KR100537914B1 - Cosmetic Composition Comprising Calyx Kaki as Active Ingredient for Improving Skin Wrinkle and Elasticity - Google Patents

Abstract

The present invention provides a composition for inhibiting elastase and a composition for improving skin wrinkles and elasticity comprising the extract as an active ingredient. The extract of the present invention has excellent elastase inhibition and fibroblast proliferation effect, in particular, the fraction of water or chloroform of the extract has excellent elastase inhibitory activity. Cosmetic composition containing the extract of the present invention as an active ingredient is excellent in improving the skin wrinkles and skin elasticity, and shows excellent stability.

Description

Cosmetic composition for improving skin wrinkles and elasticity comprising the extract as a active ingredient {Cosmetic Composition Comprising Calyx Kaki as Active Ingredient for Improving Skin Wrinkle and Elasticity}

 The present invention relates to a composition comprising a teat extract, and more particularly, the present invention relates to a composition for inhibiting elastase and a composition for improving skin wrinkles and elasticity comprising the extract as an active ingredient.

Skin is an organ that covers the body's surface. The skin is largely divided into epidermis and dermis. The epidermis is the outermost of the skin and acts as a protective barrier for internal organs. The epidermis is composed of a stratum corneum, a clear layer, a granule layer, a play layer and a base layer. The dermis serves to nourish the epidermis and form the skin's framework within the skin tissue. The dermis is composed of a papillary layer and a deep reticular layer, which is a place where skin cell proliferation is induced by fibroblast activity. The reticular layer plays a role in maintaining the elasticity of the entire skin, and collagen (collagen) and elastin (elastic fiber) are meshed (Braverman, J. Invest. Dermatol. , 78, 434-443 (1982). )).

Elastic fibers are elastic fibers included in connective tissue and are much more stretchable than collagen fibers included in connective tissue. Elastic fibers provide elasticity to the skin so that the deformed skin can be restored to its original shape by the force applied to the skin.

Elastin is the main component of the elastic fiber is composed of elastin (90%) of the amorphous insoluble protein 90% of the elastic fiber. Elastase is an enzyme that degrades elastin, and the breakdown of elastin by elastase decreases the elasticity of the skin and accelerates the production of wrinkles (Dermatology pp 18, Korean Dermatology Textbook). Committee compilation, Ryeomungak)

Therefore, a method of inhibiting the degradation of elastin by inhibiting the activity of elastase as a method for improving skin wrinkles and elasticity has been reported (Kim In-young et al . , Kor. J. Pharmacogn 33 (4): 364-371 (2002)).

Wrinkle improvement materials, such as retinoids used in the prior art has a low stability, there is a limit to use in cosmetics, irritation and redness occurs during application of the skin, there is also a safety problem, the amount of use is limited.

Therefore, there is a need for development of a new material having excellent stability and safety as well as an effect of improving wrinkles and elasticity.

Throughout this specification, a number of articles are referenced and their citations are indicated. The disclosures of cited articles are incorporated herein by reference in their entirety to more clearly describe the level of the technical field to which the present invention pertains and the content of the present invention.

The inventors of the present invention conducted a thorough research on herbal medicines to find a substance having high stability and excellent skin wrinkle and elasticity improvement. As a result, the extract has excellent elastase inhibitory activity and fibroblast proliferation effect, and stability was also excellent. The present invention was completed.

Therefore, it is an object of the present invention to provide a composition for inhibiting elastase, which comprises a extract of persimmon as an active ingredient.

Another object of the present invention to provide a cosmetic composition for improving skin wrinkles and elasticity comprising the extract extract as an active ingredient.

According to one aspect of the present invention, the present invention provides a composition for inhibiting elastase comprising the extract of persimmon as an active ingredient.

According to another aspect of the present invention, the present invention provides a cosmetic composition for improving skin wrinkles and elasticity comprising the extract as an active ingredient.

The present invention is characterized in that it comprises an extract of the persimmon which is calyx of persimmon fruit as an active ingredient.

Is touching the faucet (body) is the Latin name Carl Riggs Khaki (Kaki Calyx) as the fruit buds of deciduous tall tree which Sophie de los Khaki (Disopyros kaki L.) Of ebenaceae. It is effective in relieving vomiting and coughing, and has been used as a medicinal herb in herbal medicine. The main components of the teat include succinic acid, quercetin, kaempferol, neoodiospyrin, oleanolin, ursolic, betulin and doucosterol. (daucosterol) and others are known (International Collation of Traditional and Folk Medicine, pp 125-126, World Scientific (1997)).

The teat extract used in the present invention may be obtained through various methods known in the art, and preferably comprises (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (eg, methanol, Ethanol, propanol, butanol and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol. The extraction solvent is more preferably alcohol, most preferably ethanol and methanol. The nipple as the material for extraction can be dried or used as it is, and preferably used by drying. As a drying method, natural drying or a hot air drying method can be used.

Referring to the following specific preparation of the present invention, a method for preparing a teat extract is described as follows: The teat is washed with purified water, dried and then ground. The milled teat fine powder is placed in methanol or ethanol and leached for several days at room temperature. The leachate was recovered by filtering with Whatman filter paper, and re-leached with methanol or ethanol. The releach process is repeated several times and the recovered leachate is concentrated in a vacuum concentrator.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also extract extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the extract of the teat of the present invention.

The teat extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

According to a preferred embodiment of the present invention, the extract for use in the present invention, after the extraction using the above-mentioned extraction solvent, for further fractionation of the active ingredient, water and organic solvents (eg, hexane, ethyl acetate) And chloroform). Thus, the extract of the present invention is more preferably a water layer fraction and a chloroform layer fraction, and most preferably a chloroform layer fraction.

The present inventors determined the elastase inhibitory effect of the fraction of each solvent by fractionation of the persimmon extract with various solvents, and as a result, it was confirmed that the water layer and the chloroform layer fractions had an excellent elastase inhibitory effect, in particular, chloroform It was confirmed that the layer fraction had the best effect.

From the above fact, it can be seen that the substance having high solubility in chloroform is particularly excellent in elastase inhibitory effect in the extract of the present invention. In addition, fractionation of the extract by the solvent allows removal of unnecessary substances and the use of only essential ingredients.

Referring to the following specific preparation of the present invention, the solvent-specific fractions of the extract of the teat will be described as follows: The teat extract obtained using the extraction solvent was dispersed in purified water and shaken by adding hexane. After the hexane layer and the water layer were sufficiently separated, an upper hexane layer was taken. Hexane was added to the lower layer (water layer) to extract several more times. After all the recovered upper layers were combined, the solvent was removed using a vacuum distillation apparatus. The lower layer (water layer) is reextracted and concentrated in the same manner as above using chloroform and ethyl acetate instead of hexane. Finally, the water layer is distilled under reduced pressure to obtain a water layer fraction.

The teat extract of the present invention has an excellent effect of inhibiting elastase activity. The reticular layer, which plays a role of maintaining the elasticity of the entire skin, is composed of collagen (collagen fiber) and elastin (elastic fiber) in a net shape. The elasticity of the skin is greatly influenced by elastic fibers, which are mainly composed of elastin, and when the elastin of the elastic fibers is decomposed by elastin degrading enzymes, the skin loses elasticity and accelerates wrinkle formation. The teat extract of the present invention inhibits the degradation of elastin by elastinase by inhibiting the elastase activity so that the elastic fiber can perform its function smoothly, and as a result, the elasticity of the skin is maintained and improved and further wrinkles The production of is suppressed and the effect of improving wrinkles is obtained.

On the other hand, the teat extract of the present invention promotes the proliferation of fibroblasts constituting the fibrous connective tissue of the dermis. The proliferative effect of the fibroblasts by the extract of the present invention enables the supply of healthy young cells to the fibrous connective tissues, resulting in the effect of strengthening the fibrous connective tissue of the skin together with the elastase activity of the extract .

According to a preferred embodiment of the present invention, the content of the teat extract is 0.0001 to 15% by weight, preferably 0.001 to 10% by weight and more preferably 0.01 to 5% by weight based on the total composition. If the amount of the extract is less than 0.0001% by weight, there is a problem that the effect does not appear, and when the amount exceeds 15% by weight, there is no significant difference in the effect.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to the extract of persimmon as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention comprising the extract as an active ingredient is not only inhibits the activity of the elastase, but also promotes fibroblast proliferation to improve skin wrinkles and elasticity. In addition, the cosmetic composition containing the extract of the present invention exhibits excellent temperature stability.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Example

Preparation Example 1 Preparation of Tap Extract

The pacifier was washed with purified water, dried and then ground. 200 g of the ground teat fine powder was added to 1 L of methanol or ethanol and leached at room temperature for 2 days. The leachate was recovered by filtering the filter using Whatman No. 2, and then re-leached with 1 L of methanol or ethanol. The releach process was repeated twice. The recovered leachate was concentrated with a vacuum concentrator, and 10 g of dry extract was obtained.

Preparation Example 2 Preparation of Fractions by Solvent

The methanol extract of the teat obtained in Preparation Example 1 was dispersed in 1 L of purified water and shaken by adding 1 L of hexane. After the hexane layer and the water layer were sufficiently separated, an upper hexane layer was taken. Hexane was added to the lower layer (water layer) to extract twice more. The combined upper layers were combined and the solvent was removed using a vacuum distillation apparatus to obtain 670 mg of a hexane layer fraction. Using chloroform and ethyl acetate instead of hexane, the lower layer (water layer) was reextracted and concentrated in the same manner as above to obtain 4.4 g of chloroform fraction and 1.1 g of ethyl acetate layer. Finally, the water layer was distilled under reduced pressure to obtain 3.6 g of a water layer fraction.

Experimental Example 1: Elastase inhibition assay

60 μl of a solution prepared for each concentration of the teat extract of Preparation Example 1 (solvent: 100 mM Tris HCl pH 8.0) and 100 μl of a 0.6 units / ml elastase enzyme (Sigma, United States) solution were reacted at 37 ° C. for 30 minutes. . Then, 40 μl of a 4 mM sucAla3-PNA solution (Sigma, USA) was added and reacted at 37 ° C. for 30 minutes, and then absorbance was measured at 405 nm using a spectrophotometer. The experimental results are shown in Table 1 below. Elastase inhibitory activity was calculated according to the following equation.

% Inhibition = {1- [comparative absorbance (OD ₄₀₅ ) / control absorbance (OD ₄₀₅ )]} × 100

Persimmon Extract (mg / ml) Elastase Inhibitory Activity (%) (Methanol Extraction) Elastase inhibitory activity (%) (ethanol extraction) 0.03 43.9 38.3 0.3 81.8 84.9 0.45 86.4 86.0

As shown in Table 1, the extract was inhibited the activity of elastase in a concentration-dependent manner, the inhibitory effect was excellent.

Experimental Example 2: Elastase inhibitory effect of the solvent-specific fractions of the extract

The elastase inhibitory effect of the solvent-specific fractions of hexane, chloroform, ethyl acetate and water obtained in Preparation Example 2 was measured by the method described in Experimental Example 1.

Fraction by solvent (0.45mg / ml) Elastase Inhibitory Activity (%) Hexane layer 24.8 Chloroform layer 90 Ethyl acetate layer 58.8 Water layer 78.6

As can be seen in Table 2, the elastase inhibitory activity was observed in all solvent layers, but the water layer and the chloroform layer showed superior elastase inhibitory activity compared to the other layers, and the chloroform layer showed the highest inhibitory activity. Therefore, it can be expected that a substance having high elastase inhibitory activity exists in the chloroform layer.

Experimental Example 3: Fibroblast Proliferation Effect Experiment

3-1: Primary Cell Culture of Rat Fibroblasts

SD rats (4 weeks old) purchased from BIO GEOMENIX were anesthetized, their backs were removed, and tissues were extracted. After removing the epidermis and the dermis from the extracted tissue, fibroblasts were isolated from the dermis to confirm the morphology. The identified fibroblasts were stabilized at 37 ° C. for 3-5 days with 5% CO ₂ concentration in the incubator in a 25 cm 2 tissue culture flask by adding 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin to DMEM medium. It was. After stabilization, passages were treated with trypsin-EDTA solution and initial passage cells (passage 3) were used.

3-2: Fibroblast Proliferation Rate Measurement

Normal rat fibroblasts 4 × 10 ⁴ in logarithmic cells in each well of a 96-well plate Cells / well were seeded and incubated in DMEM medium for 24 hours. Methanol of Preparation Example 1 was exchanged with DMEM medium containing 5% serum treated so that the final concentration of the extract of the teat obtained using 0.01, 0.1 and 1 mg / ml, respectively, and incubated for 72 hours. 50 μl (5 mg / ml) of the MTT solution was added and centrifuged after 4 hours to remove the supernatant. After 100 μl of DMSO was added, the absorbance was measured with an ELISA reader at 570 nm. Cell growth rate (%) was calculated according to the following equation (2).

Cell growth rate (%) = (absorbance of extract treated group-absorbance of control group) / absorbance of control group × 100

Persimmon Extract (mg / ml) Cell proliferation effect (%) 0.01 103.2 0.1 96.5 One 84.1

As shown in Table 3, the extract was found to have a cell proliferation effect of normal rat fibroblasts. When the concentration of the extract was 0.01 mg / ㎖, the highest proliferation effect was obtained, and as the concentration was increased, the proliferation effect was decreased. However, because it showed an excellent overall effect, the extract was found to have an excellent cell proliferation effect.

Hereinafter, examples of the formulation of the present invention include a flexible lotion, a nutrient lotion and a nourishing cream, but the formulation of the cosmetic including the composition of the present invention is not limited thereto.

Formulation example

Formulation Example 1

Formulation examples of the skin lotion (skin) in the cosmetic composition containing the extract is shown in Table 4.

number Raw material name Example 1 (% by weight) Example 2 (% by weight) Example 3 (% by weight) Comparative Example 1 (% by weight) One  Faucet extract 0.01 1.00 5.00 - 2  glycerin 2.00 2.00 2.00 2.00 3  Butylene Glycol 3.00 3.00 3.00 3.00 4  Dipropylene glycol 2.00 2.00 2.00 2.00 5  EDTA-2Na 0.01 0.01 0.01 0.01 6  Pigment a very small amount a very small amount a very small amount a very small amount 7  Ethanol (95%) 5.00 5.00 5.00 5.00 8  antiseptic a very small amount a very small amount a very small amount a very small amount 9  Octyldodeceth-16 0.10 0.10 0.10 0.10 10  incense a very small amount a very small amount a very small amount a very small amount 11  Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Formulation Example 2

Formulation examples of the nutrition lotion in the cosmetic composition containing the extract is shown in Table 5.

number Raw material name Example 4 (wt%) Example 5 (% by weight) Example 6 (% by weight) Comparative Example 2 (% by weight) One  Faucet extract 0.01 1.00 5.00 - 2  Lipophilic glyceryl stearate 1.50 1.50 1.50 1.50 3  Stearic acid 0.30 0.30 0.30 0.30 4  Stearyl alcohol 0.50 0.50 0.50 0.50 5  Beeswax 0.40 0.40 0.40 0.40 6  Polysorbate 60 1.20 1.20 1.20 1.20 7  Sorbitan sesquioleate 0.30 0.30 0.30 0.30 8  antiseptic a very small amount a very small amount a very small amount a very small amount 9  Squalane 3.00 3.00 3.00 3.00 10  Cetylethylhexanoate 5.00 5.00 5.00 5.00 11  Cyclomethicone 4.00 4.00 4.00 4.00 12  glycerin 3.00 3.00 3.00 3.00 13  Butylene Glycol 3.00 3.00 3.00 3.00 14  EDTA-2Na 0.03 0.03 0.03 0.03 15  Triethanolamine 0.15 0.15 0.15 0.15 16  Carbomer 0.12 0.12 0.12 0.12 17  Pigment a very small amount a very small amount a very small amount a very small amount 18  incense a very small amount a very small amount a very small amount a very small amount 19  Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Formulation Example 3

Table 6 shows an example of the formulation of nutrition cream in the cosmetic composition containing the extract.

Number Raw material weight% One   Faucet extract 10.0 2 Beeswax 8.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 20.0 6 Sorbitan stearate 1.0 7 Lipophilic Monostearate Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 6.0 11 Triethanolamine 0.2 12 antiseptic a very small amount 13 Pigment a very small amount 14 Spices a very small amount 15 Purified water Remaining amount

Formulation Example 4

Formulation example of the massage cream in the cosmetic composition containing the extract is shown in Table 7.

Number Raw material weight% One  Faucet extract 10.0 2 Beeswax 10.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.8 5 Liquid paraffin 40.0 6 Squalane 5.0 7 Lipophilic Monostearic Acid Glycerin 1.0 8 Carboxy Vinyl Polymer 0.2 9 Butylene glycol 6.0 10 glycerin 6.0 11 Triethanolamine 0.2 12 antiseptic a very small amount 13 Pigment a very small amount 14 Spices a very small amount 15 Purified water Remaining amount

Formulation Example 5

Formulation examples of the nutritional essence in the cosmetic composition containing the extract is shown in Table 8.

Number Raw material weight% One   Faucet extract 5.0 2 Cytostolol 13.0 3 Polyglyceryl 2-oleate 0.2 4 Ceramide 0.1 5 Ceteares-4 5.0 6 cholesterol 0.3 7 Dicetylphosphate a very small amount 8 Concentrated glycerin 1.0 9 Macadamia oil 0.2 10 Carboxy Vinyl Polymer a very small amount 11 Xanthan Gum a very small amount 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount

Formulation Example 6

Formulation examples of the emulsion-based foundation in the cosmetic composition containing the extract is shown in Table 9.

Number Raw material weight% One   Faucet extract 10.0 2 Beeswax 2.0 3 Pyromethicone 2.0 4 Liquid paraffin 5.0 5 Squalane 5.0 6 Stearic acid 2.0 7 Lipophilic monostearic acid glycerin 3.0 8 Macadamia oil 4.0 9 glycerin 4.0 10 Propylene glycol 3.0 11 Butylene glycol 3.0 12 Triethanolamine 1.0 13 Aluminum Magnesium Silicate 0.5 14 Pigment 12.0 15 antiseptic a very small amount 16 Spices a very small amount 17 Purified water Remaining amount

Experimental Example 1: Temperature Storage Stability

The cosmetic preparations of Examples 1 to 6 of Formulation Examples 1 and 2 were prepared using the extract of the pacifier produced by using ethanol as a solvent in Preparation Example 1, and the temperature storage stability of the cosmetic was evaluated. In order to confirm the temperature storage stability, the stability of the temperature-dependent change was investigated, and the results are shown in Table 10 below.

Temperature (℃) term Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 -5 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ◎ ◎ ◎ ◎ 4 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ◎ ◎ ◎ ◎ 35 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ◎ ◎ ○ ○ 45 ℃ 1 week ◎ ◎ ◎ ◎ ◎ ◎ 1 month ◎ ◎ ◎ ◎ ◎ ◎ 3 months ◎ ◎ ○ ◎ ○ ○                      ◎: Very good ○: Good ×: Separation

From the results in Table 10, it was found that the cosmetic containing the extract of the present invention is very excellent in temperature storage stability.

Experimental Example 2: Clinical Effect of Skin Wrinkle Improvement

The clinical effect of skin wrinkle improvement on the cosmetic containing the extract of the present invention was measured.

This clinical trial divides 20 women over the age of 25 who have started or have already developed wrinkles into 2 groups, the cosmetics of Example 6 in Group A, and the cosmetics of Comparative Example 2 in Group B of the left and right eyes 12 weeks was applied. The degree of improvement of skin wrinkles was measured by classifying the objective evaluation of the expert and the subjective evaluation of the subject into the following four grades. The results are shown in Tables 11 and 12 below.

Skin wrinkle improvement evaluation criteria:

 0: no change

 1: slightly improved

 2: improve

 3: highly improved

Clinical Effects of Skin Wrinkle Improvement (Objective Evaluation by Skilled Workers) division  Skin wrinkle improvement Example 6 Comparative Example 2 Before use 12 weeks later Before use 12 weeks later Subject 1 0 3 0 0 Subject 2 0 3 0 One Subject 3 0 3 0 0 Subject 4 0 2 0 One Subject 5 0 3 0 One Subject 6 0 2 0 0 Subject 7 0 3 0 One Subject 8 0 3 0 0 Subject 9 0 3 0 2 Subject 10 0 2 0 One Wrinkle improvement average value 0 2.7 0 0.7

Clinical effect of skin wrinkle improvement (subjective evaluation of subject) division  Skin wrinkle improvement Example 6 Comparative Example 2 Before use 12 weeks later Before use 12 weeks later Subject 1 0 3 0 One Subject 2 0 3 0 One Subject 3 0 3 0 0 Subject 4 0 2 0 2 Subject 5 0 3 0 One Subject 6 0 3 0 0 Subject 7 0 3 0 One Subject 8 0 3 0 0 Subject 9 0 3 0 2 Subject 10 0 2 0 One Average value of wrinkle improvement 0 2.8 0 0.9

As can be seen from Table 11 and Table 12, in Example 6, the average value of the objective evaluation of the skilled person and the subjective evaluation of the subject was 2.7 and 2.8, respectively, which was much higher than 0.7 and 0.9 of Comparative Example 2. Therefore, it can be seen that the wrinkle improvement effect of the formulation prepared by adding the extract of the present invention is very excellent.

As described in detail above, the present invention provides a composition for inhibiting elastase and a skin wrinkle and elasticity improvement comprising the extract as an active ingredient. The extract of the present invention has excellent elastase inhibition and fibroblast proliferation effect, in particular, the fraction of water or chloroform of the extract has excellent elastase inhibitory activity. Cosmetic composition containing the extract of the present invention as an active ingredient is excellent in improving the skin wrinkles and skin elasticity, and shows excellent stability.

Claims (7)

Composition for inhibiting elastase comprising the extract as an active ingredient. Cosmetic composition for improving skin wrinkles and elasticity comprising the extract as an active ingredient. The cosmetic composition for improving skin wrinkles and elasticity according to claim 2, wherein the extract of the pacifier improves elastase inhibition and fibroblast proliferation to improve skin wrinkles and elasticity. 4. A composition according to any one of claims 1 to 3, wherein said teat extract is a water layer or a chloroform layer fraction. 5. The composition of claim 4, wherein the teat extract is a chloroform layer fraction. The composition of any one of claims 1 to 3, wherein the content of the teat extract is 0.0001-15.0 wt% based on the total weight of the composition. The composition of claim 2, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation Cosmetic composition for improving skin wrinkles and elasticity, characterized in that having a formulation selected from the group consisting of, wax foundation and spray.