KR20020013055A - Therapeutic effect of bee venom in calves with bacterial diarrhea - Google Patents

Abstract

PURPOSE: A method for treating calf diarrhea by applying poison of Apis mellifera as a milkman bee to a specific acupoint of a calf having diarrhea is provided, thereby causing no problems of antibiotic resistance and accumulation in the body and improving therapeutic effects. CONSTITUTION: The treatment method for calf diarrhea comprises: seizing an Apis mellifera L. one by one with a pincette; allowing the seized Apis mellifera L. to sting a specific acupoint of a calf having diarrhea such as kyuoso-hyeol and baekhoe-hyeol associated with the treatment of calf diarrhea; and allowing them to sting an auxiliary acupoint of the calf such as haemun-hyeol associated with the treatment of calf diarrhea to increase treatment effects. This method for treating calf diarrhea using bee venom exhibits a high therapeutic effect of 81.2%.

Description

Therapeutic effect of bee venom in calves with bacterial diarrhea}

The present invention relates to the treatment of bacterial diarrheal diseases of calves, and more particularly to the treatment of diarrheal diseases of calves by using live bee venom, which is a natural animal physiologically active substance, to specify the acupuncture point corresponding to the treatment of calves diarrhea and By inducing live bee venom to be injected naturally by directly contacting the bee stings of living bees to the blood level, it is related to the treatment of diarrheal diseases of calves, which is easy to carry out the procedure, has high therapeutic effect, is economical, and does not have antibiotic resistance and residual accumulation in the body.

In general, diarrhea is the most characteristic symptom of enteritis that causes the bowel or colon to malfunction due to frequent excretion of feces containing excess water compared to normal. Intestinal mucosa, responsible for absorption and secretion of serous components, causes lesions on the membranes, resulting in poor absorption of feed and water ingested by animals or excessive secretion of serous components.

Diarrhea is a type of biological defense mechanism that attempts to protect the living body by quickly releasing harmful substances generated in the digestive tract or from outside. Therefore, if the toxin from outside or enter the intestinal tract is not excreted in vitro by diarrhea, it is absorbed, causing more systemic pathological conditions. However, when severe diarrhea continues, the absorption of nutrients ingested is not only impeded, but also the loss of water and electrolytes in body tissues, followed by dehydration and electrolyte imbalance. In this state, physical metabolic activity is not normal. The result is.

In fact, the death of livestock due to diarrheal disease is mostly due to secondary dehydration rather than diarrhea itself. For this reason, so-called fluid therapy to replenish lost fluids and electrolytes is more important than antibacterial therapy to remove diarrhea. Bacterial diseases caused by diarrhea due to enteritis or malabsorption include Escherichia coli, Salmonella, Clostridium perfringens type C infection, and proliferative enteropathy (ileitis).

Calf diarrhea is one of the most common diseases in cattle farms, whether dairy or fattening. Compared to the voice actors, the calf has low resistance, so diarrhea occurs even if the specification management is a little wrong, so it is easy to delay or die due to diarrhea.

As a method for treating calves' diarrhea, a drug therapy using a syringe and injectable drugs is common, and recently, an immunoacupuncture method for injecting a drug into the acupuncture point of the calf has been developed. Drug therapy or acupuncture is the administration of artificial synthetic drugs in the treatment of calf disease. There is a problem in that antibiotics and anti-diarrheal drugs must be administered continuously for two to three days from the onset of onset, and in the long term, resistance to drugs There was a flaw such as increasing the slow diarrhea recovery rate, the cost of treatment required for drug administration.

The present invention has been made to solve the conventional problems as described above, an object of the present invention is to specify the bee acupuncture points of calves in accordance with the treatment of diarrheal diseases of calves using live bee venom that is a natural bioactive substance of animal, not synthetic drugs By direct acupuncture of living worker bees on the bloodstream to induce the natural bee venom to be injected naturally, the procedure is simple, highly effective and economical treatment of bacterial diarrheal disease of calves.

1 is a diagram showing the live bee venom bee stroma during bacterial diarrhea of a calf according to a preferred embodiment of the present invention.

In order to achieve the above object, the present invention,

Preparing a surgical rod in which the raw rod is accommodated (S1);

Capturing live rods with tweezers one by one from the surgical rod (S2);

Injecting the captured live bee venom into the main blood acupuncture points associated with the treatment of diarrheal diseases of calves, glioblastoma and leukemia (S3); And

In order to promote the treatment of diarrheal disease, the step of injecting live bee venom into the haemunhyeol (海 인), a subsidiary bee venom associated with the treatment of diarrheal disease (S4) comprising the treatment of bacterial diarrhea disease of calf using live bee venom to provide.

Preferably, the live bee is a Western species honey bee using a mature worker bee 15 days or more after discharge.

Hereinafter, a method for treating bacterial diarrhea of a calf using live bee venom according to the present invention will be described in detail.

In the present invention, animal bee venom, which is a natural physiologically active substance, is used to treat diarrhea of calves. That is, to treat diarrhea of calves, we try to cure diarrheal diseases by directing the bee sting of living worker bees on the bee stings of calf, which corresponds to the treatment of diarrhea.

To achieve this, the composition and content of the raw bee venom used in the present invention is as shown in Table 1 below. That is, there are 11 peptide components, 5 enzyme components, 3 bioactive amine components, and 4 non-peptide synthetic substances, and 23 components have been analyzed to date (Christopher Kim, 1992). In this way, raw bee venom is mixed with a variety of substances, the most abundant ingredient in dry bee venom is merittin (melittin) accounting for 40 to 50%.

Raw bee venom has a strong bitter taste and a unique scent. The pH of raw bee venom is 5.2-5.3 and its specific gravity is 1.1313. Raw bee venom is rapidly evaporated when it is released to room temperature, leaving about 30 to 40% of crystals.

Table 1. Contents of Raw Bee Venom by Components

system Ingredient Content (% building) Peptides (11 types) Merittin apamin MCD-peptide (MCD-peptide (peptide 401)) adorapin (protease inhibitor secapin tertiapin tertiapin F) procamine A (B) minimine cardiopep 40 to 502 to 32 to 31 <0.80.50.10.011.42 to 3 <0.7 Enzyme (five kinds) Bahia ruro The first (hyaluronidase) phospholipase A ₂ (phospholipase A ₂₎ alpha-glucosidase (α-glucosidase) Acid phosphonate mono esterase (acid phosphomonoesterase) Li parcel spokes lipase (lysophospholipase) 1.5-210-120.61.01.0 Physiologically active amines (3 types) Histamine dopamine norepinephrine 0.6 to 1.60.13 to 1.00.1 to 0.7 Non-peptide Ingredients (4 Types) Carbohydrates (glucose and fructose) lipids (6 phospholipids) amino acids (γ-aminobutyric acid β-aminoisobutyric acid) <24 to 5 <0.5 <0.01 system 23 kinds

When the live bee venom having the components of Table 1 is injected into the body tissues of mammals, each component acts collectively to stimulate the immune system of the mammal, anti-inflammatory, cell lysis (hemolysis), neurotoxic effect, anti It has been found that pharmacological functions such as bacterial and antifungal action, radiation protection action, and antialkylation action are combined.

The composition of each component of the live bee venom and the activity in mammalian biological tissues revealed so far are shown in Table 2 below (Christopher Kim, 1992).

Table 2. In Vivo Actions of Raw Bee Venom by Component

ingredient Pharmacological Operations References Merittin Cytolytic action (white blood cells, mast cells, lysosomes, mitochondria), anti-inflammatory action, immune action Jentsch (1969) Apamin Neuralgia relief, analgesic, anti-inflammatory, immune, neurotoxic Harbermann et al. (1965) MCD-peptide 401 Anti-inflammatory action Hanson et al. (1974) Adorapin Anti-inflammatory, analgesic, antipyretic Shkenderov (1982) Protease inhibitor Protein and ester dissolution inhibitory effect, anti-inflammatory action Shkenderov (1973) Secapin Hypothermia, sedation Gauldie et al. (1976) Tertiapin Mast Cell Degranulation Gauldie et al. (1978) Procarmine (procamine A, B) Related to radiation protection Peck et al. (1978) Hyaluronidase Histolytic activity, antigenic component Baker (1966) Phospholipase A ₂ (phospholipase A ₂₎ Destructive, Hemolytic, and Catalytic Activity of Cell Tissues Jentsch (1972) Alpha-glucosidase Antibody Role Enhancement Shkenderov et al. (1979) Acid phosphomonoesterase Antibody Role Enhancement Benton (1965) Lysophospholipase Inhibition of Phospholipase A ₂ Action Doery (1964) Histamine Blood pressure strengthening, intestinal contraction Owen (1974) Dopamine Neurotransmitter Owen (1971)

As mentioned above, since the pharmacological treatment effect and immune function of live bee venom are excellent in mammals in vivo, the present invention intends to shorten the treatment time by easily injecting live bee venom according to the disease. In addition, to improve the immunity before the occurrence of the disease to prepare a method for preventing the disease.

On the other hand, the injection dose of live bee venom can be determined in the case of an extracted dry live bee venom, but it is difficult to accurately measure the amount of bee venom injected directly into the body tissue of the animal by live bees. Therefore, in the present invention, the injection amount of the live bee venom solution is determined on the basis of the number of procedures on the basis of the average live bee venom amount per worker bee is 0.03 mg.

According to Christopher Kim (1992), the maximum number of live bee venom injections for humans weighing 70 kg is limited to 20 animals per day. It is about 0.6 mg. Animals have a shorter lifespan than humans and have a low resistance to various types of drugs while living. Therefore, even if a small amount of live bee venom is injected than humans, the therapeutic and preventive effects are high.

Live bee venom treatment requires the selection and collection of bee for live bee venom therapy. There are many varieties of honeybees from all over the world. The honeybees that are bred in our country are the Oriental species (the native species) and the Western species, and most of them are Western species. Native bees can be used for the treatment, but the conditions for breeding are more demanding than the western species, and their environmental adaptability is weakened. On the other hand, Western bees have high popularity, easy specification management, and high breeding ability, so that they can be sufficiently used for treatment in farms by maintaining strong group. In addition, it is highly adaptable to the domestic environment, and adapts well even when the breeding place is moved, and it is safe to manage due to its weakness. In addition, they are milder than conventional species and have higher wintering capacity. Therefore, in the present invention, live bee venom therapy is performed using Western bee.

There are three kinds of western bees: queen bee, squirrel and worker bees. Queen bees have poison sac and bee sting, but there is only one bird in the group of rods. Worker bees continue to breed from spring to autumn, keeping about 20,000 worker bees per group of beekeepers (single-phase 10-group), with venom sacs and bee stings, and can be easily captured. .

Among the worker bees, there is a difference in the storage of live bee venom in the venom bag. The bee for injecting live bee venom is preferably used to capture only mature worker bees 15 days or older after discharge. This is because mature bees 15 days of age or older after being released have a strong bee sting and are well infused with live bee venom, and a lot of live bee venom is injected. In worker bees over 15 days of age, about 0.02 to 0.04 mg (average 0.03 mg) of live bee venom is stored in the sac. When stimulated, the bee sac is contracted by neural action, and the bee venom is secreted to the outside through bee sting. After 17 days of age, the production capacity of live bee venom almost ceased and the weight of the venom sac remained unchanged.

Among the worker bees for treatment, the external features of the bong are relatively large, with no hair and gloss in the head, chest and abdomen. The overall body color is dark blackish brown, and the property is sensitive to external stimuli, so it has a strong attack. The more the beebong is stronger, the better the infusion of live bee venom and the penetration of body tissues.

For the treatment of live bee venom, worker bees must be caught more than 15 days of age. The life span of worker bees is not constant and varies depending on the season and labor force. In the spring and autumn, the bees' activity reaches its peak, and its life span is about 1 to 2 months. In the July-August season, it is about 1 month to promote metabolism for temperature control. Winter worker bees live for about 4 to 6 months because of their low physical consumption.

When capturing the raw rods for a procedure, a tweezer for a procedure and a portable beekeeper are needed. The tweezers use a strong point with a sharp tip and a slightly flattened tip. In addition, the beekeeper for the procedure is a small transparent plastic box of about 11 cm in width, 5 cm in height, and 6 cm in height. Beekeepers can house more than 250 Western species of bees, and the bottom has food containers for food. If bubble fed sugar is put into the feeding container, worker bees use it as food. If food does not fall in the beekeeper, worker bees can survive for about 10 to 20 days. In a beekeeper, a bee can survive without supplying water.

Tips for capturing raw rods for treatment vary by season.

First, in the season when worker bees are not active outside (from late autumn to early spring), bee needles are carefully used in the Bonsang group under the condition that the static state of the ball is not changed to dynamic. Capture with tweezers. In more detail, a beeper lid is opened in a quiet manner and when the raw rods are captured, the bee's legs are grasped with tweezers for use on the surgical rod.

Second, when there are not many bees that act in front of the rumors early in the morning or just before sunset, the worker bees quietly deviate left or right around the front of the beekeeper and then use the prepared tweezers. Hold the leg and carefully insert it into the surgical rod. Next, when worker bees enter and exit through the rumors of the rod (April to November of the bee's active season), put the bubble sugar into the rod for feeding and remove the rubber band of the rod for a while. Open the door of the dragon statue about 1/2 to 3/4 and place the surgical rod opened in slow motion perpendicular to the rumors of the group. After 10 to 20 minutes in this state, about 50 to 300 worker bees enter the procedure bar without any impact on their own.

As mentioned above, the trapping of worker bees should always be done with tweezers. Worker bees can survive long periods of time without physical damage (more than 20 days). If the abdomen, head, and chest are captured with tweezers, part of the body tissue is injured, so worker bees are killed within a short time, so the treatment of live bee venom using live rods is difficult.

Food is needed to survive the worker bees captured into the surgical rod for a long time, and suitable food is bubble solid sugar. If you put this bubble sugar on a surgical rod, even if it is stored for more than 10 days, worker bees suck it as a substitute for food, and thus the survivability is extended, thereby increasing the convenience of the surgical bee venom for the procedure. At this time, when liquid or complete high sugar is liquefied and buried in the body of the bee, the chest and abdominal pores of the bee are blocked, which shortens the life of the bee and causes death.

On the other hand, worker bees are exposed to temperatures below 5 ℃, viability is lowered due to low temperature shock, beekeepers should always keep above 5 ℃. In particular, if you do raw bee venom injection at sub-zero temperatures in winter, warmth is essential. Worker bees trapped on the surgical rod during the hot summer heat should be stored in a cool place to avoid long-term survival. In particular, if the hot summer direct sunlight or put in the car fatality proceeds quickly. In addition, bees are very vulnerable to insecticides, so insecticides such as mosquitoes or mosquitoes should always be isolated and disposed of.

In all cases, surgical rods are placed in air-tight dark guard nets or paper bags that are not covered with vinyl to inhibit bee activity when not in use to prolong life.

The present invention is based on the body blood counts of calves, which have been proposed in heavy veterinary medicine (Jung Jung Il et al., 1992) and calf acupuncture treatment (Lee Jong-chang, 1979) to treat bacterial diarrheal diseases of calves using live bee venom. Bacterial diarrhea was selected as a bee venous blood infusion of live bee venom.

1 is a view showing the live bee venom beetle at the time of bacterial diarrhea according to a preferred embodiment of the present invention. According to a preferred embodiment of the present invention, as shown in Fig. 1, the calf is subjected to live bee venom therapy based on the degree of blood acupuncture of diarrhea.

Hereinafter, a method of injecting live bee venom from bacterial diarrhea will be described with reference to FIG. 1. Bacterial diarrhea is infused with live bee venom on the bee stings of calves that are compatible with treatment.

To this end, first, a surgical rod containing the raw rods is prepared, and the raw rods are captured by tweezers one by one from the prepared rods. In other words, open the door of the surgical rod containing the bee a little bit to capture the bee with tweezers, in this case grab the body of the bee (chest or abdomen) is taken out. After capturing the raw rods as described above, the raw bee venom is treated according to the diarrheal disease-treated acupuncture point (= FIG. 1) according to a preferred embodiment of the present invention.

First, in the first stage of infusion of live bee venom, live bee venom is injected into glioblastoma blood and white gray blood, which are the main bee venous blood vessels associated with bacterial diarrheal disease. At this time, the glia blood is a recessed portion of the lower back of the anus, and the blood that manages the calf's genital disease, digestive diseases, and the like. In addition, the white blood is the central position of the recessed portion in the midline between the lumbar spine and the sacral spinous process. The number of injections of live bee venom depends on the progress of diarrheal disease and the calf's weight.

Infusion of live bee venom In the second step, live bee venom is injected into Haemunhyeol (海 門 穴), which is a supplementary bee venous level associated with diarrheal disease, in order to enhance the treatment and prevention effect of diarrhea. In this case, haemun blood is the blood that governs the calf's digestive system, and the left and right sides of the navel are 1 to 2 cm apart, and live bee venom is treated to haemun blood.

On the other hand, the treatment of live bee venom is selected or used in combination with a live rod direct injection method, abdominal cutting injection method.

Direct rod injection is a procedure in which the raw rods taken directly out from the procedure rod with tweezers are needled directly on the acupuncture point of the disease to be treated. Neural reactions attached to the live bee venom continues to be released into the tissues. Penetrated needles penetrate into body tissues do not spontaneously remove themselves after a certain period of time.

Next, the abdominal cutting injection procedure removes the head and chest of the rod and removes the rod from the rod, holding the wing behind the bee's chest with two tweezers or the thumb and index finger of the other hand for convenient treatment with tweezers. Only the attached abdomen is separated immediately before the procedure, and then acupuncture directly on the acupuncture points. When the bee needle penetrates into the body tissues by contraction of the abdomen, the abdomen is separated and only the needles remain in the body tissue to inject bee venom into the tissue.

If the diarrheal disease is not completely cured after injecting the live bee venom, repeat the live bee venom procedure in the same way. In other words, in order to shorten the treatment time or promote a therapeutic effect after injecting live bee venom, a second infusion is attempted at the same blood level as the first treatment position according to the condition. Then, diarrheal disease is effectively treated.

<Example>

Materials and methods

(1) a public animal

From March to August 1999, 124 cows and cows infected with bacterial diarrhea among 40-92-day-old calves raised in farms in Cheongju-si and Cheongwon-gun, Chungcheongbuk-do were included as official animals. The animals were examined only for calves with 7% or less dehydration.

(2) Raw bee venom and drug administration

Diarrhea treatment was divided into live bee venom treatment group and control group drug treatment group. The live bee venom group took the western bees with tweezers for surgical treatment, and squeezed one straight bee on the 交 巢, 百 會 and 海 門 각각 for three consecutive days. If there were watery feces on the bee sting blood level, they were washed with warm water, and the raw rods were spit. In the control group, ciprofloxacin (ciprotyl ^?? , 2.5 mg / kg, Eagle Chemical Co., Ltd.) and berberine chloride (kingbellin ^?? , 10 mg / head, Samyang Pharmaceutical Co., Ltd.) were injected intramuscularly for 3 consecutive days.

(3) the selection of medicines and raw materials

Blood pressure was collected using acupoints detector (CS-202A, Kanaken, Japan) for the detection of blood, urine, and sea, which is recommended as an effective blood level for the digestive function of animals. The worker bees of Western species, Apis mellifera L. , were slightly different in their bee venom stocks according to their age, and were selected according to their appearance. In this experiment, mature bees were selected for more than 15 days after their release.

(4) fecal examination

Diarrhea in calves was placed in specimen cups and examined for causative agents of diarrhea with an automatic bacterial identifier (bioMerieux Vitek, WSVTK-R05.04, USA). Separation of bacteria in a complex form was caused by 90% or more bacteria.

<Results and Discussion>

Clinical symptoms of convulsions

There were individual differences according to the degree of diarrhea. The severe calves of the diarrhea were separated from the herd, slow in motion and lacking in energy. In addition, the elasticity of the skin was reduced, and dehydration caused ocular depression and dry mouth mucosa. Severe cases showed systemic dehydration, loss of appetite, and abnormal gait, and decreased body temperature and collapse.

In gross findings, calves with less than 7% dehydration hold the abdomen of the live rod with surgical tweezers and direct acupuncture to the glia, white ash and haemun blood, and the bee is injected into the calf's body by the abdominal contraction. It became. When bee venom was injected, the calf crumbled slightly and the appetite decreased slightly depending on the individual.

Therapeutic Effect of Diarrhea

In calves with bacterial diarrhea, E. coli was the most common, followed by Salmonella, Staphylococcus spp. Streptococcus spp. It was detected in this order. The treatment of live bee venom was found in Staphylococcus spp. And Streptococcus spp. This showed a good effect (see Table 1 below). The treatment rate of the group treated with bee venom was 81.2%, which was higher than that of the drug group (76.4%) (see Table 2 below).

Table 1.Therapeutic effect of bee venom in calves with bacterial diarrhea

Bacteria Incidence (head) Recovery (head) Recurrence (head) Therapeutic rate (%) E. coli 17 14 3 82.4 Streptococcus spp. 7 6 One 85.7 Staphylococcus spp. 11 10 One 90.9 Salmonella 13 11 2 84.6 Corynebacterium 5 3 2 60.0 Proteus 5 4 One 80.0 G (-) bacillus 3 2 One 66.6 Other 8 6 2 75.0 Total 69 56 13 81.2

Table 2.Therapeutic effects of ciprofloxacin and bee venom in calves with bacterial diarrhea

Group Incidence (head) Recovery (head) Therapeutic rate (%) Ciprofloxacin-treated 55 42 76.4 Bee venom-treated 69 56 81.2

Prophylactic methods of diarrhea of calves should be carried out first. If the cause is non-infectious, the cause should be eliminated, and if it is bacterial infectious, the drug should be selected according to the antibiotic susceptibility.

Experimental treatment of neomycin in calves with E. coli artificially infected with diarrhea caused 71.4% of treatments, but 81.8% of acupuncture treatments. Calves affected by acupuncture diarrhea were also treated. Acupuncture stimulation of the blood (GV-1) was effective in treating constipation as well as diarrhea. Anatomically, the nerves from the spinal cord form ganglions and re-distribute to each visceral organ. The sympathetic and parasympathetic nerves are distributed to control the motility of the intestinal tract. It is assumed to stimulate. It is believed that acupuncture treatment of glioblastoma in diarrhea promotes the secretion of endogenous opiod peptides, especially endorphins and enkephalins, which not only activate the immune system but also cause complex healing mechanisms such as relaxation of intestinal motility. Acupuncture therapy for infectious diseases is known to activate the immune system and have antiviral effects such as antibody formation, leukocyte phagocytosis, antibacterial and anti-inflammatory effects.

In herbal medicine, there is a new method of 水 針 療法, which is also called 血 位 注射 療法, 中草藥 注射 療法, or 藥 針 療法, and when using intramuscular or intravenous injections using 질병 or 하고 and using acupuncture methods Treating could increase the therapeutic effect. Before weaning, 2ml of 3% physiological saline was injected into calves infected with diarrhea, which shortened the duration of disease treatment compared to the control group administered 0.5g lactose.

In the present invention, as a result of treatment of bacterial diarrhea of calf by injecting live bee venom from Western bees by selecting glia and leukemia as the main bloods and haemophilia as the secondary blood of animals corresponding to human intestinal blood, staphylococcus and streptococci Calves infected with, Salminella and Proteus showed high therapeutic effects. These results show that melittin has a strong antibacterial and antifungal effect among bee venom components. The antibacterial action was more effective against gram-positive bacteria than gram-negative bacteria, about 1,000 to 1,200 times stronger than penicillin, and Staphylococcus aureus strain resistant to penicillin. It worked for 80. The antibacterial mechanism of bee venom is that melittin has a high affinity for bacterial cell membrane lipids and is translocated through holes in the cell membrane. In addition to antibacterial activity, bee venom has been shown to have various physiological effects such as anti-inflammatory, sedative and analgesic effects. Bee venom stimulates the pituitary gland and adrenal glands directly and indirectly to secrete cortisol for anti-inflammatory action. This effect has been demonstrated to be anti-inflammatory in the aseptic wounds of dogs with aseptic surgery.

Bee venom showed higher therapeutic effect than pharmacotherapy in the treatment of calves with bacterial diarrhea. However, in the gross findings, calves dehydrated more than 7% required fluid therapy in addition to bee venom. Livestock death due to diarrhea was most often due to secondary dehydration rather than diarrhea itself. Therefore, it was judged that the treatment of diarrhea was more important than the antimicrobial therapy, and fluid therapy supplemented with lost water and electrolytes.

In the treatment of bacterial diarrhea of calf, live bee venom therapy was better than drug therapy with antibiotics and anti-diarrheal drugs, and side effects such as allergy, poisoning, bleeding and infection were not observed in calf treated with live bee venom. It could be seen that it could be an alternative.

<conclusion>

In order to investigate the therapeutic effect of bee venom ( Apis mellifera L. ) on the live bee venom in calves affected with bacterial diarrhea, it was divided into the live bee venom treatment group and drug administration group. The live bee venom treated groups of bees of western bees, 15 days after their release, were placed on the calves, calves, and haemunhyeol for three consecutive days. Ciprofloxacin (ciprotyl ^?? , 2.5 mg / kg, Eagle Chemical Co., Ltd.) and berberine chloride (Kingbelen ^?? , 10 mg / mari, Samyang Pharmaceutical Co., Ltd.) were administered intramuscularly once a day for 3 days.

The drug group showed 76.4% of the treatment, and the live bee venom group showed 81.2% of the treatment. As a result of administering live bee venom to calves infected with bacterial diarrhea, allergy, side effects such as intoxication, bleeding and infection did not appear, and it could be seen that it could be a new alternative to the treatment of bacterial diarrhea.

Bacterial diarrhea treatment according to the present invention as described above, in the treatment of diarrheal disease of calves, the bee sting of living bees on the main bee sting and the bee stings in accordance with the treatment of diarrheal disease to induce live bee venom is naturally injected. . Therefore, in the present invention, since the existing antibiotics are not used to treat calf bacterial diarrheal disease, there is little need to worry about the resistance of antibiotics and residual accumulation in the body, the treatment effect is high, and the cost of treating diarrhea (drug cost, veterinarian cost) It can reduce the cost and produce very safe and clean pork.

Although the above has been described with reference to a preferred embodiment of the present invention, those skilled in the art will be able to variously modify and change the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated.

Claims (4)

Preparing a surgical rod in which the raw rod is accommodated (S1); Capturing live rods one by one with tweezers on the surgical rod (S2); Capturing the raw rods into glioblastoma blood and white blood blood, which are the main bony acupuncture points associated with the treatment of diarrheal diseases of calves (S3); And A method of treating diarrheal diseases of calves using live bee venom, comprising the step (S4) of applying live rods to haemunhyeol (海 門 穴), which is an auxiliary bee-streaking position related to the treatment of diarrheal diseases, in order to increase the therapeutic effect of the diarrheal disease. The method of claim 1, wherein the step (S3) and the step (S4) is characterized in that the raw rods are centered on the main and acupuncture points in parallel with one of the direct injection method and the abdominal amputation injection method. Diarrheal disease treatment of calf using live bee venom. The method of claim 2, wherein the direct rod injection method is a procedure in which a raw rod taken out of the surgical rod with a tweezer for needles is directly acupunctured on the main needle acupuncture point and the auxiliary rod needle acupuncture point. Only the body of the rod is removed in the state of being embedded in the body tissue, and the abdominal cutting injection method, holding the back wing of the chest of the rod with the thumb and index finger of the other hand of the operator to remove the rod with the tweezers from the surgical rod The head and chest of the raw rods are removed for convenient treatment with tweezers, and only the abdomen with the needle is separated immediately before the procedure, and the needle is needled directly on the main and acupuncture points. Once penetrated into the body tissue, the abdomen is separated and only the bee sting remains in the body tissue to inject bee venom into the body tissue. Diarrheal disease treatment of calf using live bee venom, characterized in that. The method of claim 1, wherein the raw bee is a mature worker bee at least 15 days of age after discharge from a Western bee.