavone, major part is flavonoid glycoside, a small amount of or without flavonoid glycoside metaclass, it is highly soluble in alkaline solution.Consider the flavone compound unstability to soda acid simultaneously.Therefore present invention uses a kind of unprecedented effective extracting method, using artificial deep electrolytic acid water with artificial deep electrolytic alkaline water as general flavone Extraction solvent.
Barbat skullcap general flavone extract has strong suction-operated through experimental verification, activated carbon to flavones ingredient in Sculellaria barbata, so not selecting activated carbon to process in preparation technology.
The present invention is by the extraction process of artificial deep electrolytic ionization acid water with artificial deep electrolytic ionization alkaline water, obtain highly purified Herba Scutellariae Barbatae extract, technique is simple, environmental friendliness, does not use any organic solvent, low production cost, is suitable to large-scale industrial production.
Having separated at present six chemical compositions that Sculellaria barbata obtains is carthamin (carthamidin), isocarthamidin (isocarthamidin), scutellarin (having another name called scutellarin) (scutellarin), cupreol (β-sitosterol), stearic acid (stearicacid) and alkaloid (alkaloid).It addition, also isolated has antineoplastic active component Scutellaria Barbata D. Don Polysaccharides (SBP) and a kind of infected by influenza has material--the trihydroxy-8-methoxy flavone of inhibitory action from Sculellaria barbata.The present invention extraction process by artificial deep electrolytic acid water and artificial deep electrolytic alkaline water, all in Sculellaria barbata can be separated it has been reported that chemical composition and other composition.
Detailed description of the invention:
Embodiment 1
By medicinal Sculellaria barbata, carrying out conventional cleaning in stainless steel tank, taking-up is dried, and pulverizes；
The Sculellaria barbata of pulverizing is put into nano grinder pulverize, smashing fineness 150 mesh-350 mesh, add the artificial deep electrolytic alkaline water (pH value 8-11) that adds for Sculellaria barbata weight 5 times and mix, produce and pulverize liquid；
Again pulverizing liquid is put in stainless-steel pan, and be incorporated as the artificial deep electrolytic alkaline water (pH value 8-11) of Sculellaria barbata weight 2 times, be heated up to 50-100 DEG C and boil about 0.5-2.5 hour；
During pulverizing liquid adds warm and humid boiling, within every about 0.5 hour, add as artificial deep electrolytic alkaline water (pH value 8-11)；
Then add filter cloth bag at blowing valve port, be then turned on baiting valve and filter, produce Sculellaria barbata and extract filtered fluid；
In aseptic temperature control workshop, filtered fluid is poured in the stainless-steel pan with blender apparatus, it is cooled to 10-25 DEG C, add a certain amount of protease, cellulase and amylase, it is stirred under conditions of rotating speed 30-80 rev/min 2 hours, and constantly employment makes the pH value of ionization acid water regulation filtered fluid to 2.5-7.0；
Owing to the pH value of manual depth's electrolyzed acidic water can be continually changing in atmospheric environment, add the filtered fluid of manual depth's electrolyzed acidic water after every static every 1 hour；Add the pH value of new high ph-values artificial deep electrolytic acid water regulation Sculellaria barbata filtered fluid again to 2.5-7.0；Filtered fluid is 20-30 hour in the total time of pH value to 2.5-7.0 stages；
In aseptic temperature control workshop, static Sculellaria barbata filtered fluid liquid is put into centrifugal dehydrator carry out dehydration separate after, produced sediment puts into setting constant temperature 40-60 DEG C in electric drying oven with forced convection, and vacuum drying obtains the extract of Sculellaria barbata flavonoids anti-tumor active ingredient.
Embodiment 2
Prepare granule and tablet:
The embodiment 1 gained Barbat skullcap general flavone extract of 15%-55% is weighed based on configuration total amount, add the Sculellaria barbata nano powder of 45%-85%, it is thoroughly mixed all, binder is made with 70% (V/V) ethanol, make softwood, cross 14 mesh nylon screens and make particle, (this wet granular directly can be rolled in coating pan micropill, without adding any auxiliary material), wet granular is dried 23 hours in 60 ° of C, dry particle crosses the 14 whole grains of mesh sieve, makes every gram of particle 600mg Han Barbat skullcap general flavone extract 200, prepares oral liquid or mixture.
This dry particle is direct, or add medicinal plant formation compound, it is prepared as tablet or soft capsule.
Claims (8)
1. an extraction process based on artificial ionization's alkalescence with the autonomic drug Sculellaria barbata of acid water, it is characterised in that comprising the steps of this operating procedure is: A. a certain amount of autonomic drug Sculellaria barbata, carries out conventional cleaning in stainless steel tank, and taking-up is dried standby；B., standby Sculellaria barbata is put into blade vertical shaft micro-nano pulverizer pulverize, smashing fineness 150 mesh 350 mesh, add the manual depth for Sculellaria barbata weight about 5 times and be electrolysed alkaline water (pH value=8-11) mixing, produce and pulverize liquid；C. pulverizing liquid is put in stainless-steel pan, add the manual depth for Sculellaria barbata weight about 2 times and be electrolysed alkaline water (pH value 8-11), be heated up to 50-100 DEG C and boil about 0.5-2.5 hour；The most then add filter cloth bag at blowing valve port, be then turned on baiting valve and filter, produce Sculellaria barbata and extract filtered fluid；E. in aseptic temperature control workshop, filtered fluid is poured in the stainless-steel pan with blender apparatus, it is cooled to 10-25 DEG C, add a certain amount of protease, cellulase and amylase, it is stirred under conditions of rotating speed 30-80 rev/min 2 hours, and constantly employment work deep electrolytic acid water regulates the pH value of filtered fluid to 2.5-7.0；F. can be continually changing in atmospheric environment due to the pH value of manual depth's electrolyzed acidic water, the most static 1 hour of the filtered fluid of addition artificial ionization's acid water, add the pH value of new manual depth's electrolyzed acidic water regulation Sculellaria barbata filtered fluid to 2.5-7.0；Maintaining filtered fluid is 20-30 hour in the total time of pH value to 2.5-7.0 stages；G. in aseptic temperature control workshop, static Sculellaria barbata filtered fluid is put into centrifugal dehydrator carry out dehydration separate after, produced sediment is put into setting constant temperature 40-60 DEG C in electric drying oven with forced convection and is dried drying, and the dry powder of taking-up can be used for the preparation of Sculellaria barbata anti-tumor botanical.
In the extraction process of autonomic drug Sculellaria barbata medicine the most according to claim 1 and method, can be with addition for the 0. of Sculellaria barbata weight
The protease of 1-0.9%.
In the extraction process of Sculellaria barbata medicine the most according to claim 1, the cellulase of 0. 1-0.9% that can be Sculellaria barbata weight with addition.
In the extraction process of Sculellaria barbata medicine the most according to claim 1, the amylase of 0. 1-0.9% that can be Sculellaria barbata weight with addition.
In the extraction process of Sculellaria barbata medicine the most according to claim 1, manual depth is electrolysed alkalescence acid water and is formed by the artificial electrolytic ionization of natural water added electric field, its pH value is by the size of extra electric field, direction determined with the time, in atmospheric environment, the pH value of manual depth's electrolyzed acidic water can be continually changing, its alkalescence acid degree constantly reduces, and tends to neutral.
In the extraction process of Sculellaria barbata medicine the most according to claim 1, artificial ionization's acid water regulation Sculellaria barbata is used to extract the pH value of filtered fluid, its pH value range: 2.5-7.0 Sculellaria barbatas extract the different adjustment pH value of filtered fluid will cause the rivularin or the Flavonoid substances that finally obtain heterogeneity.
In the extraction process of Sculellaria barbata medicine the most according to claim 1, add artificial ionization's alkaline water and produce Sculellaria barbata extraction pulverizing liquid, its pH value range: 8.0-11.0 manual depth adding different pH value are electrolysed alkaline water, will cause the rivularin or the Flavonoid substances that finally obtain heterogeneity.
In the extraction process of Sculellaria barbata medicine the most according to claim 1, Sculellaria barbata is pulverized liquid and puts in stainless-steel pan, add the artificial deep electrolytic alkaline water (pH value 8-11) for Sculellaria barbata weight 2 times, it is heated up to 50-100 DEG C boil 1.0 hours, adds in warm and humid boiling every 0.2 hour and add the artificial ionization alkaline water (pH value 8-11) into Sculellaria barbata weight 2 times；The warm and humid temperature and time that boils of different height will cause finally obtaining rivularin or the Flavonoid substances of heterogeneity.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060008020A (en) * | 2004-07-23 | 2006-01-26 | 주식회사 유니젠 | Processed ginseng |
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2016
- 2016-03-01 CN CN201610114018.3A patent/CN105748587A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060008020A (en) * | 2004-07-23 | 2006-01-26 | 주식회사 유니젠 | Processed ginseng |
Non-Patent Citations (1)
| Title |
|---|
| 郭雪蜂 等: "黄酮类化合物的提取•分离纯化和含量测定方法的研究进展", 《安徽农业科学》 * |
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