KR20050091318A - A method of sprout and vegelets by chlorella and calcium ion complex - Google Patents

Abstract

The present invention relates to a sprout oil cultivation method using a chlorella and calcium ion complex, which is prepared by mixing clam shell powder, ore powder containing calcium, magnesium and silicon in an increase of 2: 8 in a primary firing at 400-500 ° C. Then, the first calcined product was immersed in 1 M (mol) sodium metabisulfate and sodium pyrophosphate solution for 3 hours, and then calcined again at 700-800 ° C. to prepare a calcium ion complex. Culturing chlorella in the culture solution by adding a liquid ratio of 0.5 to 1.0% by weight and 1.0-5.0% by weight of the calcium ion complex with respect to water, taking an aqueous solution of chlorella when the cultured chlorella concentration is 0.1-0.1% by weight and pH 8.0. Soaking seeds. Place wood chips, sawdust, etc. containing 0.05-0.1% by weight of calcium ion complexes in a box of sprout oil, spread the soaked seeds on top of them, and spray the aqueous solution of chlorella eluted with calcium ion complexes for 30 seconds every 4 hours at 25 ± 5 ℃. It is composed of the step of sprouting by shading or floodlight.

Description

Shooting method of sprout oil using chlorella and calcium ion complex {A METHOD OF SPROUT AND VEGELETS BY CHLORELLA AND CALCIUM ION COMPLEX}

The present invention relates to a seed oil cultivation method using chlorella and calcium ion complex.

In general, seeds and grains contain nutrients, hormones, etc. necessary for plant growth, and seeds and grains absorb water and nutrients from soil and water in the germination process, which is the first step to grow into plants. Germination starts when only temperature conditions are met.

Humans and animals feed or cook dried seeds and cereals to get various nutrients from them.

The process of germinating seeds or grains, that is, biosynthesis using minerals, nutrients, moisture, and nutrients stored in the seeds or grains themselves, which are absorbed from soil, water, and artificial media while the plants are 6 to 10 cm in length. As a result of this beginning, it is well known that vitamins, amino acids, hormonal substances, etc. are rapidly added. Therefore, this sprout oil is used as a variety of planting.

In the conventional sprout oil cultivation method, in order to prevent the rot disease during germination of seeds or grains, a compound fertilizer was used to treat the seeds or grains with pesticides or bacteriostatic agents and then to supply nutrients.

This causes problems such as pesticide residue, excessive organic phosphorus formation by fertilizer use, harmful heavy metals due to environmental pollution, dioxin, etc. in germination of seeds and grains. Accumulation and ingestion of these plants for a long time poses a problem for animals or humans.

In addition, chlorella was simply added or chlorella culture was used to correct the above problems during germination of seeds or grains. However, the initial germination rate and enzymatic activity are significantly lowered. There is a problem.

It is an object of the present invention to provide a seed oil cultivation method using a new chlorella and calcium ion complex which can increase the initial germination rate and enzyme activation of seeds or cereals using chlorella and calcium ion complexes.

Another object of the present invention is to remove harmful heavy metals, dioxins, etc., which may be included in general water and to prevent rot diseases, to provide high-value added agricultural products by providing pollution-free seeds and barley sprouts to contribute to increasing the disinfection of farmers. .

Generally, seeds or grains begin to germinate given conditions suitable for the germination conditions of seeds, such as moisture, temperature, and minerals. Human or animal germination plants can be divided into three stages.

First, seeds or grains start germination and sprouting is about 0.5 ~ 1.0cm.

Second, growing seeds or cereals up to 15-20 cm under two different conditions exposed to dark or bright light and using them as herbs, green young vegetables, green juice and juice;

Third, there is a step of growing into complete vegetables through hydroponic cultivation, field cultivation, and greenhouse cultivation.

Particularly, young plants that grow seeds or grains under different conditions exposed to dark or bright light, such as the first stage of sprouting of 0.5 ~ 1.0cm and the second stage, are grown by edible seeds or grains themselves or fully grown. It is richer in vitamins, minerals and phytohormone ingredients than eating ingested plants.

According to the present invention, various research experiments have been conducted on the technology of growing sprout oil in a large amount that can be industrially applied. As a result, chlorella and calcium ion complexes are used without treatment of pesticides or bacteriostatic agents harmful to humans for the prevention of rot. A new sprout oil cultivation method has been developed. In other words,

1M (mol) sodium, a food additive, to promote mineral dissolution by mixing clamshell powder and ore powder containing calcium, magnesium, and silicon in an amount of 2: 8 in an increase of 2: 8. immersed in metabisulfate or sodium pyrophosphate for 3 hours and absorbed, and then calcined again at 700-800 ° C. to prepare a calcium ion complex, followed by adding grass, seaweed and sugar to fermentation. Culturing chlorella in the culture medium by adding weight% and 1.0-5.0 weight% of the calcium ion complex to the chlorella culture medium, and seeds to germinate the chlorella aqueous solution when the cultured chlorella concentration is about 0.1-0.1 weight% and pH 8.0. Taking three times the amount of soaking seeds therein, and wood chips, sawdust, etc. containing 0.05-0.1% by weight of chlorella aqueous solution in which the calcium ion complex was eluted and placed in a box of sprout oil, Spread the immersed seeds and spraying the chlorella solution eluted with the calcium ion complex for 30 seconds every 4 hours at 25 ± 5 ℃ spraying light shading or light transmission.

According to the present invention, the germination rate of seeds and grains is significantly higher than that of the conventional one, and the enzyme activity is high, and there is no residue of harmful heavy metal dioxins, which may be contained in general water, rot diseases are prevented, minerals, vitamins, vegetable hormones, etc. You can get this rich sprout oil. Bud oil can be used as a variety of ingredients.

In addition, there is no pesticide treatment to prevent rot, and no chemical fertilizer is added. Therefore, there is no problem of pesticide residues, excessive organic phosphorus formation by using fertilizer, harmful heavy metals due to environmental pollution, dioxins, etc. These harmful substances accumulate in germinated plants by bioconcentration, and thus, pollution-free sprout oil can be obtained without harmful problems to animals or humans from ingestion of these plants for a long time.

In addition, it is possible to increase farmers' profits by creating high value added of agricultural products by cultivating seed oil such as seeds and grains.

(Example 1)

The present invention is a step of preparing a calcium ion complex, culturing chlorella in a mixture of calcium ion complex and liquid solution, soaking seeds in an aqueous solution of chlorella eluted with the calcium ion complex, sprout oil using an aqueous solution of chlorella eluted with the calcium ion complex. It consists of steps.

(1) step of preparing calcium ion complex

In the present invention, the calcium ion complex is washed with running water in the shell and dried with hot air and then pulverized to about 10 mesh.

Rocks rich in calcium, magnesium, silicon, etc. are washed cleanly, crushed into 10 mesh lengths, mixed with the coarse shell powder and the ore powder at 2: 8% by weight, kneaded by adding a little water, etc. It was shaped into granules suitable for calcination and fired for 18 hours in a 400 ~ 500 ℃ heating furnace, and then immersed in 1M (mol) of sodium metabisulfate or sodium pyrophosphate for 3 hours to promote mineral dissolution. Next, the mixture is calcined for about 4 hours in a heating furnace at 700 to 800 ° C. to obtain a calcium ion composite of the present invention.

(2) Chlorella culture step

0.5 to 1.0% by weight of the fermentation broth mixed with brown sugar in grass and seaweeds and 1.0 to 5.0% by weight of the calcium ion complex were added to the chlorella culture to elute calcium and minerals and inoculated with edible chlorella to the culture. Incubate at ± 3 ° C for 48 hours.

(3) soaking of seeds

When the cultured chlorella concentration is 0.1-0.3% by weight of water and pH 8.0, 3 times of the seeds to germinate the chlorella aqueous solution in which the calcium ion complex is eluted are immersed, and the seeds are immersed therein and immersed for 18 hours at 23 ± 3 ° C. do.

(4) sprouting stage

Into a sprout box made of plastic or wooden box having a height of 10 to 15 cm, wood chips, sawdust, etc., in which 0.05-0.1% by weight of the calcium ion complex was added, were thickened to a thickness of about 1.0 ± .5 cm Spread the seeds and cultivate sprout oil for about 24 to 48 hours while spraying the chlorella aqueous solution in which the calcium ion complex is eluted for 30 seconds every 4 hours at 25 ± 5 ° C.

The sprout oil obtained by the present invention is used as a salad or mixed with rice, barley, etc. while being stored and eaten to make grain rice.

As shown in Figure 1, in the embodiment of the present invention it can be seen that the sprouting test results using a chlorella aqueous solution in which the calcium ion complex is eluted, the germination rate is much higher than using only chlorella liquid or water.

As shown in Figure 2, in the embodiment of the present invention the test results for rooting, which is the first process of germination from seeds, is also much higher when using chlorella aqueous solution in which calcium ion complex is eluted than when using chlorella and water. Showed down.

As shown above, chlorella is important as a nutrient supplying medium because of its rich protein, growth factors, etc. In addition, calcium ion complex modifies the pH and mineral concentration to promote bacteriostatic effect, cell membrane formation, growth of myocardium, and enzyme activation.

As shown in FIG. 3, the phytase activity, which is a representative element required for plant growth, was measured, and the highest phytase activity was obtained when the chlorella aqueous solution in which the calcium ion complex was eluted was used rather than water and chlorella alone.

As shown in Figure 4, the results of the measurement of the changes in the components of the chlorella culture, glycoproteins contained in the chlorella, saccharides are rich in these chlorella growth factors, such as germination of seeds, barley, rot disease prevention due to pH alkalizing during growth, calcium It can be seen that the activation of enzymes by the ions, the cytoplasm and so on.

(Example 2)

Preparation of Green Juice, Juice and Chlorophyll Powder Using Barley Grass Oil

Barley is carried out using the soaking step of the seed of Example 1, the sprouting step.

Wood chips with 0.05 to 0.1% by weight of calcium-ion complex and sawdust are laid in a thickness of 3.0 ± 1.0cm, and the barley soaked in chlorella culture medium is laid out every 4 hours at 23 ± 5 ℃. Spray the chlorella aqueous solution in which the calcium ion complex was eluted for 30 seconds to grow for 5-7 days. When young shoots are about 5 ± 2cm long, they are exposed to light to produce chlorophyll, while growing the total length of young plants about 10 ~ 20cm in the same way as above.

Cut the sprouts and mix them with juice to drink or mix with green juice. Mix with young leaves of barley or with other young shoots, freeze-dried and prepared as chlorophyll powder.

Although barley sprout oil has been described in the present embodiment, various grains or seeds may be applied.

In the present invention, since seeds and cereals are sprouted using chlorella and calcium ion complexes, it is possible to obtain seeds or barley sprouts rich in vitamins, minerals, and functional substances, compared to general plants.

In addition, it is rich in chlorella protein and growth factors, and calcium ion complex improves bacteriostatic effect, cell membrane formation, growth of myocardium and enzyme activation by modifying pH and mineral concentration, so it has high initial germination rate, good sprout oil and rot disease. You can prevent it.

In addition, there is no pesticide treatment to prevent rot, so there is no problem of pesticide residues, excessive organic phosphorus formation by using fertilizers, harmful heavy metals due to environmental pollution, dioxins, etc. during germination of seeds or cereals.

In addition, conventional harmful substances are accumulated in the germination plants by the bioconcentration action, there is no problem that harmful to animals or human body when ingesting these plants for a long time.

Therefore, according to the present invention, various seed oils, which are rich in nutrients and functional substances, can be cultivated in large quantities in a pollution-free manner and can be provided as useful new healthy foods.

1 is a comparison table of barley germination rate of chlorella, water, chlorella and calcium ion complex

Figure 2 is a comparison table of barley roots of chlorella, water, chlorella and calcium ion complex

Figure 3 is a comparison table of phytase activity in shoot barley by chlorella, water, chlorella and calcium ion complex

Figure 4 is a table of changes in the components of chlorella culture solution eluted calcium ion complex

Claims (2)

Clamshell powder, ore powder containing calcium, magnesium, silicon, etc. are mixed at 2: 8% by weight to form granules, which are calcined at 400 to 500 ° C for 18 hours, and then 1M (mol) as a food additive. Immersing in Sodium metabisulfate, Sodium pyrophosphate solution for about 3 hours and calcining at a temperature of 700 to 800 ° C. for 4 hours to prepare a calcium ion complex, A chlorella culture step of inoculating edible chlorella in a chlorella culture solution to which a liquid ratio of 0.5 to 1.0 wt% and 1.0 to 5.0 wt% of the calcium ion complex was added thereto, and culturing at 20 ± 3 ° C .; When the cultured chlorella concentration is 0.1-0.3 wt% of water and pH 8.0, take chlorella aqueous solution, add seeds and soak for 18 hours at 23 ± 3 ℃, Spread the wood chips and sawdust mixed with 0.05-0.1% by weight of the calcium ion complex in a seedling box, spread out the immersed seeds, and spray the chlorella aqueous solution in which the calcium ion complex was eluted every 25 hours at 25 ± 5 ° C. Sprout cultivation method using chlorella and calcium ion complex, characterized in that consisting of oil phase. The method of claim 1, wherein the barley soaked in an aqueous solution of chlorella eluted with the calcium ion complex in the soaking step of the seed is squeezed over the wood chips and sawdust spread in the box of seedlings in the seeding stage, and then calcium ions at predetermined times at 23 ± 5 ° C. Aqueous solution of eluted chlorella was grown for 5-7 days and exposed to light when the length of barley shoots was about 5 ± 2cm to produce chlorophyll. Sprout oil cultivation method using chlorella and calcium ion complex, characterized in that the chlorophyll powder.