KR20000032623A - Extract of natural substances for cosmetic - Google Patents

Abstract

PURPOSE: The extracts of natural substances for cosmetic are provided which is used as the raw material of cosmetic and obtained by the purification after heating and extracting natural substances. CONSTITUTION: The extracts of natural substances for cosmetics are obtained by the following steps. They are as follows: 1)The natural substances such as a delay seed coat, Lingusticum chuanxiong root, apricot stone, licorice, Scutellaria baicalensis, Angelica gigas root, mung bean, A gelicae dahuricae, Saururus chicness, ginseng leaf, Aconitum koreanum, Trichosanthis Radix, Eucommia ulmoides, Ledebouriella seseloides, Angelica tenuissima, Ampelopsis Radix, Cortinellus shiitake mushroom are mixed in the suitable mixing ratio, and 4-5 volumes of 1,3-butyleneglycol being 10% are added into the above raw materials. 2)The mixture is firstly heated at 65°C for 3-5 hours and then heated at 100-120°C for 3-5 hours again. 3)The mixture is extracted under the reduced pressure. 4)The extracts are filtrated using glass-filter-4G and con condensed under the reduced pressure. 5)The con condensed extracts are purified by column chromatography which is consisted of activated carbon and zeolite in a weight ratio of 3: 1 using water or ethanol as mobile phase.

Description

Extracts from natural products for use in cosmetics

The present invention relates to extracts for use in various cosmetics from natural products, and more particularly, to extracts extracted from natural products for use in raw materials of various cosmetics by filtering, separating and purifying by heating and extracting using natural products.

The skin surface of the human skin is sebaceous membrane, which is naturally mixed with sebum secreted from sebaceous glands and moisture from the glands, covering the stratum corneum to keep the skin smooth, shiny and moist.

The stratum corneum of the skin becomes the most ideal and beautiful state when the moisture is about 20%, and when the moisture of the skin falls below 10%, the skin becomes rough and the keratin comes off. Can not age.

However, humans are making great efforts to maintain beautiful and elastic skin, and spend much time and money on plastic surgery.

In addition, as a means to prevent aging and damage of the skin to compensate for by applying or edible to the skin using a variety of artificial composites, artificial composites can cause side effects such as rashes, allergies, etc. on the skin.

The present invention was devised to solve the above problems, the use of natural products is very toxic and adverse effects on the human body, to prevent peroxidation of the skin to the human body to increase the regeneration of the skin and to promote the metabolism of the skin The present invention relates to extracting an active ingredient from natural products that can inhibit aging of the skin by supplying and nourishing the skin.

1 shows the absorbency of the herbal extracts,

2 shows the epithelial cell proliferation effect of human,

3 shows antioxidant activity,

4 is a diagram showing tyrosinase activity inhibition.

Natural ingredients Ui-in, Cheongung, Apricot Seed, Licorice, Golden, Angelica, Mung Bean, White Paper, Triticale, Ginseng Leaf, Rich, Cheonhwabun, Yeonghyanghyang, Seongchung, Windproof, Gobon, Whiteworm, Shiitake Mushroom 10% of 1,3-butylene glycol (1,3-buthyleneglychol) (1,3BG / H20,10 / 90V / V) was added 4-5 times of the raw material and heated at a relatively low temperature for several hours before The extract was heated at a temperature for several hours, extracted under reduced pressure, concentrated by filtration, and concentrated under reduced pressure.

Hereinafter, the present invention will be described in detail.

Ui-in, Cheongung, Apricot Seed, Licorice, Golden, Angelica, Mung Bean, White Paper, Baiyun, Ginseng Leaf, Rich, Triticale, Cheonhwabun, Yeonghyanghyang, Tofu, Windproof, Gobon, Shiitake, etc. 30 ~ 40% by weight of the right weight ratio, mixed with Euiin, Cheongung, Angelica, Mung Bean, and Gold at the same weight ratio, and 20 ~ 30% by weight of the weight ratio of white paper, white worm, and natural flower 10% to 20% by weight, with the same weight ratio of windproof, hardwood, Youngyanghyang, and shiitake mushrooms, in the ratio of 5 to 10% by weight 5-10% made by mixing 85-10% of water with 5-10% of 1.3 butylene-glycol (1,3-buthylene glychol) in a volume ratio to the raw material containing% and other raw materials (apricot seed, rich) 1.3 butylene-glycol solution was added 4-5 times of natural raw material and heated at 65 ℃ for 3 ~ 5 hours. , And that the heat as the primary re-heating at 100~120 ℃ for 3-5 hours after hot water extraction under reduced pressure to give the glass-filtered in a filter paper -4 (glass-filter-4G), and then concentrated under reduced pressure to thereby.

The vacuum concentrated solution is separated and purified by eluting water or ethanol in a mobil phase by column chromatography in which activated carbon and zeolite are mixed in a ratio of 3 to 1 (weight ratio).

Referring to the components and the efficacy of each raw material to be combined to obtain the extract according to the present invention in detail as follows.

Euiin (薏苡仁) is a bark of the annual herb, cultivated in the field, and contains starch, protein, fat, carbohydrates, vitimin B _l , B ₂ , coixol, triterpenoids, and saponins. (Sapeonin) contains amino acids that make up protein, leucine, alanine, glutamic acid, arginine, tryptophan, asparagine, leucine is the main component of the protein.

Fats are in the form of glycerides such as palmitic acid, stearic acid, myristic acid, cis-8-octademic acid (coiccinic acid), and other β-sitosterols. Molecularly bound Coixenolide (C ₃₈ H ₇₀ O ₄ ) is contained, this component is effective in removing skin irritation or rough skin.

Cheongung is the root of the parsley and perennial Ligusticum chuanxiong Hort, which contains 1 to 2% by weight of essential oils. Acids (C ₁₂ H ₂₀ O ₃ ), butylphthalide, credium acid esters (C ₁₂ H ₉ O ₂ -OC ₁₀ H ₁₇ ), sedananoic acid (C ₁₂ H ₁₈ O ₃ ), and the like.

Cinidide is a granulant of cedanolide.

Cridilide (Kndium Lactone) Ligustilide

Credium

The main components of essential oils, clinilide, neocriidlide, and ligustilide, are intrinsic odors of the uterus and have mucosal congestion. Also, weak local irritation and small amounts excite the blood vessel motor nerves and respiratory organs and weaken the cerebrum. Numbness may indicate spontaneous weakness, sedation, respiration and excitement.

In addition, the extract has a sedative effect, it excites the central nervous system, lowers blood pressure, and functions to directly expand peripheral blood vessels.

Licorice is the root and root of licorice, a perennial herbaceous plant, and the roots and root stems are glycyricin (calcium salt of calicylic acid or calcirium salt) and glabraic acid (glycithin, C ₃₀ H). ₄₆ O ₅ ).

Glycidyl series acid (C ₄₂ H ₆₂ O _l6) is not loosened in the well pulrimyeo ether alcohol and hot water as triterpenoid saponins. After cooling in hot water, it hardens like grass.

Hydrolysis results in tasteless glycyrrhetinic acid (C ₃₀ H ₄₆ O ₄ ) and two molecules of glucuronic acid (C ₆ H ₁₀ O ₇ ).

Glycyretinic acid is a beta (β) amirin-based triterpenoid, and there are two kinds of alpha (α) and beta (β) bodies, which are easily changed.

However, their esters do not change with each other. Alpha (α) -glyciretinic acid is a plate crystal (dilute alcohol) and is soluble in ethanol, dioxane and chloroform.

Beta (gly) -glycyretinic acid is needle crystals (ethanol), soluble in ethanol, pyridine, acetic acid and less soluble in petroleum ether

In addition, flavanone glycosides, liqueurin (C ₂₁ H ₂₂ O ₉ ), neo-ricuritin, and rhamnoricuritin (all of which are non-glycosides are liquuritinin) and their corresponding galcon glycosides, isoricuiri Tin, neoisoricuriritin, isoram noricuriritin, and liqueuride (all of which are non-sugars are isosuricurigeninin).

Rhamnoricuritin seems to be called liqueuridoxide.

More than 27 flavonoid-like substances have been identified by chromatography in licorice roots.

Glycyricin

Glybraic Acid Glycyretinic Acid

Liqueurin liqueur seedine

In the licorice root, beta (β) -sitosterol, formmononetin, ricolycidin lycoconone and lyconoligonan were identified.

Flavonoid content in licorice changes with the season of growth.

Stems and leaves include saponaretin, glyphoside and bitexin.

Saponaretin is about 50% by weight of the total flavonoids and about 3% by weight in the outpost when the fruit is opened.

In addition, the bitter taste of gritillamarine (the bitter component of gritrizine), 2,4,4-trihydroxygalcon and its glycosides deoxystiggmasterin, beta (β) -sitosterin, essential oils, ascorbic acid , Asparagine, fructose, glucose, mannirol, starch, gum, yellow cow, malic acid, oil, resin, tannin, protein, amino acid asparagine, proline, alanine, threonine, serine, glycine, valine, isoleucine, gamma (γ) -Aminobutyric acid.

Glycylysine is not toxic, has anti-inflammatory action, inhibits the reductive action of △ ⁴ -3 ketones, which is an inactive process in the body of corticosteroids, and has a cortical hormone-like acting action, and against posuchlortal, strychitin, cocaine, etc. Relieves addiction, antagonizes with acetylcholine, histamine, allergens and anti-inflammatory.

In addition, glycyric acid has a strong surface activity, so when mixed with other pharmacological agents, the constituents of the medicinal herb can be loosened in water, help absorption, and reduce the toxicity to change the taste. Use a lot.

Danggui (는) is the root of the female donkey, which does not come out of the perennial Angelica, in the hot water, and dried. The components are essential oil and coumarin, and coumarin is decursin (C ₁₉ H ₂₀ O ₅ ) and decursinol ( C ₁₄ H ₁₄ O ₄ ), pyranokumarin, and nodakenetine (C ₁₄ H ₁₄ O ₄ ), umbeliferon, and nodakenin. Others include xanthotoxin, isopimpineline, ostol, ombelif There is an ingredient in the terrin.

Umbelliferon

Essential oils are composed of ligster, N (butyl) phthalide, O (O) -walerophenone carboxylic acid, water soluble alkaloid, vitamin B2elb (B ₁₂ ), anti-inflammatory effect, liver protection and glycogen prevent the reduction of (glycogen) and acts against the vitamin cucurbit elbeu (B ₁₂₎ deficiency.

In addition, it is used in gynecological disorders, menstrual irregularities, essential oils other than uterine contractions and essential oils at the same time the uterine relaxation.

Mung beans are used as seeds per year, and the main ingredient contains vitamins, carbohydrates, proteins, and fats, and the vitamins are provitamin A (A), vitamin bee (B ₁ ), beet (B ₂ ), and bithumus (B ₃ ) There is.

Carbohydrates include starch, pentozan, calactan and glucose, raffinose, and dextrins. Proteins include pazeolin.

In addition, there are nitrogen compounds such as paseoosaponin C ₅₀ H ₈₄ O ₂ , adenine, choline, hypoxanthine, arginine, aspartic acid, glutamic acid, lysine, florin, leucine, isoleucine, valine, and alanine.

In the treatment of motion, the seeds are used alone, in severe intoxication, alcohol poisoning and fever lowering medicine, and in particular, the skin is clean.

Gold is the root of perennial thimble with Lamiaceae, Baikalin (C ₂₁ H ₁₈ O ₁₁ ), Baikallein (C ₁₅ H ₁₀ O ₅ ), Wigonin (C ₁₆ H ₁₂ O ₅ ), Wargonoid ( C ₂₂ H ₂₀ O ₁₁ .4H ₂ O), 7-methoxy-bicaine (C ₂₂ H ₂₀ O ₁₁ ), neobicalane (C ₁₉ H ₁₈ O ₈ ), 7-methoxynorwegonin (C ₁₆ H ₁₂ O ₅ ), flavonoids such as aurocillin A (C ₁₆ H ₁₂ O ₅ ), school cap flavone I (C ₁₇ H ₁₄ O ₆ ) and school cap flavone II (C ₁₉ H ₁₈ O ₈ ), In addition, there are pyrocatelkin tannins, beta (β) -stosterol, and resin essential oils.

In addition, wogonoside (wargo-7-glucuronide), cartamidine, and isocartamidine are contained.

Baikal Baikal Lane Hugonoside

Flavonoids can be added to water, boiled, and then extracted from boiling water. The various flavonoids have excellent anti-inflammatory, antimicrobial and antioxidant activity. Baikallein inhibits capillary permeability and Baikalin does not inhibit histamine skin reactions. It inhibits anaphylaxis, but Baikalin inhibits both histamine skin reactions and anaphylaxis.

Baikalin has an antiallergic effect and maintains skin elasticity by inhibiting antihistamine activity and degranulation of mast cells.

Other components include tannins, beta (β) -sitosterol and essential oils.

Baekji (白芷) is the root of perennial herbaceous plants of the family Apiaceae contains a large amount of coumarin, among which Baengangelin (C ₁₇ H ₁₈ O ₇ · H ₂ O), Baeng Angelico (C ₁₇ H ₁₆ O ₆ ), Imperatorin (C ₁₆ H ₁₄ O ₄ ), xanthoroxine (C ₁₂ H ₈ O ₄ ), pelophterin (C ₁₇ H ₁₄ O ₅ ), etc., and the structural formula of coumarin

In case of CHOH-C (CH ₃ ) ₂ OH instead of R is white angelicin,

CH-C (CH ₃ ) ₂ , instead of R ₁ , is white angelic glycol,

If CH = C (CH ₃ ) ₂ instead of R, it is ferrophrine.

Imperatorine Xanthoroxine

It stimulates the center, enhances breathing and increases blood pressure.

It is a vine growing perennial grass and contains a large amount of mucus. It is named in the sense of treating salt sores. It has excellent anti-inflammatory and skin regeneration, excellent analgesic and moisturizing properties.

Cranberries are dried perennial roots of sky watermelon and are called cheonhwa because the powder of the root is white like flour. It contains ingredients of starch, stigmasterol, beta (β) -sitosterol, saponin, and basic protein tricosanthin.

Trichoxanthine, an organic acid, has the effect of abortion, and is used for fever-lowering medicine and salt medicine, and supplements nutrition when there is a lot of sweating, urination, and body fluids caused by inflammation or fever.

In addition, it promotes diuretic action and induces excessive skin excretion by hypersecretion of the sweat glands into the excretion to the kidneys.

There is an effect as described above is effective for purulent diseases.

Windproof (防風) is the root of the perennial herbaceous plant of Apiaceae, contains essential oil and coumarin, and inhibits some skin fungi.

Gobon is a perennial herb that contains furokmarin and essential oils. The composition is still unknown and is effective in treating skin diseases.

Shiitake mushroom is a kind of mushroom that emerges from dead oak, chestnut, oak, zelkova, mulberry, etc. It contains crude protein, crude fat, crude fiber soluble nitrogen-free substance, ash, etc. It also contains polysaccharides that are well soluble in water.

There is an anti-active component, thionionine (C ₂ H ₄ S ₅ ), lentinane is a kind of polysaccharides have an immuno-active activity.

Ginseng is a perennial herbaceous plant that uses all roots and leaves, but usually uses many roots, and contains glycosides, essential oils, oils, amino acids, alkaloids, etc. Glycosides are divided into what are called ginsenoids and panaxoxide seeds. .

However, all have protopananaxol, protopananaxtriol, oleanoic acid as genine and glucose, rhamnose, arabinose as binding sugar.

The panaxoxide is composed of panaxoxide A (glucose 3 molecules), which contains protopanaxanadiol as a genine, panaxocside B (2 molecules of glucose and 1 molecule of rhamnose), and panaxoxide C (3 molecules of glucose and 1 molecule of rhamnose). ), And Panaxoside D (four molecules of grapefruit) with Genin as Protopanaxanatriol, Panaxodide E (four molecules of glucose and one molecule of arabinose), and Panaxanthide F (six molecules of glucose).

Ginsenoids are ginsenoids with 20- (S) -protopanaxadiol as genine Rb ₁ (1 molecule of glucose), Rb ₂ (3 molecules of glucose and 1 molecule of arabinopyraz), Rb ₃ (3 molecules of glucose and xyl) 1 molecule of Rose), Rc (3 glucose and 1 arabinopranose), Rd (3 glucose) and Re (2 molecules of glucose, Rhamnose 1 with 20- (S) -protopanaxatriol) Molecules), Rf (two glucose molecules), Rg ₁ (VHEHEKD 2QNSWK), Rg ₂ (one molecule glucose, one molecule of rhamnose), and ginsenoside Ro (two molecules glucose and one glucuronic acid 1) Molecules).

Ginsenoside Ro Panaxtrium panaxadiol

Essential oils are terpene compounds with the smell and bitter taste of ginseng, called panaxen, and the components are beta (β) -elemen (C ₁₅ H ₂₄ ) and falcarinol (CH ₂ = CH-CHOH- (C≡C)). ₂ -CH ₂ -CH = CH- (CH ₂ ) ₆ -CH ₃ ), and palicanol has an unstable property that is easily resinized in air.

Amino acid components include choline, arginine, glutamic acid, chisteine, tyrosine, and α-aminobutyric acid.

In addition, Panaxide F contains panaxoxide F, which is a unique ingredient found only in outposts, and also glycerin, astagallin (camperol-3-glucoside), and glycosides of quergestin, tripolin, and panaceno. Seed (camperol-3-0-glucogalactosid), luteolin-7-glucoside, nonacoic acid, octacosanol, clioastenol.

20 (S) -protopanaxatriol

R ₁ = R ₃ = H, R ₂ = OH

The larvae are perennial trees and the components are chlorogenic acid C ₁₆ H ₁₈ O ₉ , caffeic acid, acuvin (linanthin or aquaboside) C ₁₅ H ₂₂ O ₉ (alcohol and water, soluble in ether, acetone and benzol) Not rubbery).

Further, glycosides include logannin C ₁₇ H ₂₆ O ₁₀ (unsolved in water, alcohol, ether, chloroform).

In addition, there are herpagide acetate C ₂₆ H ₃₂ O ₁₂ , eucommiol C ₉ H ₁₆ O ₄ , ayugoside, lephthoside, flavonoids, alkaloids, organic acids, resins, vitamin C and the like.

Bark, branches, leaves and roots are rubbery.

This rubber is not elastic rubber (cow chuk), but Gooda Perca (C ₅ H ₈ ) n has a molecular weight of 1.4 × 1.8 × 10 ⁵ .

In addition, the skin contains organic acid, chlorogenic acid and vitamin C, and a small amount of alkaloid resin.

Aucuvin Loganin

Chlorogenic Acid Caffeic Acid

Tofu with the above components has the effect of stopping the tonic, gangjeong, soothing, pain, in particular, the effect of reducing the hull pressure by inhibiting the exercise center and vagus nerve of the soft water.

Apricot seeds, also known as passersby, are mature seeds of the apricot tree, a deciduous arborescent plant, skinned, ground, peeled, and dried only in soybean seeds.The ingredient is an amygdalin (C ₂₀ H ₂₇ O ₁₁ N) enzyme. Contains olein, the main component of phosphorus mulsin, protein and oil.

Amigdalin is hydrolyzed by a foreign body, amidalase, prunase, oxynitdalase.

Amigdalaze

C ₆ H ₅ -CH-OC ₆ H ₁₀ -O ₄ -OC ₆ H ₁₁ O ₅ → C ₆ H ₅ -CH

Amigdaline + H ₂ O Prunasin

Prunase

+ C ₆ H ₁₂ O ₆ Prunasine + H ₂ O → C ₆ H ₅ CH (OH) CN + C ₆ H ₁₂ O ₆

Glucose Mandelonitrile Glucose

Oxynitrilease

→ C ₆ H ₅ CHO + HCN

Benzaldehyde Hydrogen Cyanide

The fruit flesh contains sugars, dextrins, malic acid, and organic acids such as lemon acid, and quercithin, isocercithin, gamma (γ) -aminobutyric acid, vitamins A and B ₂ , catechol and various sterols. Contains various fats.

The trace amount of cyanic acid produced after the hydrolysis of the amigdaline has a calming action to calm the respiratory center and restore the normal respiratory movement to normal.

In addition, amigdaline is known to have anticancer activity and may be used as a cancer treatment agent.

That is, since cancer cells have higher concentrations of beta (β) -glucuronidase than normal cells, they rapidly decompose amidaline and accumulate cyanic acid at a higher concentration.

This cyanic acid inhibits important cytochrome oxidase in respiratory cells.

In addition, cyanate ions are highly poisonous to cancer cells because cancer cells have low concentrations of rodanase, an enzyme that protects cells from cyanic acid poisoning.

Eventually, amidaline selectively inhibits cancer cells and causes them to die.

Three hundred seconds is a perennial grass used for swelling.

It is a processed product of the root of Odu, a rich perennial herbaceous plant. It is a young root of the perennial herbaceous plant, and it is highly toxic.

To remove the poison, repeat dipping several times in a mixture of salt (40% by weight) and crust (60% by weight) for a long time, or use it as a medicine if there is no tongue numbness by steaming the soak in the mixture of salt and crust. .

The components are diderpene alkaloids and alkaloids can be divided into two categories.

Arcotin-based alkaloids contain a methoxy group and a hydroxyl group, and have two aroxyl groups (R-CO-CO), which belong to this group of aconitine (C ₃₄ H ₄₇ O ₁₁ N) and mesaconitin (C ₃₃ H ₄₅ O ₁₁ N), hypoaconitin (C ₃₃ H ₄₅ O ₁₀ N), esaconitin, thalatisamine (C ₂₄ H ₃₉ O ₅ N), cheondual alkaloid A (C ₂₃ H ₃₇ O ₆ N), thioalkaloid B (C ₂₂ H ₃₅ O ₄ N) and the like, there is an amino alcohol alkaloid without ester bonds or methoxy groups in the molecule, and the components belonging to it such as artisin, napelin, ignabin, Hippognabin and Kobusin.

In particular, other components having cardiovascular activity include hygenyamin, corynechloride, and yokonoside.

Higenyamin corynein chloride

The highly toxic aconitine-based alkaloids are easily processed into diester acyl groups, which are monoester type, and further processed into amino alcohols.

That is, aconitine → benzoyl aconine → aconine, mesaconitin → benzoyl mesacinine → mesacinine, hypoaconitine → benzoyl hypopoonin → hypoaconine, esaconitin → benzoyl esacinine → it is broken down into esaconia.

In other words, the processed molecules are low in alcoholoid content and high in monoester type or amino alcohol with low toxicity.

As shown in Table 1 below, the alkaloid content, particularly the diester alkaloid, is less when processed, and the monoester type is not much different between the processed and the unprocessed.

[Table 1] Alkaloid Content of Rich and Processed Products

Alcoholoids, which are monoesters, are less toxic and pharmacological, promoting the metabolism of saturated fatty acids and cholesterol, and reducing them in the artery wall and blood.

<Example 1>

Euiin, Cheongung, Angelica, Mung Bean, Golden: 40% by weight,

Three hundred seconds, white paper, baekryeom, cheonhwahwa mixed at the same weight ratio: 25% by weight,

Windproof, Gobon, Yeongnyanghyang, Shiitake mushrooms: 20% by weight,

Panax ginseng leaf, mixed with the same weight ratio: 10% by weight,

Apricot seeds, three hundred seconds, rich: 5% by weight of the same amount of the mixed ratio of 5% by weight of the blended ingredients, 10% of 1.3-BGG (1.3-Buthyleneglychol) is added to the blended material about 5 times the raw material volume, 60 After heating for 4 hours at ℃ and again heating for 4 hours at 110 ℃ and then extracted with boiling water under reduced pressure and filtered in glass-filter-4G (glass-filter-4G) and concentrated under reduced pressure to make a reduced pressure concentrated liquid, The zeolites were separated and purified by eluting into a fluidized bed by column chromatography mixed 3 to 1 (weight ratios). This solution was called an extract extracted from natural products.

This extract is added to a formulation that makes up basic cosmetics (lotion, nourishing cream, skin, essence, etc.) to make basic cosmetics by adding 0.5 to 3% by weight to the skin and human skin for stability and efficacy tests. Was carried out.

<result>

The general characteristics of the separated tablets were PH 6.4, slightly acidic, and the viscosity was brownish mucus.

The extraction efficiency as a solid content was 38 weight%.

The tyrosinase inhibitory activity of the extracted extract was as shown in Table 2, and the extracted raw material was added to the formulation of the basic cosmetics (lotion, nutrition cream, skin, essence, etc.) by adding 0.5 to 3% by weight After making a thick coat on the rabbit's ear canal, the rash test was performed.

When applied to the skin of a person (200 people) and conducted a clinical experiment, as shown in Figure 1 showed a very excellent moisturizing, as shown in Figure 2 to reinforce the skin elasticity to eliminate fine wrinkles, Figure 3 As shown in Table 2, very effective results in skin aging inhibition were obtained.

[Table 2] Tyrosinase Inhibitory Activity of Extracts

<Example 2>

Basic cosmetics of the cleansing system were prepared using the extract extracted by the extraction method of <Example 1>.

The extract of <Example 1> was added to the base cleaning system (form cleansing, cleansing cream, pack) 2 to 6% by weight to make a product.

<result>

The product was thickly applied to the rabbit (10) ear canal and tested for rash.

As a result of clinical trials applied to the skin of people (200 people), as shown in FIG. 1 without repercussions, very excellent moisturizing and skin elasticity, and as shown in Figure 4 melanoidin pigmentation markedly Suppressed.

It also moisturizes the skin.

Extracts having the above ingredients can also be used in various skin cosmetics such as cosmetics and soap, and can be used in various industrial products, including various detergents and leather products, skin-related products.

In other words, in Figure 1, the results of experiments on the absorbency of the herbal extracts showed the most excellent baekryeom and the composite extract used in this product also showed excellent absorbency.

In order to enhance the elasticity of the skin and examine the proliferation of the cells by adding various extracts to human epithelial cells in order to see the regeneration effect of the skin, as shown in Figure 2, both extracts showed an effect on cell proliferation, especially the complex extracts Was outstanding.

Figure 3 is to examine the antioxidant effect of the various extracts were the best gold and three hundred seconds and the complex extract also showed a high antioxidant power.

Figure 4 is a measure of the activity of tyrosinase of various extracts, which is used as an indicator for confirming whether the skin blemishes. As shown in FIG. 4, baekbaekpi was the most excellent and the complex extract also showed an excellent effect on the inhibition of tyrosinase activity.

Extracted and purified from natural products as described above has the following effects.

Although it does not seep into the skin, it forms a protective film on the skin to help keep the texture of the skin soft.

Various flavonoids of gold and Rb ₁ in ginseng leaves, various vegetable sterols and beta-carotene prevent skin aging.

Skin soiled with cosmetics and pollutants is cleaned by mung bean ingredients that cleanse, and it has excellent antibacterial and anti-inflammatory properties such as acetylcichoriin, and trichosanoic acid contained in natural powder. Because it contains, it removes contaminants from the surface of the skin and pores to keep the skin clean and at the same time protects the skin and whitening effect.

Licorice contains hygenamine, retinan and glycyrrhizin, which promote skin metabolism and enhance skin regeneration, contributing to the activation of skin.

The mucus contained in baekryeom enhances the skin's moisturizing power to maintain the elasticity of the skin.

The above effects are synergistic effect of the components contained in each of them, but also interact with each other.

Claims (1)

30 to 40% by weight of the mixture of Euiin, Cheongung, Angelica, Mung Bean, and Gold at the same weight ratio, and 20 to 30% by weight of the mixture of three hundred seconds, white paper, baekryebi and cheonhwa in the same weight ratio, windproof, 10 to 20% by weight of Gobon, Yeonghyanghyang and Shiitake mushrooms at the same weight ratio, 5 to 10% by weight of parchment paper, white and ginseng leaves at the same weight ratio, apricot seed and rich 3 to 5% by weight of the mixture in the same weight ratio, respectively, 10% of 1.3 butylene-glycol (1,3-buthylene glychol) (1,3BG / H20,10 / 90V / V) Four to five times the amount of the raw material was firstly heated at 60 to 70 ° C. for 3 to 5 hours, and then the first heated was heated at 100 to 120 ° C. for 3 to 5 hours to extract hot water under reduced pressure. Was filtered through glass-filter-4G, and then concentrated under reduced pressure to obtain a concentrated liquid. In natural products for use in cosmetics characterized in that the zeolite is separated and purified by eluting water or ethanol in a mobil phase by column chromatography mixed in a ratio of 3 to 1 (by weight). Extract to extract.