KR100372359B1 - Cosmetic Composition Containing Lentinus Edodes Sing. Extracts or Paceilomyces japonica Extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition, the cell growth promoting component, Cordyceps sinensis extract; And / or oligo lentinan obtained by acid treatment of lentinan extracted from shiitake mushrooms.

Description

Cosmetic composition containing shiitake mushroom extract and / or Cordyceps sinensis extract [Cosmetic Composition Containing Lentinus Edodes Sing. Extracts or Paceilomyces japonica Extracts}

The present invention relates to a cosmetic composition containing shiitake mushroom extract and / or Cordyceps sinensis extract, and more specifically, after obtaining a high-volume natural polysaccharide lentinan from shiitake mushroom (Lentinus Edodes Sing) through the chemical treatment and skin absorption ability It relates to a composition of a cosmetic having a skin cell proliferative effect, characterized in that it comprises an extract obtained by separating and purifying oligo-lentinan of high molecular weight, and extracts extracted from Cordyceps sinensis ( Paceilomyces japonica ), respectively or together.

Skin is an important tissue that functions to protect the body from the external environment biochemically and physically. The skin is largely epidermis composed of keratinocytes and melanocytes, dermis composed of collagen fibers and elastin fibers, and the dermis. hypodermis).

The skin ages more easily than other tissues of the human body due to various environmental conditions such as ultraviolet rays, smoking, stress, disease, and exposure to pollutants. Over time, wrinkles are formed due to internal and external structural changes, shine and flexibility. You lose your back.

On the other hand, the skin is composed of several kinds of binding proteins and cells, the regeneration and proliferation of these skin cells plays an important role in the composition of the binding protein, accordingly, the growth and proliferation capacity of the skin cells is a skin It is the most important factor directly related to delaying aging. In view of this, various attempts have been made to extract natural substances having skin cell proliferation effects and add them to cosmetics, and mushrooms are one of the objects.

Korean Patent Laid-Open Publication No. 1996-69542 discloses a cosmetic composition containing a situation mushroom extract, but a situation mushroom extract was characterized by a whitening effect, not a skin cell proliferation effect.

Accordingly, it is an object of the present invention to provide a natural extract excellent in skin cell proliferation effect.

Another object of the present invention is to provide a natural extract that is well absorbed by the skin.

Another object of the present invention to provide a cosmetic composition having a skin cell proliferation efficacy.

1 is a graph showing the cell proliferation effect according to the concentration of Cordyceps sinensis extract.

Figure 2 is a graph showing the cell proliferation effect according to the concentration of oligo-lentinan of shiitake mushrooms.

Figure 3 is a graph showing the cell proliferation effect according to the concentration of Cordyceps Sinensis and Oligo-lentinan mixture.

Figure 4 is a graph showing the skin elasticity improvement effect of the cosmetic containing Cordyceps sinensis and Oligo-lentinan mixture.

An object of the present invention relates to a cosmetic composition, as a cell growth promoting component, Cordyceps sinensis extract; And / or oligo lentinane obtained by acid treatment of lentinan extracted from shiitake mushrooms.

The inventors of the present invention searched for various medicinal plants and mushrooms in order to find a substance having excellent skin cell proliferation effect, and found that shiitake mushroom and Cordyceps sinensis extract have a great effect.

Cordyceps sinensis is a type of asymptomatic fungus in which fruiting bodies (mushrooms) are formed. Cordyceps sinensis invades insects and forms fruiting bodies on the larvae based on them. Some species of Cordyceps have been used since ancient times in China as antidote for tuberculosis, asthma, jaundice and antidote for opioid poisoning, post-mortem and tonic, and immune enhancers. It is an expensive herbal medicine [Reference (1). Humber, R. A. 1990. Fungal pathogens of insects, spiders, and mites; isolation, preservation, and identification. USDA Agricultural Research Service; Reference (2). Jianzhe, Y., Xiaoloan, M., Qiming, M., Yichen, Z. and Huaan, W. 1989. Icons of medicinal fungi from China. Science Press. China. p. 575; Reference (3). Kobayasi, Y. 1940.The genus and its allies. Sci. Rept. Tokyo Bunrika Daikaku, Sect. B., 5: 53-260; Reference (4). Shimizu, D. 1994. Color iconography of vegetable wasps and plant worms. Seibundo Shinkosha. Japan. pp. 381]. When analyzing the components of Cordyceps sinensis, 11 kinds of amino acids necessary for the human body and 10 kinds of amino acids with medicinal efficacy account for more than 60% of the total content, respectively, and cordycepic, an isomer of quinic acid. acid) about 7%. Also, linolenic acid, an antioxidant lipid required for cell activation, and 3'-deoxyadenosine, a nucleic acid, are included. In addition, various pharmacological effects are known, such as immune enhancement. [Cunningham, K. G., W. Manson. F. S. Spring and S. A. Hutchinson. 1950. Cordycepin, a metabolic product from cultures of (Linn.) Link. nature. 166. 949]

The main component of shiitake mushroom is lentinan, β-glucan, a natural polysaccharide. Lentinane is a natural polysaccharide component that helps cell proliferation, immune boosting and cell activation. However, when applied to the skin there is a disadvantage that the absorption is not due to the high molecular weight. In the present invention, a low molecular weight oligo-lentinane is prepared through chemical treatment in order to increase the skin absorbency of lentinan and then added to the cosmetic. Extraction method of Cordyceps sinensis extract is as follows.

After completely drying the Cordyceps sinensis, one or more solvents selected from the group consisting of water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, ethylene glycol and propylene glycol are added to the completely dried Cordyceps sinensis. 10 to 25 times by volume, and extracted by heating at 50 to 100 ° C. for 3 to 20 hours in a state in which evaporation of the solvent is prevented by a cooling condenser or dipping at 5 to 37 ° C. for 1 to 15 days. The extract is concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser to recover the evaporated solvent. Cordyceps sinensis extract is added to the cosmetic in an amount of 0.001-5% by weight, preferably 0.001-0.5% by weight. When Cordyceps sinensis extract is added to cosmetics, it is dissolved in an alcohol such as ethanol or a glycol such as butylene glycol or propylene glycol.

Method for preparing oligo lentin from shiitake mushrooms of the present invention is as follows.

After drying the shiitake mushrooms, a solvent selected from the group consisting of water, phosphate buffer solution, acetic acid buffer solution, weakly basic aqueous solution, glycerin, ethylene glycol and propylene glycol was added in a volume of 5-30 times to the dried mushrooms. In the state in which evaporation of the solvent is prevented by the cooling condenser, extraction is performed by heating at 60 to 100 ° C. for 4 to 48 hours or by depositing at 5 to 37 ° C. for 1 to 15 days to extract the active ingredient. The extract is precipitated in one or more solvents selected from the group consisting of ethanol, methanol, propanol, butanol, acetone, benzene, ethyl acetate, acetonitrile, tetrahydrofuran, and the precipitate is filtered and dried to obtain lentinan solids. The solid is added to an acid such as dilute hydrochloric acid, dilute sulfuric acid, dilute nitric acid, formic acid, acetic acid, etc., heated in a reactor with a cooling condenser for 1 to 10 hours at 60 to 100 ° C, filtered and concentrated under reduced pressure to obtain oligo-lene. Get Tinan Solids. The oligo lentinane solids are added to the cosmetics in an amount of 0.001-5.0 wt%, preferably 0.001-0.5 wt% by dry weight thereof, and the oligo lentinane solids are added to the cosmetic in the form of an aqueous solution dissolved in purified water.

Cordyceps sinensis extract and / or oligo lentinan of the present invention are added to cosmetic formulations such as lotion, nutrition lotion, nutrition cream, massage cream, nutrition essence, emulsified foundation.

The present invention will be described in detail with reference to the following Examples.

Preparation of Cordyceps Sinensis Extract

Example 1

1 kg of Cordyceps sinensis was thoroughly dried by washing with purified water in 20 kg of water, extracted by heating in an extractor equipped with a cooling condenser for 5 hours, filtered through a 400 mesh filter cloth, cooled at room temperature, and left to stand at 5-15 ° C. for 7 days. After filtration was performed using Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 26 g (dry weight) Cordyceps sinensis extract.

Example 2

1 kg of Cordyceps sinensis thoroughly washed with purified water in 20 kg of 50% ethanol, extracted by heating in an extractor equipped with a cooling condenser for 5 hours, filtered with 400 mesh filter cloth, cooled at room temperature and left at 5-15 ° C. for 7 days. After aging, the filter was filtered using Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 25 g (dry weight) Cordyceps sinensis extract.

Example 3

1 kg of Cordyceps sinensis was washed with purified water and dried in 20 kg of 70% ethanol, and extracted by heating in an extractor equipped with a cooling condenser for 5 hours, filtered through a 400 mesh filter cloth, cooled at room temperature, and left at 5-15 ° C. for 7 days. After aging, the filter was filtered using Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 20 g (dry weight) Cordyceps sinensis extract.

Example 4

1 kg of Cordyceps sinensis was washed with purified water and dried in 20 kg of 70% ethanol, and extracted by heating in an extractor equipped with a cooling condenser for 5 hours, filtered through a 400 mesh filter cloth, cooled at room temperature, and left at 5-15 ° C. for 7 days. After aging, the filter was filtered using Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 20 g (dry weight) Cordyceps sinensis extract.

Example 5

1 kg of Cordyceps sinensis thoroughly washed with purified water in 20 kg of ethanol and extracted for 7 days at 15-37 ° C., filtered through a 400 mesh filter cloth, cooled at room temperature, left at 5-15 ° C. for 7 days, and then aged. It was filtered with filter paper twice. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 20 g (dry weight) Cordyceps sinensis extract.

Example 6-21

Using the solvent of Table 1 below and extracting in the same manner as in Example 5 and the results are shown in Table 1 below.

Table 1

Example No. Dry Weight of Extraction Solvent Final Extraction (Unit: g)

Example 6 50% Ethanol 25

Example 7 80% Ethanol 19

Example 8 Methanol 19

Example 9 50% Methanol 21

Example 10 80% Methanol 23

Example 11 n-propanol 17

Example 12 Isopropanol 18

Example 13 n-butanol 16

Example 14 Functional Glycerin 24

Example 15 Hydrated Propylene Glycol 23

Example 16 Hydrated Butyleneglycol 23

Example 17 Benzene 12

Example 18 Chloroform 11

Example 19 Hexane 8

Example 20 Acetone 13

Example 21 Purified Water 27

Preparation of Oligo Lentinan

Example 22

1 kg of shiitake mushrooms, which were washed with purified water and dried completely, were placed in 16 kg of water, and the pH was adjusted to 10 using an aqueous sodium carbonate solution, followed by extraction at 100 ° C. for 8 hours. The extract was centrifuged to give a supernatant, adjusted to pH 4, precipitated in 12 kg of ethanol, filtered and dried to give 50 g of lentinan. 50 g of lentinane was added to 2 kg of formic acid, heated in a reactor equipped with a cooling condenser, heated at 100 ° C. for 3 hours, filtered through a 400 mesh filter cloth, concentrated under reduced pressure, dissolved in purified water, filtered through a deodorized 0.45 μm filter, and a 1 kg aqueous solution was obtained. .

Example 23

1 kg of shiitake mushrooms washed with purified water and dried completely were put in 16 kg of a phosphate buffer solution at pH 10, and extracted at 100 ° C. for 8 hours using an extractor. The extract was centrifuged, the supernatant was taken, precipitated in 12 kg of ethanol, filtered and dried to give 45 g of lentinan. 45 g of lentinane was added to 2 kg of formic acid, heated at 100 ° C. for 3 hours in a reactor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, concentrated under reduced pressure, dissolved in purified water, and filtered through a deodorized 0.45 μm filter to obtain an aqueous solution of 0.9 kg. .

Example 24

1 kg of shiitake mushrooms, washed with purified water and dried completely, were placed in 16 kg of a phosphate buffer solution at pH 7.4, followed by extraction at 100 ° C. for 8 hours. The extract was centrifuged, the supernatant was collected, precipitated in 12 kg of ethanol, filtered and dried to obtain 40 g of lentinan. 40 g of lentinane was added to 2 kg of formic acid, heated in a reactor equipped with a cooling condenser, heated at 100 ° C. for 3 hours, filtered through a 400 mesh filter cloth, concentrated under reduced pressure, dissolved in purified water, and filtered through a deodorized 0.45 μm filter to obtain an aqueous solution of 0.8 kg. .

Example 25

1 kg of shiitake mushrooms, which were washed with purified water and dried completely, were placed in 16 kg of water, and the pH was adjusted to 10 using an aqueous sodium carbonate solution, followed by extraction at 100 ° C. for 8 hours. The extract was centrifuged to give a supernatant, adjusted to pH 4, precipitated in 12 kg of ethanol, filtered and dried to give 50 g of lentinan. 50 g of lentinane was added to 2 kg of 2N hydrochloric acid, heated at 100 ° C. for 3 hours in a reactor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, concentrated under reduced pressure, dissolved in purified water, and filtered through a deodorized 0.45 μm filter. Got.

Example 26

1 kg of shiitake mushrooms, which were washed with purified water and dried completely, were placed in 16 kg of water, and the pH was adjusted to 10 using an aqueous sodium carbonate solution, followed by extraction at 100 ° C. for 8 hours. The extract was centrifuged to give a supernatant, adjusted to pH 4, precipitated in 12 kg of ethanol, filtered and dried to give 50 g of lentinan. 50 g of lentinane was added to 2 kg of formic acid-2N hydrochloric acid (4: 1), heated in a reactor equipped with a cooling condenser for 3 hours at 100 ° C., filtered through a 400 mesh filter cloth, and concentrated under reduced pressure. Filtration gave 1.2 kg of aqueous solution.

Preparation of Cordyceps Sinensis Extract and Oligo Lentinan Mixtures

Example 27

1.0 g of Cordyceps sinensis extract solids of Example 1 were dissolved in 300 g of butylene glycol, and then added to the oligo-lentinan aqueous solution obtained in Example 22 to obtain 1 kg of the mixture.

Example 28-32

When dissolving Cordyceps sinensis extract solids, it was prepared in the same manner as in Example 27 using the solvent of Table 2 below, and the results are shown in Table 2 below.

Table 2

Example Number Solvent Amount of Solvent

Example 28 Butylene Glycol 500g

Example 29 300 g of propylene glycol

Example 30 500 g of propylene glycol

Example 31 300 g of ethanol

Example 32 500 g of ethanol

Cell proliferation effect experiment

100 μl of 5% calf serum (FBS) solution was added to a 96 microwell plate, then 2%

Samples were dispersed in DMEM medium containing FBS (phosphate buffer), diluted, and added again by 100 μl. Using a hemocytometer, fibroblasts obtained from foreskin tissue of newborns were dispersed in 100 μl medium per well and incubated for 7 days. After the incubation, the supernatant was removed and washed again by adding 200ul of 5% PBS, and 150µl of DMSO was added per well, followed by shaking for 10 minutes to measure absorbance at 570nm. At this time, the experiment was carried out in the same manner without adding the sample as a control to compare the cell proliferation effect from the relative difference in absorbance.

Table 3 shows the result of each example.

Table 3

Example Final concentration of Cordyceps Sinensis extract in the reaction solution (µg / ml) Final concentration of Oligo-Lentinan contained in the reaction solution (㎍ / ㎖) Cell proliferation effect One 10 - 130 2 10 - 119 3 10 - 115 4 10 - 121 5 10 - 125 6 10 - 11 7 10 - 122 8 10 - 122 9 10 - 125 10 10 - 119 11 10 - 129 12 10 - 120 13 10 - 129 14 10 - 129 15 10 - 126 16 10 - 124 17 10 - 127 18 10 - 120 19 10 - 120 20 10 - 119 21 10 - 123 22 - 10 115 23 - 10 118 24 - 10 117 25 - 10 117 26 - 10 115 27 5 5 132 28 5 5 135 29 5 5 132 30 5 5 129 31 5 5 130 32 5 5 132

In addition, the cell proliferation effect according to the concentration of Cordyceps sinensis extract of Example 16 in Fig. 1, the cell proliferation effect according to the concentration of oligo-lentinan of shiitake mushroom of Example 22 in Fig. Cell proliferation effects according to the concentration of the oligo-lentinan mixture are shown in FIG. 3.

Prescription Example

Prescription Example 1

Table 4 is a prescription example of the lotion (skin, lotion) in the cosmetic containing Cordyceps sinensis extract and oligo lentinan. Here, Cordyceps sinensis extract was used in Example 1, oligo lentinan was used in Example 22.

Table 4

number Raw material Prescription Example 1 Comparative Example 1 One Cordyceps Sinensis Extract 10.0 - 2 Oligo-Lentinan aqueous solution 10.0 - 3 glycerin 3.0 3.0 4 Butylene Glycol 2.0 2.0 5 Propylene glycol 2.0 2.0 6 Polyoxyethylene (60) Cured Castor Oil 1.00 1.0 7 ethanol 10.0 10.0 8 Triethanolamine 0.1 0.1 9 antiseptic a very small amount a very small amount 10 Pigment a very small amount a very small amount 11 Spices a very small amount a very small amount 12 Purified water Remaining amount Remaining amount

3, 4, 5, and 9 were added to 12 in Table 4 in order to dissolve it, and 6 was heated to 60 ° C. for dissolution, and 11 was added to 12 times with stirring. Lastly, 1,2,7,8,10 times were added and sufficiently stirred before aging.

Comparative Example 1

3, 4, 5, and 9 were added to 12 in Table 4 in order to dissolve it, and 6 was heated to 60 ° C. for dissolution, and 11 was added to 12 times with stirring. Finally, 7,8,10 times were added and sufficiently stirred before aging.

Prescription Example 2

Table 5 is a prescription example of nutritional lotion in cosmetics containing Cordyceps sinensis extract and oligo lentinan. Here, Cordyceps sinensis extract was used in Example 1, oligo lentinan was used in Example 22.

12, 13, 14, 16, and 19 of Table 5 were mixed and stirred at 80 to 85 ° C. and fed into the manufacturing unit, and then the emulsifier was operated. 3, 4, 5, 6, 7, 8, 9, 10, 11 and 15 were heated to 80 to 85 ° C., and then emulsified.

After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 17 times were added thereto, then cooled to 45 ° C., 18 times were added thereto, and 1,2 times were added to 35 ° C., cooled to 25 ° C., and aged.

Table 5

number Circle Prescription Example 2 Comparative Example 2 One Cordyceps Sinensis Extract 10.0 - 2 Oligo-Lentinan aqueous solution 10.0 - 3 Beeswax 10.0 1.0 4 Polysorbate 60 1.5 1.5 5 Sorbitan sesquioleate 0.5 0.5 6 Liquid paraffin 5.0 5.0 7 Squalane 5.0 5.0 8 Sorbitan stearate 1.00 1.00 9 Glyceryl Stearate / PEG-400 Stearate 1.00 1.00 10 Lipophilic monostearic acid glycerin 0.50 0.50 11 Stearic acid 1.50 1.50 12 Butylene glycol 5.00 5.00 13 Propylene glycol 5.00 5.00 14 Carboxy Vinyl Polymer 0.1 0.1 15 Triethanolamine 0.2 0.2 16 antiseptic a very small amount a very small amount 17 Pigment a very small amount a very small amount 18 Spices a very small amount a very small amount 19 Purified water Remaining amount Remaining amount

Comparative Example 2

12, 13, 14, 16, and 19 of Table 5 were mixed and stirred, heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier was operated. 3,4,5,6,7,8,9,10 , 11 and 15 were heated to 80 to 85 DEG C, and then emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 17 times were added thereto, then cooled to 45 ° C., 18 times were added thereto, cooled to 25 ° C., and aged.

Prescription Example 3

Table 6 below is a prescription example of nutrition cream in cosmetics containing Cordyceps sinensis extract and oligo lentinan. Here, Cordyceps sinensis extract was used in Example 1, oligo lentinan was used in Example 22.

Table 6

number Raw material Prescription Example 3 Comparative Example 3 One Cordyceps Sinensis Extract 10.0 - 2 Oligo-Lentinan aqueous solution 10.0 - 3 Beeswax 5.00 5.00 4 Polysorbate 60 1.5 1.5 5 Sorbitan sesquioleate 0.5 0.5 6 Liquid paraffin 10.0 10.0 7 Squalane 10.0 5.0 8 Sorbitan stearate 1.00 5.0 9 Glyceryl Stearate / Pig-400 Stearate 1.00 5.0 10 Lipophilic monostearic acid glycerin 0.50 0.50 11 Stearic acid 1.50 1.50 12 Butylene glycol 5.00 3.00 13 Propylene glycol 5.00 3.00 14 Triethanolamine 0.2 0.2 15 antiseptic a very small amount a very small amount 16 Pigment a very small amount a very small amount 17 Spices a very small amount a very small amount 18 Purified water Remaining amount Remaining amount

12, 13, 14, 15, and 18 of Table 6 were mixed and stirred, heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier was operated. And 11 were heated to 80 to 85 ° C. to be emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 16 times were added thereto. After cooling to 25 ℃ it was aged.

Comparative Example 3

12, 13, 14, 15, and 18 of Table 6 were mixed and stirred, heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier was operated. And 11 were heated to 80 to 85 ° C. to be emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 16 times were added thereto, then cooled to 45 ° C., 17 times were added thereto, cooled to 25 ° C., and aged.

Prescription Example 4

Table 7 below is a prescription example of a massage cream in cosmetics containing Cordyceps sinensis extract and oligo-lentinan. Here, Cordyceps sinensis extract was used in Example 1, oligo lentinan was used in Example 22.

9, 10, 11, 12, 14, and 17 in Table 7 were mixed and stirred, heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier was operated. Was heated to 80 to 85 ° C. and emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 15 times were added thereto, then cooled to 45 ° C. and 16 times were added thereto. After cooling to 25 ℃ it was aged.

Table 7

number Raw material Prescription Example 4 Comparative Example 4 One Cordyceps Sinensis Extract 10.0 - 2 Oligo-Lentinan aqueous solution 10.0 - 3 Beeswax 10.0 10.0 4 Polysorbate 60 1.5 1.5 5 Sorbitan sesquioleate 0.8 0.8 6 Liquid paraffin 40.0 40.0 7 Squalane 5.0 5.0 8 Caprylic / Capric Triglycerides 4.0 4.0 9 Carboxy Vinyl Polymer 0.2 0.2 10 glycerin 5.0 5.0 11 Butylene glycol 3.0 3.0 12 Propylene glycol 3.0 3.0 13 Triethanolamine 0.2 0.2 14 antiseptic a very small amount a very small amount 15 Pigment a very small amount a very small amount 16 Spices a very small amount a very small amount 17 Purified water Remaining amount Remaining amount

Comparative Example 4

9, 10, 11, 12, 14, and 17 in Table 7 were mixed and stirred, heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier was operated. Was heated to 80 to 85 ° C. and emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 15 times were added thereto, cooled to 45 ° C., 16 times, and cooled to 25 ° C., and aged.

Prescription Example 5

Table 8 below is a prescription example of nutritional essence in cosmetics containing Cordyceps sinensis extract and oligo-lentinan. Here, Cordyceps sinensis extract was used in Example 1, oligo lentinan was used in Example 22.

Table 8

number Raw material Prescription Example 5 Comparative Example 5 One Cordyceps Sinensis Extract 10.0 - 2 Oligo-Lentinan aqueous solution 10.0 - 3 Cytostolol 1.70 1.70 4 Polyglyceryl 2-oleate 1.50 1.50 5 Ceramide 0.7 0.7 6 Ceteares-4 1.2 1.2 7 cholesterol 1.5 1.5 8 Dicetylphosphate 0.4 0.4 9 Concentrated glycerin 5.0 5.0 10 Sunflower oil 15.0 15.0 11 Carboxy Vinyl Polymer 0.2 0.2 12 Xanthan Gum 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water Remaining amount Remaining amount

The raw materials 3,4,5,6 and 7 of Table 8 are homogenized at a constant temperature, the raw materials 1,2,8,9 and 15 are mixed and homogenized at a constant temperature, and then passed through a microfluidizer. The raw material 10 was added at a constant temperature, homogenized, and then passed again through a microfluidizer. And 11, 12, 13, 14 was added to disperse to stabilize and mature.

Comparative Example 5

The raw materials 3, 4, 5, 6 and 7 of Table 8 are homogenized at a constant temperature, mixed with the raw materials 8, 9 and 15, homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 10 is obtained. The solution was added slowly and homogenized at a constant temperature, and then passed through the microfluidizer again. And 11, 12, 13, 14 was added to disperse to stabilize and mature.

Prescription Example 6

Table 9 below is a prescription example of the emulsified foundation in cosmetics containing Cordyceps sinensis extract and oligo-lentinan. Here, Cordyceps sinensis extract was used in Example 1, oligo lentinan was used in Example 22.

10, 11, 12, 13, 14, 15, 16, and 18 in Table 9 were mixed and stirred, heated to 80-85 ° C., put into the manufacturing unit, and then the emulsifier was operated. , 8 and 9 were heated to 80 to 85 ° C., and then emulsified. After emulsification, cool down to 45 ℃ with stirring using a stirrer, add 17 times, cool down to 35 ℃, and add 1,2 times. After cooling to 25 ℃ it was aged.

Comparative Example 6

10, 11, 12, 13, 14, 15, 16, and 18 of Table 9 were heated to 80 to 85 ° C. while mixing and stirring, and then introduced into the manufacturing unit, followed by actuating an emulsifier. 3,4,5,6,7 , 8 and 9 were heated to 80 to 85 ° C., and then emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 17 times were added thereto, cooled to 25 ° C., and aged.

Table 9

number Raw material Prescription Example 6 Prescription Example 6 One Cordyceps Sinensis Extract 5.0 - 2 Oligo-Lentinan aqueous solution 5.0 - 3 Beeswax 2.0 2.0 4 Pyromethicone 2.0 2.0 5 Liquid paraffin 5.0 5.0 6 Squalane 5.0 5.0 7 Stearic acid 2.0 2.0 8 Lipophilic monostearic acid glycerin 3.0 3.0 9 Caprylic / Capric Triglycerides 4.0 4.0 10 glycerin 4.0 4.0 11 Propylene glycol 3.0 3.0 12 Butylene glycol 3.0 3.0 13 Triethanolamine 1.0 1.0 14 Aluminum Magnesium Silicate 0.5 0.5 15 Pigment 12 12 16 antiseptic a very small amount a very small amount 17 Spices a very small amount a very small amount 18 Purified water Remaining amount Remaining amount

Experiment to confirm skin condition improvement effect

In order to confirm the skin condition improvement effect when applying the skin to the nutritional cream of Formulation Example 3 and Comparative Example 3, the following clinical confirmation experiment. Fifty-five owners of normal, oily, dry, and complex skin were composed of 25% of women aged 20 to 55 years, and their skin condition was improved. To the same person, the nutrition cream of prescription example 3 was applied to the upper part of the face, and the nutrition cream of comparative example 3 was applied to the skin once a day for 20 days once a day after washing the face, and then the skin condition was improved to confirm the improvement of the skin condition. The degree was measured. The test results are shown in Table 10.

Table 10

Investigation Item Skin condition improvement Remarks Sample Number of people % Example very good 33 66.0 good 12 24.0 is average 5 10.0 so so - - Comparative Example 3 Good actor - - good 7 140. is average 18 36.0 so so 25 50.0

As a result of the investigation, it can be seen that the skin improvement effect is superior to that of Comparative Example 3 prepared without adding the cosmetics of Formula 3 prepared by adding Cordyceps sinensis extract and oligo-lentinan extract.

Increased elasticity test on the skin

In order to measure the effect of increasing the elasticity of the skin when the nutrition lotions of Formulation Example 2 and Comparative Example 2 were applied to the skin, the left side of the face of 20 women aged 20 to 55 years of age with normal, oily, dry and combination skin Skin elasticity measurement after 1 week, 2 weeks, 3 weeks and 4 weeks after the application of the nutrition lotion of Example 2 to the part and the nutrition lotion of Comparative Example 2 on the right side of the face twice daily in the morning and evening. The elasticity of the skin was measured using a ballistometer. The measurement results are shown in Table 11 and FIG. 4.

Table 11

Cosmetics of Prescription Example 2 Cosmetics of Comparative Example 2 Remarks T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄ Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 amplitude first 1.9 6.1 6.4 6.9 7.8 1.9 3.0 3.5 3.7 3.9 second 1.3 4.6 5.7 6.2 6.9 1.6 2.0 2.7 2.9 3.0 third 0.0 3.4 4.0 5.4 6.2 0.0 0.0 2.0 2.3 2.7 fourth 0.0 0.0 2.6 4.7 5.5 0.0 0.0 0.0 0.0 0.0 Average 0.8 3.525 4.675 5.80 6.60 0.80 1.25 2.05 2.225 2.40

(Note) T0: Before use, after T 1: 1 injection, after T 2: 3 weeks, after T 3: 3 weeks, after T 4: 4 weeks

As described above, the nutritional lotion of Formulation Example 2 containing Cordyceps sinensis extract and oligo-lentinan extract according to the present invention has skin elasticity as compared to the nutrition lotion of Comparative Example 2 which does not contain Cordyceps sinensis extract and oligo-lentinan extract. It can be seen that it produces a very good effect to improve the.

The extract having a cell proliferation effect extracted by the present invention as described above is excellent in skin absorption, the composition of the cosmetic containing such an extract shows an effect of increasing skin elasticity due to the proliferative effect of skin cells. In addition, it is extracted from natural substances and has excellent skin stability and has the advantage of being non-irritating to the skin.

Claims (12)

A cosmetic composition comprising oligo lentinane and Cordyceps sinensis extract obtained by acid treatment of lentinan extracted from shiitake mushrooms. According to claim 1, Cordyceps sinensis extract is a cosmetic composition, characterized in that extracted by heating for 3-20 hours at 50-100 ℃ or 1-15 days at 10-37 ℃ by adding a solvent to Cordyceps sinensis. According to claim 2, wherein the solvent is one or more solvents selected from the group consisting of water, ethanol, methanol, propanol, butanol, acetone, hexane, benzene, chloroform, glycerin, ethylene glycol, propylene glycol, butylene glycol Cosmetic composition characterized in that. The cosmetic composition according to claim 2, wherein the solvent is used in 10-25 times the volume of Cordyceps sinensis. The method of claim 1, wherein the oligo lentinane is added to an extraction solvent of pH 7-10, extracted for 4 to 48 hours at 60-100 ℃ or immersed for 1-15 days at 5-37 ℃, the precipitation solvent A cosmetic composition, characterized in that the solid content obtained by precipitating in water is decomposed into an acid and then neutralized. 6. The cosmetic composition according to claim 5, wherein the extraction solvent is a phosphate buffer solution or an aqueous sodium carbonate solution. The cosmetic composition according to claim 6, wherein the extraction solvent is used in a volume of 5 to 30 times the amount of shiitake mushrooms. The cosmetic composition according to claim 5, wherein the precipitation solvent is ethanol. 6. The cosmetic composition according to claim 5, wherein the acid decomposition is performed by heating to 80-100 DEG C in at least one acid solution selected from the group consisting of dilute hydrochloric acid, dilute sulfuric acid, dilute nitric acid, formic acid and acetic acid. The method of claim 2, wherein the Cordyceps sinensis extract is a solution dissolved in one or more solvents selected from the group consisting of ethanol, butylene glycol and propylene glycol, wherein oligo-lentinan is added to the cosmetic composition in the form of an aqueous solution dissolved in water. Cosmetic composition to be. The cosmetic composition according to claim 2, wherein the cosmetic composition has a formulation of a lotion, a nutrition lotion, a nutrition cream, a massage cream, a nutrition essence, or an emulsified foundation. 3. The cosmetic composition according to claim 2, wherein the Cordyceps sinensis extract and oligo-lentinan are contained in an amount of 0.0001-5% by weight in dry weight.