KR20100001167A - Cosmetic composition comprising quercitrin and/or myricitrin as active ingredients - Google Patents

Abstract

PURPOSE: A cosmetic composition containing quercitrin and/or myricitrin is provided to promote protein synthesis and collagen synthesis. CONSTITUTION: A cosmetic composition for anti-aging and wrinkle smoothing contains 0.000001-15.0% of quercitrin and/or myricitrin as an active ingredient. The quercitrin and myricitrin are denoted by chemical formula 1 and 2, respectively. The quercitrin and myricitrin are isolated from Metasequoia glyptostroboides or Korean Rosebay. The cosmetic composition is used in the form of solution, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray foundation.

Description

Cosmetic composition containing quercitrin and / or myrcitrin as an active ingredient {Cosmetic Composition Comprising Quercitrin and / or Myricitrin as Active Ingredients}

The present invention relates to a cosmetic composition for improving skin wrinkles containing quercitrin and / or myrcitrin as an active ingredient.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into internal aging and photo-aging, and research on this has been actively conducted. Free radicals may be activated by UV rays, stress, disease conditions, environmental factors, wounds, and age. If this condition is exacerbated, it destroys antioxidant defenses in vivo and damages cells and tissues. And aging. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins is caused by severe cuts in the connective tissues of collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, and so on, resulting in severe over-inflammatory reaction and skin elasticity. If this worsens, mutations in the DNA can lead to mutations, cancer, and reduced immune function.

Therefore, it protects the cell membrane by eliminating the free radicals caused by metabolic processes of the body, irradiation by UV irradiation and inflammatory reactions, and the already damaged cells should be regenerated by active metabolism to proliferate the cells. You can recover quickly and maintain healthy skin. Aging involves not only free radicals, but also an enzyme called matrix metalloprotease (MMP) .In vivo, the synthesis and degradation of extracellular substrates such as collagen are properly regulated, but their synthesis decreases as aging progresses. Expression of matrix metalloproteinase (MMP), which is an enzyme that degrades, is promoted, thereby decreasing the elasticity of the skin and forming wrinkles. Ultraviolet irradiation also activates these degrading enzymes. Therefore, there is a need for the development of a substance capable of regulating MMP expression that inhibits activation or inhibiting its activity in cells. Until now, most of the raw materials used as materials for cosmetics simply inhibited enzyme activity. Therefore, we tried to regulate the expression and activity of MMP expression induced by intracellular natural aging and photoaging.

On the other hand, a variety of chemicals are used as a cosmetic composition, but recently, cosmetics using natural products have been developed to reduce skin irritation caused by such chemicals. Natural materials have less side effects on the skin, and as the consumer's response to cosmetics using natural materials has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing.

Recently, many cosmetic companies have been making various research and development to overcome the above problems, it has been found that various natural products such as rhododendrons, stalks and potions are effective in preventing skin aging. However, most of these components have a problem such that the relative effect of the stability of the cosmetic, skin irritation and concentration is weakened when used in a high concentration as an extract mixed with various components.

The present inventors earnestly researched to improve the skin aging phenomenon by promoting the synthesis of skin proteins damaged by ultraviolet light, etc. As a result, the composition containing quercitrin and myricitrin enhances the protein synthesis of fibroblasts. The present invention was completed by discovering that the effect of enhancing the collagen synthesis of the dermis is excellent, and the effect of inhibiting the production of MMP-1 and improving skin wrinkles.

Accordingly, an object of the present invention is to provide a cosmetic composition for preventing skin aging containing as an active ingredient containing any one or more of quercitrin and myrcitrin (myricitrin).

Another object of the present invention to provide a cosmetic composition for improving skin wrinkles containing any one or more of quercitrin and myrcitrin (myricitrin) as an active ingredient.

Another object of the present invention is to provide a cosmetic method comprising applying to the human skin a cosmetic composition containing at least one of quercitrin and myrcitrin (myricitrin).

Other objects and advantages of the present invention will become apparent from the following examples and claims.

The present invention provides a cosmetic composition comprising a quercitrin and myrcitrin as an active ingredient. More specifically, the present invention relates to a cosmetic composition for preventing skin aging and improving skin wrinkles, which contains any one or more of quercitrin and myrcitrin as active ingredients. The composition containing quercitrin and myrcitrin of the present invention is excellent in improving protein synthesis, enhancing collagen synthesis, inhibiting MMP-1 production, and improving skin wrinkles.

The present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles, including any one or more of quercitrin and myrcitrin (myricitrin) as an active ingredient.

'Quercitrin' included as an active ingredient of the present invention has a molecular formula of C ₂₁ H ₂₀ O ₁₁ , 3,3 ', 4', 5,7-pentahydroxyflavone-3- O- aL-namno It is a compound having a molecular weight of 448 having a structure represented by the following Chemical Formula 1 represented by pyranoside (3,3 ', 4', 5,7-pentahydroxyflavone-3- O- aL-rhamnopyranoside).

Another active ingredient 'myricitrin' of the present invention is a compound having a structure of Formula 2, having a molecular formula of C ₂₁ H ₂₀ O ₁₂ and a molecular weight of 464.

Quarcitrin and myricitrin used in the present invention can be separated from natural sources such as metasequoia, bark bark, rhododendron, and chemically synthesized.

On the other hand, the quercitrin and myrcitrin used in the present invention was isolated from the metasequoia (Metasequoia glyptostroboides), a conifer plant of the old tree, and the metasequois are distributed in Sichuan, Hubei, and Korea. The average size of an individual reaches 35m in height and 2m in diameter, but it grows larger and thicker depending on the environment and lifespan. The branches spread to the side and the bark peels off brown. Twigs are green and face each other. The leaves face each other in a row shape, 10-23cm long, and 1.5-2mm butterfly. It is arranged in a feather and its end is suddenly pointed. In autumn, reddish brown foliage falls and falls with small branches. It is mainly used as a roadside or wind dimension, and is also used as a soundproofing device or a packing material in a room.

According to a preferred embodiment of the present invention, quercitrin and myricitrin are each 0.000001-15.0% by weight, preferably 0.0001-10.0% by weight, based on the total composition.

At this time, if each content of quercitrin and myrcitrin (myricitrin) contained in the cosmetic composition is less than the minimum content, the expected effect is unlikely to occur, and if the content exceeds the maximum content, the effect does not increase any more and is applied to the skin. It is likely to cause irritation and has a big impact on the stabilization of the formulation.

According to the present invention, quercitrin and myrcitrin have a protein synthesis promoting effect (Experimental Example 1), dermal layer collagen synthesis enhancing effect (Experimental Example 2), MMP-1 production inhibitory effect (Experimental Example 3) outstanding. In addition, the cosmetic composition containing the mixture is excellent in the skin wrinkle improvement effect (Experimental Example 4).

Therefore, the cosmetic composition comprising a mixture of quercitrin and myrcitrin with various functions as an active ingredient has an excellent effect on promoting protein synthesis, enhancing dermal collagen synthesis, inhibiting MMP-1 production and improving skin wrinkles. Has

In addition, mixtures of derivatives obtained by addition or substitution reaction of substituents commonly practiced in the art with respect to the quercitrin and myricitrin compounds of Formulas 1 and 2 are also included in the scope of the present invention. This is apparent to those skilled in the art in view of the state of the art.

The composition of the present invention can achieve its effect by topical application or spraying on the skin. Therefore, according to a preferred embodiment of the present invention, the composition of the present invention may be prepared in the form of cosmetic compositions such as creams, lotions and softening water or pharmaceutical compositions such as sprays and ointments.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid as carrier components Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention provides a cosmetic method comprising applying a cosmetic composition containing any one or more of the quercitrin and myrcitrin (myricitrin) of the present invention to the human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is cleansing Creams, cleansing lotions, cleansing water, and cleansing gels.

When the cosmetic method including any one or more of quercitrin and myrcitrin of the present invention is applied to human skin, an excellent wrinkle improvement effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1. Isolation and Identification of Quarcitrin and Myricitrin

Quercitrin and myrcitrin used in the present invention were isolated and identified by the following method.

About 1.1 kg of dry metasequoia leaves were immersed in 5 L of 70% ethanol for 5 days, filtered and the filtrate was dried under reduced pressure. This was repeated twice to obtain about 100 g of concentrate (meta sequoia crude extract). This was dissolved in distilled water, and then fractionated with n-hex, EA (ethyl acetate), n-BuOH, and water layers to load the ethyl acetate layer into a column tube filled with silica 230-400 mesh, and ethyl acetate as a developing solvent. 20 mL fractions were added with methanol flowing in a 30: 1 to 0: 1 gradient. Fractions up to Rf = 0.35-4.0 were concentrated by qualitative analysis with a thin membrane chromatograph, and the fractions were fractionated with MeOH on a column filled with Sepadex20.

By the above fractions, MG-1 and MG-2 having a particularly effective effect were finally obtained, and the structure was analyzed using NMR (see results below). As a result, MG-1 and MG-2 were identified as quercitrin and myrcitrin.

Quercitrin 1H NMR (CD3OD, 400 MHz): 7.33 (1H, d, J = 2.0 Hz, H-2 '), 7.30 (1H, dd, J = 8.2, 2.0 Hz, H-6'), 6.95 (1H , d, J = 8.2 Hz, H-5 '), 6.36 (1H, d, J = 2.0 Hz, H-8), 6.19 (1H, d, J = 2.0 Hz, H-6), 5.35 (1H, d, H-1 ''), 4.23 (1H, m, H-5``), 3.75 (1H, dd, H-2 ''), 3.41 (1H, m, H-3 ''), 3.35 ( 1H, m, H-4 ''), 0.94 (3H, d, J = 6.0 Hz, H-6``). 13C NMR (CD3OD, 100 MHz): 157.3 (C-2), 135.0 (C-3 ), 178.4 (C-4), 162.0 (C-5), 98.6 (C-6), 164.6 (C-7), 93.5 (C-8), 158.1 (C-9), 104.7 (C-10) , 121.8 (C-1 '), 115.2 (C-2'), 145.2 (C-3 '), 148.6 (C-4'), 115.8 (C-5 '), 121.7 (C-6'), 102.3 (C-1``), 70.8 (C-2 ''), 70.9 (C-3``), 72.1 (C-4 ''), 70.7 (C-5 ''), 16.5 (C-6 '').

Mycitrinin 1H NMR (CD3OD, 400 MHz): 6.95 (2H, s, H-2 ', 6'), 6.35 (1H, d, J = 2.0 Hz, H-8), 6.19 (1H, d, J = 2.0 Hz, H-6), 5.31 (1H, d, H-1``), 4.22 (1H, m, H-5``), 3.78 (1H, dd, H-2 ''), 3.50 (1H , m, H-3``), 3.36 (1H, m, H-4``), 0.96 (3H, d, J = 6.4 Hz, H-6``). 13C NMR (CD3OD, 100 MHz): 157.3 (C-2), 135.1 (C-3), 178.5 (C-4), 162.0 (C-5), 98.6 (C-6), 164.6 (C-7), 93.5 (C-8), 158.2 (C-9), 104.7 (C-10), 120.7 (C-1 '), 108.4 (C-2'), 145.6 (C-3 '), 136.7 (C-4'), 145.6 (C-5 '), 108.4 (C-6'), 102.4 (C-1``), 70.8 (C-2 ''), 70.9 (C-3 ''), 72.1 (C-4 ''), 70.7 (C -5 ''), 16.5 (C-6 '').

Experimental Example 1. Experiment of protein synthesis promoting effect of quatcitrin and myricitrin

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 1 x 10 6 cells in each well of a 48-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the medium was replaced with serum-free DMEM medium (experimental group) prepared so that the quercitrin and myrcitrin obtained in Preparation Example 1 were 5, 10, 20, 50 and 100 ppm, respectively, and further incubated for 24 hours. It was. After incubation, the supernatant of each well was collected and the amount of newly synthesized protein was measured using a protein assay kit (Protein assay kit, Biorad, USA). The results are shown in Table 1 below. At this time, the control group was incubated with a serum-free medium replaced with purified water instead of the active ingredient of the present invention, and as a comparative experiment group, a serum-free medium containing metasequoia crude extract obtained by extracting metasequoia from 70% ethanol and then filtering and concentrating. Alternate cultures.

Protein synthesis promoting effect (protein production amount (㎍ / ㎖)) Concentration (μg / ml) Quacitrine Mycitrinin Metasequoia Crude Extract 5 94.2 87.6 68.3 10 99.3 95.3 77.3 50 108.3 1003.7 84.2 100 115.2 109.8 88.6 Control 50.3

As can be seen from the results of Table 1, the amount of protein produced in the experimental group to which the quasi- citrin and mycitrin was added and the comparative group to which metasequoia crude extract was added were significantly higher than the amount of protein produced in the control group. In particular, the comparative experimental group to which the metasequoia crude extract, which is present in a mixture of various mixtures, confirmed higher protein synthesis effects than the experimental group to which each added quercitrin or myricitrin.

Experimental Example 2. Effects of Quercitrin and Myrcitrin on the Dermal Layer Collagen Synthesis

Human normal fibroblasts (Korea Cell Line Bank) were seeded in each well of a 48-well microplate to be 1 × 10 6 cells and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the serum-free DSA medium (experimental group) and metasequoia crude extract prepared so that the final concentrations of the quercitrin and myrcitrin obtained in Preparation Example 1 were 5, 10, 20, 50, and 100 ppm, respectively. -DMEM medium (comparative group) was replaced and further incubated for 24 hours. The supernatant of each well was collected and the amount of C-peptide (PICP) of newly synthesized procollagen type I was measured using a kit (PICP collagen assay kit, Takara, Japan). PICP amount was converted to ng / ml, and the collagen production increase rate was calculated using Equation 1 below. The results are shown in Table 2 below. In this case, serum-free DMEM medium containing TGF-β (10 ng / ml, Roche, United States) was used as a positive control, and serum-free DMEM medium containing none of the above components was used as a negative control.

Concentration (μg / ml) Quacitrine Mycitrinin Metasequoia Crude Extract Negative Control 130.4 5 247.5 198.6 210.3 10 335.4 295.3 250.5 50 408.3 401.5 280.6 100 415.2 409.8 350.8 TGF-β 10ng / ml 410.3

As shown in Table 2, the amount of collagen produced in the experimental group to which the quasi- citrin or mycitrin was added and the comparative test to which the metasequoia crude extract was added were significantly higher than the amount of collagen produced in the negative control group containing nothing. , 100 ㎍ / mL TGF-b was used as a positive control. In particular, the comparative experimental group to which the metasequoia crude extract, which is a mixture of a variety of different mixtures was confirmed, the effect of protein synthesis higher than the experimental group to add a quercitrin or myricitrin.

Experimental Example 3. Inhibitory effect of quatcitrin and mycitrin on MMP-1 production

Fibroblasts (Korean Cell Line Bank, Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 × 10 ⁶ cells per well, and treated with DMEM medium (Sigma, USA) and 37 ° C. After culturing for 24 hours at 48 hours in serum-free DMEM medium and the control group was added serum-free DMEM medium and quasi-citrinin and metasequoia crude extract obtained in the final concentration of 1% and control group 48 Further incubation for hours. After incubation, the supernatants of each well were collected and the amount of ng / ml newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA), and MMP-1 production was performed according to Equation 1 below. Inhibition rate was calculated and the results are shown in Table 3. At this time, TGF-β (10 ng / ml, Roche, United States) was used as a positive control of MMP-1 production inhibition rate.

MMP-1 Production Inhibition Rate (%) Test substance % Inhibition of MMP-1 production Quacitrine 83.3 Mycitrinin 83.2 Metasequoia Crude Extract 63.5 TGF-b 79.5

Hereinafter, in the formulation example, but was used quatrecitrin and myricitrin obtained from Preparation Example 1 of the present invention, but is not limited thereto.

Formulation Example 1. Softening Longevity

Formulation examples of the flexible longevity was prepared as follows according to a conventional method.

ingredient Content (unit: weight%) Content (unit: weight%) Quatcitrin Myriline Glycerin 1.3-Butylene Glycol PEG 1500 Allantoin DL-Panthenol E.D.T.A-2NA Benzophenone-9 Sodium Hyaluronate Ethanol Octicdodeceth-16 Polysorbate 20 Preservatives, Flavors, Colors Distilled Water            1.0-5.0 3.0 1.0 0.1 0.3 0.02 0.04 5.0 10.0 0.2 0.2 Micro balance             -1.0 5.0 3.0 1.0 0.1 0.3 0.02 0.04 5.0 10.0 0.2 0.2 Micro balance Sum 100 100

Formulation Example 2. Converging Cosmetic Water

Formulation examples of convergent longevity were prepared as follows according to a conventional method.

ingredient Content (unit: weight%) Content (unit: weight%) Quatricitrimyriline glycerin 1.3-butylene glycol allantoin DL-panthenol E.D.T.-2-2 benzophenone-9 sodium hyaluronate ethanol polysorbate 20 witch hazel extract citric acid preservative, fragrance, pigment distilled water           1.0-2.0 2.0 0.2 0.2 0.02 0.04 3.0 15.0 0.3 2.0            -1.0 2.0 2.0 0.2 0.2 0.02 0.04 3.0 15.0 0.3 2.0      Sum 100 100

Formulation Example 3. Nutrients

Formulation examples of nutrient cosmetics was prepared as follows in a conventional manner.

ingredient Content (unit: weight%) Content (unit: weight%) Quatricitrimyriline glyceryl stearate SE stearyl alcohol lanolin polysorbate 60 sorbitan stearate hardened vegetable oil mineral oil squalane trioctanoin dimethicone acetate tocopherol carboxyvinyl polymer glycerin 1.3-butylene glycol sodium hyaluronate tri ethanolamine preservative, Incense, Pigment Distilled Water            1.5-1.5 1.5 1.5 1.3 0.5 1.0 5.0 3.0 2.0 0.8 0.5 0.12 5.0 3.0 5.0 0.12            1.5 1.5 1.5 1.5 1.3 0.5 1.0 5.0 3.0 2.0 0.8 0.5 0.12 5.0 3.0 5.0 0.12 Sum 100 100

Formulation Example 4. Nutrition Cream

Formulation of nutrition cream was prepared as follows according to a conventional method.

ingredient Content (unit: weight%) Content (unit: weight%) Quatricitrimyriline lipophilic monostearate glycerine cetearyl alcohol stearic acid beeswax polysorbate 60 sorbitan stearate hardened vegetable oil squalane mineral oil trioctanoin dimethicone sodium magnesium silicate glycerin betaine triethanolamine sodium hyaluronate preservative Pigmented distilled water            1.0-2.0 2.2 1.5 1.0 1.5 0.6 1.0 3.0 5.0 5.0 1.0 0.1 5.0 3.0 1.0 4.0            -1.0 2.0 2.2 1.5 1.0 1.5 0.6 1.0 3.0 5.0 5.0 1.0 0.1 5.0 3.0 1.0 4.0 Sum 100 100

Formulation Example 5. Massage Cream

Formulation example of the massage cream was prepared as follows according to a conventional method.

ingredient Content (unit: weight%) Content (unit: weight%) Quatricitrimyriline lipophilic monosporic acid glycerin stearyl alcohol stearic acid polysorbate 60 sorbitan stearate isostearyl isosterate squalane mineral oil dimethicone hydroxyethyl cellulose glycerin triethanolamine preservative, fragrance, pigment distilled water        2.0-1.5 1.5 1.0 1.5 0.6 5.0 5.0 35.0 0.5 0.12 6.0 0.7 Microbalance         -2.0 1.5 1.5 1.0 1.5 0.6 5.0 5.0 35.0 0.5 0.12 6.0 0.7 Micro balance Sum 100 100

Formulation Example 6. Essence

Formulation examples of the essence was prepared as follows in a conventional manner.

ingredient Content (unit: weight%) Content (unit: weight%) Quatcitrin Myriline Glycerin Betaine Fiji 1500 Allantoin DL-Pantenol E.D.T .-- 2Na Benzophenone-9 hydroxyethyl cellulose sodium hyaluronate carboxyvinylpolymer triethanolamine octyldodecanol octyldodeces -16 Ethanol preservative, fragrance, pigment distilled water            2.0 2.0 10.0 5.0 2.0 0.1 0.3 0.02 0.04 0.1 8.0 0.2 0.18 0.3 0.4 6.0            2.0 2.0 10.0 5.0 2.0 0.1 0.3 0.02 0.04 0.1 8.0 0.2 0.18 0.3 0.4 6.0 Sum 100 100

Formulation Example 7 Pack

Formulation examples of the pack were prepared as follows in a conventional manner.

ingredient Content (unit: weight%) Content (unit: weight%) Quatcitrin Myriline Polyvinyl Alcohol Cellulose Gum Glycerin Fiji 1500 Cyclostrin DL-Panthenol Allantoin Glycyric Acid Monoammonium Nicotinamide Ethanol Fiji 40 Cured Castor Oil Flavor, Pigment, Preservative Distilled Water           1.0-15.0 0.15 3.0 2.0 0.15 0.4 0.1 0.3 0.5 6.0 0.3 Micro balance           -1.0 15.0 0.15 3.0 2.0 0.15 0.4 0.1 0.3 0.5 6.0 0.3 Micro balance Sum 100 100

Experimental Example 4. Improvement of skin wrinkles and elasticity

Clinical trials were carried out for the skin wrinkle improvement effect of the cream cosmetics containing the quercitrin and myrcitrin of the present invention. Clinical trials were evaluated through the actual use test of each cosmetic.

The experiment was used together on one cheek of each subject using the nutrient cream of Formulation Example 4 obtained from Preparation Example 1 or the nutrient cream of Formulation Example 4 containing the component replaced with purified water in Formulation Example 4 as a comparative example. It was made. Among 40 30-40 year old women, women who had a lot of external activity were randomly divided into two groups, and the creams of Formulation Example 4 and Comparative Example were washed two times every morning and then the appropriate amount was corrected continuously for two months centering on the eyes. The wrinkle improvement effect of each subject was evaluated through visual observation by an experienced doctor. The experimental results are shown in Table 11 below.

Wrinkle improvement effect (visual evaluation) of the cosmetics of the present invention Wrinkle improvement Effectiveness% Great slightly none Quatcirine Cream 10 11 4 62.2 Myristicin-containing cream 9 14 2 63.3 Comparative cream 3 4 23 44.4

   As can be seen from the results of Table 11, the cream of the formulation example 4 containing the quoitrine or myrithinlin showed a higher anti-wrinkle effect than the cream of the comparative example without any. Moreover, the skin irritation could not be observed in the skin of the subjects who apply the cosmetic composition of the present invention to the skin.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

    A cosmetic composition for preventing skin aging containing any one or more of quercitrin and myrcitrin as an active ingredient.     Cosmetic composition for improving skin wrinkles containing any one or more of quoitrine and myrcitrin as an active ingredient.     The cosmetic composition according to claim 1 or 2, wherein the content of the quercitrin and myrcitrin is contained in 0.000001-15.0% of the total weight of the cosmetic composition.     3. The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation And a formulation selected from the group consisting of sprays. The cosmetic method according to any one of claims 1 to 4, wherein the cosmetic composition is applied to human skin.