KR19990037903A - Immunization therapy of piglet by honeybee venom - Google Patents

Abstract

In order to enhance the immunity of new piglets, it is possible to use animal bee venom, which is a natural functional substance of animal origin, not artificial synthetic material. Live bee venom is infused naturally by injecting live bee stings into the bloodstream, and the treatment is simple, high therapeutic effect and economical. According to the present invention, for the purpose of immunization of piglets immediately after delivery, an Italian honeybee was prepared for a surgical rod containing a mature worker bee 15 days or older after discharge, and captured live rods with tweezers one by one from the prepared surgical rod. Next, the captured live bee venom is injected into haegyeol blood (hae 門 穴), glioblastoma and head blood (?? 口 穴), which is a bee acupuncture point corresponding to the immune boosting of newborn piglets.

Description

Immunization therapy of piglets using live bee venom {Immunization therapy of piglet by honeybee venom}

The present invention relates to immuno-stimulation of pigs, and more particularly, by using live bee venom, an animal natural functional active substance, to enhance the immunity of newborn piglets immediately after delivery, and to identify a bee-cup acupuncture point corresponding to the immune promotion of newborn piglets ( By specifying and directing the bee stings of living worker bees to this site, the natural bee venom is infused naturally, thereby simplifying the procedure, enhancing the immune effect of new piglets, and economically improving immunity of new piglets.

Various drugs have been developed and used to treat inflammatory diseases of pigs, which are difficult to treat. However, the resistance to drugs has increased, and the recurrence has also increased remarkably. Therefore, it takes a lot of time, treatment cost, and effort to treat swine disease, and the proportion of prevention and treatment costs in management costs is gradually increasing. In addition, the administration of these drugs lowers the quality of pork and ultimately seriously threatens the health of humans eating pork.

In general, prevention of disease includes prevention of pathogens such as bacteria, viruses, fungi and parasites, or prevention of these pathogens from accessing the affected animals. Countermeasures include antibody formation by vaccination. Vaccination weakens or kills pathogens and inoculates animals to gain immunity, a defense against disease.

Drug treatment using a syringe and injectable medicine is generally used as a treatment for the disease, and recently, acupuncture treatments for injecting drugs into human acupuncture points have been developed. Drug therapy or acupuncture is the administration of artificial synthetic drugs in the treatment of disease.

For example, in conventional hog farms, there is no special method for the immune boosting of newborn piglets after delivery, and it has relied heavily on sows colostrum. Immediately after delivery, the new piglets must be delivered from colostrum to produce their own immune material independently from the mother, and it is important to promote digestion to digest the colostrum consumed by themselves. By the way, in the case of newborn piglets are usually severely injured, incised, subjected to castration, severe wounds and stress and digestive capacity is reduced. Accordingly, antibiotics have been conventionally administered to help the recovery of such trauma and the recovery of stress. As a result, as mentioned above, the resistance to the drug was increased, the recovery of wounds and stress was slow, the quality of the pork was lowered, and the cost of treating the drug was high.

The present invention has been made to solve the conventional problems as described above, the object of the present invention is to use the live bee venom, which is a natural functional active substance of animal, not a synthetic drug to the bee acupuncture points of pigs corresponding to the immune promotion of newborn piglets By stinging the bee stings of living worker bees to induce the natural bee venom to be naturally injected, the procedure is simple and provides high immunity and economic feasibility.

1 is a view showing a live bee venom beetle to increase the immunity of newborn piglets according to a preferred embodiment of the present invention.

<Explanation of symbols for main parts of drawing>

10: haemun blood (海 門 穴) 12: gyomo blood (交 巢穴)

14: head blood count (?? 口 穴)

In order to achieve the above object, the present invention,

Preparing a moving rod for a procedure in which the raw rod is accommodated (S1);

Capturing live rods by tweezers one by one from the surgical rod (S2); And

Injecting the captured live bee venom into the haenggeom blood (hae 門 穴), gliosis and head blood (?? 口 인) that is a bee acupuncture point corresponding to the immune boosting of newborn piglets (S3) characterized in that it comprises It provides a method for improving the immunity of newborn piglets using live bee venom.

Preferably, the live bees are mature species of bees 15 days or older after release as Italian species bees.

Hereinafter, an immunopromoting method of newborn piglets using live bee venom according to the present invention will be described in detail.

In the present invention, live bee venom, which is a natural animal active substance, is used to boost piglets immediately after delivery. In other words, live bee venom is infused naturally by directing the bee sting of living worker bees on the bee sting blood of pigs corresponding to the immune boosting of newborn piglets. He wants to recover quickly and improve the pig's immunity.

Table 1. Content of Bee Venom by Component

System Composition Content (% building) Peptides (11 types) Merittin apamin MCD-peptide (MCD-peptide (peptide 401)) adorapin (protease inhibitor) secapin tertiapin tertiapin melittin f (melittin) F) procamine A (B) minimine cardiopep 40 to 502 to 32 to 31 <0.80.50.10.011.42 to 3 <0.7 Enzyme (five kinds) Bahia ruro The first (hyaluronidase) phospholipase A ₂ (phospholipase A ₂₎ alpha-glucosidase (α-glucosidase) Acid phosphonate mono esterase (acid phosphomonoesterase) Li parcel spokes lipase (lysophospholipase) 1.5-210-120.61.01.0 Bioactive amines (3 types) Histamine dopamine norepinephrine 0.6 to 1.60.13 to 1.00.1 to 0.7 Non-peptide Ingredients (4 types) Carbohydrates (glucose and fructose) lipids (6 phospholipids) amino acids (γ-aminobutyric acid β-aminoisobutyric acid) <24 to 5 <0.5 <0.01 system 23 kinds

Table 1 shows the composition and content of raw bee venom used in the present invention.

That is, 11 kinds of peptide components, 5 kinds of enzyme components, 3 kinds of physiologically active amine components, 4 kinds of non-peptide synthetic substances, and 23 kinds of components have been analyzed to date. In this way, raw bee venom is mixed with a variety of substances, the highest content of dry bee venom components are merittin (melittin) occupies 40 to 50%.

When bee venom having the components of Table 1 is injected into the body of a mammal, each component acts collectively to stimulate the mammalian immune system, anti-inflammatory action, cell lysis (hemolysis), neurotoxic effect, antibacterial And pharmacological functions such as antifungal action, protection from radiation, and antialkylation action have been found to be combined.

Table 2. In Vivo Activity of Major Components of Bee Venom

ingredient Pharmacological Operations Merittin Cytolytic activity (white blood cells, mast cells, lysosomes, mitochondria), anti-inflammatory, immune Apamin Neuralgia relief, analgesic, anti-inflammatory, immune, neurotoxic MCD-peptide 401 Anti-inflammatory action Adorapin Anti-inflammatory, analgesic, antipyretic Protease inhibitor Protein and ester dissolution inhibitory effect, anti-inflammatory action Secapin Hypothermia, sedation Tertiapin Degranulation of mast cells Procarmine (procamine A, B) Related to radiation protection Hyaluronidase Histolytic activity, antigenic component Phospholipase A ₂ (phospholipase A ₂₎ Destructive, Hemolytic, and Catalytic Activity of Cellular Tissues Alpha-glucosidase Antibody Role Enhancement Acid phosphomonoesterase Antibody Role Enhancement Lysophospholipase Inhibition of Phospholipase A ₂ Action Histamine Blood pressure strengthening, intestinal contraction Dopamine Neurotransmitter

The composition of each component in live bee venom and its action in mammalian in vivo so far are shown in Table 2 (Christopher MH. Kim, 1992).

As mentioned above, since the pharmacological treatment effect and immune function of live bee venom in mammals are superior to any other drugs, the present invention intends to enhance the immune function of piglets by injecting live bee venom. In addition, to improve the immunogenicity before the occurrence of the disease to prepare a method for preventing the disease.

On the other hand, the injection amount of live bee venom can be determined in the case of the extracted dry live bee venom, but it is difficult to accurately measure the injection amount of the live bee venom injected directly into the body tissue of the animal by live bees. Therefore, in the present invention, the dose of live bee venom is determined on the basis of the number of surgical bee venom based on the average live bee venom amount of one worker bee at least 15 days old is 0.03 mg.

According to Christopher MH. Kim (1992), the number of live bee venom injections for humans weighing 70 kg is limited to a maximum of 20 animals per day. It is said that bee venom can be injected. Animals have a shorter lifespan than humans and have a low resistance to various drugs, so they are considered to have a high therapeutic and preventive effect even if a small amount of live bee venom is injected.

For the treatment of live bee venom, it is necessary to first select and capture bee for live bee venom therapy. There are many varieties of honeybees from all over the world. The breeds of honeybees in our country are Asian (original) and Italian species, with most Italian species. Conventional honeybees can be used for the treatment, but the growing environmental conditions are more demanding than the Italian species, and their environmental adaptability is weakened, making them less popular. On the other hand, Italian species bees have high popularity, easy management, and good breeding ability, so that they can be used for farming in farms. In addition, it is highly adaptable to the domestic environment, and adapts well even if the breeding place is moved, and it is safe to manage due to its weakness. In addition, its properties are milder than conventional species, and its wintering ability is higher. Thus, in the present invention, live bee venom therapy is performed using Italian species bees.

There are three kinds of Italian bees: queen bee, drone, and worker bees. Queen bees have poison sac and bee sting, but there is only one in the group of rods. Worker bees continue to breed from spring to autumn, and the number of worker bees per group of beekeepers (single-phase 10) is approximately 20,000, and there are poisonous and bee stings that can be easily captured. .

Among the worker bees, there is a difference in the storage of live bee venom of the venom sacs. The bee for injecting live bee venom is preferably used to capture only mature worker bees 15 days or older after discharge. This is because mature bees 15 days of age or older after being released have strong bee stings and are well infused with live bee venom, and a large amount of live bee venom is injected. In worker bees over 15 days of age, about 0.02 to 0.04 mg (average 0.03 mg) of live bee venom is stored in the venom sac. Upon stimulation, bee venom is contracted by nerve action and bee is secreted through bee sting. After 17 days of age, the production of bee venom is almost stopped and the weight of the venom sac remains unchanged.

Among the worker bees for treatment, the external features of the bong are relatively large, with no hair and gloss in the head, chest and abdomen. The overall body color is dark black brown, and the nature is sensitive to external stimuli, so the attack is strong. The older the peak, the stronger the needle needle, the better tissue penetration and live bee venom injection.

For the treatment of live bee venom, worker bees must be caught at least 15 days of age. The lifespan of worker bees is not constant and varies depending on the season and the amount of labor. In the spring and autumn, the worker's activity reaches a maximum in the period of large milking, so it has a life span of 1 to 2 months, and in July to August in the cold season, it is a life span of 1 month as a metabolism promotion for temperature control. In winter, worker bees have low stamina and can survive for about 4 to 6 months.

When capturing surgical rods, special tweezers and portable surgical rods are needed. The tweezers use sharp and sharp tips and strong edges. In addition, the movable rod for the procedure is a small transparent plastic box of about 11 cm in width, 5 cm in height, and 6 cm in height, and all parts other than the bottom use an air window to allow air to pass through well. The moving rods can hold more than 250 Italian species of bees, and the bottom has partitions for food. When the bubble bee sugar is put into the partition, worker bees use it as food, so if food does not fall on the moving rod, worker bees can survive for about 10 to 20 days. The bee survives without supplying water in the moving rod.

Tips for capturing raw rods for treatment vary by season.

First, the worker bees may only be used in the season when worker bees are not active outside, i.e., from the late autumn to early spring, under the condition that the static state of the ball is not changed to dynamic within the peak as much as possible. Capture with tweezers. In more detail, a portion of the lid of the movable rod is gently opened, and when the live rod is captured, the leg part is picked up with tweezers and placed on the surgical rod.

Second, when there are not many bees that are active before the rumors of the worker bee, ie early morning from late spring to autumn, or just before sunset, approach quietly to the left or right around the front of the moving rod, and then use the prepared tweezers. Hold the leg of the patient and put it into the moving rod for care. Next, when worker bees go out through the rumors of moving rods (time of bee activity, April-November), bubble stool is fed into the moving rod for the procedure and the rubber band of the moving rod for the procedure is placed. After removing it for a while, open the door of the surgical rod for 1/2 to 3/4 and place it vertically on the rumor of the Bongun opened in slow motion. After 10 to 20 minutes in this state, about 50 to 300 worker bees enter the surgical rod without any impact on their own.

As mentioned above, the trapping of worker bees should always be done with tweezers. Worker bees can survive long periods of time without damage to the body (more than 20 days). If the abdomen, head, and chest are captured with tweezers, part of the body tissue is injured, so worker bees are killed within a short time, so it is difficult to perform live bee venom using worker bees.

Food is required to survive the worker bees captured into the surgical rod for a long time. If suitable food is added, bubble sugars are placed on the surgical rod, and the worker bee sucks this as a substitute for food, so the viability is extended. do. At this time, when liquid saccharide or complete saccharide is liquefied buried in the body of the bee cavities of the chest and abdomen of the bee is shortened the lifespan of the bee and will be dead because it is inactive.

On the other hand, since worker bees are exposed to temperatures below 5 ° C, their viability decreases due to low temperature shocks, and thus the moving rod should always be kept above 5 ° C. In particular, if you do raw bee venom injection at sub-zero temperatures in winter, warmth is essential. Worker bees caught on surgical moving rods during hot summer days should be stored in a cool place away from direct sunlight for long-term survival. In particular, put in hot direct sunlight or in the car interior in the summer is quick fatal. In addition, bees are very vulnerable to pesticides, so when using insecticides such as mosquito repellent or mosquito scent, they should be sequestered and disposed of at all times.

Always move the surgical rod into the air-protected dark protective net or paper bag not covered with vinyl, etc. to restrain the bee's activity when not in use to prolong its life.

The present invention is based on immunity enhancement based on body acupoints in pigs as published in heavy veterinary medicine (Jung Jung Il et al., 1992) and pig acupuncture treatment (Lee Jong-chang, 1979) for the purpose of immunization of piglets immediately after delivery. The bee sting blood level was selected to inject live bee venom.

<Example>

1 is a diagram showing the blood invasion for the immune promotion of newborn piglets according to a preferred embodiment of the present invention. According to a preferred embodiment of the present invention, a live bee venom therapy is performed on the basis of the incidence of bee venom to increase immunity as shown in FIG.

Hereinafter, with reference to Figure 1 will be described a live bee venom injection method for the immune boosting of newborn piglets.

In order to increase the immunity of new piglets, live bee venom is injected into the bee stings of pigs that are compatible with the immunization. To this end, first, a surgical rod for a procedure containing a raw rod is prepared, and the raw rod is captured one by one with tweezers from the prepared movable rod. In other words, open the door of the surgical rod containing the bee a little bit to capture the bee with tweezers, in this case pick up the body (chest or abdomen) of the bee. After capturing the live rods as described above, the live bee venom therapy is performed according to the procedure acupuncture points (= FIG. 1) for enhancing the immunity of the newborn piglets according to the preferred embodiment of the present invention.

In the stage of infusion of live bee venom, live bee venom is known to be haegyeol blood (10), glioblastic blood (12) and head blood (?? 口 穴) (14), Inject in. At this time, haemunyeol 10 is the left and right sides of the pig's navel center 1 ~ 2cm apart and the two head blood (14) is the pig's navel center. Haemunyeol (10) and head blood (14) is the blood that governs the digestive system of pigs. Point blood (12) is a recessed portion of the lower back of the anus, and the blood is responsible for the reproductive system and digestive system of pigs.

The number of injections of live bee venom is two live rods one by one on left and right of haemun blood (10), and graft blood (12) and head blood (14) are treated with one live rod, respectively. In this case, in order to rapidly spread live bee venom, it should be injected in the center of the bee sting blood. Live bee venom treatment of glioblastoma (12) is to increase resistance to natural exposure immediately after birth, and also to increase resistance to pathogenic microorganisms caused by various external factors. As a result, disease resistance to diseases such as piglet diarrhea increases, digestion is promoted, and the growth rate and feed efficiency of piglets are improved. Head blood (12) is to prevent the invasion of pathogenic microorganisms by cutting the umbilical cord of piglets immediately after birth to prevent pathogenic microorganism invasion, this procedure promotes wound healing of the umbilical cord cutting to prevent corditis.

On the other hand, the method of raw bee venom is selected or combined with the direct injection of live rods and abdominal cutting injection method.

The direct rod injection method is a procedure in which the raw rods directly removed from the moving rod for treatment are tipped directly into the affected part of the disease to be treated, and the needles of the needles remove only the body of the bee while it is attached to the body of the pig. Leaving the bee stings in body tissues causes the bee stings and the neural action attached to the live bee venom sacs to continue secreting the live bee venom into the tissues. Stinging needles embedded in the tissues will come out on their own after a certain period of time without being artificially removed.

Next, the abdominal amputation injection method removes the head and chest of the live rod and removes the needle to make it easier to perform with tweezers by grasping the live rod taken out of the moving rod with extra tweezers or the thumb and index finger of the other hand. Only the abdomen is isolated immediately before the procedure, and the needle is directly acupuncture by the abdominal contraction by the action of the abdominal contraction penetrates into the tissue, the abdomen is separated and the bee sting remains in the body tissue and the live bee venom is introduced into the body tissue.

If the immunity of newborn piglets is not enhanced even after the live bee venom therapy as described above, repeat the live bee venom therapy in the same way. This effectively enhances the immunity of newborn piglets.

Results and Discussion

(1) research background

The pharmacological action of bee venom has been shown to stimulate the immune system of mammals and have pharmacological effects such as anti-inflammatory and cell lysis, neurotoxin effect, antibacterial and antifungal action, protection from radiation and antialkylation.

In the immune system, no direct treatment of livestock disease by live bee venom has been found internationally. However, Cristopher MH (1992) is a preliminary experiment for the development of various diseases in humans. An example reveals the various mechanisms of bee venom.

The components of bee venom and bee venom have been developed in the body and the treatment method of various human diseases, showing high therapeutic effect and applying actual bee venom and bee needle therapy.

Professor Artemov (1959) of the Soviet Union said that bee venom stimulates the mammalian immune system to fight disease successfully. The main immunity of bee venom to the immune system is to stimulate the organism's biological system. It was said to increase.

Belleveau's team in the United States has demonstrated that bee venom has been shown to be effective in treating cancer cells in animals. However, bee venom itself has no direct effect on cancer cells or normal cells, but indirectly stimulates the immune system.

The injection of bee venom into the sheep resulted in the discovery of many immune spot-forming cells in the animal spleen immunized with red blood cells, which significantly affected the response of spleen cells to T-cell mesothelial substances. It suggests that it affects. In addition, bee venom has been shown to be effective for the function of T-cells and B-lymphocytes in the immune system of bee venom. The other action is an immune response that is thought to undergo antigen competition, and bee venom has the characteristics of suppressing the immune system. This phenomenon occurs in the components of bee venom in the molecular weight of less than 12,000-15,000 such as merittin (amin), apamin (apamin), mast cell degranulation peptide, proteinase inhibitors.

Phospholipase A ₂ , a high molecular weight component of bee venom, increases cell membrane permeability and hyaluronic acid enzymes promote antigen transport to immune-adapted cells.

In the preliminary experiments on livestock diseases, there is no report on the treatment of livestock diseases by injecting live bee venom in Korea, and our team tested the results of bacterial diarrhea of piglets in piglets, the absence of birth sows and mastitis. It was determined that the bee venom treated with bee venom had a pathogenic effect, and the humoral immunity was analyzed for the effects of live bee venom administration on immunity and tolerability enhancement.

(2) materials and methods

Public animals: Newborn piglets 1 day of age at the pig farm in Cheongju city, Chungcheongbuk-do were used as public animals. The test animals were divided into 32 piglets of 3 year old sows of 2-5 year olds, and 16 pigs were divided into 16 groups in the experimental group and 16 animals in the control group. Breeding with sows in the environment.

Bee Venom Procedure and Blood Collection: Control group was administered 0.2 ml of saline solution with 1 ml syringe to glioblastoma (upper anus, dent in the lower abdomen) and head blood (?? 口 穴). In the live bee venom treatment group, one Italian live rod was directly acupunctured in the ?? coccus, and one live rod was also acupunctured in the glioblastomas. The raw bee was used as a 15-day-old Italian honeybee ( Apis mellifera ) containing about 0.3mg of bee venom.

New piglets from the control and experimental groups were bled before and after 3 days, 1 week, 2 weeks and 3 weeks of treatment with saline and Italian rods. Blood collection is performed in the jugular vein after the new piglets have their limbs corrected by the supine position ^. Serum was automatically separated by gel & clot activator (Becton Dickinson Systems, USA) after collecting 3 ml with (Becton Dickinson Vacutainer Systems, USA).

Immunoassay: Serum from the jugular vein was naturally coagulated and the separated serum was stored in the refrigerator and measured for immunogloburin (Ig) G, IgA, and IgM using Cobas Integra (Roche, Switzerland) within 3 days by immunoturbidimetric method. It was.

Body weight and weight gain: The control and experimental groups were weighed and gained on digital scales before and after 3 days, 1 week, 2 weeks, and 3 weeks after drug administration.

Clinical findings of neonatal piglets treated with live bee venom: The clinical findings of newborn piglets treated with live bee venom increased slightly as the color of the pale body changed to pale peach as the blood circulation in the treatment area became more active. The live bee venom therapy of piglets rarely observed side effects such as allergy or shock reactions, which are sometimes seen in people with high blood pressure or heart disease.

Figure 1.Ig G changes in newborn piglets after live bee venom therapy

(4) strengthening immunity from live bee venom therapy

As shown in Figure 1 , Ig G showed a statistically significant difference compared to the control group administered with saline and head blood for three weeks after the bee venom treatment (p <0.01). As shown in Figure 2 below , Ig M tended to be slightly lower after 1 week of live bee venom administration, but no statistically significant difference was observed. (P <0.01). As shown in Figure 3 , Ig A also showed a higher tendency from 1 week after live bee venom administration. These results were consistent with Artemov's (1955) report on bee venom stimulating the immune system of mammals to protect against disease. Changes in these immune substances directly affected the weight and weight of newborn piglets.

Figure 2. Changes in Ig M in Neonatal Piglets after Raw Bee Venom Therapy

Figure 3. Changes in Ig A in Neonatal Piglets after Raw Bee Venom Therapy

Immunity enhancement of the newborn piglets according to the present invention as described above directly induces live bee venom to be injected by directly acupuncture the living bee stings in the bee sting place corresponding to enhancing the immunity of piglets immediately after birth. The treatment of live bee venom enhances the humoral immune substances in newborn piglets and increases resistance to the disease, thereby suppressing the occurrence of the disease.

Although described above with reference to a preferred embodiment of the present invention, those skilled in the art will be able to variously modify and change the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated.

Claims (4)

Preparing a moving rod for a procedure in which the raw rod is accommodated (S1); Capturing live rods by tweezers from the movable rod for the procedure (S2); And It is characterized in that it comprises a step (S3) is subjected to the captured live rods in the haengjeom blood (hae 門 穴), glioblastoma and head blood cells (?? 口 穴), which is a bee acupuncture point corresponding to the immunity enhancement of newborn piglets (S3) Immunity enhancement method of newborn piglets using live bee venom. The method of claim 1, wherein in the step (S3), by selecting or using one of live rod direct injection method or abdominal truncation injection method, a live rod is placed on the bee sting blood, and the immunity enhancement method of newborn piglets using live bee venom. The method of claim 2, wherein the direct rod injecting method is a method of directly acupuncture the live rods taken out of the surgical rod with tweezers on the needle acupuncture point, and the needles of the straight rods are embedded only in the body of the pig in the state of being embedded in the pig body tissue. The abdominal incision method is to remove the head and chest of the rod to make it easier for the tweezers to hold the back of the chest with the thumb and forefinger of the other hand of the operator's thumb and forefinger. Only the abdomen of raw bee with attached bee sting is separated immediately before the procedure, and the needle is placed directly on the bee sting blood. Immunity enhancement method of newborn piglets using live bee venom, characterized in that the injection. The method according to any one of claims 1 to 3, wherein the live bee is a mature worker bee at least 15 days after being released as an Italian honey bee.