KR20050122074A - Cosmetic composition comprising torreya nucifera extract as active ingredient for improving skin wrinkle and elasticity - Google Patents

Abstract

The present invention provides a composition for promoting elastase inhibition and collagen synthesis and a composition for improving skin wrinkles and elasticity comprising a visa extract as an active ingredient. Visa extract of the present invention has excellent elastase inhibition, collagen synthesis promoting and fibroblast proliferation effect, in particular the water fraction of the Visa extract has excellent elastase inhibitory activity. Cosmetic composition comprising a non-extract extract of the present invention as an active ingredient is excellent in improving the skin wrinkles and skin elasticity, and exhibits excellent stability.

Description

Cosmetic composition for improving skin wrinkles and elasticity comprising a non-extract extract as an active ingredient {Cosmetic Composition Comprising Torreya nucifera Extract as Active Ingredient for Improving Skin Wrinkle and Elasticity}

The present invention relates to a composition comprising a visa extract, and more particularly, the present invention relates to a composition for inhibiting elastase, a composition for promoting collagen synthesis, and a cosmetic composition for improving skin wrinkles and elasticity, comprising a visa extract as an active ingredient. It is about.

Skin is an organ that covers the body's surface. The skin is largely divided into epidermis and dermis. The epidermis is the outermost of the skin and acts as a protective barrier for internal organs. The epidermis is composed of a stratum corneum, a clear layer, a granule layer, a play layer and a base layer. The dermis serves to nourish the epidermis and form the skin's framework within the skin tissue. The dermis is composed of a papillary layer and a deep reticular layer, which is a place where skin cell proliferation is induced by fibroblast activity. The reticular layer plays a role in maintaining the elasticity of the entire skin, and collagen (collagen) and elastin (elastic fiber) are meshed (Braverman, J. Invest. Dermatol. , 78, 434-443 (1982). )).

Elastic fibers are elastic fibers included in connective tissue and are much more stretchable than collagen fibers included in connective tissue. Elastic fibers provide elasticity to the skin so that the deformed skin can be restored to its original shape by the force applied to the skin.

Elastin is the main component of the elastic fiber is composed of elastin (90%) of the amorphous insoluble protein 90% of the elastic fiber. Elastase is an enzyme that degrades elastin, and the breakdown of elastin by elastase decreases the elasticity of the skin and accelerates the production of wrinkles (Dermatology pp 18, Korean Dermatology Textbook). Committee compilation, Ryeomungak)

Therefore, a method of inhibiting the degradation of elastin by inhibiting the activity of elastase as a method for improving skin wrinkles and elasticity has been reported (Kim In-young et al . , Kor. J. Pharmacogn 33 (4): 364-371 (2002)).

Collagen is an important protein that accounts for 30% of the total weight of biological proteins and has a solid triple helix structure. The main functions are known as mechanical firmness of skin, resistance of connective tissue and tissue binding, and support of cell adhesion (Van der Rest et al, 1990). Such collagen is deteriorated in the function of fibroblasts by photoaging due to aging and ultraviolet irradiation, and the production amount thereof is reduced. In general, at 80 years of age, the skin thickness becomes thinner by 65% compared to 20 years, and this phenomenon is known to be closely related to the wrinkle formation of the skin (Arthur K. Balin et al., "Aging and the skin" ", 1989). Recently, cosmetic products containing collagen have been released, but these cosmetics are applied to the skin surface, and it is difficult to absorb the collagen, a polymer, percutaneously. .

Wrinkle improvement materials, such as retinoids used in the prior art has a low stability, there is a limit to use in cosmetics, irritation and redness occurs during application of the skin, there is also a safety problem, the amount of use is limited.

Therefore, there is a need for development of a new material having excellent stability and safety as well as an effect of improving wrinkles and elasticity.

Throughout this specification, a number of articles are referenced and their citations are indicated. The disclosures of cited articles are incorporated herein by reference in their entirety to more clearly describe the level of the technical field to which the present invention pertains and the content of the present invention.

The present inventors have conducted extensive research on herbal medicines to find a material having high stability and excellent skin wrinkle and elasticity improvement. As a result, Visa extract has excellent elastase inhibitory activity, collagen synthesis promotion, and fibroblast proliferation effect. Confirming the superiority, the present invention was completed.

Accordingly, it is an object of the present invention to provide a composition for inhibiting elastase comprising a non-extract extract as an active ingredient.

An object of the present invention to provide a composition for promoting collagen synthesis comprising a non-extract extract as an active ingredient.

Another object of the present invention to provide a cosmetic composition for improving skin wrinkles and elasticity comprising a non-extract extract as an active ingredient.

According to one aspect of the invention, the present invention provides a composition for inhibiting elastase comprising a non-extract extract as an active ingredient.

According to another aspect of the invention, the present invention provides a composition for promoting collagen synthesis comprising a non-extract extract as an active ingredient.

According to another aspect of the present invention, the present invention provides a cosmetic composition for improving skin wrinkles and elasticity comprising a non-extract extract as an active ingredient.

The present invention is characterized by including a visa extract as an active ingredient.

Torreya nucifera is an evergreen conifer plant belonging to the Taxaceae family, and it is distributed only in Korea and Japan. Its growth is so slow that it can't be seen well. It is designated as a natural monument and protected in Korea. Viburnum is used for edible, ornamental, industrial, and medicinal. Seeds are squeezed and eaten oil. Wood is used for landscaping, intestinal bleeding, etc. Wood is used for building materials, machinery materials, and ship materials (Kim, Tae-Jung, Korea Resources Plant I, p 40, Seoul National University Press, 1996; ). The reported components isolated from leaves and seeds include sesquiterpenoids (Sakaki T, Nishimura K, Hirose Y., Bull. Chem. Soc. Japan, 38: 381-725, 1965), labdane Family and abietane diterpenoids (Sayama Y, Kyogoku K, Murayama H, Agric. Biol. Chem. 1971; 35: 1068-73 and Harrison LJ, Asakawa Y, Phytochemistry, 1987; 26 1211-2) and flavonoids (Kariyone T, Sawaka T, Yakugaku Zasshi, 1958; 78: 1010-3).

 As used herein, the term "visa extract" refers to an extract obtained from various organs (such as fruit, seeds, leaves, flowers, bark, stems and roots, etc.) of the non-tree, preferably means the seed extract of the non-tree do.

The visa extract used in the present invention may be obtained through various methods known in the art, and preferably includes (a) water, (b) anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (eg, methanol, ethanol). , Propanol, butanol and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol. The extraction solvent is preferably alcohol, more preferably ethanol or methanol. Visa used as the material for extraction can be dried or used as it is, and preferably it is dried. As a drying method, natural drying or a hot air drying method can be used.

Referring to the following specific preparation of the present invention, the preparation of the visa extract is described as follows: Visa is washed with purified water, dried and then ground. The ground non-pulverized fine powder is put in methanol or ethanol and leached for several days at room temperature. The leachate was recovered by filtering with Whatman filter paper, and re-leached with methanol or ethanol. The releach process is repeated several times and the recovered leachate is concentrated in a vacuum concentrator.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also a non-extract extract having substantially the same effect using other extraction solvent.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the non-extract of the present invention.

The non-extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

According to a preferred embodiment of the present invention, the non-extract extract used in the present invention, after the extraction using the above-mentioned extraction solvent, for the further fractionation of the active ingredient, water and organic solvents (such as hexane, ethyl acetate and Fractionation with chloroform). Thus, the extract of the present invention is more preferably a water layer fraction.

The present inventors measured the elastase inhibitory effect of the fractions of each solvent by fractionation of non-extracted extracts with various solvents, the results confirmed that the water layer fraction has an excellent elastase inhibitory effect.

From the above facts, it can be seen that the substance having high solubility in water in the non-extract extract of the present invention is particularly excellent in elastase inhibitory effect. In addition, fractionation of the non-extract by the solvent allows removal of unnecessary substances and the use of only essential components.

Referring to the following specific preparation of the present invention, the solvent-specific fraction of the extract was described as follows: The extract obtained using the extractant was dispersed in purified water and shaken by adding hexane. After the hexane layer and the water layer were sufficiently separated, an upper hexane layer was taken. Hexane was added to the lower layer (water layer) to extract several more times. After all the recovered upper layers were combined, the solvent was removed using a vacuum distillation apparatus. The lower layer (water layer) is reextracted and concentrated in the same manner as above using chloroform and ethyl acetate instead of hexane. Finally, the water layer is distilled under reduced pressure to obtain a water layer fraction.

Visa extract of the present invention has excellent elastase inhibitory effect and excellent collagen synthesis promoting effect.

The reticular layer, which plays a role of maintaining the elasticity of the entire skin, is composed of collagen (collagen fiber) and elastin (elastic fiber) in a net shape. The elasticity of the skin is greatly affected by the elastic fibers mainly composed of elastin. When the elastin of the elastic fibers is decomposed by the elastin degrading enzyme, the skin loses its elasticity and accelerates wrinkle formation. Visa extract of the present invention by inhibiting the elastase activity inhibits the breakdown of elastin by the elastin degrading enzyme so that the elastic fiber can perform its function smoothly. In addition, by promoting the mechanical strength of the skin, the resistance of connective tissue and the adhesion of tissue, the synthesis of collagen to support the cell adhesion, as a result, the elasticity of the skin is maintained and improved, and furthermore, the formation of wrinkles is suppressed and wrinkles are improved. Get

Moreover, the non-extract extract of the present invention promotes the proliferation of fibroblasts constituting the fibrous connective tissue of the dermis. The proliferative effect of the fibroblasts by the extract of the present invention enables the supply of healthy young cells to the fibrous connective tissues, thereby elastase-inhibiting activity of the non-extract extract, promoting collagen synthesis and consequently strengthening the fibrous connective tissues of the skin. Give effect.

According to a preferred embodiment of the present invention, the content of the non-extract extract is 0.0001 to 15% by weight, preferably 0.001 to 10% by weight and more preferably 0.01 to 5% by weight based on the total composition. If the amount of the non-extractable extract is less than 0.0001% by weight, there is a problem in that the effect does not appear.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to non-extract extracts as active ingredients, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, and Carrier.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Cosmetic composition of the present invention comprising a non-extract extract as an active ingredient not only inhibits the activity of the elastase and promotes the synthesis of collagen, but also promotes fibroblast proliferation to improve skin wrinkles and elasticity. In addition, the cosmetic composition containing the non-extract of the present invention exhibits excellent temperature stability.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Example

Preparation Example 1 Preparation of Visa Extract

Visarey (Torreyae semen) was washed with purified water, dried and then ground. Crushed Visa 140 g of fine powder was added to 1.4 L of methanol or ethanol and leached at room temperature for 1 day. The leachate was collected by filtration through Whatman No. 2 filter paper, and then 1.4 L of methanol or ethanol was added and releached. The releach process was repeated three times in total. The recovered leachate was concentrated under a reduced pressure concentrator, and 53.3 g of dry extract was obtained.

Preparation Example 2 Preparation of Fractions by Solvent

Obtained in Preparation Example 1 Methanol extract of Visa was dispersed in 1 L of purified water and shaken by adding 2 L of hexane. After the hexane layer and the water layer were sufficiently separated, an upper hexane layer was taken. Hexane was added to the lower layer (water layer) and extracted three more times. The combined upper layers were all combined, the solvent was removed using a vacuum distillation apparatus, and 67 g of hexane layer fractions were obtained. Using chloroform and ethyl acetate instead of hexane, the lower layer (water layer) was reextracted and concentrated in the same manner as above to obtain 60 mg of chloroform layer fractions and 115 mg of ethyl acetate layer fractions. Finally, the water layer was distilled under reduced pressure to obtain 4.91 g of a water layer fraction.

Experimental Example 1: Elastase inhibition assay

60 μl of a solution prepared for each concentration of the non-extract extract of Preparation Example 1 (solvent: 100 mM Tris HCl pH 8.0) and 100 μl of a 0.6 units / ml elastase enzyme (Sigma, United States) solution were reacted at 37 ° C. for 30 minutes. . Then, 40 μl of a 4 mM sucAla3-PNA solution (Sigma, USA) was added and reacted at 37 ° C. for 30 minutes, and then absorbance was measured at 405 nm using a spectrophotometer. The experimental results are shown in Table 1 below. Elastase inhibitory activity was calculated according to the following equation.

% Inhibition = {1- [comparative absorbance (O.D.405) / control absorbance (O.D.405)]] x 100

Visa extract (mg / ml) Elastase Inhibitory Activity (%) (Methanol Extraction) Elastase inhibitory activity (%) (ethanol extraction) 0.03 37.2 59.8 0.3 76.6 86.3 0.45 80.9 86.9

As shown in Table 1, the non-extract extract inhibited the activity of elastase in a concentration-dependent manner, and its inhibitory effect was excellent.

Experimental Example 2: Elastase inhibitory effect of the solvent-specific fractions of non-extract extract

The elastase inhibitory effect of the solvent-specific fractions of hexane, chloroform, ethyl acetate and water obtained in Preparation Example 2 was measured by the method described in Experimental Example 1.

Fraction by solvent (0.45 mg / mL) Elastase Inhibitory Activity (%) Hexane layer 7.7 Chloroform layer 5.7 Ethyl acetate layer 8.2 Water layer 56.6

As can be seen in Table 2, the water layer showed superior elastase inhibitory activity compared to the other layers. Therefore, it can be expected that a substance having high elastase inhibitory activity exists in the water layer.

Experimental Example 3: Collagen Synthesis Promoting Effect Experiment

Fibroblasts 1.4 x 10 ⁵ in each well of a 12-well plate Cells / well were seeded and incubated in DMEM medium for 24 hours. The non-extract extracts obtained using methanol of Preparation Example 1 were treated to 0.001, 0.01, 0.1 and 1 mg / ml, respectively, and incubated for 24 hours. The amount of collagen newly synthesized by fibroblasts was measured using a Sircol ^™ collagen assay kit (Biocolor Ltd., Ireland). That is, the amount of collagen synthesized was measured by measuring the absorbance at 540 nm using a spectrometer for the color reaction of the dye reagent specifically bound to the [Gly-XY] n triple helix structure found in collagen.

Visa extract (mg / ml) Collagen synthesis rate (%) 0.001 105.4 0.01 113.5 0.1 121.6 One 135.8

As shown in Table 3, it can be seen that the collagen synthesis rate is increased when the non-extract extract is treated in a concentration-dependent manner. Therefore, it was found that Visa extract was also excellent in promoting collagen synthesis.

Experimental Example 4: Fibroblast Proliferation Effect Experiment

4-1: Primary Cell Culture of Rat Fibroblasts

SD rats (4 weeks old) purchased from BIO GEOMENIX were anesthetized, their backs were removed, and tissues were extracted. After removing the epidermis and the dermis from the extracted tissue, fibroblasts were isolated from the dermis to confirm the morphology. The identified fibroblasts were stabilized at 37 ° C. for 3-5 days with 5% CO ₂ concentration in the incubator in a 25 cm 2 tissue culture flask by adding 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin to DMEM medium. It was. After stabilization, passages were treated with trypsin-EDTA solution and initial passage cells (passage 3) were used.

4-2: Fibroblast Proliferation Rate Measurement

Normal rat fibroblasts of the multiplier 4 x 10 ⁴ in each well of a 96-well plate Cells / well were seeded and incubated in DMEM medium for 24 hours. Methanol of Preparation Example 1 was exchanged with DMEM medium containing 5% serum treated so that the final concentration of the extract of Visa obtained 0.1, 0.5 and 1 mg / ㎖, respectively, and incubated for 72 hours. 50 μl (5 mg / ml) of the MTT solution was added and centrifuged after 4 hours to remove the supernatant. After 100 μl of DMSO was added, the absorbance was measured with an ELISA reader at 570 nm. Cell growth rate (%) was calculated according to the following equation (2).

% Cell proliferation = (absorbance of extract treated group-absorbance of control group) / absorbance of control group x 100

Visa extract (mg / ml) Cell proliferation effect (%) 0.1 108.1 0.5 122.8 One 92.4

As shown in Table 4, it can be seen that the visa extract has a cell proliferation effect of normal rat fibroblasts. When the concentration of the extract was 0.5 ㎎ / ㎖ brought the highest proliferative effect, and showed an excellent effect overall, it can be seen that the Visa extract has an excellent cell proliferation effect.

Hereinafter, examples of the formulation of the present invention include a flexible lotion, a nutrient lotion and a nourishing cream, but the formulation of the cosmetic including the composition of the present invention is not limited thereto.

Formulation example

Formulation Example 1

Formulation examples of the lotion (skin) in the cosmetic containing the non-extract extract is shown in Table 5.

number Raw material name Example 1 (% by weight) Example 2 (% by weight) Example 3 (% by weight) Comparative Example 1 (% by weight) One  Visa extract 0.01 1.00 5.00 - 2  glycerin 2.00 2.00 2.00 2.00 3  Butylene Glycol 3.00 3.00 3.00 3.00 4  Dipropylene glycol 2.00 2.00 2.00 2.00 5  EDTA-2Na 0.01 0.01 0.01 0.01 6  Pigment a very small amount a very small amount a very small amount a very small amount 7  Ethanol (95%) 5.00 5.00 5.00 5.00 8  antiseptic a very small amount a very small amount a very small amount a very small amount 9  Octyldodeceth-16 0.10 0.10 0.10 0.10 10  incense a very small amount a very small amount a very small amount a very small amount 11  Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Formulation Example 2

Formulation examples of the nutrient lotion in the cosmetic containing the non-extract extract are shown in Table 6.

number Raw material name Example 4 (wt%) Example 5 (% by weight) Example 6 (% by weight) Comparative Example 2 (% by weight) One  Visa extract 0.01 1.00 5.00 - 2  Lipophilic glyceryl stearate 1.50 1.50 1.50 1.50 3  Stearic acid 0.30 0.30 0.30 0.30 4  Stearyl alcohol 0.50 0.50 0.50 0.50 5  Beeswax 0.40 0.40 0.40 0.40 6  Polysorbate 60 1.20 1.20 1.20 1.20 7  Sorbitan sesquioleate 0.30 0.30 0.30 0.30 8  antiseptic a very small amount a very small amount a very small amount a very small amount 9  Squalane 3.00 3.00 3.00 3.00 10  Cetylethylhexanoate 5.00 5.00 5.00 5.00 11  Cyclomethicone 4.00 4.00 4.00 4.00 12  glycerin 3.00 3.00 3.00 3.00 13  Butylene Glycol 3.00 3.00 3.00 3.00 14  EDTA-2Na 0.03 0.03 0.03 0.03 15  Triethanolamine 0.15 0.15 0.15 0.15 16  Carbomer 0.12 0.12 0.12 0.12 17  Pigment a very small amount a very small amount a very small amount a very small amount 18  incense a very small amount a very small amount a very small amount a very small amount 19  Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Formulation Example 3

Table 7 shows an example of the formulation of the nutrition cream in the cosmetic containing the non-extract extract.

Number Raw material weight% One Visa extract 10.0 2 Beeswax 3.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 20.0 6 Sorbitan stearate 1.0 7 Lipophilic Monostearate Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 6.0 11 Triethanolamine 0.2 12 antiseptic a very small amount 13 Pigment a very small amount 14 Spices a very small amount 15 Purified water Remaining amount

Formulation Example 4

Formulation example of the massage cream in the cosmetic containing the non-extract extract is shown in Table 8.

Number Raw material weight% One Visa extract 10.0 2 Beeswax 5.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.8 5 Liquid paraffin 40.0 6 Squalane 5.0 7 Lipophilic Monostearic Acid Glycerin 1.0 8 Carboxy Vinyl Polymer 0.2 9 Butylene glycol 6.0 10 glycerin 6.0 11 Triethanolamine 0.2 12 antiseptic a very small amount 13 Pigment a very small amount 14 Spices a very small amount 15 Purified water Remaining amount

Formulation Example 5

Formulation examples of the nutritional essence in the cosmetic containing the non-extract extract are shown in Table 9.

Number Raw material weight% One Visa extract 5.0 2 Cytostolol 13.0 3 Polyglyceryl 2-oleate 0.2 4 Ceramide 0.1 5 Ceteares-4 5.0 6 cholesterol 0.3 7 Dicetylphosphate a very small amount 8 Concentrated glycerin 1.0 9 Macadamia oil 0.2 10 Carboxy Vinyl Polymer a very small amount 11 Xanthan Gum a very small amount 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount

Formulation Example 6

Examples of the formulation of the emulsion-based foundation in the cosmetic containing the non-extract extract are shown in Table 10.

Number Raw material weight% One Visa extract 10.0 2 Beeswax 2.0 3 Pyromethicone 2.0 4 Liquid paraffin 5.0 5 Squalane 5.0 6 Stearic acid 2.0 7 Lipophilic monostearic acid glycerin 3.0 8 Macadamia oil 4.0 9 glycerin 4.0 10 Propylene glycol 3.0 11 Butylene glycol 3.0 12 Triethanolamine 1.0 13 Aluminum Magnesium Silicate 0.5 14 Pigment 12.0 15 antiseptic a very small amount 16 Spices a very small amount 17 Purified water Remaining amount

Experimental Example 1: Temperature Storage Stability

The cosmetic preparations of Examples 1 to 6 of Formulation Examples 1 and 2 were prepared using a non-extract extract prepared using ethanol as a solvent in Preparation Example 1, and the temperature storage stability of the cosmetic was evaluated. In order to confirm the temperature storage stability, the stability according to the change of temperature was investigated, and the results are shown in Table 11 below.

Temperature (℃) term Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 -5 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ◎ ◎ ◎ ◎ 4 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ◎ ◎ ◎ ◎ 35 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ○ ◎ ○ ○ 45 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ○ ◎ ○ ○                      ◎: Very good ○: Good x: Separation

From the results in Table 11, it can be seen that the cosmetic containing the non-extract extract of the present invention is very excellent in temperature storage stability.

Experimental Example 2: Clinical Effect of Skin Wrinkle Improvement

The clinical effect of skin wrinkle improvement on cosmetics containing non-extract of the present invention was measured.

This clinical trial divides 20 women over the age of 25 who have started or have already developed wrinkles into 2 groups, the cosmetics of Example 6 in Group A, and the cosmetics of Comparative Example 2 in Group B of the left and right eyes 12 weeks was applied. The degree of improvement of skin wrinkles was measured by classifying the objective evaluation of the expert and the subjective evaluation of the subject into the following four grades. The results are shown in Tables 11 and 12 below.

Skin wrinkle improvement evaluation criteria:

 -: No change

 +: Slightly improved

 ++: improved

 +++: Very Improved

Clinical Effects of Skin Wrinkle Improvement (Objective Evaluation by Skilled Workers) division  Skin wrinkle improvement (after 12 weeks) Example 6 Comparative Example 2 Subject 1 +++ - Subject 2 ++ - Subject 3 ++ + Subject 4 +++ + Subject 5 + - Subject 6 + - Subject 7 ++ - Subject 8 ++ + Subject 9 - - Subject 10 + -

Clinical effect of skin wrinkle improvement (subjective evaluation of subject) division  Skin wrinkle improvement (after 12 weeks) Example 6 Comparative Example 2 Subject 1 +++ ++ Subject 2 ++ - Subject 3 +++ + Subject 4 +++ + Subject 5 + + Subject 6 ++ - Subject 7 + - Subject 8 ++ ++ Subject 9 ++ + Subject 10 + -

As can be seen from Table 12 and Table 13, in the case of Example 6, the objective evaluation of the skilled person and the subjective evaluation of the subject were much higher than those of Comparative Example 2. Therefore, it can be seen that the wrinkle improvement effect of the formulation prepared by adding the non-extract extract of the present invention is very excellent.

As described in detail above, the present invention provides a composition for inhibiting elastase, a composition for promoting collagen synthesis, and a composition for improving skin wrinkles and elasticity, including a visa extract as an active ingredient. The visa extract of the present invention has excellent elastase inhibitory and fibroblast proliferation effects, in particular, the fraction of water or chloroform of the visa extract has excellent elastase inhibitory activity. Cosmetic composition comprising a non-extract extract of the present invention as an active ingredient is excellent in improving the skin wrinkles and skin elasticity, and exhibits excellent stability.

Claims (7)

Elastase inhibition composition comprising a non-extract as an active ingredient. Collagen synthesis promoting composition comprising a non-extract as an active ingredient. Cosmetic composition for improving skin wrinkles and elasticity comprising a non-extract as an active ingredient. The cosmetic composition for improving skin wrinkles and elasticity of claim 3, wherein the non-extract extract improves skin wrinkles and elasticity by inhibiting elastase, promoting collagen synthesis, or promoting fibroblast proliferation. The composition according to any one of claims 1 to 4, wherein the non-extract extract is a water layer fraction. The composition according to any one of claims 1 to 4, wherein the content of the non-extract extract is 0.0001-15.0 wt% based on the total weight of the composition. The composition of claim 3, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation Cosmetic composition for improving skin wrinkles and elasticity, characterized in that having a formulation selected from the group consisting of, wax foundation and spray.